JP2000245492A5 - - Google Patents
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- JP2000245492A5 JP2000245492A5 JP1999053766A JP5376699A JP2000245492A5 JP 2000245492 A5 JP2000245492 A5 JP 2000245492A5 JP 1999053766 A JP1999053766 A JP 1999053766A JP 5376699 A JP5376699 A JP 5376699A JP 2000245492 A5 JP2000245492 A5 JP 2000245492A5
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- extracted lipid
- extracted
- lipid according
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 150000002632 lipids Chemical class 0.000 description 29
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 244000005700 microbiome Species 0.000 description 12
- 150000003904 phospholipids Chemical class 0.000 description 7
- -1 aminoacyl phospholipid Chemical class 0.000 description 5
- 240000008371 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 4
- 239000002502 liposome Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002798 polar solvent Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- ZAKBPRIDLSBYQQ-VHSXEESVSA-N 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) Chemical compound CC(=O)OC[C@@H](OC(C)=O)COP(O)(=O)OC[C@@H](O)CO ZAKBPRIDLSBYQQ-VHSXEESVSA-N 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- 229940075615 Bacillus subtilis Drugs 0.000 description 2
- 241000194031 Enterococcus faecium Species 0.000 description 2
- 229940004208 Lactobacillus bulgaricus Drugs 0.000 description 2
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 2
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 2
- 229940017800 Lactobacillus casei Drugs 0.000 description 2
- 240000004403 Lactobacillus casei Species 0.000 description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 description 2
- 108091005771 Peptidases Proteins 0.000 description 2
- 102000015439 Phospholipases Human genes 0.000 description 2
- 108010064785 Phospholipases Proteins 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- FGYYWCMRFGLJOB-MQWKRIRWSA-N [2,3-dihydroxypropoxy(hydroxy)phosphoryl] (2S)-2,6-diaminohexanoate Chemical compound NCCCC[C@H](N)C(=O)OP(O)(=O)OCC(O)CO FGYYWCMRFGLJOB-MQWKRIRWSA-N 0.000 description 2
- 235000019833 protease Nutrition 0.000 description 2
- 101700024603 ANNU Proteins 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 229940075612 Bacillus cereus Drugs 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 102000033147 ERVK-25 Human genes 0.000 description 1
- 241000520130 Enterococcus durans Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 108060003339 GPLD1 Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 229940039696 Lactobacillus Drugs 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 229940039695 Lactobacillus acidophilus Drugs 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 241000186840 Lactobacillus fermentum Species 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 229940072205 Lactobacillus plantarum Drugs 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 102000035443 Peptidases Human genes 0.000 description 1
- 102000011420 Phospholipase D Human genes 0.000 description 1
- 108090000553 Phospholipase D Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229940030998 Streptococcus agalactiae Drugs 0.000 description 1
- 241000193985 Streptococcus agalactiae Species 0.000 description 1
- 101700034322 TGAS Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940012969 lactobacillus fermentum Drugs 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 101700012968 tgl Proteins 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 微生物の菌体より抽出され、かつ塩基性リン脂質を含むリン
脂質を１０重量％以上含有する微生物抽出脂質。
【請求項２】 塩基性リン脂質を０．５重量％以上含む請求項１記載の微生
物抽出脂質。
【請求項３】 塩基性リン脂質が塩基性アミノアシルリン脂質である請求項
１または請求項２記載の微生物抽出脂質。
【請求項４】 塩基性アミノアシルリン脂質がホスファチジルグリセロール
の塩基性アミノ酸エステルである請求項３記載の微生物抽出脂質。
【請求項５】 ホスファチジルグリセロールの塩基性アミノ酸エステルがリ
ジルホスファチジルグリセロールまたはオルニチルホスファチジルグリセロール
である、請求項４記載の微生物抽出脂質。
【請求項６】 微生物が菌体内に塩基性リン脂質を含有する微生物である、
請求項１〜５のいずれか一項に記載の微生物抽出脂質。
【請求項７】 微生物が細菌、酵母またはカビである、請求項１〜６のいず
れか一項に記載の微生物抽出脂質。
【請求項８】 微生物がバチルス（Bacillus）属、ストレプトコッカス（St
reptococcus）属およびラクトバチルス（Lactobacillus）属から選ばれる属に属
する微生物である、請求項１〜６のいずれか一項に記載の微生物抽出脂質。
【請求項９】 微生物がバチルス・ズブチルス（Bacillus subtilis）、バ
チルス・ナットウ（Bacillus natto）、バチルス・メガテリウム(Bacillus mega
terium）、バチルス・リチェニフォルミス（Bacillus licheniformis）、バチル
ス・セレウス（Bacillus cereus）、ストレプトコッカス・フェカリス（Strepto
coccus faecalis)、ストレプトコッカス・デュランス（Streptococcus durans）
、ストレプトコッカス・ファシウム（Streptococcus faecium）、ストレプトコ
ッカス・アガラクティア（Streptococcus agalactiae）、ラクトバチルス・ブル
ガリカス（Lactobacillus bulgaricus）、ラクトバチルス・カゼイ（Lactobacil
lus casei）、ラクトバチルス・プランタラム（Lactobacillus plantarum）、ラ
クトバチルス・ファーメンタム（Lactobacillus fermentum）、ラクトバチルス
・ラクティス（Lactobacillus lactis）およびラクトバチルス・アシドフィラス
（Lactobacillus acidophilus）から選ばれる微生物である、請求項１〜６のい
ずれか一項に記載の微生物抽出脂質。
【請求項１０】 微生物の菌体より極性溶媒を用いて抽出される、請求項１
〜９のいずれか一項に記載の微生物抽出脂質。
【請求項１１】 極性溶媒がエタノール、メタノール、アセトン、ジエチル
エーテルまたはクロロホルムから選ばれる１種以上の溶媒である、請求項１０記
載の微生物抽出脂質。
【請求項１２】 請求項１〜９のいずれか一項に記載の微生物抽出脂質の製
造方法であって、微生物を培養して得られる菌体より極性溶媒を用いて抽出する
ことを特徴とする微生物抽出脂質の製造方法。
【請求項１３】 溶媒がエタノール、メタノール、アセトン、ジエチルエー
テルまたはクロロホルムから選ばれる１種以上の溶媒である、請求項１２記載の
微生物抽出脂質の製造方法。
【請求項１４】 請求項１から請求項１１までのいずれか一項に記載の微生
物抽出脂質を酵素で処理して得られる酵素処理微生物抽出脂質。
【請求項１５】 酵素がホスホリパーゼＡ １ 、ホスホリパーゼＡ2 、ホスホ
リパーゼＣおよびホスホリパーゼＤから選ばれる１種以上の酵素である、請求項
１４記載の酵素処理微生物抽出脂質。
【請求項１６】 酵素がペプチダーゼ、プロテアーゼおよびトランスグルタ
ミナーゼから選ばれる１種以上の酵素である請求項１４記載の酵素処理微生物抽
出脂質。
【請求項１７】 請求項１〜１１のいずれか一項に記載の微生物抽出脂質ま
たは請求項１４〜１６のいずれか一項に記載の酵素処理微生物抽出脂質を含有す
る界面活性剤。
【請求項１８】 請求項１〜１１のいずれか一項に記載の微生物抽出脂質ま
たは請求項１４〜１６のいずれか一項に記載の酵素処理微生物抽出脂質を含有す
る乳化剤。
【請求項１９】 請求項１〜１１のいずれか一項に記載の微生物抽出脂質ま
たは請求項１４〜１６のいずれか一項に記載の酵素処理微生物抽出脂質を含有す
る澱粉老化抑制剤。
【請求項２０】 請求項１〜１１のいずれか一項に記載の微生物抽出脂質ま
たは請求項１４〜１６のいずれか一項に記載の酵素処理微生物抽出脂質を含有す
る抗微生物剤。
【請求項２１】 請求項１〜１１のいずれか一項に記載の微生物抽出脂質ま
たは請求項１４〜１６のいずれか一項に記載の酵素処理微生物抽出脂質を含有す
るリポソーム基材。
【請求項２２】 請求項１〜１１のいずれか一項に記載の微生物抽出物質ま
たは請求項１４〜１６のいずれか一項に記載の酵素処理微生物抽出脂質から精製
して得られる塩基性リン脂質を含有するリポソーム基材。
【請求項２３】 塩基性リン脂質がリジルホスファチジルグリセロールであ
る請求項２２記載のリポソーム基材。
[Claims]
1. A microorganism-extracted lipid which is extracted from cells of a microorganism and contains 10% by weight or more of a phospholipid containing a basic phospholipid.
2. The microorganism-extracted lipid according to claim 1, which contains a basic phospholipid in an amount of 0.5% by weight or more.
3. The microorganism-extracted lipid according to claim 1, wherein the basic phospholipid is a basic aminoacyl phospholipid.
4. The microorganism-extracted lipid according to claim 3, wherein the basic aminoacyl phospholipid is a basic amino acid ester of phosphatidylglycerol.
5. The microorganism-extracted lipid according to claim 4, wherein the basic amino acid ester of phosphatidyl glycerol is lysyl phosphatidyl glycerol or ornithyl phosphatidyl glycerol.
6. The microorganism according to claim 1, wherein the microorganism contains a basic phospholipid in the cells.
A microorganism-extracted lipid according to any one of claims 1 to 5.
7. The microorganism-extracted lipid according to any one of claims 1 to 6, wherein the microorganism is a bacterium, yeast or mold.
8. The microorganisms of the genus Bacillus , Streptococcus ( St.
The microorganism-extracted lipid according to any one of claims 1 to 6, which is a microorganism belonging to a genus selected from the genus reptococcus ) and the genus Lactobacillus .
9. The microorganism Bacillus subtilis (Bacillus subtilis), Bacillus natto (Bacillus natto), Bacillus megaterium (Bacillus mega
terium ), Bacillus licheniformis , Bacillus cereus , Streptococcus faecalis ( Strepto)
coccus faecalis ), Streptococcus durans
, Streptococcus faecium (Streptococcus faecium), Streptococcus Agarakutia (Streptococcus agalactiae), Lactobacillus bulgaricus (Lactobacillus bulgaricus), Lactobacillus casei (Lactobacil
lus casei ), Lactobacillus plantarum , Lactobacillus fermentum , Lactobacillus lactis and Lactobacillus acidophilus. The microorganism-extracted lipid according to any one of 1 to 6.
10. The method according to claim 1, wherein the microorganism is extracted from the cells of the microorganism using a polar solvent.
10. The microorganism-extracted lipid according to any one of claims 9 to 9.
11. The microorganism-extracted lipid according to claim 10, wherein the polar solvent is one or more solvents selected from ethanol, methanol, acetone, diethyl ether and chloroform.
12. The method for producing a microorganism-extracted lipid according to any one of claims 1 to 9, wherein the lipid is extracted from a cell obtained by culturing the microorganism using a polar solvent. A method for producing a microorganism-extracted lipid.
13. The method for producing a microorganism-extracted lipid according to claim 12, wherein the solvent is one or more solvents selected from ethanol, methanol, acetone, diethyl ether, and chloroform.
14. An enzyme-treated microorganism-extracted lipid obtained by treating the microorganism-extracted lipid according to any one of claims 1 to 11 with an enzyme.
15. The lipid extracted from an enzyme-treated microorganism according to claim 14, wherein the enzyme is one or more enzymes selected from phospholipase A 1 , phospholipase A 2 , phospholipase C and phospholipase D.
16. The lipid according to claim 14, wherein the enzyme is at least one enzyme selected from peptidase, protease and transglutaminase.
17. A surfactant containing the microorganism-extracted lipid according to any one of claims 1 to 11 or the enzyme-treated microorganism-extracted lipid according to any one of claims 14 to 16.
18. An emulsifier containing the microorganism-extracted lipid according to any one of claims 1 to 11 or the enzyme-treated microorganism-extracted lipid according to any one of claims 14 to 16.
19. A starch aging inhibitor comprising the microorganism-extracted lipid according to any one of claims 1 to 11 or the enzyme-treated microorganism-extracted lipid according to any one of claims 14 to 16.
20. An antimicrobial agent comprising the microorganism-extracted lipid according to any one of claims 1 to 11 or the enzyme-treated microorganism-extracted lipid according to any one of claims 14 to 16.
21. A liposome substrate comprising the microorganism-extracted lipid according to any one of claims 1 to 11 or the enzyme-treated microorganism-extracted lipid according to any one of claims 14 to 16.
22. A basic phospholipid obtained by purifying the microorganism-extracted substance according to any one of claims 1 to 11 or the lipid-extracted enzyme-treated microorganism according to any one of claims 14 to 16. A liposome substrate containing
23. The liposome substrate according to claim 22, wherein the basic phospholipid is lysylphosphatidylglycerol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11053766A JP2000245492A (en) | 1999-03-02 | 1999-03-02 | Lipid extracted from microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11053766A JP2000245492A (en) | 1999-03-02 | 1999-03-02 | Lipid extracted from microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000245492A JP2000245492A (en) | 2000-09-12 |
| JP2000245492A5 true JP2000245492A5 (en) | 2006-02-16 |
Family
ID=12951947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11053766A Pending JP2000245492A (en) | 1999-03-02 | 1999-03-02 | Lipid extracted from microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2000245492A (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1252324B1 (en) | 2000-01-19 | 2010-10-20 | Martek Biosciences Corporation | Solventless extraction process |
| AU2005217300B2 (en) * | 2004-03-01 | 2010-12-02 | Suntory Holdings Limited | Process for producing phospholipid containing long chain polyunsaturated fatty acid as constituent thereof and utilization of the same |
| JP5911479B2 (en) | 2010-06-01 | 2016-04-27 | ディーエスエム アイピー アセッツ ビー．ブイ． | Extraction of lipids from cells and products derived therefrom |
| SG11201605052XA (en) | 2013-12-20 | 2016-07-28 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
| KR102435268B1 (en) * | 2013-12-20 | 2022-08-22 | 디에스엠 뉴트리셔널 프라덕츠 아게 | Methods of recovering oil from microorganisms |
| US11124736B2 (en) | 2013-12-20 | 2021-09-21 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| JP2017501709A (en) | 2013-12-20 | 2017-01-19 | ディーエスエム アイピー アセッツ ビー．ブイ． | Method for obtaining microbial oil from microbial cells |
| NZ721417A (en) | 2013-12-20 | 2022-07-01 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
| CN115141859A (en) | 2013-12-20 | 2022-10-04 | 玛拉可再生能源公司 | Method for recovering oil from microorganisms |
| CN109609567B (en) * | 2018-12-30 | 2022-05-03 | 新疆阜丰生物科技有限公司 | Green production method of L-tryptophan by using mycoprotein enzymolysis liquid to replace yeast powder |
-
1999
- 1999-03-02 JP JP11053766A patent/JP2000245492A/en active Pending
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