JP2000229245A - Microchemical reaction device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for efficiently and accurately introducing chemical materials on plural fine chemical reaction electrode dots for a short time by fine molecular synthetic and recognition method and to provide a method for efficiently performing reaction control and product detection. SOLUTION: A device composed of an optional electrode substrate circuit, a finely structured mechanical vibration membrane provided on the electrode and a thin film for supplying heat and many micropixel pots, each of which is provided with a nozzle to enable to sample a trace liquid at the lower part and is capable of controlling the chemical reaction, is constituted to form a microchemical reaction device system for simultaneously performing many chemical reactions for a short time by accurately introducing raw material solution or the like, on the surface of a required microreaction pot 13 in the microdot from an ink jet nozzle 11 to supply raw materials, solvent or the like necessary for the chemical reactions to each reaction pixel pot. As the mechanical vibration membrane, a PZE piezo thin membrane 14 is used and an efficient stirring structure is introduced.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus for synthesizing and detecting a trace substance, and more particularly to a material reaction apparatus capable of inducing a plurality of different chemical substances by a mechanical method and detecting a minute chemical reaction in real time.

[0002]

2. Description of the Related Art In the field of the chemical industry, the synthesis of a chemical substance is conventionally performed by preparing a plurality of reaction starting materials and necessary reaction reagents or catalysts and mixing and introducing them into one reaction vessel or reaction apparatus. , Heating, light, etc., to promote the chemical reaction. In this method, however, 1
Only one reaction can proceed with the reactor. Moreover, it is difficult to easily control and monitor the progress of the reaction from outside the reaction vessel. Another problem in chemical synthesis is that the target product is not always obtained under the set reaction conditions in order to synthesize the target material at the research and development stage. In particular, the synthetic pathway of molecules designed on a desk is 100
It is not reliable, and in many cases, even if synthesis is attempted, it is not the intended product in many cases, and trial and error experiments are required until finally determining the conditions. At that time, a large amount of starting material chemicals are wasted and wasteful by-products are synthesized by the end of the optimization experiment.
The consumption of these wasteful resources and the disposal of by-products, which emerge from the research and synthesis process from the viewpoint of recent global environmental problems, have become major problems.

[0003]

In view of the above-mentioned problems, the present invention introduces a method of synthesizing and recognizing a trace amount of a molecule different from the conventional one, and provides a different means for supplying starting materials necessary for the synthesis. It introduces a method.

[0004] That is, an object of the present invention is to provide a method for accurately and accurately introducing a chemical material onto a plurality of minute chemical reaction electrode dots in a short time by a new method for synthesizing and recognizing a trace molecule. It is an object of the present invention to provide a method for efficiently controlling a chemical reaction and detecting a product.

[0005]

According to the first aspect of the present invention, a mechanical vibration film having a microstructure provided on an arbitrary electrode substrate circuit and an electrode, a thin film for supplying heat, and a small amount of liquid are provided. A nozzle that can be sampled is provided at the bottom, and in a device consisting of a large number of micropixel pots that can control the chemical reaction, the raw material and solvent necessary for the chemical reaction are supplied from the inkjet nozzle to supply each reaction pixel pot. A microchemical reaction device is characterized in that a raw material solution or the like is introduced on the surface of a microreaction pot precisely required by microdots and a large number of chemical reactions are performed simultaneously.

According to this configuration, a method for efficiently and accurately introducing a chemical material in a short time is provided, and the control of those chemical reactions and the detection of products can be efficiently performed.

According to a second aspect of the present invention, in the microchemical reaction device, the microscopic mechanical vibration film according to the first aspect is formed of a piezo thin film composition. It is a structure of a chemical reaction device. According to this configuration, there is an effect that the chemical reaction that proceeds in the minute reaction pot progresses efficiently.

According to the third aspect of the present invention, in the microchemical reaction device, a change in a reactant is introduced by introducing light into the sensing of a chemical reaction occurring in the small reaction pot according to the first aspect. This is a configuration of a microchemical reaction device characterized by analyzing from absorption characteristics. According to this configuration, there is an effect that control of a chemical reaction proceeding in the small reaction pot and detection of a product can be efficiently performed.

[0009]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram showing a first embodiment of the present invention.

FIGS. 1, 2, and 3 are partial views illustrating the structure of a sensor device according to an embodiment. FIG. 1 shows a schematic diagram of an ink jet head for supplying a solution of a trace chemical substance and a minute chemical reaction. 10 is an inkjet head, 11 is a head nozzle for ejecting ink droplets,
12 is a flying droplet of a chemical reaction raw material solution, 13 is a minute reaction pixel formed on a substrate, 14 is a PZT thin film and a heating unit formed at the bottom of the minute reaction pixel, and 15 is a sample of the reaction solution as needed. Is a suction nozzle to be supplied to the micro sample analyzer. The ink jet head is of a type in which a piezo piezoelectric element is attached. When an electric signal is sent from the driving circuit to the piezoelectric element, the piezoelectric element is deformed and the liquid inside is pushed out by the deformed pressure, and the liquid is discharged from the nozzle.

In this case, the solution of the chemical reaction raw material is discharged as a microdot solution from the ink jet nozzle,
The raw material solution or the raw material itself, which has flown onto the patterned microchemical reaction pixel pot, accurately reaches and is fixed as droplets. The raw material solution that can be supplied here may be any as long as it is soluble in the solvent used for the reaction. At this time, the concentration of the raw material in the solution is desirably a viscosity within a condition that allows the solution to fly with an ink jet head. The raw material composition and the solvent mixed and introduced into the reaction pot are uniformly mixed by the stirring effect by operating the PZT diaphragm formed on the substrate at a required frequency, and are installed beside the diaphragm. The energy required for the reaction is supplied by the heated heater.
FIG. 2 is a view showing the configuration of a transparent substrate that seals each reaction pixel on a reaction chip substrate after a raw material solution is introduced into and fixed to a predetermined reaction pixel, and a sensor device that can monitor a reaction process from above with a transparent substrate. . Reference numeral 20 denotes an optical sensor device, which irradiates light of a specific wavelength toward each reaction pixel, and absorbs and analyzes the reflected light with a CCD to observe the reaction progress state. Especially during the reaction,
When a reactant exhibiting specific absorption for UV visible light or infrared light is generated, the reaction state in the middle can be observed semi-quantitatively based on the concentration of generated molecules responding to light. 21 is a cover for sealing each reaction pot. In this method, after the raw material molecules required for the reaction are introduced into the reaction pixel pot by the ink jet nozzle, the cover is accurately brought into close contact with each micro reaction pot and sealed. During the heating reaction, the reaction can proceed in each of the pots while blocking external influences. The cover is a quartz glass substrate coated with a Teflon thin film and has a thickness of about 100 μm to 0.1 mm. Reference numeral 22 is covered with a transparent thin-film glass so that the inside can be seen from the contact cover attached to the micro reaction chip. Reaction sensing by light becomes possible from this micro-sized window. As the reaction proceeds, a very small amount of the reactant is collected from the sampling nozzle 13 shown in FIG. 1 in real time, sent to a necessary analytical instrument directly from the microreaction chip, and the component analysis is performed instantaneously. For example,
The sampled minute sample can be analyzed by HPLC, and the presence or absence of the target product can be recognized by a microcapillary TLC system. FIG. 3 is a schematic diagram showing the entire configuration of the micro-reaction device. 3
Numeral 0 denotes an ink jet head capable of accurately supplying only a necessary amount of a raw material to a reaction pot, which is capable of introducing a raw material while scanning over a large number of reaction pots 31 formed on a chip substrate at a high speed. Reference numeral 32 denotes a cover that can hermetically seal the upper surface of the chip substrate formed of a reaction pot, and has a configuration in which a window that can be accurately observed by an optical device is provided on each reaction pot as shown in the figure. Reference numeral 33 denotes a device capable of monitoring a reaction, in which light of a specific wavelength is introduced into a reaction pot from an observation window, and the interaction with a reactant is observed with a CCD sensor.

[0012]

According to the present invention, a new method for synthesizing and recognizing trace molecules, which is different from the conventional method, enables a small amount of chemical material to be accurately and accurately deposited on a plurality of minute chemical reaction electrode dots in a short time. And a method for efficiently controlling those chemical reactions and detecting the product can be provided.

[Brief description of the drawings]

FIG. 1 is a schematic view showing a method for introducing a raw material necessary for chemical synthesis by an ink jet method into a micro reaction pot according to the present invention.

FIG. 2 is a schematic diagram showing a mechanism for performing a reaction in a microchemical reaction pot and controlling the chemical reaction in the present invention.

FIG. 3 is a schematic view showing an entire configuration of a trace chemical reaction device in the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 10 Ink-jet head 11 Ink-jet droplet flying nozzle 12 Flying droplet 13 Micro chemical reaction pixel pot 14 Heating heater and PZT vibration thin film 15 Sample taking nozzle 20 Light source and CCD sensor 21 Adhesive cover 22 Observation window 30 Ink-jet head 31 Micro-reaction pot 32 Adhesion Cover 33 Line type light source and CCD sensor

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) G01N 21/75 G01N 21/75 Z F-term (Reference) 2G054 AA02 EA04 EA05 FA06 FA33 FA40 GA01 GA02 GB10 4G057 AB21 AB31 AB34 4G068 AA06 AB11 AC17 AD23 AD49 4G075 AA01 AA39 AA62 BD15 CA32 CA80 EB01 EC01 ED15 EE13 FA05 FA08 FA11

Claims (3)

[Claims] 1. An arbitrary electrode substrate circuit and a mechanical vibration film having a minute structure and a thin film for supplying heat provided on an electrode, and a nozzle capable of sampling a trace amount of liquid are provided at a lower portion to control a chemical reaction. In a device consisting of a large number of possible micropixel pots, a micro-reaction pot that requires the raw material solution etc. in microdots precisely from the inkjet nozzle to supply the raw material, solvent, etc. necessary for the chemical reaction to each reaction pixel pot A microchemical reaction device characterized in that a large number of chemical reactions are simultaneously performed by being introduced on a surface. 2. The microchemical reaction device according to claim 1,
A microchemical reaction device, wherein the mechanical vibration film having a microstructure according to claim 1 is formed of a piezo thin film composition. 3. The microchemical reaction device according to claim 2,
A microchemical reaction device, characterized in that a change in a reactant is analyzed from its absorption characteristics by introducing light into the sensing of a chemical reaction occurring in the microreaction pot according to claim 1.