JP2000187030A5 - - Google Patents
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- JP2000187030A5 JP2000187030A5 JP1999008578A JP857899A JP2000187030A5 JP 2000187030 A5 JP2000187030 A5 JP 2000187030A5 JP 1999008578 A JP1999008578 A JP 1999008578A JP 857899 A JP857899 A JP 857899A JP 2000187030 A5 JP2000187030 A5 JP 2000187030A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- hair
- coexistence
- proliferation
- index
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004209 Hair Anatomy 0.000 description 3
- 210000004027 cells Anatomy 0.000 description 3
- 210000000442 hair follicle cell Anatomy 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 210000003135 Vibrissae Anatomy 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
Description
【特許請求の範囲】
【請求項1】 動物の毛乳頭細胞と毛包細胞とを共存させて培養し、その増殖の度合いを指標とすることを特徴とする、育毛剤のイン・ビトロの評価法。
【請求項2】 毛乳頭細胞と毛包細胞が髭由来の細胞であることを特徴とする、請求項1に記載の育毛剤のイン・ビトロの評価法。
【請求項3】 更に、毛包細胞のみを単独培養させて、この増殖の度合いを測定し、共存下での培養との差を指標とすることを特徴とする、請求項1又は2に記載の育毛剤の評価法。
[Claims]
1. A method for in vitro evaluation of a hair restorer, comprising culturing hair papilla cells and hair follicle cells in the coexistence of an animal and using the degree of proliferation as an index.
2. The method according to claim 1, wherein the dermal papilla cells and the hair follicle cells are cells derived from whiskers.
3. The method according to claim 1, wherein the hair follicle cells are solely cultured, the degree of the proliferation is measured, and the difference from the culture in the coexistence is used as an index. Hair restorer evaluation method.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP00857899A JP4257879B2 (en) | 1998-01-19 | 1999-01-18 | Evaluation method of hair restorer |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2152098 | 1998-01-19 | ||
JP10-21520 | 1998-01-19 | ||
JP00857899A JP4257879B2 (en) | 1998-01-19 | 1999-01-18 | Evaluation method of hair restorer |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2000187030A JP2000187030A (en) | 2000-07-04 |
JP2000187030A5 true JP2000187030A5 (en) | 2006-03-02 |
JP4257879B2 JP4257879B2 (en) | 2009-04-22 |
Family
ID=26343119
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP00857899A Expired - Fee Related JP4257879B2 (en) | 1998-01-19 | 1999-01-18 | Evaluation method of hair restorer |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP4257879B2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070128172A1 (en) * | 2003-12-05 | 2007-06-07 | Katsutoshi Yoshizato | Hair growth method |
US7985537B2 (en) | 2007-06-12 | 2011-07-26 | Aderans Research Institute, Inc. | Methods for determining the hair follicle inductive properties of a composition |
JP2014204711A (en) * | 2013-03-19 | 2014-10-30 | 国立大学法人 千葉大学 | Method for production of three-dimensional culture skin model, and use thereof |
-
1999
- 1999-01-18 JP JP00857899A patent/JP4257879B2/en not_active Expired - Fee Related
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