JP2000166537A - Divided culture medium - Google Patents
Divided culture mediumInfo
- Publication number
- JP2000166537A JP2000166537A JP28657098A JP28657098A JP2000166537A JP 2000166537 A JP2000166537 A JP 2000166537A JP 28657098 A JP28657098 A JP 28657098A JP 28657098 A JP28657098 A JP 28657098A JP 2000166537 A JP2000166537 A JP 2000166537A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- fractionation
- bulkhead
- partition
- upper bottom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、単一種又は複数種
の培地が注入されている分画培地に関し、特にゲル化の
際及び保存時の乾燥により培地が収縮することを防止す
る剥離防止手段を有する分画培地に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fractionation medium into which a single or plural kinds of culture mediums are injected, and more particularly, means for preventing peeling of the culture medium from shrinking due to drying during gelation and storage. And a fractionation medium having
【０００２】[0002]
【従来の技術】単一種又は複数種の培地が注入されてい
る分画培地は、同時に複数の検査を行うことができる点
で優れている。特に、米国特許３，９０２，９７２号明
細書及び特開平７−３０３４７０号公報記載の分画培地
は、隔壁よりも培地が高く盛り上げられているので隔壁
に引っかかることなく一度に試料を複数の培地に塗布す
ることができ、また、培地が収縮して隔壁や側壁から剥
離しないように剥離防止手段が設けられている。2. Description of the Related Art A fractionated medium into which a single or a plurality of types of media has been injected is excellent in that a plurality of tests can be performed simultaneously. In particular, in the fractionation medium described in U.S. Pat. No. 3,902,972 and JP-A-7-303470, since the medium is raised higher than the septum, a sample can be sampled into a plurality of mediums at once without being caught on the septum. In addition, a separation preventing means is provided to prevent the culture medium from shrinking and separating from the partition walls and side walls.
【０００３】[0003]
【発明が解決しようとする課題】しかし、米国特許３，
９０２，９７２号明細書及び特開平７−３０３４７０号
公報に示された剥離防止手段は、底面や側壁等から突出
した部材であり、剥離防止効果が十分でなく、保存状況
によっては培地が収縮して剥離することもあった。した
がって、本発明の目的は、十分な剥離防止効果を奏し、
観察しやすく、かつ製造が容易な分画培地を提供するこ
とである。However, US Pat.
The peeling prevention means disclosed in 902,972 and JP-A-7-303470 is a member protruding from the bottom surface or the side wall and the like, and does not have a sufficient peeling prevention effect. And peeled off. Therefore, the object of the present invention is to provide a sufficient peeling prevention effect,
An object of the present invention is to provide a fractionation medium that is easy to observe and easy to manufacture.
【０００４】[0004]
【課題を解決するための手段】本発明は、各区画の上底
面が、それを囲む隔壁及び内側壁の根元で連続的に窪ん
でおり、かつ該底面が平坦である分画培地である。The present invention is a fractionation medium in which the upper and lower surfaces of each section are continuously depressed at the base of the partition wall and the inner wall surrounding it, and the bottom is flat.
【０００５】また、本発明は、各区画の上底面が、それ
を囲む隔壁及び内側壁の根元で連続的に窪んでおり、か
つ該底面に非格子状の突起物及び／又は窪み部が設けら
れている分画培地である。Further, according to the present invention, the upper bottom surface of each section is continuously depressed at the base of the partition wall and the inner wall surrounding it, and a non-grid-like projection and / or depression is provided on the bottom surface. This is the fractionation medium used.
【０００６】更に、本発明は、培地の高さが隔壁のそれ
よりも高い前記分画培地である。Further, the present invention is the above-mentioned fractionation medium, wherein the height of the medium is higher than that of the partition walls.
【０００７】また、本発明は、容器が円形である前記分
画培地である。Further, the present invention is the above-mentioned fractionation medium, wherein the container is circular.
【０００８】更に、前記分画培地を製造する方法であ
る。Further, there is provided a method for producing the fractionation medium.
【０００９】[0009]
【発明の実施の態様】本発明の分画培地は、どのような
形状であってもよく、例えば円形、四角形のような形状
が挙げられる。このようなものとして、シャーレや培養
ビン等が挙げられる。また、分画培地の大きさも特に限
定されず、例えば分画培地がシャーレである場合、最も
スタンダードである９cm径のもののみならず、１３cm径
という大型のものであってもよい。DESCRIPTION OF THE PREFERRED EMBODIMENTS The fractionation medium of the present invention may have any shape, for example, a circular or square shape. Such things include petri dishes and culture bottles. The size of the fractionation medium is not particularly limited. For example, when the fractionation medium is a petri dish, it may be not only the most standard one having a diameter of 9 cm but also a large one having a diameter of 13 cm.
【００１０】本発明の分画培地は、その上底面に隔壁を
有し、隔壁と内側壁と上底面により囲まれた、複数の区
画が形成されている。区画の数は特に限定されない。ま
た、隔壁と側壁及び底面とは一体化していてもよく、あ
るいは、隔壁が上底面から分離するように構成してもよ
い。[0010] The fractionation medium of the present invention has a partition on the upper bottom surface, and has a plurality of compartments surrounded by the partition, the inner wall, and the upper bottom surface. The number of sections is not particularly limited. Further, the partition wall and the side wall and the bottom surface may be integrated, or the partition wall may be separated from the upper bottom surface.
【００１１】各区画内の上底面は、それを囲む隔壁及び
内側壁の根元で連続的に窪んでいる。このような、連続
的な窪み部が隔壁及び内側壁の根元に設けられた分画培
地は、驚くべきことに、窪み部が局部的（非連続的）に
設けられた分画培地、並びに突出部が連続的及び非連続
的に設けられた分画培地と比較し、著しく培地が剥離し
にくいことが判明した。窪み部の断面形状は特に限定さ
れず、例えば、凹状、Ｖ字状、Ｕ字状等の形状が挙げら
れる。隔壁の高さ（上底面を基準とした隔壁の高さ）に
対する、隔壁及び側壁の根元に設けられた窪み部の深さ
（上底面を基準とした窪み部の深さ）の比は、１／１０
〜１／２であるのが好ましく、１／９〜１／３であるの
がより好ましく、１／８〜１／４であるのが特に好まし
い。The upper bottom surface in each section is continuously depressed at the base of the partition wall and the inner wall surrounding it. Such a fractionation medium in which continuous depressions are provided at the base of the partition wall and the inner wall is, surprisingly, a fractionation medium in which depressions are provided locally (non-continuously), as well as a projection medium. It was found that the culture medium was remarkably hard to peel off as compared with the fractionation medium in which the portions were provided continuously and discontinuously. The cross-sectional shape of the concave portion is not particularly limited, and examples thereof include a concave shape, a V shape, and a U shape. The ratio of the depth of the recess provided at the root of the partition and the side wall (the depth of the recess based on the upper bottom) to the height of the partition (the height of the partition based on the upper bottom) is 1 / 10
The ratio is preferably from １ ／ to ２, more preferably from 1/9 to ３, and particularly preferably from ８ to ４.
【００１２】底面は平坦であるか、あるいは、底面に非
格子状の突起物及び／又は窪み部が設けられている。こ
こで、非格子状の突起物としては、例えば、針状突起
物、柱状の突起物、帯状突起物が挙げられる。The bottom surface is flat, or the bottom surface is provided with non-lattice-like projections and / or depressions. Here, examples of the non-lattice-shaped projection include a needle-shaped projection, a columnar projection, and a band-shaped projection.
【００１３】本発明の分画培地の容器は、周知の方法に
従い、例えば射出成形により一体成形的に製造しうる。
その後、同一種類又は異なった種類の液体状の培地を各
区画に注入する。その後冷却すると、やがて液体状の培
地はゲル化し、本発明の分画培地を得ることができる。
なお、液体状の培地を、米国特許３，９０２，９７２号
明細書及び特開平７−３０３４７０号公報記載の分画培
地のように、隔壁より高くかつ溢れ出ないように各区画
に注入することにより、固化した培地は、隔壁よりも高
くなるため、隔壁に引っかかることなく一度に試料を複
数の培地に塗布することができるようになる。[0013] The container of the fractionation medium of the present invention can be manufactured integrally by a known method, for example, by injection molding.
Thereafter, the same or different types of liquid culture media are injected into each compartment. After cooling, the liquid medium eventually gels, and the fractionation medium of the present invention can be obtained.
In addition, as in the fractionation medium described in U.S. Pat. No. 3,902,972 and JP-A-7-303470, a liquid medium is injected into each compartment so as to be higher than the partition wall and not to overflow. Accordingly, the solidified medium becomes higher than the partition walls, so that the sample can be applied to a plurality of mediums at once without being caught on the partition walls.
【００１４】[0014]
【発明の効果】本発明の分画培地は、十分な剥離防止機
能を有しており、例えば１３cm径の大型シャーレであっ
てもその効果は十分である。The fractionation medium of the present invention has a sufficient anti-peeling function, and its effect is sufficient even for a large petri dish with a diameter of, for example, 13 cm.
【００１５】[0015]
【実施例】実施例１ 射出成形により一体成形した、図１〜４に示される分画
シャーレ｛外径１３cm；隔壁３の高さ３mm；窪み部５の
幅２mm（隔壁根元）、２．５mm（側壁根元）；窪み部５
の深さ０．５mm（隔壁根元）、０．６mm（側壁根元）｝
中に、液体状の寒天培地２を注入し、続いて冷却して培
地をゲル化させた。その後、この分画シャーレ１を５℃
で３０日間放置して、培地２が隔壁３や側壁４から剥離
するか否か試験した。その結果、ゲル化や放置後に培地
の剥離は確認されなかった。EXAMPLES Example 1 The fractionation dish shown in FIGS. 1 to 4 integrally molded by injection molding: outer diameter 13 cm; height of partition 3 3 mm; width of recess 5 2 mm (root of partition), 2.5 mm (The base of the side wall);
0.5mm depth (base of partition wall), 0.6mm (root of side wall)
The liquid agar medium 2 was poured into the medium, followed by cooling to gel the medium. Then, this fractionated petri dish 1 was heated at 5 ° C.
For 30 days, and it was tested whether or not the culture medium 2 was separated from the partition walls 3 and the side walls 4. As a result, no detachment of the medium was observed after gelation or standing.
【図１】実施例１に係る分画シャーレの上面図である。FIG. 1 is a top view of a fractionating petri dish according to a first embodiment.
【図２】実施例１に係る分画シャーレの断面図（図１中
のＸ−Ｘ断面）である。FIG. 2 is a cross-sectional view (XX cross section in FIG. 1) of the fractionation petri dish according to Example 1.
【図３】実施例１に係る分画シャーレの隔壁部の拡大図
である（図１中のＸ−Ｘ断面）。FIG. 3 is an enlarged view of a partition wall portion of the fractionation petri dish according to the first embodiment (cross section taken along line XX in FIG. 1).
【図４】実施例１に係る分画シャーレの側壁部の拡大図
である（図１中のＸ−Ｘ断面）。FIG. 4 is an enlarged view of a side wall portion of the fractionation petri dish according to the first embodiment (cross section taken along line XX in FIG. 1).
１ 分画シャーレ ２ 寒天培地 ３ 隔壁 ４ 側壁 ５ 窪み部 ６ 蓋 ７ 上底面 1 Fractional Petri dish 2 Agar medium 3 Partition wall 4 Side wall 5 Depression 6 Cover 7 Upper bottom
───────────────────────────────────────────────────── フロントページの続き (72)発明者 宮尾 均 東京都中央区日本橋本町３丁目１番１号 極東製薬工業株式会社内 Ｆターム(参考） 4B029 AA08 AA27 BB02 BB06 CC02 GA01 GA02 GA08 GB04 GB10 4B063 QQ06 QQ07 QR69 QR82 4B065 AA01X AA57X BC31 BC46 CA46 CA60 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Hitoshi Miyao 3-1-1 Nihonbashi-Honcho, Chuo-ku, Tokyo F-term in Far East Pharmaceutical Co., Ltd. 4B029 AA08 AA27 BB02 BB06 CC02 GA01 GA02 GA08 GB04 GB10 4B063 QQ06 QQ07 QR69 QR82 4B065 AA01X AA57X BC31 BC46 CA46 CA60
Claims (5)
内側壁の根元で連続的に窪んでおり、かつ該底面が平坦
である分画培地。1. A fractionation medium in which the upper and lower surfaces of each compartment are continuously recessed at the base of a partition wall and an inner wall surrounding the compartment, and the bottom surface is flat.
内側壁の根元で連続的に窪んでおり、かつ該底面に非格
子状の突起物及び／又は窪み部が設けられている分画培
地。2. An upper surface of each partition is continuously recessed at the base of a partition wall and an inner wall surrounding the partition, and a non-lattice-like projection and / or recess is provided on the bottom surface. Fractionation medium.
求項１又は２記載の分画培地。3. The fractionation medium according to claim 1, wherein the height of the medium is higher than that of the septum.
れか一項記載の分画培地。4. The fractionation medium according to claim 1, wherein the container is circular.
培地を製造する方法。5. A method for producing the fractionation medium according to any one of claims 1 to 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28657098A JP4204674B2 (en) | 1998-09-30 | 1998-10-08 | Fractionation medium |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10-276986 | 1998-09-30 | ||
| JP27698698 | 1998-09-30 | ||
| JP28657098A JP4204674B2 (en) | 1998-09-30 | 1998-10-08 | Fractionation medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000166537A true JP2000166537A (en) | 2000-06-20 |
| JP4204674B2 JP4204674B2 (en) | 2009-01-07 |
Family
ID=26552201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP28657098A Expired - Lifetime JP4204674B2 (en) | 1998-09-30 | 1998-10-08 | Fractionation medium |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4204674B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001309777A (en) * | 2000-05-02 | 2001-11-06 | Nikken Seibutsu Igaku Kenkyusho:Kk | Divided medium for microorganism test and petri dish for making divided medium |
| JP2010527582A (en) * | 2007-05-30 | 2010-08-19 | 株式会社ニコン | Culture vessel |
| JP2011182648A (en) * | 2010-03-04 | 2011-09-22 | Dainippon Printing Co Ltd | Petri dish for making divided culture medium |
| JP2011211990A (en) * | 2010-04-01 | 2011-10-27 | Dainippon Printing Co Ltd | Petri dish for culture medium preparation |
-
1998
- 1998-10-08 JP JP28657098A patent/JP4204674B2/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001309777A (en) * | 2000-05-02 | 2001-11-06 | Nikken Seibutsu Igaku Kenkyusho:Kk | Divided medium for microorganism test and petri dish for making divided medium |
| JP2010527582A (en) * | 2007-05-30 | 2010-08-19 | 株式会社ニコン | Culture vessel |
| US8507261B2 (en) | 2007-05-30 | 2013-08-13 | Nikon Corporation | Incubation container |
| JP2011182648A (en) * | 2010-03-04 | 2011-09-22 | Dainippon Printing Co Ltd | Petri dish for making divided culture medium |
| JP2011211990A (en) * | 2010-04-01 | 2011-10-27 | Dainippon Printing Co Ltd | Petri dish for culture medium preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4204674B2 (en) | 2009-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6098802A (en) | Deep well rack assembly for pipette tips and the like | |
| US5534227A (en) | Thermoform dish insert | |
| JPH02500083A (en) | tissue culture vessels | |
| US20050151050A1 (en) | Ice cube tray | |
| US4380293A (en) | Socket wrench display package | |
| KR100322364B1 (en) | Pastry cutter set with combined storage case and support | |
| US5795775A (en) | Culture vessel and assembly | |
| JP2000166537A (en) | Divided culture medium | |
| US3799493A (en) | Mold for congealable foodstuffs and the like | |
| US3700204A (en) | Mold for ingestible substances | |
| US1921256A (en) | Multicompartment tray and method of making the same | |
| GB2113074A (en) | Container | |
| US6212793B1 (en) | Apparatus and method for drying and storing laboratory containers | |
| JP5682121B2 (en) | Petri dish for preparation of fractionation medium | |
| JP2005132406A6 (en) | Injection-molded plastic container or sealing device having a folded edge at the bottom and injection molding methods thereof | |
| JPH07303470A (en) | Fractionation medium and its production | |
| USD496145S1 (en) | Cap having embedded cooling system with convective/conductive coolant tubes and removable gel pack | |
| JP2567564Y2 (en) | Culture equipment | |
| US2564235A (en) | Ice tray | |
| JPH01254583A (en) | Package for confectionery | |
| JPH1080268A (en) | Petri dish having protrusions on inner circumference wall and its production | |
| JPH037323Y2 (en) | ||
| JP2003322441A (en) | Ice cube tray | |
| JP2627142C (en) | ||
| JP3004956U (en) | Fresh soap |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20050929 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20081007 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20081015 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20111024 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20111024 Year of fee payment: 3 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20121024 Year of fee payment: 4 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20121024 Year of fee payment: 4 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20131024 Year of fee payment: 5 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |