JP2000166534A - Fixing plate for sample chip for sample monitoring device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a fixing plate for a sample chip for a sample monitoring device capable of (1) surely preventing mixing of respective samples to be separately dispensed and surely monitoring respective sample, (2) enabling separately pouring various samples in high resolving power to make the monitoring work efficient and (3) simplifying a transferring mechanism of a separately dispensing needle separately dispensing various samples to make the process at a low cost. SOLUTION: This fixing plate 3 for a sample chip 1 for a sample monitoring device has a hydrophobic covering film 5 having prescribed breadth and thickness at a prescribed interval in a lattice-like and forms a hydrophilic covering film 9 in every cells 7 divided by each hydrophobic covering film 5.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plate for fixing a sample chip for a sample monitoring device used for adsorbing and immobilizing thousands of various kinds of samples including genes such as DNA and oligonucleotides to express and search the samples. About.

[0002]

For example, when expressing and searching for the various samples described above using a fluorescence microscope or the like, a sample chip in which thousands of types of samples are adsorbed and fixed at minute intervals on a fixed plate is used. As the sample chip, a large number of hydrophilic coatings having a molecular structure having a bond to a sample molecule such as polylysine are applied to the surface of a fixed plate such as a glass plate or a polyimide resin plate. It is prepared by coating cells with a film thickness of 10 to 10 μm to form cells, and adsorbing and fixing the sample solution to the hydrophilic film of each cell.

When manufacturing the sample chip, the tip of each dispensing needle in an integrated dispenser in which a plurality of dispensing needles are arranged at predetermined intervals (for example, 200 μm) is attached to each cell on the surface of the fixed plate. Although the sample solution stored in each dispensing needle is adsorbed by contact, each sample solution dispensed on the fixed plate may spread and mix with each other due to the hydrophilic action of the hydrophilic coating. Was. In this case, each sample could not be accurately monitored.

[0004] In addition, several thousand kinds of sample solutions are dispensed by controlling the movement of the integrated dispensing body in the row direction and the column direction so that the dispensing needle corresponding to each cell in the fixed plate is opposed. In order to prevent the sample solutions to be fixed from being mixed with each other while keeping the distance between them fixed, the integrated dispensing body is controlled with high precision so that each dispensing needle is positioned at the center of each cell. I needed to. For this reason, it is necessary to increase the precision of the moving mechanism of the integrated dispensing needle body, and there is a problem that the cost for producing the sample chip increases.

In order to monitor thousands of samples efficiently, it is necessary to increase the number of sample solutions to be dispensed on a fixed plate as much as possible. Because of the necessity of prevention, it is necessary to secure a certain interval between the samples, and there is a limit to the high resolution dispensing of the samples.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned conventional drawbacks. The object of the present invention is to surely prevent the dispensed samples from mixing with each other. It is an object of the present invention to provide a sample chip fixing plate for a sample monitoring device which enables accurate monitoring of a sample.

Another object of the present invention is to provide a sample chip fixing plate for a sample monitoring device, which can dispense various kinds of samples at a high resolution to increase the efficiency of a monitoring operation.

Another object of the present invention is to provide a sample chip fixing plate for a sample monitoring device, which can simplify a moving mechanism of a dispensing needle for dispensing various samples and reduce the cost. It is in.

[0009]

SUMMARY OF THE INVENTION Accordingly, the present invention provides a sample chip for a sample monitoring device that expresses and searches for various samples dispensed on a fixed plate. A hydrophilic film is formed in a grid at predetermined intervals and a hydrophilic film is formed in each cell defined by each hydrophobic film.

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing a fixing plate of a sample chip for a sample monitoring device. FIG. 2 is II of FIG.
FIG. 2 is an enlarged vertical sectional view taken along line II.

The fixed plate 3 of the sample chip 1 is made of a glass plate or a polyimide resin plate having a predetermined thickness (about 2 to 3 mm), and the surface of the fixed plate 3 has a predetermined width (for example, the interval between the cells 7 is 200 μm). About 30μ
m) The hydrophobic coatings 5 are coated in a lattice shape with a mutual interval of about 200 μm. And each hydrophobic coating 5
Each cell 7 partitioned by each other is formed in a size of about 70 × 70 μm.

The hydrophobic film 5 is made of a polyfluoroethylene resin (trade name: Teflon, manufactured by DuPont), a silicon resin or a gold thin film, and is formed by a printing method, a photolithography method, an etching method, or the like.

Each cell 7 partitioned by each hydrophobic coating 5
Inside, a hydrophilic coating 9 having a thickness substantially equal to that of the hydrophobic coating 5 is formed. For example, the sample is D
In the case of NA, the cell 7 is composed of polylysine having a molecular arrangement having a bond to DNA, and the hydrophilic coating 9 is filled in each cell 7 by a dipping method, a flow coating method or the like.

The hydrophilic coating 9 is appropriately selected depending on the sample to be adsorbed and fixed on the sample chip 1. In any case, the hydrophilic coating 9 has a molecular arrangement having a bond to the sample molecule. Is desirable. When the fixing plate 3 coated with the hydrophobic coating 5 is immersed in the hydrophilic coating 9 solution to fill the cell 7 with the hydrophilic coating 9, or when the surface of the fixing plate 3 is coated with the hydrophilic coating 9 solution, When the cell 7 is filled with the hydrophilic film 9 to form the hydrophilic film 9, the excess hydrophilic film 9 solution adhered on the hydrophobic film 5 is scraped off by the scraper or the like because the hydrophobic film 5 itself is hydrophobic. , And can be easily and completely removed.
In addition, examples of the sample monitoring device (not shown) include a fluorescence microscope, a surface plasmon resonance angle detection device, and the like.

Next, the action of dispensing and adsorbing the sample solution to the fixed plate 3 will be described. FIG. 3 is an enlarged vertical sectional view showing an example of sample adsorption. FIG. 4 is an enlarged vertical sectional view showing an example of sample adsorption.

First, a plurality of, for example, six dispensing needles 13 for dispensing various samples such as genes and proteins such as DNA and oligonucleotides into each cell 7 of the fixed plate 3 are attached to each cell 7. 7 are stacked in a line at an interval corresponding to the mutual interval of 7, and the tip of each dispensing needle 13 is formed to be about 50 μm. A reservoir 13a such as a vertically split groove is formed at the tip of each dispensing needle 13, and a sample solution to be dispensed in the reservoir 13a is stored in advance.

After the tip of each dispensing needle 13 is opposed to the center of each cell 7 by controlling the movement of the accumulation dispensing body 12 in the XY axis direction, the accumulation dispensing body 12 is moved in the Z axis direction. When the tip of each dispensing needle 13 is moved and pressed against the hydrophilic coating 9 in each cell 7, the sample solution is dispensed by its surface tension. At this time, since the hydrophilic coating 9 itself is hydrophilic, the dispensed sample solution binds to each molecule of the hydrophilic coating 9 and spreads throughout the cell 7 to be adsorbed at a substantially uniform concentration.

Each of the sample solutions adsorbed on the hydrophilic coating 9 in each cell 7 oozes out from the hydrophilic coating 9 because the cells 7 are partitioned by the hydrophobic coating 5. Mixing with the sample solution adsorbed in the cell 7 is prevented.

On the other hand, even when the tip of each dispensing needle 13 of the accumulating dispensing body 12 is dispensed in a state where it is displaced from the center of each of the cells 7, the pressure welding displaced from the center of each cell 7 is performed. It is almost uniformly spread and adsorbed throughout the cell 7 around the location.

The sample solution adsorbed on the hydrophilic coating 9 in each cell 7 by the above-mentioned operation is dried and fixed to produce the sample chip 1.

In the present embodiment, the hydrophilic coating 9 of each cell 7 is partitioned by the hydrophobic coating 5 to prevent the sample solution adsorbed on each cell 7 from oozing and mixing.
Also, even if each dispensing needle 13 of the integrated dispensing body 12 is displaced from the center of the cell 7, the sample solution is spread over the whole cell and absorbed by the hydrophilic action of the hydrophilic film 9. Can be. Furthermore, since each cell 7 can be reliably partitioned by the hydrophobic film 5, the interval between the cells 7 can be narrowed, and a large number of sample solutions can be dispensed.

[0022]

As described above, the present invention makes it possible to prevent each sample to be dispensed from being mixed with each other and to accurately monitor each sample. Further, according to the present invention, monitoring work can be made more efficient by dispensing various types of samples with high resolution. Further, according to the present invention, the moving mechanism of the dispensing needle for dispensing various samples can be simplified and the cost can be reduced.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a fixing plate of a sample chip for a sample monitoring device.

FIG. 2 is an enlarged vertical sectional view taken along the line II-II of FIG.

FIG. 3 is an enlarged vertical sectional view showing an example of sample adsorption.

FIG. 4 is an enlarged vertical sectional view showing an example of sample adsorption.

[Explanation of symbols]

1-sample chip, 3-fixed plate, 5-hydrophobic coating,
7-cell, 9-hydrophilic film, 12-dispensed dispenser, 13-
Dispensing needle

 ──────────────────────────────────────────────────の Continued on the front page F term (reference) 4B024 AA11 AA20 CA01 CA11 HA11 4B029 AA08 AA23 BB15 BB20 CC02 CC08 GA03 GA08 GB04 GB05 GB09 GB10

Claims (4)

[Claims] In a sample chip for a sample monitoring device for expressing and searching various samples dispensed on a fixed plate, a hydrophobic film having a predetermined width and a predetermined thickness is formed on the fixed plate in a grid pattern at predetermined intervals. A plate for fixing a sample chip for a sample monitoring device, wherein a hydrophilic coating is formed in each cell partitioned by each hydrophobic coating. 2. A plate for fixing a sample chip for a sample monitoring device according to claim 1, wherein the hydrophobic film is made of a fluorinated resin and the hydrophilic film is made of a molecular structure having bonds to sample molecules. 3. The plate according to claim 1, wherein the hydrophobic coating is made of a silicone resin. 4. A plate for fixing a sample chip for a sample monitoring device according to claim 1, wherein the hydrophobic coating is made of a gold thin film.