JP2000143520A - Growth promoter of enteric bifidus bacillus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject promoter capable of being not assimilated by enteric deleterious bacteria and opportunistic bacteria but assimilated by only Bacillus bifidus of most effective bacteria in high selectivity, and promoting the growth of the enteric Bacillus bifidus by using ghatti gum as an active ingredient. SOLUTION: This promoter contains ghatti gum as an active ingredient. The ghatti gum is branched polysaccharides composed of L-arabinose, D- galactose, D-mannose, D-xylose and D-glucuronic acid, and the content thereof is 0.1-20 wt.%. Vitamins such as pantothenic acid and riboflavin can be used in combination with the ghatti gum, and other vegetable fibers, oligosaccharides, minerals and the like can be formulated therewith. The daily dose is about 0.1-10 g, and the dose is preferably administered by dividing the dose into one to several times.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to the promotion of intestinal bifidobacteria growth, which is not assimilated to intestinal harmful bacteria and opportunistic bacteria but is highly selectively assimilated to the most useful bacteria, bifidobacteria. Agent.

[0002]

BACKGROUND OF THE INVENTION Bifidobacteriaceae 1) normalizes the balance of the intestinal flora and protects the body from intestinal infections and food poisoning caused by pathogenic bacteria, and 2) suppresses intestinal spoilage. To clean the intestines,
It is one of the most useful bacteria in the intestine that exhibits excellent effects such as preventing diseases and aging such as diarrhea, constipation, developmental disorders, cancer, high blood pressure, and gastrointestinal disorders. Oligosaccharides such as raffinose and isomaltoligosaccharide are known as factors for growing such bifidobacteria in the intestine (JP-A-61-2).
27777, etc.). However, although such oligosaccharides are assimilated by bifidobacteria, there is a problem that harmful bacteria such as Clostridium perfringens and opportunistic bacteria such as Bacteroides vulgatus are also utilized. On the other hand, polysaccharides such as xanthan gum and other dietary fibers have a problem that they are hardly assimilated by harmful bacteria or opportunistic bacteria, but hardly assimilated by bifidobacteria.

For this reason, harmful bacteria and opportunistic bacteria are not utilized,
There has been a demand for a substance that is highly selectively assimilated only to bifidobacteria and promotes the growth of intestinal bifidobacteria.

[0004]

DISCLOSURE OF THE INVENTION The present inventors have studied the assimilation of intestinal bacteria such as a large number of oligosaccharides, natural product extracts, dietary fiber, and polysaccharides. It was found that it was not assimilated at all to opportunistic bacteria, but was selectively assimilated only to bifidobacteria to promote the growth of intestinal bifidobacteria. That is, the present invention provides an intestinal bifidobacterium growth promoter comprising gutti gum as an active ingredient.

[0005]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Guttie gum is a branched polysaccharide composed of L-arabinose, D-galactose, D-mannose, D-xylose and D-glucuronic acid. It has been used as a buffer since it has an effect. However, it was not known at all that gutti gum could highly selectively grow bifidobacteria without growing intestinal harmful bacteria and opportunistic bacteria.

As an intestinal bifidobacterium growth promoter used in the present invention, gutti gum may be used as it is, but vitamins such as pantothenic acid and riboflavin are essential as growth factors for intestinal bifidobacteria. Then it is more effective. Further, other dietary fibers, oligosaccharides, minerals and the like can be blended as long as the effects of the present invention are not impaired.

The intestinal bifidobacterium growth promoter of the present invention comprises:
Bifidobacterium longum (Bif. Longum), Bifidobacterium bifidum (Bif. Bifidum), Bifidobacterium breve (Bif. Breve), etc., to promote the growth of any Bifidobacterium resident in the human intestine Although effective, it is particularly effective against Bifidobacterium longum.

[0008] The intestinal bifidobacterium growth promoter of the present invention may be in any form, but is preferably in the form of, for example, a drug, food or drink. When used as a pharmaceutical, in addition to gutti gum, if necessary, the above vitamins, minerals, suspending agents, emulsifiers, stabilizers, excipients, binders, disintegrants, lubricants, flavoring agents, storage Agent, coloring agent, fragrance, etc. as appropriate,
For example, it can be made into a form such as a sugar coating according to a conventional method. In addition, when used as food or drink, in addition to gutti gum, the above vitamins, minerals, coloring agents, flavors, and the like are appropriately blended as necessary, and in a conventional manner, for example, into a form of confectionery, soft drink, fermented milk, or the like. be able to.

The content of the gutti gum in the intestinal bifidobacterium growth promoter of the present invention is from 0.1 to 20% by weight (hereinafter simply referred to as%), particularly from 0. 1-10% is preferred. When used as a pharmaceutical, 1 to 20%, particularly 1 to 10% is preferable. When used as food or drink, 0.1 to 6%, particularly 0.1 to 3%.
% Is preferred.

The daily intake of gutti gum is not constant depending on age, body weight, symptoms, etc., but is preferably 0.1 to 10 g, particularly 0.5 to 7 g, from the viewpoint of effect and economy. It is preferable to take it once or several times a day.

The intestinal bifidobacterium growth promoter of the present invention comprises:
Although effective in promoting the growth of human intestinal bifidobacteria, it can also be used to promote the growth of intestinal bifidobacteria in livestock such as pigs, cattle and sheep.

[0012]

Example 1 Test Example 1 Bifidobacterium, Harmful Bacteria and Opportunistic Bacterium assimilation Test Bifidobacterium longum as Bifidobacterium
JCM1217 strain, Clostridium perfringens JCM1290 strain as a harmful bacterium, and Bacteroides burgatas JCM5826 strain as an opportunistic bacterium were used. PYF medium containing oligosaccharide, dietary fiber, natural product extract, polysaccharide, and glucose as a positive control (0.5% each) shown in Table 1 (Peptide
on Yeast extract Fildes solution) with 10 ⁷ c. f. Each was inoculated independently so as to obtain u. These were anaerobically cultured at 37 ° C. for 3 days, and the turbidity (OD 600 nm) and pH of the culture solution were measured. Table 1 shows the results.

[0013]

[Table 1]

Guttie gum is not assimilated to harmful bacteria or opportunistic bacteria at all, but is assimilated only to bifidobacteria.
H decreased. That is, Guttigum promotes the growth of bifidobacteria, suppresses the growth of harmful bacteria, opportunistic bacteria,
It has been shown that the intestinal environment can be improved. Oligosaccharides and glucose were assimilated into any of bifidobacteria, harmful bacteria, and opportunistic bacteria. Polysaccharides and dietary fiber other than gutti gum were either not assimilated by any of bifidobacteria, harmful bacteria, or opportunistic bacteria, or were assimilated by harmful bacteria or opportunistic bacteria. In other words, only assimilated by bifidobacteria,
The only thing that could not be assimilated by harmful bacteria or opportunistic bacteria was gutti gum.

Test Example 2 Bifidobacterium Proliferation Test Using Wistar Rats Fifteen 3-week-old Wistar rats were divided into three groups of five, and the first group received a standard feed (manufactured by Oriental Yeast).
To the second group, a feed obtained by adding 5% of guttie gum to the standard feed was continuously administered for one week. After completion of the administration, the cecal contents of each Wistar rat were collected, diluted with physiological saline, spread on various intestinal bacterial selection media, and cultured according to the method of Mitsuoka et al. The number of bifidobacteria (the number of bacteria per gram of cecal contents) was measured. In the third group, the cecal contents were immediately collected without any administration, and the number of bifidobacteria was similarly measured (control). The average value of 5 animals in each group was 4.2 × 10 ⁷ for the first group and 5.2 × 1 for the second group.
0 ⁸ , and 4.6 × 10 ⁷ for the third group. From this, it has been clarified that intestinal bifidobacteria do not grow even after ingestion of the standard feed for one week, but intestinal bifidobacteria increase when gutti gum is consumed for one week.

Examples 1 and 2 Beverages and tablets were prepared according to the conventional methods with the formulations shown in Tables 2 and 3. These showed excellent bifidobacterium growth promotion effects.

[0017]

[Table 2] Guttie gum 0.5 g Vitamin C 0.3 g Sugars (sugar, fructose-glucose liquid sugar) 10 g Fragrance 0.025 g Distilled water Balance 100 ml

[0018]

Table 3 Guttie gum 10 g Excipient 40 g Flavor 0.1 g Lubricant 2 g Erythritol 47.9 g Total 100 g

[0019]

The use of the intestinal bifidobacterium growth promoter of the present invention can suppress the growth of intestinal harmful bacteria and opportunistic bacteria and promote the growth of bifidobacteria.

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Claims (1)

[Claims] 1. An intestinal bifidobacterium growth promoter comprising gutti gum as an active ingredient.