JP2000093834A - Magnetic stand - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a magnetic stand to which containers such as micro-tubes can be effectively fixed and used for separating magnetic powder suspended in liquid in the containers without cost needed. SOLUTION: In a magnetic stand provided with a rear plate 1 having a semi-cylindrical or semi-conic groove, permanent magnets buried in the rear of the groove and parallel to the groove and a bottom plate 4 vertically arranged below the rear plate 1, it is provided the front face of the rear plate 1 with semi-elliptic arms 5 each consisting of elastic materials oppositely to each other arranged in the horizontal direction so that the groove is put between them and so that they swell forward.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a magnetic stand for separating magnetic powder suspended in a liquid in a container. In particular, the present invention relates to a magnetic stand useful in the field of biochemistry and molecular biology for separating biological components such as nucleic acids, proteins or cells from biological materials and the like using magnetic particle powder as a carrier.

[0002]

2. Description of the Related Art At present, a method of separating a biomaterial supplemented by a magnetic powder carrier using a magnetic force has been widely used in the fields of biochemistry and molecular biology. The background is the complexity of the centrifugation operation. Stands for microtubes and test tubes provided with permanent magnets for separating biological components using a magnetic powder carrier have been devised and are now widely used. However, at present, most of them have insufficient or no mechanism for bringing the container into close contact with the magnet on the back surface.

[0003] If the container is not tightly attached to the magnet,
When the cap of the microtube or the lid of the test tube is opened, the fixation of the container becomes unstable, the liquid often splatters, and the adjacent container or finger is often contaminated. Also, when draining from the container by decantation,
The container must be completely adhered to the back.

For example, in WO 90/14891, a method has been devised in which a flat polystyrene resin or the like is arranged in a vertical direction and the elasticity of a plate is used. However, in this method, it is difficult to make the materials of the main body and the plate uniform, and there is a disadvantage that the cost of manufacturing the stand is high since it cannot be integrally formed. Further, there is a need for improvement in that it is difficult to clean the stand when contamination occurs. Further, generally, when such a mechanism is used, the holding force tends to be weak.

[0005]

As described above, the most important issues are to establish a method for efficiently fixing a container such as a microtube and a method for manufacturing a stand that does not require cost.

[0006]

Means for Solving the Problems In order to solve the above problems, the present inventors have studied a method for efficiently fixing a container. As a result, the container can be efficiently fixed to the magnet surface of the stand by using a resilient arcuate arm that sandwiches the semi-cylindrical groove on the back as a mechanism for pressing the container to the back. This led to the completion of the present invention.

That is, the present invention provides a back plate having a semi-cylindrical or semi-conical groove, a permanent magnet embedded parallel to the back of the groove, and a bottom plate vertically disposed below the back plate. A magnetic stand comprising: a bow-shaped arm made of an elastic material facing each other and arranged in the horizontal axis direction so as to sandwich the groove on the front surface of the back plate and to bulge forward. A magnetic stand characterized by the above.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The arms of the present invention include nylon, polyethylene, polypropylene, polystyrene,
An elastic material such as a resin such as acrylic resin, fluororesin, polycarbonate, methinepentene resin, polyurethane, ABS, and periprene, or a metal is used. Among them, it is particularly preferable to use a nylon resin from the viewpoint of ease of processing. When a metal is used, stainless steel, nickel, aluminum and the like are preferably used. By making the inner diameter of the circle formed by the arcuate arm and the groove on the back slightly smaller than that of a container that slightly fixes the container, it is possible to obtain a sufficient pressing force to hold the container. The thickness of the arm is optimized according to the material used.

Further, by adding a small protrusion to the tip of the arm, it is possible to keep the pressing force substantially constant irrespective of the outer diameter of the container to be fixed. In such a case, the inner diameter of the arm is equal to or slightly larger than the outer diameter of the container, and the tip of the projection is designed to be inside the outer diameter of the container. Thus, since the protrusion comes into contact with the container at a point, the pressing force can be maintained at a substantially constant sufficient pressing force even when the outer diameter of the container slightly changes.

The arm in the present invention refers to a semicircular projection for fixing the container. In the present invention, it is preferable to use a pair of arms,
It is not always necessary to make a pair.

The pressing force sufficient to hold the container as described above means a force that does not cause the container to fall even if inverted.

On the other hand, by providing a small hole in the bottom plate corresponding to the vicinity of the midpoint of the semi-cylindrical groove, the stability of the fixed container can be drastically improved. Particularly, when opening and closing the lid of the container, the bottom of the tube serves as a fulcrum of the force. Therefore, when a container having a hard lid is used, the hole should be provided.

When the stand is actually manufactured, the arm, the back plate and the bottom plate are integrally formed, so that the assembling time and the manufacturing process can be saved. On this occasion,
By simultaneously forming the hole for embedding the plate magnet in the back plate, the step of incorporating the magnet can be greatly simplified.

The magnet used in the present invention includes:
Rare earth magnets such as strong neodymium magnets and cobalt magnets are suitable. It is also possible to use an electromagnet.

According to one aspect of the present invention, a pair of resilient, arcuate arms that sandwich a semi-cylindrical groove in the back plate are provided, and are integrally molded with a nylon resin that can accommodate 10 samples. And a magnetic stand for a 1.5 ml microtube.

[0016]

EXAMPLES The effects of the present invention will be further clarified by specifically describing the form of the stand according to the present invention in the examples.

FIGS. 1 and 2 show an embodiment of a magnetic stand according to the present invention, and show a magnetic stand compatible with five samples. As shown in FIGS. 1 and 2, a semi-cylindrical groove 2 is formed in a back plate 1, a groove 3 for embedding a magnet so as to be parallel to the back thereof, and a bottom of a test tube or a microtube can be fixed to a bottom plate 4. Hole 6 for
It was designed to have

Next, the above-mentioned structure was integrally formed with the same mold using nylon resin as a material. After molding, a plate-shaped neodymium magnet was embedded from above, and an adhesive was poured into the upper portion and fixed. The arm 5 was designed to be substantially the same as or slightly larger than the minor diameter of the ellipse formed by the groove and the arm or the diameter of the circle and the outer diameter of the test tube used. The protruding portion 7 at the tip of the arm was designed to be slightly inside the outer diameter of the container, and was designed to be in contact with the fixed container at a point.

FIG. 3 shows a possible preferred arm configuration. The shape of the arm is appropriately selected depending on the shape and size of the object to be fixed. As described above, the protruding portions at the ends of the arms 1 and 2 are for keeping the pressing force substantially constant even when the outer diameter of the container to be fixed is slightly changed. Further, in the case of strong fixing, a flat structure such as 3 is suitable. In such a case, the inner diameter of the arm is designed to be slightly smaller than the outer diameter of the container.

[0020]

As described above, the magnetic stand according to the present invention achieves efficient fixing of the container to the back of the stand and low cost. That is, compared to the conventional magnetic stand,
An object of the present invention is to provide a magnetic stand for separating magnetic fine particles which is inexpensive and easy to use.

[Brief description of the drawings]

FIG. 1 is a top view of one embodiment (for five samples) of a magnetic stand according to the present invention.

FIG. 2 is a side view of one embodiment (for 5 samples) of the magnetic stand according to the present invention.

FIG. 3 is a diagram showing an example of the shape of an arm in a magnetic stand according to the present invention.

[Explanation of symbols]

Reference Signs List 1 back plate 2 semi-cylindrical groove 3 groove for embedding magnet 4 bottom plate 5 arm 6 hole for fixing bottom of test tube or micro tube

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Yoshihisa Kawamura 10-24, Toyo-cho, Tsuruga-shi, Fukui F-term in Tsubo Bio-Laboratory, Toyobo Co., Ltd. 4B029 AA23 AA27 BB01 BB15 BB20 CC01

Claims (2)

[Claims] 1. A back plate having a semi-cylindrical or semi-conical groove, a permanent magnet embedded parallel to the back of the groove, and a bottom plate vertically disposed below the back plate. A magnetic stand, comprising: a bow-shaped arm made of an elastic material facing each other so as to sandwich the groove on the front surface of the back plate and to swell forward. Magnetic stand. 2. The arm according to claim 1, wherein the material of the arm is selected from the group consisting of nylon, polyethylene, polypropylene, polystyrene, acrylic resin, fluororesin, polycarbonate, methinepentene resin, polyurethane, ABS, periprene and metal. Magnetic stand.