JP2000010017A - Cover glass for microscopic observation and observation method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve the reproducibility of microscopic observation conditions without the occurrence of flawing in a sample, to prolong the life of the sample and to enable long-time observation with cover glass 1 to be put on a slide used for microscopic observation by forming a spacer region between the cover glass and slide glass as a structure partially large in its thickness. SOLUTION: The spacer region 2 between the cover glass 1 and the slide glass 3 is formed as the structure partially thick in its thickness. Further, a structure stuck with a film is used as the thickened portion and a tacky adhesive tape is adopted for the film stuck thereto. A sealant is inserted into the clearance between the peripheral of the cover glass 1 and the slide glass 3 and fluid paraffin is adopted for the sealant. An agar film 4 is placed on the slide glass 3 and the sample 5 is placed thereon. Further, the cover glass 1 having the spacer 2 consisting of the tacky adhesive tape, such as cellophane tape, bored with a hole evading the portion where the sample 5 exists is put thereon from above.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a cover glass for microscope observation and an observation method.

[0002]

2. Description of the Related Art Conventionally, when a living sample is observed alive under a microscope, the sample is placed on a slide glass together with a small amount of a buffer solution, and a small amount of a spacer such as petrolatum is applied to four sides of a cover glass and covered. Prevent the evaporation of water and prevent the sample from being pressurized, or place an agar film in an island shape on a slide glass, place the sample on the agar, and cover it directly with a cover glass to prepare an observation sample Was.

[0003]

However, in the conventional method using petrolatum, it is difficult to apply the four sides uniformly, and the distance between the cover glass and the sample is not constant. I could not do it. When an agar film was used, pressure from the cover glass was directly applied to the sample, and the sample was damaged. In addition, the moisture of the agar gradually evaporated during the observation, and the life of the sample was shortened by drying, so that observation for a long time was impossible.

It is an object of the present invention to provide a novel cover glass for microscope observation and an observation method which have solved the above-mentioned problems.

[0005]

The above object is achieved by the following means. That is, the present invention provides a cover glass that covers a slide used for microscopic observation, in which the spacer region between the slide glass and the slide glass has a structure in which the thickness is partially thick, and further, the film is formed as a thickened portion. The attached film is an adhesive tape, and the attached film is an adhesive tape, and provides a cover glass for microscopic observation having a spacer region except for a region corresponding to a visual field, or the cover glass is placed on a slide glass. The present invention provides a microscope observation method in which a sealant is inserted into a gap between the coverglass and a slide glass around the sample, and the sealant is liquid paraffin.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail.

FIG. 1 is a perspective view showing a procedure for preparing a microscope specimen, and FIG. 2 is a perspective view showing the entire structure of the microscope specimen.

In the present invention, as shown in FIG. 1, an agar film 4 is placed on a slide glass 3 and a sample 5 is placed thereon.
Is further covered with a cover glass 1 provided with a spacer 2 made of an adhesive tape such as scotch tape having a hole formed so as to avoid a portion of the visual field, that is, a portion where the sample 5 exists. Therefore, the weight of the cover glass 1 is applied to the agar film 4 with which the cover glass 1 is in contact, but the portion where the sample 5 is present does not have the spacer 2, so that the cover glass 1 and the sample 5 do not directly contact. That is, according to the present invention, the sample 5 is not weighted and no damage occurs. Further, a gap 7 between the cover glass 1 and the slide glass 3 around the cover glass 1.
Since the sealing agent is inserted into the sample, the drying of the agar film 4 can be suppressed, the life of the sample 5 can be extended, and long-term observation can be performed.

[0009]

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples.

(Example 1) Nematode Caenorhabdi
According to the present invention, a fertilized egg of tis elegans (hereinafter simply referred to as a nematode) is used as a microscope specimen, and Zeiss Ax
Embryo development was observed using an iophoto differential interference microscope and a 100 × oil immersion objective. 2.5 for agar film
% Agar (Difco) was used. Buffer solution contains potassium dihydrogen phosphate 3 g / l and sodium monohydrogen phosphate 6 g
/ L, sodium chloride 5 g / l, and 1 mM magnesium sulfate. Cover glass is 18x24m
m and a thickness of 0.15 mm were used. Scotch Mending Tape (Sumitomo 3M) for spacer
Was cut into 12 × 20 mm and a circular hole having a diameter of 5 mm was formed in the center. Liquid paraffin used was a first-class reagent of Wako Pure Chemical. FIGS. 1 and 2 show the procedure for preparing the microscope specimen and the entire structure of the microscope specimen, respectively. Eighty individuals were examined and after 15 hours hatching of all embryos was confirmed. Approximately 30% of the embryos did not hatch in the conventional method without the spacer and sealant.

(Example 2) When the cell division order of a nematode embryo was traced by the same method, the division of P3 cells occurred in all of the five individuals observed after AB cells were divided into 16 cells. This is a normal division order. In the conventional method, the cell division order was reversed in three of the six individuals observed. This is the result of the embryo being damaged by pressure.

[0012]

According to the present invention, since the cover glass and the sample are not in direct contact with each other, the sample is not weighted and the sample is not damaged. Therefore, a sample without damage can be observed. Further, since the distance from the cover glass to the sample is constant, the reproducibility of the microscope observation conditions is good. In addition, since the sealant is inserted into the gap between the cover glass and the slide glass, drying of the agar can be suppressed, the life of the sample can be extended, and long-term observation can be performed.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a procedure for preparing a microscope sample for showing an embodiment of the present invention.

FIG. 2 is an overall structure of a microscope specimen of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Cover glass 2 Spacer 3 Slide glass 4 Agar film 5 Sample 6 Buffer droplet 7 Gap between slide glass and cover glass

Claims (6)

[Claims] 1. A cover glass for microscope observation, wherein the cover glass used for microscope observation has a structure in which a spacer region between the slide glass and the slide glass has a partially thick structure. 2. The cover glass for microscopic observation according to claim 1, wherein the thick structure portion has a structure in which a film is attached. 3. The cover glass for microscopic observation according to claim 2, wherein the attached film is an adhesive tape. 4. The cover glass for microscope observation according to claim 1, which has a spacer region except for a region corresponding to a visual field. 5. In a microscope observation, the cover glass for microscope observation according to any one of claims 1 to 4 is placed over a sample on a slide glass, and a seal is provided in a gap between the cover glass and the slide glass. A microscopic observation method characterized by inserting an agent. 6. The microscope observation method according to claim 5, wherein the sealant is liquid paraffin.