ITRM20130648A1 - Metodo ed apparato per discriminare tra spermatozoi x e y basato sulla spettroscopia raman - Google Patents

Metodo ed apparato per discriminare tra spermatozoi x e y basato sulla spettroscopia raman

Info

Publication number
ITRM20130648A1
ITRM20130648A1 IT000648A ITRM20130648A ITRM20130648A1 IT RM20130648 A1 ITRM20130648 A1 IT RM20130648A1 IT 000648 A IT000648 A IT 000648A IT RM20130648 A ITRM20130648 A IT RM20130648A IT RM20130648 A1 ITRM20130648 A1 IT RM20130648A1
Authority
IT
Italy
Prior art keywords
spermatozoes
discriminating
raman spectroscopy
raman
spectroscopy
Prior art date
Application number
IT000648A
Other languages
English (en)
Inventor
Donatella Balduzzi
Giuseppe Coppola
Luca Anna Chiara De
Maria Antonietta Ferrara
Pietro Ferraro
Andrea Galli
Roberto Puglisi
Luigi Sirleto
Original Assignee
Consiglio Nazionale Ricerche
Istituto Sperimentale Italiano Lazz Aro Spallanzan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consiglio Nazionale Ricerche, Istituto Sperimentale Italiano Lazz Aro Spallanzan filed Critical Consiglio Nazionale Ricerche
Priority to IT000648A priority Critical patent/ITRM20130648A1/it
Priority to PCT/IB2014/066319 priority patent/WO2015075698A1/en
Priority to EP14815053.5A priority patent/EP3074750A1/en
Publication of ITRM20130648A1 publication Critical patent/ITRM20130648A1/it

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • G01N15/149—
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/32—Micromanipulators structurally combined with microscopes
IT000648A 2013-11-25 2013-11-25 Metodo ed apparato per discriminare tra spermatozoi x e y basato sulla spettroscopia raman ITRM20130648A1 (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
IT000648A ITRM20130648A1 (it) 2013-11-25 2013-11-25 Metodo ed apparato per discriminare tra spermatozoi x e y basato sulla spettroscopia raman
PCT/IB2014/066319 WO2015075698A1 (en) 2013-11-25 2014-11-25 Method and apparatus for discriminating x and y sperms based upon raman spectroscopy
EP14815053.5A EP3074750A1 (en) 2013-11-25 2014-11-25 Method and apparatus for discriminating x and y sperms based upon raman spectroscopy

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT000648A ITRM20130648A1 (it) 2013-11-25 2013-11-25 Metodo ed apparato per discriminare tra spermatozoi x e y basato sulla spettroscopia raman

Publications (1)

Publication Number Publication Date
ITRM20130648A1 true ITRM20130648A1 (it) 2015-05-26

Family

ID=49780243

Family Applications (1)

Application Number Title Priority Date Filing Date
IT000648A ITRM20130648A1 (it) 2013-11-25 2013-11-25 Metodo ed apparato per discriminare tra spermatozoi x e y basato sulla spettroscopia raman

Country Status (3)

Country Link
EP (1) EP3074750A1 (it)
IT (1) ITRM20130648A1 (it)
WO (1) WO2015075698A1 (it)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6396911B2 (ja) 2012-10-15 2018-09-26 ナノセレクト バイオメディカル， インコーポレイテッド 粒子を選別するためのシステム、装置、および、方法
CN109690294A (zh) * 2016-07-04 2019-04-26 塞尔图股份有限公司 用于测定转染的装置和方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004101323A (ja) * 2002-09-09 2004-04-02 Suruga Seiki Kk Ｘ・ｙ精子の選別方法及び装置
DE102007013107A1 (de) * 2007-03-15 2008-09-18 Friedrich-Schiller-Universität Jena Verfahren zur Bestimmung des Gschlechts von Vögeln
WO2009149733A1 (en) * 2008-06-13 2009-12-17 Embl Heidelberg Next generation flow cytometer sorter
DE102010023099B3 (de) * 2010-06-09 2011-11-17 Celltool Gmbh Verfahren und Vorrichtung zum Charakterisieren von biologischen Objekten
US20120225474A1 (en) * 2010-11-16 2012-09-06 1087 Systems, Inc. System and method for pathogen detection and identification

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5021244A (en) 1988-12-06 1991-06-04 Cytogam, Inc. Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004101323A (ja) * 2002-09-09 2004-04-02 Suruga Seiki Kk Ｘ・ｙ精子の選別方法及び装置
DE102007013107A1 (de) * 2007-03-15 2008-09-18 Friedrich-Schiller-Universität Jena Verfahren zur Bestimmung des Gschlechts von Vögeln
WO2009149733A1 (en) * 2008-06-13 2009-12-17 Embl Heidelberg Next generation flow cytometer sorter
DE102010023099B3 (de) * 2010-06-09 2011-11-17 Celltool Gmbh Verfahren und Vorrichtung zum Charakterisieren von biologischen Objekten
US20120225474A1 (en) * 2010-11-16 2012-09-06 1087 Systems, Inc. System and method for pathogen detection and identification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LAU ADRIAN Y ET AL: "An integrated optofluidic platform for Raman-activated cell sorting", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY - CAMBRIDGE, GB, vol. 8, 28 May 2008 (2008-05-28), pages 1116 - 1120, XP002519719, ISSN: 1473-0197, DOI: 10.1039/B803598A *

Also Published As

Publication number Publication date
EP3074750A1 (en) 2016-10-05
WO2015075698A1 (en) 2015-05-28

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