IT202100020249A1 - BLS3 mobile biotechnological laboratory with innovative dead-man emergency pass box - Google Patents

Description

BSL3ag mobile biotechnological laboratory with rapid evacuation

DESCRIPTION

of the invention having the TITLE: ?BSL3-ag classified mobile biotechnological laboratory with rapid evacuation?,

The mobile laboratory consists of two units modular, the first unit? modular houses the virological analysis laboratory consisting of an operator entrance and a product entrance which leads to the actual laboratory, the second unit? ? Utilities technical support.

1.3 DESCRIPTION

The laboratory carries out its activity? in the field of microbiological, virological and immunopathology diagnostics with consultancy services in all the aforementioned disciplines. Biologists or infectious disease specialists who have multidisciplinary expertise can work in it.

The mobile laboratory is characterized as a medical device aimed at prevention and detection in the ?field? for the diagnosis of diseases caused by microorganisms or viruses. It also provides the first analysis in agricultural land of particular problems in infectious diseases by operating as a support to clinical research, diagnostics and counseling.

The mobile laboratory consists of two units modular, the first unit? modular houses the virological analysis laboratory consisting of an operator entrance and a product entrance which leads to the actual laboratory, the second unit? ? the Utilities technical support contained in two standard containers, one the laboratory one, class 45ft 1EEE and the one for services, class 30ft 1BBB, both equipped with hydraulic pistons for the support feet and equipped with three access ramps.

The BLS3-ag class laboratory entirely in class 100 with floor on AISI 316 grids and extraction and injection of clean air from the ceiling and ? designed to prevent any viruses brought inside from spreading to the external environment. Filtration takes place on HEPA filters with an efficiency of 99.995 MPPS in class H14 (standard EN 1822) working in depression at - 2.

Although operators have access to a system of air showers and UV lamps, yes? always looked for a solution to the system of rapid evacuation of personnel from a protected system within 5 seconds. The 1? innovative personnel evacuation system that the laboratory will have? adequate space for two operators provided by a double sealed door with emergency exit function. By opening the first door, the overpressurized air prevents contamination between the two environments and as soon as the first door is closed, the air arrives from a decompressed overpressure with a rapid air washing independent of the laboratory HVAC system with decontamination effect immediate and automatic opening of the second hermetic door with neoprene gasket. The ?passbox emergency? between the two interlocking doors ? REI 120 certificate.

THE BIOTECHNOLOGY LABORATORY.

The whole laboratory will be all conditioned with civil type systems by means of an HVAC system and in pi? the production department will be? Conditioned according to BLS 3.

BASIC PROJECT DATA.

SITE CONDITIONS

For the present study, the following normal environmental conditions of the ground were assumed, to be verified in any case during the executive planning and verification at the displacement sites.

Summer temperature: average 28 ?C

max. 37?C

Winter temperature: min. 1 ?C

average 9 ?C

Humidity? relative: 55% at 32 ?C

Rainfall? annual: 1200 mm

Speed? wind speed: 100 Km/h max

Soil bearing capacity: 0.5 Kg / cm2

Seismic zone: S6

BATTERY LIMITS

At the battery limits of the system, the following must be made available:

* drinking water;

* electric energy;

* methane;

* sewerage.

THE STRUCTURE OF THE LABORATORY AND THE ANNEX PREMISES: DESIGN AND METRIC CALCULATIONS

? a laboratory was designed according to the dictates of clean rooms, to carry out the various operations in an aseptic environment; the internal walls made with Alubond; the perimeter ones made of steel, will be covered in Aisi 316, the internal walls, moreover, will be stiffened with pillars in profile 50 with relative connecting beams of the same type, with the purpose of supporting the pipes of the systems. Will the floor be placed on a Grid in Aisi 316.

OTHER INSTALLATIONS: DESIGN.

Plants serving the laboratory

- General electrical panel and distributions

- Fire-fighting system with smoke detection and relative control unit

- Environmental conditioning system and air exchanges

- Technical gas plant

- Compressed air system

- Sanitary water system and shower and eye wash supply

- General electrical panel and distributions:

The location of this painting will be? in the immediate vicinity of the entrance inside the utility module n? 06.

All the power plants will be sectioned and upstream of each will come? a special differential magneto-thermal type protection is installed, so we will have both a general protection with 0.3 A differential protection and many individual protections with 0.03 A differential protection for each operating area and each sector.

Single lines will be created for emergency lights and escape route indication lights, for the smoke detection system and for any access control system.

All the distribution lines will be protected in special isolated metal walkways, equipped with protective covers, and all the utilities will be derived from them by means of special junction boxes.

The connections to the various utilities will be made with special single-core cables of the flame-retardant type and in compliance with the CEI 20-22 standard; for the branches, where possible, ducts or pipes in self-extinguishing plastic material with the IMQ mark will be used in the appropriate diameters, always leaving at least one third of the ducts free and dividing the control and/or data transmission circuits from the power ones.

Will you provide? to the localized lighting of individual departments or areas with special recessed ceiling lights with IP55 degree of protection, without prejudice to the possibility to be able to isolate an entire area from the general picture.

Particular care will be post for the construction of the general earth network with the installation of pi? equipotential nodes for electrical and exposed metal earths and pipes, suitably connected to special external manholes complete with copper-plated steel spikes and joined together with buried copper braid to create a protection ring for the entire shed.

A separate power line will be? installed to supply the equipment necessary for conditioning the premises and for all technological utilities in general.

The cylinder will be protected by a special cage against atmospheric discharges and the same sar? connected to the general ground loop.

Where necessary, all laboratory utilities with power exceeding 1 KW will be powered by means of interlocked sockets in accordance with CEI standards, therefore the equipment, if not, will be equipped with special plugs in compliance with the law.

Fire-fighting system with smoke detection and relative control unit:

The entire operational area will be protected by smoke detectors, and in particular the entire system will be? divided into the following areas:

1) Area above the airtight false ceiling;

2) Area under the floor;

3) Individual processing rooms (in drawing section 01/2/3/4/5);

4) Utility technological systems area (drawing section 06);

Each of the following sectors will do? head to the alarm management unit of the eight-zone type equipped with a four-call telephone dialer, and a safety device against vandalism and lack of electricity.

The whole system will be connected to special optical and acoustic fire alarm systems by means of external and internal sirens as well as flashing lights.

Will the escape routes be suitably signaled and the position of the emergency lamps will be? such as to facilitate recognition of the aforementioned in the event of a power failure.

All the doors that lead to the outside of the delimited areas or of the various operational zones will be of the watertight type with REI 120 degree of protection and equipped with d? special anti-panic handle.

Because of the particularity of the laboratories in operation and due to the presence in the field of gas with a high combustion and bursting power, any fire-type alarm will trigger? immediately of the electromechanical type solenoid valves to cut off the gas supply upstream of any source of use.

These solenoid valves will be of the manual reset type, as prescribed by the safety regulations, and can only be reset by the personnel in charge after verifying that the danger has actually escaped. Distributed evenly throughout the area, 6 kg powder extinguishers will be installed with a special wall bracket and an indicator sign and, in the immediate vicinity of the access gate, it will be installed a UNI 45 box with a special lance and hose 30 meters long for emergency interventions. During the presentation of the fire practice c/o the competent authorities? of the Fire Brigade will you study? with them the possibility? to install, if necessary, under the floor and/or above the false ceiling, a special automatic extinguishing system of the former Halon gas type in order to prevent the spread of fires in the aforementioned area which is not easily accessible.

- Environmental conditioning system and air exchanges:

Since it is a BLS3 laboratory, the conditioning of these rooms must meet particular conditions such as:

1) Maintaining the right temperature and humidity values? relative

2) Guarantee the correct air exchanges and work in depression with respect to the external environment (-35) 3) Guarantee the non-propagation of substances or gases in the environments

4) Ensure that the releases of excess air into the atmosphere are controlled.

For this purpose, yes? set the following system philosophy:

a) Sar? installed a unit? of Air Treatment (AHU) of the all external air type on ULPA filters in order to guarantee the correct air exchanges.

b) Four-tube fan-coils will be installed to maintain environmental conditions.

c) Sar? an excess air extraction and purification system was installed before it was released into the atmosphere.

Outside air, before being introduced into the rooms, will have the following treatments:

1) Filtration with bag filters and filtration with absolute filters with a yield of 99.999%

2) Transfer to a hot coil powered by a special boiler with a temperature difference of 80-70?C for temperature control in winter.

3) Transfer to a cold coil powered by a special refrigeration unit with a temperature difference of 7-12 ?C for temperature control in the summer.

4) Passage in the humidification area by means of a pump with jet on the lamellar pack to control the humidity? relative.

5) Transfer to the post-heating hot coil both to restore the temperatures in the event of the intervention of the humidifier and to lower the humidity? excess in the summer.

After these treatments the air will come introduced into special ducts with a quadrangular and/or circular section and transported to the individual operating areas; its release into the environment will happen? by special circular anemostats equipped with adjustment shutters for correct calibration.

At the exit from the AHU, instruments will be installed for the continuous control of the temperature and humidity values. These devices, which are part of a control panel, will command the closing and/or opening of the three-way isolating valves of the individual batteries, as well as the intervention of the humidification pump.

The excess air, given by the difference of the incoming air minus that eventually subtracted by the hoods and minus 20% due both to accidental leaks and to the fact that the rooms remain under slight overpressure, will recovery from special vents positioned in the floating floor and ducted.

Sar? sent to an extractor, equipped with a bag filter and an absolute filter with 99.999% efficiency, and will it pass? even on UV lamps before being released into the atmosphere in order to avoid any form of environmental pollution.

Given the installation of absolute filters sar? otherwise? It is also necessary to install differential pressure switches for filter clogging which will signal, on a special luminous panel, the need? replacement of the aforementioned.

The maintenance of the room temperature, which varies due to the dispersions towards the outside and the presence of the personnel, will be? guaranteed in the individual rooms by means of a four-pipe fan-coil and double hot/cold coil: these coils will be fed by means of three-way valves with millimeter adjustment and the same will be managed by the values resulting from the installation of individual room thermostats.

Will the hot and cold feed be? guaranteed by the boiler and the chiller and, therefore, the same will have a potential? such as to meet both the needs of the AHU and of the fan-coil circuit. In fact, wall extractors will be installed in the bathrooms and the entrance doors will be equipped with transit grilles: in this way it will be guaranteed both the exchange of air and the non-stagnation of bad smells.

Only simple radiators will be installed in these rooms to ensure that the temperature is maintained during the winter.

- Supply and installation of UTA for primary air treatment having the following characteristics: a) Filtration department with:

1) bag filters in glass microfibres efficiency 85% ASHRAE 52-76 opacimeter;

2) pre-filter with regenerable cells in pleated synthetic fiber, thickness 48 mm, efficiency 84% particle size;

3) polyhedral absolute filter with small pleats and high filtering medium flow made up of glass fiber paper, efficiency 99.999% DOP.

b) Double intake centrifugal fan with forward blades, with speed? rotation speed 3307 rpm, efficiency 63%, flow rate 1700 mc/h, useful static 50 mm H20.

c) Copper cooling coil with aluminum fins, capacity 1700 mc/h potential? 20.000 Fr/h with fluid 7 - 12 ?C

d) Copper heating coil with aluminum fins, flow rate 1700 m3/h, potential? 26.000 Kcal/h, with fluid 70 - 80 ?C

e) Pack type humidification department with pump made up of alveolar evaporating baffles in pure cellulose, impregnated with thermoplastic resins and additived with preservative agents, efficiency 60-65%.

f) Droplet separator with one fold and two sides in galvanized sheet metal

g) Copper post-heating coil with aluminum fins, flow rate 1700 m3/h, potential? 21.000 Kcal/h, with fluid 70-80 ?C.

h) Galvanized sheet rain cover for outdoor installation;

i) Differential pressure switch for signaling filter clogging of primary brand production;

Technical gas plant:

Will I come made in a special external cylinder - positioned 12 meters away from the structure - for the containment of 4 technological gas storage sectors.

From the cylinder and in a special mobile external cement shaft of the inspectable type, the individual gases will be sent to the utilities through vacuum valves controlled by the alarm management unit located both at the entrance to the shed and before entering under the floating floor.

Will the distribution within the individual rooms of use take place? with pipes in AISI 316; in the immediate vicinity of the positioning of the work benches, the aforementioned will be certified with special closing valves.

Each sector of the cylinder will have? a special rack for the storage of n? 2 cylinders, one of which in operation and one in reserve, complete with first stage reducers to make it possible to transport the various gases to the users.

Compressed air system:

Also needing compressed air, the same will come? produced by a two-stage and two-cylinder electric compressor with a storage tank of 500 It. and the possibility? of continuous pressure up to 8 Ate with a flow rate of about 740 It./min.

Before introducing the compressed air into the pipes, AISI 316, to reach the utilities, the same will come sent to a dryer with freon R134A: all this to avoid the transport of annoying humidity? and the installation of individual binders in the rooms.

Said dryer in accordance with EEC regulations will be? direct expansion type without glycol and complete with separator and automatic condensate drain, maximum operating flow rate 16 bar. Sanitary water system and shower and eye wash supply

In addition to the normal sanitary water system for the toilets made with Aisi 316L coated pipes for the loading and PVC pipes for the discharge, discharge pipes will be prepared for all the sinks of the various laboratories.

A particular line of adduction sar? arranged above the false ceiling to install, in the corridor of the technical laboratories, a shower complete with handle for use by the staff in case it is necessary to immediately wash the clothes for decontamination.

Another supply line will be? prepared under the floor for the installation of n? 2 eye wash units.

1.4 CLAIMS

The innovation of the personnel evacuation module referred to in the previous description is claimed. The 1? innovative personnel evacuation system ?passbox emergency? that the laboratory will have? adequate space for two operators provided by a double sealed door with emergency exit function. By opening the first door, the overpressurized air prevents contamination between the two environments and as soon as the first door is closed, the air arrives from a decompressed overpressure with a rapid air washing independent of the laboratory HVAC system with decontamination effect immediate and automatic opening of the second hermetic door with neoprene gasket.

Currently the emergency exit from the BLS 3-4 laboratories is? constituted or by a collapsible panel or by the normal panic exit door so? as foreseen also by the procedures and guidelines of the WHO, but first of all it is necessary to comply more? to the Australian standards providing for Australian and New Zealand BSL3 and BSL3+ laboratories (AS/NZS 2243.3:2003) which provides guidance on the certification of a level 3 physical containment laboratory the standard states that ?a BSL3 must be constructed in order to achieve, at the time of commissioning, subjecting it to a differential pressure of 200 pascals, an air loss rate of less than 120 L/min. At all times after commissioning, an air leakage rate not exceeding 1200 L/min must be maintained.

The concept of safety in containment laboratories today? intended to be reviewed in the light of the recent Sars and Covid-19 pandemics and the industrial innovation covered by this patent? just to make up for this shortage, why? ? It is true that in the event of an emergency, the personnel must quickly evacuate the area affected by the biological accident, but it is also necessary to prevent the material object of any research from escaping, the release of which would cause contamination of the outside world.

The solution devised? a double door pass box with sanitization inside the box by atomization and with positive controlled pressurization at the entrance of the operator or max 2 operators so that nothing from the laboratory (-2) enters the box (+2) but closed the first door with the operators inside there is an automatic inversion of pressure from positive to negative thanks to the auxiliary system of which ? equipped with the box equal to 4000 m3/min the AHU auxiliary and independent system from the entire laboratory system also acts with ventilation entirely in class 100 ceiling/floor on AISI 316 grids on HEPA H14 filters or on request ULPA U15 filter. Stabilized the negative pressure (-2) in about 1 minute you can? open the second door towards the external environment in total safety.

With the closing of this door, when the personnel exit, the automatic sealing of the entire laboratory module starts with sanitization by atomisation.

Nothing prevents a market solution from being found just for the ?passbox emergency? to be supplied to the various industries of the sector that require its use and a less stringent system can? also be adopted by clean rooms.

1.5. DRAWINGS

The drawings of the invention are attached below:

Claims (1)

The innovation of the personnel evacuation module referred to in the previous description is claimed. The 1? innovative personnel evacuation system ?passbox emergency? that the laboratory will have? adequate space for two operators provided by a double sealed door with emergency exit function. By opening the first door, the overpressurized air prevents contamination between the two environments and as soon as the first door is closed, the air arrives from a decompressed overpressure with a rapid air washing independent of the laboratory HVAC system with decontamination effect immediate and automatic opening of the second hermetic door with neoprene gasket. Currently the emergency exit from the BLS 3-4 laboratories is? constituted or by a collapsible panel or by the normal panic exit door so? as foreseen also by the procedures and guidelines of the WHO, but first of all it is necessary to comply more? to the Australian standards providing for Australian and New Zealand BSL3 and BSL3+ laboratories (AS/NZS 2243.3:2003) which provides guidance on the certification of a level 3 physical containment laboratory the standard states that ?a BSL3 must be constructed in order to achieve, at the time of commissioning, subjecting it to a differential pressure of 200 pascals, an air loss rate of less than 120 L/min. At all times after commissioning, an air leakage rate not exceeding 1200 L/min must be maintained. The concept of safety in containment laboratories today? intended to be reviewed in the light of the recent Sars and Covid-19 pandemics and the industrial innovation covered by this patent? just to make up for this shortage, why? ? It is true that in the event of an emergency, the personnel must quickly evacuate the area affected by the biological accident, but it is also necessary to prevent the material object of any research from escaping, the release of which would cause contamination of the outside world. The solution devised? a double door pass box with sanitization inside the box by atomization and with positive controlled pressurization at the entrance of the operator or max 2 operators so that nothing from the laboratory (-2) enters the box (+2) but closed the first door with the operators inside there is an automatic inversion of pressure from positive to negative thanks to the auxiliary system of which ? equipped with the box equal to 4000 m3/min the AHU auxiliary and independent system from the entire laboratory system also acts with ventilation entirely in class 100 ceiling/floor on AISI 316 grids on HEPA H14 filters or on request ULPA U15 filter. Stabilized the negative pressure (-2) in about 1 minute you can? open the second door towards the external environment in total safety. With the closing of this door, when the personnel exit, the automatic sealing of the entire laboratory module starts with sanitization by atomisation. Nothing prevents a market solution from being found just for the ?passbox emergency? to be supplied to the various industries of the sector that require its use and a less stringent system can? also be adopted by clean rooms.