IT201700077259A1 - Identification of interactions between subcellular organelles by GFP split - Google Patents

Identification of interactions between subcellular organelles by GFP split

Info

Publication number
IT201700077259A1
IT201700077259A1 IT102017000077259A IT201700077259A IT201700077259A1 IT 201700077259 A1 IT201700077259 A1 IT 201700077259A1 IT 102017000077259 A IT102017000077259 A IT 102017000077259A IT 201700077259 A IT201700077259 A IT 201700077259A IT 201700077259 A1 IT201700077259 A1 IT 201700077259A1
Authority
IT
Italy
Prior art keywords
interactions
identification
subcellular organelles
gfp
split
Prior art date
Application number
IT102017000077259A
Other languages
Italian (it)
Inventor
Marisa Brini
Tito Calì
Original Assignee
Univ Degli Studi Padova
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Degli Studi Padova filed Critical Univ Degli Studi Padova
Priority to IT102017000077259A priority Critical patent/IT201700077259A1/en
Priority to PCT/IB2018/055063 priority patent/WO2019012414A1/en
Publication of IT201700077259A1 publication Critical patent/IT201700077259A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/07—Fusion polypeptide containing a localisation/targetting motif containing a mitochondrial localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IT102017000077259A 2017-07-10 2017-07-10 Identification of interactions between subcellular organelles by GFP split IT201700077259A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
IT102017000077259A IT201700077259A1 (en) 2017-07-10 2017-07-10 Identification of interactions between subcellular organelles by GFP split
PCT/IB2018/055063 WO2019012414A1 (en) 2017-07-10 2018-07-10 Identification of interactions between subcellular organelles using split gfp

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102017000077259A IT201700077259A1 (en) 2017-07-10 2017-07-10 Identification of interactions between subcellular organelles by GFP split

Publications (1)

Publication Number Publication Date
IT201700077259A1 true IT201700077259A1 (en) 2019-01-10

Family

ID=60628030

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102017000077259A IT201700077259A1 (en) 2017-07-10 2017-07-10 Identification of interactions between subcellular organelles by GFP split

Country Status (2)

Country Link
IT (1) IT201700077259A1 (en)
WO (1) WO2019012414A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005074436A2 (en) * 2003-10-24 2005-08-18 The Regents Of The University Of California Self-assembling split-fluorescent protein systems
WO2006062877A2 (en) * 2004-12-04 2006-06-15 The Regents Of The University Of California Protein subcellular localization assays using split fluorescent proteins
EP2187213A1 (en) * 2008-11-12 2010-05-19 Korea Basic Science Institute Method for detecting interactions between two and more biological macromolecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005074436A2 (en) * 2003-10-24 2005-08-18 The Regents Of The University Of California Self-assembling split-fluorescent protein systems
WO2006062877A2 (en) * 2004-12-04 2006-06-15 The Regents Of The University Of California Protein subcellular localization assays using split fluorescent proteins
EP2187213A1 (en) * 2008-11-12 2010-05-19 Korea Basic Science Institute Method for detecting interactions between two and more biological macromolecules

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
"METHODS IN ENZYMOLOGY", vol. 543, 1 January 2014, ACADEMIC PRESS, US, ISSN: 0076-6879, article DENIS OTTOLINI ET AL: "Methods to Measure Intracellular Ca2+ Fluxes with Organelle-Targeted Aequorin-Based Probes", pages: 21 - 45, XP055459032, DOI: 10.1016/B978-0-12-801329-8.00002-7 *
CABANTOUS S ET AL: "Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein", NATURE BIOTECHNOLOGY, GALE GROUP INC, vol. 23, no. 1, 5 December 2004 (2004-12-05), pages 102 - 107, XP002392679, ISSN: 1087-0156, DOI: 10.1038/NBT1044 *
CSORDAS GYOERGY ET AL: "Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial Interface", MOLECULAR CELL, vol. 39, no. 1, 9 July 2010 (2010-07-09), pages 121 - 132, XP002779277, ISSN: 1097-2765 *
D. CIERI ET AL: "SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition", CELL DEATH & DIFFERENTIATION, 11 December 2017 (2017-12-11), XP002779279, Retrieved from the Internet <URL:https://www.nature.com/articles/s41418-017-0033-z.pdf> [retrieved on 20180316], DOI: 10.1038/s41418-017-0033-z *
R. RIZZUTO ET AL: "Close Contacts with the Endoplasmic Reticulum as Determinants of Mitochondrial Ca2+ Responses", SCIENCE, vol. 280, no. 5370, 12 June 1998 (1998-06-12), pages 1763 - 1766, XP002779278, DOI: 10.1126/science.280.5370.1763 *
RICCARDO FILADI ET AL: "Mitofusin 2 ablation increases endoplasmic reticulum-mitochondria coupling", PROCEEDINGS NATIONAL ACADEMY OF SCIENCES PNAS, vol. 112, no. 17, 13 April 2015 (2015-04-13), US, pages E2174 - E2181, XP055459215, ISSN: 0027-8424, DOI: 10.1073/pnas.1504880112 *
RIZZUTO R ET AL: "Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells", CURRENT BIO, CURRENT SCIENCE, GB, vol. 5, no. 6, 1 June 1995 (1995-06-01), pages 635 - 642, XP004585182, ISSN: 0960-9822, DOI: 10.1016/S0960-9822(95)00128-X *
RIZZUTO R ET AL: "DOUBLE LABELLING OF SUBCELLULAR STRUCTURES WITH ORGANELLE-TARGETED GFP MUTANTS IN VIVO", CURRENT BIOLOGY, CURRENT SCIENCE, GB, vol. 6, no. 2, 1 February 1996 (1996-02-01), pages 183 - 188, XP000610252, ISSN: 0960-9822, DOI: 10.1016/S0960-9822(02)00451-7 *
XIE W ET AL: "A Split-GFP Gateway CloningSystem for Topology Analyses of MembraneProteins in Plants", PLOS ONE, vol. 12, no. 1, E0170118, 13 January 2017 (2017-01-13), pages 1 - 8, XP002779280, DOI: 10.1371/journal.pone.0170118 *
ZHANG SHIFANG ET AL: "Combinatorial marking of cells and organelles with reconstituted fluorescent proteins", CELL, CELL PRESS, vol. 119, no. 1, 1 October 2004 (2004-10-01), pages 137 - 144, XP002484930, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2004.09.012 *

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Publication number Publication date
WO2019012414A1 (en) 2019-01-17

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