IT1408248B1 - Terreno di coltura e metodo per l estrazione, l isolamento e la propagazione di linee cellulari endoteliali animali ad elevata purezza e linee cellulari animali continue altamente purificate così ottenute - Google Patents

Terreno di coltura e metodo per l estrazione, l isolamento e la propagazione di linee cellulari endoteliali animali ad elevata purezza e linee cellulari animali continue altamente purificate così ottenute

Info

Publication number
IT1408248B1
IT1408248B1 ITMI2011A000201A ITMI20110201A IT1408248B1 IT 1408248 B1 IT1408248 B1 IT 1408248B1 IT MI2011A000201 A ITMI2011A000201 A IT MI2011A000201A IT MI20110201 A ITMI20110201 A IT MI20110201A IT 1408248 B1 IT1408248 B1 IT 1408248B1
Authority
IT
Italy
Prior art keywords
animals
endyelial
propagation
isolation
extraction
Prior art date
Application number
ITMI2011A000201A
Other languages
English (en)
Inventor
Eugenio Agostino Parati
Gloria Invernici
Silvia Cristini
Stefania Elena Navone
Original Assignee
Fond I R C C S Istituto Neurologico Carlo Besta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fond I R C C S Istituto Neurologico Carlo Besta filed Critical Fond I R C C S Istituto Neurologico Carlo Besta
Priority to ITMI2011A000201A priority Critical patent/IT1408248B1/it
Priority to PCT/EP2012/052054 priority patent/WO2012107447A2/en
Priority to EP12702829.8A priority patent/EP2673356A2/en
Publication of ITMI20110201A1 publication Critical patent/ITMI20110201A1/it
Application granted granted Critical
Publication of IT1408248B1 publication Critical patent/IT1408248B1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C12N2501/392—Sexual steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Vascular Medicine (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ITMI2011A000201A 2011-02-11 2011-02-11 Terreno di coltura e metodo per l estrazione, l isolamento e la propagazione di linee cellulari endoteliali animali ad elevata purezza e linee cellulari animali continue altamente purificate così ottenute IT1408248B1 (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ITMI2011A000201A IT1408248B1 (it) 2011-02-11 2011-02-11 Terreno di coltura e metodo per l estrazione, l isolamento e la propagazione di linee cellulari endoteliali animali ad elevata purezza e linee cellulari animali continue altamente purificate così ottenute
PCT/EP2012/052054 WO2012107447A2 (en) 2011-02-11 2012-02-07 Culture medium and method for the extraction, isolation and propagation of animal cell lines of high purity and highly purified continuous animal cell lines thus obtained
EP12702829.8A EP2673356A2 (en) 2011-02-11 2012-02-07 Culture medium and method for the extraction, isolation and propagation of animal cell lines of high purity and highly purified continuous animal cell lines thus obtained

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI2011A000201A IT1408248B1 (it) 2011-02-11 2011-02-11 Terreno di coltura e metodo per l estrazione, l isolamento e la propagazione di linee cellulari endoteliali animali ad elevata purezza e linee cellulari animali continue altamente purificate così ottenute

Publications (2)

Publication Number Publication Date
ITMI20110201A1 ITMI20110201A1 (it) 2012-08-12
IT1408248B1 true IT1408248B1 (it) 2014-06-13

Family

ID=43976107

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI2011A000201A IT1408248B1 (it) 2011-02-11 2011-02-11 Terreno di coltura e metodo per l estrazione, l isolamento e la propagazione di linee cellulari endoteliali animali ad elevata purezza e linee cellulari animali continue altamente purificate così ottenute

Country Status (3)

Country Link
EP (1) EP2673356A2 (it)
IT (1) IT1408248B1 (it)
WO (1) WO2012107447A2 (it)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201507894D0 (en) * 2015-05-08 2015-06-24 Imagen Biotech Ltd Personalised media
CN107043737A (zh) * 2017-06-20 2017-08-15 青岛金典生化器材有限公司 用于培养病毒的无血清培养基及其制备方法

Also Published As

Publication number Publication date
EP2673356A2 (en) 2013-12-18
WO2012107447A3 (en) 2012-11-29
ITMI20110201A1 (it) 2012-08-12
WO2012107447A2 (en) 2012-08-16

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