IT1281879B1 - Method and process for the quantification of biopolymers (polypeptides and polynucleides) on polyacrylamide or agarose plates using staining, decoloration and restaining controlled by silver - Google Patents
Method and process for the quantification of biopolymers (polypeptides and polynucleides) on polyacrylamide or agarose plates using staining, decoloration and restaining controlled by silverInfo
- Publication number
- IT1281879B1 IT1281879B1 IT95PD000092A ITPD950092A IT1281879B1 IT 1281879 B1 IT1281879 B1 IT 1281879B1 IT 95PD000092 A IT95PD000092 A IT 95PD000092A IT PD950092 A ITPD950092 A IT PD950092A IT 1281879 B1 IT1281879 B1 IT 1281879B1
- Authority
- IT
- Italy
- Prior art keywords
- biopolymers
- restaining
- quantification
- polyacrylamide
- staining
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 229920001222 biopolymer Polymers 0.000 title abstract 3
- 229920000936 Agarose Polymers 0.000 title abstract 2
- 229920002401 polyacrylamide Polymers 0.000 title abstract 2
- 238000011002 quantification Methods 0.000 title abstract 2
- 229910052709 silver Inorganic materials 0.000 title abstract 2
- 239000004332 silver Substances 0.000 title abstract 2
- 238000010186 staining Methods 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 241000245026 Scoliopus bigelovii Species 0.000 abstract 1
- 235000012467 brownies Nutrition 0.000 abstract 1
- 238000000326 densiometry Methods 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
This relates to a new method and process for the quantification of biopolymers on polyacrylamide or agarose plates by means of staining, decolouring and restaining controlled by silver. The plates are treated so as to develop a browny black colour in the background which makes it possible to identify any trace components; subsequently the plates are decoloured in order to be able to determine the absolute content of the biopolymers in the various bands by densitometry, starting from those which are least represented.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT95PD000092A IT1281879B1 (en) | 1995-05-10 | 1995-05-10 | Method and process for the quantification of biopolymers (polypeptides and polynucleides) on polyacrylamide or agarose plates using staining, decoloration and restaining controlled by silver |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT95PD000092A IT1281879B1 (en) | 1995-05-10 | 1995-05-10 | Method and process for the quantification of biopolymers (polypeptides and polynucleides) on polyacrylamide or agarose plates using staining, decoloration and restaining controlled by silver |
Publications (3)
Publication Number | Publication Date |
---|---|
ITPD950092A0 ITPD950092A0 (en) | 1995-05-10 |
ITPD950092A1 ITPD950092A1 (en) | 1996-11-10 |
IT1281879B1 true IT1281879B1 (en) | 1998-03-03 |
Family
ID=11391045
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IT95PD000092A IT1281879B1 (en) | 1995-05-10 | 1995-05-10 | Method and process for the quantification of biopolymers (polypeptides and polynucleides) on polyacrylamide or agarose plates using staining, decoloration and restaining controlled by silver |
Country Status (1)
Country | Link |
---|---|
IT (1) | IT1281879B1 (en) |
-
1995
- 1995-05-10 IT IT95PD000092A patent/IT1281879B1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
ITPD950092A0 (en) | 1995-05-10 |
ITPD950092A1 (en) | 1996-11-10 |
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