IT1270197B - Stable mutants of isoamylases - Google Patents
Stable mutants of isoamylasesInfo
- Publication number
- IT1270197B IT1270197B ITMI941209A ITMI941209A IT1270197B IT 1270197 B IT1270197 B IT 1270197B IT MI941209 A ITMI941209 A IT MI941209A IT MI941209 A ITMI941209 A IT MI941209A IT 1270197 B IT1270197 B IT 1270197B
- Authority
- IT
- Italy
- Prior art keywords
- mutants
- isoamylases
- strain
- amino acid
- preparation
- Prior art date
Links
- 108010028688 Isoamylase Proteins 0.000 title abstract 3
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 2
- 125000000539 amino acid group Chemical group 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 239000013600 plasmid vector Substances 0.000 abstract 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 abstract 1
- 229920002472 Starch Polymers 0.000 abstract 1
- 235000015173 baked goods and baking mixes Nutrition 0.000 abstract 1
- 235000013405 beer Nutrition 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 235000020357 syrup Nutrition 0.000 abstract 1
- 239000006188 syrup Substances 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This invention relates to mutants of isoamylases in which at least one of the amino acid residues Thr355, Ile367 and Asp507 is substituted with a different amino acid residue: a plasmid vector containing the nucleotide sequence coding for at least one of the said mutants; a strain of Bacillus transformed by the said plasmid vector; preparation of the said mutants by culturing a strain of the converted Bacillus. The mutant isoamylases are stable at high temperatures (50 degree -53 degree C) at pH 5.0-6.5 and retain their specific activity unchanged. These mutants are useful in the food sector in the production of bakery products, in the manufacture of beer and above all in the preparation of syrups of glucose, maltose and other polysacharides starting from starch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI941209A IT1270197B (en) | 1994-06-09 | 1994-06-09 | Stable mutants of isoamylases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI941209A IT1270197B (en) | 1994-06-09 | 1994-06-09 | Stable mutants of isoamylases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ITMI941209A0 ITMI941209A0 (en) | 1994-06-09 |
| ITMI941209A1 ITMI941209A1 (en) | 1995-12-09 |
| IT1270197B true IT1270197B (en) | 1997-04-29 |
Family
ID=11369089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ITMI941209A IT1270197B (en) | 1994-06-09 | 1994-06-09 | Stable mutants of isoamylases |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1270197B (en) |
-
1994
- 1994-06-09 IT ITMI941209A patent/IT1270197B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ITMI941209A1 (en) | 1995-12-09 |
| ITMI941209A0 (en) | 1994-06-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970613 |