IT1256650B - METHOD FOR THE PREPARATION OF HUMAN LIPOCORTIN 1 - Google Patents
METHOD FOR THE PREPARATION OF HUMAN LIPOCORTIN 1Info
- Publication number
- IT1256650B IT1256650B ITMI922825A ITMI922825A IT1256650B IT 1256650 B IT1256650 B IT 1256650B IT MI922825 A ITMI922825 A IT MI922825A IT MI922825 A ITMI922825 A IT MI922825A IT 1256650 B IT1256650 B IT 1256650B
- Authority
- IT
- Italy
- Prior art keywords
- lipocortin
- preparation
- strain
- culture
- transformed
- Prior art date
Links
- 108090000663 Annexin A1 Proteins 0.000 title abstract 4
- 102000004145 Annexin A1 Human genes 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 101710137500 T7 RNA polymerase Proteins 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Si descrive un processo per la preparazione di Lipocortina 1 ricombinante umana, che comprende:a) la coltura di un ceppo di E. coli recante un gene codificante RNA polimerasi di T7 sotto controllo di un promotore inducibile, detto ceppo essendo stato trasformato da un vettore plasmidiale in cui il gene codificante Lipocortina 1 è sotto controllo di un promotore di T7;b) l'isolamento e la purificazione della Lipocortina 1 ricombinante dalla coltura del ceppo trasformato.A process for the preparation of recombinant human Lipocortin 1 is described, which includes: a) the culture of an E. coli strain carrying a T7 RNA polymerase coding gene under control of an inducible promoter, said strain having been transformed by a vector plasmid in which the coding gene Lipocortin 1 is under control of a T7 promoter; b) the isolation and purification of recombinant Lipocortin 1 from the culture of the transformed strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI922825A IT1256650B (en) | 1992-12-11 | 1992-12-11 | METHOD FOR THE PREPARATION OF HUMAN LIPOCORTIN 1 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI922825A IT1256650B (en) | 1992-12-11 | 1992-12-11 | METHOD FOR THE PREPARATION OF HUMAN LIPOCORTIN 1 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ITMI922825A0 ITMI922825A0 (en) | 1992-12-11 |
| ITMI922825A1 ITMI922825A1 (en) | 1994-06-11 |
| IT1256650B true IT1256650B (en) | 1995-12-12 |
Family
ID=11364447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ITMI922825A IT1256650B (en) | 1992-12-11 | 1992-12-11 | METHOD FOR THE PREPARATION OF HUMAN LIPOCORTIN 1 |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1256650B (en) |
-
1992
- 1992-12-11 IT ITMI922825A patent/IT1256650B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ITMI922825A0 (en) | 1992-12-11 |
| ITMI922825A1 (en) | 1994-06-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19971223 |