IT1250364B - Rapid method for the identification of S. aureus, diagnostic kit for performing such method, monoclonal antibodies against the glucosaminidase of S. aureus and hybridoma for the production of such antibodies - Google Patents
Rapid method for the identification of S. aureus, diagnostic kit for performing such method, monoclonal antibodies against the glucosaminidase of S. aureus and hybridoma for the production of such antibodiesInfo
- Publication number
- IT1250364B IT1250364B ITTO911003A ITTO911003A IT1250364B IT 1250364 B IT1250364 B IT 1250364B IT TO911003 A ITTO911003 A IT TO911003A IT TO911003 A ITTO911003 A IT TO911003A IT 1250364 B IT1250364 B IT 1250364B
- Authority
- IT
- Italy
- Prior art keywords
- aureus
- mab
- hybridoma
- diagnostic kit
- substrate
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000009007 Diagnostic Kit Methods 0.000 title abstract 2
- 210000004408 hybridoma Anatomy 0.000 title abstract 2
- 102000002268 Hexosaminidases Human genes 0.000 title 1
- 108010000540 Hexosaminidases Proteins 0.000 title 1
- 239000000758 substrate Substances 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 238000004458 analytical method Methods 0.000 abstract 3
- 239000000463 material Substances 0.000 abstract 3
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Method for the rapid identification of S. aureus in which the material under analysis together with a substrate is contacted with a MaB directed against the SaG of S. aureus which has been produced by a hybridoma having the characteristics of the DSM... cell line and which has the characteristics of an IgG1; before or after this stage the MaB is coupled with a marker capable of revealing any binding between MaB and the material under analysis, for example through use of the Elisa Technique in which the material under analysis after being contacted with the MaB is contacted with an specific antibody against the MaB used, conjugated with an enzyme and a substrate for the enzyme. The diagnostic kit includes the MaB, a solid substrate (e.g. nitrocellulose) and possibly the anti-MaB antibody conjugated with an enzyme and the substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITTO911003A IT1250364B (en) | 1991-12-20 | 1991-12-20 | Rapid method for the identification of S. aureus, diagnostic kit for performing such method, monoclonal antibodies against the glucosaminidase of S. aureus and hybridoma for the production of such antibodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITTO911003A IT1250364B (en) | 1991-12-20 | 1991-12-20 | Rapid method for the identification of S. aureus, diagnostic kit for performing such method, monoclonal antibodies against the glucosaminidase of S. aureus and hybridoma for the production of such antibodies |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ITTO911003A0 ITTO911003A0 (en) | 1991-12-20 |
| ITTO911003A1 ITTO911003A1 (en) | 1993-06-21 |
| IT1250364B true IT1250364B (en) | 1995-04-07 |
Family
ID=11409808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ITTO911003A IT1250364B (en) | 1991-12-20 | 1991-12-20 | Rapid method for the identification of S. aureus, diagnostic kit for performing such method, monoclonal antibodies against the glucosaminidase of S. aureus and hybridoma for the production of such antibodies |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1250364B (en) |
-
1991
- 1991-12-20 IT ITTO911003A patent/IT1250364B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ITTO911003A1 (en) | 1993-06-21 |
| ITTO911003A0 (en) | 1991-12-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19971218 |