IT1248999B - Labelling of double helix DNA by transamination - Google Patents
Labelling of double helix DNA by transaminationInfo
- Publication number
- IT1248999B IT1248999B IT02076790A IT2076790A IT1248999B IT 1248999 B IT1248999 B IT 1248999B IT 02076790 A IT02076790 A IT 02076790A IT 2076790 A IT2076790 A IT 2076790A IT 1248999 B IT1248999 B IT 1248999B
- Authority
- IT
- Italy
- Prior art keywords
- dna
- double helix
- transamination
- labelling
- helix dna
- Prior art date
Links
- 238000002372 labelling Methods 0.000 title 1
- 238000005891 transamination reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 2
- YTBKXQUWKZTWHJ-UHFFFAOYSA-N 6-(2-aminoethylamino)-1h-pyrimidin-2-one Chemical compound NCCNC=1C=CNC(=O)N=1 YTBKXQUWKZTWHJ-UHFFFAOYSA-N 0.000 abstract 1
- 238000002105 Southern blotting Methods 0.000 abstract 1
- 229960002685 biotin Drugs 0.000 abstract 1
- 239000011616 biotin Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 150000002148 esters Chemical class 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 238000011835 investigation Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Landscapes
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
A method for the preparation of double helix DNA molecules based on the creation of molecules containing N4-aminoethyl-cytosine (4-AEC) is described. After this initial pass the 4-AEC residues can be conjugated with the ester of hydrosuccinimide-biotin, giving rise to biotinylated DNA. The labelled DNA so obtained can be useful as a molecular weight standard, or as a control in Southern Blots. It may also be useful in the investigation of interactions between proteins and DNA for their purification through chromatography procedures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT02076790A IT1248999B (en) | 1990-06-26 | 1990-06-26 | Labelling of double helix DNA by transamination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT02076790A IT1248999B (en) | 1990-06-26 | 1990-06-26 | Labelling of double helix DNA by transamination |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| IT9020767A0 IT9020767A0 (en) | 1990-06-26 |
| IT9020767A1 IT9020767A1 (en) | 1991-12-26 |
| IT1248999B true IT1248999B (en) | 1995-02-11 |
Family
ID=11171774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT02076790A IT1248999B (en) | 1990-06-26 | 1990-06-26 | Labelling of double helix DNA by transamination |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1248999B (en) |
-
1990
- 1990-06-26 IT IT02076790A patent/IT1248999B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| IT9020767A1 (en) | 1991-12-26 |
| IT9020767A0 (en) | 1990-06-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970626 |