IT1248091B - Metodo colorimetrico e soluzione reagente per la determinazione di acido urico in fluidi biologici. - Google Patents
Metodo colorimetrico e soluzione reagente per la determinazione di acido urico in fluidi biologici.Info
- Publication number
- IT1248091B IT1248091B ITMI911695A ITMI911695A IT1248091B IT 1248091 B IT1248091 B IT 1248091B IT MI911695 A ITMI911695 A IT MI911695A IT MI911695 A ITMI911695 A IT MI911695A IT 1248091 B IT1248091 B IT 1248091B
- Authority
- IT
- Italy
- Prior art keywords
- uric acid
- biological fluids
- colorimetric method
- acid
- reactant solution
- Prior art date
Links
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title abstract 3
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title abstract 3
- 239000013060 biological fluid Substances 0.000 title abstract 3
- 229940116269 uric acid Drugs 0.000 title abstract 3
- 238000004737 colorimetric analysis Methods 0.000 title abstract 2
- 239000000376 reactant Substances 0.000 title 1
- 230000001376 precipitating effect Effects 0.000 abstract 2
- 239000006228 supernatant Substances 0.000 abstract 2
- 108010092464 Urate Oxidase Proteins 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 150000003254 radicals Chemical class 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invenzione riguarda un metodo colorimetrico per la determinazione quantitativa dell'acido urico in fluidi biologici che comprende, il precipitare le proteine (deproteinare) contenute in un campione di fluido biologico mediante un precipitante acido, il separare il surnatante deproteinato, il miscelare un'aliquota di detto surnatante con una soluzione reagente stabile contenente il radicale libero colorato ABTS in tampone acido ad un pH da 4,0 a 5,0 ed una densità ottica compresa tra 0,5 e 2,0, ed infine il determinare la diminuzione di colore, correlata alla quantità di acido urico presente nel campione in esame, mediante lettura dell'assorbanza a 410 mm.Il metodo è molto sensibile, specifico, economico, non richiede l'impiego dell'enzima uricasi e può essere condotto manualmente, in semiautomatico o in automatico.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI911695A IT1248091B (it) | 1991-06-20 | 1991-06-20 | Metodo colorimetrico e soluzione reagente per la determinazione di acido urico in fluidi biologici. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI911695A IT1248091B (it) | 1991-06-20 | 1991-06-20 | Metodo colorimetrico e soluzione reagente per la determinazione di acido urico in fluidi biologici. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ITMI911695A0 ITMI911695A0 (it) | 1991-06-20 |
| ITMI911695A1 ITMI911695A1 (it) | 1992-12-20 |
| IT1248091B true IT1248091B (it) | 1995-01-05 |
Family
ID=11360164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ITMI911695A IT1248091B (it) | 1991-06-20 | 1991-06-20 | Metodo colorimetrico e soluzione reagente per la determinazione di acido urico in fluidi biologici. |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1248091B (it) |
-
1991
- 1991-06-20 IT ITMI911695A patent/IT1248091B/it active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ITMI911695A1 (it) | 1992-12-20 |
| ITMI911695A0 (it) | 1991-06-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970530 |