IT1202791B - Polynucleotide e.g. oligo-desoxy-ribonucleotide prepn. - by coupling excess nucleotide having masked 3-phosphate di:ester with nucleoside or nucleotide having free 5-hydroxy gp. - Google Patents
Polynucleotide e.g. oligo-desoxy-ribonucleotide prepn. - by coupling excess nucleotide having masked 3-phosphate di:ester with nucleoside or nucleotide having free 5-hydroxy gp.Info
- Publication number
- IT1202791B IT1202791B IT2951778A IT2951778A IT1202791B IT 1202791 B IT1202791 B IT 1202791B IT 2951778 A IT2951778 A IT 2951778A IT 2951778 A IT2951778 A IT 2951778A IT 1202791 B IT1202791 B IT 1202791B
- Authority
- IT
- Italy
- Prior art keywords
- nucleotide
- prepn
- nucleoside
- masked
- hydroxy
- Prior art date
Links
- 239000002773 nucleotide Substances 0.000 title abstract 5
- 125000003729 nucleotide group Chemical group 0.000 title abstract 5
- 239000002157 polynucleotide Substances 0.000 title abstract 3
- 102000040430 polynucleotide Human genes 0.000 title abstract 3
- 108091033319 polynucleotide Proteins 0.000 title abstract 3
- 230000008878 coupling Effects 0.000 title abstract 2
- 238000010168 coupling process Methods 0.000 title abstract 2
- 238000005859 coupling reaction Methods 0.000 title abstract 2
- 239000002777 nucleoside Substances 0.000 title abstract 2
- 150000003833 nucleoside derivatives Chemical class 0.000 title abstract 2
- 229910019142 PO4 Inorganic materials 0.000 title 1
- 150000002148 esters Chemical class 0.000 title 1
- 239000010452 phosphate Substances 0.000 title 1
- 239000002336 ribonucleotide Substances 0.000 title 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 abstract 2
- 229930024421 Adenine Natural products 0.000 abstract 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- UYTPUPDQBNUYGX-UHFFFAOYSA-N Guanine Natural products O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 abstract 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 1
- 229960000643 adenine Drugs 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000007822 coupling agent Substances 0.000 abstract 1
- IVSXFFJGASXYCL-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=NC=N[C]21 IVSXFFJGASXYCL-UHFFFAOYSA-N 0.000 abstract 1
- 239000005556 hormone Substances 0.000 abstract 1
- 229940088597 hormone Drugs 0.000 abstract 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 230000004936 stimulating effect Effects 0.000 abstract 1
- 229940113082 thymine Drugs 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Prepn. of polynucleotides comprises coupling of (a) a nucleotide having a masked 3'-phosphate diester group with (b) a nucleoside or nucleotide having an available, free 5'-hydroxy gp. in the presence of a coupling agent is characterised in that (a) is used in molar excess (pref. 2:1) w.r.t. (b) so that practically all of (b) is consumed in the reaction. Used in production of alignonucleotides partic. oligodesoxyribonucleotides having a defined sequence of four different nucleotides which makes it possible to insert spent DNA segments in plasmide DNA to code it e.g. for stimulating the production of peptidic (peptide) hormones having specific amino acid e.g. TTT polynucleotide sequence stimulates phenylalanine production, GGA sequence stimulates glycine (where T=thymine, A=adenine and G=guanine).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2951778A IT1202791B (en) | 1978-11-07 | 1978-11-07 | Polynucleotide e.g. oligo-desoxy-ribonucleotide prepn. - by coupling excess nucleotide having masked 3-phosphate di:ester with nucleoside or nucleotide having free 5-hydroxy gp. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2951778A IT1202791B (en) | 1978-11-07 | 1978-11-07 | Polynucleotide e.g. oligo-desoxy-ribonucleotide prepn. - by coupling excess nucleotide having masked 3-phosphate di:ester with nucleoside or nucleotide having free 5-hydroxy gp. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IT7829517A0 IT7829517A0 (en) | 1978-11-07 |
| IT1202791B true IT1202791B (en) | 1989-02-09 |
Family
ID=11227308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT2951778A IT1202791B (en) | 1978-11-07 | 1978-11-07 | Polynucleotide e.g. oligo-desoxy-ribonucleotide prepn. - by coupling excess nucleotide having masked 3-phosphate di:ester with nucleoside or nucleotide having free 5-hydroxy gp. |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1202791B (en) |
-
1978
- 1978-11-07 IT IT2951778A patent/IT1202791B/en active
Also Published As
| Publication number | Publication date |
|---|---|
| IT7829517A0 (en) | 1978-11-07 |
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