IN2015DN03341A - - Google Patents

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Publication number
IN2015DN03341A
IN2015DN03341A IN3341DEN2015A IN2015DN03341A IN 2015DN03341 A IN2015DN03341 A IN 2015DN03341A IN 3341DEN2015 A IN3341DEN2015 A IN 3341DEN2015A IN 2015DN03341 A IN2015DN03341 A IN 2015DN03341A
Authority
IN
India
Prior art keywords
monosaccharide
microorganism
acceptor substrate
reaction
free
Prior art date
Application number
Inventor
Stefan Jennewein
Katja Parschat
Original Assignee
Jennewein Biotechnologie Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jennewein Biotechnologie Gmbh filed Critical Jennewein Biotechnologie Gmbh
Publication of IN2015DN03341A publication Critical patent/IN2015DN03341A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a process for producing a monosaccharide using a microorganism. The microorganism possesses a glycosyltransferase and a glycosidase which work together to synthesise a desired monosaccharide in free form by using an endogenous provided nucleotide activated monosaccharide , glycosylate a suitable acceptor substrate and release the desired monosaccharide by a hydrolysis reaction. The required acceptor substrate for the reaction is recycled and only needed in catalytic amounts. The monosaccharide is produced in free from and is retrieved from the supernatant of the cultivated microorganism.
IN3341DEN2015 2012-10-31 2013-09-09 IN2015DN03341A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12190801.6A EP2728009B1 (en) 2012-10-31 2012-10-31 Process for producing monosaccharides
PCT/EP2013/068579 WO2014067696A1 (en) 2012-10-31 2013-09-09 Process for producing monosacchcarides

Publications (1)

Publication Number Publication Date
IN2015DN03341A true IN2015DN03341A (en) 2015-10-23

Family

ID=47148622

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3341DEN2015 IN2015DN03341A (en) 2012-10-31 2013-09-09

Country Status (9)

Country Link
US (1) US9938549B2 (en)
EP (1) EP2728009B1 (en)
JP (1) JP6165872B2 (en)
KR (1) KR101812018B1 (en)
CN (1) CN104822839B (en)
DK (1) DK2728009T3 (en)
ES (1) ES2640978T3 (en)
IN (1) IN2015DN03341A (en)
WO (1) WO2014067696A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111172220B (en) 2013-09-06 2023-08-04 格礼卡姆股份公司 Fermentation production of oligosaccharides
PL2845905T3 (en) 2013-09-10 2021-09-27 Chr. Hansen HMO GmbH Production of oligosaccharides
US11926858B2 (en) 2014-06-27 2024-03-12 Glycom A/S Oligosaccharide production
CN106460024B (en) 2014-06-27 2021-10-01 格礼卡姆股份公司 Preparation of oligosaccharides
EP3050973A1 (en) 2015-01-30 2016-08-03 Jennewein Biotechnologie GmbH Fermentation process for producing monosaccharides in free form from nucleotide-activated sugars
ES2856749T3 (en) * 2016-10-29 2021-09-28 Chr Hansen Hmo Gmbh Process for the production of fucosylated oligosaccharides
EP3486326A1 (en) 2017-11-21 2019-05-22 Jennewein Biotechnologie GmbH Method for the purification of n-acetylneuraminic acid from a fermentation broth
WO2019222391A1 (en) * 2018-05-15 2019-11-21 The Board Of Trustees Of The University Of Illinois Engineered microorganisms for production of 2' fucosyllactose and l-fucose
EP3569713A1 (en) * 2018-05-16 2019-11-20 Jennewein Biotechnologie GmbH Use of glycosidases in the production of oligosaccharides
EP4179102A1 (en) 2020-07-13 2023-05-17 Glycom A/S Oligosaccharide production
EP4341276A1 (en) 2021-05-17 2024-03-27 DSM IP Assets B.V. Novel technology to enable sucrose utilization in strains for biosyntetic production
CN113699087B (en) * 2021-08-23 2023-08-15 齐鲁工业大学 Lactobacillus plantarum engineering strain for converting lactose to generate lactulose, construction method and application thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000351790A (en) 1999-06-07 2000-12-19 Yaizu Suisankagaku Industry Co Ltd Production of fucose-containing oligosaccharide, or its composition and fucose-containing oligosaccharide or its composition
FR2796082B1 (en) 1999-07-07 2003-06-27 Centre Nat Rech Scient PROCESS FOR PRODUCING OLIGOSACCHARIDES
EP2497478A3 (en) * 2003-12-05 2012-11-07 Children's Hospital Medical Center Oligosaccaride compositions and use thereof in the treatment of infection
KR101054362B1 (en) * 2008-07-03 2011-08-05 재단법인 목암생명공학연구소 How to reduce the fucose content of recombinant protein
JP5726751B2 (en) * 2008-12-19 2015-06-03 イェネヴァイン ビオテヒノロギー ゲーエムベーハー Synthesis of fucosylated compounds
WO2010101158A1 (en) * 2009-03-02 2010-09-10 住友商事株式会社 Novel gene derived from clostridium cellulovorans and use thereof
IT1405680B1 (en) 2010-09-13 2014-01-24 Inalco Spa PROCESS FOR THE PRODUCTION OF L-FUCOSIUM.
EP2479263B1 (en) * 2011-01-20 2013-11-27 Jennewein Biotechnologie GmbH Novel Fucosyltransferases and their applications
CA2827313C (en) * 2011-02-16 2023-08-22 Glycosyn LLC Biosynthesis of human milk oligosaccharides in engineered bacteria

Also Published As

Publication number Publication date
CN104822839A (en) 2015-08-05
EP2728009A1 (en) 2014-05-07
DK2728009T3 (en) 2017-11-06
WO2014067696A1 (en) 2014-05-08
US9938549B2 (en) 2018-04-10
JP2015532832A (en) 2015-11-16
US20150240277A1 (en) 2015-08-27
JP6165872B2 (en) 2017-07-19
ES2640978T3 (en) 2017-11-07
EP2728009B1 (en) 2017-07-26
KR20150097466A (en) 2015-08-26
KR101812018B1 (en) 2018-01-25
CN104822839B (en) 2019-04-12

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