IN2015DN00540A - - Google Patents

Info

Publication number
IN2015DN00540A
IN2015DN00540A IN540DEN2015A IN2015DN00540A IN 2015DN00540 A IN2015DN00540 A IN 2015DN00540A IN 540DEN2015 A IN540DEN2015 A IN 540DEN2015A IN 2015DN00540 A IN2015DN00540 A IN 2015DN00540A
Authority
IN
India
Prior art keywords
microfiltration
protease
ultrafiltration
amylase
amylases
Prior art date
Application number
Inventor
Vincent Courbois
Pierrick Duflot
Aline Lecocq
Jean Marc Verrin
Original Assignee
Roquette Freres
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roquette Freres filed Critical Roquette Freres
Publication of IN2015DN00540A publication Critical patent/IN2015DN00540A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01002—Beta-amylase (3.2.1.2)

Abstract

The present invention concerns an improved method for extracting ß amylases from a soluble fraction of a starch plant said method comprising a step of clarification by microfiltration and a step of concentration and purification by ultrafiltration. This method is characterised in that it uses a protease during the microfiltration step making it possible to greatly reduce the fouling of the membranes used which increases the production time before washing. Simultaneously perfectly clear permeates are obtained which assists with the subsequent ultrafiltration step and makes it possible to improve the transmission of ß amylase. Finally the protease has no negative effect on the ß amylase activity.
IN540DEN2015 2012-08-07 2013-08-06 IN2015DN00540A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1257680A FR2994440B1 (en) 2012-08-07 2012-08-07 PROCESS FOR THE EXTRACTION OF BETA-AMYLASES FROM A SOLUBLE FRACTION OF STARCH PLANT AND IN THE PRESENCE OF A PROTEASE
PCT/FR2013/051899 WO2014023915A1 (en) 2012-08-07 2013-08-06 Method for extracting β-amylases from a soluble fraction of a starch plant and in the presence of a protease.

Publications (1)

Publication Number Publication Date
IN2015DN00540A true IN2015DN00540A (en) 2015-06-26

Family

ID=46963957

Family Applications (1)

Application Number Title Priority Date Filing Date
IN540DEN2015 IN2015DN00540A (en) 2012-08-07 2013-08-06

Country Status (8)

Country Link
US (2) US20150184140A1 (en)
EP (1) EP2882853B1 (en)
CN (1) CN104520427B (en)
ES (1) ES2636895T3 (en)
FR (1) FR2994440B1 (en)
IN (1) IN2015DN00540A (en)
LT (1) LT2882853T (en)
WO (1) WO2014023915A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3001362B1 (en) 2013-01-31 2015-07-10 Roquette Freres PROCESS FOR FRACTIONING PEELE SOLUBLES, FRACTIONS OBTAINED AND THEIR USE
FR3022257B1 (en) * 2014-06-16 2018-03-30 Roquette Freres PROCESS FOR PRODUCING A STABLE AQUEOUS SOLUTION OF BETA-AMYLASE, AQUEOUS SOLUTION OBTAINED AND USES THEREOF
CN110184257B (en) * 2019-07-23 2019-11-12 烟台麦特尔生物技术有限公司 A kind of barley beta-amylase extraction process
CN113481259B (en) * 2021-07-13 2023-06-13 山东省科学院菏泽分院 Method for extracting starch syrup and rice protein isolate by using broken rice
CN116286747B (en) * 2023-05-22 2023-08-08 烟台麦特尔生物技术有限公司 Production process for removing alpha-amylase in beta-amylase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3988348B2 (en) * 2000-03-14 2007-10-10 不二製油株式会社 Method for producing water-soluble polysaccharide and clarification method for water-soluble polysaccharide aqueous solution
FI109358B (en) * 2001-02-06 2002-07-15 Danisco Sugar Oy Method for extracting the enzyme
FR2831168B1 (en) * 2001-10-22 2004-02-06 Rocher Yves Biolog Vegetale PROCESS FOR OBTAINING A NUCLEIC ACID-RICH EXTRACT FROM PLANT MATERIAL
FR2943686B1 (en) * 2009-03-30 2013-11-01 Roquette Freres PROCESS FOR OBTAINING BETA AMYLASE PREPARATION FROM SOLUBLE FRUITS OF STARCH PLANTS

Also Published As

Publication number Publication date
FR2994440B1 (en) 2020-01-31
CN104520427A (en) 2015-04-15
FR2994440A1 (en) 2014-02-14
LT2882853T (en) 2017-10-25
EP2882853B1 (en) 2017-05-31
US20150184140A1 (en) 2015-07-02
US20200165588A1 (en) 2020-05-28
EP2882853A1 (en) 2015-06-17
WO2014023915A1 (en) 2014-02-13
ES2636895T3 (en) 2017-10-10
CN104520427B (en) 2018-05-01

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