IN2014DN09529A - - Google Patents

Download PDF

Info

Publication number
IN2014DN09529A
IN2014DN09529A IN9529DEN2014A IN2014DN09529A IN 2014DN09529 A IN2014DN09529 A IN 2014DN09529A IN 9529DEN2014 A IN9529DEN2014 A IN 9529DEN2014A IN 2014DN09529 A IN2014DN09529 A IN 2014DN09529A
Authority
IN
India
Prior art keywords
coa
dhb
malate
converting
malyl
Prior art date
Application number
Inventor
Thomas Walther
Clémentine Dressaire
Hélène CORDIER
Jean-Marie Francois
Original Assignee
Adisseo France Sas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Adisseo France Sas filed Critical Adisseo France Sas
Publication of IN2014DN09529A publication Critical patent/IN2014DN09529A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01061—4-Hydroxybutyrate dehydrogenase (1.1.1.61)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01075—Malonyl CoA reductase (malonate semialdehyde-forming)(1.2.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01076—Succinate-semialdehyde dehydrogenase (acetylating) (1.2.1.76)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/03—CoA-transferases (2.8.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03024—Malyl-CoA lyase (4.1.3.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01009—Malate--CoA ligase (6.2.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01028—2,3-Dihydro-2,3-dihydroxybenzoate dehydrogenase (1.3.1.28)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention deals with a method for the preparation of 2, 4 - dihydroxybutyric acid (2, 4 -DHB) comprising the successive steps of converting malate , succinyl -CoA and/or glyoxylate into malyl- CoA, converting malyl- CoA, previously obtained into malate- 4- semialdehyde, and converting malate- 4- semialdehyde into 2, 4- DHB using a DHB dehydrogenase.
IN9529DEN2014 2012-04-26 2013-04-25 IN2014DN09529A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IB2012001123 2012-04-26
PCT/IB2013/001071 WO2013160762A2 (en) 2012-04-26 2013-04-25 A method of production of 2,4-dihydroxybutyric acid

Publications (1)

Publication Number Publication Date
IN2014DN09529A true IN2014DN09529A (en) 2015-07-17

Family

ID=48699857

Family Applications (1)

Application Number Title Priority Date Filing Date
IN9529DEN2014 IN2014DN09529A (en) 2012-04-26 2013-04-25

Country Status (10)

Country Link
US (1) US9890400B2 (en)
EP (1) EP2841584B1 (en)
JP (1) JP6342385B2 (en)
KR (1) KR102024109B1 (en)
CN (1) CN104254612B (en)
BR (1) BR112014026149A2 (en)
ES (1) ES2875010T3 (en)
IN (1) IN2014DN09529A (en)
MY (1) MY175391A (en)
WO (1) WO2013160762A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102093172B1 (en) * 2012-07-11 2020-03-25 아디쎄오 프랑스 에스에이에스 Method for the preparation of 2,4-dihydroxybutyrate
CN103642765B (en) * 2013-12-25 2015-11-18 南京工业大学 Alcohol dehydrogenase mutant and application thereof
EP3129489B1 (en) * 2014-04-11 2021-10-13 String Bio Private Limited Production of succinic acid from organic waste or biogas or methane using recombinant methanotrophic bacterium
US10415062B2 (en) 2015-04-07 2019-09-17 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxybutyrate
US10858675B2 (en) 2015-04-07 2020-12-08 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxybutyrate with enhanced 2,4-dihydroxybutyrate efflux
KR102643377B1 (en) * 2015-06-25 2024-03-04 다이나믹 푸드 잉그리다이언츠 코포레이션 Method for producing 2,4-dihydroxybutyric acid
CN105441372A (en) * 2015-10-30 2016-03-30 清华大学 Recombinant corynebacterium glutamicum and application
WO2018053004A2 (en) 2016-09-13 2018-03-22 Allergan, Inc. Non-protein clostridial toxin compositions
CN111778200B (en) * 2019-04-04 2022-11-01 中国科学院微生物研究所 Platform bacterium for producing L-aspartic acid, recombinant bacterium for producing beta-alanine constructed based on platform bacterium and construction method thereof
CN110713962B (en) * 2019-09-06 2022-06-21 南京农业大学 Genetic engineering bacterium for high-yield production of malonyl coenzyme A and construction method and application thereof
DE102021101004B3 (en) 2021-01-19 2022-03-10 Technische Universität Dresden, Körperschaft des öffentlichen Rechts Process for the production of 2,4-dihydroxybutyrate or L-threonine using a microbial pathway
CN115820616A (en) * 2022-07-22 2023-03-21 昆明理工大学 Bacteriophage lyase with fluorescent label and application thereof
CN115948482B (en) * 2023-02-07 2024-02-09 中国科学院天津工业生物技术研究所 Construction method and application of 2, 4-dihydroxybutyric acid biosynthesis pathway

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
EP0260762B1 (en) 1986-09-16 1994-01-12 Gist-Brocades N.V. Penicillium transformants and methods for their production
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
HU218465B (en) 1989-08-09 2000-09-28 Dekalb Genetics Corporation Process and preparation for the production of stabilized transformed fertile monocotyledons and their cells
US5693781A (en) 1991-06-03 1997-12-02 Mitsubishi Chemical Corporation Promoter DNA fragment from coryneform bacteria
AU4712193A (en) 1992-08-19 1994-03-15 Alko Group Limited Fungal promoters active in the presence of glucose
FR2734842B1 (en) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie DNA SEQUENCE OF A HYDROXY-PHENYL PYRUVATE DIOXYGENASE GENE AND OBTAINING PLANTS CONTAINING A HYDROXY-PHENYL PYRUVATE DIOXYGENASE GENE, TOLERANT TO CERTAIN HERBICIDES
FR2736926B1 (en) 1995-07-19 1997-08-22 Rhone Poulenc Agrochimie 5-ENOL PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE MUTEE, CODING GENE FOR THIS PROTEIN AND PROCESSED PLANTS CONTAINING THIS GENE
FR2780416B1 (en) 1998-06-10 2002-12-20 Rhone Poulenc Nutrition Animal INDUSTRIAL PROCESS FOR PRODUCING HETEROLOGOUS PROTEINS IN E. COLI AND STRAINS USEFUL FOR THE PROCESS
FR2787121B1 (en) 1998-12-11 2003-09-12 Aventis Cropscience Sa NOVEL METHOD FOR ISOLATION AND SELECTION OF GENES ENCODING ENZYMES, AND APPROPRIATE CULTURE MEDIUM
AU777674B2 (en) 1999-05-06 2004-10-28 Adisseo France S.A.S. Recombinant Penicillium Funiculosum for homologous and heterologous protein production
JP3687497B2 (en) * 2000-06-28 2005-08-24 ダイソー株式会社 Process for producing optically active 1,2-diols by microbial culture method
DE102006025821A1 (en) * 2006-06-02 2007-12-06 Degussa Gmbh An enzyme for the production of Mehylmalonatsemialdehyd or Malonatsemialdehyd
JP2010501516A (en) 2006-08-24 2010-01-21 エボニック デグサ ゲーエムベーハー Method for producing D, L-2-hydroxy-4-alkylthiobutyric acid
CN1935977B (en) * 2006-10-19 2010-06-09 华东理工大学 Levo lactone hydrolase producing fungus, and its method for preparing chiral hydroxy acid
SG176656A1 (en) 2009-06-04 2012-01-30 Genomatica Inc Microorganisms for the production of 1,4-butanediol and related methods
US9238829B2 (en) * 2010-10-28 2016-01-19 Adisseo France S.A.S. Method of production of 2,4-dihydroxybutyric acid

Also Published As

Publication number Publication date
WO2013160762A3 (en) 2014-01-23
US9890400B2 (en) 2018-02-13
US20150159182A1 (en) 2015-06-11
JP6342385B2 (en) 2018-06-13
KR20150013607A (en) 2015-02-05
WO2013160762A2 (en) 2013-10-31
CN104254612A (en) 2014-12-31
BR112014026149A2 (en) 2017-07-18
KR102024109B1 (en) 2019-09-23
EP2841584A2 (en) 2015-03-04
EP2841584B1 (en) 2021-03-17
MY175391A (en) 2020-06-23
ES2875010T3 (en) 2021-11-08
CN104254612B (en) 2018-06-01
JP2015514433A (en) 2015-05-21

Similar Documents

Publication Publication Date Title
IN2014DN09529A (en)
PL3577110T3 (en) 8-oxetan-3-yl-3,8-diazabicyclo[3.2.1]octan-3-yl substituted compounds as hiv inhibitors
MX2018005829A (en) Compositions for treating the hair.
EP3427822A4 (en) Catalyst for preparing 2,5-furancarboxylic acid and method for preparing 2,5-furancarboxylic acid using catalyst
EP3292116B8 (en) 3-aryl-4-amido-bicyclic [4,5,0]hydroxamic acids as hdac inhibitors
IN2014DN09936A (en)
PL3272860T3 (en) Pyruvate dehydrogenase mutant, microorganism comprising mutant, and method for producing l-amino acid by using microorganism
WO2012059441A3 (en) Method for preparing substituted isoxazoline compounds and their precursors 4-chloro, 4-bromo- or 4-iodobenzaldehyde oximes
EP3181557A4 (en) Carboxylic acid compound, method for preparation thereof, and use thereof
WO2013015663A3 (en) Method for reducing carbon dioxide by using sunlight and hydrogen and apparatus for same
WO2014145897A3 (en) Immunotherapy system and method thereof
EP3336095A4 (en) Oxidized beta-1,4-oligoglucuronic acid, and preparation method therefor and uses thereof
EP3299395A4 (en) Oxidized -1,4-oligoglucuronic acid, and preparation method therefor and uses thereof
EP3643311A4 (en) 4-oxo-alkylated tetramic acid compound, preparation method therefor and use thereof
EP3257939A4 (en) Novel lysine decarboxylase, and method for producing cadaverine by using same
WO2018093223A3 (en) Method for preparing eldecalcitol and intermediate therefor
EP3118312A4 (en) Method for producing 1,5-pentadiamine using increased-thermal-stability lysine decarboxylase mutant
MX2017007259A (en) Metal salt compositions.
EP3106513A4 (en) Method for decomposing polyhydroxyalkanoic acid, and microorganism preparation
MX2016017124A (en) Process for the preparation of 3-hydroxypicolinic acids.
LT2625163T (en) Method for the preparation of (3s,3s`) 4,4`-disulfanediylbis (3-aminobutane 1-sulfonic acid)
MX2019004006A (en) Allulose-containing carbonated water and preparation method therefor.
WO2012119004A3 (en) Parathyroid hormone analogs, compositions and uses thereof
EP3421447A4 (en) Fatty acid compound, preparation method therefor and use thereof
WO2016181204A8 (en) Method of making a mercury based compound, mercury based compound, methods of using the mercury based compound and uses of the mercury based compound