IN2014DE00819AIN819DE2014AIN2014DE00819AIN 2014DE00819 AIN2014DE00819 AIN 2014DE00819AIN 819DE2014 AIN819DE2014 AIN 819DE2014AIN 2014DE00819 AIN2014DE00819 AIN 2014DE00819A
Authority
IN
India
Prior art keywords
domain
sequence encoding
present
gene sequence
scfv
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Application number
Inventor
Venkataramana MUDILI
Balakrishna Konduru
Naveen Kumar KALAGATUR
S Chandranayaka
Harishchandra Sripathy Murali
Harsh Vardhan Batra
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Director General Defence Res And Dev Organization
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Priority to IN819DE2014priorityCriticalpatent/IN2014DE00819A/en
Publication of IN2014DE00819ApublicationCriticalpatent/IN2014DE00819A/en
The present provides for a chimeric gene scFv including a gene sequence encoding for VH domain of antibody produced against Deoxynevolinol, a gene sequence encoding for VL domain of antibody produced against Deoxynevolinol, and a flexible and hydrophobic glycine linker connecting the gene sequence encoding for VH domain and gene sequence encoding for VL domain. The present invention also provides for a process of preparing the chimeric gene scFv and further generating anti DON scFv antibody. The anti-DON-scFv antibody disclosed by the present invention is useful in both detection and protection purposes. The present invention also discloses a dot-ELISA method for quick and cost effective identification of Deoxynevolinol in a sample. Figure 2