IN2014CN04700A - - Google Patents
Info
- Publication number
- IN2014CN04700A IN2014CN04700A IN4700CHN2014A IN2014CN04700A IN 2014CN04700 A IN2014CN04700 A IN 2014CN04700A IN 4700CHN2014 A IN4700CHN2014 A IN 4700CHN2014A IN 2014CN04700 A IN2014CN04700 A IN 2014CN04700A
- Authority
- IN
- India
- Prior art keywords
- chromosome
- mutations
- ptx
- copy
- prn
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K2039/10—Brucella; Bordetella, e.g. Bordetella pertussis; Not used, see subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
BordetellaRecombinant pertussis strains derived from parent strain Tohama are provided. The new strains are obtained by homologous recombination using a allelic exchange vector pSS4245 which allows the replacement of sections of the bacterial chromosome without leaving any accessory mutations. The segment encoding PT subunit SI is replaced to introduce two mutations causing inactivation of the toxic activity of PT. This strain can be further modified to express increased amounts of rPT and/or PRN. A second copy of the ptx cluster of the five PT structural genes of the ptx ptl operon with their promoter and the ptl terminator and containing the above mutations can be inserted elsewhere on the chromosome. In addition a second copy of the PRN gene can be inserted on the chromosome. In both cases abandoned gene loci are selected as the insertion site to avoid the introduction of unwanted genetic alterations.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ZA201109417 | 2011-12-21 | ||
PCT/TH2012/000052 WO2013141823A1 (en) | 2011-12-21 | 2012-12-20 | Modified bordetella pertussis strains |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2014CN04700A true IN2014CN04700A (en) | 2015-09-18 |
Family
ID=49223105
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN4700CHN2014 IN2014CN04700A (en) | 2011-12-21 | 2012-12-20 |
Country Status (9)
Country | Link |
---|---|
US (1) | US9187754B2 (en) |
EP (1) | EP2802646B1 (en) |
JP (1) | JP5966018B2 (en) |
KR (1) | KR101696986B1 (en) |
CN (1) | CN104024400B (en) |
BR (1) | BR112014014950B1 (en) |
ES (1) | ES2642138T3 (en) |
IN (1) | IN2014CN04700A (en) |
WO (1) | WO2013141823A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017176807A2 (en) | 2016-04-04 | 2017-10-12 | University Of Virginia Patent Foundation | Compositions for inhibiting formation of and/or disrupting bacterial biofilms and methods of use therefor |
EP3820457A1 (en) | 2018-07-09 | 2021-05-19 | DBV Technologies | Optimized epicutaneous vaccination |
AU2019376832B2 (en) * | 2018-11-06 | 2024-01-25 | Glaxosmithkline Biologicals Sa | Immunogenic compositions |
WO2024061748A1 (en) * | 2022-09-21 | 2024-03-28 | Prokarium Limited | Salmonella strain with chromosomally integrated landing pad |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5358868A (en) | 1987-11-24 | 1994-10-25 | Connaught Laboratories Limited | Genetic detoxification of pertussis toxin |
US5244657A (en) | 1987-11-24 | 1993-09-14 | Connaught Laboratories Limited | Genetic detoxification of pertussis toxin |
US5221618A (en) | 1987-11-24 | 1993-06-22 | Connaught Laboratories Limited | Genetic detoxification of pertussis toxin |
GB8727489D0 (en) | 1987-11-24 | 1987-12-23 | Connaught Lab | Detoxification of pertussis toxin |
DK0396964T3 (en) * | 1989-04-28 | 1995-10-30 | Sclavo Spa | Pertussis toxin mutants, Bordetella strains capable of producing such mutants, and their use in the development of antipertussis vaccines |
US5786189A (en) * | 1989-11-29 | 1998-07-28 | Smithkline Beecham Biologicals (S.A.) | Vaccine |
GB9115332D0 (en) | 1991-07-16 | 1991-08-28 | Connaught Lab | Manipulation of gene copy number in bordetella |
WO1995009649A1 (en) * | 1993-10-05 | 1995-04-13 | Medeva Holdings B.V. | Vaccine compositions |
US20030072774A1 (en) * | 1994-06-10 | 2003-04-17 | Diane M. Gajewczyk | Proteinaceous adjuvants |
GB9923060D0 (en) * | 1999-09-29 | 1999-12-01 | Chiron Spa | Vaccine |
US20050026866A1 (en) * | 2002-08-02 | 2005-02-03 | Pawelek John M. | Agents and methods for treatment of disease by oligosaccharide targeting agents |
FI116068B (en) * | 2003-09-15 | 2005-09-15 | Fit Biotech Oyj Plc | New selection system, useful vector, bacterial strains and cell selection method |
WO2005090576A1 (en) * | 2004-03-18 | 2005-09-29 | Japan Health Sciences Foundation | Dna construct for preventing pertussis infection |
EP2432884B1 (en) | 2009-05-22 | 2016-08-03 | Merial, Inc. | Antibiotic-free plasmid |
-
2012
- 2012-12-20 BR BR112014014950-0A patent/BR112014014950B1/en active IP Right Grant
- 2012-12-20 KR KR1020147016949A patent/KR101696986B1/en active IP Right Grant
- 2012-12-20 IN IN4700CHN2014 patent/IN2014CN04700A/en unknown
- 2012-12-20 WO PCT/TH2012/000052 patent/WO2013141823A1/en active Application Filing
- 2012-12-20 JP JP2014548741A patent/JP5966018B2/en active Active
- 2012-12-20 CN CN201280064062.8A patent/CN104024400B/en active Active
- 2012-12-20 ES ES12871942.4T patent/ES2642138T3/en active Active
- 2012-12-20 EP EP12871942.4A patent/EP2802646B1/en not_active Revoked
-
2014
- 2014-06-19 US US14/309,807 patent/US9187754B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN104024400A (en) | 2014-09-03 |
US20140302558A1 (en) | 2014-10-09 |
US9187754B2 (en) | 2015-11-17 |
WO2013141823A1 (en) | 2013-09-26 |
EP2802646B1 (en) | 2017-05-10 |
KR20140107290A (en) | 2014-09-04 |
JP2015502758A (en) | 2015-01-29 |
CN104024400B (en) | 2018-02-16 |
ES2642138T3 (en) | 2017-11-15 |
KR101696986B1 (en) | 2017-01-17 |
BR112014014950B1 (en) | 2022-03-29 |
JP5966018B2 (en) | 2016-08-10 |
EP2802646A1 (en) | 2014-11-19 |
BR112014014950A2 (en) | 2020-06-30 |
EP2802646A4 (en) | 2015-08-12 |
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