IN2014CH00475AIN475CH2014AIN2014CH00475AIN 2014CH00475 AIN2014CH00475 AIN 2014CH00475AIN 475CH2014 AIN475CH2014 AIN 475CH2014AIN 2014CH00475 AIN2014CH00475 AIN 2014CH00475A
Authority
IN
India
Prior art keywords
accurate
cervical
detecting
sandwich elisa
cervical cancer
Prior art date
Application number
Inventor
Rajkumar Dr Thangarajan
Mayil Vahanan Bose Mr
Original Assignee
Cancer Inst Wia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cancer Inst WiafiledCriticalCancer Inst Wia
Priority to IN475CH2014priorityCriticalpatent/IN2014CH00475A/en
Publication of IN2014CH00475ApublicationCriticalpatent/IN2014CH00475A/en
TITLE: DETECTION OF CERVICAL PRE-CANCERS USING A NOVEL SANDWICH ELISA APPLICANT NAME: CANCER INSTITUTE ABSTRACT The present invention shall disclose an accurate and reliable method for the early detection of cervical cancer with minimal false result. The method involves collecting the cervical smear samples and detecting the presence of p16 in the collected samples by sandwich ELISA using novel monoclonal antibodies developed against recombinant p16 protein and detecting the presence of cytokeratin using direct ELISA to obtain p160D and cytokeratin OD. From the values of both the ODs the results were ascertained for the accurate presence or absence of cervical cancer. The invention also discloses the method of developing novel monoclonal antibodies against recombinant p16 protein for use in sandwich ELISA and thereby reliably detecting p16 even at 2 picogram level. The invention also discloses a kit for accurate, reliable and early detection of cervical cancer in a subject with minimal false results and avoiding the need for trained Pathologists or CytoPathologists or Cyto-Technologists.