IN2013MU03109AIN3109MU2013AIN2013MU03109AIN 2013MU03109 AIN2013MU03109 AIN 2013MU03109AIN 3109MU2013 AIN3109MU2013 AIN 3109MU2013AIN 2013MU03109 AIN2013MU03109 AIN 2013MU03109A
Authority
IN
India
Prior art keywords
sodium hyaluronate
process includes
clarification
sucrose
precipitation
Prior art date
Application number
Inventor
Kumar Sharma Dr Sanjeev
Conchita Flora Veronica De Souza Ms
Kumar Rakesh
Original Assignee
Unichem Lab Ltd
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Publication date
Application filed by Unichem Lab LtdfiledCriticalUnichem Lab Ltd
Priority to IN3109MU2013priorityCriticalpatent/IN2013MU03109A/en
Publication of IN2013MU03109ApublicationCriticalpatent/IN2013MU03109A/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Polysaccharides And Polysaccharide Derivatives
(AREA)
Abstract
The present invention provides a process for the fermentative production and downstream purification of sodium hyaluronate of pharmaceutical grade and biomedical applications. The process includes bacterial fermentation with carbon sources like starch and sucrose to give Hyaluronic acid of high molecular weight. The process includes purification steps involving cross flow filtration for clarification, treatment with aromatic adsorbent resin and precipitation of sodium hyaluronate with a suitable solvent.