IN2013MU01384AIN1384MU2013AIN2013MU01384AIN 2013MU01384 AIN2013MU01384 AIN 2013MU01384AIN 1384MU2013 AIN1384MU2013 AIN 1384MU2013AIN 2013MU01384 AIN2013MU01384 AIN 2013MU01384A
Authority
IN
India
Prior art keywords
hydantoinase
hydantoin
enzyme
recombinant
selective
Prior art date
Application number
Inventor
Anupama Sureshchandra Engineer
Prashant Kamalakar Dhakephalkar
P Gaikaiwari Raghavendra
Original Assignee
Macs Agharkar Res Inst
Hi Tech Biosciences India Ltd
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Application filed by Macs Agharkar Res Inst, Hi Tech Biosciences India LtdfiledCriticalMacs Agharkar Res Inst
Priority to IN1384MU2013priorityCriticalpatent/IN2013MU01384A/en
Publication of IN2013MU01384ApublicationCriticalpatent/IN2013MU01384A/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Abstract
The present invention provides a process for synthesis of optically pure unnatural carbamoyl amino acids from hydantoin or hydantoin analog or 5-substututed hydantoin derivatives using D-selective hydantoinase enzyme. The process for preparation of recombinant DNA molecule, clone expressing recombinant D-hydantoinase gene, fractionation and purification of recombinant hydantoinase enzyme, biotransformation of hydantoin derivatives are provided in the present invention. Also, methods for increasing the level of expression of hydantoinase enzyme and sequence of D-selective hydantoinase geneand protein are described.