IN2012DN02373A - - Google Patents
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- Publication number
- IN2012DN02373A IN2012DN02373A IN2373DEN2012A IN2012DN02373A IN 2012DN02373 A IN2012DN02373 A IN 2012DN02373A IN 2373DEN2012 A IN2373DEN2012 A IN 2373DEN2012A IN 2012DN02373 A IN2012DN02373 A IN 2012DN02373A
- Authority
- IN
- India
- Prior art keywords
- seq
- dna fragment
- nos
- vector containing
- recombinant vector
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Abstract
The present invention relates to a DNA fragment for improving translation efficiency and a recombinant vector containing the same and more specifically to a DNA fragment which comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-6 SEQ ID NOs: 8-10 SEQ ID NOs: 13 and 14 and SEQ ID NO: 16 and improves the translation efficiency of a heterologous protein placed in the downstream and a recombinant vector containing the DNA fragment. The DNA fragment for improving translation efficiency according to the present invention and a recombinant vector containing the same can improve the translation of a heterologous protein in a transgenic plant.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090081403A KR101161622B1 (en) | 2009-08-31 | 2009-08-31 | DNA fragment to promote translation efficiency and recombinant vectors containing the same |
PCT/KR2010/005669 WO2011025242A2 (en) | 2009-08-31 | 2010-08-25 | Dna fragment for improving translation efficiency, and recombinant vector containing same |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2012DN02373A true IN2012DN02373A (en) | 2015-08-21 |
Family
ID=43628582
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN2373DEN2012 IN2012DN02373A (en) | 2009-08-31 | 2010-08-25 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20120255069A1 (en) |
EP (1) | EP2474614A4 (en) |
KR (1) | KR101161622B1 (en) |
CN (1) | CN102762729A (en) |
IN (1) | IN2012DN02373A (en) |
WO (1) | WO2011025242A2 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101262300B1 (en) * | 2009-10-06 | 2013-05-20 | 헬릭스 주식회사 | Protein vaccine of high pathogenic avian influenza virus derived from transgenic plant and method for preparation thereof |
KR101449155B1 (en) * | 2012-12-06 | 2014-10-13 | 주식회사 바이오앱 | Nucleotide sequence to promote translation efficiency in plants |
CN107075525B (en) * | 2014-05-30 | 2021-06-25 | 纽约市哥伦比亚大学理事会 | Methods for altering expression of polypeptides |
KR101848082B1 (en) * | 2017-05-11 | 2018-04-12 | 주식회사 바이오앱 | Recombinant vector containing cellulose binding domain and Purification method for a protein using thereof |
WO2018222890A1 (en) | 2017-05-31 | 2018-12-06 | Arcturus Therapeutics, Inc. | Synthesis and structure of high potency rna therapeutics |
IT201700105372A1 (en) * | 2017-09-20 | 2019-03-20 | Fondazione St Italiano Tecnologia | FUNCTIONAL NUCLEIC ACID MOLECULE AND ITS USE |
KR102138272B1 (en) | 2018-09-19 | 2020-07-28 | 주식회사 바이오앱 | Recombinant vector comprising BiP fragment and preparation method of recombinant proteins using thereof |
WO2020118115A1 (en) * | 2018-12-06 | 2020-06-11 | Arcturus Therapeutics, Inc. | Compositions and methods for treating ornithine transcarbamylase deficiency |
CN114195866A (en) * | 2020-09-16 | 2022-03-18 | 中国科学院微生物研究所 | Establishment of method for directly reducing protein level in plant |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5290924A (en) * | 1987-02-06 | 1994-03-01 | Last David I | Recombinant promoter for gene expression in monocotyledonous plants |
AU638762B2 (en) * | 1989-10-05 | 1993-07-08 | Optein Inc | Cell-free synthesis and isolation of novel genes and polypeptides |
JP3002724B2 (en) * | 1998-04-10 | 2000-01-24 | 農林水産省蚕糸・昆虫農業技術研究所長 | DNA promoting translation activity into protein and method for efficiently synthesizing protein from protein gene using said DNA |
US6784289B2 (en) * | 1999-12-21 | 2004-08-31 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Translational regulatory elements |
EP1409697B1 (en) * | 2001-07-13 | 2008-04-30 | Sungene GmbH & Co. KGaA | Expression cassettes for the transgenic expression of nucleic acids |
JP4385652B2 (en) * | 2003-05-28 | 2009-12-16 | 株式会社島津製作所 | Translation reaction promoting DNA fragment and template DNA, mRNA and reaction solution for cell-free protein synthesis using the same |
KR100730875B1 (en) * | 2004-12-07 | 2007-06-20 | 가부시키가이샤 시마즈세이사쿠쇼 | DNA fragment to promote translation reaction and method for cell-free system protein synthesis using the same |
-
2009
- 2009-08-31 KR KR1020090081403A patent/KR101161622B1/en not_active IP Right Cessation
-
2010
- 2010-08-25 IN IN2373DEN2012 patent/IN2012DN02373A/en unknown
- 2010-08-25 EP EP10812255A patent/EP2474614A4/en not_active Withdrawn
- 2010-08-25 US US13/393,345 patent/US20120255069A1/en not_active Abandoned
- 2010-08-25 CN CN2010800496543A patent/CN102762729A/en active Pending
- 2010-08-25 WO PCT/KR2010/005669 patent/WO2011025242A2/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
CN102762729A (en) | 2012-10-31 |
KR20110023485A (en) | 2011-03-08 |
KR101161622B1 (en) | 2012-07-04 |
WO2011025242A2 (en) | 2011-03-03 |
EP2474614A2 (en) | 2012-07-11 |
EP2474614A9 (en) | 2013-01-16 |
US20120255069A1 (en) | 2012-10-04 |
EP2474614A4 (en) | 2013-03-13 |
WO2011025242A3 (en) | 2011-08-25 |
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