IN2012DN02373A - - Google Patents

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Publication number
IN2012DN02373A
IN2012DN02373A IN2373DEN2012A IN2012DN02373A IN 2012DN02373 A IN2012DN02373 A IN 2012DN02373A IN 2373DEN2012 A IN2373DEN2012 A IN 2373DEN2012A IN 2012DN02373 A IN2012DN02373 A IN 2012DN02373A
Authority
IN
India
Prior art keywords
seq
dna fragment
nos
vector containing
recombinant vector
Prior art date
Application number
Inventor
Yun Jeong Na
Eun Hyun Jeon
Eun Hye Kwon
Yong Woo Kim
Eun Ju Sohn
In Hwan Hwang
Original Assignee
Helix Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helix Co Ltd filed Critical Helix Co Ltd
Publication of IN2012DN02373A publication Critical patent/IN2012DN02373A/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Abstract

The present invention relates to a DNA fragment for improving translation efficiency  and a recombinant vector containing the same  and more specifically  to a DNA fragment which comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-6  SEQ ID NOs: 8-10  SEQ ID NOs: 13 and 14 and SEQ ID NO: 16  and improves the translation efficiency of a heterologous protein placed in the downstream  and a recombinant vector containing the DNA fragment. The DNA fragment for improving translation efficiency according to the present invention and a recombinant vector containing the same can improve the translation of a heterologous protein in a transgenic plant.
IN2373DEN2012 2009-08-31 2010-08-25 IN2012DN02373A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090081403A KR101161622B1 (en) 2009-08-31 2009-08-31 DNA fragment to promote translation efficiency and recombinant vectors containing the same
PCT/KR2010/005669 WO2011025242A2 (en) 2009-08-31 2010-08-25 Dna fragment for improving translation efficiency, and recombinant vector containing same

Publications (1)

Publication Number Publication Date
IN2012DN02373A true IN2012DN02373A (en) 2015-08-21

Family

ID=43628582

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2373DEN2012 IN2012DN02373A (en) 2009-08-31 2010-08-25

Country Status (6)

Country Link
US (1) US20120255069A1 (en)
EP (1) EP2474614A4 (en)
KR (1) KR101161622B1 (en)
CN (1) CN102762729A (en)
IN (1) IN2012DN02373A (en)
WO (1) WO2011025242A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101262300B1 (en) * 2009-10-06 2013-05-20 헬릭스 주식회사 Protein vaccine of high pathogenic avian influenza virus derived from transgenic plant and method for preparation thereof
KR101449155B1 (en) * 2012-12-06 2014-10-13 주식회사 바이오앱 Nucleotide sequence to promote translation efficiency in plants
CN107075525B (en) * 2014-05-30 2021-06-25 纽约市哥伦比亚大学理事会 Methods for altering expression of polypeptides
KR101848082B1 (en) * 2017-05-11 2018-04-12 주식회사 바이오앱 Recombinant vector containing cellulose binding domain and Purification method for a protein using thereof
WO2018222890A1 (en) 2017-05-31 2018-12-06 Arcturus Therapeutics, Inc. Synthesis and structure of high potency rna therapeutics
IT201700105372A1 (en) * 2017-09-20 2019-03-20 Fondazione St Italiano Tecnologia FUNCTIONAL NUCLEIC ACID MOLECULE AND ITS USE
KR102138272B1 (en) 2018-09-19 2020-07-28 주식회사 바이오앱 Recombinant vector comprising BiP fragment and preparation method of recombinant proteins using thereof
WO2020118115A1 (en) * 2018-12-06 2020-06-11 Arcturus Therapeutics, Inc. Compositions and methods for treating ornithine transcarbamylase deficiency
CN114195866A (en) * 2020-09-16 2022-03-18 中国科学院微生物研究所 Establishment of method for directly reducing protein level in plant

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290924A (en) * 1987-02-06 1994-03-01 Last David I Recombinant promoter for gene expression in monocotyledonous plants
AU638762B2 (en) * 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
JP3002724B2 (en) * 1998-04-10 2000-01-24 農林水産省蚕糸・昆虫農業技術研究所長 DNA promoting translation activity into protein and method for efficiently synthesizing protein from protein gene using said DNA
US6784289B2 (en) * 1999-12-21 2004-08-31 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Translational regulatory elements
EP1409697B1 (en) * 2001-07-13 2008-04-30 Sungene GmbH & Co. KGaA Expression cassettes for the transgenic expression of nucleic acids
JP4385652B2 (en) * 2003-05-28 2009-12-16 株式会社島津製作所 Translation reaction promoting DNA fragment and template DNA, mRNA and reaction solution for cell-free protein synthesis using the same
KR100730875B1 (en) * 2004-12-07 2007-06-20 가부시키가이샤 시마즈세이사쿠쇼 DNA fragment to promote translation reaction and method for cell-free system protein synthesis using the same

Also Published As

Publication number Publication date
CN102762729A (en) 2012-10-31
KR20110023485A (en) 2011-03-08
KR101161622B1 (en) 2012-07-04
WO2011025242A2 (en) 2011-03-03
EP2474614A2 (en) 2012-07-11
EP2474614A9 (en) 2013-01-16
US20120255069A1 (en) 2012-10-04
EP2474614A4 (en) 2013-03-13
WO2011025242A3 (en) 2011-08-25

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