IN2012DE00302A - - Google Patents

Info

Publication number
IN2012DE00302A
IN2012DE00302A IN302DE2012A IN2012DE00302A IN 2012DE00302 A IN2012DE00302 A IN 2012DE00302A IN 302DE2012 A IN302DE2012 A IN 302DE2012A IN 2012DE00302 A IN2012DE00302 A IN 2012DE00302A
Authority
IN
India
Prior art keywords
seq
nos
geranyl pyrophosphate
pyrophosphate synthases
gpps
Prior art date
Application number
Inventor
Shrikant Gupta Vidya
Shridhar Kulkarni Ram
Subhash Pandit Sagar
Prabhakar Giri Ashok
H Pujari Keshav
Original Assignee
Council Scient Ind Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council Scient Ind Res filed Critical Council Scient Ind Res
Priority to PCT/IN2013/000070 priority Critical patent/WO2013114405A1/en
Priority to EP13708533.8A priority patent/EP2809776B1/en
Priority to US14/376,403 priority patent/US20150184136A1/en
Priority to IN302DE2012 priority patent/IN2012DE00302A/en
Publication of IN2012DE00302A publication Critical patent/IN2012DE00302A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01001Dimethylallyltranstransferase (2.5.1.1)

Abstract

The present invention discloses primers for amplifying geranyl pyrophosphate synthases, having sequence selected from the group consisting of Seq. Id. Nos. 2 - 13, from mango. Also disclosed herein is a novel nucleotide sequence of Seq. Id nos. 14 and 15 encoding said amplified geranyl pyrophosphate synthases (GPPS) for enzyme production in an artificial system thus generating the desired flavor in food products.
IN302DE2012 2012-02-03 2013-02-01 IN2012DE00302A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PCT/IN2013/000070 WO2013114405A1 (en) 2012-02-03 2013-02-01 Primer for amplifying geranyl pyrophosphate synthase from mango
EP13708533.8A EP2809776B1 (en) 2012-02-03 2013-02-01 Primer for amplifying geranyl pyrophosphate synthase from mango
US14/376,403 US20150184136A1 (en) 2012-02-03 2013-02-01 Primer for amplifying geranyl pyrophosphate synthase from mango
IN302DE2012 IN2012DE00302A (en) 2012-02-03 2013-02-01

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN302DE2012 IN2012DE00302A (en) 2012-02-03 2013-02-01

Publications (1)

Publication Number Publication Date
IN2012DE00302A true IN2012DE00302A (en) 2015-04-10

Family

ID=47844418

Family Applications (1)

Application Number Title Priority Date Filing Date
IN302DE2012 IN2012DE00302A (en) 2012-02-03 2013-02-01

Country Status (4)

Country Link
US (1) US20150184136A1 (en)
EP (1) EP2809776B1 (en)
IN (1) IN2012DE00302A (en)
WO (1) WO2013114405A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1672061B1 (en) * 1995-09-20 2008-09-10 University of Queensland Nucleotide sequences encoding ACC synthase enzymes of papaya

Also Published As

Publication number Publication date
EP2809776B1 (en) 2016-04-13
EP2809776A1 (en) 2014-12-10
WO2013114405A1 (en) 2013-08-08
US20150184136A1 (en) 2015-07-02

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