IL76827A - Immunoassay for denatured protein analytes specific for linear peptide epitopes such as hemoglobin alc - Google Patents

Immunoassay for denatured protein analytes specific for linear peptide epitopes such as hemoglobin alc

Info

Publication number
IL76827A
IL76827A IL76827A IL7682785A IL76827A IL 76827 A IL76827 A IL 76827A IL 76827 A IL76827 A IL 76827A IL 7682785 A IL7682785 A IL 7682785A IL 76827 A IL76827 A IL 76827A
Authority
IL
Israel
Prior art keywords
linear peptide
immunoassay
binding
peptide epitopes
specific
Prior art date
Application number
IL76827A
Other versions
IL76827A0 (en
Original Assignee
Molecular Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27505324&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL76827(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/763,193 external-priority patent/US4647654A/en
Priority claimed from US06/779,730 external-priority patent/US4658022A/en
Priority claimed from US06/779,731 external-priority patent/US4727036A/en
Application filed by Molecular Diagnostics Inc filed Critical Molecular Diagnostics Inc
Priority to IL9103485A priority Critical patent/IL91034A/en
Publication of IL76827A0 publication Critical patent/IL76827A0/en
Priority to IL91034A priority patent/IL91034A0/en
Publication of IL76827A publication Critical patent/IL76827A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Binding of a particular protein by an antibody reagent involving denaturation of the protein and use of an antibody reagent specific for binding a linear peptide epitope therein. Denaturation by chemical or physical means effectively exposes or enhances the exposure of the linear peptide epitope for binding by the antibody reagent which is preferably raised against a synthetic peptide immunogen. The technique is particularly useful in performing immunoassays for protein, in aqueous test samples. Monoclonal antibodies are also provided which are specific for the glucosylated N-terminal peptide sequence in the beta-subunit of human hemoglobin and are therefore useful in the determination of Hb Alc.
IL76827A 1984-10-29 1985-10-25 Immunoassay for denatured protein analytes specific for linear peptide epitopes such as hemoglobin alc IL76827A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
IL9103485A IL91034A (en) 1984-10-29 1985-10-25 Monoclonal antibodies specific to glucosylated n-terminal peptide sequence in hemoglobin atc
IL91034A IL91034A0 (en) 1984-10-29 1989-07-19 Monoclonal antibodies special for hb alc

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US66581184A 1984-10-29 1984-10-29
US06/763,193 US4647654A (en) 1984-10-29 1985-08-08 Peptides useful in preparing hemoglobin A1c immunogens
US06/779,730 US4658022A (en) 1985-08-08 1985-09-27 Binding of antibody reagents to denatured protein analytes
US06/779,731 US4727036A (en) 1985-08-08 1985-09-27 Antibodies for use in determining hemoglobin A1c

Publications (2)

Publication Number Publication Date
IL76827A0 IL76827A0 (en) 1986-02-28
IL76827A true IL76827A (en) 1990-11-29

Family

ID=27505324

Family Applications (1)

Application Number Title Priority Date Filing Date
IL76827A IL76827A (en) 1984-10-29 1985-10-25 Immunoassay for denatured protein analytes specific for linear peptide epitopes such as hemoglobin alc

Country Status (12)

Country Link
EP (2) EP0185870B2 (en)
JP (2) JPH0723891B2 (en)
AT (2) ATE80951T1 (en)
AU (1) AU594651B2 (en)
CA (1) CA1339952C (en)
DE (2) DE3587687T2 (en)
DK (2) DK167825B1 (en)
ES (2) ES8705633A1 (en)
FI (1) FI84107C (en)
IE (1) IE63731B1 (en)
IL (1) IL76827A (en)
NZ (1) NZ213959A (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK145385D0 (en) * 1985-03-29 1985-04-01 Novo Industri As MONOCLONAL ANTIBODY FOR IMMUNKEMIC ANALYSIS
US5206144A (en) * 1985-03-29 1993-04-27 Novo Industri A/S Determination of glycated (glycosylated) hemoglobin in blood
US4797473A (en) * 1986-06-25 1989-01-10 Regents Of The University Of Minnesota Monoclonal antibodies to unreduced, nonenzymatically-glycated proteins
US5173422A (en) * 1986-08-22 1992-12-22 Miles Inc. Monoclonal antibodies specific for human glycoalbumin
US4822747A (en) * 1986-12-09 1989-04-18 Miles Inc. Polyacrylamide gel particles having hapten moieties bound thereto as immunoassay reagent
JPS63273472A (en) * 1987-04-30 1988-11-10 Asahi Breweries Ltd Novel antibody and detection of human hemoglobin using same
US4970171A (en) * 1987-11-09 1990-11-13 Miles Inc. Denaturant reagents for convenient determination of hemoglobin derivatives in blood
US4847209A (en) * 1987-11-09 1989-07-11 Miles Inc. Latex agglutination immunoassay in the presence of hemoglobin
DE3806198A1 (en) * 1988-02-04 1989-08-17 Boehringer Mannheim Gmbh IMMUNOGENS AND ITS USE TO OBTAIN ANTIBODIES AGAINST HBA (DOWN ARROW) 1 (DOWN ARROW) (DOWN ARROW) C (DOWN ARROW)
IL94724A (en) * 1989-07-13 1994-02-27 Miles Inc Lysis of red blood cells and hemoglobin denaturing by the use of lithium salts
US5484735A (en) * 1989-08-23 1996-01-16 Northwestern University Immunoassay of glycosylated proteins employing antibody directed to reductively glycosylated N-terminal amino acids
DE4206932A1 (en) * 1992-03-05 1993-09-09 Boehringer Mannheim Gmbh IMMUNOLOGICAL METHOD FOR DETERMINING A HAEMOGLOBIN DERIVATIVE
JP2596322B2 (en) * 1992-06-10 1997-04-02 富士レビオ株式会社 Method for measuring the ratio of glycated hemoglobin to total hemoglobin
AU661207B2 (en) * 1992-11-17 1995-07-13 Boehringer Mannheim Gmbh Simultaneous determination of HbA1c and haemoglobin variants with a glycation analogus to HbA1c
EP0711415B1 (en) * 1993-07-28 1998-07-22 Boehringer Mannheim Gmbh Immunoassay for detecting collagen type i or collagen fragments thereof
JP3269765B2 (en) 1995-12-14 2002-04-02 富士写真フイルム株式会社 Method for immunological measurement of hemoglobin derivative and treatment reagent used for the method
EP0975673A2 (en) * 1997-04-21 2000-02-02 Glycozyme, Inc. Determination of recombinant glycosylated proteins and peptides in biological fluids
JPH10332693A (en) * 1997-05-29 1998-12-18 Tokuyama Corp Method for pre-treating specimen
EP1015028A2 (en) 1997-09-19 2000-07-05 Serex, Inc. Methods to improve immunogenicity of antigens and specificity of antibodies
WO1999021015A2 (en) * 1997-10-21 1999-04-29 Cranfield University Affinity ligands, their production and use
JPH11258238A (en) * 1998-03-10 1999-09-24 Tokuyama Corp Reagent kit for measuring immunoagglutination
WO2000008460A1 (en) * 1998-08-07 2000-02-17 Sekisui Chemical Co., Ltd. Method for determining hemoglobins
JP2002005936A (en) * 2000-06-26 2002-01-09 Shino Test Corp Measurement reagent and measurement based on enzyme immunoassay
JP3801133B2 (en) * 2000-07-14 2006-07-26 松下電器産業株式会社 Peptide fructose and its protein conjugate
ATE453668T1 (en) * 2001-04-26 2010-01-15 Dako Denmark As PAN-SPECIFIC MONOCLONAL ANTIBODY DIRECTED AGAINST GLYCOSILATED HEMOGLOBIN
ES2330465T3 (en) 2006-02-03 2009-12-10 Mtm Laboratories Ag PROCEDURE TO PERFORM A DENATURING IMMUNO TEST.
JP5156738B2 (en) * 2006-06-06 2013-03-06 エフ．ホフマン−ラ ロシュ アーゲー Specific hemolysis of whole blood samples
EP2388593A3 (en) * 2006-10-18 2012-01-18 bioMérieux Method for in vitro diagnosis of PVL-producing Staphylococcus aureus
CA2682005C (en) * 2007-06-06 2011-10-25 F. Hoffmann-La Roche Ag Detection of an analyte in a sample of hemolyzed whole blood
US9169320B2 (en) 2008-12-11 2015-10-27 Sekisui Medical Co., Ltd. Antibody to N-terminal region of hemoglobin β-chain
US9341633B2 (en) 2008-12-11 2016-05-17 Sekisui Medical Co., Ltd. Method for pre-treating sample containing glycated hemoglobin
US10670615B2 (en) 2015-12-04 2020-06-02 Polymer Technology Systems, Inc. Systems and methods for interference correction from hemoglobin variants
JP6811581B2 (en) * 2016-10-25 2021-01-13 福井県 Human blood test method and human blood test kit
KR20210068048A (en) 2018-09-28 2021-06-08 세키스이 메디칼 가부시키가이샤 Method for measuring glycated hemoglobin (%)
US20200284807A1 (en) 2019-03-05 2020-09-10 Victor Manneh Saturation binding ratiometric assay

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4247533A (en) * 1978-05-01 1981-01-27 The Rockefeller University Hemoglobin A1c radioimmunoassay
GB1580318A (en) * 1978-05-06 1980-12-03 Univ Rockefeller Antibodies active against human hemoglobin a1c
JPS5610254A (en) * 1979-07-07 1981-02-02 Wako Pure Chem Ind Ltd New rheumatism factor measuring reagent
US4478744A (en) * 1982-01-25 1984-10-23 Sherwood Medical Company Method of obtaining antibodies
JPS58205854A (en) * 1982-05-25 1983-11-30 Mihama Hisaharu Immobilized antibody for occult blood test
US4629692A (en) * 1982-12-06 1986-12-16 Miles Laboratories, Inc. Immunoassay for nonenzymatically glucosylated proteins and protein fragments an index of glycemia
DE3439610A1 (en) * 1984-10-30 1986-04-30 Boehringer Mannheim Gmbh, 6800 Mannheim Immunogens, process for their preparation and antibodies against glycosylated haemoglobin obtained therewith
DK145385D0 (en) * 1985-03-29 1985-04-01 Novo Industri As MONOCLONAL ANTIBODY FOR IMMUNKEMIC ANALYSIS

Also Published As

Publication number Publication date
ES8800435A1 (en) 1987-10-16
IE852655L (en) 1986-04-29
FI854187L (en) 1986-04-30
ES548270A0 (en) 1987-05-01
DE3587687T2 (en) 1994-04-07
DK130791A (en) 1991-07-04
DK165327C (en) 2000-09-11
JP2858534B2 (en) 1999-02-17
DK167825B1 (en) 1993-12-20
DK494085D0 (en) 1985-10-28
EP0185870B1 (en) 1992-09-23
IL76827A0 (en) 1986-02-28
JPH0723891B2 (en) 1995-03-15
EP0185870A3 (en) 1986-08-27
AU4926085A (en) 1986-05-08
ATE98776T1 (en) 1994-01-15
DE3586679D1 (en) 1992-10-29
DE3586679T3 (en) 1998-10-15
EP0185870A2 (en) 1986-07-02
AU594651B2 (en) 1990-03-15
DE3587687D1 (en) 1994-01-27
FI854187A0 (en) 1985-10-25
DK130791D0 (en) 1991-07-04
DK494085A (en) 1986-04-30
EP0316306A3 (en) 1989-10-11
EP0316306A2 (en) 1989-05-17
ES556865A0 (en) 1987-10-16
NZ213959A (en) 1990-03-27
IE63731B1 (en) 1995-06-14
EP0185870B2 (en) 1998-06-17
ES8705633A1 (en) 1987-05-01
JPH0751087A (en) 1995-02-28
ATE80951T1 (en) 1992-10-15
JPS61172064A (en) 1986-08-02
FI84107C (en) 1996-04-11
DK165327B (en) 1992-11-09
EP0316306B1 (en) 1993-12-15
DE3586679T2 (en) 1993-02-18
CA1339952C (en) 1998-07-14
FI84107B (en) 1991-06-28

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