IL42612A - Specific antiserum and method for radioimmunoassay of human chorionic gonadotropin in the presence of luteinizing hormone - Google Patents
Specific antiserum and method for radioimmunoassay of human chorionic gonadotropin in the presence of luteinizing hormoneInfo
- Publication number
- IL42612A IL42612A IL42612A IL4261273A IL42612A IL 42612 A IL42612 A IL 42612A IL 42612 A IL42612 A IL 42612A IL 4261273 A IL4261273 A IL 4261273A IL 42612 A IL42612 A IL 42612A
- Authority
- IL
- Israel
- Prior art keywords
- chorionic gonadotropin
- human chorionic
- serum
- absorbed
- specimen
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Reproductive Health (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Claims (9)
1. A radioimmunoassay anti-serum comprising human chorionic gonadotropin-^ -absorbed anti-human chorionic gonadotropin-serum.
2. The anti-serum oJ? Claim 1 wherein each milliliter of anti-human chorionic gonadotropin-(3 serum was absorbed with 0.1 to 0.4 milligrams of human chorionic gonadotropin-d .
3. A radioimmunological method for quantitatively determining human chorionic gonadotropin in a specimen containing human chorionic gonadotropin in the presence of. up to about 10 times the amount in m I.U./ml. of luteinizing hormone, which comprises providing a human chorionic gonadotropin- (^-absorbed anti-human chorionic gonadotropin-^ serum plus radioimmunolo-gically labeled human chorionic gonadotropin-^ system and determining the degree of inhibition produced by said specimen, on the radioimmunological reaction between said system components, the observed inhibition being substantially due to human chorionic gonadotropin.
4. The method of Claim 3 wherein the inhibition is determined by effecting a radioimmunological assay of the specimen and comparing the results with a known standard.
5. The method of Claim 3 wherein each milliliter of. anti-human chorionic gonadotropin- serum was absorbed with 0.1 to 0.4 milligrams of human chorionic gonadotropin-^.
6. A method for radioimmunologically ascertaining pregnancy which comprises effecting the method of claim 3 using a sample of urine as specimen, pregnancy being positively ascertained when the inhibition is greater than about 20%.
7. A method for radioimmunologically ascertaining pregnancy which comprises effecting the method of claim 5 using a sample of urine as specimen, pregnancy being positively ascertained when the inhibition is greater than about 0%.
8. A method for obtaining an anti-serum capable of radio-immunologically discriminating human chorionic gonadotropin in the presence of up to about 10 times the amount in m I.U./ ml. of luteinizing hormone which comprises obtaining anti-
9. The method, of Claim 8 vherein each milliliter of anti-human chorionic gonadotropin- serum was absorbed v/ith 0.1 to 0.4 milligrams of human chorionic gonadotropin-c .
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT51583/72A IT961450B (en) | 1972-07-17 | 1972-07-17 | SPECIFIC ANTISERUM AND METHOD FOR RADIOIMMUNOLOGICAL DETERMINATION OF HUMAN CHORIONIC GONADOTROPIN IN THE PRESENCE OF LUTEINIZING HORMONE |
Publications (2)
Publication Number | Publication Date |
---|---|
IL42612A0 IL42612A0 (en) | 1973-08-29 |
IL42612A true IL42612A (en) | 1976-12-31 |
Family
ID=11275367
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL42612A IL42612A (en) | 1972-07-17 | 1973-06-26 | Specific antiserum and method for radioimmunoassay of human chorionic gonadotropin in the presence of luteinizing hormone |
Country Status (15)
Country | Link |
---|---|
JP (1) | JPS5312968B2 (en) |
AR (1) | AR196264A1 (en) |
AU (1) | AU459426B2 (en) |
BE (1) | BE802353A (en) |
CA (1) | CA1024069A (en) |
DD (1) | DD107145A5 (en) |
DE (1) | DE2334344C3 (en) |
ES (2) | ES416801A1 (en) |
FR (1) | FR2193491A5 (en) |
GB (1) | GB1369582A (en) |
IL (1) | IL42612A (en) |
IT (1) | IT961450B (en) |
NL (1) | NL7309849A (en) |
SE (1) | SE417372B (en) |
ZA (1) | ZA734544B (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5228928A (en) * | 1975-08-30 | 1977-03-04 | Teikoku Hormone Mfg Co Ltd | Immunochemical reagent and process for its preparation |
US4234561A (en) * | 1978-02-06 | 1980-11-18 | Research Corporation | Antigen for early pregnancy test and contraceptive vaccine |
JPS58151558A (en) * | 1982-03-05 | 1983-09-08 | Takeda Chem Ind Ltd | Method and reagent of immunochemical assay for human villous gonadotropin |
-
1972
- 1972-07-17 IT IT51583/72A patent/IT961450B/en active
-
1973
- 1973-02-11 ES ES416801A patent/ES416801A1/en not_active Expired
- 1973-06-26 IL IL42612A patent/IL42612A/en unknown
- 1973-06-26 CA CA175,000A patent/CA1024069A/en not_active Expired
- 1973-07-04 ZA ZA734544A patent/ZA734544B/en unknown
- 1973-07-06 DE DE2334344A patent/DE2334344C3/en not_active Expired
- 1973-07-11 AU AU58005/73A patent/AU459426B2/en not_active Expired
- 1973-07-12 FR FR7325568A patent/FR2193491A5/fr not_active Expired
- 1973-07-13 GB GB3343073A patent/GB1369582A/en not_active Expired
- 1973-07-13 BE BE133493A patent/BE802353A/en unknown
- 1973-07-16 NL NL7309849A patent/NL7309849A/xx unknown
- 1973-07-16 SE SE7309933A patent/SE417372B/en unknown
- 1973-07-16 AR AR249107A patent/AR196264A1/en active
- 1973-07-16 DD DD172328A patent/DD107145A5/xx unknown
- 1973-07-17 JP JP7998173A patent/JPS5312968B2/ja not_active Expired
-
1975
- 1975-09-15 ES ES440969A patent/ES440969A1/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
IL42612A0 (en) | 1973-08-29 |
DE2334344B2 (en) | 1975-05-22 |
AU5800573A (en) | 1975-02-06 |
DE2334344A1 (en) | 1974-02-07 |
ES416801A1 (en) | 1976-08-01 |
CA1024069A (en) | 1978-01-10 |
JPS4950130A (en) | 1974-05-15 |
ES440969A1 (en) | 1977-03-16 |
AR196264A1 (en) | 1973-12-10 |
ZA734544B (en) | 1974-06-26 |
SE417372B (en) | 1981-03-09 |
DE2334344C3 (en) | 1976-01-08 |
GB1369582A (en) | 1974-10-09 |
FR2193491A5 (en) | 1974-02-15 |
IT961450B (en) | 1973-12-10 |
BE802353A (en) | 1974-01-14 |
AU459426B2 (en) | 1975-03-27 |
NL7309849A (en) | 1974-01-21 |
JPS5312968B2 (en) | 1978-05-06 |
DD107145A5 (en) | 1974-07-12 |
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