IL322610A

IL322610A - Formulation for stabilizing biological materials

Info

Publication number: IL322610A
Application number: IL322610A
Authority: IL
Original assignee: Indigo Ag Inc
Priority date: 2023-02-09
Filing date: 2024-02-09
Publication date: 2025-10-01
Also published as: EP4661678A1; WO2024168313A1; MX2025009278A

Description

WO 2024/168313 PCT/US2024/015273 FORMULATION FOR STABILIZATION OF BIOLOGICAL MATERIALS CROSS REFERENCE TO RELATED APPLICATIONS id="p-1" id="p-1"

[001]This application claims the benefit of U.S. Provisional Application No. 63/484,188, filed February 9, 2023; U.S. Provisional Application No. 63/513,333, filed July 12, 2023; and U.S. Provisional Application No. 63/514,864, filed July 21, 2023. The contents of the aforementioned applications are hereby incorporated by reference in their entirety.

SEQUENCE LISTING [002]The instant application contains a Sequence Listing which has been submitted electronically in XML format and is hereby incorporated by reference in its entirety. Said XML copy, created on February 9, 2024, is named 10261PCTl_Sequence_Listing.xml, and is 187,7bytes in size.

BACKGROUND [003]This invention relates to methods and compositions for improving stability of biological material through manufacturing and distribution of products comprising the same. For example, many beneficial bacteria and fungi are most efficacious as inputs to agricultural crop production when specific concentrations of living organisms are applied. However, many beneficial bacteria and fungi perish quickly within the conditions of manufacturing, distribution, storage, and use.

BRIEF DESCRIPTION OF THE DRAWINGS [004] FIG. 1shows an exemplary method of production and formulation of a spray dried powder intermediate product. The order within in which feedstock components are mixed will not affect survival and/or stability of the microbial concentrate as long as the acidic and alkaline components are mixed and pH checked and adjusted to 6.5-7.2 (at least once) before pH sensitive microorganisms are added. Mixing of the other components may be performed in any order, and the duration of mixing may be adjusted as necessary to ensure complete homogenization. The microbial concentrate may represent any amount of concentration, or no concentration, of microbial biomass (for example, relative to the microbial biomass in growth, production, or extraction media).

WO 2024/168313 PCT/US2024/015273 id="p-5" id="p-5"

[005] FIG. 2shows the titer over time of spray dried powder intermediate product (labeled MUP) comprising MIC-28837 (measured in CFU/g) and water dispersed formulation (labeled WD) MIC-28837 comprising MIC-28837 (measured in CFU/mL), at two temperatures: degrees C and 22 degrees C. The initial starting concentration of MIC-28837 MUP was l.OE+11. The initial starting concentration of MIC-28837 WD was 1.0E+09. Testing of MIC-28837 WD was stopped at approximately 100 days due to contamination overwhelming the sample (a common problem in room temp WDs). [006] FIG. 3Ashows the titer over time of a flowable powder RTU composition comprising spray dried MIC-28837 (labeled F34) applied to corn seeds at an initial dosage of 1.0E+CFU/seed and water dispersed formulation of MIC-28837 (labeled WD) at an initial dosage of 1.0E+05 CFU/seed. The flowable powder RTU composition comprising spray dried MIC-288maintained viability on corn seeds for over 250 days whereas the water dispersed formulation of MIC-28837 lost all detectable viability in fewer than 50 days. [007] FIG. 3Bshows the titer over time of a flowable powder RTU composition comprising spray dried MIC-28837 (labeled F34) applied to soybean seeds at an initial dosage of 1.0E+CFU/seed and water dispersed formulation of MIC-28837 (labeled WD) at an initial dosage of 1.0E+05 CFU/seed. The flowable powder RTU composition comprising spray dried MIC-288maintained viability on soybean seeds for over 250 days whereas the water dispersed formulation of MIC-28837 lost all detectable viability in fewer than 50 days. [008] FIG. 4Ashows the titer over time of two flowable powder RTU compositions (F22 and F27) and a liquid water dispersed composition (Liquid) applied to wheat seeds, each comprising Kosakonia cowanii MIC-70076. MIC-70076 was detected on seeds on day one at 1.79E+CFU/seed (F22) and 1.54E+04 CFU/seed (F27) and 3.88E+04 CFU/seed (Liquid). Flowable powder RTU composition F27 comprising spray dried MIC-70076 maintained viability on wheat seeds for more than 250 days whereas the water dispersed formulation of MIC-70076 lost all detectable viability in fewer than 100 days. This on seed stability assay was conducted in ambient room temperature. [009] FIG. 4Bshows the titer over time of two flowable powder RTU compositions (F22 and F27) and a liquid water dispersed composition (Liquid) applied to wheat seeds, each comprising Kosakonia cowanii MIC-82330. MIC-82330 was detected on seeds on day one at 2.70E+CFU/seed (F22) and 1.75E+04 CFU/seed (F27) and 5.20E+03 CFU/seed (Liquid). Both flowable WO 2024/168313 PCT/US2024/015273 powder RTU composition comprising spray dried MIC-82330 maintained viability on wheat seeds for more than 100 days (more than 500 days for F27) whereas the water dispersed formulation of MIC-82330 lost all detectable viability in fewer than 100 days. This on seed stability assay was conducted in ambient room temperature. [0010] FIG. 5shows the titer over time of flowable powder RTU composition (F27) and a liquid water dispersed composition (Liquid) applied to soy seeds, each comprising Curtobacterium citrenm MIC-99849. MIC-99849 was detected on seeds on day one at 5.74E+03 CFU/seed (F27) and 3.94E+03 CFU/seed (Liquid). The flowable powder RTU composition F27 maintained viability on soy seeds for more than 100 days whereas the water dispersed formulation of MIC- 99849 lost all detectable viability in fewer than 50 days. This on seed stability assay was conducted in ambient room temperature. [0011] FIG. 6shows the titer over time of flowable powder RTU compositions F27, F32, and F34 and a liquid water dispersed composition (Liquid) applied to corn seeds, each comprising Pseudomonas oryzihabitans'NWC-2^31. MIC-28837 was generated under two different fermentation conditions, the data series corresponding to the different fermentation conditions are indicated by circles (condition 1) and triangles (condition 2). MIC-28837 produced with either fermentation condition maintained viability on corn seeds for more than 200 days in all flowable powder RTU compositions whereas the water dispersed formulation of MIC-28837 lost all detectable viability in fewer than 100 days. This on seed stability assay was conducted in ambient room temperature. No additional data points were collected from condition 1 MIC- 28837 on F32 or F27 treated seeds because the experiment was ended at 272 days. At 272 days fermentation condition 1 MIC-28837 was detected on F32 RTU treated seeds at 1.69E+02 and on F27 RTU treated seeds at 1.97E+02. [0012] FIG. 7shows the titer over time of flowable powder RTU compositions F27, F32, and F34 and a liquid water dispersed composition (Liquid) applied to soy seeds, each comprising Pseudomonas oryzdiahitansWiC^^ MIC-28837 was generated under two different fermentation conditions, the data series corresponding to the different fermentation conditions are indicated by circles (condition 1) and triangles (condition 2). MIC-28837 produced with either fermentation condition maintained viability on soy seeds for more than 200 days in all flowable powder RTU compositions whereas the water dispersed formulation of MIC-28837 lost all detectable viability in fewer than 100 days. This on seed stability assay was conducted in WO 2024/168313 PCT/US2024/015273 ambient room temperature. No additional data points were collected from condition 1 MIC- 28837 on F32 or F27 treated seeds because the experiment was ended at 207 days. At 207 days, fermentation condition 1 MIC-28837 was detected on F32 RTU treated seeds at 3.95E+01 and on F27 RTU treated seeds at 2.59E+01.

SUMMARY OF INVENTION [0013]Disclosed herein are synthetic microorganism compositions having improved stability and treatment formulations capable of providing improved stability for a microorganism heterologously disposed therein.[0014] In some embodiments the treatment formulation comprises one or more antioxidant, pH modifier, bulking agent, stabilizer, and solid diluent. An antioxidant is any substance capable of delaying or preventing oxidation of a substrate, for example a vitamin or amino acid. pH modifiers include acidifying, alkalizing and buffering agents. A bulking agent may be any substance which adds volume to the formulation. In some embodiments, the bulking agent is a. polysaccharide. In some embodiments the bulking agent is also a stabilizing agent, for example maltodextrin, sucrose, lactose, trehalose, etc. In some embodiments, the stabilizer is a protein hydrolysate, for example a hydrolyzed vegetable protein from soy. A solid diluent may be any inert solid carrier In some embodiments, the solid diluent is a silica-based material. In some embodiments, a solid diluent has a density of at least 1.0 g/cm A3, at least 1.2 g/cm A3, at least 1.g/cm A3, at least 1.7 g/cn1 A3, at least 2.0 g/cm A3, at least 2.2 g/cm A3, at least 2.5 g/cm A3, at least 2.7 g/cm A3, at least 3.0 g/cm A3, at least 3.2 g/cm A3, or at least 3.5 g/cm A3. In some embodiments, a solid diluent has a density of between 1.0 - 3.0 g/cm A3, between 1.0 - 2.g/cm A3, or between 2.0 - 3.0 g/cm A3. [0015]As used herein, a saccharide is the unit structure of carbohydrates. For example, a saccharide may be a monosaccharide, disaccharide, oligosaccharide, polysaccharide, or combinations thereof. [0016]In some embodiments the formulation comprises multiple components wherein at least one component is selected from each of the following categories: an antioxidant, a pH modifier, a polysaccharide, and a protein hydrolysate. In some embodiments, the formulation additionally comprises a silica-based inert solid. In some embodiments the treatment formulation comprises multiple components wherein at least one component is selected from each of the following WO 2024/168313 PCT/US2024/015273 categories: a vitamin, an amino acid, a pH modifier, a polysaccharide, a protein hydrolysate, and a silica-based inert solid. [0017]In some embodiments the formulation comprises one or more of: at least one antioxidant selected L-ascorbic acid or creatine; at least one amino acid selected from cysteine or glutathione; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose. [0018]In some embodiments, a microorganism is heterologously disposed to a treatment formulation comprising one or more of: at least one antioxidant selected L-ascorbic acid or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of between 3.2-10% by dry weight of the formulation; at least one pH modifier; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose, wherein the at least one saccharide is present in the treatment formulation at a concentration of between 4.2-45% by dry weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of between 4-19.1% by dry weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of between 10-32% by dry weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 3 and 20, for example, between 3-10, over 10, or between 10-20. A treatment formulation optionally comprises one or more of: at least one amino acid selected from cysteine or glutathione, wherein the at least one amino acid is present in the treatment formulation at a concentration of between 0.0-7.0% by dry weight of the formulation; a pH modifier, for example sodium bicarbonate, ascorbic acid, or sodium hydroxide. For example, the at least one pH modifier may be present in the treatment formulation at a concentration of between 1.6-7.9% by dry weight of the formulation. [0019]In some embodiments, the treatment formulation comprises one or more of: at least one antioxidant selected from L-ascorbic acid or creatine, wherein the at least one antioxidant is WO 2024/168313 PCT/US2024/015273 present in the treatment formulation at a concentration of about 6.6% by dry weight; at least one amino acid or peptide selected from cysteine or glutathione, wherein the at least one amino acid is present in the treatment formulation at a concentration of about 3.7% by dry weight; at least one pH modifier selected from sodium bicarbonate, ascorbic acid, or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of about 3.1% by dry weight; at least one saccharide selected from maltodextrin, sucrose, lactose, or trehalose, wherein the at least one saccharide is present in the treatment formulation at a concentration of about 38.9% by dry weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of about 17.9% by dry weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of about 29.9% by dry weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20. [0020]In some embodiments, the treatment formulation comprises one or more of: at least one antioxidant selected from cysteine, glutathione, L-ascorbic acid, or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of between 1.5-3% by wet weight; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of about 0.7% by wet weight; at least one saccharide selected from maltodextrin, sucrose, lactose, or trehalose, wherein the at least one saccharide is present in the treatment formulation at a concentration of about 13% by wet weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of between 1.5-6% by wet weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of about 10% by wet weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20. [0021]In some embodiments, the treatment formulation comprises one or more of: at least one antioxidant selected from cysteine, glutathione, L-ascorbic acid, or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of between 1.5-3% by WO 2024/168313 PCT/US2024/015273 wet weight; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of about 0.7% by wet weight; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose, wherein the at least one saccharide is present in the treatment formulation at a concentration of about 13% by wet weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of between 1.5-6% by wet weight; at least one sugar alcohol such as sorbitol, wherein the at least one sugar alcohol is present in the treatment formulation at a concentration of about 2% by wet weight; at least one non-reducing sugar such as trehalose, wherein the at least one non-reducing sugar is present in the treatment formulation at a concentration of about 2% by wet weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of about 10% by wet weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20. [0022]In some embodiments, the treatment formulation comprises one or more of: at least one antioxidant selected from cysteine, glutathione, L-ascorbic acid, or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of between 1.5-3% by wet weight; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of about 0.7% by wet weight; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose, wherein the at least one saccharide is present in the treatment formulation at a concentration of about 13% by wet weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of between 1.5-6% by wet weight; at least one sugar alcohol such as sorbitol, wherein the at least one sugar alcohol is present in the treatment formulation at a concentration of about 2% by wet weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of about 10% by wet weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20.

WO 2024/168313 PCT/US2024/015273 id="p-23" id="p-23"

[0023]In some embodiments, the treatment formulation comprises one or more of: at least one antioxidant selected from cysteine, glutathione, L-ascorbic acid, or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of between 1.5-3% by wet weight; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of about 0.7% by wet weight; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose, wherein the at least one saccharide is present in the treatment formulation at a concentration of about 13% by wet weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of between 1.5-6% by wet weight; at least one non-reducing sugar such as trehalose, wherein the at least one non-reducing sugar is present in the treatment formulation at a concentration of between 1.2-2.0% by wet weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of about 10% by wet weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 3 and 8. [0024]In some embodiments, the synthetic composition comprises one or more of: a microorganism, wherein the at least one microorganism is present in the treatment formulation at a concentration of between 6.7-7.6% by dry weight; at least one antioxidant selected L-ascorbic acid or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of between 4.4-7.8% by dry weight; at least one amino acid selected from cysteine or glutathione, wherein the at least one amino acid is present in the treatment formulation at a concentration of between 0.0-6.5% by dry weight; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of between 2.1-3.6% by dry weight; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose, wherein the at least one saccharide is present in the treatment formulation at a concentration of between 33.8- 38.4% by dry weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of between 15.6-17.7% by WO 2024/168313 PCT/US2024/015273 dry weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of between 26.0-29.5% by dry weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20. [0025]In some embodiments, the synthetic composition comprises one or more of: a microorganism, wherein the at least one microorganism is present in the treatment formulation at a concentration of about 7.2% by dry weight; at least one antioxidant selected L-ascorbic acid or creatine, wherein the at least one antioxidant is present in the treatment formulation at a concentration of about 6.1% by dry weight; at least one amino acid selected from cysteine or glutathione, wherein the at least one amino acid is present in the treatment formulation at a concentration of about 3.4% by dry weight; at least one pH modifier selected from sodium bicarbonate or sodium hydroxide, wherein the at least one pH modifier is present in the treatment formulation at a concentration of about 2.9% by dry weight; at least one saccharide selected from maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose, wherein the at least one saccharide is present in the treatment formulation at a concentration of about 36.1% by dry weight; at least one protein hydrolysate selected from peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein, for example, from soy, wherein the at least one protein hydrolysate is present in the treatment formulation at a concentration of about 16.7% by dry weight; and at least one inert solid selected from kaolin clay, magnesium stearate, or microcrystalline cellulose, wherein the at least one inert solid is present in the treatment formulation at a concentration of about 27.8% by dry weight. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20. [0026]In some embodiments, the synthetic composition comprising an endophyte as an active ingredient, and at least one antioxidant, and at least one saccharide, wherein the at least one antioxidant is present in the synthetic composition in a ratio of at least 0.07 part antioxidant for each 1 part microbial biomass by dry weight, and the at least one saccharide is present in the synthetic composition in a ratio of at least 2 parts saccharide by for each 1 part microbial biomass dry weight. For example, the at least one antioxidant is a vitamin and/or amino acid. Exemplary antioxidants include ascorbic acid (e.g. L-ascorbic acid), creatine, cysteine, glutathione, or combinations thereof. In some embodiments, at least one antioxidant comprises at least L-ascorbic acid or creatine and at least cysteine or glutathione. In some embodiments, the at WO 2024/168313 PCT/US2024/015273 least one antioxidant is present in the treatment formulation at a ratio of at least 0.1 part antioxidant for each 1 part microbial biomass by dry weight, at a ratio of at least 0.2 part antioxidant for each 1 part microbial biomass by dry weight, at least 0.3 part antioxidant for each part microbial biomass by dry weight, at least 0.4 part antioxidant for each 1 part microbial biomass by dry weight, at least 0.5 part antioxidant for each 1 part microbial biomass by dry weight, at least 0.6 part antioxidant for each 1 part microbial biomass by dry weight, at least 0.part antioxidant for each 1 part microbial biomass by dry weight, at least 0.8 part antioxidant for each 1 part microbial biomass by dry weight, at least 0.9 part antioxidant for each 1 part microbial biomass by dry weight, at least 1 part antioxidant for each 1 part microbial biomass by dry weight, at least 1.2 part antioxidant for each 1 part microbial biomass by dry weight, at least 1.5 part antioxidant for each 1 part microbial biomass by dry weight, at least 2 parts antioxidant for each 1 part microbial biomass by dry weight, or at least 2.5 part antioxidant for each 1 part microbial biomass by dry weight. [0027]In some embodiments, the at least one antioxidant is present in the treatment formulation at a ratio of 0.1-3.0 part antioxidant to 1 part microbial biomass by dry weight, 0.4-2.8 part antioxidant to 1 part microbial biomass by dry weight, 0.4-0.7 part antioxidant to 1 part microbial biomass by dry weight, or 1.5-2.8 part antioxidant to 1 part microbial biomass by dry weight. In some embodiments, ascorbic acid is present in the synthetic composition at a ratio of 0.7-1.4 part ascorbic acid to 1 part microbial biomass by dry weight, cysteine is present in the synthetic composition at a ratio of 0.4-0.7 part cysteine to 1 part microbial biomass by dry weight, and glutathione is present in the synthetic composition at a ratio of 0.4-0.7 part glutathione to 1 part microbial biomass by dry weight. [0028]In some embodiments, at least one saccharide is present in the synthetic composition in a ratio of 0.07-12.4 parts saccharide to 1 part microbial biomass by dry weight. [0029]Exemplary saccharides include maltodextrin, sucrose, lactose, trehalose, microcrystalline cellulose, or combinations thereof. In some embodiments, maltodextrin has a dextrose equivalent (DE) value between 4 and 20. [0030]In some embodiments, the at least one saccharide is present in the treatment formulation at a ratio of at least 0.1 parts saccharide for each 1 part microbial biomass by dry weight, at least 0.2 parts saccharide for each 1 part microbial biomass by dry weight, at least 0.3 parts saccharide for each 1 part microbial biomass by dry weight, at least 0.4 parts saccharide for each 1 part WO 2024/168313 PCT/US2024/015273 microbial biomass by dry weight, at least 0.5 parts saccharide for each 1 part microbial biomass by dry weight, at least 0.75 parts saccharide for each 1 part microbial biomass by dry weight, at least 1 parts saccharide for each 1 part microbial biomass by dry weight, at least 1.2 parts saccharide for each 1 part microbial biomass by dry weight, at least 1.4 parts saccharide for each part microbial biomass by dry weight, at least 1.6 parts saccharide for each 1 part microbial biomass by dry weight, at least 2 parts saccharide for each 1 part microbial biomass by dry weight, at least 2.5 parts saccharide for each 1 part microbial biomass by dry weight, at least parts saccharide for each 1 part microbial biomass by dry weight, at least 4 parts saccharide for each 1 part microbial biomass by dry weight, at least 5 parts saccharide for each 1 part microbial biomass by dry weight, at least 6 parts saccharide for each 1 part microbial biomass by dry weight, at least 7 parts saccharide for each 1 part microbial biomass by dry weight, at least parts saccharide for each 1 part microbial biomass by dry weight, at least 9 parts saccharide for each 1 part microbial biomass by dry weight, at least 10 parts saccharide for each 1 part microbial biomass by dry weight, at least 11 parts saccharide for each 1 part microbial biomass by dry weight, or at least 12 parts saccharide for each 1 part microbial biomass by dry weight. [0031]In some embodiments, the at least one saccharide is present in the treatment formulation at a ratio of between 0.35-2.8 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 0.62-12.4 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 2-5 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 3-5 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 3-7 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 3-10 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio ofbetween 4-12 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio ofbetween 5-7 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 6-10 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio ofbetween 8-12 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio ofbetween 6-14 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio ofbetween 8-14 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio ofbetween 12-30 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 20-50 parts saccharide for each 1 part microbial biomass by dry weight, at a ratio of between 40-62 parts saccharide for each 1 part microbial biomass by dry weight, or at a ratio of WO 2024/168313 PCT/US2024/015273 between 20-62 parts saccharide for each 1 part microbial biomass by dry weight. In some embodiments, maltodextrin is present in the synthetic composition at a ratio of 3.1-9.3 parts maltodextrin to 1 part microbial biomass by dry weight. In some embodiments, maltodextrin is present in the synthetic composition at a ratio of 0.62-12.4 parts maltodextrin to 1 part microbial biomass by dry weight. In some embodiments, maltodextrin is present in the synthetic composition at a ratio of 12-40 parts maltodextrin to 1 part microbial biomass by dry weight. In some embodiments, maltodextrin is present in the synthetic composition at a ratio of 30-64 parts maltodextrin to 1 part microbial biomass by dry weight. [0032]In some embodiments, a synthetic composition comprises at least one protein hydrolysate, wherein the at least one protein hydrolysate is present in the synthetic composition in a ratio of at least 0.07 part protein hydrolysate for each 1 part microbial biomass by dry weight, at least 0.2 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 0.5 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 1.2 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 1.4 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 2 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 3 parts protein hydrolysate for each part microbial biomass by dry weight, at least 4 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 5 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 7 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 10 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 13 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 18 parts protein hydrolysate for each 1 part microbial biomass by dry weight, at least 25 parts protein hydrolysate for each 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 0.24-5.8 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 1.2-4.35 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 1.45-4.35 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one WO 2024/168313 PCT/US2024/015273 protein hydrolysate is present in the synthetic composition in a ratio of 1.2-3.6 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 0.35-1.05 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 4-10 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 8-12 parts protein hydrolysate to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is present in the synthetic composition in a ratio of 12-30 parts protein hydrolysate to 1 part microbial biomass by dry weight. For example, at least one protein hydrolysate may be one or more amino acids, a peptone, tryptone, casamino acids, hydrolyzed vegetable protein, or combinations thereof. In some embodiments, at least one protein hydrolysate is a peptone, and the peptone is present in the synthetic composition at a ratio of 0.24-5.8 parts peptone to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 0.07-7 parts casamino acids to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 0.07-1.4 parts casamino acids to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 0.07-1.4 parts casamino acids to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 1-5 parts casamino acids to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 2-7 parts casamino acids to part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is a peptone, and the peptone is present in the synthetic composition at a ratio of 5-8 parts peptone to part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is a peptone, and the peptone is present in the synthetic composition at a ratio of 7-10 parts peptone to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is a peptone, and the peptone is present in the synthetic composition at a ratio of 9- WO 2024/168313 PCT/US2024/015273 parts peptone to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is a peptone, and the peptone is present in the synthetic composition at a ratio of 14-30 parts peptone to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 0.07-1.4 parts casamino acids to 1 part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 1-5 parts casamino acids to part microbial biomass by dry weight. In some embodiments, at least one protein hydrolysate is casamino acids, and the casamino acids are present in the synthetic composition at a ratio of 2-parts casamino acids to 1 part microbial biomass by dry weight. [0033]In some embodiments, a synthetic composition comprises at least an inert solid. For example, an inert solid may be kaolin clay, magnesium stearate, microcrystalline cellulose, or combinations thereof. In some embodiments, least one inert solid is present in the synthetic composition in a ratio of at least 0.4 parts inert solid to 1 part microbial biomass by dry weight, at least 1 parts inert solid to 1 part microbial biomass by dry weight, at least 2 parts inert solid to part microbial biomass by dry weight, at least 3 parts inert solid to 1 part microbial biomass by dry weight, at least 4 parts inert solid to 1 part microbial biomass by dry weight, at least 4 parts inert solid to 1 part microbial biomass by dry weight, at least 5 parts inert solid to 1 part microbial biomass by dry weight, at least 6 parts inert solid to 1 part microbial biomass by dry weight, at least 7 parts inert solid to 1 part microbial biomass by dry weight, at least 8 parts inert solid to 1 part microbial biomass by dry weight, at least 9 parts inert solid to 1 part microbial biomass by dry weight, at least 10 parts inert solid to 1 part microbial biomass by dry weight, at least 20 parts inert solid to 1 part microbial biomass by dry weight, at least 50 parts inert solid to part microbial biomass by dry weight, at least 100 parts inert solid to 1 part microbial biomass by dry weight. In some embodiments, at least one inert solid is present in the synthetic composition in a ratio of between 2-8 parts inert solid to 1 part microbial biomass by dry weight. In some embodiments, at least one inert solid is present in the synthetic composition in a ratio of between 2-8 parts inert solid to 1 part microbial biomass by dry weight. In some embodiments, at least one inert solid is present in the synthetic composition in a ratio of between 6-12 parts inert solid to 1 part microbial biomass by dry weight. In some embodiments, at least one inert solid is present in the synthetic composition in a ratio of between 10-20 parts inert solid to 1 part WO 2024/168313 PCT/US2024/015273 microbial biomass by dry weight. In some embodiments, at least one inert solid is present in the synthetic composition in a ratio of between 18-30 parts inert solid to 1 part microbial biomass by dry weight. In some embodiments, at least one inert solid is present in the synthetic composition in a ratio of between 20-50 parts inert solid to 1 part microbial biomass by dry weight. [0034]In some embodiments, a synthetic composition comprises at least one pH modifier, for example, sodium bicarbonate or sodium hydroxide. In some embodiments, a synthetic composition comprises sodium bicarbonate, and the sodium bicarbonate is present in the synthetic composition at a ratio of 0.1-1.05 parts sodium bicarbonate to 1 part microbial biomass by dry weight. In some embodiments, a synthetic composition comprises sodium bicarbonate, and the sodium bicarbonate is present in the synthetic composition at a ratio of 0.03-1.45 parts sodium bicarbonate to 1 part microbial biomass by dry weight. In some embodiments, a synthetic composition comprises sodium bicarbonate, and the sodium bicarbonate is present in the synthetic composition at a ratio of 0.03-7 parts sodium bicarbonate to 1 part microbial biomass by dry weight. In some embodiments, a synthetic composition comprises sodium bicarbonate, and the sodium bicarbonate is present in the synthetic composition at a ratio of 1-4 parts sodium bicarbonate to 1 part microbial biomass by dry weight. In some embodiments, a synthetic composition comprises sodium bicarbonate, and the sodium bicarbonate is present in the synthetic composition at a ratio of 3-8 parts sodium bicarbonate to 1 part microbial biomass by dry weight. [0035]In some embodiments, a synthetic composition further comprises at least one of talc and mineral oil. [0036]In some embodiments, maltodextrin has a low dextrose equivalent (DE) value, for example about 3. In some embodiments, maltodextrin has a moderate DE value, for example about 10. In some embodiments, maltodextrin has a high DE value, for example about 18. In some embodiments, maltodextrin has a DE value between 4 and 10. In some embodiments maltodextrin has DE value between 10 and 20. In some embodiments maltodextrin has DE value of at least 4, a DE value of at least 6, a DE value of at least 8, a DE value of at least 10, a DE value of at least 14, or a DE value of at least 18. In some embodiments maltodextrin has a DE value of between about 4 and 20, a DE value of between about 3 and 6, a DE value of between about 6 and 14, and a DE value of between about 14 and 20. In some embodiments, a DE value is an average DE value.

WO 2024/168313 PCT/US2024/015273 id="p-37" id="p-37"

[0037]In some embodiments, the antioxidant is water soluble. In some embodiments, the antioxidant is L-ascorbic acid, creatine, citric acid, uric acid, glutathione, etc. [0038]In some embodiments, the protein hydrolysates are vegetable based (for example, soybean, etc.), animal protein based (e.g. eggs, casein, etc.), fungal based (for example, yeast extract, hyphal biomass, etc.) [0039]In some embodiments, the formulation additionally comprises a yeast extract and/or whey powder. [0040]In some embodiments, the saccharide component is maltodextrin. In some embodiments, the maltodextrin is derived from a plant such as rice, corn, wheat, potato, cassava (for example, tapioca), etc. In some embodiments, the saccharide component is a polymeric saccharide. In some embodiments the polymeric saccharide is branching, non-branching, etc. [0041]In some embodiments, a synthetic composition is a feedstock for utilization within an active drying process (for example, a spray drying feedstock). In other embodiments, a synthetic composition is a feedstock composition for a passive drying process (for example, air drying). In some embodiments, any feedstock described herein may comprise: one or more microorganism, biological extract, metabolite, synthetic molecule, or combinations thereof. In some embodiments, a treatment formulation comprises components in Table 1, 2, 7, or 8. Atreatment formulation may also comprise one or more flowability aid or seed coating component such as hydrous magnesium silicate, calcium carbonate, diatomite, perlite, talc, or plantability polymer such as Flo Rite (R) (BASF Corporation, Florham Park, New Jersey). A treatment formulation may be packaged in a light and/or moisture resistant package, with or without one or more microorganisms, and with or without one or more desiccant. [0042]In some embodiments, the formulation comprises at least 3% total solids, at least 10% total solids, at least 15% total solids, at least 20% total solids, at least 25% total solids, at least 30% total solids, at least 35% total solids, at least 40% total solids, or at least 45% total solids, less than 60% total solids, less than 50% total solids, less than 45% total solids, less than 40% total solids, less than 35% total solids, less than 30% total solids, less than 25% total solids, less than 20% total solids, between about 10% and 60% total solids, between about 20% and 60% total solids, between about 20% and 50% total solids, between about 20% and 40% total solids, or between about 25% and 35% total solids.

WO 2024/168313 PCT/US2024/015273 id="p-43" id="p-43"

[0043]In some embodiments, exemplary compositions include the components as listed in Tables 1and 2.

Table 1. Exemplary feedstock compositions (liquid) % by wet weight.

Feedstock compositions (liquid) % by wet weightIngredients F27 F31 F32 F34maltodextrin — high DE (~18) 13maltodextrin — mid DE (~10) 13 13maltodextrin — low DE (~4) 13peptone 6 5 6 6casamino acids 1.5sorbitolascorbic acid 1.5 1.5 1.5 3trehalose 2 1.5sodium bicarbonate 0.7 0.7 0.7 1.5cysteine 2.5kaolin 10 10 10 10Microbial concentrate 30 30 30 30Water 38.8 34.3 37.3 34.0 Table 2. Exemplary formulation composition, by % dry weight of formulation and by dry weight of ingredient relative to dry microbial biomass.

Formulation composition, by % dry' weight of formulationFormulation composition, by dry weight relative to dry microbial biomassIngredients F27 F31 F32 F34 F27 F31 F32 F34maltodextrin — high DE (~18) 34.4 6.2maltodextrin — mid DE (~10) 39 34.1 6.2 6.2maltodextrin — low DE (~4) 37.4 6.2peptone 18 13.2 17.2 15.7 2.9 2.4 2.9 2.9casamino acids 4 0.7sorbitol 5.3 1.0ascorbic acid 4.5 4 4.3 7.9 0.7 0.7 0.7 1.4trehalose 5.3 4.3 1.0 0.7sodium bicarbonate 2.1 1.9 2 3.9 0.3 0.3 0.3 0.7cysteine 6.6 1.2kaolin 30 26.5 28.7 26.2 4.8 4.8 4.8 4.8Microbial concentrate 6.3 5.6 6.0 5.5 1 1 1 1 WO 2024/168313 PCT/US2024/015273 id="p-44" id="p-44"

[0044]The spray drying feedstock may be prepared as described herein (for example see, FTG. I and Example 4). The order within in which feedstock components are mixed will not affect survival and/or stability of the microbial concentrate as long as the acidic and alkaline components are mixed and pH checked and adjusted to 6.5-7.2 (at least once) before pH sensitive microorganisms are added. For many organisms pH adjustment to 6.5 - 7.2 is important to be performed before addition of the microbial concentrate to prevent negative impacts to cell health and viability. Mixing of the other components may be performed in any order. Mixing of components that will release CO2 and bubble, such as sodium bicarbonate and ascorbic acid, may be performed before mixing of other components. The microbial concentrate may represent any amount of concentration, or no concentration, of microbial biomass (for example, relative to the microbial biomass in growth, production, or extraction media). [0045]The spray dryer is prepared for drying, including selection of nozzles, pump speed/feed rate (kg feedstock per hour), atomization pressure, aspirator air flow %, arrangement for conveying the powder away from the heat source. Nozzles include but are not limited to a rotary disk nozzle, a two fluid nozzle, a single fluid nozzle. The average particle size will depend on the nozzle and pressure used, and one of skill may use standard knowledge to adjust for an average particle size of about 10 /zm to 200 /zm, from about 10 /zm to 50 /zm, 25 /zm to 200 /zm, 25 /zm to 100 /zm, 50 /zm to 100 /zm, 50 /zm to 200 /zm, 100 /zm to 200 /zm, 150 /zm to 250 /zm, 100 /zm to 500 /zm, 200 /zm to 500 /zm, greater than 10 /zm, greater than 20 /zm, greater than 30 /zm, greater than 40 /zm, greater than 50 /zm, greater than 60 /zm, greater than 70 /zm, greater than 80 /zm, greater than 90 /zm, greater than 100 /zm, greater than 110 /zm, greater than 120 /zm, greater than 130 /zm, greater than 140 /zm, greater than 150 /zm, greater than 160 /zm, greater than 170 /zm, greater than 180 /zm, greater than 190 /zm, or greater than 200 /zm, greater than 250 /zm, less than 500 /zm, less than 400 /zm, less than 300 /zm, less than 200 /zm, or less than 100 /zm. [0046]In some embodiments, the inlet temperature is set to 65 degrees Cor greater, 70 degrees C or greater, 75 degrees C or greater, 80 degrees C or greater, 85 degrees C or greater, degrees C or greater, 95 degrees C or greater, 100 degrees C or greater, 105 degrees C or greater, 110 degrees C or greater, 115 degrees C or greater, 120 degrees C or greater, 125 degrees C or greater, 130 degrees C or greater, or 140 degrees C or greater. In some embodiment, the inlet temperature may be set to less than 170 degrees C, less than 165 degrees C, less than 160 degrees C, less than 155 degrees C, less than 150 degrees, less than 145 degrees C, less than 140 degrees WO 2024/168313 PCT/US2024/015273 C, less than 135 degrees C, less than 130 degrees C, less than 125 degrees C, less than 1degrees C, less than 115 degrees C, less than 110 degrees C, less than 100 degrees C, less than degrees C, less than 90 degrees C, less than 85 degrees C, less than 80 degrees C, less than degrees C, or less than 70 degrees C. In some embodiment, the inlet temperature may be set to between 65 degrees C and 140 degrees C, between 70 degrees C and 140 degrees C, between degrees C and 140 degrees C, 80 degrees C and 140 degrees C, between 85 degrees C and 1degrees C, 90 degrees C and 140 degrees C, 95 degrees C and 140 degrees C, or 100 degrees C and 140 degrees C. [0047]In some embodiments, the outlet temperature is be about 5 degrees Clower than the inlet temperature, about 10 degrees C lower than the inlet temperature, about 15 degrees C lower than the inlet temperature, about 20 degrees C lower than the inlet temperature, about 25 degrees C lower than the inlet temperature, about 30 degrees C lower than the inlet temperature, about degrees C lower than the inlet temperature, about 40 degrees C lower than the inlet temperature, about 45 degrees C lower than the inlet temperature, or about 50 degrees C lower than the inlet temperature. [0048]In some embodiments, the outlet temperature is 40 degrees or greater, 45 degrees Cor greater, 50 degrees C or greater, 55 degrees C or greater, 60 degrees C or greater, 65 degrees C or greater, 70 degrees C or greater, 75 degrees C or greater, or 80 degrees C or greater. In some embodiment, the outlet temperature may be set to less than 70 degrees C, less than 65 degrees C, less than 60 degrees C, less than 55 degrees C, less than 50 degrees, or less than 45 degrees C. In some embodiment, the outlet temperature may be set to between 45 degrees C and 65 degrees C, between 50 degrees C and 60 degrees C, between 60 degrees C and 70 degrees C, 60 degrees C and 80 degrees C, between 50 degrees C and 70 degrees C, or between 40 degrees C and degrees C. [0049]For many applications, sufficiently dried spray dried powder will have water activity of less than 0.3. [0050]In some embodiments, the synthetic composition comprises one or more Gram-negative bacteria, one or more non-spore forming Gram-positive bacteria, one or more spore forming Gram-positive bacteria, or fungi. In some embodiments, the synthetic composition comprises one or more microorganisms sensitive to stress conditions, for example environmental conditions WO 2024/168313 PCT/US2024/015273 including temperature, long-term storage, starvation, desiccation, and environmental variability, etc. [0051]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum or division selected from Pseudomonadota, Bacillota, Actinomycetota, Ascomycota, Basidiomycota, Mucoromycota, Bacteroidota, or Proteobatceria. In some embodiments, the synthetic composition comprises one or more microorganism of the class Gammaproteobacteria, Bacilli, Actinomycetia, Alphaproteobacteria, Sordariomycetes, Chitinophagia, Cystobasidiomycetes, or Belaproleobacteria. In some embodiments, the synthetic composition comprises one or more microorganism of the order Bacillales, Caryophanales, Hypocreales, Streptomycetales, Enter obacterales, Micrococcales, Lactobacillales, Rhizobiales, Bifidobacteriales, Kitasatosporales, Coniochaetales, Pseudomonadales, Xanthomonadales, Chitinophagales, Cystobasidiales, Burkholderiales, Rhodospirillales, Sphingomonadales, or Hyphomicrobiales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Alcaligenaceae, Burkholder iaceae, Cellulomonadaceae, Comamonadaceae, Rhizobiaceae, Bacillaceae, PaenibacilIaceae, Hypocreaceae, Streptomycetaceae, Enterobacteriaceae, Mier obacter iaceae, Brucellaceae, LactobacilIaceae, Coniochaetaceae, Pseudomonadaceae, Xanthomonadaceae, Chitinophagaceae, Cystobasidiaceae, Oxalobacteraceae, Azospirillaceae, Sphingomonadaceae, Streptomycetaceae, Erwiniaceae, Bifidobacteriaceae, Nitrobacteraceae, or Xanthobacteraceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Streptomyces, Kosakonia, Curtobacterium, Ochrobacter, Coniochaeta, Pseudomonas, Stenotrophomonas, Rahnella, Occultifur, Herbaspirillum, Azospirillum, Siccibacter, Sphingomonas, Pantoea, Sinorhizobium, Rhizobium, Neorhizobiwn, Bradyrhizobium, Azorhizobium, Lactobacillus, Bifidobacterium or Enterobacter. [0052]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Pseudomonadota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Gammaproteobacteria, Alphaproteobacteria, and/or Betaproteobacteria. In some embodiments, the synthetic composition comprises one or more microorganism of the order Pseudomonadales, Rhizobiales, Sphingomonadales, Hyphomicrobiales, Burkholderiales, Xanthomonadales, and/or Rhodospirillales. In some embodiments, the synthetic composition comprises one or more microorganism of the family WO 2024/168313 PCT/US2024/015273 Pseudomonadaceae, Xanthomonadaceae, Brucellaceae, Sphingomonadaceae, Nitrobacteraceae, Bur kholderiace ae, Oxalobacteraceae, Azospirillaceae, Comamonadaceae, Rhizobiaceae, Alcaligenaceae, and/or Xanthobacteraceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Pseudomonas, Xanthomonas, Ochrobacter, Sphingomonas, Tardiphaga, Sphingomonas, Paraburkholderia, Herbaspirillum, Azospirillum, Variovorax, Agrobacterium, Achromobacter, Rhizobium, and/or Bradyrhizobium. In some embodiments, the synthetic composition comprises one or more microorganism of the species growp Pseudomonas putida. In some embodiments, the synthetic composition comprises one or more microorganism of the species Xanthomonas campestris, Pseudomonas denitrificans, Pseudomonas aegrilactucae, Pseudomonas alloputida, Pseudomonas ceruminis, Pseudomonas faucium, Pseudomonas fulva, Pseudomonas huaxiensis, Pseudomonas inefficax, Pseudomonas monteilii, Pseudomonas mosselii, Pseudomonas oryzihabitans, Pseudomonas parasichuanensis. Pseudomonas persica, Pseudomonas plecoglossicida, Pseudomonas putida, Pseudomonas reidholzensis, Pseudomonas shirazica, Pseudomonas syncyanea, Pseudomonas urethralis, Pseudomonas wadenswilerensis, Azospirillum baldaniorum, Azospirillum brasilense, Bradyrhizobium diversitatis, Herbaspirillum seropedicae, Kosakonia cowanii, Ochrobacter anthropi, Pantoea eucalypti, Rahnella aceris, Siccibacter colletis, Sphingomonas yabuuchiae, and/or Stenotrophomonaspavanii. In some embodiments, the synthetic composition comprises one or more microorganism having one or more polynucleotide sequences at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identical to SEQ ID NO. 5-57, 114-122. In some embodiments, the synthetic composition comprises one or more microorganism having one or more polynucleotide sequences at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identical to SEQ ID NO. 5-57, 114-122. In some embodiments, the synthetic composition comprises one or more microorganism having one or more genes encoding a protein whose amino acid sequence is selected from the group consisting of SEQ ID NOs. 58-113, or combinations thereof. [0053]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Ascomycota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Saccharomycetes, Sordariomycetes, Dothideomycetes, and/or Eurotiomycetes. In some embodiments, the synthetic composition comprises one or more microorganism of the order Hypocreales, Saccharomycetales, Microascales, Eurotial.es, WO 2024/168313 PCT/US2024/015273 Onygenales, Helotiales, Pleosporales, and/or Xylariales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Pichiaceae, Hypocreaceae, Microascaceae, Ophiocordycipitaceae, Aspergillaceae, Saccharomycetaceae, Gymnoascaceae, Thermoascaceae, Debaryomycetaceae, Helotiaceae, Dipodascaceae, and/or Xylariaceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Ogataea, Geotrichum, Brettanomyces, Yarrowia, Debaryomyces, Saccharomyces, Trichoderma, Monascus, Acremonium, Aspergillus, Tolypocladium, Gymnoascus, Hypomyces, Glarea, Eupenicillium, Nakaseomyces, Meyerozyma, and/dr Nodulisporium. In some embodiments, the synthetic composition comprises one or more microorganism of the species Yarrowia lipolytica, Nakaseomyces glabratus (Candida glabrata), Candida famata (Debaryomyces hansenii), Meyerozyma guilliermondii (Candida guilliermondii), Acremonium eqyptiacum, Saccharomyces cerevisiae, Glarea lozoyensis, Tolypocladium nivenum, Ogataea glucozyma (Pichia glucozyma), and/or Nodulisporium sylviforme. [0054]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Actinomycetota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Actinomycetes. In some embodiments, the synthetic composition comprises one or more microorganism of the order Propionibacteriales, Micrococcales, Bifidobacteriales, and or Streptomycetales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Propionibacteriaceae, Bijidobacteriaceae, Microbacteriaceae, Brevibacteriaceae, Streptomycetaceae, and/or Cellulomonadaceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Propionibacterium, Kocuria, Curtobacterium, Brevibacterium, Streptomyces, and/or Cellulomonas. In some embodiments, the synthetic composition comprises one or more microorganism of the species Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum, Micrococcus roseus (Kocuria rosea), Propionibacterium freudenreichii, Curtobacterium gossypii, Curtobacterium lutem, Curtobacterium pusilium, Cellulomonas humilata, Streptomyces ciscaucasicus, Streptomyces kathirae, Streptomyces canus, Streptomyces gardneri, Streptomyces phaeoluteigriseus, Streptomyces virginiae, Streptomyces lydicus, Streptomyces atrovirens, Streptomyces griseovirdis, Streptomyces olivaceoviridis, Streptomyces rimosus, Streptomyces rochet, Streptomyces viridis, Streptomyces igroscopicus, Streptomyces carpinensis, Streptomyces thermolilacinus, Streptomyces hygroscopicus, Streptomyces WO 2024/168313 PCT/US2024/015273 jilipinensis, Streptomyces yokosukaensis, Streptomyces roseochromogenes, Streptomyces carbophilus, Streptomyces tsukubaensis, Streptomyces chrestomyceticus, Brevibacterium linens, and/or Streptomyces spiralis. [0055]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Bacillota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Bacilli and/or Clostridia. In some embodiments, the synthetic composition comprises one or more microorganism of the order Bacillales, Eubacteriales, Lactobacillales, and/or Cary ophanales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Bacillaceae, Enterococcaceae, Streptococcaceae, Lactobacillaceae, Oscillospiraceae, and/or Paenibacillaceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Bacillus, Lactococcus, Leuconostoc, Faecalibacterium, Oenococcus, Paenibacillus, Pediococcus, Priestia, Lactobacillus, Streptococcus, Weissella, and/or Peribacillus. In some embodiments, the synthetic composition comprises one or more microorganism of the species Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus velezensis, Peribacillus simplex, Paenibacillus taichungensis, Bacillus stratosphericus, Bacillus megaterium, Peribacillus butanolivorans, Bacillus aryabhatti, Bacillus haloterans, Bacillus toyonensis, Bacillus safensis, Lactobacillus delbreuckii, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus lactis, and^r Lactobacillus Reuteri. [0056]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Bacteroidota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Bacteroidia and/or Chitinophagia. In some embodiments, the synthetic composition comprises one or more microorganism of the order Bacteroidales and/or Chitinophagales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Bacteroidaceae and/or Chitinophagaceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Bacteroides and/or Chitinophaga. In some embodiments, the synthetic composition comprises one or more microorganism of the species Bacteroides ovatus. Chitinophaga sancti, and/or Chitinophaga ginsengisegetis.

WO 2024/168313 PCT/US2024/015273 id="p-57" id="p-57"

[0057]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Mucoromycota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Mucoromycetes. In some embodiments, the synthetic composition comprises one or more microorganism of the order Mucorales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Rhizopodaceae and/or Choanephoraceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Rhizopus and/or Blakeslea. In some embodiments, the synthetic composition comprises one or more microorganism of the species Rhizopus oryzae (Rhizopus arrhizus) and/or Blakeslea trispora (Choanephora trispora). [0058]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Basidiomycota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Cystobasidiomycetes, Agaricostilbomycetes, Tremellomycetes, and/or Agaricomycetes. In some embodiments, the synthetic composition comprises one or more microorganism of the order Agaricostilbales, Tremellales, Cystobasidiales, Cystofilobasidiales, or Polypor ales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Agaricostilbaceae, Sirobasidiaceae, Cystobasidiaceae, Mrakiaceae, and/or Meruliaceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Sirobasidium, Occultifur, Taxomyces, Cer iporiopsis, and/or Phaffia. In some embodiments, the synthetic composition comprises one or more microorganism of the species Occultifur kilbournensis, Phaffia rhodozyma (Xanthophyllomyces dendrorhous), and/or Taxomyces andreanae (Ceriporiopsis andreanae). [0059]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Proteobatceria. In some embodiments, the synthetic composition comprises one or more microorganism of the class Gammaproteobacteria. In some embodiments, the synthetic composition comprises one or more microorganism of the order Enterobacterales. In some embodiments, the synthetic composition comprises one or more microorganism of the family Enterobacteriaceae. In some embodiments, the synthetic composition comprises one or more microorganism of the genus Rahnella. In some embodiments, the synthetic composition comprises one or more microorganism of the species Rahnella aceris. [0060]In some embodiments, the synthetic composition comprises one or more microorganism of the phylum Peploviricota, Negarnaviricota, Cossaviricota, Kitrinoviricota, Preplasmiviricota, WO 2024/168313 PCT/US2024/015273 andor Nucleocytoviricota. In some embodiments, the synthetic composition comprises one or more microorganism of the class Herviviricetes, Papovaviricetes, Flasuviricetes, Tectiliviricetes, Insthoviricetes, and/or Pokkesviricetes. In some embodiments, the synthetic composition comprises one or more microorganism of the order Herpesvirales, Articulavirales, Amarillovirales, Zurhausenvirales, Rowavirales, and/or Chitovirales. [0061]In some embodiments, the synthetic composition comprises one or more of a surfactant, a buffer, a tackifier, a microbial stabilizer, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, and a polymer. In some embodiments, the polymer is a biodegradable polymer selected from the group consisting of alginate, agarose, agar, gelatin, polyacrylamide, chitosan, polyvinyl alcohol, and combinations thereof. In some embodiments, the biodegradable polymer is alginate, and the alginate is sodium alginate. [0062]In some embodiments, the synthetic composition comprises one or more microorganisms and one or chemical or biological agent capable of capable of killing, impeding the feeding and/or growth and/or reproduction of, repelling, and/or reducing the severity or extent of infection to a plant or animal host of, a pest of a plant or animal, including without limitation chemical or biological agents that are acetylcholinesterase (AChE) inhibitors, GABA-gated chloride channel blockers, sodium channel modulators, nicotinic acetylcholine receptor (nAChR) competitive modulators, nicotinic acetylcholine receptor (nAChR) allosteric modulators - Site 1, Glutamate-gated chloride channel (GluCl) allosteric modulators, Chordotonal organ TRPV channel modulators, Nicotinic acetylcholine receptor (nAChR) channel blockers, Octopamine receptor agonists, Voltage-dependent sodium channel blockers, multi-site inhibitors, Ryanodine receptor modulators, chordotonal organ modulators (wherein the chordotonal organ modulator does not bind to the Nan-lav TRPV channel complex), GABA-gated chloride channel allosteric modulators, GABA-gated chloride channel allosteric modulators - Site II, nicotinic acetylcholine receptor (nAChR) Allosteric Modulators - Site II, Juvenile hormone mimics, Mite growth inhibitors affecting CHS1, Inhibitors of chitin biosynthesis affecting CHS1, Inhibitors of chitin biosynthesis - type 1, Molting disruptors - Dipteran, Ecdysone receptor agonists, Inhibitors of acetyl CoA carboxylase, Inhibitors of mitochondrial ATP synthase, Uncouplers of oxidative phosphorylation via disruption of the proton gradient, Mitochondrial complex III electron transport inhibitors, Mitochondrial complex I electron transport inhibitors, Mitochondrial WO 2024/168313 PCT/US2024/015273 complex IV electron transport inhibitors, Mitochondrial complex II electron transport inhibitors, Microbial disruptors of insect midgut membranes, Host-specific occluded pathogenic viruses, other active compounds (such as Azadirachtin, Benzoximate, Bromopropylate, Chinomethionat, Dicofol, Lime sulfur, Mancozeb, Pyridalyl, Sulfur, Chlorantraniliprole, Clothianidin, Tioxazafen, Fluopyram), other active bacterial agents (such as certain Burkholderia strains including without limitation Burkholderia rinojenses, Wolbachiapipientis), other active fungal agents (such as Beauveria bassiana strains, Metarhizium anisopliae strain F52, Paecilomyces fumosoroseus Apopka strain 97), biological essence including synthetics or extracts or refined or unrefined oils (such as Dysphania ambrosioides near ambrosioides extract, fatty acid monoesters with glycerol or propanediol, neem oil), non-specific mechanical disruptors (such as Diatomaceous earth), or combinations thereof. Examples of AchE inhibitors include without limitation Carbamates (such as Alanycarb, Aldicarb, Bendiocarb, Benfuracarb, Butocarboxim, Butoxycarboxim, Carbaryl, Carbofuran, Carbosulfan, Ethiofencarb, Fenobucarb, Formetanate, Furathiocarb, Isoprocarb, Methiocarb, Methomyl, Metolcarb, Oxamyl, Pirimicarb, Propoxur, Thiodicarb, Thiofanox, Triazamate,Trimethacarb, XMC, Xylylcarb) and Organophosphates (such as Acephate, Azamethiphos, Azinphos-ethyl, Azinphosmethyl, Cadusafos, Chlorethoxyfos, Chlorfenvinphos, Chlormephos, Chlorpyrifos, Chlorpyrifos-methyl, Coumaphos, Cyanophos, Demeton-S-methyl, Diazinon, Dichlorvos/ DDVP, Dicrotophos, Dimethoate, Dimethylvinphos, Disulfoton, EPN, Ethion, Ethoprophos, Famphur, Fenamiphos, Fenitrothion, Fenthion, Fosthiazate, Heptenophos, Imicyafos, Isofenphos, Isopropyl O-(methoxyaminothio-phosphoryl) salicylate, Isoxathion, Malathion, Mecarbam, Methamidophos, Methidathion, Mevinphos, Monocrotophos, Naled, Omethoate, Oxydemeton-methyl, Parathion, Parathion-methyl, Phenthoate, Phorate, Phosalone, Phosmet, Phosphamidon, Phoxim, Pirimiphos- methyl, Profenofos, Propetamphos, Prothiofos, Pyraclofos, Pyridaphenthion, Quinalphos, Sulfotep, Tebupirimfos, Temephos, Terbufos, Tetrachlorvinphos, Thiometon, Triazophos, Trichlorfon, Vamidothion). Examples of GABA- gated chloride channel blockers include without limitation Cyclodiene Organochlorines (such as Chlordane, Endosulfan) and Phenylpyrazoles (Fiproles) (such as Ethiprole, Fipronil). Examples of sodium channel modulators include without limitation pyrethroids and pyrethrins (such as Acrinathrin, Allethrin, d-cis-trans Allethrin, d-trans Allethrin, Bifenthrin, Bioallethrin, Bioallethrin Scyclopentenyl isomer , Bioresmethrin, Cycloprothrin, Cyfluthrin, beta-Cyfluthrin, Cyhalothrin, lambda-Cyhalothrin, gamma-Cyhalothrin, Cypermethrin, alpha-Cypermethrin, beta- WO 2024/168313 PCT/US2024/015273 Cypermethrin, thetacypermethrin, zeta-Cypermethrin, Cyphenothrin, (IR)-trans- isomers], Deltamethrin, Empenthrin (EZ)-(IR)- isomers], Esfenvalerate, Etofenprox, Fenpropathrin, Fenvalerate, Fluey thrinate, Flumethrin, tau-Fluvalinate, Halfenprox, Imiprothrin, Kadethrin, Permethrin, Phenothrin [(IR)-trans- isomer], Prallethrin, Pyrethrins (pyrethrum), Resmethrin, Silafluofen, Tefluthrin, Tetramethrin, Tetramethrin [(IR)-isomers], Tralomethrin, Transfluthrin) and Methoxychlor. Examples of nAChR competitive modulators include without limitation Neonicotinoids (such as Acetamiprid, Clothianidin, Dinotefuran, Imidacloprid, Nitenpyram, Thiacloprid, Thiamethoxam), nicotine, sulfoximines (such as Sulfoxaflor), Butenolides (such as Flupyradifurone), and Mesoionics (such as Triflumezopyrim). Examples of nAChR allosteric modulators - Site I include without limitation Spinosyns (such as Spinetoram, Spinosad).Examples of GluCl allosteric modulators include without limitation Avermectins and Milbemycins (such as Abamectin, Emamectin benzoate, Lepimectin, Milbemectin). Examples of mult-site inhibitors include without limitation Alkyl halides (such as Methyl bromide and other alkyl halides), Chloropicrin, Fluorides (such as Cryolite (Sodium aluminum fluoride), Sulfuryl fluoride), Borates (such as Borax, Boric acid, Disodium octaborate, Sodium borate, Sodium metaborate), Tartar emetic, Methyl isothiocyanate generators (such as Dazomet, Metam).Examples of chordotonal organ TRPV channel modulators include without limitation Pyridine azomethine derivatives (such as Pymetrozine, Pyrifluquinazon), and Pyropenes (such as Afidopyropen). Examples of juvenile hormone mimics include without limitation juvenile hormone analogues (such as Hydroprene, Kinoprene, Methoprene), fenoxycarb, and pyriproxyfen. Examples of mite growth inhibitors affecting CHSI include without limitation Clofentezine, Diflovidazin, Hexythiazox, and Etoxazole. Examples of microbial disruptors of insect midgut membranes include without limitation Bacillus thuringiensis (such as Bacillus thuringiensis subsp. Israelensis, Bacillus thuringiensis subsp. Aizawai, Bacillus thuringiensis subsp. Kurstaki, Bacillus thuringiensis subsp. Tenebrionis, Bacillus thuringiensis strain EX297512') and the insecticidal proteins they produce (such as Cryl Ab, Cryl Ac, CrylFa, CrylA.105, Cry2Ab, Vip3A, mCry3A, Cry3Ab, Cry3Bb, Cry34Abl/Cry35Abl) and Bacillus sphaericus. Examples of inhibitors of mitochondrial ATP synthase include without limitation Diafenthiuron, Organotin miticides (such as Azocyclotin, Cyhexatin, Fenbutatin oxide), Propargite, and Tetradifon. Examples of uncouplers of oxidative phosphorylation via disruption of the proton gradient include without limitation Pyrroles (such as Chlorfenapyr), WO 2024/168313 PCT/US2024/015273 Dinitrophenols, and Sulfluramid. Examples of nAChR channel blockers include without limitation Nereistoxin analogues (such as Bensultap, Cartap hydrochloride, Thiocyclam, Thiosultap-sodium). Examples of inhibitors of chitin biosynthesis affecting CHSI include without limitation Benzoylureas (such as Bistrifluron, Chlorfluazuron, Diflubenzuron, Flucycloxuron, Flufenoxuron, Hexaflumuron, Lufenuron, Novaluron, Noviflumuron, Teflubenzuron, Triflumuron). Examples of inhibitors of chitin biosynthesis - type 1 include without limitation Buprofezin. Examples of molting disruptors (Dipteran) include without limitation Cyromazine. Examples of ecdysone receptor agonists include without limitation Diacylhydrazines (such as Chromafenozide, Halofenozide, Methoxyfenozide, Tebufenozide). Examples of octopamine receptor agonists include without limitation Amitraz. Examples of mitochondrial complex III electron transport inhibitors include without limitation Hydramethylnon, Acequinocyl, Fluacrypyrim, and Bifenazate. Examples of mitochondrial complex I electron transport inhibitors include without limitation METI acaricides and insecticides such as Fenazaquin, Fenpyroximate, Pyridaben, Pyrimidifen, Tebufenpyrad, Tolfenpyrad) and Rotenone. Examples of voltage-dependent sodium channel blockers include without limitation Oxadiazines (such as Indoxacarb) and Semicarbazones (such as Metaflumizone). Examples of inhibitors of acetyl CoA carboxylase include without limitation Tetronic and Tetramic acid derivatives (such as Spirodiclofen, Spiromesifen, Spiropidion, Spirotetramat). Examples of mitochondrial complex IV electron transport inhibitors include without limitation Phosphides (Aluminium phosphide, Calcium phosphide, Phosphine, Zinc phosphide), Cyanides (such as Calcium cyanide, Potassium cyanide, Sodium cyanide). Examples of mitochondrial complex II electron transport inhibitors include without limitation Beta- ketonitrile derivatives (such as Cyenopyrafen, Cyflumetofen) and Carboxanilides (such as Pyflubumide). Examples of ryanodine receptor modulators include without limitation such as Diamides (such as Chlorantraniliprole, Cyantraniliprole, Cyclaniliprole Flubendiamide, Tetraniliprole). Examples of chordotonal organ modulators include without limitation Flonicamid. Examples of GABA-gated chloride channel allosteric modulators include without limitation Meta-diamides (Broflanilide) and Isoxazolines (such as Fluxametamide). Examples of nicotinic acetylcholine receptor (nAChR) Allosteric Modulators - Site II include without limitation GS-omega/kappaHXTX-Hvla peptide.

WO 2024/168313 PCT/US2024/015273 id="p-63" id="p-63"

[0063]In some embodiments, the synthetic composition comprises one or more microorganisms and one or chemical or biological agent capable of killing, impeding the feeding and/or growth and/or reproduction of, repelling, and/or reducing the severity or extent of infection to a plant host of, an pathogen of a plant, including without limitation chemical or biological agents that are PhenylAmides fungicides (acylalanines, oxazolidinones, butyrolactones), hydroxy-(2-amino- ) pyrimidines, heteroaromatics (such as isoxazoles, isothiazolones), carboxylic acids, Methy- Benzimidazole-Carbamates (MBC) fungicides (such as thiophanates, benzimidazoles), N-phenyl carbamates, benzamides (such as toluamides, pyridinylmethyl-benzamides), thiazole carboxamide (such as ethylamino-thiazole-carboxamide), phenylureas, cyanoacrylates (such as aminocyanoacrylates), aryl-phenyl-ketones (such as benzophenone, benzoylpyridine), pyrimidinamines, pyrazole-METl (such as pyrazole-5-carboxamides), quinazoline, succinate- dehydrogenase inhibitors (SDHI) (such as phenyl-benzamides, phenyl-oxo-ethyl thiophene amide, pyridinyl-ethyl-benzamides, phenyl-cyclobutyl-pyridineamide, furan- carboxamides, oxathiin- carboxamides, thiazole- carboxamides, pyrazole-4- carboxamides, N-cyclopropyl-N- benzyl-pyrazole-carboxamides, N-methoxy-(phenyl-ethyl)-pyrazole-carboxamides, pyridine- carboxamides, pyrazine-carboxamides, pydiflumetofen, fluxapyroxad), quinone outside inhibitors (such as methoxy-acrylates, methoxy-acetamide, methoxy-carbamates, oximino- acetates, oximino-acetamides, oxazolidine-diones, dihydro-dioxazines, imidazolinones, benzyl- carbamates, tetrazolinones), quinone inside inhibitors (such as cyano-imidazole, sulfamoyl- triazole, picolinamides), uncouplers of oxidative phosphorylation (such as dinitrophenyl- crotonates, 2,6-dinitro-anilines), organo tin compounds (tri-phenyl tin compounds), thiophene- carboxamides, Quinone outside Inhibitor - stigmatellin binding type (such as triazolo- pyrimidylamine), anilino-pyrimidines, enopyranuronic acid antibiotic, hexopyranosyl antibiotic, glucopyranosyl antibiotic, tetracycline antibiotic, aza-naphthalenes (such as aryloxyquinoline, quinazolinone), phenylpyrroles, dicarboximides, phosphoro-thiolates, dithiolanes, aromatic hydrocarbons, chlorophenyls, nitroanilines, heteroaromatics (such as 1,2,4-thiadiazoles), carbamates, demethylation inhibitors (such as piperazines, pyridines, pyrimidines, imidazoles, tri azoles, triazolinthiones), amines (such as morpholines, piperidines, spiroketal-amines), ketoreductase inhibitors (such as hydroxyanilides, amino-pyrazolinone), thiocarbamates, allylamines, polyoxins (such as peptidyl pyrimidine nucleoside), Carboxylic Acid Amides (such as cinnamic acid amides, valinamide carbamates, mandelic acid amides), melanin biosynthesis WO 2024/168313 PCT/US2024/015273 inhibitors - reductase (such as isobenzo-furanone, pyrrolo-quinolinone, tri azol obenzo-thi azole), melanin biosynthesis inhibitors - dehydratase (such as cyclopropane-carboxamide, carboxamide, propionamide), melanin biosynthesis inhibitors - polyketide synthase (such as trifluoroethyl- carbamate), benzo-thiadi azole, benzisothiazole, thiadiazole-carboxamide, polysaccharides (such as laminarin), plant ethanol extracts (such as anthraquinones, resveratrol, extract from Reynoutria aralinensis phosphonates (such as ethyl phosphonates, fosetyl-Al, phosphorous acid and salts), isothiazole (such as isothiazolylmethyl ether), cyanoacetamide-oxime, phthalamic acids, benzotriazines, benzene-sulphonamides, pyridazinones, phenyl-acetamide, guanidines, thiazolidine (such as cyano-methylene-thiazolidines), pyrimidinone-hydrazones, 4-quinolyl- acetates, tetrazolyloximes, glucopyranosyl antibiotics, copper salts, sulphur, dithio-carbamates and relatives (such as amobam, ferbam, mancozeb, maneb, metiram, propineb, thiram, zinc thiazole, zineb, ziram), phthalimides, chloronitriles (phthalonitriles), sulfamides (such as dichlofluanid, tolylfluanid), bis-guanidines (such as guazatine, iminoctadine), triazines (such as anilazine), quinones (anthraquinones) (such as dithianon), quinoxalines (such as chinomethionat, quinomethionate), maleimide (such as fluoroimide), thiocarbamate (such as methasulfocarb), polypeptide (lectin) plant extracts (such as extract from the cotyledons of lupine plantlets), phenol and sesquiterpene and triterpenoid and coumarin plant extracts (such as extract from Swinglea glutinosa), terpene hydrocarbon and terpene alcohol and terpene phenol extracts plant extracts (such as extract from Melaleuca altemifolia, plant oils such as eugenol, geraniol, thymol mixtures thereof), Polyene (such as amphoteric macrolide antifungal antibiotic from Streptomyces natalensis or Streptomyces chattanoogensis}, oxysterol binding protein homologue inhibition (piperidinyl-thiazole-isoxazolines), other active compounds (such as Fludioxonil, Mefenoxam, Sedaxane, Azoxystrobin, Thiabendazole, Ethaboxam, metalaxyl, Trifloxystrobin, Myclobutanil, Acibenzolar-S-methyl, Metconazole, tolclofos-methyl, Fluopyram, Ipconazole, Oxathiapiprolin, Difenoconazole, Prothyoconazol, Tebuconazole, Pyraclostrobin, Fluxapyroxad), and combinations thereof. [0064]In some embodiments, the synthetic composition comprises one or more microorganisms capable of killing, impeding the feeding and/or growth and/or reproduction of, repelling, and/or reducing the severity or extent of infection to a plant host of, a pathogen or pest of a plant. Non- limiting examples of biological agents include Trichoderma species including without limitation Trichoderma atroviride strain 1-1237, Trichoderma atroviride strain LU 13 2, Trichoderma WO 2024/168313 PCT/US2024/015273 atroviride strain SCI, Trichoderma atroviride strain SKT-1, Trichoderma atroviride strain 77B, Trichoderma asperellum strain T34, Trichoderma asperellum strain kd, Trichoderma harzianum strain T-22, Trichoderma virens strain G-41; Clonostachys species including without limitation Gliocladium catenulatum strain J1446, Clonostachys rosea strain CR-7; Coniothyrium species including without limitation Coniothyrium minitans strain CON/M/91-08; Talaromyces species including without limitation Talaromycesflavus strain SAY-Y-94-01; Saccharomyces species including without limitation Saccharomyces cerevisae strain LAS02׳ , Bacillus species including without limitation Bacillus amyloliquefaciens strain QST713, Bacillus amyloliquefaciens strain FZB24, Bacillus amyloliquefaciens strain MB1600, Bacillus amyloliquefaciens strain D747, Bacillus amyloliquefaciens strain F727, Bacillus amyloliquefaciens strain AT-332, Bacillus amyloliquefaciens strain MB I 600 Bacillus mycoides isolate J, Bacillus subtilis strain AFS032321, Bacillus subtilis strain ¥1336, Bacillus subtilis strain HAI-0404); Pseudomonas species including without limitation Pseudomonas chlororaphis strain AFS009; Streptomyces species including without limitation Streptomyces griseovirides strain K61, Streptomyces lydicus strain WYEC108׳ , Penicillium species such as Penicillium hilaiae. Penicillium hilaiae; Pasteuria species including without limitation Pasteuria nishizawae Pnlf [0065]In some embodiments, a synthetic composition of the present invention comprises a combination of a microorganism, treatment formulation, and fertilizer. In some embodiments, the combination of the microorganism and treatment formulation are present in a concentration of the combination on a fertilizer (as percent by weight) of at least 0.01%, between 0.01% and 0.04%, at least 0.04%, between 0.04% and 0.1%, at least 0.1%, between 0.1% and 0.4%, or at least 0.4%. [0066]In some embodiments, the synthetic composition may be stored at between 0 degrees Celsius and 4 degrees Celsius for 1 week with less than 1 log loss of CFU of the one or more microorganisms. In some embodiments, the synthetic composition may be stored at between 4.degrees Celsius and 20 degrees Celsius for 1 week with less than 1 log loss of CFU of the one or more microorganisms. In some embodiments, the synthetic composition may be stored at between 20.1 degrees Celsius and 33 degrees Celsius for 1 week with less than 1 log loss of CFU of the one or more microorganisms. In some embodiments, the synthetic composition may be stored at between 20 degrees Celsius and 33 degrees Celsius for 28 days, with less than 2 log loss of CFU of the one or more microorganisms. In some embodiments, the synthetic WO 2024/168313 PCT/US2024/015273 composition may be stored at between 20 degrees Celsius and 33 degrees Celsius for between and 100 days, with less than 2 log loss of CPU of the one or more microorganisms. In some embodiments, the synthetic composition may be stored at between 20 degrees Celsius and degrees Celsius for more than 100 days, with less than 2 log loss of CPU of the one or more microorganisms. In some embodiments, the synthetic composition is stored between 20% relative humidity and 80% relative humidity or between 40% relative humidity and 80% relative humidity. In some embodiments, the synthetic composition may be stored at between 20 degrees Celsius and 33 degrees Celsius at between 10% and 50% relative humidity for more than 1days, with less than 2 log loss of CPU of the one or more microorganisms. [0067]In some embodiments, the synthetic composition comprises a plant element. In some embodiments, the plant element is a monocot. In some embodiments, the monocot is a cereal. In some embodiments, the cereal is selected from the group consisting of wheat, rice, barley, buckwheat, rye, millet, oats, corn, sorghum, triticale, and spelt. In some embodiments, the cereal is wheat. In some embodiments, the plant element is a dicot. In some embodiments, the dicot is selected from the group consisting of cotton, tomato, lettuce, peppers, cucumber, endive, melon, potato, and squash. In some embodiments, the dicot is a legume. In some embodiments, the legume is soy, peas, or beans. [0068]In some embodiments, the plant element is a whole plant, seedling, meristematic tissue, ground tissue, vascular tissue, dermal tissue, seed, leaf, root, shoot, stem, flower, fruit, stolon, bulb, tuber, corm, keikis, shoot, or bud. In some embodiments, the plant element is a seed. [0069]In some embodiments, the trait of agronomic importance is improved nutrient use efficiency. In some embodiments, the trait of agronomic importance is drought tolerance. [0070]In some embodiments, the one or more microorganisms comprise at least microorganisms. In some embodiments, the one or more microorganisms comprise at least 3microorganisms. In some embodiments, the one or more microorganisms comprise at least 4microorganisms. In some embodiments, the one or more microorganisms comprise at least 5microorganisms. In some embodiments, the one or more microorganisms comprise at least 10microorganisms. [0071]In some embodiments, the one or more microorganisms are encapsulated in polymeric beads. In some embodiments, the polymeric beads are less than 500 /rm in diameter at their widest point. In some embodiments, the polymeric beads are less than 200 [1m in diameter at WO 2024/168313 PCT/US2024/015273 their widest point. In some embodiments, the polymeric beads are less than 100 /rm in diameter at their widest point. In some embodiments, the polymeric beads are less than 50 /zm in diameter at their widest point. In some embodiments, the polymeric beads ’ average diameter at their widest point is between 500 /rm and 250 /rm. In some embodiments, the polymeric beads ’ average diameter at their widest point is between 249 /rm and 100 /rm. In some embodiments, the polymeric beads ’ average diameter at their widest point is between 100 /rm and 50 /rm. [0072]In some embodiments, the one or more microorganisms are encapsulated in waxes or oils. In some embodiments, the wax or oil encapsulated microorganisms are less than 500 /rm in diameter at their widest point. In some embodiments, the wax or oil encapsulated microorganisms are less than 200 /rm in diameter at their widest point. In some embodiments, the wax or oil encapsulated microorganisms are less than 100 /rm in diameter at their widest point. In some embodiments, the wax or oil encapsulated microorganisms are less than 50 /rm in diameter at their widest point. In some embodiments, the wax or oil encapsulated microorganisms ’ average diameter at their widest point is between 500 /rm and 250 /rm. In some embodiments, the wax or oil encapsulated microorganisms ’ average diameter at their widest point is between 249 /rm and 100 /rm. In some embodiments, the wax or oil encapsulated microorganisms ’ average diameter at their widest point is between 100 /rm and 50 /rm. In some embodiments, encapsulation techniques are spray-drying, spray-chilling, freeze-drying, emulsion-based technique, extrusion-dripping, coacervation, and fluidized-bed-coating. [0073]In one aspect, the disclosure provides a formulation comprising; (a) microbial biomass; (b) one or more antioxidant in a ratio of about 0.07 to 14 by weight relative to the microbial biomass; (c) one or more pH modifier in a ratio of about 0.03 to 7 by weight relative to the microbial biomass; (d) one or more saccharide in a ratio of about 0.07 to 62 by weight relative to the microbial biomass; and (e) one or more protein hydrolysate in a ratio of about 0.07 to 29 by weight relative to the microbial biomass. [0074]In some embodiments, the one or more antioxidant is present in a ratio of at least 0.35 by weight relative to the microbial biomass. In some embodiments, the one or more pH modifier is present in a ratio of at least 0.15 by weight relative to the microbial biomass. In some embodiments, the one or more saccharide is present in a ratio of at least 0.35 by weight relative to the microbial biomass. In some embodiments, the one or more protein hydrolysate is present in a ratio of at least 0.35 by weight relative to the microbial biomass.

WO 2024/168313 PCT/US2024/015273 id="p-75" id="p-75"

[0075]In some embodiments, the one or more antioxidant comprises ascorbic acid and/or creatine. In some embodiments, the one or more amino acid comprises cysteine and/or glutathione. In some embodiments, the one or more pH modifier comprises sodium bicarbonate and/or sodium hydroxide. In some embodiments, the one or more saccharide comprises one or more of maltodextrin, sucrose, lactose, trehalose, and/or microcrystalline cellulose. In some embodiments, the one or more protein hydrolysate comprises one or more of peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein. [0076]In some embodiments, the formulation further comprises at least one inert solid present in a ratio of about 0.48 to 48 by weight relative to the microbial biomass. [0077]In some embodiments, the at least one inert solid comprises kaolin clay, magnesium stearate, or microcrystalline cellulose. [0078]In some embodiments, the one or more saccharide comprises maltodextrin. [0079]In some embodiments, the maltodextrin is present at a concentration of between about 0.62 to 62 by weight relative to microbial biomass. [0080]In some embodiments, the maltodextrin is present at a concentration of at least 3.1 by weight relative to the microbial biomass. [0081]In some embodiments, the one or more saccharide comprises maltodextrin and trehalose. [0082]In some embodiments, the maltodextrin has a dextrose equivalent (DE) value between and 20. In some embodiments, the maltodextrin has a dextrose equivalent (DE) value of at least 8. In some embodiments, wherein the maltodextrin has a dextrose equivalent (DE) value of at least 10. In some embodiments, the maltodextrin has a dextrose equivalent (DE) value of at least 15. [0083]In some embodiments, the formulation further comprises one or more of a sugar alcohol, an amino acid, yeast extract, and/or whey powder. [0084]In some embodiments, the formulation comprises at least one amino acid present at a concentration of between about 0.12 to 12 by dry weight relative to dry microbial biomass. In some embodiments, the at least one amino acid comprises cysteine or glutathione. [0085]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about WO 2024/168313 PCT/US2024/015273 3.1 to 62 by weight relative to the microbial biomass; and the one or more protein hydrolysate comprises peptone in a ratio of about 1.45 to 29 by weight relative to the microbial biomass. [0086]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.3 to 3 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to 62 by weight relative to the microbial biomass; and the one or more protein hydrolysate comprises peptone in a ratio of about 2.9 to 29 by weight relative to the microbial biomass. In some embodiments, the maltodextrin has a dextrose equivalent (DE) value between about 6 and 14. [0087]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass and trehalose present in a ratio of about 0.to 10 by weight relative to the microbial biomass; the one or more protein hydrolysate comprises peptone present in a ratio of about 1.2 to 24 by weight relative to the microbial biomass and casamino acids present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and the formulation additionally comprises sorbitol present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass. [0088]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.3 to 3 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to 62 by weight relative to the microbial biomass and trehalose present in a ratio of about 1 to by weight relative to the microbial biomass; the one or more protein hydrolysate comprises peptone present in a ratio of about 2.4 to 24 by weight relative to the microbial biomass and casamino acids present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; and the formulation additionally comprises sorbitol present in a ratio of about 1 to 10 by weight relative to the microbial biomass. In some embodiments, the maltodextrin has a dextrose equivalent (DE) value of at least 14.

WO 2024/168313 PCT/US2024/015273 id="p-89" id="p-89"

[0089]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass and trehalose present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass. [0090]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.3 to 3 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to 62 by weight relative to the microbial biomass and trehalose present in a ratio of about 0.to 7 by weight relative to the microbial biomass; and the one or more protein hydrolysate comprises peptone present in a ratio of about 2.9 to 29 by weight relative to the microbial biomass. [0091]In some embodiments, the maltodextrin has a dextrose equivalent (DE) value between about 3 and 6. [0092]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to 14 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass; and the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass; and the formulation additionally comprises cysteine present in a ratio of about 0.6 to by weight relative to the microbial biomass. [0093]In some embodiments, the one or more antioxidant comprises ascorbic acid present in a ratio of about 1.4 to 14 by weight relative to the microbial biomass; the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to 62 by weight relative to the microbial biomass; and the one or more protein hydrolysate WO 2024/168313 PCT/US2024/015273 comprises peptone present in a ratio of about 2.9 to 29 by weight relative to the microbial biomass; and the formulation additionally comprises cysteine present in a ratio of about 1.2 to by weight relative to the microbial biomass. [0094]In some embodiments, the maltodextrin has a dextrose equivalent (DE) value between about 6 and 14. [0095]In some embodiments, the formulation further comprises one or more an oil, a wax, an antioxidant, a pH modifier, a surfactant, an adherent, a bulking agent, a solid diluent, a tackifier, a microbial stabilizer, an antimicrobial, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, a polymer, a protein hydrolysate, a plant extract, rheology modifier, a dispersant, an emulsifier, a colorant, a pH buffer, and/or an anticoagulant. [0096]In some embodiments, the weights are wet weights. In some embodiments, the weights are dry weights. [0097]In some embodiments, the microbial biomass comprises a living microorganism, an inactivated microorganism, an attenuated microorganism, a dead microorganism, an extract of a microorganism, and/or a metabolite of a microorganism. [0098]In some embodiments, the microbial biomass comprises a living, non-spore forming microorganism. [0099]In some embodiments, the microbial biomass comprises a Gram-negative bacteria. [00100]In some embodiments, the microbial biomass comprises one or more organism of the phyla/division Pseudomonadota, Bacillota, Actinomycetota, Ascomycota, Basidiomycota, Bacteroidota, Mucoromycota, or Proteobatceria. [00101]In some embodiments, the microbial biomass comprises one or more organism of the family Alcaligenaceae, Agaricostilbaceae, Aspergillaceae, Azospirillaceae, Bacillaceae, Bacteroidaceae, Bifidobacteriaceae, Brevibacteriaceae, Brucellaceae, Burkholder iaceae, Celhdomonadaceae, Chitinophagaceae, Choanephoraceae, Comamonadaceae, Coniochaetaceae, Cystobasidiaceae, Debaryomycetaceae, Dipodascaceae, Enter obacter iaceae, Enter ococcaceae, Erwiniaceae, Gymnoascaceae, Elelotiaceae, Elypocreaceae, Lactobacillaceae, Meruliaceae, Microascaceae, Microbacteriaceae, Mrakiaceae, Nitr obacteraceae, Ophiocordycipitaceae, Oscillospiraceae, Oxalobacter aceae, Paenibacillaceae, Pichiaceae, Propionibacteriaceae, Pseudomonadaceae, Rhizobiaceae, Rhizopodaceae, Saccharomycetaceae, WO 2024/168313 PCT/US2024/015273 Sphingomonadaceae, Sirobasidiaceae, Streptococcaceae, Streptomycetaceae, Thermoascaceae, Xanthobacteraceae, Xanthomonadaceae, and/or Xylariaceae. [00102]In some embodiments, the microbial biomass comprises one or more organism comprising at least one polynucleotide sequence that is at least 95%, 86%, 97%, 98%, 98.5%, 99%, 99.5%, 99.9%, 100% identical to one or more of SEQ ID NOs. 5-57, 114-122, or combinations thereof. [00103]In some embodiments, the microbial biomass comprises one or more organism comprising at least one or more genes encoding a protein whose amino acid sequence is selected from the group consisting of SEQ ID NOs. 58-113, or combinations thereof. [00104]In some embodiments, the microbial biomass comprises a plurality of microorganisms. [00105]In some embodiments, the formulation is a dry solid comprising living microbial biomass present in a concentration of at least 1.0E+01 CFU/g, at least 1.0E+02 CFU/g, at least 1.0E+03 CFU/g, at least 1.0E+05 CFU/g, at least 1.0E+08 CFU/g, at least 1.0E+10 CFU/g, at least 1.0E+11 CFU/g, or at least 1.0E+12 CFU/g. [00106]In some embodiments, the formulation is a dry solid comprising living microbial biomass present in a concentration between about 1.0E+06 CFU/g and 5.0E+11 CFU/g. [00107]In some embodiments, the formulation is a liquid comprising living microbial biomass present in a concentration of at least 1.0E+01 CFU/g, at least 1.0E+02 CFU/g, at least 1.0E+CFU/mL, at least 1.0E+05 CFU/mL, at least 1.0E+08 CFU/mL, at least 1.0E+10 CFU/mL, at least LOE+11 CFU/g, or at least LOE+12 CFU/g. [00108]In some embodiments, the formulation is a liquid comprising living microbial biomass present in a concentration between about 1.0E+06 CFU/g and 5.0E+11 CFU/mL. [00109]In some embodiments, the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+02, at least 1.0E+03, at least 1.0E+04, at least 1.0E+05 CFU/g in the formulation for at least 30, at least 60, at least 90 days, at least 120 days, at least 150 days, at least 200 days, or at least 250 days. [00110]In some embodiments, the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+02, at least 1.0E+03, at least 1.0E+04, at least 1.0E+05 CFU/g in the formulation at least about 4 degrees C, at least about 22 degrees C, or at least about 35 degrees C.

WO 2024/168313 PCT/US2024/015273 id="p-111" id="p-111"

[00111]In some embodiments, the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+01 CFU/seed, at least 1.0E+02 CFU/seed, at least 1.0E+03 CFU/seed, at least 1.0E+04 CFU/seed, at least 1.0E+05 CFU/seed, at least 1.0E+06 CFU/seed, at least 1.0E+07 CFU/seed, at least 1.0E+08 CFU/seed in the formulation applied to a plant seed for at least 30, at least 60, at least 90 days, at least 120 days, at least 1days, at least 200 days, or at least 250 days. [00112]In some embodiments, the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+01 CFU/seed, at least 1.0E+02 CFU/seed, at least 1.0E+03 CFU/seed, at least 1.0E+04 CFU/seed, or at least 1.0E+05 CFU/seed in the formulation applied to a plant seed when stored at least about 4 degrees C, at least about degrees C, or at least about 35 degrees C. [00113]In some embodiments, the formulation comprises living microbial biomass capable of maintaining a viable concentration in the formulation when stored at between about 20 degrees Celsius and 33 degrees Celsius and about between 10% and 50% relative humidity with less than log loss of CFU over 30 or more days. [00114]In some embodiments, the formulation is applied to a plant element. [00115]In some embodiments, the formulation is a feedstock for spray drying or lyophilization. [00116] In some embodiments, the formulation is a feedstock for an encapsulation technique. [00117]In some embodiments, the formulation comprises 3% to 60% total solids. In some embodiments, the formulation comprises 20% to 40% total solids. [00118]In some embodiments, the formulation is a liquid. [00119]In some embodiments, the formulation is a solid. [00120]In another aspect, the disclosure provides a formulation comprising: (a) at least one antioxidant present at a concentration of between 3.5-8.4% by dry weight of the formulation; (b) at least one pH modifier; (c) at least one saccharide present at a concentration of between 4.3- 42% by dry weight of the formulation; and (d) at least one protein hydrolysate present at a concentration of between 4-19.1% by dry weight of the formulation. [00121]In some embodiments, the at least one antioxidant comprises ascorbic acid or creatine. In some embodiments, the at least one amino acid comprises cysteine or glutathione. In some embodiments, the at least one pH modifier comprises sodium bicarbonate, or sodium hydroxide. In some embodiments, the at least one saccharide comprises maltodextrin, sucrose, lactose, WO 2024/168313 PCT/US2024/015273 trehalose, or microcrystalline cellulose. In some embodiments, the at least one protein hydrolysate comprises peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein. [00122]In some embodiments, the formulation further comprises at least one inert solid present at a concentration of between 10-32% by dry weight of the formulation. In some embodiments, the at least one inert solid comprises kaolin clay, magnesium stearate, or microcrystalline cellulose. [00123]In some embodiments, the at least one saccharide comprises maltodextrin. In some embodiments, the at least one saccharide comprises maltodextrin and trehalose. [00124]In some embodiments, the maltodextrin has a dextrose equivalent (DE) value between and 20. [00125]In some embodiments, the formulation further comprises one or more of a sugar alcohol, an amino acid, yeast extract, and/or whey powder. [00126]In some embodiments, the formulation comprises at least one amino acid present at a concentration of between 2.1-7% by dry weight of the formulation. In some embodiments, the at least one amino acid comprises cysteine or glutathione. [00127]In another aspect, the disclosure provides a formulation comprising; (a) between about 30-45% by dry weight of maltodextrin; (b) between about 18-19.1% by dry weight of peptone; (c) between about 4.4-5.4% by dry weight of ascorbic acid; and (d) between about 1.6-2.5% by dry weight of sodium bicarbonate. [00128]In some embodiments, the formulation further comprises between about 29-32% by dry weight of kaolin clay. [00129]In some embodiments, maltodextrin has a dextrose equivalent (DE) of between about and 14. [00130]In another aspect, the disclosure provides a formulation comprising; (a) between about 30-45% by dry weight of maltodextrin; (b) between about 14-19.1% by dry weight of peptone; (c) between about 3.5-4.5% by dry weight of casamino acids; (d) between about 4.5-7% by dry weight of sorbitol; (e) between about 3.2-5.4% by dry weight of ascorbic acid; (f) between about 4.2-7.2% by dry weight of trehalose; and (g) between about 1.6-2.5% by dry weight of sodium bicarbonate.

WO 2024/168313 PCT/US2024/015273 id="p-131" id="p-131"

[00131]In some embodiments, wherein the formulation further comprises between about 21.2- 32% by dry weight of kaolin clay. In some embodiments, maltodextrin has a dextrose equivalent (DE) of at least 14. [00132]In another aspect, the disclosure provides a formulation comprising: (a) between about 30-45% by dry weight of maltodextrin; (b) between about 16-19.1% by dry weight of peptone; (c) between about 4.4-6% by dry weight of ascorbic acid; (d) between about 4-6% by dry weight of trehalose; and (e) between about 1.6-2.5% by dry weight of sodium bicarbonate. [00133]In some embodiments, the formulation further comprises between about 29-32% by dry weight of kaolin clay. In some embodiments, maltodextrin has a dextrose equivalent (DE) of between about 3 and 6. [00134]In another aspect, the disclosure provides a formulation comprising; (a) between about 30-45% by dry weight of maltodextrin; (b) between about 13.6-19.1% by dry weight of peptone; (c) between about 4.4-10% by dry weight of ascorbic acid; (d) between about 2.1-7.9% by dry weight of sodium bicarbonate; and (e) between about 2.1-5.1% by dry weight of cysteine. [00135]In some embodiments, the formulation further comprises between about 21.2-32% by dry weight of kaolin clay. In some embodiments, maltodextrin has a dextrose equivalent (DE) of between about 6 and 14. [00136]In some embodiments, ascorbic acid is L-ascorbic acid. [00137]In some embodiments, the formulation further comprises one or more an oil, a wax, an antioxidant, a pH modifier, a surfactant, an adherent, a bulking agent, a solid diluent, a tackifier, a microbial stabilizer, an antimicrobial, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, a polymer, a protein hydrolysate, a plant extract, rheology modifier, a dispersant, an emulsifier, a colorant, a pH buffer, and/or an anticoagulant. [00138]In some embodiments, the formulation is applied to a plant element. [00139]In another aspect, the disclosure provides a method for preparing a synthetic composition. In some embodiments, the method comprises heterologously disposing a plurality of plant elements with the formulation of any one of the above aspects or embodiments (e.g., a formulation disclosed herein).

WO 2024/168313 PCT/US2024/015273 id="p-140" id="p-140"

[00140]In some embodiments, the formulation comprises microbial biomass comprising at least one microorganism, wherein at least one microorganism within the synthetic composition exhibits increased viability compared to a reference microorganism. [00141]In some embodiments, the at least one microorganism within the synthetic composition retains viability for at least 50 days, at least 100 days, at least 150 days, at least 200 days, at least 250 days, at least 300 days, at least 400 days, or at least 500 days when stored at 4 degrees Celsius. [00142]In some embodiments, the at least one microorganism within the synthetic composition retains viability for at least 50 days, at least 100 days, at least 150 days, at least 200 days, at least 250 days, at least 300 days, at least 400 days, or at least 500 days when stored at 22 degrees Celsius. [00143]In some embodiments, the time to 1 log loss in CPU of the at least one microorganism in synthetic composition is at greater than or equal to 168 days, greater than or equal to 150 days, greater than or equal to 125 days, greater than or equal to 100 days, greater than or equal to days, greater than or equal to 50 days, greater than or equal to 20 days, at 4 degrees Celsius. [00144]In some embodiments, the time to 2 log loss in CPU of the at least one microorganism on a seed is at least 3 days, at least 5 days, at least 10 days, at least 20 days, at least 30 days, at least 60 days, at least 100 days, at least 200 days, at 22 degrees Celsius. [00145]In another aspect, the disclosure provides a method of improving plant health. In some embodiments, the method comprises heterologously disposing an effective amount of one or more microorganisms to a plant element to increase a trait of agronomic importance in the plant derived from the treated plant element relative to a plant derived from a reference plant element, wherein the one or more microorganisms are comprised within the formulation of any one of the above aspects or embodiments (e.g., a formulation disclosed herein). [00146]In some embodiments, the treated plant exhibits an improvement in tolerance to a stress condition relative to a plant derived from a reference plant element. [00147]In some embodiments, the stress condition is an abiotic stress selected from the group consisting of: drought stress, salt stress, metal stress, heat stress, cold stress, low nutrient stress, and excess water stress, and combinations thereof [00148]In some embodiments, the stress condition is a biotic stress selected from the group consisting of: insect infestation, nematode infestation, complex infection, fungal infection, WO 2024/168313 PCT/US2024/015273 bacterial infection, oomycete infection, protozoal infection, viral infection, herbivore grazing, and combinations thereof [00149]In some embodiments, an improvement in stress tolerance is evaluated by improvement of one or more other traits of agronomic importance when compared with a reference plant, reference plant element, or reference population. [00150]In some embodiments, an improvement in biotic stress tolerance is evaluated by one or more of: decreased pathogen load of tissues, decreased area of chlorotic tissue, decreased necrosis, improved growth, increased survival, increased biomass, increased shoot height, increased root length, or combinations thereof relative to a reference plant derived from a reference plant element. [00151]In another aspect, the disclosure provides a method for producing a spray dried formulation. In some embodiments, the method comprises (a) preparing a feedstock comprising microbial biomass; one or more antioxidant in a ratio of about 0.07 to 14 by weight relative to the microbial biomass; one or more pH modifier in a ratio of about 0.03 to 7 by weight relative to the microbial biomass; one or more saccharide in a ratio of about 0.07 to 62 by weight relative to the microbial biomass; and one or more protein hydrolysate in a ratio of about 0.07 to by weight relative to the microbial biomass, the microbial biomass have a first concentration; (b) spray drying the feedstock, wherein spray drying comprises introducing the feedstock into the spray dryer using an inlet temperature of between about 75 degrees Celsius and 140 degrees Celsius and an outlet temperature of between about 45 degrees Celsius and 65 degrees Celsius, to generate an emitted spray dried formulation having a second concentration. [00152]In some embodiments, the feedstock comprises one or more antioxidant in a ratio of at least about 0.7 by weight relative to the microbial biomass; one or more pH modifier in a ratio of at least about 0.3 by weight relative to the microbial biomass; one or more saccharide in a ratio of at least about 1 by weight relative to the microbial biomass; and one or more protein hydrolysate in a ratio of at least about 0.7 by weight. [00153]In some embodiments, (a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; (b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; (c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass; and (d) the one or more protein WO 2024/168313 PCT/US2024/015273 hydrolysate comprises peptone in a ratio of about 1.45 to 29 by weight relative to the microbial biomass. [00154]In some embodiments, (a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; (b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; (c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass and trehalose present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass; (d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.2 to 24 by weight relative to the microbial biomass and casamino acids present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and (e) the formulation additionally comprises sorbitol present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass. [00155]In some embodiments, (a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; (b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; (c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass and trehalose present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and (d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass. [00156]In some embodiments, (a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to 14 by weight relative to the microbial biomass; (b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; (c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to 62 by weight relative to the microbial biomass; (d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass; and (e) the formulation additionally comprises cysteine present in a ratio of about 0.6 to 12 by weight relative to the microbial biomass. [00157]In some embodiments, the second concentration is at least 20%, at least 40%, or at least 75% of the first concentration.

WO 2024/168313 PCT/US2024/015273 id="p-158" id="p-158"

[00158]In some embodiments, the second concentration is at least 1.0E+03 CFU/g, at least 1.0E+05 CFU/g, or at least 1.0E+08 CFU/g. [00159]In some embodiments, the at least one microorganism within the spray dried formulation composition retains viability for at least 30 days, 50 days, at least 100 days, at least 150 days, at least 200 days, at least 250 days, at least 300 days, at least 400 days, or at least 5days when stored at 4 degrees Celsius, as measured by a less than one log loss in the second concentration over that time. [00160]In some embodiments, the at least one microorganism within the spray dried formulation composition retains viability for at least 30 days, 50 days, at least 100 days, at least 150 days, at least 200 days, at least 250 days, at least 300 days, at least 400 days, or at least 5days when stored at 22 degrees Celsius, as measured by a less than one log loss in the second concentration over that time.

DETAILED DESCRIPTION id="p-161" id="p-161"

[00161]Terms used in the claims and specification are defined as set forth below unless otherwise specified. [00162]It must be noted that, as used in the specification and the appended claims, the singular forms "a," "an" and "the" include plural referents unless the context clearly dictates otherwise. A range of values is considered to be inclusive of beginning and ending values unless context clearly dictates otherwise. [00163]Many of the microorganisms described herein have a biological activity, a direct or indirect effect upon a living organism or environment. One example of a biological activity is an improvement in plant health. "Plant health" is demonstrated by the improvement of a trait of agronomic importance in a plant or plant element as compared to a reference plant or reference plant element. A trait of agronomic importance includes, but is not limited to, drought tolerance, heat tolerance, cold tolerance, salinity tolerance, metal tolerance, herbicide tolerance, improved water use efficiency, improved nitrogen utilization, improved nitrogen fixation, improved nutrient use efficiency, improved nutrient utilization, biotic stress tolerance, yield improvement, health enhancement, vigor improvement, decreased necrosis, decreased chlorosis, decreased area of necrotic tissue, decreased area of chlorotic tissue, decreased pathogen load of tissues, growth improvement, photosynthetic capability improvement, nutrition enhancement, altered protein WO 2024/168313 PCT/US2024/015273 content, altered oil content, increased biomass, increased shoot height, increased root length, increased shoot biomass, increased root biomass, increased leaf area, increased shoot area, increased root area, improved root architecture, increased seed germination percentage, increased seed germination rate, increased seedling survival, increased survival, photosynthetic efficiency, transpiration rate, seed/fruit number or mass, plant grain or fruit yield, leaf chlorophyll content, photosynthetic rate, wilt recovery, turgor pressure, modulation of a metabolite, production of a volatile organic compound (VOC), modulation of the proteome, increased seed weight, altered seed carbohydrate composition, altered seed oil composition, altered seed protein composition, altered seed nutrient composition, and combinations thereof. The phrase "biotic stress" refers to a growth environment comprising one or more pests or pathogens. Pests can be nematodes and/or insects. In some embodiments, a pest is of an order Lepidoptera, Hemiptera, TylenchidalRhabditida, Dorylaimida, Trichinellida, or Triplonchida. In some embodiments, a pest is of the order Lepidoptera, Hemiptera, TylenchidalRhabditida, Dorylaimida, Heterodera, Meloidogyne, Pratylenchus, Trichinellida, Globodera, Xiphinema, Hoplolaimus, Longidorus, Rotylenchulus, Helicotylenchus, Belonolaimus, Trichodorus, Paratrichodorus, Tylenchorhynchus, Anguillulina, Merlinia, or Triplonchida. Pathogens can be fungal, viral, protist, or bacterial pathogens, for example of vertebrates or plants. In some embodiments, a pathogen is of a genera Pythium, Rhizoctonia, Phytophthora, Fusarium, Alternaria, Stagonospora, Aspergillus, Magnaporthe, Botrytis, Puccinia, Blumeria, Erysiphe, Leveillula, Mycosphaerella, Colletotrichum Macrophomina, Cercospora sojina, Corynespora, Phomopsis, and Cercospora. [00164]Other examples of biological activity include improvement to a condition affecting health or wellbeing, disease or distress, or function of organisms other than plants, including animals (including humans), insect, reptile, amphibian, birds, bacteria, fungi, etc. A biological activity may be an improvement in a measured or perceived quality of a characteristic of a food or manufactured product such as a flavor, smell, resistance to decay, healthfulness, texture, composition, yield, etc. A biological activity may be an improved efficacy or reduced negative effect of a treatment for a medical condition. A biological activity of an organism may be measured by any assay capable of detecting the phenotype of interest.

WO 2024/168313 PCT/US2024/015273 id="p-165" id="p-165"

[00165] ، ‘Biomass" means the total mass or weight (fresh or dry), at a given time (for example, age or stage of development), of an organism, for example, a microorganism. The term may also refer to all the organisms in the community ("community biomass"). [00166]An "increased yield " can refer to any increase in biomass, for example, in plants yield may refer to seed, seed pod or ear, or fruit number per plant; or seed or fruit weight; or seed or fruit size per plant or unit of production area, e.g. acre or hectare. For example, increased yield of seed or fruit biomass may be measured in units of bushels per acre, pounds per acre, tons per acre, or kilos per hectare. An increased yield can also refer to an increased production of a component of, or product derived from, a plant or plant element or of a unit of measure thereof. For example, increased carbohydrate yield of a grain or increased oil yield of a seed. Typically, where yield indicates an increase in a particular component or product derived from a plant, the particular characteristic is designated when referring to increased yield, e.g., increased oil or grain yield or increased protein yield or seed size. [00167]Synthetic compositions and methods of use described herein may improve plant health by providing an improved benefit or tolerance to a plant that is of at least 0.1%, at least 0.5%, at least 1%, at least 2%, at least 3%, between 3% and 5%, at least 5%, between 5% and 10%, at least 10%, between 10% and 15%, at least 15%, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 75%, at least 75%, between 75% and 100%, at least 100%, between 100% and 150%, at least 150%, between 150% and 200%, at least 200%, between 200% and 300%, at least 300% or more, when compared with a reference plant. A "reference plant", "reference plant element", "reference agricultural plant" or "reference seed " means a similarly situated plant or seed of the same species, strain, or cultivar to which a treatment, formulation, composition, or microorganism preparation as described herein is not administered/contacted. A reference plant, therefore, is identical to the treated plant except for the presence of the active ingredient to be tested and can serve as a control for detecting the effects of the treatment conferred to the plant. A plurality of reference plants may be referred to as a "reference population". [00168]In some embodiments, one or more microorganisms and/or one or more compounds produced by one or more microorganisms are heterologously disposed on a plant element in an effective amount to improve plant health. In some embodiments, an improvement of plant health WO 2024/168313 PCT/US2024/015273 is measured by an increase in a trait of agronomic importance, for example root length or yield. In some embodiments, an improvement of subject health is measured by a decrease in a trait of importance, for example necrosis or chlorosis. In some embodiments, improved plant health is demonstrated by an improvement of a trait of agronomic importance or tolerance in a treated plant by at least 0.1%, at least 0.5%, at least 1%, at least 2%, at least 3%, between 3% and 5%, at least 5%, between 5% and 10%, at least 10%, between 10% and 15%, at least 15%, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 75%, at least 75%, between 75% and 100%, at least 100%, between 100% and 150%, at least 150%, between 150% and 200%, at least 200%, between 200% and 300%, at least 300% or more, as compared to a reference plant element not further comprising said microorganism. An "effective amount" of one or more microorganisms is the amount capable of improving trait of agronomic importance or tolerance by at least 0.1%, at least 0.5%, at least 1%, at least 2%, at least 3%, between 3% and 5%, at least 5%, between 5% and 10%, at least 10%, between 10% and 15%, at least 15%, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 75%, at least 75%, between 75% and 100%, at least 100%, between 100% and 150%, at least 150%, between 150% and 200%, at least 200%, between 200% and 300%, at least 300% or more, as compared to a reference plant element not further comprising said microorganism. In some embodiments, an effective amount of treatment comprising a microorganism is at least 10 CPU per unit of plant element, at least 10A2 CPU per unit of plant element, between 10A2 and 10A3 CPU per unit of plant element, at least about 10ACPU per unit of plant element, between 10A3 and 10A4 CPU per unit of plant element, at least about 10A4 CPU per unit of plant element, between 10A4 and 10A5 CPU per unit of plant element, at least about 10A5 CPU, between 10A5 and 10A6 CPU per unit of plant element, at least about 10A6 CPU per unit of plant element, between 10A6 and 10A7 CPU per unit of plant element, at least about 10A7 CPU per unit of plant element, between 10A7 and 10A8 CPU per unit of plant element, or even greater than 10A8 CPU per unit of plant element. A unit of a plant element may be an individual plant element, e.g. an individual seed, or a unit of area surface area of a plant element, e.g. a square inch of leaf tissue, or unit of surface area of a plant element, e.g. a cubic centimeter of root.

WO 2024/168313 PCT/US2024/015273 id="p-169" id="p-169"

[00169]In some embodiments, an effective amount of treatment comprising a microorganism is at least 10 CFU per gram of synthetic composition, at least 10A2 CFU per gram of synthetic composition, between 10A2 and 10A3 CFU per gram of synthetic composition, at least about 10A3 CFU per gram of synthetic composition, between 10A3 and 10A4 CFU per gram of synthetic composition, at least about 10A4 CFU per gram of synthetic composition, between 10A4 and 10A5 CFU per gram of synthetic composition, at least about 10A5 CFU per gram of synthetic composition, between 10A5 and 10A6 CFU per gram of synthetic composition, at least about 10A6 CFU per gram of synthetic composition, between 10A6 and 10A7 CFU per gram of synthetic composition, at least about 10A7 CFU per gram of synthetic composition, between 10A7 and 10A8 CFU per gram of synthetic composition, at least about 10A8 CFU per gram of synthetic composition, between 10A8 and 10A9 CFU per gram of synthetic composition, at least about 10A9 CFU per gram of synthetic composition, between 10A9 and 10A10 CFU per gram of synthetic composition, or even greater than 10A10 CFU per unit of plant element. In some embodiments, a synthetic composition comprises a microorganism heterologously disposed in a treatment formulation at concentration of at least 10A6 CFU per gram. [00170]The methods and compositions of the present invention are broadly applicable to cultivated plants, particularly plants that are cultivated by humans for food, feed, fiber, fuel, and/or industrial purposes. In some embodiments, plants (including seeds and other plant elements) are monocots or dicots. In some embodiments, plants used in the methods and compositions of the present invention include, but are not limited to: agricultural row, agricultural grass plants or other field crops; wheat, rice, barley, buckwheat, beans (for example: soybean, snap, dry), corn (for example: grain, seed, sweet corn, silage, popcorn, high oil), canola, sugar cane, peas (for example: dry, succulent), peanuts, safflower, sunflower, alfalfa hay, forage and cover crops (for example: alfalfa, clover, vetch, and trefoil), berries and small fruits (for example: blackberries, blueberries, currants, elderberries, gooseberries, huckleberries, loganberries, raspberries, strawberries, bananas, and grapes), bulb crops (for example: garlic, leeks, onions, shallots, and ornamental bulbs), citrus fruits (for example: citrus hybrids, grapefruit, kumquat, lines, oranges, and pummelos), cucurbit vegetables (for example: cucumbers, melons, gourds, pumpkins, and squash), flowers (for example: ornamental, horticultural flowers including roses, daisies, tulips, freesias, carnations, heather, lilies, irises, orchids, snapdragons, and ornamental sunflowers), bedding plants, ornamentals, fruiting WO 2024/168313 PCT/US2024/015273 vegetables (for example: eggplant, sweet and hot peppers, tomatillos, and tomatoes), herbs, spices, mints, hydroponic crops (for example: cucumbers, tomatoes, lettuce, herbs, and spices), leafy vegetables and cole crops (for example: arugula, celery, chervil, endive, fennel, lettuce including head and leaf, parsley, radicchio, rhubarb, spinach, Swiss chard, broccoli, Brussels sprouts, cabbage, cauliflower, collards, kale, kohlrabi, and mustard greens), asparagus, legume vegetable and field crops (for example: snap and dry beans, lentils, succulent and dry peas, and peanuts), pome fruit (for example: pears and quince), root crops (for example: beets, sugar beets, red beets, carrots, celeriac, chicory, horseradish, parsnip, radish, rutabaga, salsify, and turnips), deciduous trees (for example: maple and oak), evergreen trees (for example: pine, cedar, hemlock and spruce), small grains (for example: rye, wheat including spring and winter wheat, millet, oats, barley including spring and winter barley, and spelt), stone fruits (for example: apricots, cherries, nectarines, peaches, plums, and prunes), tree nuts (for example: almonds, beech nuts, Brazil nuts, butternuts, cashews, chestnuts, filberts, hickory nuts, macadamia nuts, pecans, pistachios, and walnuts), and tuber crops (for example: potatoes, sweet potatoes, yams, artichoke, cassava, and ginger). In a particular embodiment, the agricultural plant is selected from the group consisting of rice (Oryza sativa and related varieties), soy (Glycine max and related varieties), wheat (Triticum aestivum and related varieties), oats (Avena sativa and related varieties), barley (Hordeum vulgare and related varieties), corn (Zea mays and related varieties), peanuts (Arachis hypogaea and related varieties), canola (Brassica napus, Brassica rapa and related varieties), coffee (Coffea spp.), cocoa (Theobroma cacao), melons, and tomatoes (Solanum lycopsersicum and related varieties). [00171]Plant health may be improved by treatment of a plant or plant element. A "plant element" is intended to generically reference either a whole plant or a plant component, including but not limited to plant tissues, parts, and cell types. A plant element is preferably one of the following: whole plant, seedling, meristematic tissue, ground tissue, vascular tissue, dermal tissue, seed, leaf, root, shoot, stem, flower, fruit, stolon, bulb, tuber, corm, keikis, or bud. [00172]Plant health may be improved by treatment with a composition of the present invention, in particular compositions of the present invention comprising one or more microorganisms. An "endophyte " is an organism capable of living in close association with a plant element, for example, on a plant element (e.g., rhizoplane or phyllosphere) or within a plant element, or on a surface in close physical proximity with a plant element, e.g., the phyllosphere and rhizosphere WO 2024/168313 PCT/US2024/015273 including soil surrounding roots. A "beneficial" endophyte does not cause disease or harm the host plant otherwise. Endophytes can occupy the intracellular or extracellular spaces of plant tissue, including the leaves, stems, flowers, fruits, seeds, or roots. An endophyte can be, for example, a bacterial or fungal organism, and can confer a beneficial property to the host plant such as an increase in yield, biomass, resistance, or fitness. An endophyte can be a fungus or a bacterium. As used herein, the term "microbe" is sometimes used to describe an endophyte. As used herein, the term "microbe" or "microorganism " refers to any species or taxon of microorganism, including, but not limited to, archaea, bacteria, microalgae, fungi (including mold and yeast species), mycoplasmas, microspores, nanobacteria, oomycetes, virus, phage, biological agent, fungal agent, bacterial agent, and protozoa. In some embodiments, a microbe or microorganism is an endophyte, for example a bacterial or fungal endophyte, which is capable of living within a plant. In some embodiments, a microbe or microorganism is alive, inactivated, attenuated, or dead. Microorganisms may include one or more biological agent. A biological agent is any organism or substance of biological origin which has biological activity, e.g. an effect upon a living organism. A biological agent may be a bacterial, fungal, or viral agent and may be living, attenuated, dead, or inactivated (including without limitation by heat inactivation). A biological agent can be one or more extracts and/or metabolites of an organism (animal, plant, insect, bacteria, fungi, etc.) including without limitation extracts and/or metabolites in spent growth media. [00173]The term "isolated " is intended to specifically reference an organism, cell, tissue, polynucleotide, or polypeptide that is removed from its original source and purified from additional components with which it was originally associated. For example, a microorganism may be considered isolated from a seed if it is removed from that seed source and purified so that it is isolated from one or more additional components with which it was originally associated. Similarly, a microorganism may be removed and purified from a plant or plant element so that it is isolated and no longer associated with its source plant or plant element. [00174]As used herein, an isolated strain of a microbe is a strain that has been removed from its natural milieu. "Pure cultures" or "isolated cultures" are cultures in which the organisms present are only of one strain of a particular genus and species. Conversely "mixed cultures," are cultures in which more than one genus and/or species of microorganism are present. As such, the term "isolated " does not necessarily reflect the extent to which the microbe has been purified. A WO 2024/168313 PCT/US2024/015273 "substantially pure culture" of the strain of microbe refers to a culture which contains substantially no other microbes than the desired strain or strains of microbe. In other words, a substantially pure culture of a strain of microbe is substantially free of other contaminants, which can include microbial contaminants. Further, as used herein, a "biologically pure" strain is intended to mean the strain was separated from materials with which it is normally associated in nature. A strain associated with other strains, or with compounds or materials that it is not normally found with in nature, is still defined as "biologically pure." A monoculture of a particular strain is, of course, "biologically pure." As used herein, the term "enriched culture" of an isolated microbial strain refers to a microbial culture that contains more that 50%, 60%, 70%, 80%, 90%, or 95% of the isolated strain. [00175]A "population" of microorganisms, or a "microorganism population", refers to one or more microorganisms that share a common genetic derivation, e.g., one or more propagules of a single microorganism, i.e., microorganisms grown from a single picked colony. In some embodiments, a population refers to microorganisms of identical taxonomy. In some cases, a population of microorganisms refers to one or more microorganisms of the same genus. In some cases, a population of microorganisms refers to one or more microorganisms of the same species or strain. [00176]A "plurality of microorganisms " means two or more types of microorganism entities, e.g., of bacteria or fungi, or combinations thereof. In some embodiments, the two or more types of microorganism entities are two or more individual endophytic organisms, regardless of genetic derivation or taxonomic relationship. In some embodiments, the two or more types of microorganism entities are two or more populations of microorganisms. In other embodiments, the two or more types of microorganism entities are two or more species of microorganisms. In yet other embodiments, the two or more types of microorganism entities are two or more genera of microorganisms. In yet other embodiments, the two or more types of microorganism entities are two or more families of microorganisms. In yet other embodiments, the two or more types of microorganism entities are two or more orders of microorganisms. In yet other embodiments, the two or more types of microorganism entities are two or more classes of microorganisms. In yet other embodiments, the two or more types of microorganism entities are two or more phyla of microorganisms. In some embodiments, a plurality refers to three or more microorganisms, either distinct individual organisms or distinct members of different genetic derivation or taxa. In WO 2024/168313 PCT/US2024/015273 some embodiments, a plurality refers to four or more either distinct individual microorganisms or distinct members of different genetic derivation or taxa. In some embodiments, a plurality refers to five or more, ten or more, or an even greater number of either distinct individual microorganisms or distinct members of different genetic derivation or taxa. In some embodiments, the term "consortium" or "consortia" may be used as a collective noun synonymous with "plurality", when describing more than one population, species, genus, family, order, class, or phylum of microorganisms. [00177]In some embodiments, a treatment may comprise a modified microbe or plant or plant element. A microbe or plant or plant element is "modified " when it comprises an artificially introduced genetic or epigenetic modification. In some embodiments, the modification is introduced by a genome engineering or genome editing technology. In some embodiments, genome engineering or editing utilizes non-homologous end joining (NHEJ), homology directed repair (HDR), or combinations thereof. In some embodiments, genome engineering or genome editing is carried out with a Class I or Class II clustered regulatory interspaced short palindromic repeats (CRISPR) system. In some embodiments, the CRISPR system is CRISPR/Cas9. In some embodiments, the CRISPR system is CRISPR/Cpfl. In some embodiments, the modification is introduced by a targeted nuclease. In some embodiments, targeted nucleases include, but are not limited to, transcription activator-like effector nuclease (TALEN), zinc finger nuclease (ZNF), Cas9, Cas9 variants, Cas9 homologs, Cpfl, Cpfl variants, Cpfl homologs, and combinations thereof. In some embodiments, the modification is an epigenetic modification. In some embodiments, the modification is introduced by treatment with a DNA methyltransferase inhibitor such as 5-azacytidine, or a histone deacetylase inhibitor such as 2-amino-7-methoxy- 3H-phenoxazin-3-one. In some embodiments, the modification is introduced via tissue culture. In some embodiments, a modified microbe or plant or plant element comprises a transgene. [00178]As used herein, the term "bacterium" or "bacteria" refers in general to any prokaryotic organism and may reference an organism from either Kingdom Eubacteria (Bacteria), Kingdom Archaebacteria (Archaea), or both. In some cases, bacterial genera have been reassigned due to various reasons (such as, but not limited to, the evolving field of whole genome sequencing), and it is understood that such nomenclature reassignments are within the scope of any claimed genus. [00179]As used herein, the term "fungus " or "fungi " refers in general to any organism from Kingdom Fungi. Historical taxonomic classification of fungi has been according to WO 2024/168313 PCT/US2024/015273 morphological presentation. Beginning in the mid-1800 ’s, it was recognized that some fungi have a pleomorphic life cycle, and that different nomenclature designations were being used for different forms of the same fungus. With the development of genomic sequencing, it became evident that taxonomic classification based on molecular phylogenetics did not align with morphological-based nomenclature (Shenoy BD, Jeewon R, Hyde KD. Impact of DNA sequence-data on the taxonomy of anamorphic fungi. Fungal Diversity 26(10) 1-54. 2007). Systematics experts have not aligned on common nomenclature for all fungi, nor are all existing databases and information resources inclusive of updated taxonomies. As such, many fungi provided herein may be described by their anamorph form, but it is understood that based on identical genomic sequencing, any pleomorphic state of that fungus may be considered to be the same organism. In some cases, fungal genera have been reassigned due to various reasons, and it is understood that such nomenclature reassignments are within the scope of any claimed genus. [00180]The degree of relatedness between microbes may be inferred from the sequence similarity of one or more homologous polynucleotide sequences of the microbes. In some embodiments, the one or more homologous polynucleotide sequences are marker genes. As used herein, the term "marker gene " refers to a conserved genomic region comprising sequence variation among related organisms. Examples of marker genes that may be used for the present invention, include but are not limited to: 16S ribosomal RNA gene ("16S"), internal transcribed spacer ("ITS"); fusA gene; largest subunit of RNA polymerase II ("RPB1"); second largest subunit of RNA polymerase II ("RPB2"); beta-tubulin or tubulin ("BTUB2" or "TUB2"); phosphoglycerate kinase ("PGK"); actin ("ACT"); long subunit rRNA gene ("LSU"); small subunit rRNA gene ("SSU"), 60S ribosomal protein L 10 ("60S L10 L1"), atpD, Calmodulin ("CMD"), GDP gene ("GPD1_2"), etc. [00181]The terms "sequence similarity", "identity ", "percent identity ", "percent sequence identity" or "identical " in the context of polynucleotide sequences refer to the nucleotides in the two sequences that are the same when aligned for maximum correspondence. There are different algorithms known in the art that can be used to measure nucleotide sequence identity. Nucleotide sequence identity can be measured by a local or global alignment, preferably implementing an optimal local or optimal global alignment algorithm. For example, a global alignment may be generated using an implementation of the Needleman-Wunsch algorithm (Needleman, SB. & Wunsch, CD. (1970) Journal of Molecular Biology. 48(3):443-53). For example, a local WO 2024/168313 PCT/US2024/015273 alignment may be generated using an implementation of the Smith-Waterman algorithm (Smith T.F & Waterman, M.S. (1981) Journal of Molecular Biology. 147(1): 195-197). Optimal global alignments using the Needleman-Wunsch algorithm and optimal local alignments using the Smith-Waterman algorithm are implemented in USEARCH, for example USEARCH version v8.1.1756_i860sx32. [00182]A gap is a region of an alignment wherein a sequence does not align to a position in the other sequence of the alignment. A terminal gap is a region beginning at the end of a sequence in an alignment wherein the nucleotide in the terminal position of that sequence does not correspond to a nucleotide position in the other sequence of the alignment and extending for all contiguous positions in that sequence wherein the nucleotides of that sequence do not correspond to a nucleotide position in the other sequence of the alignment. An internal gap is a gap in an alignment which is flanked on the 3’ and 5’ end by positions wherein the aligned sequences are identical. In global alignments, terminal gaps are discarded before identity is calculated. For both local and global alignments, internal gaps are counted as differences. [00183]In some embodiments, the nucleic acid sequence to be aligned is a complete gene. In some embodiments, the nucleic acid sequence to be aligned is a gene fragment. In some embodiments, the nucleic acid sequence to be aligned is an intergenic sequence. In a preferred embodiment, inference of homology from a sequence alignment is made where the region of alignment is at least 85% of the length of the query sequence. [00184]The term "substantial homology " or "substantial similarity," when referring to a polynucleotide sequence or fragment thereof, indicates that, when optimally aligned with appropriate nucleotide insertions or deletions with another polynucleotide sequence (or its complementary strand), there is nucleotide sequence identity in at least about 76%, 80%, 85%, or at least about 90%, or at least about 95%, 96%, at least 97%, 98%, 99% or 100% of the positions of the alignment, wherein the region of alignment is at least about 50%, 60%, 70%, 75%, 85%, or at least about 90%, or at least about 95%, 96%, 97%, 98%, 99% or 100% of the length of the query sequence. In a preferred embodiment, the region of alignment contains at least 1positions inclusive of any internal gaps. In some embodiments, the region of alignment comprises at least 100 nucleotides of the query sequence. In some embodiments, the region of alignment comprises at least 200 nucleotides of the query sequence. In some embodiments, the region of alignment comprises at least 300 nucleotides of the query sequence. In some WO 2024/168313 PCT/US2024/015273 embodiments, the region of alignment comprises at least 400 nucleotides of the query sequence. In some embodiments, the region of alignment comprises at least 500 nucleotides of the query sequence. In some embodiments, the terminal nucleotides are trimmed from one or both ends of the sequence prior to alignment. In some embodiments, at least the terminal 10, 15, 20, 25, 30, between 20-30, 35, 40, 45, 50, between 25-50 nucleotides are trimmed from the sequence prior to alignment.Synthetic compositions for improving plant health [00185]In some embodiments, a synthetic composition comprises one or more microorganisms capable of improving plant health. A "synthetic composition" comprises one or more microorganisms combined by human endeavor with a heterologously disposed plant element or a formulation, said combination which is not found in nature. In some embodiments, a synthetic composition comprises one or more plant elements or formulation components combined by human endeavor with an isolated, purified microorganism composition. In some embodiments, synthetic composition refers to a plurality of microorganisms in a treatment formulation comprising additional components with which said microorganisms are not found in nature. A microorganism is "heterologously disposed " when mechanically or manually applied, artificially inoculated or disposed onto or into a plant element, seedling, plant or onto or into a plant growth medium or onto or into a treatment formulation so that the microorganism exists on or in the plant element, seedling, plant, plant growth medium, or formulation in a manner not found in nature prior to the application of the treatment, e.g., said combination which is not found in nature in that plant variety, at that time in development, in that tissue, in that abundance, or in that growth condition (for example, drought, flood, cold, nutrient deficiency, etc.). [00186]A "formulation" refers to one or more compositions that facilitate the stability, storage, and/or application of one or more microorganisms. In some embodiments, a formulation refers to the one or more excipients. A formulation may comprise one or more excipients in liquid or dry format. For example, a formulation may comprise a plurality of excipients in a dehydrated state which may be reconstituted with an aqueous solution. [00187]Various formulations are contemplated; several non-limiting examples are provided here. An example formulation referred to herein as comprises, by % of non-microorganism solid contents: 34.9-41.7% maltodextrin, 4-8.3% ascorbic acid, 2.2-5.9% sodium bicarbonate. In some embodiments, the formulation additionally comprises 4% casamino acids, 4.5-5.5% sorbitol, 2- WO 2024/168313 PCT/US2024/015273 3% cysteine, 2-3% glutathione, 12-14% whey, and 25-28% kaolin clay. In some embodiments, the formulation additionally comprises 18.5-19.5% peptone, and 30-35% kaolin clay. In some embodiments, the formulation additionally comprises 15-17.5% peptone, 6-9% cysteine, and 25- 30% kaolin clay. [00188]An example formulation referred to herein as comprises, by % of non-microorganism solid contents: 14-19.5% peptone, 4-8.5% ascorbic acid, 2.2-4.5% sodium bicarbonate, and 27- 33% kaolin clay. In some embodiments, the formulation additionally comprises 36-42% maltodextrin. In some embodiments, the formulation additionally comprises 35-37% maltodextrin, 4-5% casamino acids, 5-7% sorbitol, and 5-7% trehalose. In some embodiments, the formulation additionally comprises 35-37% maltodextrin, and 5-7% cysteine. [00189]In some embodiments, a synthetic composition comprises a microorganism heterologously disposed to formulation. As used herein the combination of a formulation and heterologously disposed microorganism is referred to as a "feedstock ". Optionally, a feedstock may be subjected to a drying process such as spray drying or lyophilization. [00190]The dry powder resulting from drying a feedstock is sometimes referred to as a manufacture use product ("MUP"). MUP is a stable composition that may be further processed into one or multiple products, for example, an end use product such as a pharmaceutical composition (for example, a probiotics), a food product, a seed treatment, a fertilizer composition, etc. A ready-to-use composition comprising MUP is sometimes referred to as "RTU formulation", "RTU composition" or simply as "RTU". In some embodiments a synthetic composition is a RTU composition. Unless otherwise stated, a RTU composition comprises MUP, talc, and 1% mineral oil. "Probiotics" as used herein refer to any living microorganism capable of conveying a benefit to a host (for example, a human or other mammalian host) when consumed in adequate quantities by the host. [00191]In some embodiments, synthetic compositions may comprise any one or more agents such as: an oil, a wax, an antioxidant, a pH modifier, a surfactant, an adherent, a bulking agent, a solid diluent, a tackifier, a microbial stabilizer, an antimicrobial, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, a polymer, a protein hydrolysate, a plant extract, rheology modifier, a dispersant, an emulsifier, a colorant, a pH buffer, an WO 2024/168313 PCT/US2024/015273 anticoagulant, etc. As used herein as a noun, a "treatment" may comprise one or more microorganisms. [00192]In some embodiments, a synthetic composition may comprise one or more polymeric beads comprising one or more microorganisms. In some embodiments, a treatment formulation may consist of one or more polymeric beads comprising one or more microorganisms. A polymeric bead may contain a biodegradable polymer such as alginate, agarose, agar, gelatin, polyacrylamide, chitosan, and polyvinyl alcohol. In some embodiments, the polymeric beads are less than 500 /zm in diameter at their widest point. In some embodiments, the polymeric beads ’ average diameter at their widest point is between 500 /zm and 250 /zm, between 249 /zm and 1/zm, 100 /zm or less, between 100 /zm and 50 /zm, or 50 /zm or less. [00193]In some embodiments, an "agriculturally compatible carrier" can be used to formulate an agricultural formulation or other composition that includes a purified microorganism preparation. As used herein an "agriculturally compatible carrier" refers to any material, other than water, that can be added to a plant element without causing or having an adverse effect on the plant element (e.g., reducing seed germination) or the plant that grows from the plant element, or the like. [00194]In some embodiments, the formulation can include a tackifier or adherent. Such agents are useful for combining the bacterial population of the invention with carriers that can contain other compounds (e.g., control agents that are not biologic), to yield a coating composition. Such compositions help create coatings around the plant or seed to maintain contact between the microbe and other agents with the plant or plant part. In some embodiments, adherents are selected from the group consisting of: alginate, gums, starches, lecithins, formononetin, polyvinyl alcohol, alkali formononetinate, hesperetin, polyvinyl acetate, cephalins, Gum Arabic, Xanthan Gum, Mineral Oil, Polyethylene Glycol (PEG), Polyvinyl pyrrolidone (PVP), Arabino- galactan, Methyl Cellulose, PEG 400, Chitosan, Polyacrylamide, Polyacrylate, Polyacrylonitrile, Glycerol, Triethylene glycol, Vinyl Acetate, Gellan Gum, Polystyrene, Polyvinyl, Carboxymethyl cellulose, Gum Ghatti, and polyoxyethylene-polyoxybutylene block copolymers. [00195]The formulation can also contain a surfactant. Non-limiting examples of surfactants include nitrogen-surfactant blends such as Prefer 28 (Cenex), Surf-N(US), Inhance (Brandt), P- (Wilfarm) and Patrol (Helena); esterified seed oils include Sun-It II (AmCy), MSO (UAP), Scoil (Agsco), Hasten (Wilfarm) and Mes-100 (Drexel); and organo-silicone surfactants include WO 2024/168313 PCT/US2024/015273 SilwetL77 (UAP), Silikin (Terra), Dyne-Amie (Helena), Kinetic (Helena), Sylgard 309 (Wilbur- Ellis), whey (e.g. milk derived, by product of cheese making), and Century (Precision). In one embodiment, the surfactant is present at a concentration of between 0.01% v/v to 10% v/v. In another embodiment, the surfactant is present at a concentration of between 0.1% v/v to 1% v/v. [00196]In certain cases, the formulation includes a microbial stabilizer. Such an agent can include a desiccant. As used herein, a "desiccant " can include any compound or mixture of compounds that can be classified as a desiccant regardless of whether the compound or compounds are used in such concentrations that they in fact have a desiccating effect on the liquid inoculant. Such desiccants are ideally compatible with the bacterial population used and should promote the ability of the microbial population to survive application on the seeds and to survive desiccation. Examples of suitable desiccants include one or more of trehalose, sucrose, glycerol, and Methylene glycol. Other suitable desiccants include, but are not limited to, non- reducing sugars and sugar alcohols (e.g., mannitol or sorbitol). The amount of desiccant introduced into the formulation can range from about 5% to about 50% by weight/volume, for example, between about 10% to about 40%, between about 15% and about 35%, or between about 20% and about 30%. [00197]In some embodiments the formulation includes, for example, solid carriers such as talc, fullers earth, bentonite, kaolin clay, pyrophyllite, bentonite, montmorillonite, diatomaceous earth, acid white soil, vermiculite, and pearlite, and inorganic salts such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, ammonium chloride, and calcium carbonate. Also, organic fine powders such as wheat flour, wheat bran, and rice bran may be used. The liquid carriers include vegetable oils such as soybean oil and cottonseed oil, glycerol, ethylene glycol, polyethylene glycol, propylene glycol, polypropylene glycol, etc. [00198]In some embodiments, the abundance of a microorganism can be estimated by methods well known in the art including, but not limited to, qPCR, community sequencing, flow cytometry, and/or counting colony-forming units. As used herein, a "colony-forming unit" ("CFU") is used as a measure of viable microorganisms (such as bacteria or fungi or the like) in a sample. A CFU is an individual viable cell capable of forming on a solid medium a visible colony whose individual cells are derived by cell division from one parental cell. As used herein, a "plaque forming unit" ("PFU") is used as a measure of viable microorganisms (such as phage) in a sample. PFU may be calculated by serial dilution of a sample containing phage suspension WO 2024/168313 PCT/US2024/015273 with host microorganism sensitive to the phage (e.g. a bacteria). Plating serial dilutions of the suspension results in visible clear regions in the culture of the microorganism sensitive to the phage. These clear regions (plaques) are counted and used to calculate the number of plaque forming units (phage) in the original suspension. It is contemplated herein that PFU may be used interchangeably with CFU as a quantification method or descriptor of a microorganism ’s abundance. It is also contemplated that the skilled artisan would readily appreciate the applicability of either measure or alternate methods of quantification based on a number of factors including the type of microorganism to be quantified. [00199]In some embodiments, the synthetic composition of the present invention comprises one or more of the following: antimicrobial, fungicide, nematicide, bactericide, insecticide, or herbicide. [00200]In some embodiments, the time to 1 log loss in CFU of a microorganism in formulation is at greater than or equal to 168 days, greater than or equal to 150 days, greater than or equal to 125 days, greater than or equal to 100 days, greater than or equal to 75 days, greater than or equal to 50 days, greater than or equal to 20 days, at 4 degrees Celsius. In some embodiments, the time to 1 log loss in CFU of a microorganism in formulation is at least 140 days, at least days, at least 60 days, at least 50 days, at least 30 days, at least 20 days, at 22 degrees Celsius. In some embodiments, the time to 2 log loss in CFU of a microorganism on a seed is at least 3 days, at least 5 days, at least 10 days, at least 20 days, at least 21 days, at least 22 days, at least days, at least 24 days, at least 25 days, at 22 degrees Celsius. [00201]Survival through a drying process is determined by comparing the concentration of biologically active ingredients in the dried powder to the concentration of biologically active ingredients within the feedstock and calculating a precent survival by determining the percentage of biologically active ingredients within the feedstock detectable in the spray dried powder. In some embodiments, a microorganism in a formulation described herein has a percent survival through a drying process of at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%. In some embodiments, a microorganism in a formulation described herein is, after spraying, present in the spray-dried intermediate in a concentration of at least 1.0E+02 CFU/g, at least 1.0E+03 CFU/g, at least 1.0E+04 CFU/g, at least 1.0E+05 CFU/g, at least 1.0E+06 CFU/g, at least 1.0E+07 CFU/g, at least 1.0E+08 CFU/g, WO 2024/168313 PCT/US2024/015273 at least 1.0E+09 CFU/g, at least 1 OE+10 CFU/g, at least 1 .OE+l1 CFU/g, or at least 1 .OE+l CFU/g. [00202]In some embodiments, a treatment is applied mechanically or manually or artificially inoculated to a plant element in a seed treatment, root wash, seedling soak, foliar application, floral application, soil inoculum, in-furrow application, sidedress application, soil pre-treatment, wound inoculation, drip tape irrigation, vector-mediation via a pollinator, injection, osmopriming, hydroponics, aquaponics, aeroponics, and combinations thereof. Application to the plant may be achieved, for example, as a powder for surface deposition onto plant leaves, as a spray to the whole plant or selected plant element, as part of a drip to the soil or the roots, or as a coating onto the plant element prior to or after planting. Such examples are meant to be illustrative and not limiting to the scope of the invention. [00203]In some embodiments of any of the synthetic compositions described herein, the synthetic compositions comprise at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15 or 20 or more microorganisms. In some embodiments, the one or more microorganisms comprise at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15 or 20 or more microorganisms. In some embodiments, the one or more microorganisms are distinct individual organisms or distinct members of different genetic derivation or taxa. [00204]In some embodiments, the synthetic composition is contained within packaging. The packaging can be constructed out of a number of materials suitable for storing a solid (e.g., powder) seed treatment. The packaging may be comprised of a metallized polyester and linear low density polyethylene bag. In some embodiments, the packaging comprises a moisture barrier, reduced gas exchange (for example, oxygen transmission), block (partially or fully) UV and light transmission, are impact resistant, and/or tear resistant. In some embodiments, the packaging comprises at least one exterior surface between 0.025-10 mm in thickness. In some embodiments, the packaging comprises an exterior surface having an average thickness of between 0.025-10 mm. In some embodiments, the packaging comprises an exterior surface having a nearly uniform thickness (e.g. variation in thickness of plus or minus 5 mm or less, variation in thickness of plus or minus 1 mm or less, variation in thickness of plus or minus 0.mm or less, variation in thickness of plus or minus 0.05 mm or less, variation in thickness of plus or minus 0.5 mm or less, variation in thickness of plus or minus 0.05 mm or less, variation in thickness of plus or minus 0.005 mm or less, variation in thickness of plus or minus 0.001 mm or WO 2024/168313 PCT/US2024/015273 less). In some embodiments, the packaging comprises an exterior surface having a nearly uniform thickness except for one or more support regions comprising thicker or more rigid material (where the material of the support region may be the same or different from the material comprising the remainder of the walls). In some embodiments, the packaging comprises an exterior surface having a nearly uniform thickness except for one or more regions having one or more significantly thinner region, for example engineered to break when force is applied. In some embodiments, the packaging comprises one or more polyesters, polyethylene, polystyrene, polyamides (nylon), polyacrylonitrile butadiene (ABS), polylactic acid, aluminum (e.g., foils or sheet), stainless steel, silicone, polylactic acid (PLA), bio-composite (for example, bio- composites comprising polylactic acid and microcrystalline cellulose, polylactic acid and cellulose nanocrystal, gelatin, etc.), and combinations thereof. In some embodiments, the packaging comprises one or more layers, for example an adhesive laminated material having high oxygen and moisture barrier properties. Examples of the packaging comprising multiple layers include metallized polyester and linear low-density polyethylene, polyester, aluminum foil, and linear low-density polyethylene. In some embodiments, the packaging acts as a moisture barrier having a moisture vapor transmission rate (MVTR) of 0.2 g per 100 sq. inches per 24 hours, or lower. In some embodiments, the packaging is constructed from a material having a moisture vapor transmission rate (MVTR) 0.2 g per 100 sq. inches per 24 hours, or lower. In some embodiments, the packaging acts as a moisture barrier having a moisture vapor transmission rate (MVTR) of 0.02 g per 100 sq. inches per 24 hours, or lower. In some embodiments, the packaging is constructed from a material having a moisture vapor transmission rate (MVTR) 0.02 g per 100 sq. inches per 24 hours, or lower. In some embodiments, the packaging has a moisture vapor transmission rate (MVTR) of between 0.002 g per 100 sq. inches per 24 hours and 0.2 g per 100 sq. inches per 24 hours. In some embodiments, the packaging is constructed from a material having a moisture vapor transmission rate (MVTR) of between 0.0g per 100 sq. inches per 24 hours and 0.2 g per 100 sq. inches per 24 hours. In some embodiments, the packaging has an oxygen transmission rate (OTR) of between 0.0001-1 cubic centimeters per 100 sq. inches per 24 hours. In some embodiments, the packaging is constructed from a material having an oxygen transmission rate of between 0.0001-1 cubic centimeters per 100 sq. inches per 24 hours. In some embodiments, the packaging has an oxygen transmission rate (OTR) of between 0.0005-0.06 cubic centimeters per 100 sq. inches per 24 hours. In some WO 2024/168313 PCT/US2024/015273 embodiments, the packaging is constructed from a material having an oxygen transmission rate of between 0.0005-0.06 cubic centimeters per 100 sq. inches per 24 hours. In some embodiments, the packaging has an oxygen transmission rate (OTR) of 0.06 cubic centimeters per 100 sq. inches per 24 hours, or lower. In some embodiments, the packaging is constructed from a material having an oxygen transmission rate of 0.06 cubic centimeters per 100 sq. inches per 24 hours, or lower. In some embodiments, the packaging has an oxygen transmission rate (OTR) of less than 0.001 cubic centimeters per 100 sq. inches per 24 hours. In some embodiments, the packaging is constructed from a material having an oxygen transmission rate of less than 0.001 cubic centimeters per 100 sq. inches per 24 hours. OTR values described herein are measured at 65% relative humidity and 20 degrees Celsius. [00205]Methods for improving plant health [00206]In some embodiments, the invention provides methods of improving plant health comprising heterologously disposing one or more microorganisms to a plant element in an effective amount to increase a trait of agronomic importance in the plant derived from the treated plant element relative to a plant derived from a reference plant element. In some embodiments, the one or more microorganisms are a component of a treatment formulation. In some embodiments, the one or more microorganisms are a component of a synthetic composition. [00207]In some embodiments, the invention provides methods of improving plant health comprising creating any of the synthetic compositions described herein, wherein the synthetic composition comprises any of the plant elements of any of the plants described herein and any of the one or more microorganisms described herein. In some embodiments, the synthetic composition comprises any of the treatment formulations described herein and any of the one or more microorganisms described herein. In some embodiments, the synthetic composition additionally comprises a growth medium or growth environment. A growth environment is a natural or artificially constructed surrounding capable of supporting the life of a plant. In some embodiments, the growth medium is soil. In some embodiments, the growth medium is a culture fluid suitable for propagation of a microorganism or plant tissue culture. In some embodiments, the method comprises a step of applying the synthetic composition to a growth medium. In some embodiments, the synthetic composition is applied before one or more plant elements are placed in or on the growth medium. In some embodiments, the synthetic composition is applied after one or more plant elements are placed in or on the growth medium. In some embodiments, the WO 2024/168313 PCT/US2024/015273 method comprises a step of germinating the plants. In some embodiments, the method comprises a step of growing the plants. In some embodiments, the method comprises a step of growing the plants to maturity. In some embodiments, where the plants are commercially produced, maturity is the stage at which the plant is normally harvested. [00208]In some embodiments of any of the methods described herein, plant health may be improved for plants in a stress condition. In some embodiments, the stress condition is a biotic or abiotic stress, or a combination of one or more biotic or abiotic stresses. In some embodiments of any of the methods described herein, the stress condition is an abiotic stress selected from the group consisting of: drought stress, salt stress, metal stress, heat stress, cold stress, low nutrient stress (alternately referred to herein as nutrient deficiency or growth in nutrient deficient conditions), and excess water stress, and combinations thereof. In some embodiments of any of the methods described herein, the stress condition is a biotic stress selected from the group consisting of: insect infestation, nematode infestation, complex infection, fungal infection, bacterial infection, oomycete infection, protozoal infection, viral infection, herbivore grazing, and combinations thereof. Stress tolerance is exemplified by improvement of one or more other traits of agronomic importance when compared with a reference plant, reference plant element, or reference population. For example, biotic stress tolerance may be shown by decreased pathogen load of tissues, decreased area of chlorotic tissue, decreased necrosis, improved growth, increased survival, increased biomass, increased shoot height, increased root length, etc. relative to a reference.

Examples Example 1. Isolation and identification of microorganisms id="p-209" id="p-209"

[00209]Microorganisms of the present invention were isolated as described in Table 3and Table 4 Table 3. Sources of microbes of the present invention MICIDT axonomy Isolated FromMIC-28837 Pseudomonas oryzihabitans Thespesia populneaMTC-70076 Kosakonia cowanii Zea maysMIC-82330 Kosakonia cowanii Secale cerealeMIC-38013 Streptomyces kathirae Zea maysMIC-99849 Curtobacterium citreum Oryza saliva WO 2024/168313 PCT/US2024/015273 MICIDMIC-14459T axonomyBrucella anthropiIsolated FromZea mays Table 4. Method of isolating microbes of the present invention MIC Isolation Method ID MIC- Seeds of Thespesiapopulnea trees were collected at Luquillo, Puerto Rico and Rincon, Puerto Rico.28837 Thespesia populnea seeds were surface sterilized with 4% NaOCI for 20 minutes, rinsed three timeswith sterile water, placed on potato dextrose agar (PDA) plates and incubated at room temperature for hours.MIC- Zea mays L. subsp. mays Kokoma (landrace maize) PI 213733 seeds were obtained from USDA North 70076 Central Regional PI Station,, seeds were surface sterilized and isolated via dilution plating on R2A.MIC- Wrens Abruzzi Winter Rye, Secale cereale were surface sterilized and isolated via dilution plating. 82330MIC- Oryza nivara seeds were obtained from GSOR 311699 Rice Genetic Stock Center, Dale Bumpers99849 National Rice Research Center, USDA, and the seed surface rinsed in sterile water and MIC-99849isolated via dilution plating.MIC- Zea mays Ames 8563 seeds were obtained from North Central Regional PI Station, USDA, ARS, and, ,, the seed surface rinsed in sterile water and MIC-14459 isolated via dilution plating.14439 1 6 Phylogenetic and Genomic Analysis of Microorganisms id="p-210" id="p-210"

[00210]Phylogenetic and genomic analyses for bacterial strains. According to the manufacturer’s protocol, DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit with a final elution volume of 60^1 (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using a Qubit fluorometer (ThermoFisher Scientific, Waltham, MA) and normalized to 100 ng. DNA was prepared using the Nextera DNA Flex Library Prep Kit according to the manufacturer’s instructions (Illumina Inc., San Diego, CA). DNA libraries were quantified via qPCR using the KAPA Library Quantification kit (Roche Sequencing and Life Science, Wilmington, MA) and combined in equimolar concentrations into one 24-sample pool. Libraries were sequenced on a MiSeq using pair-end reads (2x200bp). Reads were trimmed of adapters and low-quality bases using Cutadapt (version 1.9.1) and assembled into contigs using MEGAHIT (version 1.1.2) (Li, D., Liu, C.-M., Luo, R., Sadakane, K., and Lam, T.-W. 2015. MEGAHIT; an ultra-fast single-node solution for large and complex metagenomics WO 2024/168313 PCT/US2024/015273 assembly via succinct de Bruijn graph. Bioinformatics. 31:1674-1676). Reads were mapped to contigs using Bowtie2 (version 2.3.4) (Langmead, B., and Salzberg, S. L. 2012. Fast gapped-read alignment with bowtie 2. Nat Methods. 9 Available at: doi.org/10.1038/nmeth. 1923.), and contigs were assembled into scaffolds using BESST (2.2.8) (Sahlin, K., Vezzi, F., Nystedt, B., Lundeberg, J , and Arvestad, L. 2014. BESST-efficient scaffolding of large fragmented assemblies. BMC bioinformatics. 15:281). [00211]Genes for phylogenetic analyses were extracted from genome assemblies using barrnap (Seemann, T. 2019. barmap 0.9: rapid ribosomal RNA prediction. Available at: github.com/tseemann/barmap ) orblast (Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research. 25:3389-3402). Homologous DNA sequences from types or other, likely correctly identified strains were retrieved from GenBank and aligned using MAFFT (Katoh, K., and Standley, D. M. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution. 30:772-780), or other software. Single or multilocus phylogenetic analyses were performed using PAUP (Swofford, D. L. 2002. PAUP* Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sunderland, Massachusetts: Sinauer Associates) or similar software. [00212]16S rRNA gene sequences were extracted from genome assemblies using barrnap (Seemann 2019). Phylogenomic analyses were performed using GToTree (Lee, M. D. 2019. Applications and considerations of GToTree: a user-friendly workflow for phylogenomics. Evolutionary Bioinformatics. 15:1176934319862245) with default settings. Average nucleotide identity analyses were performed using the pyani ANIm algorithm (Richter, M., and Rossello- Mora, R. 2009. Shifting the genomic gold standard for the prokaryotic species definition. Proceedings of the National Academy of Sciences. 106:19126-19131) implemented in the MUMmer package (Kurtz, S., Phillippy, A., Delcher, A. L., Smoot, M., Shumway, M., Antonescu, C., et al. 2004. Versatile and open software for comparing large genomes. Genome biology. 5:R12) retrieved from github.com/widdowquinn/pyani . [00213]Identification of bacterial strains. A bacteria is identified at the species level, if: its average nucleotide identity (ANI) was >95% to the genome of a single species represented by its type strain downloaded from GenBank. Phylogenomic analyses were also performed if a bacteria WO 2024/168313 PCT/US2024/015273 had >1 species with >95% ANI, or the gap between the top two ANI hits was < 3%, in this case, the bacteria is identified at the genus and species if it had a single sister group with > 70% bootstrap support. [00214]Genome sequencing and phylogenetic analyses for fungal strains. For fungal strains, DNA extractions were performed using the ZYMO Quick-DNA Fungal Bacterial 96 Kit (D6006, Zymo Technologies). Approximately 30-50 mg of hyphal material are lysed in a GeneGrinder Tissue Homogenenizer, at 3500 rpm for 3 minutes. The extraction protocol follows recommended by the manufacturer. DNA samples were quantified using a Qubit fluorometer (ThermoFisher Scientific, Waltham, MA) and normalized to 100 ng. For sequencing, DNA was prepared using the NEXTFLEX Rapid XP DNA-Seq Kit HT (Perkin Elmer), and sequenced in pools of 96 samples, on a NovaSeq 6000 using an SP flow cell, generating 150 bp paired-end read libraries. Reads were trimmed of adapters and low-quality bases using Cutadapt and assembled into contigs using MEGAHIT (version 1.1.2) (Li, D., Liu, C.-M., Luo, R., Sadakane, K., and Lam, T.-W. 2015. MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics. 31:1674-1676). Reads were mapped to contigs using Bowtie2 (version 2.3.4) (Langmead, B., and Salzberg, S. L 2012. Fast gapped-read alignment with bowtie 2. Nat Methods. 9), and contigs were assembled into scaffolds using BESST (2.2.8) (Sahlin, K., Vezzi, F., Nystedt, B., Lundeberg, J., and Arvestad, L. 2014. BESST-efficient scaffolding of large fragmented assemblies. BMC bioinformatics. 15:281). [00215]Genes for phylogenetic analyses were extracted from genome assemblies using barrnap (Seemann, T. 2019. barrnap 0.9: rapid ribosomal RNA prediction. Available at: github.com/tseemann/barrnap ) orblast (Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. 1997. Gapped BLAST and PSI-BLAST; A new generation of protein database search programs. Nucleic Acids Research. 25:3389-3402). Homologous DNA sequences from types or other, likely correctly identified strains were retrieved from GenBank and aligned using MAFFT (Katoh, K., and Standley, D. M. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution. 30:772-780), or other software. Single or multilocus phylogenetic analyses were performed using PAUP (Swofford, D. L. 2002. PAUP*. Phylogenetic Analysis Using Parsimony WO 2024/168313 PCT/US2024/015273 (* and Other Methods). Version 4. Sunderland, Massachusetts: Sinauer Associates) or similar software. [00216]Identification of fungal strains. A fungal strain is identified at the species level, if: it had a single sister group with > 70% bootstrap support; identification may be confirmed by morphological comparison to related species. [00217]Identification of microorganisms by sequencing of marker genes. The microorganisms were characterized by the sequences of genomic regions. Primers that amplify genomic regions of the microorganisms of the present invention are listed in Table 5.Sanger sequencing was performed at Genewiz (South Plainfield, NJ). Raw chromatograms were converted to sequences, and corresponding quality scores were assigned using TraceTuner v3.0.6beta (US 6,681,186). These sequences were quality filtered, aligned and a consensus sequence generated using Geneious v 8.1.8 (Biomatters Limited, Auckland NZ). The consensus sequences identifying exemplary microorganisms have SEQ IDs 5-122.

Table 5. Primer sequences useful in identifying microbes of the present invention SeqID Primer27f׳ 14921 2515f806r Description________________ Sequence__________16S ribosomal RNA AGAGTTTGATYMTGGCTCAG16S ribosomal RNA GGTTACCTTGTTACGACTT16S ribosomal RNA GTGYCAGCMGCCGCGGTAA16S ribosomal RNA GGACTACNVGGGTWTCTAAT Example 2. Production of microbial treatments id="p-218" id="p-218"

[00218]Preparation of microorganism biomass; Approximately 0.5 ml cryopreserved culture was transferred via pipette into 50-100 ml media in a 125-250 ml seed culture flask with a baffled bottom and aerated lid. The seed flask was incubated at 24C or 30C for a period of 24h to days (depending on the microbial strain). While seed flasks were growing, bioreactors were batched with appropriate growth medium. Following incubation, seed flasks were checked for purity via microscopic examination and used to inoculate bioreactors (at a rate of 0.1-10%).Bioreactors were run with conditions appropriate for the organism, generally at a pH of 5-7, a temperature of 24-37°C, and an elapsed fermentation time of 2411 to 7 days. Bioreactors were then harvested and biomass concentrated to a concentrate (typically 8-30X) via centrifugation or tangential flow filtration.

WO 2024/168313 PCT/US2024/015273 Example 3. Preparation of RTU formulations Water dispersedRTUformulation id="p-219" id="p-219"

[00219]Water dispersed endophyte formulations comprise microorganism biomass in liquid fermentation broth that may be diluted in a buffered carrier such as phosphate buffered saline as well as a preservative and/or a pH adjusting agent. Water dispersed RTU formulation comprising MIC-2883 7 was prepared for the formulation stability assay (results shown in FIG. 2) at an initial concentration of 1E+09 CFU/mL. When applied to seeds, the volume of seeds was used to determine the volume of endophyte in water dispersion formulation needed for the target dose per seed. A target dose of 1E+05 CFU/seed was prepared for the on-seed stability assay (results shown in FIGs. 3A and 3B). The calculated volume of endophyte formulation was added to the seeds in a clean mixing vessel. The seeds and endophyte formulation were mixed for at least seconds to ensure the endophyte formulation was well dispersed on the seeds. Water dispersed formulations comprising viable microbial biomass may be prepared in any concentration suited for the purpose, for example some water dispersed formulations for agricultural applications may comprise microorganisms at a concentration of between about 1.0E+06 to 3.0E+09 CFU/mL.

Oil dispersedRTUformulations id="p-220" id="p-220"

[00220]Oil dispersion formulations comprise microorganism biomass, a vegetable oil-based carrier, and one or more of a dispersant, and/or a rheology modifier. Oil dispersion formulations comprising viable microbial biomass may be prepared in any concentration suited for the purpose, for example some RTU formulations for agricultural applications may comprise microorganisms at a concentration of between about 1.0E+06 to 1.0E+08 CFU/mL.

Flowable powder RTU formulations id="p-221" id="p-221"

[00221]Flowable powder microorganism formulations comprise talc, mineral oil base, and spray dried, lyophilized, or solid-state fermentation produced endophyte. A. target dose of 1E+CFU/seed was prepared for the on-seed stability assay (results shown in FIGs. 3A and 3B).Flowable powder formulations comprising viable microbial biomass may be prepared in any concentration suited for the purpose, for example some flowable powder formulations for agricultural applications may comprise microorganisms at a concentration of between about 1.0E+06 to 3.0E+09 CFU/g.69 WO 2024/168313 PCT/US2024/015273 Example 4. Preparation of dried powder intermediate compositions Spray dried intermediate compositions id="p-222" id="p-222"

[00222]This example describes a method for producing a spray dried powder intermediate product (MUP) used for shipping and storage stability of biological compositions. [00223]Cell Concentration. Concentration of biological material (technical grade active ingredient, TGAI) was confirmed using a liquid viability protocol consisting of: 1) making series of serial dilutions from the whole culture produced in Example 2, using a buffer at physiological pH (e.g. PBS, 50mM MOPS-Buffered Saline, Tris) as a diluent, and 2) plating lOOpl from the target dilutions onto an appropriate sterile media, such as sterile Nutrient Agar (Table 6)or Tryptic Soy Agar petri plates, and 3) incubating plates for a time and in conditions for growth of the organism, (for example, at 20-30°C for 24-48h), and 4) counting colonies to calculate CFU/mL.

Table 6. Nutrient Agar Component name llillBeef extract68990-09-0 3Peptone73049-73-7 5Sodium chloride7647-14-5 5Agar9002-18-0 15 id="p-224" id="p-224"

[00224]A cell concentrate (for example, 10X) was then produced, either by batch centrifugation or continuous centrifugation or tangential flow filtration. If using batch centrifugation, the fermentation broth from Example 2 was centrifuged at 5,000-15,000 X g for 15-20 minutes at 4- 20°C. Ninety percent of the original volume was decanted and the pellet resuspend in the remaining supernatant by vortexing or shaking until the concentrate appeared homogenous. The Liquid Viability Protocol described above was used to confirm lOx microbial concentration. If using continuous centrifugation or tangential flow filtration (TFF), lOx concentration of effluent is targeted based on volume and confirmed using the Liquid Viability Protocol described above. [00225]Feedstock Preparation. Feedstock was prepared by adding the components of the feedstock listed in Table7 or 8as follows. The required water was added to a mixing tank equipped with a mixer. The required water is the mass of water needed to bring the sum of components in the feedstock to 1 kg. While mixing, the ascorbic acid and sodium bicarbonate WO 2024/168313 PCT/US2024/015273 were added and mixed for at least 10 minutes (or longer if required for complete dissolution by visual inspection). pH is adjusted to about between 6.5 - 7. The maltodextrin and additional excipients specified by formulation recipe (cysteine, peptone, casamino acids, sorbitol, trehalose, glutathione, or whey) were added and mixed for at least 10 minutes or longer as required for completed dissolution by visual inspection. The pH of the formulation was measured and adjusted to 6.5-7.0 with sodium bicarbonate and/or ascorbic acid, and the mass calculated, and final pH were recorded. The final pH was measured after 10 minutes to ensure the final pH was stable. The kaolin clay and microbial concentrate were stirred into the spray dry formulation, and stirred for at least 10 minutes before proceeding to the spray drying process. The resulting mixture of cells and formulation is referred to as the spray drying feedstock. The amount of microbial concentrate is determined to achieve the desired final concentration, for example between about 1.00E+03 - 2.00E+11 CFU/mL in the feedstock. [00226]The order mixed will not affect survival and/or stability as long as all of the acidic and alkaline ingredients are mixed and pH adjusted to 6.5-7.0 before the microbial concentrate is added. pH adjustment to 6.5 - 7.0 is important to be performed before addition of the microbial concentrate to prevent negative impacts to cell health and viability. Mixing of the other components may be performed in any order, though is preferred to mix components that will release CO2 and bubble, such as sodium bicarbonate and ascorbic acid, before other components.

Table 7. Spray drying feedstock components. Component quantities listed as grams per kilograms of spray dry ing feedstock.

Component CAS F27 F31 F32 F34 Alternative Embodiments g/kg Water 7732-18-5188-488 443-143 173-473 140-44065-603 g Ascorbic Acid50-81-7 15 15 15 3010-50Sodium bicarbonate144-55-8 7 7 7 157-30Maltodextrin (low DE~4)9050-36-6 130100-150Maltodextrin (mid DE-IO)9050-36-6 130 130100-150Maltodextrin (high DE-18)9050-36-6 130100-150 Peptone68607-88-5 60 50 60 6030-80 WO 2024/168313 PCT/US2024/015273 Table 8. Exemplary spray drying feedstock components for MIC-28837 Component CAS F27 F31 F32 F34 Alternative Embodiments g/kg Casamino acids65072-00-6 1510-80 Sorbitol50-70-4 2010-100 Trehalose6138-23-4 20 1510-100 Cysteine52-90-4 255-25Adjust to pH 6.5-7.0, e.g. with sodium bicarbonate or ascorbic acidKaolin Clay1332-58-7 100 100 100 10050-100Microbial concentrateNA 200-500 200-500 200-500 200-500200-500 Component CAS F34 for MIC- 28837 g/1 kg composition Water 7732-18-5363 Ascorbic Acid50-81-7 25-30 Sodium bicarbonate144-55-8 7-15 Maltodextrin (DE~10)9050-36-6 130 Peptone68607-88-5 60 Cysteine52-90-4 15-25Adjust to pH 6.5- 7.0, e.g. with sodium bicarbonate or ascorbic acidKaolin Clay1332-58-7 100 Microbial concentrateNA 300 id="p-227" id="p-227"

[00227]Spray Drying. The spray drying feedstock was constantly mixed during spray drying to prevent settling of insoluble components. The concentration of spray drying feedstock was confirmed using the Liquid Viability Protocol. The spray drying parameters in Table 9were used.

WO 2024/168313 PCT/US2024/015273 Table 9. Spray drying parameters Parameter Setpoint Inlet Temperature100°C (for example, 75OC - 140oC)Target Outlet Temperature60°C (for example, 45°C-65°C) id="p-228" id="p-228"

[00228]The spray dryer was run to steady state without feedstock, before starting to feed water and again waiting until steady state was reached. Then the spray drying feedstock was begun and maintained to ensure constant feeding to the spray dryer. The final concentration of the spray dried powder was determined using the following Powder Viability Assay: 0.04 to 0.8 g of spray dried powder was weighed into a microcentrifuge tube and the actual weight added was recorded. One mL of sterile 50mM MOPS-Buffered Saline was added to the powder and vortexed for > 5 minutes to ensure the powder was dissolved. Serial dilutions were prepared from the dissolved powder and lOOpl from the target dilutions was plated onto sterile Nutrient Agar petri plates, in triplicate. The plates were incubated at temperatures and conditions suitable for growth of the relevant organisms (for example, 30°C for 24-48h), and colonies counted to calculate CFU/g of the spray-dried powder. [00229]Survival through spray drying process was determined by comparing the concentration of biologically active ingredients in the spray dried powder to the concentration of biologically active ingredients within the feedstock and calculating a precent survival by determining the percentage of biologically active ingredients within the feedstock detectable in the spray dried powder. To calculate the survival percentage, the liquid feedstock active ingredient concentration by volume is converted to colony forming units per the mass of solids in the feedstock which is then compared to colony forming units per mass in the final dried product. Results of survival through the spray drying process are shown in Table 10for a set of taxonomically diverse organisms.

Table 10. Spray drying survival of representative microorganisms.

GN = Gram negative bacteria, F =fungi, GPNS = Gram positive non-spore former. Survival score of 1 represents excellent survival (>75%). A survival score of represents good survival (40-75%). A survival score of 3 represents fair survival (20-40%).

SUBSTITUTE SHEET (RULE 26) WO 2024/168313 ___, ___, ___, ___ , ___ , ___, ___, ___, ___, ___, ____ , ___, ___, ___, ___PCT/US2024/015273 Group Taxonomy Family Order Class Phylum/Division Formulation code Survival score F Coniochaeta elegans Coniochaetaceae Coniochaetales Sordariomycetes Ascomvcota F27 2F Coniochaeta endophytica Coniochaetaceae Coniochaetales Sordariomycetes zlscomycota F31 2F Coniochaeta ligniara Coniochaetaceae Coniochaetales Sordariomycetes Ascomvcota F31 1F Occullifur kilboumensis Cystobasidiaceae Cystobasidiales Cystobasidiomyceles Basidiomycota F31 1GN Rahnella aceris Enterobacteriaceae Enterobacterales Gammapro teobac teria Proteobatcerla F34GN Rahnella aceris Enterobacter iaceae En ter abac ter ales Gumm a pro teo bac teria Proteobalceria F27 1GN Bradyrhizobium diazoefficiens Nitrobacteraceae Hyphom icrobiales Alphaproteobacteria Pseudomonadota F27 1GN Bradyrhizobium diversitatis Nitro bacteraceae Hyphom icrobiales A Iphapro teobacteria Pseudomonadota F31 2GN Bradyrhizobium elkanii Nitrobacteraceae Hyphom icrobiales Alphaproteobacteria Pseudomonadota F27 1GN Azorhizobium caulinodans Xanthobacteraceae Hyp ho m icrobiales Alphaproteobacteria Pseudomonadota F27 1 GNBrucella anthropi (M1C-.14459) Britcellaceae Rhizobiales Alphaproteobacteria Pseudomonadota F27 2GN Azospirillum baldaniorum Azospirilfaceae Rhodospiri Hales A iphapro teobacteria Pseudomonadota F31 1GN Sphingomonas yabuuchiae Sphingomonadaceae Sph ingomonadales Alphaproteobacteria Pseudomonadota F27 1GN Herbaspirillum seropedicae Oxalobacteraceae Bwkholderiales Betaproteobacteria Pseudomonadota F31 3GN Enterobacter asburiae Enterobacteriaceae En terobac ter ales Gammaproteobac teria Pseudomonadota F27 1GN Enterobacter roggenkampii Enterobacteriaceae Enterobacterales Gammaproteobacteria Pse udomo nadota F27 2 GNKosakonia cowanii (MIC-70076) Enterobacteriaceae Enterobacterales Gammapro teobac teria Pseudomonadota F27 2 GNKosakonia cowanii (MIC-K2330) Enterobacteriaceae Ente ro bac ter ales Gammaproteobacteria Pseudomonadota F27 2GN Rahnella aceris Enterobacteriaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 2GN Siccibacter colletis E nterobacteriaceae E n terobacterales Gammaproteobacteria Pseudomonadota F27 1 SUBSTITUTE SHEET (RULE 26) Group Taxonomy Family Order Class Phylum/Division Formulation code Survival score GN Pantoea allii Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27GN Pantoea eucalypti Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F31GN Pantoea eucrina Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 2GN Pantoea eucrina Erwiniaceae Enterobacterales Gammapro teobac ter la. Pseudomonadota F27 1GN Pseudomonas b ij teens is Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 2GN Pseudomonas brassicaceamm Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 1GN Pseudomonas fulva Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 1GN Pseudomonas glycinis Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 1GN Pseudomonas oryzihabitans Pseudomonadaceae Pseudomonadales Gammapro teobac teria Pseudomonadota F27 1GN Pseudomonas oryzihabitans Pseudomonadaceae Pseudotnonadales Gammaproteobacteria Pseudomonadota F31 1 GNPseudomonas oryzihabitans (MIC-28837) Pseudomonadaceae Pseudomonadales Gam a! tro teobac teria Pseudomonadota F27 1GN Pseudomonas rhodesiae Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F31 2GN Pseudomonas straminea Pseudomonadaceae Pseudomonadales Gammapro teobac. teria. Pseudomonadota F27GN Stenotrophomonas pavanii Xanthomonadaceae Xanthomonadales Gamm apro teobac teria Pseudomonadota F27 1 GPNSCurtobacterium citreum (MIC-99849) Microbacteriaceae Micrococcales A ctinomycetia A ctinomycetota F27 2 SYM Group Taxonomy Genus Family Order Class Phylum/Division Formulati on code Survival score 16970 F Coniochaeta elegans Coniochaeta Coniochaetaceae Coniochaetales Sordariomycetes Ascomycota F27 hS 19040 F Coniochaeta endophytica Coniochaeta Con iochae taceae Coniochaetales Sordariomycetes Ascomycota F3116932 F Coniochaeta figniara Coniochaeta Coniochaetaceae Coniochaetales Sordariomycetes Ascomycota F31)/כ41850 F Occultifur kilbournensis Occultifur Cystobasidiaceae Cystobasidiales Cys tobasidiomyce tes Basidiomycota F31 K) 4-* 48573 GN Rahnella aceris Rahnella Enterobacteriaceae Enterobacterales Gammaproteobacteria Proteobatceria F34 a .. 1—k LA 46836 GN Rahnella aceris Rahnella Entero bacteriaceae Enterob acterales Gammaproteobacteria Proteobatceria F27 04 SUBSTITUTE SHEET (RULE 26) 37910 GNBradyrhizobium diazoefficiens Bradyrhizobium Nitrobacteraceae Hyphomicrobiales Alphaproteobacteria Pseudomonadota F2747849 GN Bradyrhizobium diversitatis Bradyrhizobium Nitrobacteraceae Hyphomicrobiales Alphaproteobacteria Pseudomonadota F31 © 41995 GN Bradyrhizobium elkanii Bradyrhizobium Nitrobacteraceae Hyphomicrobiales Alphaproteobacteria Pseudomonadota F27 o bU 4-* 38051 GN Azorhizobium caulinodans Azorhizobium Xan thobacteraceae Hyphomicrobiales Alphaproteobacteria Pseudomonadota F27 1—k 596 GNBrucella anthropi (MIC- 14459) Brucella Brucellaceae Rhizobiaies Alphaproteobacteria Pseudomonadota F27 1—k U4 39122 GN Azospirillum baldanlorum Azospirillum Azospirillaceae Rhodospirillales Alphaproteobacteria Pseudomonadota F31 137338 GN Sphingomonas yabuuchiae Sphingomonas Sphingomonadaceae Sphingomonadales Alphaproteobacteria Pseudomonadota F2729062 GN Herbaspirillum seropedicae Herbaspirillum Oxalobacteraceae Burkholderlales Betaproteobacteria Pseudomonadota F31 344207 GN Enterobacter asburiae Enterobacter Enterobacteriaceae Enterob acterales Gammaproteobacteria Pseudomonadota F27 150649 GN Enterobacter roggenkampii Enterobacter Enterobacteriaceae Enterobacterales Gammapro teobacteria Pseudomonadota F27 2 28 GNKosakonia cowanii (MIC- 70076) Kosakonia Enterobacteriaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 2 175 GNKosakonia cowanii (MIC- 82330) Kosakonia Enterobacteriaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 2 O 49913 GN Rahnella aceris Rahnella Enterobacteriaceae Enterob acterales Gammaproteobacteria Pseudomonadota F27 249437 GN Siccibacter colletis Siccibacter Enterobacteriaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 144098 GN Pantoea allii Pantoea Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 226930 GN Pantoea eucalypti Pantoea Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F31 136690 GN Pantoea eucrina Pantoea Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 244081 GN Pantoea eucrina Pantoea Erwiniaceae Enterobacterales Gammaproteobacteria Pseudomonadota F27 144235 GN Pseudomonas bijieensis Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 2 32586 GNPseudomonas brassicacearum Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 hd n 29848 GN Pseudomonas fulva Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 a 34326 GN Pseudomonas glycinis Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 b□ o b□ 28967 GN Pseudomonas oryzihabitans Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 4-* o 36735 GN Pseudomonas oryzihabitans Pseudomonas Pseudomonadaceae Pseudomonadales Gam m apro teobacteria Pseudomonadota F31Ea b□ 23945 GNPseudomonas oryzihabitans (MIC-28837) Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F2739747 GN Pseudomonas rhodesiae Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F3139224 GN Pseudomonas straminea Pseudomonas Pseudomonadaceae Pseudomonadales Gammaproteobacteria Pseudomonadota F27 37802 GN Stenotrophomonas pavaniiStenotrophomo nas Xan thomonadaceae Xanthomonadales Gammaproteobacteria Pseudomonadota F27 525 GPNSCurtobacterium citreum (MIC-99849) Curtobacterium Microbacteriaceae Micrococcales Actinomycetia Actinomycetota F27 SUBSTITUTE SHEET (RULE 26) W O 2024/168313 N PCT/US2024/015273 WO 2024/168313 PCT/US2024/015273 Lyophilized intermediate compositions id="p-230" id="p-230"

[00230]Lyophilization. Lyophilization feedstocks are prepared as defined in Table 6with the omission of kaolin clay. Additional water can be added to the composition to decrease the pre- lyophilization feedstock buffer total solids, but the final powder composition will not be changed. The feedstock is first frozen at -50°C to -30°C for a minimum of 4 hours. Primary drying occurs under vacuum as either a ramped temperature or static cycle between -40°C and 0°C until greater than 90% of the water is sublimated. Secondary drying is then ramped up to 20°C and proceeded until the lyophilized cake has a moisture content less than or equal to 5%. The vacuum is broken with ambient air and the lyophilized cake is removed and pulverized manually or milled.

Packaging and storage (optional) id="p-231" id="p-231"

[00231]Optionally the powder intermediate product is stored in a heat sealed pouch form, containing desiccant packs approximately 20% of the mass of the spray dried powder. The powders may be stored at refrigeration temperatures between (4-10°C) or at room temperature.

Example 5. Procedure for determining concentration of microorganisms Plating spray-dried and lyophilized powders id="p-232" id="p-232"

[00232]This example describes an exemplary method for weighing powder synthetic compositions (for example, spray-dried or lyophilized powders) and resuspending powders into a slurry and plating for CFU. [00233]First, 0.0500 g +/- 0.0200 g of powder synthetic compositions were aseptically added to a sterile microcentrifuge tube, and the weight of the powder recorded. One mL of sterile buffer (alternate buffers include MOPS, PBS, tris, etc.) was added to the pre-weighed tubes. The tubes were vortexed at high speed for 5-15 minutes. The powder slurry was serially diluted within minutes of resuspending the powder and plated on agar plates for CFU within one hour of the PBS addition. Agar plate types could include TSA, PDB, NA, R2B, etc. depending on the organism. Plates were incubated at room temperature to 37°C for 1-5 days, colonies were counted, and CFU/g was calculated. The concentration of microorganisms in the MUP powders WO 2024/168313 PCT/US2024/015273 was measured at repeated intervals by isolating and plating samples collected from the stored samples. Results are shown in Table 10, Table 11,and FIG. 2.

Water dispersed samples id="p-234" id="p-234"

[00234]Water dispersed samples, also referred to as liquid samples, were serially diluted and plated on agar plates for CFU. Agar plate types could include TSA, PDB, NA, or R2B, depending on the organism. Plates were incubated at room temperature to 370C for 1-5 days, colonies were counted, and CFU/g was calculated. Results are shown in FIGs. 2, 3A, 3B, 4A, 4B, 5, 6,and 7.

On seed id="p-235" id="p-235"

[00235]Viability of RTU compositions on seeds was determined by the following methods. A3- gram sample was collected from stored samples of RTU treated seed and placed in a 50 mL falcon tube. 10 mL of sterile buffer (e.g. IX PBS) was added to each tube and the cap secured. The tubes were then vortexed for 30 minutes, the vortex was observed to ensure that all seeds in the falcon tubes were moving freely. Three 1 mL samples of the solution were transferred to a 96-well plate and plated for CFU as described above. Results are shown in FIGs. 3A, 3B, 4A, 4B, 5, 6,and 7.

Table 11 Predicted time to 1 log loss of representative microorganisms stored at 4C, room temperature (RT), and 35C. O The numbers in brackets indicate the day on which the most recent measurement occurred (where the day is the number of days post spray drying). If the צpredicted days to 1 log loss (no brackets) is less that the number of days in the last measurement (brackets) the number without brackets represents the observed it number of days to 1 log loss. GN = Gram negative bacteria, F = fungi, GPNS = Gram positive non-spore former.

SUBSTITUTE SHEET (RULE 26) Group T axoiiomy Family Order Class Phylum / Division Form, code Survival score Predicted days to 1 log loss !days measured | 4C RT 35C GPNS Curtobactenum g 1treum Microbaclcriaceae Micrococcales A cnnomycefia Actiuomycetoia F27 2 >1000 [366j 721 [366] 398 [366]F Coniochaeta elegants Coniochaetaceae Comochaelales Sordariomycetes Ascomycota F27>1000 [181] 449 [181] 587 [181]F Contochaeta endophytica Coniochaetaceae Contochaetales Sordartomycetes Ascomycola F31>1000 [179] 877 [179] >1000 [179]F Coniochaeta Honiara Con i ochaetaccae Coniochaetaies Sordariomycetes Ascomycota F31 1 >1000 [188] >1000 [188] >1000 [188]F Occultifur kilbournensis Cystobasidiaceae Cystobasidiales Cyslobasidiomyceles Basidiomycota F31 1 >1000 [272] 832 [272] 486 [272]GN Rahnella aceris Enterobaereriaceae Enterobact erules Gammaproteobacleria Proteobaf ceria F34 2 416 [63] 209 [63] 32 [63]GN Bradyrhizobium diversitatis Ni trobacleraceae Hyphomicrobiules Alphaproteobacteria Pseudomonadota F31 2 >1000 [28] 94 [28] 25 [28]GN Brucella anthropi (MIC-14459)Brucellaceae Rhizobiaies A Iphuproteobucterkt Pseudomonadota F27 2 839 [365] 194 [365] 44 [365]GN Azckcpirillum baldoniornm A 20 sp trillaceae Rhodospiri Hales Alphaproteobacteria Pseudomonadota F31 1 >1000 [182] 678 [182] 473 [1 82]GN Sphingomonas ycfbmichiae Sp hingomonadaceae Sphingomonadaies A Iphaproteobacteria Pseudomonadota F27 1 >1000 [272] 224 [272] 77 [272]GN Herbaspirilhim seropedicae Oxalo bacteraceae Burkholderiales Betaproteobacteria Pseudomonadota F31 3 396 [365] 204 [365] 33 [365]GN Kosakonia coxeanii 6W2-70076)Enterobucteriaceae Enterobaclerules Gammaproteobacleria Pseudomonadota F27 2 >1000 [378] 545 [378] 83 [378]GN Kosakonia cowunii (MIC-82330)Enlerobacreriaceae Enterobaclerules Gammaproteoba cl eri a Pseudomonadota F27 2 >1000 [365] 462 [365] 266 [365]GN Rahnella aceris Enlerobacteriaceae Ente roboelerales Gammaproteobacleria Pseudomonadota F27 2 >1000 [361] I 30 [361 ] 40 [361]GN Siccibacter coMelis Ed terobacteri ac eae E n te ro b a c te ra J es Gammaproteobacteria. Pseudomonadota. F27 1 >1000 [275] 541 [275] 149 [275] hdGN Pantoea eucalypti Erwi n iaceae E n te robaeierales G am maproteoba cle ria Pseudomonadota F31 1 >1000[64] 562 [64] 491 [H HGN Pseudomonas glycinis Pse u do mo nad aceae Pseudomonadales G am maproteob a cle ria Pseudomonadota F27 1 781 [272] 1 19 [272] 59 [2aGN Pseudomonas oiyzihabiUms Pseudomonadaeeae Pseudomonadales Gammaproteobacleria Pseudomonadota F27 1 952 [379] 516 [379] 145 [1gGN Pseudomonas oryzihabitans (MIC-28337)Pseudomonadaeeae Pseudomonadales Gammaproteobacleria Pseudomonadota F27 1 >1000 [362] 984 [362] 561GN Pseudomonas oryzihabitans Pseudomonadaeeae Pseudomonadales Gammaproteobacleria Pseudomonadota F31 1 324 [62] 328 [62] 67 [ Group Taxonomy Family Order Class Phylum / Division Form, code Survival score Predicted days to 1 log loss {days measured! 4C RT 35 GN Pseudomonas siraminea Pseudomonadaeeae Psettdomonadales Gammaproteo bacteria Psendomonadota F27 2 468 [182] 94 [182] 18 [JGN Slenotroph omonas pa. van i i Xanthomonadaceae Xanthomonadales Gammaproteabacteria Psendomonadota F27 1 >1000 [367] 344 [367] 39 [3 SUBSTITUTE SHEET (RULE 26) WO 2024/168313 PCT/US2024/015273 WO 2024/168313 PCT/US2024/015273 Example 6. Production of a ready to use powder synthetic composition suitable for treating plants. id="p-236" id="p-236"

[00236]Spray dried intermediate powder is combined with optional ingredients such as talc, mineral oil, graphite, micronutrients, fertilizers, and amino acids to obtain a target dose applicable to the intended use, for example agricultural seed treatment applications may have a concentration of microorganism of between about 1.0E+06 to 2.0E+10 CFU/g.

Example 7. Production of synthetic fertilizer compositions. id="p-237" id="p-237"

[00237]This example describes an exemplary method by which compatibility of synthetic compositions comprising microorganisms and fertilizers is evaluated. [00238]Application rates. Fertilizer compositions are granular in form. Flowable powder (FP) microorganism treatments have a target application rate of 3.6 grams flowable powder per acre. Synthetic compositions are prepared using different concentrations of microorganism and fertilizer (% w/w), representing between 5-50 times application rate of microorganism to seeds. The FP microorganism treatments are prepared as 0.01 % w/w (microorganism/fertilizer), corresponding to an application rate of 0.15 fluid oz (0.28 dry oz.) of microorganism per hundred weight of fertilizer composition, and 0.10 % w/w, corresponding to an application rate of 1.fluid oz (2.8 dry oz.) of microorganism per hundred weight of fertilizer composition. Synthetic compositions are blended and stored at either 22 °C with between 20-60% relative humidity or °C with 80% relative humidity. Viability of synthetic compositions comprising fertilizer are measured at repeated intervals (for example, 1 week, or 1-month intervals) by isolating and plating samples collected from the stored samples.

Example 8. Viability over time of microorganisms in synthetic fertilizer compositions.

This example describes an exemplary method by which stability of synthetic compositions comprising microorganisms and treatment formulations is evaluated. id="p-239" id="p-239"

[00239]Application rates. Fertilizer compositions are granular in form. Flowable powder (FP) microorganism treatments have a target application rate of 3.6 grams per acre. Synthetic compositions are prepared using different concentrations of microorganism and fertilizer (% w/w), representing between 5-50 times application rate of microorganism to seeds. The FP WO 2024/168313 PCT/US2024/015273 microorganism treatments are prepared as 0.01 % w/w (microorganism/fertilizer), corresponding to an application rate of 0.15 fluid oz (0.28 dry oz.) of microorganism per hundred weight of fertilizer composition, and 0.10 % w/w, corresponding to an application rate of 1.54 fluid oz (2.dry oz.) of microorganism per hundred weight of fertilizer composition. Synthetic compositions are blended and stored at either 22 °C with between 20-60% relative humidity or 30 °C with 80% relative humidity. Viability of synthetic compositions comprising fertilizer are measured at repeated intervals (for example, 1 week, or 1-month intervals by isolating and plating samples collected from the stored samples).

Example 9. Method of treating seeds with flowable powder synthetic composition id="p-240" id="p-240"

[00240]Flowable powder microorganism formulations comprise talc, mineral oil base and spray dried microorganism. The volume of seeds is used to determine the volume of microorganism in a powder formulation needed for the target dose per seed. The target application dose for large grain crops (e.g. corn, soy, cotton) is between about 1.0E+04 to 1.0E+05 CFU/seed. The target application dose for small grain crops (e.g. wheat, rice) is between about 1.0E+03 to 2.25E+CFU/seed The seeds to be treated are added to a clean vessel (e.g. a bucket, seed bag, seed box, seed drill, planter box, or seed tender). The calculated volume of microorganism formulation for the desired dose is added to the seeds in the clean vessel. Optionally, the microorganism formulation is mixed for at least 30 seconds to ensure the microorganism formulation is well dispersed on the seeds.

Example 10. Extender formulations for treating seeds id="p-241" id="p-241"

[00241]Compositions of the present example may be applied to a seed with a purified microbial composition close to the time of planting. The components of the composition are listed in Table 11.To prepare the extender, the ingredients listed in Table 11are thoroughly mixed and pH adjusted to between about 6.5 and 7.2. The extender may be stored in a sealed container until application, at which time the dose of microbial biomass suitable for the application (for example, for seed treatment use the quantity of microbe and seed are obtained to achieve a target application dose of between about 1.0E+3 and 1.0E+5 CFU/seed) is added to the extender and thoroughly mixed to ensure even coating of the seeds. The microbial concentrate may be in a liquid or solid (e.g. powder) format.

WO 2024/168313 PCT/US2024/015273 Table 11. Extender components. Component quantities listed percentages by wet weight.

F27 F31 F32 F34 maltodextrin — high DE (~18) 13maltodextrin — mid DE (—10) 13 13maltodextrin — low DE (~4) 13peptone 6 5 6 6casamino acids 1.5sorbitol 2ascorbic acid 1.5 1.5 1.5 3trehalose 2 1.5sodium bicarbonate 0.3 0.3 0.3 0.7cysteine 2.5water 84.1 74.3 80.8 73.7 id="p-242" id="p-242"

[00242]Having illustrated and described the principles of the present invention, it should be apparent to persons skilled in the art that the invention can be modified in arrangement and detail without departing from such principles. It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other embodiments, advantages, and modifications are within the scope of the following claims.

Claims

WO 2024/168313 PCT/US2024/015273 CLAIMS What is claimed is:

1. A formulation comprising:(a) microbial biomass;(b) one or more antioxidant in a ratio of about 0.07 to 14 by weight relative to the microbial biomass;(c) one or more pH modifier in a ratio of about 0.03 to 7 by weight relative to the microbial biomass;(d) one or more saccharide in a ratio of about 0.07 to 62 by weight relative to the microbial biomass; and(e) one or more protein hydrolysate in a ratio of about 0.07 to 29 by weight relative to the microbial biomass.

2. The formulation of claim 1, wherein:(a) the one or more antioxidant is present in a ratio of at least 0.35 by weight relative to the microbial biomass;(b) the one or more pH modifier is present in a ratio of at least 0.15 by weight relative to the microbial biomass;(c) the one or more saccharide is present in a ratio of at least 0.35 by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate is present in a ratio of at least 0.35 by weight relative to the microbial biomass.

3. The formulation of claim 1, wherein:(a) the one or more antioxidant comprises ascorbic acid and/or creatine;(b) the one or more amino acid comprises cysteine and/or glutathione;(c) the one or more pH modifier comprises sodium bicarbonate and/or sodium hydroxide;(d) the one or more saccharide comprises one or more of maltodextrin, sucrose, lactose, trehalose, and/or microcrystalline cellulose; and WO 2024/168313 PCT/US2024/015273 (e) the one or more protein hydrolysate comprises one or more of peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein.

4. The formulation of claim 1, wherein the formulation further comprises at least one inert solid present in a ratio of about 0.48 to 48 by weight relative to the microbial biomass.

5. The formulation of claim 4, wherein the at least one inert solid comprises kaolin clay, magnesium stearate, or microcrystalline cellulose.

6. The formulation of claim 1, wherein the one or more saccharide comprises maltodextrin.

7. The formulation of claim 6, wherein the maltodextrin is present at a concentration ofbetween about 0.62 to 62 by weight relative to microbial biomass.

8. The formulation of claim 6, wherein the maltodextrin is present at a concentration of at least 3.1 by weight relative to the microbial biomass.

9. The formulation of claim 1, wherein the one or more saccharide comprises maltodextrin and trehalose.

10. The formulation of claim 6, wherein the maltodextrin has a dextrose equivalent (DE) value between 4 and 20.

11. The formulation of claim 10, wherein the maltodextrin has a dextrose equivalent (DE) value of at least 8.

12. The formulation of claim 10, wherein the maltodextrin has a dextrose equivalent (DE) value of at least 10.

13. 13 The formulation of claim 10, wherein the maltodextrin has a dextrose equivalent (DE) value of at least 15. WO 2024/168313 PCT/US2024/015273

14. The formulation of claim 1, wherein the formulation further comprises one or more of a sugar alcohol, an amino acid, yeast extract, and/or whey powder.

15. The formulation of claim 14, comprising at least one amino acid present at a concentration of between about 0.12 to 12 by dry weight relative to dry microbial biomass.

16. The formulation of claim 15, wherein the at least one amino acid comprises cysteine or glutathione.

17. The formulation of claim 1, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone in a ratio of about 1.45 to by weight relative to the microbial biomass.

18. The formulation of claim 17, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.3 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone in a ratio of about 2.9 to 29 by weight relative to the microbial biomass.

19. The formulation of claim 17 or 18, wherein the maltodextrin has a dextrose equivalent (DE) value between about 6 and 14. WO 2024/168313 PCT/US2024/015273

20. The formulation of claim 1, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass and trehalose present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass;(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.to 24 by weight relative to the microbial biomass and casamino acids present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and(e) the formulation additionally comprises sorbitol present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass.

21. The formulation of claim 20, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.3 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to by weight relative to the microbial biomass and trehalose present in a ratio of about 1 to 10 by weight relative to the microbial biomass;(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 2.to 24 by weight relative to the microbial biomass and casamino acids present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; and(e) the formulation additionally comprises sorbitol present in a ratio of about 1 to 10 by weight relative to the microbial biomass.

22. The formulation of claim 20 or 21, wherein the maltodextrin has a dextrose equivalent (DE) value of at least 14. WO 2024/168313 PCT/US2024/015273

23. The formulation of claim 1, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass and trehalose present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass.

24. The formulation of claim 23, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.3 to 3 by weight relative to the microbial biomas(c) the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to by weight relative to the microbial biomass and trehalose present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 2.to 29 by weight relative to the microbial biomass.

25. The formulation of claim 23 or 24, wherein the maltodextrin has a dextrose equivalent (DE) value between about 3 and 6.

26. The formulation of claim 1, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; WO 2024/168313 PCT/US2024/015273 (c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass; and(e) the formulation additionally comprises cysteine present in a ratio of about 0.6 to by weight relative to the microbial biomass.

27. The formulation of claim 26, wherein;(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 1.4 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.7 to 7 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 6.2 to by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 2.to 29 by weight relative to the microbial biomass; and(e) the formulation additionally comprises cysteine present in a ratio of about 1.2 to by weight relative to the microbial biomass.

28. The formulation of claim 26 or 27, wherein the maltodextrin has a dextrose equivalent (DE) value between about 6 and 14.

29. The formulation of claim 1 further comprising one or more an oil, a wax, an antioxidant, a pH modifier, a surfactant, an adherent, a bulking agent, a solid diluent, a tackifier, a microbial stabilizer, an antimicrobial, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, a polymer, a protein hydrolysate, a plant extract, rheology modifier, a dispersant, an emulsifier, a colorant, a pH buffer, and/or an anticoagulant.

30. The formulation of claim 1, wherein the weights are wet weights. WO 2024/168313 PCT/US2024/015273

31. The formulation of claim 1, wherein the weights are dry weights.

32. The formulation of claim 1, wherein the microbial biomass comprises a living microorganism, an inactivated microorganism, an attenuated microorganism, a dead microorganism, an extract of a microorganism, and/or a metabolite of a microorganism.

33. The formulation of claim 1, wherein the microbial biomass comprises a living, non-spore forming microorganism.

34. The formulation of claim 1, wherein the microbial biomass comprises a Gram-negative bacteria.

35. The formulation of claim 1, wherein the microbial biomass comprises one or more organism of the phyla/division Pseudomonadota, Bacillota, Actinomycetota, Ascomycota, Basidiomycota, Bacteroidota, Mucoromycota, or Proteobatceria.

36. The formulation of claim 1, wherein the microbial biomass comprises one or more organism of the family Alcaligenaceae, Agaricostdbaceae, Aspergillaceae, Azospirillaceae, Bacdlaceae, Bacteroidaceae, Bifidobacteriaceae, Brevibacteriaceae, Brucellaceae, Burkholderiaceae, C ellulomonadaceae, Chitinophagaceae, Choanephoraceae, Comamonadaceae, Coniochaetaceae, Cystobasidiaceae, Debaryomycetaceae, Dipodascaceae, Enter obacteriaceae, Enterococcaceae, Erwiniaceae, Gymnoascaceae, Helotiaceae, Elypocreaceae, Lactobacillaceae, Meruliaceae, Microascaceae, Microbacteriaceae, Mrakiaceae, Nitrobacteraceae, Ophiocordycipitaceae, Oscillospiraceae, Oxalobacteraceae, Paenibacillaceae, Pichiaceae, Propionibacteriaceae, Pseudomonadaceae, Rhizobiaceae, Rhizopodaceae, Saccharomycetaceae, Sphingomonadaceae, Sirobasidiaceae, Streptococcaceae, Streptomycetaceae, Thermoascaceae, Xanthobacteraceae, Xanthomonadaceae, and/or Xylariaceae. WO 2024/168313 PCT/US2024/015273

37. The formulation of claim 1, wherein the microbial biomass comprises one or more organism comprising at least one polynucleotide sequence that is at least 95% identical to one or more of SEQ ID NOs. 5-57, 114-122, or combinations thereof.

38. The formulation of claim 1, wherein the microbial biomass comprises one or more organism comprising at least one or more genes encoding a protein whose amino acid sequence is selected from the group consisting of SEQ ID NOs. 58-113, or combinations thereof.

39. The formulation of claim 1, wherein the microbial biomass comprises a plurality of microorganisms.

40. The formulation of claim 1, wherein the formulation is a dry solid comprising living microbial biomass present in a concentration of at least 1.0E+03 CFU/g, at least 1.0E+05 CFU/g, at least 1.0E+08 CFU/g, or at least 1.0E+10 CFU/g.

41. The formulation of claim 1, wherein the formulation is a dry solid comprising living microbial biomass present in a concentration between about 1.0E+06 CFU/g and 5.0E+CFU/g.

42. The formulation of claim 1, wherein the formulation is a liquid comprising living microbial biomass present in a concentration of at least 1.0E+03 CFU/mL, at least 1.0E+CFU/mL, at least 1.0E+08 CFU/mL, or at least L0E+10 CFU/mL.

43. The formulation of claim 1, wherein the formulation is a liquid comprising living microbial biomass present in a concentration between about 1.0E+06 CFU/g and 5.0E+CFU/mL.

44. The formulation of claim 1, wherein the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+03 CFU/g in the formulation for at least 30, at least 60, at least 90 days, at least 120 days, at least 150 days, at least 200 days, or at least 250 days. WO 2024/168313 PCT/US2024/015273

45. The formulation of claim 1, wherein the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+03 CFU/g in the formulation at least about 4 degrees C, at least about 22 degrees C, or at least about 35 degrees C.

46. The formulation of claim 1, wherein the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+01 CFU/seed, at least 1.0E+CFU/seed, at least 1.0E+03 CFU/seed, at least 1.0E+04 CFU/seed, or at least 1.0E+05 CFU/seed in the formulation applied to a plant seed for at least 30, at least 60, at least 90 days, at least 1days, at least 150 days, at least 200 days, or at least 250 days.

47. The formulation of claim 1, wherein the formulation comprises living microbial biomass capable of maintaining a viable concentration of at least 1.0E+01 CFU/seed, at least 1.0E+CFU/seed, at least 1.0E+03 CFU/seed, at least 1.0E+04 CFU/seed, or at least 1.0E+05 CFU/seed in the formulation applied to a plant seed when stored at least about 4 degrees C, at least about degrees C, or at least about 35 degrees C.

48. The formulation of claim 1, wherein the formulation comprises living microbial biomass capable of maintaining a viable concentration in the formulation when stored at between about degrees Celsius and 33 degrees Celsius and about between 10% and 50% relative humidity with less than 2 log loss of CFU over 30 or more days.

49. The formulation of claim 48, wherein the formulation is applied to a plant element.

50. The formulation of claim 1, wherein the formulation is a feedstock for spray-drying orlyophilization.

51. The formulation of claim 1, wherein the formulation is a feedstock for an encapsulation technique.

52. The formulation of claim 1, wherein the formulation comprises .3% to 60% total solids. WO 2024/168313 PCT/US2024/015273

53. The formulation of claim L wherein the formulation comprises 20% to 40% total solids

54. The formulation of claim 1, wherein the formulation is a liquid.

55. The formulation of claim 1, wherein the formulation is a solid.

56. A formulation comprising:(a) at least one antioxidant present at a concentration of between 3.5-8.4% by dry weight of the formulation;(b) at least one pH modifier;(c) at least one saccharide present at a concentration of between 4.3-42% by dry weight of the formulation; and(d) at least one protein hydrolysate present at a concentration of between 4-19.1% by dry weight of the formulation.

57. The formulation of claim 56, wherein;(a) the at least one antioxidant comprises ascorbic acid or creatine;(b) the at least one amino acid comprises cysteine or glutathione;(c) the at least one pH modifier comprises sodium bicarbonate, or sodium hydroxide;(d) the at least one saccharide comprises maltodextrin, sucrose, lactose, trehalose, or microcrystalline cellulose; and(e) the at least one protein hydrolysate comprises peptone, tryptone, casamino acids, or a hydrolyzed vegetable protein.

58. The formulation of claim 56, wherein the formulation further comprises at least one inert solid present at a concentration of between 10-32% by dry weight of the formulation.

59. The formulation of claim 58, wherein the at least one inert solid comprises kaolin clay, magnesium stearate, or microcrystalline cellulose.

60. The formulation of claim 56, wherein the at least one saccharide comprises maltodextrin. WO 2024/168313 PCT/US2024/015273

61. The formulation of claim 56, wherein the at least one saccharide comprises maltodextrin and trehalose.

62. The treatment formulation of claim 60, wherein the maltodextrin has a dextrose equivalent (DE) value between 4 and 20.

63. The formulation of claim 56, wherein the formulation further comprises one or more of a sugar alcohol, an amino acid, yeast extract, and/or whey powder.

64. The formulation of claim 56, comprising at least one amino acid present at a concentration of between 2.1-7% by dry weight of the formulation.

65. The formulation of claim 64, wherein the at least one amino acid comprises cysteine or glutathione.

66. A formulation comprising:(a) between about 30-45% by dry weight of maltodextrin;(b) between about 18-19.1% by dry weight of peptone;(c) between about 4.4-5.4% by dry weight of ascorbic acid; and(d) between about 1.6-2.5% by dry weight of sodium bicarbonate.

67. The formulation of claim 66, wherein the formulation further comprises between about 29-32% by dry weight of kaolin clay.

68. The formulation of claim 66, wherein maltodextrin has a dextrose equivalent (DE) of between about 6 and 14.

69. A formulation comprising:(a) between about 30-45% by dry weight of maltodextrin;(b) between about 14-19.1% by dry weight of peptone;(c) between about 3.5-4.5% by dry weight of casamino acids; WO 2024/168313 PCT/US2024/015273 (d) between about 4.5-7% by dry weight of sorbitol;(e) between about 3.2-5.4% by dry weight of ascorbic acid;(f) between about 4.2-7.2% by dry weight of trehalose; and(g) between about 1.6-2.5% by dry weight of sodium bicarbonate.

70. The formulation of claim 69, wherein the formulation further comprises between about 21.2-32% by dry weight of kaolin clay.

71. The formulation of claim 69, wherein maltodextrin has a dextrose equivalent (DE) of at least 14.

72. A formulation comprising:(a) between about 30-45% by dry weight of maltodextrin;(b) between about 16-19.1% by dry weight of peptone;(c) between about 4.4-6% by dry weight of ascorbic acid;(d) between about 4-6% by dry weight of trehalose; and(e) between about 1.6-2.5% by dry weight of sodium bicarbonate.

73. The formulation of claim 72, wherein the formulation further comprises between about 29-32% by dry weight of kaolin clay.

74. The formulation of claim 72, wherein maltodextrin has a dextrose equivalent (DE) of between about 3 and 6.

75. A formulation comprising:(a) between about 30-45% by dry weight of maltodextrin;(b) between about 13.6-19.1% by dry weight of peptone;(c) between about 4.4-10% by dry weight of ascorbic acid;(d) between about 2.1-7.9% by dry weight of sodium bicarbonate; and(e) between about 2.1-5.1% by dry weight of cysteine. WO 2024/168313 PCT/US2024/015273

76. The formulation of claim 75, wherein the formulation further comprises between about 21.2-32% by dry weight of kaolin clay.

77. The formulation of claim 75, wherein maltodextrin has a dextrose equivalent (DE) of between about 6 and 14.

78. The formulation of any one of claims 66, 69, 72, or 75, wherein ascorbic acid is L- ascorbic acid.

79. The formulation of any one of claims 66-78, further comprising one or more an oil, a wax, an antioxidant, a pH modifier, a surfactant, an adherent, a bulking agent, a solid diluent, a tackifier, a microbial stabilizer, an antimicrobial, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, a polymer, a protein hydrolysate, a plant extract, rheology modifier, a dispersant, an emulsifier, a colorant, a pH buffer, and/or an anticoagulant.

80. The formulation of claim 66, 69, 72, or 75, wherein the formulation is applied to a plant element.

81. A method for preparing a synthetic composition, the method comprising heterologously disposing a plurality of plant elements with the formulation of any one of claims 1-79.

82. The method of claim 81, wherein the formulation comprises microbial biomass comprising at least one microorganism, wherein at least one microorganism within the synthetic composition exhibits increased viability compared to a reference microorganism.

83. The method of claim 81, wherein the at least one microorganism within the synthetic composition retains viability for at least 50 days, at least 100 days, at least 150 days, at least 2days, at least 250 days, at least 300 days, at least 400 days, or at least 500 days when stored at degrees Celsius. WO 2024/168313 PCT/US2024/015273

84. The method of claim 81, wherein the at least one microorganism within the synthetic composition retains viability for at least 50 days, at least 100 days, at least 150 days, at least 2days, at least 250 days, at least 300 days, at least 400 days, or at least 500 days when stored at degrees Celsius.

85. The method of claim 81, wherein the time to 1 log loss in CPU of the at least one microorganism in synthetic composition is at greater than or equal to 168 days, greater than or equal to 150 days, greater than or equal to 125 days, greater than or equal to 100 days, greater than or equal to 75 days, greater than or equal to 50 days, greater than or equal to 20 days, at degrees Celsius.

86. The method of claim 81, wherein the time to 2 log loss in CPU of the at least one microorganism on a seed is at least 3 days, at least 5 days, at least 10 days, at least 20 days, at least 30 days, at least 60 days, at least 100 days, at least 200 days, at 22 degrees Celsius.

87. A method of improving plant health comprising heterologously disposing an effective amount of one or more microorganisms to a plant element to increase a trait of agronomic importance in the plant derived from the treated plant element relative to a plant derived from a reference plant element,wherein the one or more microorganisms are comprised within the formulation of any one of claims 1-79.

88. The method of claim 87, wherein the treated plant exhibits an improvement in tolerance to a stress condition relative to a plant derived from a reference plant element.

89. The method of claim 88, wherein the stress condition is an abiotic stress selected from the group consisting of: drought stress, salt stress, metal stress, heat stress, cold stress, low nutrient stress, and excess water stress, and combinations thereof

90. The method of claim 88, wherein the stress condition is a biotic stress selected from the group consisting of: insect infestation, nematode infestation, complex infection, fungal infection, WO 2024/168313 PCT/US2024/015273 bacterial infection, oomycete infection, protozoal infection, viral infection, herbivore grazing, and combinations thereof

91. The method of any one of claims 88-90, wherein an improvement in stress tolerance is evaluated by improvement of one or more other traits of agronomic importance when compared with a reference plant, reference plant element, or reference population.

92. The method of claim 90 or 91, wherein an improvement in biotic stress tolerance is evaluated by one or more of: decreased pathogen load of tissues, decreased area of chlorotic tissue, decreased necrosis, improved growth, increased survival, increased biomass, increased shoot height, increased root length, or combinations thereof relative to a reference plant derived from a reference plant element.

93. A method for producing a spray dried formulation comprising:(a) preparing a feedstock comprising microbial biomass; one or more antioxidant in a ratio of about 0.07 to 14 by weight relative to the microbial biomass; one or more pH modifier in a ratio of about 0.03 to 7 by weight relative to the microbial biomass; one or more saccharide in a ratio of about 0.07 to 62 by weight relative to the microbial biomass; and one or more protein hydrolysate in a ratio of about 0.07 to 29 by weight relative to the microbial biomass, the microbial biomass have a first concentration;(b) spray drying the feedstock, wherein spray drying comprises introducing the feedstock into the spray dryer using an inlet temperature of between about 75 degrees Celsius and 140 degrees Celsius and an outlet temperature of between about 45 degrees Celsius and 65 degrees Celsius, to generate an emitted spray dried formulation having a second concentration.

94. The method of claim 93, wherein the feedstock comprises one or more antioxidant in a ratio of at least about 0.7 by weight relative to the microbial biomass; one or more pH modifier in a ratio of at least about 0.3 by weight relative to the microbial biomass; one or more saccharide in a ratio of at least about 1 by weight relative to the microbial biomass; and one or more protein hydrolysate in a ratio of at least about 0.7 by weight. WO 2024/168313 PCT/US2024/015273

95. The method of claim 93, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone in a ratio of about 1.45 to by weight relative to the microbial biomass.

96. The method of claim 93, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass and trehalose present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass;(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.to 24 by weight relative to the microbial biomass and casamino acids present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and(e) the formulation additionally comprises sorbitol present in a ratio of about 0.5 to 10 by weight relative to the microbial biomass.

97. The method of claim 93, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.35 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.15 to 3 by weight relative to the microbial biomass; 100 WO 2024/168313 PCT/US2024/015273 (c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass and trehalose present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass; and(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass.

98. The method of claim 93, wherein:(a) the one or more antioxidant comprises ascorbic acid present in a ratio of about 0.7 to by weight relative to the microbial biomass;(b) the one or more pH modifier comprises sodium bicarbonate present in a ratio of about 0.35 to 7 by weight relative to the microbial biomass;(c) the one or more saccharide comprises maltodextrin present in a ratio of about 3.1 to by weight relative to the microbial biomass;(d) the one or more protein hydrolysate comprises peptone present in a ratio of about 1.45 to 29 by weight relative to the microbial biomass; and(e) the formulation additionally comprises cysteine present in a ratio of about 0.6 to by weight relative to the microbial biomass.

99. The method of claim 93, wherein the second concentration is at least 20%, at least 40%, or at least 75% of the first concentration.

100. The method of claim 93, wherein the second concentration is at least 1.0E+03 CFU/g, at least 1.0E+05 CFU/g, or at least 1.0E+08 CFU/g.

101. The method of claim 93, wherein the at least one microorganism within the spray dried formulation composition retains viability for at least 30 days, 50 days, at least 100 days, at least 150 days, at least 200 days, at least 250 days, at least 300 days, at least 400 days, or at least 5days when stored at 4 degrees Celsius, as measured by a less than one log loss in the second concentration over that time. 101 WO 2024/168313 PCT/US2024/015273

102. The method of claim 93, wherein the at least one microorganism within the spray dried formulation composition retains viability for at least 30 days, 50 days, at least 100 days, at least 150 days, at least 200 days, at least 250 days, at least 300 days, at least 400 days, or at least 5days when stored at 22 degrees Celsius, as measured by a less than one log loss in the second concentration over that time. 102