IL321197A - Nanobody-functionalized biological nanopores and means and methods related thereto - Google Patents

Nanobody-functionalized biological nanopores and means and methods related thereto

Info

Publication number
IL321197A
IL321197A IL321197A IL32119725A IL321197A IL 321197 A IL321197 A IL 321197A IL 321197 A IL321197 A IL 321197A IL 32119725 A IL32119725 A IL 32119725A IL 321197 A IL321197 A IL 321197A
Authority
IL
Israel
Prior art keywords
nanobody
methods related
biological nanopores
functionalized biological
functionalized
Prior art date
Application number
IL321197A
Other languages
Hebrew (he)
Inventor
Giovanni Maglia
Xialin Zhang
J?rgen KJEMS
Original Assignee
Univ Groningen
Univ Aarhus
Giovanni Maglia
Xialin Zhang
J?rgen KJEMS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Groningen, Univ Aarhus, Giovanni Maglia, Xialin Zhang, J?rgen KJEMS filed Critical Univ Groningen
Publication of IL321197A publication Critical patent/IL321197A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/197Modifications characterised by incorporating a spacer/coupling moiety
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
IL321197A 2022-12-02 2025-05-29 Nanobody-functionalized biological nanopores and means and methods related thereto IL321197A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP22211193 2022-12-02
PCT/NL2023/050633 WO2024117910A1 (en) 2022-12-02 2023-12-01 Nanobody-functionalized biological nanopores and means and methods related thereto

Publications (1)

Publication Number Publication Date
IL321197A true IL321197A (en) 2025-07-01

Family

ID=84387796

Family Applications (1)

Application Number Title Priority Date Filing Date
IL321197A IL321197A (en) 2022-12-02 2025-05-29 Nanobody-functionalized biological nanopores and means and methods related thereto

Country Status (6)

Country Link
EP (1) EP4627343A1 (en)
JP (1) JP2026500150A (en)
CN (1) CN120500624A (en)
AU (1) AU2023400581A1 (en)
IL (1) IL321197A (en)
WO (1) WO2024117910A1 (en)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
EP2383776B1 (en) 1999-06-22 2015-02-25 President and Fellows of Harvard College Solid state nanopore device for evaluating biopolymers
US6464842B1 (en) 1999-06-22 2002-10-15 President And Fellows Of Harvard College Control of solid state dimensional features
EP1212462B1 (en) 1999-09-07 2006-01-25 The Regents of the University of California Methods of determining the presence of double stranded nucleic acids in a sample
US20030104428A1 (en) 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
US6870361B2 (en) 2002-12-21 2005-03-22 Agilent Technologies, Inc. System with nano-scale conductor and nano-opening
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
KR20200119291A (en) * 2018-02-15 2020-10-19 에프. 호프만-라 로슈 아게 Nanopore protein conjugate for detection and analysis of analytes
WO2022140655A1 (en) * 2020-12-23 2022-06-30 The Trustees Of Columbia University In The City Of New York Single-molecule electronic multiplex nanopore immunoassays for biomarker detection

Also Published As

Publication number Publication date
EP4627343A1 (en) 2025-10-08
AU2023400581A1 (en) 2025-06-19
CN120500624A (en) 2025-08-15
JP2026500150A (en) 2026-01-06
WO2024117910A1 (en) 2024-06-06

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