IL309867A - שיטות להכנת ספריות לריצוף דנ" א - Google Patents

שיטות להכנת ספריות לריצוף דנ" א

Info

Publication number
IL309867A
IL309867A IL309867A IL30986723A IL309867A IL 309867 A IL309867 A IL 309867A IL 309867 A IL309867 A IL 309867A IL 30986723 A IL30986723 A IL 30986723A IL 309867 A IL309867 A IL 309867A
Authority
IL
Israel
Prior art keywords
dna
methylation
sequencing
digestion
restriction
Prior art date
Application number
IL309867A
Other languages
English (en)
Inventor
Knirsh Revital
Edward Dodge Michael
Bram Eran
Ezhevsky Sergei
Original Assignee
Nucleix Ltd
Knirsh Revital
Edward Dodge Michael
Bram Eran
Ezhevsky Sergei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nucleix Ltd, Knirsh Revital, Edward Dodge Michael, Bram Eran, Ezhevsky Sergei filed Critical Nucleix Ltd
Priority to IL309867A priority Critical patent/IL309867A/he
Priority to PCT/IL2024/051230 priority patent/WO2025141580A1/en
Publication of IL309867A publication Critical patent/IL309867A/he

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
IL309867A 2023-12-31 2023-12-31 שיטות להכנת ספריות לריצוף דנ" א IL309867A (he)

Priority Applications (2)

Application Number Priority Date Filing Date Title
IL309867A IL309867A (he) 2023-12-31 2023-12-31 שיטות להכנת ספריות לריצוף דנ" א
PCT/IL2024/051230 WO2025141580A1 (en) 2023-12-31 2024-12-29 Methods for preparing dna sequencing libraries

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IL309867A IL309867A (he) 2023-12-31 2023-12-31 שיטות להכנת ספריות לריצוף דנ" א

Publications (1)

Publication Number Publication Date
IL309867A true IL309867A (he) 2025-07-01

Family

ID=96217426

Family Applications (1)

Application Number Title Priority Date Filing Date
IL309867A IL309867A (he) 2023-12-31 2023-12-31 שיטות להכנת ספריות לריצוף דנ" א

Country Status (2)

Country Link
IL (1) IL309867A (he)
WO (1) WO2025141580A1 (he)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022107145A1 (en) * 2020-11-19 2022-05-27 Nucleix Ltd. Detecting methylation changes in dna samples using restriction enzymes and high throughput sequencing
WO2023283591A2 (en) * 2021-07-07 2023-01-12 The Regents Of The University Of California Methods of methylation analysis for disease detection
WO2023227954A1 (en) * 2022-05-22 2023-11-30 Nucleix Ltd. Sample preparation for cell-free dna analysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022107145A1 (en) * 2020-11-19 2022-05-27 Nucleix Ltd. Detecting methylation changes in dna samples using restriction enzymes and high throughput sequencing
WO2023283591A2 (en) * 2021-07-07 2023-01-12 The Regents Of The University Of California Methods of methylation analysis for disease detection
WO2023227954A1 (en) * 2022-05-22 2023-11-30 Nucleix Ltd. Sample preparation for cell-free dna analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FENG, L., LOU, J., DNA METHYLATION ANALYSIS., 14 December 2018 (2018-12-14) *
LI, DAOFENG, ET AL., COMBINING MEDIP-SEQ AND MRE-SEQ TO INVESTIGATE GENOME-WIDE CPG METHYLATION., 15 January 2015 (2015-01-15) *

Also Published As

Publication number Publication date
WO2025141580A1 (en) 2025-07-03

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