IL303588A - Protein substrate to bind growth factor - Google Patents
Protein substrate to bind growth factorInfo
- Publication number
- IL303588A IL303588A IL303588A IL30358823A IL303588A IL 303588 A IL303588 A IL 303588A IL 303588 A IL303588 A IL 303588A IL 30358823 A IL30358823 A IL 30358823A IL 303588 A IL303588 A IL 303588A
- Authority
- IL
- Israel
- Prior art keywords
- seq
- binding motif
- protein substrate
- heparin binding
- protein
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title claims 23
- 108090000623 proteins and genes Proteins 0.000 title claims 23
- 239000000758 substrate Substances 0.000 title claims 19
- 239000003102 growth factor Substances 0.000 title claims 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims 17
- 229960002897 heparin Drugs 0.000 claims 17
- 229920000669 heparin Polymers 0.000 claims 17
- 108010044426 integrins Proteins 0.000 claims 9
- 102000006495 integrins Human genes 0.000 claims 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims 5
- 239000000853 adhesive Substances 0.000 claims 4
- 230000001070 adhesive effect Effects 0.000 claims 4
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 3
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 3
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 3
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 3
- 230000003213 activating effect Effects 0.000 claims 3
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 3
- 108010035532 Collagen Proteins 0.000 claims 2
- 102000008186 Collagen Human genes 0.000 claims 2
- 108010067306 Fibronectins Proteins 0.000 claims 2
- 102000016359 Fibronectins Human genes 0.000 claims 2
- 108010028750 Integrin-Binding Sialoprotein Proteins 0.000 claims 2
- 102000016921 Integrin-Binding Sialoprotein Human genes 0.000 claims 2
- 108010085895 Laminin Proteins 0.000 claims 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 claims 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 claims 2
- 108010031318 Vitronectin Proteins 0.000 claims 2
- 102100035140 Vitronectin Human genes 0.000 claims 2
- 210000004899 c-terminal region Anatomy 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 229920001436 collagen Polymers 0.000 claims 2
- 108010004563 mussel adhesive protein Proteins 0.000 claims 2
- 239000003988 mussel adhesive protein Substances 0.000 claims 2
- 108010069848 AG 73 Proteins 0.000 claims 1
- 108010009202 Growth Factor Receptors Proteins 0.000 claims 1
- 102000009465 Growth Factor Receptors Human genes 0.000 claims 1
- 108010042918 Integrin alpha5beta1 Proteins 0.000 claims 1
- 108010033113 collagen type IV alpha1 (531-543) Proteins 0.000 claims 1
- 230000007705 epithelial mesenchymal transition Effects 0.000 claims 1
- LZLBNEPMZQVGQQ-FCDAVOGYSA-N gefyfdlrlkgdk Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCC(O)=O)NC(=O)CN)C1=CC=CC=C1 LZLBNEPMZQVGQQ-FCDAVOGYSA-N 0.000 claims 1
- 108010068609 integrin alpha 9 beta 1 Proteins 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 claims 1
- 230000011664 signaling Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Claims (19)
1.[Claim 1] A protein substrate comprising a recombinant adhesive protein genetically functionalized with an integrin binding motif and a heparin binding motif which is capable of binding or sequestering growth factors.
2.[Claim 2] The protein substrate of claim 1, wherein said heparin binding motif is derived from the group consisting of fibronectin domain III, laminin globular domain, heparin binding domain of collagen, vitronectin, and bone sialoprotein.
3.[Claim 3] The protein substrate of claim 2, wherein said heparin binding motif derived from fibronectin domain III is selected from the group consisting of KYILRWRPKNS (SEQ ID NO: 7), YRVRVTPKEKTGPMKE (SEQ ID NO: 8), SPPRRARVT (SEQ ID NO: 9), ATETTITIS (SEQ ID NO: 10), VSPPRRARVTDATETTITISWRTKTETITGFG (SEQ ID NO: 11), ANGQTPIQRYIK (SEQ ID NO: 12), KPDVRSYTITG (SEQ ID NO: 13), PRARITGYIIKYEKPGSPPREVVPRPRPGV (SEQ ID NO: 14), WQPPRARI (SEQ ID NO: 15), WQPPRARITGYIIKYEKPG (SEQ ID NO: 16), YEKPGSPPREVVPRPRP (SEQ ID NO: 17), and KNNQKSEPLIGRKKT (SEQ ID NO: 18).
4.[Claim 4] The protein substrate of claim 2, wherein said heparin binding motif derived from laminin globular domain is selected from the group consisting of GLIYYVAHQNQM (SEQ ID NO: 19), RKRLQVQLSIRT (SEQ ID NO: 20), GLLFYMARINHA (SEQ ID NO: 21), KNSFMALYLSKG (SEQ ID NO: 22), VVRDITRRGKPG (SEQ ID NO: 23), RAYFNGQSFIAS (SEQ ID NO: 24), GEKSQFSIRLKT (SEQ ID NO: 25), TLFLAHGRLVFMFNVGHKKL (SEQ ID NO: 26), TLFLAHGRLVFM (SEQ ID NO: 27), LVFMFNVGHKKL (SEQ ID NO: 28), GAAWKIKGPIYL (SEQ ID NO: 29), VIRDSNVVQLDV (SEQ ID NO: 30), GKNTGDHFVLYM (SEQ ID NO: 31), RLVSYSGVLFFLK (SEQ ID NO: 32), GPLPSYLQFVGI (SEQ ID NO: 33), RNRLHLSMLVRP (SEQ ID NO: 34), LVLFLNHGHFVA (SEQ ID NO: 35), AGQWHRVSVRWG (SEQ ID NO: 36), KMPYVSLELEMR (SEQ ID NO: 37), RYVVLPR (SEQ ID NO: 38), VRWGMQQIQLVV (SEQ ID NO: 39), TVFSVDQDNMLE (SEQ ID NO: 40), APMSGRSPSLVLK (SEQ ID NO: 41), VLVRVERATVFS (SEQ ID NO: 42), PGRWHKVSVRWE (SEQ ID NO: 76) and RNIAEIIKDI (SEQ ID NO: 43).
5.[Claim 5] The protein substrate of claim 2, wherein said heparin binding motif derived from heparin binding domain of collagen is selected from the group consisting of KGHRGF (SEQ ID NO: 44), TAGSCLRKFSTM (SEQ ID NO: 45), and GEFYFDLRLKGDK (SEQ ID NO: 46).
6.[Claim 6] The protein substrate of claim 2, wherein said heparin binding motif derived from heparin binding domain of vitronectin is KKQRFRHRNRKGYRSQ (SEQ ID NO: 47).
7.[Claim 7] The protein substrate of claim 2, wherein said heparin binding motif derived from heparin binding domain of bone sialoprotein is KRSR (SEQ ID NO: 48), or KRRA (SEQ ID NO: 49).
8.[Claim 8] The protein substrate of claim 1, wherein said heparin binding motif is capable of binding basic fibroblast growth factor (bFGF), transforming growth factor β (TGF-β), or platelet derived growth factor (PDGF).
9.[Claim 9] The protein substrate of claim 1, wherein said integrin binding motif is selected from αvβ3-, αvβ6-, αvβ8-, α5β1-, α9β1 binding peptide.
10.[Claim 10] The protein substrate of claim 1, wherein said integrin binding motif is capable of activating integrin αvβ6 and said heparin binding motif is capable of binding TGF-β.
11.[Claim 11] The protein substrate of claim 1, wherein said integrin binding motif is capable of activating integrin α5β1 or α9β1 and said heparin binding motif is capable of binding bFGF.
12.[Claim 12] The protein substrate of claim 1, wherein the recombinant adhesive protein is derived from a recombinant mussel adhesive protein.
13.[Claim 13] The protein substrate of claim 1, wherein the recombinant mussel adhesive protein comprises the peptide selected from the group consisting of SEQ ID Nos: 1-6, and 60-74.
14.[Claim 14] The protein substrate of claim 1, wherein the integrin binding motif and/or the heparin binding motif is bound to N-terminal and/or C-terminal of the recombinant adhesive protein.
15.[Claim 15] The protein substrate of claim 1, wherein both of the integrin binding motif and the heparin binding motif are bound to N-terminal or C-terminal of the recombinant adhesive protein.
16.[Claim 16] The protein substrate of claim 15, wherein the integrin binding motif and the heparin binding motif is connected via a spacer linker peptide.
17.[Claim 17] The protein substrate of claim 16, wherein the spacer linker peptide is a peptide of SEQ ID NO: 75.
18.[Claim 18] An extracellular microenvironment surface to regulate cell plasticity, wherein said microenvironment surface comprises the protein substrate of any one of claims 1 to 17 that can induce combinatorial signaling via activating simultaneously integrins and growth factor receptors.
19.[Claim 19] The extracellular microenvironment surface of claim 18, wherein said cell plasticity is epithelial-mesenchymal transition.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202016126245A | 2020-12-16 | 2020-12-16 | |
| PCT/KR2021/019159 WO2022131809A1 (en) | 2020-12-16 | 2021-12-16 | Protein substrate to bind growth factor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL303588A true IL303588A (en) | 2023-08-01 |
Family
ID=87517062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL303588A IL303588A (en) | 2020-12-16 | 2021-12-16 | Protein substrate to bind growth factor |
Country Status (1)
| Country | Link |
|---|---|
| IL (1) | IL303588A (en) |
-
2021
- 2021-12-16 IL IL303588A patent/IL303588A/en unknown
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