IL297988A - Antibodies against sars-cov-2 - Google Patents

Description

WO 2021/226560 PCT/US2021/031442 ANTIBODIES AGAINST SARS-COV-2 STATEMENT REGARDING SEQUENCE LISTING The Sequence Listing associated with this application is provided in text format in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is 930585_409WO_SEQUENCE_LISTING.txt. The text file is 489 KB, was created on May 7, 2021, and is being submitted electronically via EFS-Web.

BACKGROUND A novel betacoronavirus emerged in Wuhan, China, in late 2019. As of May 2, 2021, approximately 152 million cases of infection by this virus (termed, among other names, SARS-C0V-2 and Wuhan coronavirus) were confirmed worldwide, and had resulted in approximately 3.195 million deaths. Therapies for preventing or treating SARS-C0V-2 infection are needed.

BRIEF DESCRIPTION OF THE DRAWINGS Figures 1A-1Dshow binding of certain antibodies to SARS-C0V-2 Spike protein RBD and SARS-C0V-1 Spike protein RBD. Human monoclonal antibodies isolated from patients who recovered from SARS-C0V-2 infection were recombinantly expressed and tested for RBD binding by ELISA. Figure 1A shows binding of five antibodies, Figure IB and Figure IC each show binding of seven antibodies, and Figure ID shows binding of four antibodies. Two antibodies in the top panel of Figure IB are shown using black symbols. In Figure IB, S2X28 binding is represented by a line that stays at or near zero OD for the full range of concentrations tested. In Figure IB (top), S2X41 binding is represented by the curve with an EC50 of approximarely 20.01 ng/ml. In each of Figures 1A-1D, the top panel shows binding to SARS-C0V-2 RBD and the bottom panel shows binding to SARS-C0V-1 RBD. The boxes on the right side of each figure, where present, indicate the calculated EC50 value for the indicated antibody.

WO 2021/226560 PCT/US2021/031442 Figure 2shows inhibition of SARS-CoV-2 RBD binding to human ACE2 by recombinantly expressed monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection. ELISA plates were coated with recombinant human ACE2 (produced in-house). Coating was carried out with ACE2 at 2ug/ml in PBS. Plates were incubated overnight at 4°C and blocking was performed with blocker Casein (1% Casein from Thermofisher) for 1 hour at room temperature. Figures 3A-3Fshow results from neutralization of infection assays using certain antibodies against SARS-CoV-2 pseudotyped virus. Monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested in neutralization assays against murine leukemia virus (MEV) pseudotyped with SARS-CoV-2 Spike protein. Figure 3 A shows results for three antibodies. Figures 3B-3F each show results for four antibodies. Antibodies were tested at concentrations indicated in the x-axis. Boxes on the right side of each figure, where present, indicate the calculated IC50 for the indicated antibody. Figures 4A-4Nshow binding of further antibodies to SARS-CoV-2 Spike protein, SARS-CoV-2 Spike protein RBD, and SARS-C0V-1 Spike protein RBD. Monoclonal antibodies isolated from patients who recovered from SARS-CoV-infection were expressed recombinantly and tested for RBD binding by ELISA. The boxes on the right side of each figure show the calculated EC50 value for the indicated antibody. Figures 5A-5Dshow results from neutralization of infection assays using further antibodies against SARS-CoV-2 pseudotyped virus. Monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested in neutralization assays (one antibody per assay) against murine leukemia virus (MLV) pseudotyped with SARS-CoV-2 Spike protein. Figures A and 5C each show results for three antibodies. Figure 5B shows results for two antibodies and Figure 5D shows results for four antibodies. Antibodies were tested at concentrations as indicated on the x-axis. Boxes on the right side of each figure, where present, show the calculated EC50 for the indicated antibody.

WO 2021/226560 PCT/US2021/031442 Figures 6A and 6Bshow results from neutralization of infection assays using monoclonal antibodies against authentic SARS-CoV-2 virus. Comparator antibody "S309-v2" comprises the VH amino acid sequence set forth in SEQ ID NO.: 342 and the VL amino acid sequence set forth in SEQ ID NO.: 346 (CDRH1-H3 and L1-L3 as set forth in SEQ ID NOs. :343-345 and 347-349, respectively), and is an engineered variant of an antibody isolated from a patient who recovered from SARS-CoV-infection. Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and lx Penicillin/Streptomycin (Thermo Fisher Scientific) were seeded in white 96-well plates at 20,000 cells/well and attached overnight. Serial 1:4 dilutions of the antibodies were incubated with 200 pfu of SARS-CoV-2 (isolate USA-WA1/2020, passage 3, passaged in Vero E6 cells) for 30 minutes at 37°C in a BSL-3 facility. Cell supernatant was removed and the virus-antibody mixture was added to the cells. 24 hours post infection, cells were fixed with 4% paraformaldehyde for 30 minutes, followed by two PBS (pH 7.4) washes and permeabilization with 0.25% Triton X-100 in PBS for minutes. After blocking in 5% milk powder/PBS for 30 minutes, cells were incubated with a primary antibody targeting SARS-CoV-2 nucleocapsid protein (Sino Biological, cat. 40143-R001) at a 1:2000 dilution for !hour. After washing and incubation with a secondary Alexa647-labeled antibody mixed with 1 ug/ml Hoechst33342 for 1 hour, plates were imaged on an automated cell-imaging reader (Cytation 5, Biotek) and nucleocapsid-positive cells were counted using the manufacturer ’s supplied software. Data were processed using Prism software (GraphPad Prism 8.0). Figures 7A-7Dshow results from neutralization of infection assays using further antibodies against SARS-CoV-2 pseudotyped virus. Monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested in neutralization assays against murine leukemia virus (MEV) pseudotyped with SARS-CoV-2 Spike protein. Figures 7A and 7B each show results for four antibodies, along with comparator antibody S309 (VH amino acid sequence of SEQ ID NO.:139, VL amino acid sequence of SEQ ID NO.:143; CDRH1-H3 and El- L3 as set forth in SEQ ID NOs. :140-142 and 144-146, respectively), isolated from a patient who recovered from SARS-CoV-1 infection. Figure 7C shows results for three WO 2021/226560 PCT/US2021/031442 antibodies along with S309, and Figure 7D shows results for two antibodies along with S309. Antibody "S2H58" in Figure 7D comprises the VH amino acid sequence set forth in SEQ ID NO.: 228 and the VL amino acid sequence set forth in SEQ ID NO.: 238. Antibodies were tested at concentrations as indicated on the x-axis. Figures 8A and 8Bshow binding of antibodies to SARS-CoV-2 Spike protein RED and SARS-CoV-1 Spike protein RED. Monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested for RED binding by ELISA. Figure 8A shows binding of five antibodies and one comparator antibody, S309 (VH amino acid sequence of SEQ ID NO. :139, VL amino acid sequence of SEQ ID NO.: 143; CDRH1-H3 and L1-L3 as set forth in SEQ ID NOs.: 140-142 and 144-146, respectively), isolated from a patient who recovered from SARS-CoV-1 infection. Figure 8B shows binding of four antibodies and S309. In each of Figures 8 A and 8B, the left panel shows binding to SARS-CoV-2 RED and the right panel shows binding to SARS-CoV-1 RED. The boxes on the right side of each panel indicate the calculated EC50 value for the indicated antibody. Figures 9A-9Fshow results from neutralization of infection assays using antibodies against SARS-CoV-2 pseudotyped virus. Monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested in neutralization assays against murine leukemia virus (MLV) pseudotyped with SARS-CoV-2 Spike protein. Antibodies were tested at concentrations as indicated on the x-axis. Calculated IC50, IC80, and IC90 values (expressed as ng/ml) are shown below the graph in each figure. Figures 10A-10Eshow binding of antibodies to SARS-CoV-2 Spike protein RED and SARS-CoV-1 Spike protein RED (SARS-CoV-1 Spike protein RED being labeled "SARS RED" in the lower graph in each figure). Monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested for RED binding by ELISA. In each of Figures 10A-10E, the top panel shows binding to SARS-CoV-2 RED and the bottom panel shows binding to SARS-CoV-1 RED. The boxes on the right side of each figure, where present, show the calculated EC50 value for the indicated antibody.

WO 2021/226560 PCT/US2021/031442 Figures 11A-11Dshow results from neutralization of infection assays using certain monoclonal antibodies. Antibodies were tested in neutralization assays against murine leukemia virus (MEV) pseudotyped with SARS-CoV-2 Spike protein. Figure 11A shows results for monoclonal antibodies S2X193 and S2X195. Figure 1 IB shows results for monoclonal antibodies S2X219 and S2X244. Figure 1 IC shows results for monoclonal antibodies S2X246 and S2X256. Figure 1 ID shows results for monoclonal antibody S2X278. The x-axis shows the total concentration of antibody. Calculated IC50, IC80, and IC90 values (expressed as ng/ml) are shown in the box on the right side of each figure. Figures 12A-12Dshow results from neutralization of infection assays using certain monoclonal antibodies. Antibodies were expressed recombinantly and tested in neutralization assays against vesicular stomatitis virus (VSV) pseudotyped with SARS- C0V-2 Spike protein. Figure 12A shows results for monoclonal antibodies S2X193 and S2X195, along with comparator antibody S2X190. Figure 12B shows results for monoclonal antibody S2X219. Figure 12C shows results for monoclonal antibodies S2X244, S2X246, and S2X256. Figure 12D shows results for monoclonal antibodies S2X269 and S2X278. Antibodies were tested at concentrations indicated on the x-axis. Calculated IC50 and IC90 values (expressed as ng/ml) are shown at the bottom of each figure. Figures 13A and 13Bshow the ability of certain monoclonal antibodies to inhibit binding by SARS-CoV-2 RBD to human ACE2. ELISA plates were coated with recombinant human ACE2 at 2 ug/ml in PBS. Serial dilutions of monoclonal antibodies were incubated with SARS-CoV-2 RBD at 20 ng/ml (RBD fused with mouse Fc, from Sino Biological) for 30 minutes at 37°C and then transferred onto the ACE2- coated plates for an additional 20 minute incubation at room temperature. Eleven serial dilutions were used, starting at 10 ug/ml and diluting at 1:3. Binding of RBD to ACEwas detected using secondary antibody goat F(ab ’)2 anti-mouse IgG(H+L) antibody (Southern Biotech) conjugated to alkaline phosphatase, followed by addition of pNPP (Sigma Aldrich N2765-100TAB) in bicarbonate buffer and reading absorbance at 405nm.

WO 2021/226560 PCT/US2021/031442 Figure 13 A shows results for monoclonal antibodies S2X193 and S2X195, along with four comparator antibodies. Figure 13B shows results for monoclonal antibodies S2X219, S2X244, S2X246, S2X256, S2X269, and S2X278. Calculated IC50 values are shown at or to the right of the graph in each figure. Figures 14A-14Hshow binding affinity and avidity of certain monoclonal antibodies of the present disclosure to SARS-CoV-2 RBD, as measured by Octet. Antibody (as indicated at the bottom-right of each figure) was loaded on Protein A pins at 2.7 ug/ml. SARS-CoV-2 RBD was loaded for 5 minutes at 6 ug/ml, 1.5 ug/ml, or 0.4 ug/ml. Dissociation was measured for 7 minutes. The vertical dashed line in each figure indicates the start of the dissociation phase. Figure 15shows binding affinity and avidity of monoclonal antibodies S2X2(first panel) and S2X193, S2X195, S2X244, S2X246, S2X256, S2X269, and S2X278, along with four comparator antibodies (second panel), to SARS-CoV-1 RBD as measured by Octet. Antibody was loaded on Protein A pins at 2.7 ug/ml. SARS-C0V- RBD was loaded for 5 minutes at 6 ug/ml. Dissociation was measured for 7 minutes. The vertical dashed line in each graph indicates the start of the dissociation phase. In the second panel, the order of the curves curves (top to bottom) corresponds to the antibodies listed to the right of the graph (top to bottom). By way of illustration, the top curve corresponds to S2X127 and the bottom curve corresponds to S2X278. Figures 16A-16Dshow neutralization of SARS-CoV-2 infection by certain monoclonal antibodies, as assessed by inhibition of nucleocapsid (NP) expression at hours post infection. Figure 16A shows neutralization of SARS-CoV-2 infection by monoclonal antibodies S2N22, S2N12, S2N28, S2N25, S2H58-v2, along with comparator antibody S309-v2 (VH amino acid sequence of SEQ ID NO.:342, VL amino acid sequence of SEQ ID NO. :346; CDRH1-H3 and L1-L3 as set forth in SEQ ID NOs. :343-345 and 347-349, respectively). Figure 16B shows neutralization of SARS-CoV-2 infection by monoclonal antibodies S2E9, S2E6, S2E13, S2K4, S2E14, S2E7, and S2E12 (VH amino acid sequence of SEQ ID NO. :399, VL amino acid sequence of SEQ ID NO.:403; CDRH1-H3 and L1-L3 as set forth in SEQ ID NOs.:400, 766, 402 and 404-406, respectively), along with comparator antibody S309-v2. Figure WO 2021/226560 PCT/US2021/031442 16C shows neutralization of SARS-CoV-2 infection by monoclonal antibodies S2H37, S2H73, S2H40, S2H70, and S2H71, along with comparator antibody S309-v2 (with M428L/N434S Fc mutations). Figure 16D shows neutralization of SARS-CoV-infection by monoclonal antibodies S2X30, S2H58-vl, S2H66, S2H62, and S2H30, along with comparator antibody S309-v2. Calculated IC50 values are shown below each graph. Figures 17A-17Cshow results from neutralization of infection assays using certain monoclonal antibodies. Figure 17A shows results for monoclonal antibodies S2M11 and S2M28. Figure 17B shows results for monoclonal antibody S2M16. Figure 17C shows results for monoclonal antibodies S2M7 and S2L49. Antibodies were tested in neutralization assays against murine leukemia virus (MLV) pseudotyped with SARS-CoV-2 Spike protein. The x-axis shows the total concentration of antibody. Calculated IC50, IC80, and IC90 values (expressed as ng/ml) are shown in the box below each graph. Figures 18A-18Eshow binding of certain monoclonal antibodies to SARS- C0V-2 Spike protein, SARS-CoV-2 Spike protein RBD, SARS-CoV-1 Spike protein, and SARS-CoV-1 Spike protein RBD. Antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested for Spike and Spike RBD binding by ELISA. The boxes on the right side of each figure show calculated EC50 values (expressed as ng/ml). Figures 19A-19Eshow results from neutralization of infection assays using certain monoclonal antibodies. Antibodies were tested in neutralization assays against murine leukemia virus (MLV) pseudotyped with SARS-CoV-2 Spike protein. Figure 19A shows results for monoclonal antibodies S2X149 and S2X179. Figure 19B shows results for monoclonal antibody S2D65. Figure 19C shows results for monoclonal antibody S2D97. Figure 19D shows results for monoclonal antibody S2D106. Figure 19E shows results for monoclonal antibody S2H101. The x-axis shows the total concentration of antibody. Calculated IC50, IC80, and IC90 values are shown in the box on the right side of each figure, in the left-hand column of the box.

WO 2021/226560 PCT/US2021/031442 Figures 20A and 20Bshow binding of human monoclonal antibody S2X1and comparator antibody S309-v2 LS (VH amino acid sequence of SEQ ID NO.:342, VL amino acid sequence of SEQ ID NO. :346; CDRH1-H3 and L1-L3 as set forth in SEQ ID NOs. :343-345 and 347-349, respectively; expressed as rIgGI with M428L and N434S Fc mutations) to SARS-CoV-1 Spike protein, SARS-CoV-1 Spike protein RED, and SARS-C0V-2 Spike protein RED. Human monoclonal antibodies were expressed recombinantly and binding was tested by ELISA. Figure 20A shows binding of antibodies to SARS-CoV-1 Spike protein RED (top panel) and SARS-CoV-1 Spike protein (bottom panel). Figure 20B shows binding of antibodies to SARS-C0V-2 Spike protein RED (top panel) and to an uncoated control plate (bottom panel). The boxes to the right of the graphs show calculated EC50 values. Figures 21A and 21Bshow binding of human monoclonal antibody S2X1and comparator antibody S2X200 to SARS-CoV-1 Spike protein, SARS-CoV-1 Spike protein RED, and SARS-C0V-2 Spike protein RED. Human monoclonal antibodies were expressed recombinantly and binding was tested by ELISA. Figure 21A shows binding of antibodies to SARS-CoV-1 Spike protein RED (top panel) and SARS-C0V- Spike protein (bottom panel). Figure 21B shows binding of antibodies to SARS- C0V-2 Spike protein RBD (top panel) and to an uncoated control plate (bottom panel). The box to the right of the top graph in Figure 21B shows calculated EC50 values for binding SARS-C0V-2 RBD. Figures 22A and 22Bshow binding of human monoclonal antibodies S2H101, S2D65 (22A), S2D97, and S2D106 (22B) to SARS-C0V-2 Spike protein RBD. Antibodies were expressed recombinantly and binding was tested by ELISA. The boxes to the right of the graphs show calculated EC50 values. Figures 23A and 23Bshow antibody inhibition of binding by SARS-C0V-RBD to human ACE2, as measured by ELISA. Figure 23 A shows results for monoclonal antibody S2X149. Figure 24B shows results for monoclonal antibody S2X179 and comparator antibody S2X200. Calculated IC50 values are shown to the right of each graph.

WO 2021/226560 PCT/US2021/031442 Figure 24summarizes results of quantitative epitope-specific serology studies using monoclonal antibody S309 and other anti-Spike antibodies, as determined by binding competition, cryo-EM, and crystallography data. Underlined antibodies are cross-reactive with SARS-CoV-1. Figures 25A and 25Bshow neutralization of SARS-CoV-2 infection by certain monoclonal antibodies. Figure 25A shows results for four antibodies of the present disclosure, along with comparator antibodies S309 N55Q LS and S2X193. S309 N55Q LS comprises the VH amino acid sequence set forth in SEQ ID NO:342 and the VL amino acid sequence set forth in SEQ ID NO: 346, and comprises an MLNS modification in the Fc region. Figure 25B shows results for antibodies S2X129 and S2X132, along with four comparator antibodies. Calculated IC50 values are shown in the boxes below each graph. Calculated EC50 and EC90 values are shown at the bottom of each figure. Figure 26shows neutralization of SARS-CoV-2 infection by certain monoclonal antibodies using a VSV pseudovirus. Data are from one single experiment, triplicate wells VSV-luc(spike D19) pseudovirus. "LS" = Fc mutations M428L + N434S. Figure 27shows neutralization of infection by live SARS-CoV-2 by certain monoclonal antibodies. Data are from triplicate wells SARS-C0V-2-luc, MOI 0.1, 6h infection. Figures 28A and 28Bshow activation of FcyRIIIa (V158 allele) (Figure 28A) and FcyRIIa (H131 allele) (Figure 28B) by certain monoclonal antibodies. Data show experiments using CHO target cells expressing SARS C0V2 S protein. Figures 29A and 29Bshow binding (ELISA) of certain antibodies to SARS- C0V-2 RBD and Spike protein. Figures 30A and 30Bshow binding of certain antibodies against SARS-CoV-RBD, SARS-CoV-2 S protein, and SARS-CoV-1 RBD. Figures 31A and 31Bshow binding of antibody S2D106 to SARS-CoV-2 RBD in the presence of different concentrations of AAPH (2,2'-azobis(2-amidinopropane) dihydrochloride) or after UV irradiation. Both AAPH and UV radiation are used to WO 2021/226560 PCT/US2021/031442 induce oxidation stress in the antibody. Figure 31A shows binding of S2D106 to RBD as measured by indirect ELISA. Figure 3 IB shows binding of S2D106 as measured by sandwich ELISA; x-axis = concentration of RBD. Figures 32A-32Cshow neutralization of infection (pseudovirus particles) by S2E12 and engineered variants thereof (see Table 22 in Example 9 for VH and VL sequences of S2E12 antibodies). Figure 32A and Figure 32B show results from two repetitions of the same experiment. Figure 32C shows results for a third experiment using S2E12 and other variants thereof. Figures 33A and 33Bshow binding of certain antibodies to SARS-C0V-2 RBD as measured by indirect ELISA. Figure 33A shows results for eight antibodies. Figure 33B shows the average of results from duplicate experiments for six antibodies. Figures 34A and 34Bshow binding of certain antibodies to SARS-C0V-2 RBD as measured by sandwich ELISA. Figure 34A shows results for eight antibodies. Figure 34B shows the average of results from duplicate experiments for six antibodies. Figure 35shows neutralization of infection using live SARS-C0V-2 virus by five antibodies. The curve labeled "S2E12-1 1" was generated using antibody present in the supernatant of CHO cells transformed to express S2E12 antibody. The curve labeled "S2E12 wt" was generated using purified antibody produced in transformed HEK cells. Figure 36shows binding of certain antibodies to SARS-C0V-2 Spike protein expressed on the surface of CHO cells, as measured by flow cytometry. The calculated EC50 values for each antibody are shown in the legend to the right of each antibody name. The curve labeled "S2E12-1 1" was generated using antibody present in the supernatant of CHO cells transformed to express S2E12 antibody. The curve labeled "S2E12" was generated using purified antibody produced in transformed HEK cells. Figures 37A and 37Bshow neutralization of infection using pseudovirus particles by certain monoclonal antibodies. Figure 37A and Figure 37B show results from two repetitions of the same experiment. Figures 38A and 38Bshow binding of certain antibodies to SARS-C0V-RBD. Binding of comparator antibody S309-14 (S309 with VH W105F mutation, WO 2021/226560 PCT/US2021/031442 having similar affinity to RBD as S309) is also shown. Figure 38A shows binding as measured by indirect ELISA. Figure 38B shows binding as measured by sandwich ELISA. Each of Figures 38A and 38B show the average of results from duplicate experiments. Figures 39A and 39Bshow characteristics of certain engineered S2Eantibodies as compared to parental monoclonal antibody S2E12 (S2E12 values indicated as "WT" on the y-axis). Comparative data were generated using the assays listed in the legend on the right of each figure. Figure 40shows characteristics of certain engineered S2D106 antibodies as compared to parental monoclonal antibody S2D106 (S2D106 values indicated as "WT" on the y-axis). Comparative data were generated using the assays listed in the legend on the right of each figure. Figure 41 shows expression (immunofluorescence) of DC-SIGN/L-SIGN, DC- SIGN, and ACE2 transgenes in HEK293T cells engineered to overexpress the indicated protein(s). See Example 14. Figure 42 shows VSV pseudovirus infection levels in wild-type HEK293T cells and in HEK293T cells engineered to overexpress DC-SIGN, L-SIGN, or ACE2. The pseudovirus expressed a recombinant SARS-CoV-2 spike protein with luciferase reporter. See Example 14. Figure 43 shows neutralization by monoclonal antibody S309 (VH of SEQ ID NO. :139, VL of SEQ ID NO. :143) of VSV pseudovirus infection in HEK293T cells engineered to overexpress DC-SIGN, L-SIGN, or ACE2. In this example, antibody S309 includes M428L and N434S Fc mutations. See Example 14. Figure 44 shows live SARS-CoV-2 infection levels in wild-type HEK293T cells and in HEK293T cells engineered to overexpress DC-SIGN, L-SIGN, or ACE2. Infection was determined using a recombinant S protein with luciferase reporter. See Example 14. Figure 45 shows neutralization by monoclonal antibody S309 (VH of SEQ ID NO.:139, VL of SEQ IDNO.:143) oflive SARS-CoV-2 infection inHEK293T cells WO 2021/226560 PCT/US2021/031442 engineered to overexpress DC-SIGN, L-SIGN, or ACE2. In this example, antibody S309 includes M428L and N434S Fc mutations. See Example 14. Figure 46shows expression (immunofluorescence) of DC-SIGN/L-SIGN, DC- SIGN, SIGLEC1, and ACE2 transgenes in HEK293T cells engineered to overexpress the indicated protein(s). See Example 14. Figure 47shows live SARS-CoV-2 infection levels in wild-type HEK293T cells and in HEK293T cells engineered to overexpress DC-SIGN, L-SIGN, SIGLEC-1, or ACE2. Infection was determined using a recombinant S protein with luciferase reporter. See Example 14. Figure 48shows neutralization by monoclonal antibody S309 (VH of SEQ ID NO.:139, VL of SEQ IDNO.:143) oflive SARS-CoV-2 infection inHEK293T cells engineered to overexpress DC-SIGN, L-SIGN, SIGLEC-1, or ACE2. In this example, antibody S309 includes M428L and N434S Fc mutations. See Example 14. Figure 49shows neutralization by monoclonal antibody S2E12-LS (VH of SEQ ID NO. :399, VL of SEQ ID NO.:403n M428L/N434S Fc mutations) oflive SARS- C0V-2 infection in HEK293T cells engineered to overexpress DC-SIGN, L-SIGN, SIGLEC-1, or ACE2. In this example, antibody S2E12 includes M428L and N434S Fc mutations. See Example 14. Figures 50A and SOBshows expression analysis of receptor proteins including CD209 (DC-SIGN) and SIGLEC proteins in several cell types. Size of dot correlates with the percentage of cells of the indicated type that express the protein, and intensity of dot shading correlates with the expression level of the protein. See Example 14. Figure 51shows infection by live SARS-CoV-2 expressing N-luciferase in HEK293T cells ("parental") or HEK293T cells stably expressing DC-SIGN, L-SIGN, SIGLEC-1, or ACE2. Data represent experiments testing SARS-CoV-2 at three multiplicities of infection (MOI). See Example 14. Figure 52shows infection by SARS-CoV-2 pseudotyped VSV in HEK293T cells, HeLa cells, and MRC5 cells transiently transduced with lentivirus to express DC- SIGN, L-SIGN, SIGLEC-1, or ACE2. Uninfected cells are shown as negative control. See Example 14.

WO 2021/226560 PCT/US2021/031442 Figure 53shows neutralization of infection by S2E12. A panel of 7 cell lines (HeLa, 293T (wt), Vero E6, Huh7, 293T ACE2, MRC 5-ACE2-TMPRSS2, A549- ACE2-TMPRSS2 clone 5, A549-ACE2-TMPRSS2 clone 10) were infected with SARS- C0V-2-Nluc in the presence of S2E12. Luciferase signal was quantified 24h post infection. Figure 54shows neutralization of infection by S2E12. A panel of 7 cell lines (HeLa, 293T (wt), Vero E6, Huh7, 293T ACE2, MRC 5-ACE2-TMPRSS2, A549- ACE2-TMPRSS2 clone 5, A549-ACE2-TMPRSS2 clone 10) were infected with VSV pseudotyped with the SARS-C0V-2 spike protein in the presence of S2E12-LS. Luciferase signal was quantified 24h post infection. Figure 55shows binding of purified, fluorescently-labeled SARS-C0V-2 spike protein binding to each of 7 cell lines as quantified by flow cytometry. HeLa and 239T WT cells had he lowest MFIs, followed by Huh7 and VeroE6 cells. 293T ACE2 cells (highest), MRC 5-ACE2-TMPRSS2 (third-highest), A549-ACE2-TMPRSS2 clone (fourth-highest), and A549-ACE2-TMPRSS2 clone 10 (second-highest) had higher MFIs. Figures 56A and 56Bshow that both S309 (VH SEQ ID NO.: 139; VL SEQ ID NO.: 143) or the combination of S309 and S2E12-LS provide robust in vivo protection against SARS-C0V-2 challenge. Syrian hamsters were injected with the indicated amount of mAb 48 hours before intra-nasal challenge with SARS-C0V-2. Fig. 56A, top row, shows quantification of viral RNA in the lungs 4 days post-infection. Fig 56A, middle row, shows quantification of replicating virus in lung homogenates harvested days post infection using a TCID50 assay. Fig 56A, bottom row, shows histological score of the lung tissue assessed 4 days post infection. Fig. 56B shows that the concentration of mAbs measured in the serum before infection (day 0) inversely correlates with the viral RNA load in the lung 4 days post infection. See Example 14. Figure 57shows infection of HEK293T cells transfected to over-express ACEor one of a panel of selected lectins and receptor candidates by VSV-SARS-C0V-pseudovirus.

WO 2021/226560 PCT/US2021/031442 Figure 58shows micrographs of stable HEK293T cell lines overexpressing DC- SIGN, L-SIGN, SIGLEC1, or ACE2 infected with authentic SARS-CoV-2 (MOI of 0.1), then fixed and immunostained for 24 hours for SARS-CoV-2 nucleoprotein (red) Figure 59shows quantification of luciferase levels in stable HEK293T cell lines overexpressing DC-SIGN, L-SIGN, SIGLEC1, or ACE2, as measured 24 hours after infection with SARS-CoV-2-Nluc. Figure 60shows quantification of luciferase levels in stable HEK293T cell lines overexpressing DC-SIGN, L-SIGN, SIGLEC1, or ACE2 after incubation with different concentrations of anti-SIGLECl monoclonal antibody (clone 7-239) and infection with SARS-C0V-2-Nluc. Figure 61shows infection of cells transiently transduced to overexpress DC- SIGN, L-SIGN, SIGLEC1, or ACE2 by VSV-SARS-CoV-2 pseudovirus. Results for HEK293Tcells (left panel), HeLa cells (center panel), and MRC5cells (right panel) are shown. Figure 62shows infection of stable HEK293T cell lines overexpressing DC- SIGN, L-SIGN, SIGLEC1, or ACE2 after treatment with ACE2 siRNA followed by infection with VSV-SARS-CoV-2 pseudovirus. Figure 63shows infection of stable HEK293T cell lines overexpressing DC- SIGN, L-SIGN, SIGLEC1, or ACE2 after treatment with different concentrations of anti-ACE2 antibody (polyclonal serum) followed by infection with VSV-SARS-CoV-pseudovirus. Figure 64shows the distribution and expression of ACE2, DC-SIGN (CD209), L-SIGN (CLEC4M), and SIGLEC1 in the human lung cell atlas. Figure 65 shows analysis of major cell types with detectable SARS-CoV-genome in bronchoalveolar lavage fluid or sputum of severe COVID-19 patients. The single cell gene expression profiles are shown as a t-SNE (!-distributed stochastic neighbor embedding) plot, colored by cell type and sized by viral load. Figure 66 shows analysis of major cell types with detectable SARS-CoV-genome in bronchoalveolar lavage fluid or sputum of severe COVID-19 patients. The cumulative fraction of cells (y-axis) with detected viral RNA per cell up to the WO 2021/226560 PCT/US2021/031442 corresponding logCPM (log(counts per million); x-axis) is shown for each of the indicated cell types. Figure 67 shows a heatmap matrix of counts for cells with detected transcripts for the receptor genes shown on the x-axis and SARS-CoV-2+ cell types on the y-axis. Total n=3,085 cells from eight subjects. See Ren, X. et al. COVID-19 immune features revealed by a large-scale single cell transcriptome atlas. Cell, doi:10.1016/j.cell. 2021.01.053 (2021). Figure 68 shows the correlation of receptor transcript counts (y-axis of each plot) with SARS-C0V-2 RNA counts (x-axis of each plot) in macrophages and in secretory cells. Correlation is based on counts before log transformation from Ren et al. Figure 69 shows, at right, results of trans-infection with VSV-SARS-C0V-2. A schematic of the trans-infection process is shown in the left panel. HeLa cells transduced with DC-SIGN, L-SIGN, or SIGLEC1 were incubated with VSV-SARS- C0V-2, extensively washed, and co-cultured with Vero-E6-TMPRSS2 susceptible target cells. Results in the presence or absence of target cells are shown in the right panel. Figure 70 shows the results of trans-infection, where VSV-SARS-C0V-2 viral adsorption was performed in the presence or absence of an anti-SIGLECl blocking antibody. Figure 71 shows neutralization of SARS-C0V-2 infection of Vero-E6 cells by antibodies S309, S2E12-LS, and S2X33. S2E12-LS comprises the VH sequence of SEQ ID NO:399 and the VL sequence of SEQ ID NO:403, and M428L/N434S in Fc. Figure 72 shows neutralization of SARS-C0V-2 infection of Vero-E6- TMPRSS2 cells by antibodies S309, S2E12-LS, and S2X33. Figure 73 shows quantification of binding of purified, fluorescently-labeled SARS-C0V-2 spike protein or RED to the indicated cell lines, as measured by flow cytometry. "A" indicates cell line overexpressing ACE2; "T" indicates cell line overexpressing TMPRSS2.

WO 2021/226560 PCT/US2021/031442 Figure 74 shows quantification of cellular ACE2 and TMPRSS2 transcripts in the indicated cell lines, as measured by RT-qPCR. "A" indicates cell line overexpressing ACE2; "T" indicates cell line overexpressing TMPRSS2. Figure 75 shows neutralization of SARS-C0V-2-Nluc infection by antibodies S309, S2E12-LS, or S2X333. Each of the seven cell lines indicated was tested. Luciferase signal was quantified 24 hours post infection. Figure 76 shows neutralization of VSV-SARS-C0V-2 pseudovirus infection by antibodies S309, S2E12-LS, or S2X333. Each of the seven cell lines indicated was tested. Luciferase signal was quantified 24 hours post infection. Figure77 shows cell-cell fusion of CHO cells expressing SARS-C0V-2 S protein (CHO-S) on the plasma membrane in the absence (top panels) or presence (bottom panels) of 5 ug/ml of antibody S2E12-LS, as measured by immune- fluorescence. Nuclei were stained with Hoechst dye; cytoplasm was stained with CellTracker Green. Figure 78 shows CHO-S cell-cell fusion mediated by different spike-specific antibodies. Fusion was quantified using the Cytation 5 Imager (BioTek) and an object detection protocol that detected nuclei as objects and measured their size. The area of the objects in fused cells divided by the total area of all the objects multiplied by 1provides the percentage of fused cells. Figure 79shows inhibition of S2E12-LS -induced cell-cell fusion of CHO-S cells by 15 ug/ml of the indicated antibodies. Figure 80shows S2E12-LS -induced uni-directional fusion (also referred to as trans-fusion) of S-positive CHO-S cells with fluorescently-labelled S-negative CHO cells in the absence of ACE2. Nuclei were stained with Hoechst dye; cytoplasm was stained with CellTracker Green. Figure 81shows neutralization of infection of a stable HEK293T cell line overexpressing ACE2 by authentic SARS-C0V-2 pre-incubated with the indicated antibodies. Infection was measured by immunostaining at 24 hours for the SARS-C0V- nucleoprotein.

WO 2021/226560 PCT/US2021/031442 Figure 82shows neutralization of infection of a stable HEK293T cell line overexpressing SIGLEC1 by authentic SARS-CoV-2 pre-incubated with the indicated antibodies. Infection was measured by immunostaining at 24 hours for the SARS-CoV- nucleoprotein. Figure 83shows neutralization of infection of a stable HEK293T cell line overexpressing DC-SIGN by authentic SARS-CoV-2 pre-incubated with the indicated monoclonal antibodies. Infection was measured by immunostaining at 24 hours for the SARS-CoV-2 nucleoprotein. Figure 84shows neutralization of infection of a stable HEK293T cell line overexpressing L-SIGN by authentic SARS-CoV-2 pre-incubated with the indicated monoclonal antibodies. Infection was measured by immunostaining at 24 hours for the SARS-CoV-2 nucleoprotein. Figure 85shows a summary of the mechanisms of action of different classes of spike-specific antibodies. "Fusion inhibition" refers to antibody-mediated inhibition of fusion between CHO-S cells and ACE2+ Vero-E6 cells. Assessment of effector functions is based on antibody-dependent activation of human FcyRs, as measured using a bioluminescent reporter assay. RBM la-IIa antibodies include S2E12, S2X259, S2X58, S2D106, Ly-CoV016, CT-P59, and REGN10933. RBM lb antibodies include Ly-C0V555, REGN10987, and S2M11. NTD antibodies include S2X333. Stem helix antibodies include S2P6. Figure 86 shows analysis of binding of antibodies targeting DC/L-SIGN, DC- SIGN, SIGLEC1, or ACE2 on HEK293T cells stably over-expressing the respective attachment receptor, as measured by flow cytometry. Figure 87 shows analysis of binding of antibodies targeting DC/L-SIGN, DC- SIGN, SIGLEC1, or ACE2 on HEK293T cells stably over-expressing the respective attachment receptor, as measured by immunofluorescence. Figure 88 shows infection of HEK293T cells stably over-expressing the indicated attachment receptor by VSV-SARS-C0V-2 pseudotyped with wild type spike protein (grey bars), or VSV-SARS-C0V-2 pseudotyped with spike protein bearing the WO 2021/226560 PCT/US2021/031442 mutations of the B1.1.7 lineage (red bars). Luminescence was analyzed one day post infection. Figure 89 shows neutralization of SARS-CoV-2 infection of Vero-E6 or Vero- E6-TMPRSS2 cells by 10 pg/ml of S309, S2E12-v2, and S2X333. Cells were infected with SARS-CoV-2 (isolate USA-WA1/2020) at MOI 0.01 in the presence of the indicated antibodies. Cells were fixed 24h post infection and viral nucleocapsid protein was immunostained. Figure 90 shows quantification of binding of purified, fluorescently-labelled SARS-CoV-2 spike protein (left panels) or RBD (right panels) to the indicated cell lines, as measured by flow cytometry. Figure 91shows quantification of binding of purified, fluorescently-labelled SARS-CoV-2 spike protein (left panels) or RBD (right panels) to the indicated cell lines, as measured by flow cytometry. Figure 92shows analysis of the protective effect of antibody S309 (left panels) or a combination of antibodies S309 and S2E12-v2 (right panels) against SARS-CoV-challenge in Syrian hamsters. Top panels show quantification of viral RNA in the lungs four days post infection. Bottom panels show quantification of replicating virus in lung homogenates harvested four days post infection using a TCID50 assay. Figure 93shows analysis of the protective effect of antibody S309 (left panels) or a combination of antibodies S309 and S2E12-LS (right panels) against SARS-CoV-challenge in Syrian hamsters. Top panels show histopathological score of lung tissue assessed four days post infection. Bottom panels show efficacy plots based on the correlation between the level of serum antibody measured at the time of infection (x- axis) and the level of SARS-CoV-2 viral RNA measured in lungs (y-axis) on day four after infection. The dotted lines represent the EC50 and EC90 for viral reduction. EC90 of S309 alone is 9 pg/ml; EC90 of S309+S2E12-v2 is 11 pg/ml. Figure 94shows binding of immunocomplexes to hamster splenocytes. Alexa- 488 fluorescent immunocomplexes (IC) were titrated (0-200 nM range) and incubated with total naive hamster splenocytes. Binding was revealed with a cytometer upon exclusion of dead/apoptotic cells and physical gating on bona fide monocyte WO 2021/226560 PCT/US2021/031442 population. Left panel shows the fluorescent intensity associated to hamster cells of IC made with either hamster or human Fc antibodies (Human S309 shown in green; GH- S3 09 shown in dark grey; GH-S309-N297A shown in blue). A single replicate of two is shown. Right panel shows the relative Alexa-488 mean fluorescent intensity of the replicates measured on the entire monocyte population. Figure 95shows analysis of the role of host effector function in SARS-C0V-challenge. Syrian hamsters were injected with the indicated amount (mg/kg) of hamster IgG2a S309, either wt or Fc silenced (S309-N297A). Top panel shows quantification of viral RNA in the lung 4 days post infection. Center panel shows quantification of replicating virus in the lung 4 days post infection. Bottom panel shows histopathological score in the lung 4 days post infection. Control animals (white symbols) were injected with 4 mg/kg unrelated control isotype antibody. * p< 0.05, ** p< 0.01, *** p< 0.001, **** p< 0.0001 vs control animals, using Mann-Whitney test. Figure 96shows inhibition of infection by SARS-C0V-2-Nluc in HeLa cells stably expressing DC-SIGN in the presence of the indicated antibodies. Cells were infected at MOI of 0.04. Infection was analyzed by quantification of luminescent signal at 24 hours post infection. Figure 97shows neutralization of SARS-C0V-2 infection of HEK293T cells stably expressing ACE2 (top panel) or DC-SIGN (bottom panel) in the presence of the indicated antibodies. Cells were infected at MOI of 0.02. Cells were fixed 24h post infection, viral nucleocapsid protein was immunostained and positive cells were quantified. Figure 98shows neutralization of SARS-C0V-2 infection of HEK293T cells stably expressing SIGLEC1 (top panel) or L-SIGN (bottom panel) in the presence of the indicated antibodies. Cells were infected at MOI of 0.02. Cells were fixed 24h post infection, viral nucleocapsid protein was immunostained and positive cells were quantified. Figure 99shows a concentration curve for S2E12 engineered variant 409 1 l_4_v2, comprising LS mutations in Fc, in female non-human primates over days.

WO 2021/226560 PCT/US2021/031442 Figure 100shows 409 1 l_4_v2-LS pharmacokinetics values from the non- human primate study.

DETAILED DESCRIPTION Provided herein are antibodies and antigen-binding fragments that bind to SARS-CoV-2 coronavirus (e.g., a SARS-CoV-2 surface glycoprotein and/or RED, as described herein, in a SARS-CoV-2 virion and/or expressed on the surface of a cell infected by the SARS-CoV-2 coronavirus). In certain embodiments, presently disclosed antibodies and antigen-binding fragments can neutralize a SARS-CoV-infection in an in vitro model of infection and/or in an animal model of infection and/or in a human subject. Also provided are polynucleotides that encode the antibodies and antigen-binding fragments, vectors, host cells, and related compositions, as well as methods of using the antibodies, nucleic acids, vectors, host cells, and related compositions to treat (e.g, reduce, delay, eliminate, or prevent) a SARS-CoV-infection in a subject and/or in the manufacture of a medicament for treating a SARS- C0V-2 infection in a subject.Prior to setting forth this disclosure in more detail, it may be helpful to an understanding thereof to provide definitions of certain terms to be used herein. Additional definitions are set forth throughout this disclosure.As used herein, "SARS-CoV-2", also referred to herein as "Wuhan seafood market phenomia virus", or "Wuhan coronavirus" or "Wuhan C0V", or "novel C0V", or "nCoV", or "2019 nCoV", or "Wuhan nCoV" is a betacoronavirus believed to be of lineage B (sarbecovirus). SARS-CoV-2 was first identified in Wuhan, Hubei province, China, in late 2019 and spread within China and to other parts of the world by early 2020. Symptoms of SARS-CoV-2 infection include fever, dry cough, and dyspnea.The genomic sequence of SARS-CoV-2 isolate Wuhan-Hu- 1 is provided in SEQ ID NO.:1 (see also GenBank MN908947.3, January 23, 2020), and the amino acid translation of the genome is provided in SEQ ID NO. :2 (see also GenBank QHD43416.1, January 23, 2020). Like other coronaviruses (e.g, SARS- C0V-1), SARS-CoV-2 comprises a "spike" or surface ("S") type I transmembrane glycoprotein WO 2021/226560 PCT/US2021/031442 containing a receptor binding domain (RBD). RBD is believed to mediate entry of the lineage B SARS coronavirus to respiratory epithelial cells by binding to the cell surface receptor angiotensin-converting enzyme 2 (ACE2). In particular, a receptor binding motif (RBM) in the virus RBD is believed to interact with ACE2.The amino acid sequence of the Wuhan-Hu- 1 surface glycoprotein is provided in SEQ ID NO.:3. The amino acid sequence of Wuhan-Hu- 1 RBD is provided in SEQ ID NO. :4. SARS-CoV-2 S protein has approximately 73% amino acid sequence identity with SARS-CoV-1. The amino acid sequence of Wuhan-Hu- 1 RBM is provided in SEQ ID NO.:5. Wuhan-Hu- 1 RBD has approximately 75% to 77% amino acid sequence similarity to SARS-CoV-1 RBD, and Wuhan-Hu- 1 RBM has approximately 50% amino acid sequence similarity to SARS-CoV-1 RBM.Unless otherwise indicated herein, SARS-CoV-2 Wuhan Hu-1 refers to a virus comprising the amino acid sequence set forth in any one or more of SEQ ID NOs.:2, 3, and 4, optionally with the genomic sequence set forth in SEQ ID NO.:1.There have been a number of emerging SARS-CoV-2 variants. Some SARS- C0V-2 variants contain an N439K mutation, which has enhanced binding affinity to the human ACE2 receptor (Thomson, E.C., et al., The circulating SARS-CoV-2 spike variant N439K maintains fitness while evading antibody-mediated immunity. bioRxiv, 2020). Some SARS-CoV-2 variants contain an N501 ¥ mutation, which is associated with increased transmissibility, including the lineages B.l.1.7 (also known as 20I/501Y.V1 and VOC 202012/01; (del69-70, dell44, N501Y, A570D, D614G, P681H, T716I, S982A, and DI 118H mutations)) andB. 1.351 (also known as 20H/501Y.V2; L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, and A701V mutations), which were discovered in the United Kingdom and South Africa, respectively (Tegally, H., et al., Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple spike mutations in South Africa. medRxiv, 2020: p. 2020.12.21.20248640; Leung, K., et al., Early empirical assessment of the N501Y mutant strains of SARS-CoV-2 in the United Kingdom, October to November 2020. medRxiv, 2020: p. 2020.12.20.20248581). B. 1.351 also include two other mutations in the RBD domain of SARS-C0V2 spike WO 2021/226560 PCT/US2021/031442 protein, K417N and E484K (Tegally, H., et al., Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple spike mutations in South Africa. medRxiv, 2020: p. 2020.12.21.20248640). Other SARS-CoV-2 variants include the Lineage B. 1.1.28, which was first reported in Brazil; the Variant P.l, lineage B. 1.1.28 (also known as 20J/501Y.V3), which was first reported in Japan; Variant L452R, which was first reported in California in the United States (Pan American Health Organization, Epidemiological update: Occurrence of variants of SARS-CoV-2 in the Americas, January 20, 2021, available at reliefweb.int/sites/reliefweb.int/f11es/resources/2021-jan-20-phe-epi-update-SARS- C0V-2.pdf). Other SARS-CoV-2 variants include a SARS C0V-2 of clade 19A; SARS C0V-2 of clade 19B; a SARS C0V-2 of clade 20A; a SARS C0V-2 of clade 20B; a SARS C0V-2 of clade 20C; a SARS C0V-2 of clade 20D; a SARS C0V-2 of clade 20E (EUI); a SARS C0V-2 of clade 20F; a SARS C0V-2 of clade 20G; and SARS C0V-Bl. 1.207; and other SARS C0V-2 lineages described in Rambaut, A., etal., A dynamic nomenclature proposal for SARS-CoV-2 lineages to assist genomic epidemiology. Nat Microbiol 5, 1403-1407 (2020). The foregoing SARS-CoV-2 variants, and the amino acid and nucleotide sequences thereof, are incorporated herein by reference.Accordingly, it will be understood that SARS-CoV-2 includes Wuhan Hu-1 and variants thereof, including presently disclosed variants.In the present description, any concentration range, percentage range, ratio range, or integer range is to be understood to include the value of any integer within the recited range and, when appropriate, fractions thereof (such as one tenth and one hundredth of an integer), unless otherwise indicated. Also, any number range recited herein relating to any physical feature, such as polymer subunits, size or thickness, are to be understood to include any integer within the recited range, unless otherwise indicated. As used herein, the term "about" means ± 20% of the indicated range, value, or structure, unless otherwise indicated. It should be understood that the terms "a" and "an" as used herein refer to "one or more" of the enumerated components. The use of the alternative (e.g., "or") should be understood to mean either one, both, or any combination thereof of the alternatives. As used herein, the terms "include," "have," WO 2021/226560 PCT/US2021/031442 and "comprse" are used synonymously, which terms and variants thereof are intended to be construed as non-limiting."Optional" or "optionally" means that the subsequently described element, component, event, or circumstance may or may not occur, and that the description includes instances in which the element, component, event, or circumstance occurs and instances in which they do not.In addition, it should be understood that the individual constructs, or groups of constructs, derived from the various combinations of the structures and subunits described herein, are disclosed by the present application to the same extent as if each construct or group of constructs was set forth individually. Thus, selection of particular structures or particular subunits is within the scope of the present disclosure.The term "consisting essentially of is not equivalent to "comprising" and refers to the specified materials or steps of a claim, or to those that do not materially affect the basic characteristics of a claimed subject matter. For example, a protein domain, region, or module (e.g., a binding domain) or a protein "consists essentially of a particular amino acid sequence when the amino acid sequence of a domain, region, module, or protein includes extensions, deletions, mutations, or a combination thereof (e.g., amino acids at the amino- or carboxy-terminus or between domains) that, in combination, contribute to at most 20% (e.g., at most 15%, 10%, 8%, 6%, 5%, 4%, 3%, 2% or 1%) of the length of a domain, region, module, or protein and do not substantially affect (i.e., do not reduce the activity by more than 50%, such as no more than 40%, 30%, 25%, 20%, 15%, 10%, 5%, or 1%) the activity of the domain(s), region(s), module(s), or protein (e.g., the target binding affinity of a binding protein).As used herein, "amino acid" refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids. Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, y-carboxyglutamate, and O-phosphoserine. Amino acid analogs refer to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an a-carbon that is bound to a hydrogen, a carboxyl group, an amino WO 2021/226560 PCT/US2021/031442 group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid. Amino acid mimetics refer to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally occurring amino acid.As used herein, "mutation" refers to a change in the sequence of a nucleic acid molecule or polypeptide molecule as compared to a reference or wild-type nucleic acid molecule or polypeptide molecule, respectively. A mutation can result in several different types of change in sequence, including substitution, insertion or deletion of nucleotide(s) or amino acid(s).A "conservative substitution" refers to amino acid substitutions that do not significantly affect or alter binding characteristics of a particular protein. Generally, conservative substitutions are ones in which a substituted amino acid residue is replaced with an amino acid residue having a similar side chain. Conservative substitutions include a substitution found in one of the following groups: Group 1: Alanine (Ala or A), Glycine (Gly or G), Serine (Ser or S), Threonine (Thr or T); Group 2: Aspartic acid (Asp or D), Glutamic acid (Glu or Z); Group 3: Asparagine (Asn or N), Glutamine (Gin or Q); Group 4: Arginine (Arg or R), Lysine (Lys or K), Histidine (His or H); Group 5: Isoleucine (He or I), Leucine (Leu or L), Methionine (Met or M), Valine (Vai or V); and Group 6: Phenylalanine (Phe or F), Tyrosine (Tyr or Y), Tryptophan (Trp or W). Additionally or alternatively, amino acids can be grouped into conservative substitution groups by similar function, chemical structure, or composition (e.g., acidic, basic, aliphatic, aromatic, or sulfur-containing). For example, an aliphatic grouping may include, for purposes of substitution, Gly, Ala, Vai, Leu, and He. Other conservative substitutions groups include: sulfur-containing: Met and Cysteine (Cys or C); acidic: Asp, Glu, Asn, and Gin; small aliphatic, nonpolar or slightly polar residues: Ala, Ser, Thr, Pro, and Gly; polar, negatively charged residues and their amides: Asp, Asn, Glu, and Gin; polar, positively charged residues: His, Arg, and Lys; large aliphatic, nonpolar residues: Met, Leu, He, Vai, and Cys; and large aromatic residues: Phe, Tyr, and Trp.

WO 2021/226560 PCT/US2021/031442 Additional information can be found in Creighton (1984) Proteins, W.H. Freeman and Company.As used herein, "protein" or "polypeptide" refers to a polymer of amino acid residues. Proteins apply to naturally occurring amino acid polymers, as well as to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, and non-naturally occurring amino acid polymers. Variants of proteins, peptides, and polypeptides of this disclosure are also contemplated. In certain embodiments, variant proteins, peptides, and polypeptides comprise or consist of an amino acid sequence that is at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 99.9% identical to an amino acid sequence of a defined or reference amino acid sequence as described herein."Nucleic acid molecule" or "polynucleotide" or "polynucleic acid" refers to a polymeric compound including covalently linked nucleotides, which can be made up of natural subunits (e.g., purine or pyrimidine bases) or non-natural subunits (e.g., morpholine ring). Purine bases include adenine, guanine, hypoxanthine, and xanthine, and pyrimidine bases include uracil, thymine, and cytosine. Nucleic acid molecules include polyribonucleic acid (RNA), which includes mRNA, microRNA, siRNA, viral genomic RNA, and synthetic RNA, and polydeoxyribonucleic acid (DNA), which includes cDNA, genomic DNA, and synthetic DNA, either of which may be single or double stranded. If single-stranded, the nucleic acid molecule may be the coding strand or non-coding (anti-sense) strand. A nucleic acid molecule encoding an amino acid sequence includes all nucleotide sequences that encode the same amino acid sequence. Some versions of the nucleotide sequences may also include intron(s) to the extent that the intron(s) would be removed through co- or post-transcriptional mechanisms. In other words, different nucleotide sequences may encode the same amino acid sequence as the result of the redundancy or degeneracy of the genetic code, or by splicing.Variants of nucleic acid molecules of this disclosure are also contemplated. Variant nucleic acid molecules are at least 70%, 75%, 80%, 85%, 90%, and are preferably 95%, 96%, 97%, 98%, 99%, or 99.9% identical a nucleic acid molecule of a WO 2021/226560 PCT/US2021/031442 defined or reference polynucleotide as described herein, or that hybridize to a polynucleotide under stringent hybridization conditions of 0.015M sodium chloride, 0.0015M sodium citrate at about 65-68°C or 0.015M sodium chloride, 0.0015M sodium citrate, and 50% formamide at about 42°C. Nucleic acid molecule variants retain the capacity to encode a binding domain thereof having a functionality described herein, such as binding a target molecule."Percent sequence identity" refers to a relationship between two or more sequences, as determined by comparing the sequences. Preferred methods to determine sequence identity are designed to give the best match between the sequences being compared. For example, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment). Further, non-homologous sequences may be disregarded for comparison purposes. The percent sequence identity referenced herein is calculated over the length of the reference sequence, unless indicated otherwise. Methods to determine sequence identity and similarity can be found in publicly available computer programs. Sequence alignments and percent identity calculations may be performed using a BLAST program (e.g., BLAST 2.0, BLASTP, BLASTN, or BLASTX). The mathematical algorithm used in the BLAST programs can be found in Altschul et al., Nucleic Acids Res. 25:3389-3402, 1997. Within the context of this disclosure, it will be understood that where sequence analysis software is used for analysis, the results of the analysis are based on the "default values" of the program referenced. "Default values" mean any set of values or parameters which originally load with the software when first initialized.The term "isolated" means that the material is removed from its original environment (e.g, the natural environment if it is naturally occurring). For example, a naturally occurring nucleic acid or polypeptide present in a living animal is not isolated, but the same nucleic acid or polypeptide, separated from some or all of the co-existing materials in the natural system, is isolated. Such nucleic acid could be part of a vector and/or such nucleic acid or polypeptide could be part of a composition (e.g, a cell WO 2021/226560 PCT/US2021/031442 lysate), and still be isolated in that such vector or composition is not part of the natural environment for the nucleic acid or polypeptide.The term "gene" means the segment of DNA or RNA involved in producing a polypeptide chain; in certain contexts, it includes regions preceding and following the coding region (e.g., 5’ untranslated region (UTR) and 3’ UTR) as well as intervening sequences (introns) between individual coding segments (exons).A "functional variant" refers to a polypeptide or polynucleotide that is structurally similar or substantially structurally similar to a parent or reference compound of this disclosure, but differs slightly in composition (e.g., one base, atom or functional group is different, added, or removed), such that the polypeptide or encoded polypeptide is capable of performing at least one function of the parent polypeptide with at least 50% efficiency, preferably at least 55%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.9%, or 100% level of activity of the parent polypeptide. In other words, a functional variant of a polypeptide or encoded polypeptide of this disclosure has "similar binding," "similar affinity" or "similar activity" when the functional variant displays no more than a 50% reduction in performance in a selected assay as compared to the parent or reference polypeptide, such as an assay for measuring binding affinity (e.g., Biacore® or tetramer staining measuring an association (Ka) or a dissociation (Kd) constant).As used herein, a "functional portion" or "functional fragment" refers to a polypeptide or polynucleotide that comprises only a domain, portion or fragment of a parent or reference compound, and the polypeptide or encoded polypeptide retains at least 50% activity associated with the domain, portion or fragment of the parent or reference compound, preferably at least 55%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.9%, or 100% level of activity of the parent polypeptide, or provides a biological benefit (e.g., effector function). A "functional portion" or "functional fragment" of a polypeptide or encoded polypeptide of this disclosure has "similar binding" or "similar activity" when the functional portion or fragment displays no more than a 50% reduction in performance in a selected assay as compared to the WO 2021/226560 PCT/US2021/031442 parent or reference polypeptide (preferably no more than 20% or 10%, or no more than a log difference as compared to the parent or reference with regard to affinity).As used herein, the term "engineered," "recombinant," or "non-natural" refers to an organism, microorganism, cell, nucleic acid molecule, or vector that includes at least one genetic alteration or has been modified by introduction of an exogenous or heterologous nucleic acid molecule, wherein such alterations or modifications are introduced by genetic engineering (i.e., human intervention). Genetic alterations include, for example, modifications introducing expressible nucleic acid molecules encoding functional RNA, proteins, fusion proteins or enzymes, or other nucleic acid molecule additions, deletions, substitutions, or other functional disruption of a cell ’s genetic material. Additional modifications include, for example, non-coding regulatory regions in which the modifications alter expression of a polynucleotide, gene, or operon.As used herein, "heterologous" or "non-endogenous" or "exogenous" refers to any gene, protein, compound, nucleic acid molecule, or activity that is not native to a host cell or a subject, or any gene, protein, compound, nucleic acid molecule, or activity native to a host cell or a subject that has been altered. Heterologous, non-endogenous, or exogenous includes genes, proteins, compounds, or nucleic acid molecules that have been mutated or otherwise altered such that the structure, activity, or both is different as between the native and altered genes, proteins, compounds, or nucleic acid molecules. In certain embodiments, heterologous, non-endogenous, or exogenous genes, proteins, or nucleic acid molecules (e.g., receptors, ligands, etc.) may not be endogenous to a host cell or a subject, but instead nucleic acids encoding such genes, proteins, or nucleic acid molecules may have been added to a host cell by conjugation, transformation, transfection, electroporation, or the like, wherein the added nucleic acid molecule may integrate into a host cell genome or can exist as extra-chromosomal genetic material (e.g., as a plasmid or other self-replicating vector). The term "homologous" or "homolog" refers to a gene, protein, compound, nucleic acid molecule, or activity found in or derived from a host cell, species, or strain. For example, a heterologous or exogenous polynucleotide or gene encoding a polypeptide may be homologous to a WO 2021/226560 PCT/US2021/031442 native polynucleotide or gene and encode a homologous polypeptide or activity, but the polynucleotide or polypeptide may have an altered structure, sequence, expression level, or any combination thereof. A non-endogenous polynucleotide or gene, as well as the encoded polypeptide or activity, may be from the same species, a different species, or a combination thereof.In certain embodiments, a nucleic acid molecule or portion thereof native to a host cell will be considered heterologous to the host cell if it has been altered or mutated, or a nucleic acid molecule native to a host cell may be considered heterologous if it has been altered with a heterologous expression control sequence or has been altered with an endogenous expression control sequence not normally associated with the nucleic acid molecule native to a host cell. In addition, the term "heterologous" can refer to a biological activity that is different, altered, or not endogenous to a host cell. As described herein, more than one heterologous nucleic acid molecule can be introduced into a host cell as separate nucleic acid molecules, as a plurality of individually controlled genes, as a polycistronic nucleic acid molecule, as a single nucleic acid molecule encoding a fusion protein, or any combination thereof.As used herein, the term "endogenous" or "native" refers to a polynucleotide, gene, protein, compound, molecule, or activity that is normally present in a host cell or a subject.The term "expression", as used herein, refers to the process by which a polypeptide is produced based on the encoding sequence of a nucleic acid molecule, such as a gene. The process may include transcription, post-transcriptional control, post-transcriptional modification, translation, post-translational control, post- translational modification, or any combination thereof. An expressed nucleic acid molecule is typically operably linked to an expression control sequence (e.g., a promoter).The term "operably linked" refers to the association of two or more nucleic acid molecules on a single nucleic acid fragment so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of affecting the expression of that coding sequence (i.e., the coding sequence is WO 2021/226560 PCT/US2021/031442 under the transcriptional control of the promoter). "Unlinked" means that the associated genetic elements are not closely associated with one another and the function of one does not affect the other.As described herein, more than one heterologous nucleic acid molecule can be introduced into a host cell as separate nucleic acid molecules, as a plurality of individually controlled genes, as a polycistronic nucleic acid molecule, as a single nucleic acid molecule encoding a protein (e.g., a heavy chain of an antibody), or any combination thereof. When two or more heterologous nucleic acid molecules are introduced into a host cell, it is understood that the two or more heterologous nucleic acid molecules can be introduced as a single nucleic acid molecule (e.g., on a single vector), on separate vectors, integrated into the host chromosome at a single site or multiple sites, or any combination thereof. The number of referenced heterologous nucleic acid molecules or protein activities refers to the number of encoding nucleic acid molecules or the number of protein activities, not the number of separate nucleic acid molecules introduced into a host cell.The term "construct" refers to any polynucleotide that contains a recombinant nucleic acid molecule (or, when the context clearly indicates, a fusion protein of the present disclosure). A (polynucleotide) construct may be present in a vector (e.g., a bacterial vector, a viral vector) or may be integrated into a genome. A "vector" is a nucleic acid molecule that is capable of transporting another nucleic acid molecule. Vectors may be, for example, plasmids, cosmids, viruses, a RNA vector or a linear or circular DNA or RNA molecule that may include chromosomal, non-chromosomal, semi-synthetic or synthetic nucleic acid molecules. Vectors of the present disclosure also include transposon systems (e.g., Sleeping Beauty, see, e.g., Geurts etaL, Mol. Ther. 8:108, 2003: Mates et al., Nat. Genet. 412009 ,753.׳ ). Exemplary vectors are those capable of autonomous replication (episomal vector), capable of delivering a polynucleotide to a cell genome (e.g., viral vector), or capable of expressing nucleic acid molecules to which they are linked (expression vectors).As used herein, "expression vector" or "vector" refers to a DNA construct containing a nucleic acid molecule that is operably linked to a suitable control sequence WO 2021/226560 PCT/US2021/031442 capable of effecting the expression of the nucleic acid molecule in a suitable host. Such control sequences include a promoter to effect transcription, an optional operator sequence to control such transcription, a sequence encoding suitable mRNA ribosome binding sites, and sequences which control termination of transcription and translation. The vector may be a plasmid, a phage particle, a virus, or simply a potential genomic insert. Once transformed into a suitable host, the vector may replicate and function independently of the host genome, or may, in some instances, integrate into the genome itself or deliver the polynucleotide contained in the vector into the genome without the vector sequence. In the present specification, "plasmid," "expression plasmid," "virus," and "vector" are often used interchangeably.The term "introduced" in the context of inserting a nucleic acid molecule into a cell, means "transfection", "transformation," or "transduction" and includes reference to the incorporation of a nucleic acid molecule into a eukaryotic or prokaryotic cell wherein the nucleic acid molecule may be incorporated into the genome of a cell (e.g., chromosome, plasmid, plastid, or mitochondrial DNA), converted into an autonomous replicon, or transiently expressed (e.g., transfected mRNA).In certain embodiments, polynucleotides of the present disclosure may be operatively linked to certain elements of a vector. For example, polynucleotide sequences that are needed to effect the expression and processing of coding sequences to which they are ligated may be operatively linked. Expression control sequences may include appropriate transcription initiation, termination, promoter, and enhancer sequences; efficient RNA processing signals such as splicing and polyadenylation signals; sequences that stabilize cytoplasmic mRNA; sequences that enhance translation efficiency (i.e., Kozak consensus sequences); sequences that enhance protein stability; and possibly sequences that enhance protein secretion. Expression control sequences may be operatively linked if they are contiguous with the gene of interest and expression control sequences that act in trans or at a distance to control the gene of interest.In certain embodiments, the vector comprises a plasmid vector or a viral vector (e.g., a lentiviral vector or a y-retroviral vector). Viral vectors include retrovirus, WO 2021/226560 PCT/US2021/031442 adenovirus, parvovirus (e.g., adeno-associated viruses), coronavirus, negative strand RNA viruses such as ortho-myxovirus (e.g., influenza virus), rhabdovirus (e.g., rabies and vesicular stomatitis virus), paramyxovirus (e.g., measles and Sendai), positive strand RNA viruses such as picornavirus and alphavirus, and double-stranded DNA viruses including adenovirus, herpesvirus (e.g., Herpes Simplex virus types 1 and 2, Epstein-Barr virus, cytomegalovirus), and poxvirus (e.g., vaccinia, fowlpox, and canarypox). Other viruses include, for example, Norwalk virus, togavirus, flavivirus, reoviruses, papovavirus, hepadnavirus, and hepatitis virus. Examples of retroviruses include avian leukosis-sarcoma, mammalian C-type, B-type viruses, D type viruses, HTLV-BLV group, lentivirus, spumavirus (Coffin, J. M., Retroviridae: The viruses and their replication, In Fundamental Virology, Third Edition, B. N. Fields et al., Eds., Lippincott-Raven Publishers, Philadelphia, 1996)."Retroviruses" are viruses having an RNA genome, which is reverse-transcribed into DNA using a reverse transcriptase enzyme, the reverse-transcribed DNA is then incorporated into the host cell genome. "Gammaretrovirus" refers to a genus of the retroviridae family. Examples of gammaretroviruses include mouse stem cell virus, murine leukemia virus, feline leukemia virus, feline sarcoma virus, and avian reticuloendotheliosis viruses."Lentiviral vectors" include HIV-based lentiviral vectors for gene delivery, which can be integrative or non-integrative, have relatively large packaging capacity, and can transduce a range of different cell types. Lentiviral vectors are usually generated following transient transfection of three (packaging, envelope, and transfer) or more plasmids into producer cells. Like HIV, lentiviral vectors enter the target cell through the interaction of viral surface glycoproteins with receptors on the cell surface. On entry, the viral RNA undergoes reverse transcription, which is mediated by the viral reverse transcriptase complex. The product of reverse transcription is a double-stranded linear viral DNA, which is the substrate for viral integration into the DNA of infected cells.In certain embodiments, the viral vector can be a gammaretrovirus, e.g., Moloney murine leukemia virus (MLV)-derived vectors. In other embodiments, the WO 2021/226560 PCT/US2021/031442 viral vector can be a more complex retrovirus-derived vector, e.g., a lentvirus-derved vector. HIV-l-derived vectors belong to this category. Other examples include lentivirus vectors derived from HIV-2, FIV, equine infectious anemia virus, SIV, and Maedi-Visna virus (ovine lentivirus). Methods of using retroviral and lentiviral viral vectors and packaging cells for transducing mammalian host cells with viral particles containing transgenes are known in the art and have been previous described, for example, in: U.S. Patent 8,119,772; Walchli et al., PLoS One 6:327930, 2011; Zhao et al., J. Immunol. 774:4415, 2005; Engels etal., Hum. Gene Ther. 74:1155, 2003; Frecha etal.,Mol. Ther. 18׳ A7A%, 2010; and Verhoeyen et al., Methods Mol. Biol. 506:97, 2009. Retroviral and lentiviral vector constructs and expression systems are also commercially available. Other viral vectors also can be used for polynucleotide delivery including DNA viral vectors, including, for example adenovirus-based vectors and adeno-associated virus (AAV)-based vectors; vectors derived from herpes simplex viruses (HSVs), including amplicon vectors, replication-defective HSV and attenuated HSV (Krisky et al., Gene Ther. 5:1517, 1998).Other vectors that can be used with the compositions and methods of this disclosure include those derived from baculoviruses and a-viruses. (Jolly, D J. 1999. Emerging Viral Vectors, pp 209-40 in Friedmann T. ed. The Development of Human Gene Therapy. New York: Cold Spring Harbor Lab), or plasmid vectors (such as sleeping beauty or other transposon vectors).When a viral vector genome comprises a plurality of polynucleotides to be expressed in a host cell as separate transcripts, the viral vector may also comprise additional sequences between the two (or more) transcripts allowing for bicistronic or multi ci str onic expression. Examples of such sequences used in viral vectors include internal ribosome entry sites (IRES), furin cleavage sites, viral 2A peptide, or any combination thereof.Plasmid vectors, including DNA-based antibody or antigen-binding fragment- encoding plasmid vectors for direct administration to a subject, are described further herein.

WO 2021/226560 PCT/US2021/031442 As used herein, the term "host" refers to a cell or microorganism targeted for genetic modification with a heterologous nucleic acid molecule to produce a polypeptide of interest (e.g., an antibody of the present disclosure).A host cell may include any individual cell or cell culture which may receive a vector or the incorporation of nucleic acids or express proteins. The term also encompasses progeny of the host cell, whether genetically or phenotypically the same or different. Suitable host cells may depend on the vector and may include mammalian cells, animal cells, human cells, simian cells, insect cells, yeast cells, and bacterial cells. These cells may be induced to incorporate the vector or other material by use of a viral vector, transformation via calcium phosphate precipitation, DEAE-dextran, electroporation, microinjection, or other methods. See, for example, Sambrook etal., Molecular Cloning: A Laboratory Manual 2d ed. (Cold Spring Harbor Laboratory, 1989).In the context of a SARS-CoV-2 infection, a "host" refers to a cell or a subject infected with SARS-CoV-2."Antigen" or "Ag", as used herein, refers to an immunogenic molecule that provokes an immune response. This immune response may involve antibody production, activation of specific immunologically-competent cells, activation of complement, antibody dependent cytotoxicicity, or any combination thereof. An antigen (immunogenic molecule) may be, for example, a peptide, glycopeptide, polypeptide, glycopolypeptide, polynucleotide, polysaccharide, lipid, or the like. It is readily apparent that an antigen can be synthesized, produced recombinantly, or derived from a biological sample. Exemplary biological samples that can contain one or more antigens include tissue samples, stool samples, cells, biological fluids, or combinations thereof. Antigens can be produced by cells that have been modified or genetically engineered to express an antigen. Antigens can also be present in a SARS-CoV-2 (e •^•, a surface glycoprotein or portion thereof), such as present in a virion, or expressed or presented on the surface of a cell infected by SARS-CoV-2.The term "epitope" or "antigenic epitope" includes any molecule, structure, amino acid sequence, or protein determinant that is recognized and specifically bound WO 2021/226560 PCT/US2021/031442 by a cognate binding molecule, such as an immunoglobulin, or other binding molecule, domain, or protein. Epitopic determinants generally contain chemically active surface groupings of molecules, such as amino acids or sugar side chains, and can have specific three-dimensional structural characteristics, as well as specific charge characteristics. Where an antigen is or comprises a peptide or protein, the epitope can be comprised of consecutive amino acids (e.g., a linear epitope), or can be comprised of amino acids from different parts or regions of the protein that are brought into proximity by protein folding (e.g., a discontinuous or conformational epitope), or non-contiguous amino acids that are in close proximity irrespective of protein folding.

Antibodies, Antigen-Binding Fragments, and Compositions In one aspect, the present disclosure provides an isolated antibody, or an antigen-binding fragment thereof, that comprises a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, and is capable of binding to a surface glycoprotein of SARS-CoV-2. In certain embodiments, the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SAKS- C0V-2 expressed on a cell surface of a host cell and/or on a SARS-CoV-2 virion.In certain embodiments, an antibody or antigen-binding fragment of the present disclosure associates with or unites with a SARS-CoV-2 surface glycoprotein epitope or antigen comprising the epitope, while not significantly associating or uniting with any other molecules or components in a sample.In certain embodiments, an antibody or antigen-binding fragment of the present disclosure associates with or unites (e.g., binds) to a SARS-CoV-2 surface glycoprotein epitope, and can also associate with or unite with an epitope from another coronavirus (e.g., SARS-CoV-1) present in the sample, but not significantly associating or uniting with any other molecules or components in the sample. In other words, in certain embodiments, an antibody or antigen binding fragment of the present disclosure is cross-reactive for SARS-CoV-2 and one or more additional coronavirus.In certain embodiments, an antibody or antigen-binding fragment of the present disclosure specifically binds to a SARS-CoV-2 surface glycoprotein. As used herein, WO 2021/226560 PCT/US2021/031442 "specifically binds" refers to an association or union of an antibody or antigen-binding fragment to an antigen with an affinity or Ka (i.e., an equilibrium association constant of a particular binding interaction with units of 1/M) equal to or greater than 105 M1־ (which equals the ratio of the on-rate [KOn] to the off rate [KOff] for this association reaction), while not significantly associating or uniting with any other molecules or components in a sample. Alternatively, affinity may be defined as an equilibrium dissociation constant (Kd) of a particular binding interaction with units of M (e.g., 105־ M to 1013־ M). Antibodies may be classified as "high-affinity" antibodies or as "low- affinity" antibodies. "High-affinity" antibodies refer to those antibodies having a Ka of at least 107M1־, at least 108 M1־, at least 109 M1־, at least 1010 M1־, at least 1011 M1־, at least 1012M1־, or at least 1013 M1־. "Low-affinity" antibodies refer to those antibodies having a Ka of up to 107M1־, up to 106 M1־, up to 105 M1־. Alternatively, affinity may be defined as an equilibrium dissociation constant (Kd) of a particular binding interaction with units of M (e.g., 105־ M to 1013־ M).A variety of assays are known for identifying antibodies of the present disclosure that bind a particular target, as well as determining binding domain or binding protein affinities, such as Western blot, ELISA (e.g., direct, indirect, or sandwich), analytical ultracentrifugation, spectroscopy, and surface plasmon resonance (Biacore®) analysis (see, e.g, Scatchard etal., Ann. N.Y. Acad. Sci. 57:660, 1949; Wilson, Science 295:2103, 2002; Wolff etal.. Cancer Res. 53:2560, 1993; and U.S. Patent Nos. 5,283,173, 5,468,614, or the equivalent). Assays for assessing affinity or apparent affinity or relative affinity are also known.In certain examples, binding can be determined by recombinantly expressing a SARS-CoV-2 antigen in a host cell (e.g., by transfection) and immunostaining the (e.g., fixed, or fixed and permeabilized) host cell with antibody and analyzing binding by flow cytometery (e.g., using a ZES Cell Analyzer (BioRad®) and FlowJo software (TreeStar). In some embodiments, positive binding can be defined by differential staining by antibody of SARS-CoV-2 -expressing cells versus control (e.g., mock) cells.In some embodiments an antibody or antigen-binding fragment of the present disclosure binds to SARS-CoV-2 S protein, as measured using biolayer interferometry.

WO 2021/226560 PCT/US2021/031442 In certain embodiments, an antibody or antigen-binding fragment of the present disclosure binds to SARS-CoV-2 S protein with a Kd of less than about 4.5xl0 9־ M, less than about 5xl0 9־ M, less than about 1x10-10 M, less than about 5xl0 10־ M, less than about 1x10-11 M, less than about 5xl0 ־n M, less than about 1x10-12 M, or less than about 5xl0 12־ M. In some embodiments, an antibody or antigen-binding fragment of the present disclosure binds to SARS-CoV-2 S protein RBD with a Kd of less than about 4.5xl0 9־ M, less than about 5xl0 9־ M, less than about 1x10-10 M, less than about 5xl0 10־ M, less than about 1x10-11 M, less than about 5xl0 ־n M, less than about 1x10-M, or less than about 5xl0 12־ M.Certain characteristics of presently disclosed antibodies or antigen-binding fragments may be described using IC50 or EC50 values. In certain embodiments, the IC50 is the concentration of a composition (e.g., antibody) that results in half-maximal inhibition of the indicated biological or biochemical function, activity, or response. In certain embodiments, the EC50 is the concentration of a composition that provides the half-maximal response in the assay. In some embodiments, e.g., for describing the ability of a presently disclosed antibody or antigen-binding fragment to neutralize infection by SARS-CoV-2, IC50 and EC50 are used interchangeably.In certain embodiments, an antibody of the present disclosure is capable of neutralizing infection by SARS-CoV-2. As used herein, a "neutralizing antibody" is one that can neutralize, i.e., prevent, inhibit, reduce, impede, or interfere with, the ability of a pathogen to initiate and/or perpetuate an infection in a host. The terms "neutralizing antibody" and "an antibody that neutralizes" or "antibodies that neutralize" are used interchangeably herein. In any of the presently disclosed embodiments, the antibody or antigen-binding fragment is capable of preventing and/or neutralizing a SARS-CoV-2 infection in an in vitro model of infection and/or in an in vivo animal model of infection (e.g., using a Syrian hamster model with intranasal delivery of SARS-CoV-2) and/or in a human.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing a SARS-CoV-2 infection or infection by a virus pseudotyped with SARS-CoV-2 S protein with an IC50 of about 16 to about 20 ug/ml.

WO 2021/226560 PCT/US2021/031442 In some embodiments, an antibody or antigen-binding fragment is capable of neutralizing a SARS-CoV-2 infection, or a virus pseudotyped with SARS-CoV-2 S protein, with an IC50 of about 3 to about 4 ug/ml. In any of the presently disclosed embodiments, an antibody or antigen-binding fragment is capable of neutralizing a SARS-CoV-2 infection, or a virus pseudotyped with SARS-CoV-2 S protein, with an IC50, an IC80, and/or an IC90 as shown in Table 4.In some embodiments, an antibody or antigen-binding fragment, or a composition comprising two or more antibodies or antigen-binding fragments, of the present disclosure is capable of neutralizing a SARS-CoV-2 infection, or a virus pseudotyped with SARS-CoV-2 S protein, with an IC50 of about 0.8 to about 0.ug/ml. In some embodiments, an antibody or antigen-binding fragment, or a composition comprising two or more antibodies or antigen-binding fragments, of the present disclosure is capable of neutralizing a SARS-CoV-2 infection, or a virus pseudotyped with SARS-CoV-2 S protein, with an IC50 of about 0.5 to about 0.ug/ml. In some embodiments, an antibody or antigen-binding fragment, or a composition comprising two or more antibodies or antigen-binding fragments, of the present disclosure is capable of neutralizing a SARS-CoV-2 infection, or a virus pseudotyped with SARS-CoV-2, with an IC50 of about 0.1 to about 0.2 ug/ml.In certain embodiments, the antibody or antigen-binding fragment (i) recognizes an epitope in the ACE2 receptor binding motif (RBM, SEQ ID NO. :5) of SARS-CoV- 2; (ii) is capable of blocking an interaction between SARS-CoV-2 and human ACE(z.e. partially or fully blocking the interaction, via binding to SARS-CoV-2); (ii) is capable of binding to SARS-CoV-2 S protein; (iv) recognizes an epitope that is conserved in the ACE2 RBM of SARS-CoV-2 and in an ACE2 RBM of SARS-CoV-1; (v) is cross-reactive against SARS-CoV-2 and SARS-CoV-1; (vi) recognizes an epitope in the SARS-CoV-2 surface glycoprotein that is not in the ACE2 RBM; or (vii) any combination of (i)-(vii).Terms understood by those in the art of antibody technology are each given the meaning acquired in the art, unless expressly defined differently herein. For example, the term "antibody" refers to an intact antibody comprising at least two heavy (H) WO 2021/226560 PCT/US2021/031442 chains and two light (L) chains inter-connected by disulfide bonds, as well as any antigen-binding portion or fragment of an intact antibody that has or retains the ability to bind to the antigen target molecule recognized by the intact antibody, such as an scFv, Fab, or Fab'2 fragment. Thus, the term "antibody" herein is used in the broadest sense and includes polyclonal and monoclonal antibodies, including intact antibodies and functional (antigen-binding) antibody fragments thereof, including fragment antigen binding (Fab) fragments, F(ab')2 fragments, Fab' fragments, Fv fragments, recombinant IgG (rIgG) fragments, single chain antibody fragments, including single chain variable fragments (scFv), and single domain antibodies (e.g., sdAb, sdFv, nanobody) fragments. The term encompasses genetically engineered and/or otherwise modified forms of immunoglobulins, such as intrabodies, peptibodies, chimeric antibodies, fully human antibodies, humanized antibodies, and heteroconjugate antibodies, multi specific, e.g., bispecific antibodies, diabodies, triabodies, tetrabodies, tandem di-scFv, and tandem tri-scFv. Unless otherwise stated, the term "antibody" should be understood to encompass functional antibody fragments thereof. The term also encompasses intact or full-length antibodies, including antibodies of any class or sub-class, including IgG and sub-classes thereof (IgG1, IgG2, IgG3, IgG4), IgM, IgE, IgA, and IgD.The terms "Vl" or "VL" and " Vh" or "VH" refer to the variable binding region from an antibody light chain and an antibody heavy chain, respectively. In certain embodiments, a VL is a kappa (k) class (also "VK" herein). In certain embodiments, a VL is a lambda (X) class. The variable binding regions comprise discrete, well-defined sub-regions known as "complementarity determining regions" (CDRs) and "framework regions" (FRs). The terms "complementarity determining region," and "CDR," are synonymous with "hypervariable region" or "HVR," and refer to sequences of amino acids within antibody variable regions, which, in general, together confer the antigen specificity and/or binding affinity of the antibody, wherein consecutive CDRs (i.e., CDR1 and CDR2, CDR2 and CDR3) are separated from one another in primary structure by a framework region. There are three CDRs in each variable region (HCDR1, HCDR2, HCDR3; LCDR1, LCDR2, LCDR3; also referred to as CDRHs and WO 2021/226560 PCT/US2021/031442 CDRLS, respectively). In certain embodiments, an antibody VH comprises four FRs and three CDRs as follows: FR1-HCDR1-FR2-HCDR2-FR3-HCDR3-FR4; and an antibody VL comprises four FRs and three CDRs as follows: FR1-LCDR1-FR2- LCDR2-FR3-LCDR3-FR4. In general, the VH and the VL together form the antigen- binding site through their respective CDRs.As used herein, a "variant" of a CDR refers to a functional variant of a CDR sequence having up to 1-3 amino acid substitutions (e.g., conservative or non- conservative substitutions), deletions, or combinations thereof.Numbering of CDR and framework regions may be according to any known method or scheme, such as the Rabat, Chothia, EU, IMGT, and AHo numbering schemes (see, e.g., Rabat etal., "Sequences of Proteins of Immunological Interest, US Dept. Health and Human Services, Public Health Service National Institutes of Health, 1991, 5th ed.; Chothia and Lesk, J. Mol. Biol. 796:901-917 (1987)); Lefranc et al., Dev. Comp. Immunol. 27:55, 2003; Honegger and Pliickthun, J. Mol. Bio. 309:651-6(2001)). Equivalent residue positions can be annotated and for different molecules to be compared using Antigen receptor Numbering And Receptor Classification (ANARCI) software tool (2016, Bioinformatics 15:298-300). Accordingly, identification of CDRs of an exemplary variable domain (VH or VL) sequence as provided herein according to one numbering scheme is not exclusive of an antibody comprising CDRs of the same variable domain as determined using a different numbering scheme. In certain embodiments, an antibody or antigen-binding fragment is provided that comprises CDRs of a VH sequence according to any one of SEQ ID NOs.: 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 254, 264, 274, 284, 298, 312, 322, 332, 350,351, 353, 359, 361, 363, 365, 367, 368, 369, 379, 389, 399, 409, 419, 429, 434, 444,454, 464, 474, 484, 494, 504, 514, 524, 534, 544, 554, 564, 574, 584, 594, 604, 614,624, 626, 628, 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658,660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 692, 740, 741, 742,743, 748, 749, 750, 752, 754, 756, 758, 759, 761, 762, and 764 and of a VL sequence according to any one of SEQ IDNOs.: 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133 WO 2021/226560 PCT/US2021/031442 143, 154, 157, 168, 194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278,288, 294, 296, 302, 308, 310, 316, 326, 336, 355, 357, 373, 383, 393, 403, 413, 423,438, 448, 458, 468, 478, 488, 498, 508, 518, 528, 538, 548, 558, 568, 578, 588, 598,608, 618, 686, 696, 738, 744, and 746, as determined using any known CDR numberingmethod, including the Kabat, Chothia, EU, IMGT, Martin (Enhanced Chothia), Contact, and AHo numbering methods. In certain embodiments, CDRs are according to the IMGT numbering method. In certain embodiments, CDRs are according to the antibody numbering method developed by the Chemical Computing Group (CCG); e.g., using Molecular Operating Environment (MOE) software (www.chemcomp.com ).In certain embodiments, an antibody or an antigen-binding fragment is provided that comprises a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein: (i) the CDRH1 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 23, 33, 43, 53, 63, 75, 85, 97, 107,120, 130, 140, 147, 160, 170, 174, 183, 190, 199, 209, 219, 229, 241, 255, 265, 275,285, 299, 313, 323, 333, 370, 380, 390, 400, 410, 420, 430, 435, 445, 455, 465, 475,485, 495, 505, 515, 525, 535, 545, 555, 565, 575, 585, 595, 605, 615, 631, 693, 740,741, 742, and 743, or a sequence variant thereof comprising one, two, or three acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; (ii) the CDRHcomprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 24, 34, 44, 54, 64, 76, 86, 98, 108, 121, 131, 141, 148, 151, 161, 171, 184, 200, 210, 220, 230, 242, 256, 266, 276, 286, 300, 314, 324, 334, 352, 360, 362, 364, 366,371, 381, 391, 401, 411, 421, 431, 436, 446, 456, 466, 476, 486, 496, 506, 516, 526,536, 546, 556, 566, 576, 586, 596, 606, 616, 625, 632, 635, 637, 639, 641, 643, 645,647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671, 673, 675, 677, 679,681, 683, 685, and 694, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; (iii) the CDRH3 comprises or consists of the amino acid sequence according to any one of WO 2021/226560 PCT/US2021/031442 SEQ IDNOs.: 25, 35, 45, 55, 65, 77, 87, 99, 109, 122, 132, 142, 149, 162, 164, 165,172, 176, 177, 179, 180, 185, 187, 188, 201, 211, 221, 231, 243, 257, 267, 277, 287,301, 315, 325, 335, 354, 372, 382, 392, 402, 412, 422, 432, 437, 447, 457, 467, 477,487, 497, 507, 517, 527, 537, 547, 557, 567, 577, 587, 597, 607, 617, 627, 633, 695,751, 753, 755, 757, 760, 763, 765, and 766, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; (iv) the CDRL1 comprises or consists of the amino acid sequence according to any one of SEQ IDNOs.: 27, 37, 47, 57, 67, 79, 89, 101, 111, 124, 134, 144, 152, 155, 156, 158, 159, 166, 181, 192, 203, 213, 223, 233, 245, 259, 269, 279,289, 303, 317, 327, 337, 356, 374, 384, 394, 404, 414, 424, 439, 449, 459, 469, 479,489, 499, 509, 519, 529, 539, 549, 559, 569, 579, 589, 599, 609, 619, 687, and 697, or asequence variant thereof comprising one, two, or three amino acid substitutions, one ormore of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; (v) the CDRL2 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 28, 38, 48, 58, 68, 80, 90, 102, 112, 125, 135, 145, 153, 167, 182, 193, 204, 214, 224, 234, 246, 260, 270, 280, 290, 304, 318, 328, 338, 375, 385, 395, 405, 415, 425, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 688, and 698, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; and/or (vi) the CDRL3 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 29, 39, 49,59, 69, 81, 91, 103, 113, 126, 136, 146, 169, 195, 197, 205, 215, 225, 235, 247, 261,271, 281, 291, 305, 319, 329, 339, 358, 376, 386, 396, 406, 416, 426, 441, 451, 461,471, 481, 491, 501, 511, 521, 531, 541, 551, 561, 571, 581, 591, 601, 611, 621, 689,699, 745, and 747, or a sequence variant thereof comprising having one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid, WO 2021/226560 PCT/US2021/031442 wherein the antibody or antigen binding fragment is capable of binding to a surface glycoprotein of SARS-C0V-2 expressed on a cell surface of a host cell.In any of the presently disclosed embodiments, the antibody or antigen-binding fragment is capable of preventing and/or neutralizing a SARS-CoV-2 infection in an in vitro model of infection and/or in an in vivo animal model of infection and/or in a human.In any of the presently disclosed embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs.: (i) 23-25 and 27-29, respectively; (ii) 33-and 37-39, respectively; (iii) 43-45 and 47-49, respectively; (iv) 53-55 and 57-59, respectively; (v) 63-65 and 67-69, respectively; (vi) 75-77 and 79-81, respectively; (vii) 85-87 and 89-91, respectively; (viii) 97-99 and 101-103, respectively; (ix) 107-109 and 111-113, respectively; (x) 120-122 and 124-126, respectively; (xi) 130-132 and 134- 136, respectively; (xii) 23 or 147, any one of 24, 148 or 151, 25 or 149, any one of 27, 152, 155, 156, 158, or 159, 28 or 153, and 29, respectively; (xiii) 43 or 160, 44 or 161, any one of 45, 162, 164, or 165, 47 or 166, 48 or 167, and 49 or 169, respectively; (xiv) any one of 130, 170, or 174, 130, 131, 132, 134 or 181, 135 or 182, and 136, respectively; (xv) any one of 53, 183, or 190, 54 or 184, any one of 55, 185, 187, or 188, 57 or 192, 58 or 193, and any one of 59, 195, or 197, respectively; (xvi) 199-2and 203-205, respectively; (xvii) 209-211 and 213-215, respectively; (xviii) 219-2and 223-225, respectively; (xix) 229-231 and 233-235, respectively; (xx) 241-243 and 245-247, respectively; (xxi) 255-257 and 259-261, respectively; (xxii) 265-267 and 269-271, respectively; (xxiii) 275-277 and 279-281, respectively; (xxiv) 285-287 289- 291, respectively, (xxv) 299-301 and 303-305, respectively; (xxvi) 313-315 and 317- 319, respectively; (xxvii) 323-325 and 327-329, respectively; (xxviii) 333-335 and 337- 339, respectively; (xxix) 229, 230 or 352, 231 or 354, and 233 or 356, 234, and 235 or 358, respectively; (xxx) 313, any one of 314, 360, 362, 364, or 366, 315, and 317-319, respectively; (xxxi) 370-372 and 374-376, respectively; (xxxii) 380-382 and 384-386, respectively; (xxxiii) 390-392 and 394-396, respectively; (xxxiv) 400-402 and 404-406, respectively; (xxxv) 410-412 and 414-416, respectively; (xxxvi) 420-422 and 424-426, WO 2021/226560 PCT/US2021/031442 respectively; (xxxvn) 435-437 and 439-441, respectively; (xxxvm) 445-447 and 449- 451, respectively; (xxxix) 455-457 and 459-461, respectively; (xxxx) 465-467 and 469- 471, respectively; (xxxxi) 475-477 and 479-481, respectively; (xxxxii) 485-487 and 489-491, respectively; (xxxxiii) 494-497 and 499-501, respectively; (xxxxiv) 505-5and 509-511, respectively; (xxxxv) 515-517 and 519-521, respectively; (xxxxvi) 525- 527 and 529-531, respectively; (xxxxvii) 535-537 and 539-541, respectively; (xxxxviii) 545-547 and 549-551, respectively; (xxxxix) 555-557 and 559-561, respectively;(xxxxx) 565-567 and 569-571, respectively; (xxxxxi) 575-577 and 579-581, respectively; (xxxxxii) 585, 586 or 625, 587 or 627, and 589-591, respectively; (xxxxxiii) 595-597 and 599-601, respectively; (xxxxxiv) 605-607 and 609-611, respectively; (xxxxxv) 615-617 and 619-621, respectively, (xxxxxvi) 631, 632 or 6or 637 or 639 or 641 or 643 or 645 or 647 or 649 or 651 or 653 or 655 or 657 or 659 or 661 or 663 or 665 or 667 or 669 or 671 or 673 or 675 or 677 or 679 or 681 or 683 or 685, 633, and 697-699, respectively; (xxxxxvii) 693-695 and 697-699, respectively; (xxxxxviii) 400, 401 and any one of 751, 753, 755, 757, or 760 and 404, 405, and any one of 745 or 747, respectively; (xxxxxxix) 585, 586, and 762 or 764 and 589-591, respectively; or (xxxxxxx) 400, 401, 766, and 404-406, respectively.In any of the presently disclosed embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRLI, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :631, 632 or 635 or 637 or 639 or 641 or 6or 645 or 647 or 649 or 651 or 653 or 655 or 657 or 659 or 661 or 663 or 665 or 667 or 669 or 671 or 673 or 675 or 677 or 679 or 681 or 683 or 685, 633, and 697-699, respectively. In any of the presently disclosed embodiments, the antibody or antigen- binding fragment comprises CDRH1, CDRH2, CDRH3, CDRLI, CDRL2, and CDRLamino acid sequences according to SEQ ID NOs. :693-695 and 697-699, respectively.In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRLI, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400-402, and 404-406, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRLI, CDRL2, and CDRL3 amino acid sequences according to SEQ ID WO 2021/226560 PCT/US2021/031442 NOs. :400, 401, 766, and 404-406, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400-402, 404, 405, and 745, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400, 401, 766, 404, 405, and 745, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400-402, 404, 405, and 747, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400, 401, 766, 404, 405, and 747, respectively. In certain embodiments, the antibody or antigen- binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRLamino acid sequences according to SEQ ID NOs. :400, 401, 751, and 404-406, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400, 401, 753, and 404-406, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400, 401, 755, and 404-406, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400, 401, 757, and 404-406, respectively. In certain embodiments, the antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs. :400, 401, 760, and 404-406, respectively.In certain embodiments, an antibody or an antigen-binding fragment of the present disclosure comprises a CDRH1, a CDRH2, a CDRH3, a CDRL1, a CDRL2, and a CDRL3, wherein each CDR is independently selected from a corresponding CDR of SARS-C0V-2 S2X16-V1 mAb, SARS-C0V-2 S2X16-v2 mAb, SARS-C0V-2 S2X16-vmAb, SARS-C0V-2 S2X16-v4 mAb, SARS-C0V-2 S2X16-v5 mAb, SARS-C0V-2 WO 2021/226560 PCT/US2021/031442 S2X16-V6 mAb, SARS-CoV-2 S2X16-V7 mAb, SARS-CoV-2 S2X16-v8 mA,b SARS- C0V-2 S2X28-V1 mAb, SARS-CoV-2 S2X30-vl mAb, SARS-CoV-2 S2X30-v2 mAb, SARS-CoV-2 S2X30-v3 mAb, SARS-CoV-2 S2X30-v4 mAb, SARS-CoV-2 S2X30-vmAb, SARS-CoV-2 S2X30-v6 mAb, SARS-CoV-2 S2X47-vl mAb, SARS-CoV-S2X47-v2 mAb, SARS-CoV-2 S2X47-v3 mAb, SARS-CoV-2 S2X47-v4 mAb, SARS- C0V-2 S2X47-v5 mAb, SARS-CoV-2 S2X47-v6 mAb, SARS-CoV-2 S2X47-v7 mAb, SARS-CoV-2 S2X47-v8 mAb, SARS-CoV-2 S2X47-v9 mAb, SARS-CoV-2 S2X55-vl mAb, SARS-CoV-2 S2X55-v2 mAb, SARS-CoV-2 S2X56-vl mAb, SARS-CoV-S2X58-V1 mAb, SARS-CoV-2 S2X58-v2 mAb, SARS-CoV-2 S2X71-vl mAb SARS- C0V-2 S2X76-V1 mAb, SARS-CoV-2 S2X76-v2 mAb, SARS-CoV-2 S2X76-v3 mAb, SARS-CoV-2 S2X76-v4 mAb, SARS-CoV-2 S2Xll-vl mAb, or SARS-CoV-2 S2X35- vl mAb, SARS-CoV-2 S2X35-v2 mAb, SARS-CoV-2 S2X35-v3 mAb, SARS-CoV-S2X35-v4 mAb, SARS-CoV-2 S2X35-v5 mAb, SARS-CoV-2 S2X35-v6 mAb, SARS- C0V-2 S2X35-v7 mAb, SARS-CoV-2 S2X35-v8 mAb, SARS-CoV-2 S2H30-vl mAb, SARS-CoV-2 S2H37-V1 mAb, SARS-CoV-2 S2H40-vl mAb, SARS-CoV-2 S2H58-vl mAb, SARS-CoV-2 S2H58-v2 mAb, SARS-CoV-2 S2H58-v3 mAb, SARS-CoV-S2H58-v4 mAb, SARS-CoV-2 S2H58-v5 mAb, SARS-CoV-2 S2H58-v6 mAb, SARS- C0V-2 S2H58-v7 mAb, SARS-CoV-2 S2H62-vl mAb, SARS-CoV-2 S2H62-v2 mAb, SARS-CoV-2 S2H62-v3 mAb, SARS-CoV-2 S2H66-vl mAb, SARS-CoV-2 S2H66-vmAb, SARS-CoV-2 S2H66-v3 mAb, SARS-CoV-2 S2H70-vl mAb, SARS-CoV-S2H71-V1 mAb, SARS-CoV-2 S2H73-vl mAb, SARS-CoV-2 S2N12-vl mAb, SARS- C0V-2 S2N12-v2 mAb, SARS-CoV-2 S2N12-v3 mAb, SARS-CoV-2 S2N22-vl mAb, SARS-CoV-2 S2N22-v2 mAb, SARS-CoV-2 S2N22-v3 mAb, SARS-CoV-2 S2N22-vmAb, SARS-CoV-2 S2N22-v5 mAb, SARS-CoV-2 S2N22-v6 mAb, SARS-CoV-S2N22-v7 mAb, SARS-CoV-2 S2N25-vl mAb, SARS-CoV-2 S2N28-vl mAb, SARS- C0V-2 S2E6-V1 mAb, SARS-CoV-2 S2E7-vl mAb, SARS-CoV-2 S2E9-vl mAb, SARS-CoV-2 S2E12-V1 mAb, SARS-CoV-2 S2E12-v2 mAb, SARS-CoV-2 S2E13-vl mAb, SARS-CoV-2 S2E14-vl mAb, SARS-CoV-2 S2K4-vl mAb, SARS-CoV-S2X193-V1 mAb, SARS-CoV-2 S2X195-vl mAb, SARS-CoV-2 S2X219-vl mAb, SARS-CoV-2 S2X244-V1 mAb, SARS-CoV-2 S2X246-vl mAb, SARS-CoV-2 WO 2021/226560 PCT/US2021/031442 S2X256-V1 mAb, SARS-CoV-2 S2X269-vl mAb, SARS-CoV-2 S2X278־vl mAb, SARS-CoV-2 S2M7-V1 mAb, SARS-CoV-2 S2Mll-vl mAb, SARS-CoV-2 S2M16-vl mAb, SARS-CoV-2 S2M28-V1 mAb, SARS-CoV-2 S2L49-vl mAb, SARS C0V-S2D65-V1 mAb, SARS C0V-2 S2D97-vl mAb, SARS C0V-2 S2D106-vl mAb, SARS C0V-2 S2X149-V1 mAb, SARS C0V-2 S2X179-vl mAb, SARS-CoV-2 S2H1mAb, Antibody 409_l l_l_v2, Antibody 409_l l_l_v3, Antibody 409_l l_l_v4, Antibody 409_ll_l_v5, Antibody 409_ll_l_v6, Antibody 409_ll_2_vl, Antibody 409_l l_2_v2, Antibody 409_l l_2_v3, Antibody 409_l l_2_v4, Antibody 409_l l_2_v5, Antibody 409_l l_2_v6, Antibody 409_l l_2_v7, Antibody 409_ll_2_v8, Antibody 409_ll_2_v9, Antibody 409_ll_2_vl0, Antibody 409_l l_2_vl 1, Antibody 409_l l_2_vl2, Antibody 409_l l_2_vl3, Antibody 409_ll_2_vl4, Antibody 409_ll_2_vl5, Antibody 409_ll_2_vl6, Antibody 409_ll_2_vl7, Antibody 409_l l_2_vl8, Antibody 409_ll_2_vl9, Antibody 409_l l_2_v20, Antibody 409_l l_2_v21, Antibody Antibody 409_l l_2_v22, Antibody 409_l l_2_v23, Antibody 409_l l_2_v24, Antibody 409_l l_2_v25, Antibody 409_l l_2_v26, Antibody 409_l l_2_v27, Antibody 409_l l_3_vl, Antibody 409_l l_4_v2, Antibody 409_l l_4_v3, Antibody 409_l l_4_v4, Antibody 409_l l_4_v5, Antibody 409_l l_4_v6, Antibody 409_l l_4_v7, Antibody 409_ll_4_v8, Antibody 409_ll_4_v9, Antibody 409_l l_4_vl0, Antibody 409_l l_4_vl 1, Antibody 409_l l_4_vl2, or Antibody 409_l l_4_vl3, as provided in Table 2. That is, all combinations of CDRs from SARS-CoV-2 mAbs and the variant sequences thereof provided in Table 2 are contemplated.During antibody development, DNA in the germline variable (V), joining (J), and diversity (D) gene loci may be rearranged and insertions and/or deletions of nucleotides in the coding sequence may occur. Somatic mutations may be encoded by the resultant sequence, and can be identified by reference to a corresponding known germline sequence. In some contexts, somatic mutations that are not critical to a desired property of the antibody (e.g., binding to a SARS-CoV-2 antigen), or that confer an undesirable property upon the antibody (e.g., an increased risk of immunogenicity in a subject administered the antibody), or both, may be replaced by WO 2021/226560 PCT/US2021/031442 the corresponding germhne-encoded amino acid, or by a different amino acid, so that a desirable property of the antibody is improved or maintained and the undesirable property of the antibody is reduced or abrogated. Thus, in some embodiments, the antibody or antigen-binding fragment of the present disclosure comprises at least one more germline-encoded amino acid in a variable region as compared to a parent antibody or antigen-binding fragment, provided that the parent antibody or antigen binding fragment comprises one or more somatic mutations. Variable region and CDR amino acid sequences of anti-SARS-CoV-2 antibodies of the present disclosure are provided in Table 2 herein.Exemplary antibodies of the present disclosure include antibody S2E12 and engineered variants thereof. Engineered S2E12 variants include "Antibody 409_l l_4_v2", "Antibody 409_l l_4_v3", "Antibody 409_l l_4_v4", "Antibody 409_l l_4_v5", "Antibody 409_l l_4_v6", "Antibody 409_l l_4_v7", "Antibody 409_l l_4_v8", "Antibody 409_l l_4_v9", "Antibody 409_l l_4_vl0", "Antibody 409_ll_4_vl 1", "Antibody 409_ll_4_vl2", "Antibody 409_ll_4_vl3". In particular embodiments, an antibody or antigen-binding fragment comprises a CDRH1, a CDRH2, a CDRH3, a CDRLI, a CDRL2, and/or a CDRL3 selected from any of the CDRH1, CDRH2, CDRH3, CDRLI, CDRL2, and CDRL3 amino acid sequences (respectively) provided in Table 1. Table 1 also provides amino acid sequences that comprise S2E12 CDRH3 sequences and two amino acids (Ala-Ser) that are immediately N-terminal to CDRH3 in S2E12.In some embodiments, an antibody or antigen-binding fragment comprises: a CDRH1, a CDRH2, and/or a CDRH3 of the VH amino acid sequence set forth in any one of SEQ ID NOs.:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761; and a CDRLI, a CDRL2, and/or a CDRL3 of the VL amino acid sequence set forth in any one of SEQ ID NOs.:403, 738, 744, and 746 (i.e., according to any CDR numbering or determination method known in the art, such as IMGT, Rabat, Chothia, AHo, North, Contact, CCG, EU, or Martin (Enhanced Chothia)). For example, in some embodiments, the antibody or antigen-binding fragment comprises the CDRH1, the CDRH2, and/or the CDRH3 of the VH amino acid sequence set forth in SEQ ID WO 2021/226560 PCT/US2021/031442 NO. :399, and the CDRL1, the CDRL2, and/or the CDRL3 of the VL amino acid sequence set forth in SEQ ID NO.:738, wherein the CDRs are according to IMGT. As another non-limiting example, in some embodiments, the antibody or antigen-binding fragment comprises the CDRH1, the CDRH2, and the CDRH3 of the VH amino acid sequence set forth in SEQ ID NO. :399, and the CDRL1, the CDRL2, and the CDRL3 of the VL amino acid sequence set forth in SEQ ID NO.:738, wherein the CDRs are according to IMGT.In further embodments, the antibody or antigen-binding fragment comprises a VH having at least 85% identity (e.g., 85%, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100%) identity to a VH amino acid sequence provided in Table 1 and/or a VL having at least 85% identity (e.g.., 85%, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100%) identity to a VL amino acid sequence provided in Table 1. In still further embodments, the antibody or antigen-binding fragment comprises a VH having at least 90% identity identity to a VH amino acid sequence provided in Table 1 and/or a VL having at least 90% identity to a VL amino acid sequence provided in Table 1. In still further embodments, the antibody or antigen-binding fragment comprises a VH having at least 95% identity identity to a VH amino acid sequence provided in Table and/or a VL having at least 95% identity to a VL amino acid sequence provided in Table 1. In still further embodments, the antibody or antigen-binding fragment comprises a VH having at least 99% identity identity to a VH amino acid sequence provided in Table 1 and/or a VL having at least 99% identity to a VL amino acid sequence provided in Table 1. In some embodiments, the antibody or antigen-binding fragment comprises a VH amino acid sequence selected from the VH amino acid sequences provided in Table 1 and a VL amino acid sequence selected from the VL amino acid sequence provided in Table 1. In some embodiments, S2E12 antibodies comprise a kappa light chain, e.g., klm3, IGKC*01.

Table 1. CDR (IMGT) and Variable Region Amino Acid Sequences of Certain S2E12 Antibodies CDRHI GFTFTSSA (SEQ ID NO. :400) WO 2021/226560 PCT/US2021/031442 CDRH2 IVVGSGNT (SEQ ID NO. :401)CDRH3 PYCSGGSCSDGFDI (SEQ ID NO. :766) PYCSGGSCSDAFDI (SEQ ID NO. :769) PYSSGGSSSDGFDI (SEQ ID NO. :770) PYPSGGSPSDGFDI (SEQ ID NO. :771) PYASGGSASDGFDI (SEQ ID NO.:772) PYCSGGSCSEGFDI (SEQ ID NO.:773)CDRH3 and N- ASPYCSGGSCSDGFDI (SEQ ID NO. :402)terminal adjacent ASPYCSGGSCSDAFDI (SEQ ID NO. :751)alanine ASPYSSGGSSSDGFDI (SEQ ID NO. :753)serine ASPYPSGGSPSDGFDI (SEQ ID NO. :755)acids ASPYASGGSASDGFDI (SEQ ID NO.:757)ASPYCSGGSCSEGFDI (SEQ ID NO.:760) VH QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAYM ELSSLRSEDTAVYYCASPYCSGGSCSDGFDIWGQGTMVTVSS (SEQ ID NO. :399) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGFIVVGSGNTNYAQKFHERVTITRDMSTSTAYME LSSLRSEDTAVYYCASPYCSGGSCSDGFDIWGQGTMVTVSS (SEQ ID NO. :748) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGYTVVGSGNTNYAQKFHERVTITRDMSTSTAYME LSSLRSEDTAVYYCASPYCSGGSCSDGFDIWGQGTMVTVSS (SEQ ID NO. :749) WO 2021/226560 PCT/US2021/031442 QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAYM ELSSLRSEDTAVYYCASPYCSGGSCSDAFDIWGQGTMVTVSS (SEQ ID NO. :750) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAYM ELS SLRSEDT AVYYC ASP YS SGGS S SDGFDIWGQGTMVT VS S (SEQ ID NO. :752) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAYM ELSSLRSEDTAVYYCASPYPSGGSPSDGFDIWGQGTMVTVSS (SEQ ID NO. :754) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAMEL SSLRSEDTAVYYCASPYASGGSASDGFDIWGQGTMVTVSS (SEQ ID NO. :756) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGFIVVGSGNTNYAQKFHERVTITRDMSTSTAYME LSSLRSEDTAVYYCASPYCSGGSCSEGFDIWGQGTMVTVSS (SEQ ID NO. :758) QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAYM ELSSLRSEDTAVYYCASPYCSGGSCSEGFDIWGQGTMVTVSS (SEQ ID NO. :759) WO 2021/226560 PCT/US2021/031442 QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQAR GQRLEWVGAIVVGSGNTNYAQKFHERVTITRDMSTSTAYME LSSLRSEDTAVYYCASPYCSGGSCSDGFDIWGQGTMVTVSS (SEQ ID NO. :761) CDRL1 QSVSSSY (SEQ ID NO. :404) CDRL2 GAS (SEQ ID NO. :405) CDRL3 QQYVGLTGWT (SEQ ID NO. :406)QQYVGLTGFT (SEQ ID NO. :745)QQYVGLTGYT (SEQ ID NO.:747)VL DIVLTQTPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPG QAPRLLIYGAS SRATGIPDRF SGSGSGTDFTLTISRLEPEDF AV YYCQQYVGLTGWTFGQGTKVEIK (SEQ ID NO. :403) DIVLTQTPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPG QAPRALIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAV YYCQQYVGLTGWTFGQGTKVEIK (SEQ ID NO.:738) DIVLTQTPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPG QAPRLLIYGAS SRATGIPDRF SGSGSGTDFTLTISRLEPEDF AV YYCQQYVGLTGFTFGQGTKVEIK (SEQ ID NO.:744) DIVLTQTPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPG QAPRLLIYGAS SRATGIPDRF SGSGSGTDFTLTISRLEPEDF AV YYCQQYVGLTGYTFGQGTKVEIK (SEQ ID NO. :746) In some embodiments, an antibody, or an antigen-binding fragment thereof, comprises a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRH1, CDRH2, CDRH3, WO 2021/226560 PCT/US2021/031442 CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 766, 404, 405, and 406, respectively; (b) SEQ ID NOs. :400, 401, 769, 404, 405, and 406, respectively; (c) SEQ ID NOs. :400, 401, 770, 404, 405, and 406, respectively; (d) SEQ ID NOs. :400, 401, 771, 404, 405, and 406, respectively; (e) SEQ ID NOs. :400, 401, 772, 404, 405, and 406, respectively; (f) SEQ ID NOs. :400, 401, 773, 404, 405, and 406, respectively; (g) SEQ ID NOs.:400, 401, 766, 404, 405, and 745, respectively; (h) SEQ ID NOs. :400, 401, 769, 404, 405, and 745, respectively; (i) SEQ ID NOs. :400, 401, 770, 404, 405, and 745, respectively; (j) SEQ ID NOs. :400, 401, 771, 404, 405, and 745, respectively; (k) SEQ ID NOs. :400, 401, 772, 404, 405, and 745, respectively; (1) SEQ ID NOs.: 400, 401, 773, 405, 405, and 745, respectively; (m) SEQ ID NOs. :400, 401, 766, 404, 405, and 747, respectively; (n) SEQ ID NOs. :400, 401, 769, 404, 405, and 747, respectively; (o) SEQ ID NOs. :400, 401, 770, 404, 405, and 747, respectively; (p) SEQ ID NOs. :400, 401, 771, 404, 405, and 747, respectively; (q) SEQ ID NOs. :400, 401, 772, 404, 405, and 747, respectively; or (r) SEQ ID NOs. :400, 401, 773, 404, 405, and 747, respectively.In further embodiments, the antibody or antigen-binding fragment comprises the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 402, 404, 405, and 406; (b) SEQ ID NOs. :400, 401, 751, 404, 405, and 406; (c) SEQ ID NOs. :400, 401, 753, 404, 405, and 406; (d) SEQ ID NOs. :400, 401, 755, 404, 405, and 406; (e) SEQ ID NOs. :400, 401, 757, 404, 405, and 406; (f) SEQ ID NOs. :400, 401, 760, 404, 405, and 406; (g) SEQ ID NOs. :400, 401, 402, 404, 405, and 745; (h) SEQ ID NOs. :400, 401, 751, 404, 405, and 745; (i) SEQ ID NOs.:400, 401, 753, 404, 405, and 745; (j) SEQ ID NOs. :400, 401, 755, 404, 405, and 745; (k) SEQ ID NOs.:400, 401, 757, 404, 405, and 745; (1) SEQ ID NOs.: 400, 401, 760, 405, 405, and 745; (m) SEQ ID NOs.:400, 401, 402, 404, 405, and 747; (n) SEQ ID NOs. :400, 401, 751, 404, 405, and 747; (o) SEQ ID NOs. :400, 401, 753, 404, 405, and 747; (p) SEQ ID NOs.:400, 401, 755, 404, 405, and 747; (q) SEQ ID NOs. :400, 401, 757, 404, 405, and 747; or (r) SEQ ID NOs. :400, 401, 760, 404, 405, and 747. In each of (a)-(r) above, it will be understood that the first three recited SEQ ID NOs. are VH amino acid sequences, and the latter three recited SEQ ID NOs. are VL amino acid sequences. For example, in (a), SEQ ID NOs. :400, WO 2021/226560 PCT/US2021/031442 401, and 402 are amino acid sequences in VH, and SEQ ID NOs. :404, 405, and 406 are amino acid sequences in VL.Other exemplary antibodies include S2M11, S2D106, S2H58, and engineered variants thereof. In some embodiments, an antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs. :525-527 and 529-531, respectively. In further embodiments, the antibody or antigen-binding fragment comprises a VH having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in SEQ ID NO.:524, and a VL having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in SEQ ID NO.:528. In other embodiments, an antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.: 585, 586, 587, 589, 590, and 591, respectively, or as set forth in SEQ ID NOs. :585, 625, 627, 589, 590, and 591, respectively. In further embodiments, the antibody or antigen-binding fragment comprises a VH having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in any one of SEQ ID NOs. :584, 624, 626, and 628, and a VL having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in SEQ ID NO.:588. In other embodiments, an antibody or antigen-binding fragment comprises CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.: 229, 230, 231, 233, 234, and 235, respectively. In further embodiments, the antibody or antigen-binding fragment comprises a VH having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in any one of SEQ ID NOs. :228, 740, 741, 742, and 743, and a VL having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in SEQ ID NO. :232. In other embodiments, , the antibody or antigen-binding fragment comprises a VH having at least 80%, at least 85%, at least WO 2021/226560 PCT/US2021/031442 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in any one of SEQ ID NOs. :228, 740, 741, 742, and 743, and a VL having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or comprising or consisting of, the amino acid sequence set forth in SEQ ID NO.:238.In certain embodiments, an antibody or antigen-binding fragment comprises an amino acid modification (e.g., a substitution mutation) to remove an undesired risk of oxidation, deamidation, and/or isomerization.Variant antibodies provided herein include those that comprise one or more amino acid alterations in a variable region (e.g., VH, VL, framework or CDR) as compared to a presently disclosed antibody having a specific sequence, wherein the variant antibody is capable of binding to a SARS-CoV-2 antigen.In certain embodiments, the VH comprises or consists of an amino acid sequence having at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence according to any one of SEQ ID NOs.: 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 254,264, 274, 284, 298, 312, 322, 332, 350, 351, 353, 359, 361, 363, 365, 367, 368, 369,379, 389, 399, 409, 419, 429, 434, 444, 454, 464, 474, 484, 494, 504, 514, 524, 534,544, 554, 564, 574, 584, 594, 604, 614, 624, 626, 628, 630, 634, 636, 638, 640, 642,644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676,678, 680, 682, 684, 692, 740, 741, 742, 743, 748, 749, 750, 752, 754, 756, 758, 759,761, 762, and 765, wherein the variation is optionally limited to one or more framework regions and/or the variation comprises one or more substitution to a germline-encoded amino acid; and/or (ii) the VL comprises or consists of an amino acid sequence having at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence according to any one of SEQ ID NOs.: 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133, 143, 154, 157, 168, 194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278, 288, 294, 296, 302, 308, 310, 316, 326, 336, 355, 357, 373, 383, 393, 403, 413, 423, 438, 448, WO 2021/226560 PCT/US2021/031442 458, 468, 478, 488, 498, 508, 518, 528, 538, 548, 558, 568, 578, 588, 598, 608, 618, 686, 696, 738, 744, and 746, wherein the variation is optionally limited to one or more framework regions and/or the variation comprises one or more substitution to a germline-encoded amino acid.In further embodments, the VH has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, and the VL has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in any one of SEQ ID NOs.:403, 738, 744, and 746.In further embodments, the VH has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in any one of SEQ ID NOs.:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, and the VL has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in SEQ ID NO. :403.In further embodments, the VH has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, and the VL has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in SEQ ID NO.:738.In further embodments, the VH has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, and the VL has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, WO 2021/226560 PCT/US2021/031442 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in SEQ ID NO. :744.In further embodments, the VH has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in any one of SEQ ID NOs.:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, and the VL has at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :748 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :748 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :748 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :748 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :749 and the VL comprises or consists of the amino acid WO 2021/226560 PCT/US2021/031442 sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :7and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO.:749 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :749 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :750 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :750 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO.:750 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :750 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :750 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :752 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO.:752 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth WO 2021/226560 PCT/US2021/031442 in SEQ ID NO. :752 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :754 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :754 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :754 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :754 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :756 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :756 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :756 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :756 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :758 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :758 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :758 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino WO 2021/226560 PCT/US2021/031442 acid sequence set forth in SEQ ID NO.:758 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :759 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :759 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :759 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :759 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :761 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :403. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :761 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO.:738. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :761 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :744. In some embodments, the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO. :761 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO. :746.In some embodiments, the VH comprises or consists of any VH amino acid sequence set forth in Table 2, and the VL comprises or consists of any VL amino acid sequence set forth in Table 2. In particular embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.: (i) 22 and 26, respectively; (ii) 32 and 36, respectively; (iii) 42 and 46, respectively; (iv) 52 and 56, respectively; (v) 62 and 66, respectively; (vi) WO 2021/226560 PCT/US2021/031442 and 66, respectively; (vn) 74 and 78, respectively; (vm) 84 and 88, respectively; (ix) and 94, respectively; (x) 96 and 100, respectively; (xi) 106 and 110, respectively; (xii) 119 and 123, respectively; (xiii) 129 and 133, respectively; (xiv) 22 or 150 and 26, 154, or 157, respectively; (xv) 42 or 163 and 46 or 168, respectively; (xvi) any one of 129, 173, 175, or 178 and 133, respectively; (xvii) any one of 52, 186, 189, or 191 and any one of 56, 194, or 196, respectively; (xviii) 198 and 202, respectively; (xix) 208 and 212, respectively; (xx) 218 and 222, respectively; (xxi) 228 and 232 or 238, respectively; (xxii) 240 and any one of 244, 250, or 252, respectively; (xxiii) 254 and 258, respectively; (xxiv) 264 and 268, respectively; (xxv) 274 and 278, respectively; (xxvi) 284 and any one of 288, 294, or 296, respectively; (xxvii) 298 and any one of 302, 308, or 310, respectively; (xxviii) 312 and 316, respectively; (xxix) 322 and 326, respectively; (xxx) 332 and 336, respectively, (xxxi) any one of 228, 350, 351, or 3and any one of 232, 238, 355, or 357, respectively; (xxxii) any one of 312, 359, 361, 363, 365, 367, or 368 and 316, respectively; (xxxiii) 369 and 373, respectively; (xxxiv) 379 and 383, respectively; (xxxv) 389 and 393, respectively; (xxxvi) 399 and 403 or 738, respectively; (xxxvii) 409 and 413, respectively; (xxxviii) 419 and 423, respectively; (xxxix) 434 and 438, respectively; (xxxx) 444 and 448, respectively;(xxxxi) 454 and 458, respectively; (xxxxii) 464 and 468, respectively; (xxxxiii) 474 and 478, respectively; (xxxxiv) 484 and 488, respectively; (xxxxv) 494 and 498, respectively; (xxxxvi) 504 and 508, respectively; (xxxxvii) 514 and 518, respectively; (xxxxviii) 524 and 528, respectively; (xxxxix) 534 and 538, respectively; (xxxxx) 5and 548, respectively; (xxxxxi) 554 and 558, respectively; (xxxxxii) 564 and 568, respectively; (xxxxxiii) 574 and 578, respectively; (xxxxxiv) 584 and 588, respectively; (xxxxxv) 594 and 598, respectively; (xxxxxvi) 604 and 608, respectively; (xxxxxvii) 614 and 618, respectively; (xxxxxviii) 624, 626, or 628 and 588, respectively;(xxxxxix) 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, or 684, and 686, respectively; (xxxxxx) 692 and 696, respectively; (xxxxxxi) any one of 740-743 and 238, respectively, (xxxxxxii) any one of 399, 748, 749, 750, 752, 754, 756, 758, 759 or 7 WO 2021/226560 PCT/US2021/031442 and any one of 403, 744, or 746, respectively; or (xxxxxxm) 762 or 764 and 588, respectively.In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.: 624, 626, or 628 and 588, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.: 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, or 684, and 686, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :692 and 696, respectively.In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.: 399 and 738, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :399 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :399 and 738, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :399 and 744, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :399 and 746, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :748 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :749 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :750 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :752 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :754 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs. :756 and 403, respectively. In certain embodiments, the VH WO 2021/226560 PCT/US2021/031442 and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.:758 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.:759 and 403, respectively. In certain embodiments, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.:761 and 403, respectively.The term "CL" refers to an "immunoglobulin light chain constant region" or a "light chain constant region," i.e., a constant region from an antibody light chain. The term "CH" refers to an "immunoglobulin heavy chain constant region" or a "heavy chain constant region," which is further divisible, depending on the antibody isotype into CHI, CH2, and CH3 (IgA, IgD, IgG), or CHI, CH2, CH3, and CH4 domains (IgE, IgM). The Fc region of an antibody heavy chain is described further herein. In any of the presently disclosed embodiments, an antibody or antigen-binding fragment of the present disclosure comprises any one or more of: a CL, a CHI, a CH2, and a CH3. In certain embodiments, a CL comprises an amino acid sequence having 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to the amino acid sequence of SEQ ID NO. :8 or SEQ ID NO.: 9. In certain embodiments, a CH1-CH2-CH3 (also referred-to as a CH1-CH3) comprises an amino acid sequence having 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to the amino acid sequence of SEQ ID NO. :6 or SEQ ID NO.:7.In certain embodiments, an antibody or antigen-binding fragment of the present disclosure comprises a heavy chain polypeptide comprising or consisting of the amino acid sequence set forth in SEQ ID NO. :767 and a light chain polypeptide comprising or consisting of the amino acid sequence set forth in SEQ ID NO. :768.It will be understood that, for example, production in a mammalian cell line can remove one or more C-terminal lysine of an antibody heavy chain (see, e.g., Liu et al. mAbs 6(5): 1145-1154 (2014)). Accordingly, an antibody or antigen-binding fragment of the present disclosure can comprise a heavy chain, a CH1-CH3, a CH3, or an Fc polypeptide wherein a C-terminal lysine residue is present or is absent; in other words, encompassed are embodiments where the C-terminal residue of a heavy chain, a CHI- CH3, or an Fc polypeptide is not a lysine due to removal of a C-terminal lysine, and WO 2021/226560 PCT/US2021/031442 embodiments where a lysine is the C-terminal residue. In certain embodiments, a composition comprises a plurality of an antibody and/or an antigen-binding fragment of the present disclosure, wherein one or more antibody or antigen-binding fragment does not comprise a lysine residue at the C-terminal end of the heavy chain, CH1-CH3, or Fc polypeptide, and wherein one or more antibody or antigen-binding fragment comprises a lysine residue at the C-terminal end of the heavy chain, CH1-CH3, or Fc polypeptide. In other words, in certain embodiments, a heavy chain can comprise or consist of the amino acid sequence set forth in SEQ ID NO. :767 without the C-terminal lysine. In certain embodiments, a heavy chain or a CH1-CH3 can comprise of the amino acid sequence set forth in SEQ ID NO. :6 or SEQ ID ID NO. :7 without the C-terminal lysine.A "Fab" (fragment antigen binding) is the part of an antibody that binds to antigens and includes the variable region and CHI of the heavy chain linked to the light chain via an inter-chain disulfide bond. Each Fab fragment is monovalent with respect to antigen binding, i.e., it has a single antigen-binding site. Pepsin treatment of an antibody yields a single large F(ab')2 fragment that roughly corresponds to two disulfide linked Fab fragments having divalent antigen-binding activity and is still capable of cross-linking antigen. Both the Fab and F(ab ’)2 are examples of "antigen- binding fragments." Fab' fragments differ from Fab fragments by having additional few residues at the carboxy terminus of the CHI domain including one or more cysteines from the antibody hinge region. Fab'-SH is the designation herein for Fab' in which the cysteine residue(s) of the constant domains bear a free thiol group. F(ab')2 antibody fragments originally were produced as pairs of Fab' fragments that have hinge cysteines between them. Other chemical couplings of antibody fragments are also known.Fab fragments may be joined, e.g., by a peptide linker, to form a single chain Fab, also referred to herein as "scFab." In these embodiments, an inter-chain disulfide bond that is present in a native Fab may not be present, and the linker serves in full or in part to link or connect the Fab fragments in a single polypeptide chain. A heavy chain- derived Fab fragment (e.g., comprising, consisting of, or consisting essentially of VH + CHI, or "Fd") and a light chain-derived Fab fragment (e.g., comprising, consisting of, or consisting essentially of VL + CL) may be linked in any arrangement to form a WO 2021/226560 PCT/US2021/031442 scFab. For example, a scFab may be arranged, in N-terminal to C-terminal direction, according to (heavy chain Fab fragment - linker - light chain Fab fragment) or (light chain Fab fragment - linker - heavy chain Fab fragment). Peptide linkers and exemplary linker sequences for use in scFabs are discussed in further detail herein."Fv" is a small antibody fragment that contains a complete antigen-recognition and antigen-binding site. This fragment generally consists of a dimer of one heavy- and one light-chain variable region domain in tight, non-covalent association. However, even a single variable domain (or half of an Fv comprising only three CDRs specific for an antigen) has the ability to recognize and bind antigen, although typically at a lower affinity than the entire binding site."Single-chain Fv" also abbreviated as "sFv" or "scFv", are antibody fragments that comprise the Vh and Vl antibody domains connected into a single polypeptide chain. In some embodiments, the scFv polypeptide comprises a polypeptide linker disposed between and linking the Vh and Vl domains that enables the scFv to retain or form the desired structure for antigen binding. Such a peptide linker can be incorporated into a fusion polypeptide using standard techniques well known in the art. For a review of scFv, see Pluckthun in The Pharmacology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315 (1994); Borrebaeck 1995, infra. In certain embodiments, the antibody or antigen-binding fragment comprises a scFv comprising a VH domain, a VL domain, and a peptide linker linking the VH domain to the VL domain. In particular embodiments, a scFv comprises a VH domain linked to a VL domain by a peptide linker, which can be in a VH-linker- VL orientation or in a VL-linker-VH orientation. Any scFv of the present disclosure may be engineered so that the C-terminal end of the VL domain is linked by a short peptide sequence to the N-terminal end of the VH domain, or vice versa (i.e., (N)VL(C)-linker-(N)VH(C) or (N)VH(C)-linker-(N)VL(C). Alternatively, in some embodiments, a linker may be linked to an N-terminal portion or end of the VH domain, the VL domain, or both.Peptide linker sequences may be chosen, for example, based on: (1) their ability to adopt a flexible extended conformation; (2) their inability or lack of ability to adopt a WO 2021/226560 PCT/US2021/031442 secondary structure that could interact with functional epitopes on the first and second polypeptides and/or on a target molecule; and/or (3) the lack or relative lack of hydrophobic or charged residues that might react with the polypeptides and/or target molecule. Other considerations regarding linker design (e.g., length) can include the conformation or range of conformations in which the VH and VL can form a functional antigen-binding site. In certain embodiments, peptide linker sequences contain, for example, Gly, Asn and Ser residues. Other near neutral amino acids, such as Thr and Ala, may also be included in a linker sequence. Other amino acid sequences which may be usefully employed as linker include those disclosed in Maratea et al., Gene 40:39 (1985); Murphy et al., Proc. Natl. Acad. Sci. USA 83:8258 8262 (1986); U.S. Pat. No. 4,935,233, and U.S. Pat. No. 4,751,180. Other illustrative and non-limiting examples of linkers may include, for example, Glu-Gly-Lys-Ser-Ser-Gly-Ser-Gly-Ser-Glu-Ser-Lys- Val-Asp (SEQ ID NO: 19) (Chaudhary et al., Proc. Natl. Acad. Sci. USA 87:1066- 1070 (1990)) and Lys-Glu-Ser-Gly-Ser-Val-Ser-Ser-Glu-Gln-Leu-Ala-Gln-Phe-Arg- Ser-Leu-Asp (SEQ ID NO: 20) (Bird et al., Science 242:423-426 (1988)) and the pentamer Gly-Gly-Gly-Gly-Ser (SEQ ID NO: 21) when present in a single iteration or repeated 1 to 5 or more times, or more; see, e.g., SEQ ID NO: 17. Any suitable linker may be used, and in general can be about 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 15 23, 24, 25, 26, 27, 28, 29, 30, 40, 50, 60, 70, 80, 90, 1amino acids in length, or less than about 200 amino acids in length, and will preferably comprise a flexible structure (can provide flexibility and room for conformational movement between two regions, domains, motifs, fragments, or modules connected by the linker), and will preferably be biologically inert and/or have a low risk of immunogenicity in a human. Exemplary linkers include those comprising or consisting of the amino acid sequence set forth in any one or more of SEQ ID NOs: 10-21. In certain embodiments, the linker comprises or consists of an amino acid sequence having at least 75% (i.e., at least about 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) identity to the amino acid sequence set forth in any one of SEQ ID NOs: 10-21.

WO 2021/226560 PCT/US2021/031442 scFv can be constructed using any combination of the VH and VL sequences or any combination of the CDRH1, CDRH2, CDRH3, CDRLI, CDRL2, and CDRLsequences disclosed herein.In some embodiments, linker sequences are not required; for example, when the first and second polypeptides have non-essential N-terminal amino acid regions that can be used to separate the functional domains and prevent steric interference.In certain embodiments, an antibody or antigen-binding fragment of the present disclosure is monospecific (e.g, binds to a single epitope) or is multispecific (e.g., binds to multiple epitopes and/or target molecules). Antibodies and antigen binding fragments may be constructed in various formats. Exemplary antibody formats disclosed in Spiess et al., Mol. Immunol. 67(2):95 (2015), and in Brinkmann and Kontermann, mAbs 9(2): 182-212 (2017), which formats and methods of making the same are incorporated herein by reference and include, for example, Bispecific T cell Engagers (BiTEs), DARTs, Knobs-Into-Holes (KIH) assemblies, scFv-CH3-KIH assemblies, KIH Common Light-Chain antibodies, TandAbs, Triple Bodies, TriBi Minibodies, Fab-scFv, scFv-CH-CL-scFv, F(ab')2-scFv2, tetravalent HCabs, Intrabodies, CrossMabs, Dual Action Fabs (DAFs) (two-in-one or four-in-one), DutaMabs, DT-IgG, Charge Pairs, Fab-arm Exchange, SEEDbodies, Triomabs, LUZ-Y assemblies, Fcabs, KA-bodies, orthogonal Fabs, DVD-Igs (e.g., US Patent No. 8,258,268, which formats are incorporated herein by reference in their entirety), IgG(H)-scFv, scFv-(H)IgG, IgG(L)-scFv, scFv-(L)IgG, IgG(L,H)-Fv, IgG(H)-V, V(H)- IgG, IgG(L)-V, V(L)-IgG, KIH IgG-scFab, 2scFv-IgG, IgG-2scFv, scFv4-Ig, Zybody, and DVI-IgG (four-in-one), as well as so-called FIT-Ig (e.g., PCT Publication No. WO 2015/103072, which formats are incorporated herein by reference in their entirety), so- called WuxiBody formats (e.g., PCT Publication No. WO 2019/057122, which formats are incorporated herein by reference in their entirety), and so-called In-Elbow-Insert Ig formats (lEI-Ig; e.g., PCT Publication Nos. WO 2019/024979 and WO 2019/025391, which formats are incorporated herein by reference in their entirety).In certain embodiments, the antibody or antigen-binding fragment comprises two or more of VH domains, two or more VL domains, or both (i.e., two or more VH WO 2021/226560 PCT/US2021/031442 domains and two or more VL domains). In particular embodiments, an antigen- binding fragment comprises the format (N-terminal to C-terminal direction) VH-linker-VL-linker-VH-linker-VL, wherein the two VH sequences can be the same or different and the two VL sequences can be the same or different. Such linked scFvs can include any combination of VH and VL domains arranged to bind to a given target, and in formats comprising two or more VH and/or two or more VL, one, two, or more different eptiopes or antigens may be bound. It will be appreciated that formats incorporating multiple antigen-binding domains may include VH and/or VL sequences in any combination or orientation. For example, the antigen-binding fragment can comprise the format VL-linker-VH- linker-VL-linker-VH, VH-linker-VL-linker- VL-linker-VH, or VL-linker-VH- linker-VH-linker-VL.Monospecific or multispecific antibodies or antigen-binding fragments of the present disclosure constructed comprise any combination of the VH and VL sequences and/or any combination of the CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 sequences disclosed herein. A bispecific or multispecific antibody or antigen-binding fragment may, in some embodiments, comprise one, two, or more antigen-binding domains (e.g., a VH and a VL) of the instant disclosure. Two or more binding domains may be present that bind to the same or a different SARS-CoV-2 epitope, and a bispecific or multispecific antibody or antigen-binding fragment as provided herein can, in some embodiments, comprise a further SARS-CoV-2 binding domain, and/or can comprise a binding domain that binds to a different antigen or pathogen altogether.In any of the presently disclosed embodiments, the antibody or antigen- binding fragment can be multispecific; e.g., bispecific, trispecific, or the like.In certain embodiments, the antibody or antigen-binding fragment comprises: (i) a first VH and a first VL; and (ii) a second VH and a second VL, wherein the first VH and the second VH are different and each independently comprise an amino acid sequence having at least 85% (e.g., having 85%, 86%, WO 2021/226560 PCT/US2021/031442 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) dentity to the amino acid sequence set forth in any one of SEQ ID NOs.: 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 298, 312, 322, 332, 350, 351, 353, 359, 361, 363, 365, 367, 368, 369,379, 389, 399, 409, 419, 429, 434, 444, 454, 464, 474, 484, 494, 504, 514, 524, 534,544, 554, 564, 574, 584, 594, 604, 614, 624, 626, 628, 630, 634, 636, 638, 640, 642,644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676,678, 680, 682, 684, 692, 740, 741, 742, 743, 748, 749, 750, 752, 754, 756, 758, 759,761, 762, and 764, and wherein the first VL and the second VL are different and each independently comprise an amino acid sequence having at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133, 143, 154, 157, 168, 194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278, 288, 294, 296, 302, 308, 310, 316, 326, 336,355, 357, 373, 383, 393, 403, 413, 423, 438, 448, 458, 468, 478, 488, 498, 508, 518,528, 538, 548, 558, 568, 578, 588, 598, 608, 618, 686, 696, 738, 744, and 746, andwherein the first VH and the first VL together form a first antigen-binding site, and wherein the second VH and the second VL together form a second antigen-binding site.In certain embodiments, the antibody or antigen-binding fragment comprises (i) a first VH and a first VL; and (ii) a second VH and a second VL, wherein the first VH comprises an amino acid sequence having at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 139 and 342 and the first VL comprises an amino acid sequence having at least 85% (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%)identity to the amino acid sequence set forth in any one of SEQ ID NOs. : 143 and 346 and wherein the second VH comprises an amino acid sequence having at least (e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID WO 2021/226560 PCT/US2021/031442 NOs.: 399, 748, 749, 750, 752, 754, 756, 758, 759, and 761 and the second VL comprises an amino acid sequence having at least 85(e.g., having 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs. :403, 744, and 746.In certain embodiments, the antibody or antigen-binding fragment comprises a Fc polypeptide, or a fragment thereof. The "Fc" fragment or Fc polypeptide comprises the carboxy-terminal portions (i.e., the CH2 and CHdomains of IgG) of both antibody H chains that, in general, are held together by disulfides. Antibody "effector functions " refer to those biological activities attributable to the Fc region (a native sequence Fc region or amino acid sequence variant Fc region) of an antibody, and vary with the antibody isotype. Examples of antibody effector functions include: Clq binding and complement dependent cytotoxicity; Fc receptor binding; antibody-dependent cell-mediated cytotoxicity (ADCC); phagocytosis; down regulation of cell surface receptors (e.g., B cell receptor); and B cell activation. As discussed herein, modifications (e.g., amino acid substitutions) may be made to an Fc domain in order to modify (e.g., improve, reduce, or ablate) one or more functionality of an Fc-containing polypeptide (e.g., an antibody of the present disclosure). Such functions include, for example, Fc receptor (FcR) binding, antibody half-life modulation (e.g., by binding to FcRn), ADCC function, protein A binding, protein G binding, and complement binding. Amino acid modifications that modify (e.g., improve, reduce, or ablate) Fc functionalities include, for example, the T250Q/M428L, M252Y/S254T/T256E, H433K/N434F, M428L/N434S, E233P/L234V/L235A/G236 + A327G/A330S/P331S, E333A, S239D/A330L/I332E, P257I/Q311, K326W/E333S, S239D/I332E/G236A, N297Q, K322A, S228P, L235E + E318A/K320A/K322A, L234A/L235A (also referred to herein as "LALA"), and L234A/L235A/P329G mutations, which mutations are summarized and annotated in "Engineered Fc Regions", published by InvivoGen (2011) and available online at WO 2021/226560 PCT/US2021/031442 1nv1vogen.com/PDF/rev1ew/rev1ew-Engmeered-Fc-Reg1ons- invivogen.pdf?utm_source=review&utm_medium=pdf&utm_ campaign=review&utm_content=Engineered-Fc-Regions, and are incorporated herein by reference.For example, to activate the complement cascade, the Clq protein complex can bind to at least two molecules of IgGl or one molecule of IgM when the immunoglobulin molecule(s) is attached to the antigenic target (Ward, E. S., and Ghetie, V., Ther. Immunol. 2 (1995) 77-94). Burton, D. R., described (MoL Immunol. (1985) 161-206) that the heavy chain region comprising amino acid residues 318 to 337 is involved in complement fixation. Duncan, A. R., and Winter, G. (Nature 3(1988) 738-740), using site directed mutagenesis, reported that Glu318, Lys320 and Lys322 form the binding site to Clq. The role of Glu318, Lys320 and Lys 322 residues in the binding of Clq was confirmed by the ability of a short synthetic peptide containing these residues to inhibit complement mediated lysis.For example, FcR binding can be mediated by the interaction of the Fc moiety (of an antibody) with Fc receptors (FcRs), which are specialized cell surface receptors on cells including hematopoietic cells. Fc receptors belong to the immunoglobulin superfamily, and shown to mediate both the removal of antibody-coated pathogens by phagocytosis of immune complexes, and the lysis of erythrocytes and various other cellular targets (e.g. tumor cells) coated with the corresponding antibody, via antibody dependent cell mediated cytotoxicity (ADCC; Van de Winkel, J. G., and Anderson, C. L., J. Leukoc. Biol. 49 (1991) 511-524). FcRs are defined by their specificity for immunoglobulin classes; Fc receptors for IgG antibodies are referred to as FcyR, for IgE as FceR, for IgA as FcaR and so on and neonatal Fc receptors are referred to as FcRn. Fc receptor binding is described for example in Ravetch, J. V., and Kinet, J. P., Annu. Rev. Immunol. 9 (1991) 457-492; Capel, P. J., et al., Immunomethods 4 (1994) 25-34; de Haas, M., et al., J Lab. Clin. Med. 126 (1995) 330-341; and Gessner, J. E., et al., Ann. Hematol. 76 (1998) 231-248.Cross-linking of receptors by the Fc domain of native IgG antibodies (FcyR) triggers a wide variety of effector functions including phagocytosis, antibody-dependent WO 2021/226560 PCT/US2021/031442 cellular cytotoxicity, and release of inflammatory mediators, as well as immune complex clearance and regulation of antibody production. Fc moi eties providing cross-linking of receptors (e.g., FcyR) are contemplated herein. In humans, three classes of FcyR have been characterized to-date, which are: (i) FcyRI (CD64), which binds monomeric IgG with high affinity and is expressed on macrophages, monocytes, neutrophils and eosinophils; (ii) FcyRII (CD32), which binds complexed IgG with medium to low affinity, is widely expressed, in particular on leukocytes, is believed to be a central player in antibody- mediated immunity, and which can be divided into FcyRIIA, FcyRIIB and FcyRIIC, which perform different functions in the immune system, but bind with similar low affinity to the IgG-Fc, and the ectodomains of these receptors are highly homologuous; and (iii) FcyRIII (CD 16), which binds IgG with medium to low affinity and has been found in two forms: FcyRIIIA, which has been found on NK cells, macrophages, eosinophils, and some monocytes and T cells, and is believed to mediate ADCC; and FcyRIIIB, which is highly expressed on neutrophils.FcyRIIA is found on many cells involved in killing (e.g. macrophages, monocytes, neutrophils) and seems able to activate the killing process. FcyRIIB seems to play a role in inhibitory processes and is found on B-cells, macrophages and on mast cells and eosinophils. Importantly, it has been shown that 75% of all FcyRIIB is found in the liver (Ganesan, L. P. et al., 2012: "FcyRIIb on liver sinusoidal endothelium clears small immune complexes, " Journal of Immunology 189: 4981-4988). FcyRIIB is abundantly expressed on Liver Sinusoidal Endothelium, called LSEC, and in Kupffer cells in the liver and LSEC are the major site of small immune complexes clearance (Ganesan, L. P. et al., 2012: FcyRIIb on liver sinusoidal endothelium clears small immune complexes. Journal of Immunology 189: 4981-4988).In some embodiments, the antibodies disclosed herein and the antigen- binding fragments thereof comprise an Fc polypeptide or fragment thereof for binding to FcyRIIb, in particular an Fc region, such as, for example IgG-type WO 2021/226560 PCT/US2021/031442 antibodies. Moreover, it is possible to engineer the Fc moiety to enhance FcyRIIB binding by introducing the mutations S267E and L328F as described by Chu, S. Y. et al., 2008: Inhibition of B cell receptor-mediated activation of primary human B cells by coengagement of CD19 and FcgammaRIIb with Fc-engineered antibodies. Molecular Immunology 45, 3926-3933. Thereby, the clearance of immune complexes can be enhanced (Chu, S., et al., 2014: Accelerated Clearance of IgE In Chimpanzees Is Mediated By Xmab7195, An Fc-Engineered Antibody With Enhanced Affinity For Inhibitory Receptor FcyRIIb. Am J Respir Crit, American Thoracic Society International Conference Abstracts). In some embodiments, the antibodies of the present disclosure, or the antigen binding fragments thereof, comprise an engineered Fc moiety with the mutations S267E and L328F, in particular as described by Chu, S. Y. et al., 2008: Inhibition of B cell receptor-mediated activation of primary human B cells by coengagement of CD19 and FcgammaRIIb with Fc-engineered antibodies. Molecular Immunology 45, 3926-3933.On B cells, FcyRIIB may function to suppress further immunoglobulin production and isotype switching to, for example, the IgE class. On macrophages, FcyRIIB is thought to inhibit phagocytosis as mediated through FcyRIIA. On eosinophils and mast cells, the B form may help to suppress activation of these cells through IgE binding to its separate receptor.Regarding FcyRI binding, modification in native IgG of at least one of E233- G236, P238, D265, N297, A327 and P329 reduces binding to FcyRI. IgG2 residues at positions 233-236, substituted into corresponding positions IgGl and IgG4, reduces binding of IgGl and IgG4 to FcyRI by 103-fold and eliminated the human monocyte response to antibody-sensitized red blood cells (Armour, K. L., et al. Eur. J. Immunol. 29(1999) 2613-2624).Regarding FcyRII binding, reduced binding for FcyRIIA is found, e.g., for IgG mutation of at least one of E233-G236, P238, D265, N297, A327, P329, D270, Q295, A327, R292 and K414.

WO 2021/226560 PCT/US2021/031442 Two allelic forms of human FcyRIIA are the "H131" variant, which binds to IgGl Fc with high affinity, and the "R131" variant, which binds to IgGl Fc with low affinity. See, e.g., Bruhns et al., Blood 773:3716-37(2009).Regarding FcyRIII binding, reduced binding to FcyRIIIA is found, e.g., for mutation of at least one of E233-G236, P238, D265, N297, A327, P329, D270, Q295, A327, S239, E269, E293, ¥296, V303, A327, K338 and D376. Mapping of the binding sites on human IgGl for Fc receptors, the above- mentioned mutation sites, and methods for measuring binding to FcyRI and FcyRIIA, are described in Shields, R. L., et al., J. Biol. Chem. 276 (2001) 6591- 6604.Two allelic forms of human FcyRIIIA are the "Fl 58" variant, which binds to IgGl Fc with low affinity, and the "VI58" variant, which binds to IgGl Fc with high affinity. See, e.g., Bruhns et at, Blood773:3716-3725 (2009).Regarding binding to FcyRII, two regions of native IgG Fc appear to be involved in interactions between FcyRIIs and IgGs, namely (i) the lower hinge site of IgG Fc, in particular amino acid residues L, L, G, G (234 - 237, EU numbering), and (ii) the adjacent region of the CH2 domain of IgG Fc, in particular a loop and strands in the upper CH2 domain adjacent to the lower hinge region, e.g. in a region of P331 (Wines, B.D., et al., J. Immunol. 2000; 164: 5313-5318). Moreover, FcyRI appears to bind to the same site on IgG Fc, whereas FcRn and Protein A bind to a different site on IgG Fc, which appears to be at the CH2-CH3 interface (Wines, B.D., et al., J. Immunol. 2000; 164: 5313 - 5318).Also contemplated are mutations that increase binding affinity of an Fc polypeptide or fragment thereof of the present disclosure to a (z.e., one or more) Fey receptor (e.g., as compared to a reference Fc polypeptide or fragment thereof or containing the same that does not comprise the mutation(s)). See, e.g., Delillo and Ravetch, Cell 161(5): 1035-1045 (2015) and Ahmed et al., J.

WO 2021/226560 PCT/US2021/031442 Struc. Biol. 194(1):78 (2016), the Fc mutations and techniques of which are incorporated herein by reference.In any of the herein disclosed embodiments, an antibody or antigen-binding fragment can comprise a Fc polypeptide or fragment thereof comprising a mutation selected from G236A; S239D; A330L; and I332E; or a combination comprising any two or more of the same; e.g., S239D/I332E; S239D/A330L/I332E; G236A/S239D/1332E; G236A/A330L/I332E (also referred to herein as "GAALIE"): or G236A/S239D/A330L/1332E. In some embodiments, the Fc polypeptide or fragment thereof does not comprise S239D.In certain embodiments, the Fc polypeptide or fragment thereof may comprise or consist of at least a portion of an Fc polypeptide or fragment thereof that is involved in binding to FcRn binding. In certain embodiments, the Fc polypeptide or fragment thereof comprises one or more amino acid modifications that improve binding affinity for (e.g., enhance binding to) FcRn (e.g., at a pH of about 6.0) and, in some embodiments, thereby extend in vivo half-life of a molecule comprising the Fc polypeptide or fragment thereof (e.g., as compared to a reference Fc polypeptide or fragment thereof or antibody that is otherwise the same but does not comprise the modification(s)). In certain embodiments, the Fc polypeptide or fragment thereof comprises or is derived from a IgG Fc and a half-life-extending mutation comprises any one or more of: M428L; N434S; N434H; N434A; N434S; M252Y; S254T; T256E; T250Q; P257I Q31II; D376V; T307A; E380A (EU numbering). In certain embodiments, a half-life-extending mutation comprises M428L/N434S (also referred to herein as "MLNS"). In certain embodiments, a half-life-extending mutation comprises M252Y/S254T/T256E. In certain embodiments, a half-life-extending mutation comprises T250Q/M428L. In certain embodiments, a half-life-extending mutation comprises P2571/Q3111. In certain embodiments, a half-life-extending mutation comprises P257I/N434H. In certain embodiments, a half-life-extending mutation comprises D376V/N434H. In certain embodiments, a half-life-extending mutation comprises T307A/E380A/N434A.

WO 2021/226560 PCT/US2021/031442 In some embodiments, an antibody or antigen-binding fragment includes a Fc moiety that comprises the substitution mtuations M428L/N434S. In some embodiments, an antibody or antigen-binding fragment includes a Fc polypeptide or fragment thereof that comprises the substitution mtuations G236A/A330L/1332E. In certain embodiments, an antibody or antigen-binding fragment includes a (e.g., IgG) Fc moiety that comprises a G236A mutation, an A330L mutation, and a I332E mutation (GAALIE), and does not comprise a S239D mutation (e.g., comprises a native S at position 239). In particular embodiments, an antibody or antigen-binding fragment includes an Fc polypeptide or fragment thereof that comprises the substitution mutations: M428L/N434S and G236A/A330L/I332E, and optionally does not comprise S239D. In certain embodiments, an antibody or antigen-binding fragment includes a Fc polypeptide or fragment thereof that comprises the substitution mutations: M428L/N434S and G236A/S239D/A330L/I332E.In certain embodiments, the antibody or antigen-binding fragment comprises a mutation that alters glycosylation, wherein the mutation that alters glycosylation comprises N297A, N297Q, or N297G, and/or the antibody or antigen-binding fragment is partially or fully aglycosylated and/or is partially or fully afucosylated. Host cell lines and methods of making partially or fully aglycosylated or partially or fully afucosylated antibodies and antigen-binding fragments are known (see, e.g., PCT Publication No. WO 2016/181357; Suzuki et al. Clin. Cancer Res. 73(6): 1875-82 (2007); Huang et al. MAbs 6:1-(2018)).In certain embodiments, the antibody or antigen-binding fragment is capable of eliciting continued protection in vivo in a subject even once no detectable levels of the antibody or antigen-binding fragment can be found in the subject (i.e., when the antibody or antigen-binding fragment has been cleared from the subject following administration). Such protection is referred to herein as a vaccinal effect. Without wishing to be bound by theory, it is believed that dendritic cells can internalize complexes of antibody and antigen WO 2021/226560 PCT/US2021/031442 and thereafter induce or contribute to an endogenous immune response against antigen. In certain embodiments, an antibody or antigen-binding fragment comprises one or more modifications, such as, for example, mutations in the Fc comprising G236A, A330L, and I332E, that are capable of activating dendritic cells that may induce, e.g., T cell immunity to the antigen.In any of the presently disclosed embodiments, the antibody or antigen-binding fragment comprises a Fc polypeptide or a fragment thereof, including a CH2 (or a fragment thereof, a CH3 (or a fragment thereof), or a CH2 and a CH3, wherein the CH2, the CH3, or both can be of any isotype and may contain amino acid substitutions or other modifications as compared to a corresponding wild-type CH2 or CH3, respectively. In certain embodiments, a Fc polypeptide of the present disclosure comprises two CH2-CH3 polypeptides that associate to form a dimer.In any of the presently disclosed embodiments, the antibody or antigen-binding fragment can be monoclonal. The term "monoclonal antibody" (mAb) as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present, in some cases in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to polyclonal antibody preparations that include different antibodies directed against different epitopes, each monoclonal antibody is directed against a single epitope of the antigen. In addition to their specificity, the monoclonal antibodies are advantageous in that they may be synthesized uncontaminated by other antibodies. The term "monoclonal" is not to be construed as requiring production of the antibody by any particular method. For example, monoclonal antibodies useful in the present invention may be prepared by the hybridoma methodology first described by Kohler etal., Nature 256A95 (1975), or may be made using recombinant DNA methods in bacterial, eukaryotic animal, or plant cells (see, e.g., U.S. Pat. No. 4,816,567). Monoclonal antibodies may also be isolated from phage antibody libraries using the techniques described in Clackson et al., Nature, 352:624-628 (1991) and Marks et al., J. Mol. Biol., 222:581-597 (1991), for example.

WO 2021/226560 PCT/US2021/031442 Monoclonal antibodies may also be obtained using methods disclosed in PCT Publication No. WO 2004/076677A2.Antibodies and antigen-binding fragments of the present disclosure include "chimeric antibodies" in which a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (see, U.S. Pat. Nos. 4,816,567; 5,530,101 and 7,498,415; and Morrison et al.. Proc. Natl. Acad. Sci. USA, 87:6851-6855 (1984)). For example, chimeric antibodies may comprise human and non-human residues. Furthermore, chimeric antibodies may comprise residues that are not found in the recipient antibody or in the donor antibody. These modifications are made to further refine antibody performance. For further details, see Jones et al., Nature 321:522-525 (1986); Riechmann et at, Nature 332:323-329 (1988); and Presta, Curr. Op. Struct. Biol. 2:593-596 (1992). Chimeric antibodies also include primatized and humanized antibodies.A "humanized antibody" is generally considered to be a human antibody that has one or more amino acid residues introduced into it from a source that is non-human. These non-human amino acid residues are typically taken from a variable domain. Humanization may be performed following the method of Winter and co-workers (Jones etal., Nature, 321:522-525 (1986); Reichmann et at, Nature, 332:323-327 (1988); Verhoeyen et al., Science, 239:1534-15(1988)), by substituting non-human variable sequences for the corresponding sequences of a human antibody. Accordingly, such "humanized" antibodies are chimeric antibodies (U.S. Pat. Nos. 4,816,567; 5,530,101 and 7,498,415) wherein substantially less than an intact human variable domain has been substituted by the corresponding sequence from a non-human species. In some WO 2021/226560 PCT/US2021/031442 instances, a "humanized " antibody is one which is produced by a non-human cell or animal and comprises human sequences, e.g., He domains.A "human antibody" is an antibody containing only sequences that are present in an antibody that is produced by a human. However, as used herein, human antibodies may comprise residues or modifications not found in a naturally occurring human antibody (e.g., an antibody that is isolated from a human), including those modifications and variant sequences described herein. These are typically made to further refine or enhance antibody performance. In some instances, human antibodies are produced by transgenic animals. For example, see U.S. Pat. Nos. 5,770,429; 6,596,541 and 7,049,426.In certain embodiments, an antibody or antigen-binding fragment of the present disclosure is chimeric, humanized, or human.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of binding to a SARS-CoV-2 surface glycoprotein with an ECof less than 500 ng/ml, less than 250 ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 16 ng/ml, less than 15 ng/ml, less than 14 ng/ml, less than 13 ng/ml, less than 12 ng/ml, less than ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, or less than 2 mg/ml, as measured by ELISA (optionally, indirect ELISA and/or sandwich ELISA) and/or by flow cytometry, wherein the SARS C0V-2 surface glycoprotein is expressed at a cell surface of a host cell.In some embodiments, antibody or antigen-binding fragment of the present disclosure is capable of binding to a SARS-CoV-2 surface glycoprotein RED with an EC50 of less than 500 ng/ml, less than 250 ng/ml, less than 100 ng/ml, less than ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than ng/ml, less than 15 ng/ml, less than 14 ng/ml, less than 13 ng/ml, less than 12 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than WO 2021/226560 PCT/US2021/031442 ng/ml, less than 5 ng/ml, less than 4 ng/ml, or less than 2 mg/ml, as measured by ELISA (optionally, indirect ELISA and/or sandwich ELISA) and/or by flow cytometry, wherein the SARS C0V-2 surface glycoprotein is expressed at a cell surface of a host cell.In some embodiments, antibody or antigen-binding fragment of the present disclosure is capable of binding to a SARS-CoV-2 RED with a KD of less than 5 x 108־ M, less than 4 x 108־ M, less than 3 x 108־ M, less than 2 x 108־ M, less than 1 x 108־ M, less than 5 x 109־ M, less than 1 x 109־ M, less than 5 x 1010־ M, less than 1 x 1010־ M, less than 5 x 1011־ M, less than 1 x 1011־ M, less than 5 x 1012־ M, or less than 1 x 1012־ M, as determined using biolayer interferometry (BLI), optionally using an Octet instrument with antibody or antigen-binding fragment loaded on Protein A pins, optionally at 2.7 ug/ml, and SARS-CoV-2 RBD loaded for 5 minutes at 6 ug/ml, 1.ug/ml, or 0.4 ug/ml, further optionally with dissociation measured for 7 minutes.In some embodiments, antibody or antigen-binding fragment of the present disclosure is capable of binding to a SARS-CoV-2 RBD with a KD of less than 6 x 108־ M, less than 5 x 108־ M, less than 4 x 108־ M, less than 3 x 108־ M, less than 2 x 108־ M, less than 1 x 108־ M, less than 5 x 109־ M, less than 4 x 109־ M, less than 3 x 109־ M, less than 2 x 109־ M, less than 1 x 109־ M, or less than 8 x 1010־ M, as determined using surface plasmon resonance (SPR), optionally using a Biacore T200 instrument using a single-cycle kinetics approach.In some embodiments, antibody or antigen-binding fragment of the present disclosure is capable of binding to a SARS-CoV-2 RBD and inhibiting an interaction between (i) the RBD and (ii) a human ACE2 and/or a human SIGLEC-1.In some embodiments, antibody or antigen-binding fragment of the present disclosure is capable of neutralizing: (i) infection by a SARS-CoV-2 pseudovirus, optionally: (i)(a) with a neutralization IC50 of less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than ng/ml, less than 5 ng/ml, less than 4 ng/ml, less than 3 ng/ml, less than 2 ng/ml, or less WO 2021/226560 PCT/US2021/031442 than 1 ng/ml, preferably less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, less than 3 ng/ml, less than 2 ng/ml, or less than 1 ng/ml, and/or (i)(b) with a neutralization IC80 of less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than ng/ml, less than 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, or less than 25 ng/ml, preferably less than 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, or less than ng/ml, and/or (i)(c) with a neutralization EC90 of less than 300 ng/ml, less than 2ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, or less than 10 ng/ml, wherein, further optionally, the SARS-CoV-2 pseudovirus comprises a VSV pseudovirus and/or a MLV pseudovirus, and/or (i)(d) the SARS-CoV-2 pseudovirus comprises a VSV pseudovirus and/or a MLV pseudovirus; and/or (ii) infection by live SARS-CoV-2, optionally (ii)(a) with a EC50 of less than 60 ng/ml, less than 50 ng/ml, less than 40 ng/ml, less than ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, preferably less than ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, and/or (ii)(b) with a EC90 of less than 50 ng/ml, less than 40 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, preferably less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, or less than 12 ng/ml, and/or (ii)(c) over a 6-hour period, with a multiplicity of infection of 0.1; and/or (iii) infection by live SARS-CoV-2 in a host cell (e.g. a HEK293T cell) that expresses, optionally is engineered to overexpress, DC-SIGN, L-SIGN, SIGLEC, or ACE2; and/or (iv) infection by live SARS-CoV-2 in a host cell (e.g. a HEK293T cell) that expresses, optionally is engineered to overexpress, SIGLEC-1 or ACE2, wherein neutralizing infection comprises fully neutralizing infection.

WO 2021/226560 PCT/US2021/031442 In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing infection by a SARS-C0V-2 variant that comprises any one of the following mutations in the surface glycoprotein as compared to a SARS- C0V-2 surface glycoprotein comprising SEQ ID NO.:3: N501Y; S477N; N439K; L452R; E484K; K417N; T478K; S494P; A520S; N501T; A522S; Y453F; P384L.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing infection by the SARS-C0V-2 variant with a potency that is less than 3-fold lower than the potency with which the antibody or antigen-binding fragment neutralizes infection by a SARS-C0V-2 comprising the surface glycoprotein amino acid sequence set forth in SEQ ID NO. :3.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of activating a FcyRIIa, a FcyRIIIa, or both, wherein, optionally:(!) the FcyRIIa comprises a H131 allele; and/or (ii) the FcyRIIIa comprises a V158 allele; and/or (iii) activation is determined using a SARS-C0V-2 S-expressing target cell, such as a CHO cell, and a reporter cell expressing a NFAT-driven reporter, such as luciferase.In some embodiments, an antibody or antigen-binding fragment of the present disclosure has an in vivo half-life in a non-human primate of between 20 and 30 days, or between 22 and 28 days, or between 23 and 27 days, or between 24 and 26 days, or of about 25 days.In some embodiments an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing a SARS-C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 20 to about 30 ng/ml.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing a SARS-C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 10 to about 20 ng/ml.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing a SARS-C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 5 to about 10 ng/ml.

WO 2021/226560 PCT/US2021/031442 In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing a SARS-C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 1 to about 5 ng/ml.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is capable of neutralizing infection by SARS-C0V-2 and does not compete with a human ACE2 for binding to the SARS-CoV-2S protein, wherein, optionally, the neutralizing comprises neutralizing infection in an in vitro model of infection.In some embodiments, an antibody or antigen-binding fragment of the present disclosure is provided that competes for binding to a SARS-CoV-2 surface glycoprotein with an antibody or antigen-binding fragment of the present disclosure.

Polynucleotides, Vectors, and Host cells In another aspect, the present disclosure provides isolated polynucleotides that encode any of the presently disclosed antibodies or an antigen-binding fragment thereof, or a portion thereof (e.g., a CDR, a VH, a VL, a heavy chain, or a light chain). In certain embodiments, the polynucleotide is codon-optimized for expression in a host cell. Once a coding sequence is known or identified, codon optimization can be performed using known techniques and tools, e.g., using the GenScript® OptimiumGene™ tool; see also Scholten etal., Clin. Immunol. 779:135, 2006). Codon-optimized sequences include sequences that are partially codon-optimized (i.e., one or more codon is optimized for expression in the host cell) and those that are fully codon-optimized.It will also be appreciated that polynucleotides encoding antibodies and antigen- binding fragments of the present disclosure may possess different nucleotide sequences while still encoding a same antibody or antigen-binding fragment due to, for example, the degeneracy of the genetic code, splicing, and the like.In certain embodiments, the polynucleotide comprises a polynucleotide having at least 50% (i.e., 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the polynucleotide sequence according to any one or more of SEQ ID NOs.:30, 31, 40, 41, 50, 51, 60, 61, 70, 71, 73, WO 2021/226560 PCT/US2021/031442 82, 83, 92, 93, 95, 104, 105, 114, 115, 116, 117, 118, 127, 128, 137, 138, 206, 207, 216, 217, 226, 227, 236, 237, 239, 248, 249, 251, 253, 262, 263, 272, 273,282, 283, 292, 293, 295, 297, 306, 307, 309, 311, 320, 321, 330, 331, 340, 341,377, 378, 387, 388, 397, 398, 407, 408, 417, 418, 427, 428, 433, 442, 443, 452,453, 462, 463, 472, 473, 482, 483, 492, 493, 502, 503, 512, 513, 552, 523, 532,533, 542, 543, 552, 553, 562, 563, 572, 573, 582, 583, 592, 593, 602, 603, 612,613, 622, 623, 690, 691, 700-737, and 739.In certain embodiments, a polynucleotide comprises (i) a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or that comprises or consists of, the nucleotide sequence set forth in SEQ ID NO. :407; and (ii) a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or that comprises or consists of, the nucleotide sequence set forth in SEQ ID NO. :408, 737, or 739.In any of the presently disclosed embodiments, the polynucleotide can comprise deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). In some embodiments, the RNA comprises messenger RNA (mRNA).Vectors are also provided, wherein the vectors comprise or contain a polynucleotide as disclosed herein (e.g., a polynucleotide that encodes an antibody or antigen-binding fragment that binds to SARS-C0V-2). A vector can comprise any one or more of the vectors disclosed herein. In particular embodiments, a vector is provided that comprises a DNA plasmid construct encoding the antibody or antigen-binding fragment, or a portion thereof (e.g., so-called "DMAb"; see, e.g., Muthumani etal., J Infect Dis. 214(3f.369-3I% (2016); Muthumani et al., Hum Vaccin Immunother 9:2253-2262 (2013)); Flingai et al, Set Rep. 5:12616 (2015); and Elliott et al, NPJ Vaccines (2017), which antibody-coding DNA constructs and related methods of use, including administration of the same, are incorporated herein by reference). In certain embodiments, a DNA plasmid construct comprises a single open reading WO 2021/226560 PCT/US2021/031442 frame encoding a heavy chain and a light chain (or a VH and a VL) of the antibody or antigen-binding fragment, wherein the sequence encoding the heavy chain and the sequence encoding the light chain are optionally separated by polynucleotide encoding a protease cleavage site and/or by a polynucleotide encoding a self-cleaving peptide. In some embodiments, the substituent components of the antibody or antigen-binding fragment are encoded by a polynucleotide comprised in a single plasmid. In other embodiments, the substituent components of the antibody or antigen-binding fragment are encoded by a polynucleotide comprised in two or more plasmids (e.g., a first plasmid comprises a polynucleotide encoding a heavy chain, VH, or VH+CH, and a second plasmid comprises a polynucleotide encoding the cognate light chain, VL, or VL+CL). In certain embodiments, a single plasmid comprises a polynucleotide encoding a heavy chain and/or a light chain from two or more antibodies or antigen- binding fragments of the present disclosure. An exemplary expression vector is pVaxl, available from Invitrogen®. A DNA plasmid of the present disclosure can be delivered to a subject by, for example, electroporation (e.g., intramuscular electroporation), or with an appropriate formulation (e.g., hyaluronidase).In a further aspect, the present disclosure also provides a host cell expressing an antibody or antigen-binding fragment according to the present disclosure; or comprising or containing a vector or polynucleotide according the present disclosure.Examples of such cells include but are not limited to, eukaryotic cells, e.g., yeast cells, animal cells, insect cells, plant cells; and prokaryotic cells, including E. coll. In some embodiments, the cells are mammalian cells. In certain such embodiments, the cells are a mammalian cell line such as CHO cells (e.g., DHFR- CHO cells (Urlaub et at, PNAS 77:4216 (1980)), human embryonic kidney cells (e.g., HEK293T cells), PER.C6 cells, ¥0 cells, Sp2/0 cells. NS0 cells, human liver cells, e.g. Hepa RG cells, myeloma cells or hybridoma cells. Other examples of mammalian host cell lines include mouse sertoli cells (e.g., TM4 cells); monkey kidney CV1 line transformed by SV40 (COS-7); baby hamster kidney cells (BHK); African green monkey kidney cells (VERO-76); monkey kidney cells (CV1); human cervical carcinoma cells (HELA); human lung cells (W138); human liver cells (Hep G2); canine kidney cells (MDCK; WO 2021/226560 PCT/US2021/031442 buffalo rat liver cells (BRL 3 A); mouse mammary tumor (MMT 060562); TRI cells; MRC 5 cells; and FS4 cells. Mammalian host cell lines suitable for antibody production also include those described in, for example, Yazaki and W'u, Methods in Molecular Biology, Vol. 248 (B. K. C. Lo, ed., Humana Press, Totowa, N.J.), pp. 255-268 (2003).In certain embodiments, a host cell is a prokaryotic cell, such as an E. coli. The expression of peptides in prokaryotic cells such as E. coli is well established (see, e.g., Pluckthun, A. Bio/Technology 9:545-551 (1991). For example, antibodies may be produced in bacteria, in particular when glycosylation and Fc effector function are not needed. For expression of antibody fragments and polypeptides in bacteria, see, e.g., U.S. Pat. Nos. 5,648,237; 5,789,199; and 5,840,523.In particular embodiments, the cell may be transfected with a vector according to the present description with an expression vector. The term "transfection" refers to the introduction of nucleic acid molecules, such as DNA or RNA (e.g. mRNA) molecules, into cells, such as into eukaryotic cells. In the context of the present description, the term "transfection" encompasses any method known to the skilled person for introducing nucleic acid molecules into cells, such as into eukaryotic cells, including into mammalian cells. Such methods encompass, for example, electroporation, lipofection, e.g., based on cationic lipids and/or liposomes, calcium phosphate precipitation, nanoparticle based transfection, virus based transfection, or transfection based on cationic polymers, such as DEAE-dextran or polyethylenimine, etc. In certain embodiments, the introduction is non-viral.Moreover, host cells of the present disclosure may be transfected stably or transiently with a vector according to the present disclosure, e.g. for expressing an antibody, or an antigen-binding fragment thereof, according to the present disclosure. In such embodiments, the cells may be stably transfected with the vector as described herein. Alternatively, cells may be transiently transfected with a vector according to the present disclosure encoding an WO 2021/226560 PCT/US2021/031442 antibody or antigen-binding fragment as disclosed herein. In any of the presently disclosed embodiments, a polynucleotide may be heterologous to the host cell.Accordingly, the present disclosure also provides recombinant host cells that heterologously express an antibody or antigen-binding fragment of the present disclosure. For example, the cell may be of a species that is different to the species from which the antibody was fully or partially obtained (e.g., CHO cells expressing a human antibody or an engineered human antibody). In some embodiments, the cell type of the host cell does not express the antibody or antigen-binding fragment in nature. Moreover, the host cell may impart a post-translational modification (PTM; e.g., glysocylation or fucosylation) on the antibody or antigen-binding fragment that is not present in a native state of the antibody or antigen-binding fragment (or in a native state of a parent antibody from which the antibody or antigen binding fragment was engineered or derived). Such a PTM may result in a functional difference (e.g., reduced immunogenicity). Accordingly, an antibody or antigen-binding fragment of the present disclosure that is produced by a host cell as disclosed herein may include one or more post-translational modification that is distinct from the antibody (or parent antibody) in its native state (e.g., a human antibody produced by a CHO cell can comprise a more post-translational modification that is distinct from the antibody when isolated from the human and/or produced by the native human B cell or plasma cell).Insect cells useful expressing a binding protein of the present disclosure are known in the art and include, for example, Spodoptera frugipera Sf9 cells, Trichoplusia ni BTI-TN5B1-4 cells, and Spodopterafrugipera SfSWTOl "Mimic™ " cells. See, e.g., Palmberger et al., J. Biotechnol. 753(3-4): 160-166 (2011). Numerous baculoviral strains have been identified which may be used in conjunction with insect cells, particularly for transfection of Spodoptera frugiperda cells.Eukaryotic microbes such as filamentous fungi or yeast are also suitable hosts for cloning or expressing protein-encoding vectors, and include fungi and yeast strains with "humanized" glycosylation pathways, resulting in the production of an antibody with a partially or fully human glycosylation pattern. See Gerngross, Nat. Biotech. 22:1409-1414 (2004); Li et aL Nat. Biotech. 24:210-215 (2006).

WO 2021/226560 PCT/US2021/031442 Plant cells can also be utilized as hosts for expressing a binding protein of the present disclosure. For example, PLANTIBODIES™ technology (described in, for example, U.S. Pat. Nos. 5,959,177; 6,040,498; 6,420,548; 7,125,978; and 6,417,429) employs transgenic plants to produce antibodies.In certain embodiments, the host cell comprises a mammalian cell. In particular embodiments, the host cell is a CHO cell, a HEK293 cell, a PER.Ccell, a ¥0 cell, a Sp2/0 cell, aNSO cell, a human liver cell, a myeloma cell, or a hybridoma cell.In a related aspect, the present disclosure provides methods for producing an antibody, or antigen-binding fragment, wherein the methods comprise culturing a host cell of the present disclosure under conditions and for a time sufficient to produce the antibody, or the antigen-binding fragment. Methods useful for isolating and purifying recombinantly produced antibodies, by way of example, may include obtaining supernatants from suitable host cell/vector systems that secrete the recombinant antibody into culture media and then concentrating the media using a commercially available filter. Following concentration, the concentrate may be applied to a single suitable purification matrix or to a series of suitable matrices, such as an affinity matrix or an ion exchange resin. One or more reverse phase HPLC steps may be employed to further purify a recombinant polypeptide. These purification methods may also be employed when isolating an immunogen from its natural environment. Methods for large scale production of one or more of the isolated/recombinant antibody described herein include batch cell culture, which is monitored and controlled to maintain appropriate culture conditions. Purification of soluble antibodies may be performed according to methods described herein and known in the art and that comport with laws and guidelines of domestic and foreign regulatory agencies.

Compositions Also provided herein are compositions that comprise any one or more of the presently disclosed antibodies, antigen-binding fragments, polynucleotides, vectors, or WO 2021/226560 PCT/US2021/031442 host cells, singly or in any combination, and can further comprise a pharmaceutically acceptable carrier, excipient, or diluent. Carriers, excipients, and diluents are discussed in further detail herein.In certain embodiments, a composition comprises two or more different antibodies or antigen-binding fragments according to the present disclosure. In certain embodiments, antibodies or antigen-binding fragments to be used in a combination each independently have one or more of the following characteristics: neutralize naturally occurring SARS-CoV-2 variants; do not compete with one another for Spike protein binding; bind distinct Spike protein epitopes; have a reduced formation of resistance to SARS-CoV-2; when in a combination, have a reduced formation of resistance to SARS-CoV-2; potently neutralize live SARS-CoV-2 virus; exhibit additive or synergistic effects on neutralization of live SARS-CoV-2 virus when used in combination; exhibit effector functions; are protective in relevant animal model(s) of infection; are capable of being produced in sufficient quantities for large-scale production.In certain embodiments, a composition comprises two or more different antibodies or antigen-binding fragments, which can be two or more presently disclosed antibodies or antigen-binding fragments. In any of the presently disclosed embodiments, an antibody or antigen-binding fragment thereof can be comprised in a composition that further comprises an antibody or antigen-binding fragment that comprises: (i) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs. :343-345 and 347-349, respectively; or (ii) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.: 140-142 and 144-146, respectively; or (iii) VH and VL amino acid sequences as set forth in SEQ ID NOs. :342 and 346, respectively; or (iv) VH and VL amino acid sequences as set forth in SEQ ID NOs. :139 and 143, respectively.In certain embodiments, a composition comprises a first antibody or antigen- binding fragment, comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 32 and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 36; and a second antibody or antigen ­ WO 2021/226560 PCT/US2021/031442 binding fragment comprising a VH comprising or consisting of the ammo acid sequence as set forth in SEQ ID NO: 139, and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 143. In certain embodiments, a composition comprises a first antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 33-35, respectively, and the CDRL1, CDRL2, and CDRLcomprise or consist of the amino acid sequences set forth in SEQ ID NOs: 37-39, respectively, and a second antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 140-142, respectively, and the CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 144-146, respectively.In certain embodiments, a composition comprises a first antibody or antigen- binding fragment, comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 139 or 342 and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 143 or 346; and a second antibody or antigen-binding fragment comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761, and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 403, 744, or 746. In certain embodiments, a composition comprises a first antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 140-142, respectively, or 343-345, respectively, and the CDRL1, CDRL2, and CDRLcomprise or consist of the amino acid sequences set forth in SEQ ID NOs: 144-146, WO 2021/226560 PCT/US2021/031442 respectively, and a second antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ IDNOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively, and the CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively.Also provided herein are compositions that comprise (i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS-CoV-2 surface glycoprotein and a first cell surface receptor selected from ACE2, DC-SIGN, L-SIGN, and SIGLEC-1; and (ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS-CoV-2 surface glycoprotein and a second cell surface receptor selected from ACE2, DC-SIGN, L-SIGN, and SIGLEC-1, wherein the first cell surface receptor and the second cell surface receptor are different. As taught in the present disclosure, neutralization of infection can be achieved or improved by combingin antibodies or antigen-binding fragments, the binding of which to SARS-CoV-2 inhibits interactions between the SARS-CoV-2 and two or more cell surface receptors; e.g., an attachment receptor and an entry receptor, two entry receptors, two attachment receptors, or the like. Methods of using such antibody combinations to treat or prevent a SARS-CoV-infection are also provided.In certain embodiments, a composition comprises a first vector comprising a first plasmid, and a second vector comprising a second plasmid, wherein the first plasmid comprises a polynucleotide encoding a heavy chain, VH, or VH+CH, and a second plasmid comprises a polynucleotide encoding the cognate light chain, VL, or VL+CL of the antibody or antigen-binding fragment thereof. In certain embodiments, a composition comprises a polynucleotide (e.g., mRNA) coupled to a suitable delivery vehicle or carrier. Exemplary vehicles or carriers for administration to a human subject WO 2021/226560 PCT/US2021/031442 include a lipid or hpid-denved delivery vehicle, such as a liposome, solid lipid nanoparticle, oily suspension, submicron lipid emulsion, lipid microbubble, inverse lipid micelle, cochlear liposome, lipid microtubule, lipid microcylinder, or lipid nanoparticle (LNP) or a nanoscale platform (see, e.g., Li et al. Wilery Interdiscip Rev. Nanomed Nanobiotechnol. 77(2):el530 (2019)). Principles, reagents, and techniques for designing appropriate mRNA and and formulating mRNA-LNP and delivering the same are described in, for example, Pardi et al. (J Control Release 277345-351 (2015)); Thess et al. (Mol Ther 23: 1456-1464 (2015)); Thran et al. (EMBO Mol Med 9(10): 1434-1448 (2017); Kose et al. (Set. Immunol. 4 eaaw6647 (2019); and Sabnis et al. (Mol. Ther. 26:1509-1519 (2018)), which techniques, include capping, codon optimization, nucleoside modification, purification of mRNA, incorporation of the mRNA into stable lipid nanoparticles (e.g., ionizable cationic lipid/phosphatidylcholine/cholesterol/PEG-lipid; ionizable lipid:distearoyl PC:cholesterol:polyethylene glycol lipid), and subcutaneous, intramuscular, intradermal, intravenous, intraperitoneal, and intratracheal administration of the same, are incorporated herein by reference.

Methods and Uses Also provided herein are methods for use of an antibody or antigen- binding fragment, nucleic acid, vector, cell, or composition of the present disclosure in the diagnosis of SARS-C0V-2 infection (e.g, in a human subject, or in a sample obtained from a human subject).Methods of diagnosis (e.g., in vitro, ex vivo) may include contacting an antibody, antibody fragment (e.g., antigen binding fragment) with a sample. Such samples may be isolated from a subject, for example an isolated tissue sample taken from, for example, nasal passages, sinus cavities, salivary glands, lung, liver, pancreas, kidney, ear, eye, placenta, alimentary tract, heart, ovaries, pituitary, adrenals, thyroid, brain, skin or blood. The methods of diagnosis may also include the detection of an antigen/antibody complex, in particular following the contacting of an antibody or antibody fragment with a sample. Such a detection step can be performed at the bench, i.e. without any contact to WO 2021/226560 PCT/US2021/031442 the human or animal body. Examples of detection methods are well-known to the person skilled in the art and include, e.g., ELISA (enzyme-linked immunosorbent assay), including direct, indirect, and sandwich ELISA. Other detection methods include, but are not limited to, immunohistochemistry (IHC), flow cytometry (e.g., FACS), Western blot, immunocytochemistry (ICC), enzyme-linked immunospot (ELISPOT), and immunoprecipitation (IP). Antibodies and antigen-binding fragments used in detection methods can be, for example, fluorescently or otherwise detectably labeled (e.g., directly conjugated to a fluorophore or comprising a fluorophore- secondary conjugate).Also provided herein are methods of treating a subject using an antibody or antigen-binding fragment of the present disclosure, or a composition comprising the same, wherein the subject has, is believed to have, or is at risk for having an infection by SARS-CoV-2. "Treat," "treatment," or "ameliorate" refers to medical management of a disease, disorder, or condition of a subject (e.g., a human or non-human mammal, such as a primate, horse, cat, dog, goat, mouse, or rat). In general, an appropriate dose or treatment regimen comprising an antibody or composition of the present disclosure is administered in an amount sufficient to elicit a therapeutic or prophylactic benefit. Therapeutic or prophylactic/preventive benefit includes improved clinical outcome; lessening or alleviation of symptoms associated with a disease; decreased occurrence of symptoms; improved quality of life; longer disease-free status; diminishment of extent of disease, stabilization of disease state; delay or prevention of disease progression; remission; survival; prolonged survival; or any combination thereof. In certain embodiments, therapeutic or prophylactic/preventive benefit includes reduction or prevention of hospitalization for treatment of a SARS-CoV-2 infection (i.e., in a statistically significant manner). In certain embodiments, therapeutic or prophylactic/preventive benefit includes a reduced duration of hospitalization for treatment of a SARS-CoV-2 infection (i.e., in a statistically significant manner). In certain embodiments, therapeutic or prophylactic/preventive benefit includes a reduced or abrogated need for respiratory intervention, such as intubation and/or the use of a WO 2021/226560 PCT/US2021/031442 respirator device. In certain embodiments, therapeutic or prophylactic/preventive benefit includes reversing a late-stage disease pathology and/or reducing mortality.A "therapeutically effective amount" or "effective amount" of an antibody, antigen-binding fragment, polynucleotide, vector, host cell, or composition of this disclosure refers to an amount of the composition or molecule sufficient to result in a therapeutic effect, including improved clinical outcome; lessening or alleviation of symptoms associated with a disease; decreased occurrence of symptoms; improved quality of life; longer disease-free status; diminishment of extent of disease, stabilization of disease state; delay of disease progression; remission; survival; or prolonged survival in a statistically significant manner. When referring to an individual active ingredient, administered alone, a therapeutically effective amount refers to the effects of that ingredient or cell expressing that ingredient alone. When referring to a combination, a therapeutically effective amount refers to the combined amounts of active ingredients or combined adjunctive active ingredient with a cell expressing an active ingredient that results in a therapeutic effect, whether administered serially, sequentially, or simultaneously. A combination may comprise, for example, two different antibodies that specifically bind a SARS- C0V-2 antigen, which in certain embodiments, may be the same or different SARS-CoV-2 antigen, and/or can comprise the same or different epitopes.Accordingly, in certain embodiments, methods are provided for treating a SARS-CoV-2 infection in a subject, wherein the methods comprise administering to the subject an effective amount of an antibody, antigen-binding fragment, polynucleotide, vector, host cell, or composition as disclosed herein.Subjects that can be treated by the present disclosure are, in general, human and other primate subjects, such as monkeys and apes for veterinary medicine purposes. Other model organisms, such as mice and rats, may also be treated according to the present disclosure. In any of the aforementioned embodiments, the subject may be a human subject. The subjects can be male or WO 2021/226560 PCT/US2021/031442 female and can be any suitable age, including infant, juvenile, adolescent, adult, and geriatric subjects.A number of criteria are believed to contribute to high risk for severe symptoms or death associated with a SARS C0V-2 infection. These include, but are not limited to, age, occupation, general health, pre-existing health conditions, and lifestyle habits. In some embodiments, a subject treated according to the present disclosure comprises one or more risk factors.In certain embodiments, a human subject treated according to the present disclosure is an infant, a child, a young adult, an adult of middle age, or an elderly person. In certain embodiments, a human subject treated according to the present disclosure is less than 1 year old, or is 1 to 5 years old, or is between 5 and 125 years old (e.g., 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, or 125 years old, including any and all ages therein or therebetween). In certain embodiments, a human subject treated according to the present disclosure is 0- years old, 20-44 years old, 45-54 years old, 55-64 years old, 65-74 years old, 75-years old, or 85 years old, or older. Persons of middle, and especially of elderly age are believed to be at particular risk. In particular embodiments, the human subject is 45-years old, 55-64 years old, 65-74 years old, 75-84 years old, or 85 years old, or older. In some embodiments, the human subject is biologically male. In some embodiments, the human subject is biologically female.In certain embodiments, a human subject treated according to the present disclosure is a resident of a nursing home or a long-term care facility, is a hospice care worker, is a healthcare provider or healthcare worker, is a first responder, is a family member or other close contact of a subject diagnosed with or suspected of having a SARS-CoV-2 infection, is overweight or clinically obese, is or has been a smoker, has or had chronic obstructive pulmonary disease (COPD), is asthmatic (e.g., having moderate to severe asthma), has an autoimmune disease or condition (e.g., diabetes), and/or has a compromised or depleted immune system (e.g., due to AIDS/HIV infection, a cancer such as a blood cancer, a lymphodepleting therapy such as a chemotherapy, a bone marrow or organ transplantation, or a genetic immune condition), WO 2021/226560 PCT/US2021/031442 has chronic liver disease, has cardiovascular disease, has a pulmonary or heart defect, works or otherwise spends time in close proximity with others, such as in a factory, shipping center, hospital setting, or the like.In certain embodiments, a subject treated according to the present disclosure has received a vaccine for SARS-C0V-2 and the vaccine is determined to be ineffective (i.e., at least partially, or completely, ineffective), e.g., by post-vaccine infection or symptoms in the subject, by clinical diagnosis or scientific or regulatory consensus.In certain embodiments, treatment is administered as peri-exposure prophylaxis. In certain embodiments, treatment is administered to a subject with mild-to-moderate disease, which may be in an outpatient setting. In certain embodiments, treatment is administered to a subject with moderate-to-severe disease, such as requiring hospitalization.Typical routes of administering the presently disclosed compositions thus include, without limitation, oral, topical, transdermal, inhalation, parenteral, sublingual, buccal, rectal, vaginal, and intranasal. The term "parenteral", as used herein, includes subcutaneous injections, intravenous, intramuscular, intrasternal injection or infusion techniques. In certain embodiments, administering comprises administering by a route that is selected from oral, intravenous, parenteral, intragastric, intrapleural, intrapulmonary, intrarectal, intradermal, intraperitoneal, intratumoral, subcutaneous, topical, transdermal, intracisternal, intrathecal, intranasal, and intramuscular. In particular embodiments, a method comprises orally administering the antibody, antigen- binding fragment, polynucleotide, vector, host cell, or composition to the subject.Pharmaceutical compositions according to certain embodiments of the present invention are formulated so as to allow the active ingredients contained therein to be bioavailable upon administration of the composition to a patient. Compositions that will be administered to a subject or patient may take the form of one or more dosage units, where for example, a tablet may be a single dosage WO 2021/226560 PCT/US2021/031442 unit, and a container of a herein described an antibody or antigen-binding in aerosol form may hold a plurality of dosage units. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington: The Science and Practice of Pharmacy, 20th Edition (Philadelphia College of Pharmacy and Science, 2000). The composition to be administered will, in any event, contain an effective amount of an antibody or antigen-binding fragment, polynucleotide, vector, host cell, or composition of the present disclosure, for treatment of a disease or condition of interest in accordance with teachings herein.A composition may be in the form of a solid or liquid. In some embodiments, the carrier(s) are particulate, so that the compositions are, for example, in tablet or powder form. The carrier(s) may be liquid, with the compositions being, for example, an oral oil, injectable liquid or an aerosol, which is useful in, for example, inhalatory administration. When intended for oral administration, the pharmaceutical composition is preferably in either solid or liquid form, where semi solid, semi liquid, suspension and gel forms are included within the forms considered herein as either solid or liquid.As a solid composition for oral administration, the pharmaceutical composition may be formulated into a powder, granule, compressed tablet, pill, capsule, chewing gum, wafer or the like. Such a solid composition will typically contain one or more inert diluents or edible carriers. In addition, one or more of the following may be present: binders such as carboxymethylcellulose, ethyl cellulose, microcrystalline cellulose, gum tragacanth or gelatin; excipients such as starch, lactose or dextrins, disintegrating agents such as alginic acid, sodium alginate, Primogel, corn starch and the like; lubricants such as magnesium stearate or Sterotex; glidants such as colloidal silicon dioxide; sweetening agents such as sucrose or saccharin; a flavoring agent such as peppermint, methyl salicylate or orange flavoring; and a coloring agent. When the composition is in the form of a capsule, for example, a gelatin capsule, it may contain, in addition to materials of the above type, a liquid carrier such as polyethylene glycol or oil.The composition may be in the form of a liquid, for example, an elixir, syrup, solution, emulsion or suspension. The liquid may be for oral administration or for WO 2021/226560 PCT/US2021/031442 delivery by injection, as two examples. When intended for oral administration, preferred compositions contain, in addition to the present compounds, one or more of a sweetening agent, preservatives, dye/colorant and flavor enhancer. In a composition intended to be administered by injection, one or more of a surfactant, preservative, wetting agent, dispersing agent, suspending agent, buffer, stabilizer and isotonic agent may be included.Liquid pharmaceutical compositions, whether they be solutions, suspensions or other like form, may include one or more of the following adjuvants: sterile diluents such as water for injection, saline solution, preferably physiological saline, Ringer ’s solution, isotonic sodium chloride, fixed oils such as synthetic mono or diglycerides which may serve as the solvent or suspending medium, polyethylene glycols, glycerin, propylene glycol or other solvents; antibacterial agents such as benzyl alcohol or methyl paraben; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic. Physiological saline is a preferred adjuvant. An injectable pharmaceutical composition is preferably sterile.A liquid composition intended for either parenteral or oral administration should contain an amount of an antibody or antigen-binding fragment as herein disclosed such that a suitable dosage will be obtained. Typically, this amount is at least 0.01% of the antibody or antigen-binding fragment in the composition. When intended for oral administration, this amount may be varied to be between 0.1 and about 70% of the weight of the composition. Certain oral pharmaceutical compositions contain between about 4% and about 75% of the antibody or antigen-binding fragment. In certain embodiments, pharmaceutical compositions and preparations according to the present invention are prepared so that a parenteral dosage unit contains between 0.01 to 10% by weight of antibody or antigen-binding fragment prior to dilution.

WO 2021/226560 PCT/US2021/031442 The composition may be intended for topical administration, in which case the carrier may suitably comprise a solution, emulsion, ointment or gel base. The base, for example, may comprise one or more of the following: petrolatum, lanolin, polyethylene glycols, bee wax, mineral oil, diluents such as water and alcohol, and emulsifiers and stabilizers. Thickening agents may be present in a composition for topical administration. If intended for transdermal administration, the composition may include a transdermal patch or iontophoresis device. The pharmaceutical composition may be intended for rectal administration, in the form, for example, of a suppository, which will melt in the rectum and release the drug. The composition for rectal administration may contain an oleaginous base as a suitable nonirritating excipient. Such bases include, without limitation, lanolin, cocoa butter and polyethylene glycol.A composition may include various materials which modify the physical form of a solid or liquid dosage unit. For example, the composition may include materials that form a coating shell around the active ingredients. The materials that form the coating shell are typically inert, and may be selected from, for example, sugar, shellac, and other enteric coating agents. Alternatively, the active ingredients may be encased in a gelatin capsule. The composition in solid or liquid form may include an agent that binds to the antibody or antigen-binding fragment of the disclosure and thereby assists in the delivery of the compound. Suitable agents that may act in this capacity include monoclonal or polyclonal antibodies, one or more proteins or a liposome. The composition may consist essentially of dosage units that can be administered as an aerosol. The term aerosol is used to denote a variety of systems ranging from those of colloidal nature to systems consisting of pressurized packages. Delivery may be by a liquefied or compressed gas or by a suitable pump system that dispenses the active ingredients. Aerosols may be delivered in single phase, bi phasic, or tri phasic systems in order to deliver the active ingredient(s). Delivery of the aerosol includes the necessary container, activators, valves, subcontainers, and the like, which together may form a kit. One of ordinary skill in the art, without undue experimentation, may determine preferred aerosols.

WO 2021/226560 PCT/US2021/031442 It will be understood that compositions of the present disclosure also encompass carrier molecules for polynucleotides, as described herein (e.g., lipid nanoparticles, nanoscale delivery platforms, and the like).The pharmaceutical compositions may be prepared by methodology well known in the pharmaceutical art. For example, a composition intended to be administered by injection can be prepared by combining a composition that comprises an antibody, antigen-binding fragment thereof, or antibody conjugate as described herein and optionally, one or more of salts, buffers and/or stabilizers, with sterile, distilled water so as to form a solution. A surfactant may be added to facilitate the formation of a homogeneous solution or suspension. Surfactants are compounds that non-covalently interact with the peptide composition so as to facilitate dissolution or homogeneous suspension of the antibody or antigen-binding fragment thereof in the aqueous delivery system.In general, an appropriate dose and treatment regimen provide the composition(s) in an amount sufficient to provide therapeutic and/or prophylactic benefit (such as described herein, including an improved clinical outcome (e.g., a decrease in frequency, duration, or severity of diarrhea or associated dehydration, or inflammation, or longer disease-free and/or overall survival, or a lessening of symptom severity). For prophylactic use, a dose should be sufficient to prevent, delay the onset of, or diminish the severity of a disease associated with disease or disorder. Prophylactic benefit of the compositions administered according to the methods described herein can be determined by performing pre-clinical (including in vitro and in vivo animal studies) and clinical studies and analyzing data obtained therefrom by appropriate statistical, biological, and clinical methods and techniques, all of which can readily be practiced by a person skilled in the art.Compositions are administered in an effective amount (e.g., to treat a coronavirus infection), which will vary depending upon a variety of factors including the activity of the specific compound employed; the metabolic 100 WO 2021/226560 PCT/US2021/031442 stability and length of action of the compound; the age, body weight, general health, sex, and diet of the subject; the mode and time of administration; the rate of excretion; the drug combination; the severity of the particular disorder or condition; and the subject undergoing therapy. In certain embodiments, tollowing administration of therapies according to the formulations and methods of this disclosure, test subjects will exhibit about a 10% up to about a 99% reduction in one or more symptoms associated with the disease or disorder being treated as compared to placebo-treated or other suitable control subjects.Generally, a therapeutically effective daily dose of an antibody or antigen binding fragment is (for a 70 kg mammal) from about 0.001 mg/kg (i.e., 0.07 mg) to about 100 mg/kg (i.e., 7.0 g); preferably a therapeutically effective dose is (for a 70 kg mammal) from about 0.01 mg/kg (i.e., 0.7 mg) to about 50 mg/kg (i.e., 3.5 g); more preferably a therapeutically effective dose is (for a 70 kg mammal) from about 1 mg/kg (i.e., 0ד mg) to about 25 mg/kg (i.e., 1.75 g). For polynucleotides, vectors, host cells, and related compositions of the present disclosure, a therapeutically effective dose may be different than for an antibody or antigen-binding fragment.In certain embodiments, a method comprises administering the antibody, antigen-binding fragment, polynucleotide, vector, host cell, or composition to the subject at 2, 3, 4, 5, 6, 7, 8, 9, 10 times, or more.In certain embodiments, a method comprises administering the antibody, antigen-binding fragment, or composition to the subject a plurality of times, wherein a second or successive administration is performed at about 6, about 7, about 8, about 9, about 10, about 11, about 12, about 24, about 48, about 74, about 96 hours, or more, following a first or prior administration, respectively.In certain embodiments, a method comprises administering the antibody, antigen-binding fragment, polynucleotide, vector, host cell, or composition at least one time prior to the subject being infected by SARS-C0V-2.Compositions comprising an antibody, antigen-binding fragment, polynucleotide, vector, host cell, or composition of the present disclosure may also be administered simultaneously with, prior to, or after administration of one or more other 101 WO 2021/226560 PCT/US2021/031442 therapeutic agents. Such combination therapy may include administration of a single pharmaceutical dosage formulation which contains a compound of the invention and one or more additional active agents, as well as administration of compositions comprising an antibody or antigen-binding fragment of the disclosure and each active agent in its own separate dosage formulation. For example, an antibody or antigen-binding fragment thereof as described herein and the other active agent can be administered to the patient together in a single oral dosage composition such as a tablet or capsule, or each agent administered in separate oral dosage formulations. Similarly, an antibody or antigen-binding fragment as described herein and the other active agent can be administered to the subject together in a single parenteral dosage composition such as in a saline solution or other physiologically acceptable solution, or each agent administered in separate parenteral dosage formulations. Where separate dosage formulations are used, the compositions comprising an antibody or antigen-binding fragment and one or more additional active agents can be administered at essentially the same time, i.e., concurrently, or at separately staggered times, i.e., sequentially and in any order; combination therapy is understood to include all these regimens.In certain embodiments, a combination therapy is provided that comprises one or more anti-SARS-C0V-2 antibody (or one or more nucleic acid, host cell, vector, or composition) of the present disclosure and one or more anti- inflammatory agent and/or one or more anti-viral agent. In particular embodiments, the one or more anti-inflammatory agent comprises a corticosteroid such as, for example, dexamethasone, prednisone, or the like. In some embodiments, the one or more anti-inflammatory agents comprise a cytokine antagonist such as, for example, an antibody that binds to IL6 (such as siltuximab), or to IL-6R (such as tocilizumab), or to IL-1p, IL-7, IL-8, IL-9, IL- 10, FGF, G-CSF, GM-CSF, IFN-y, IP-10, MCP-1, MIP-1A, MIP1-B, PDGR, TNF-a, or VEGF. In some embodiments, anti-inflammatory agents such as ruxolitinib and/or anakinra are used. In some embodiments, the one or more 102 WO 2021/226560 PCT/US2021/031442 anti-viral agents comprise nucleotide analogs or nucelotide analog prodrugs such as, for example, remdesivir, sofosbuvir, acyclovir, and zidovudine. In particular embodiments, an anti-viral agent comprises lopinavir, ritonavir, favipiravir, or any combination thereof. In some embodiments, a combination therapy comprises leronlimab. Anti- inflammatory agents for use in a combination therapy of the present disclosure also include non-steroidal anti-inflammatory drugs (NSAIDS). It will be appreciated that in such a combination therapy, the one or more antibody (or one or more nucleic acid, host cell, vector, or composition) and the one or more anti-inflammatory agent and/or one or the more antiviral agent can be administered in any order and any sequence, or together.In some embodiments, an antibody (or one or more nucleic acid, host cell, vector, or composition) is administered to a subject who has previously received one or more anti-inflammatory agent and/or one or more antiviral agent. In some embodiments, one or more anti-inflammatory agent and/or one or more antiviral agent is administered to a subject who has previously received an antibody (or one or more nucleic acid, host cell, vector, or composition).In certain embodiments, a combination therapy is provided that comprises two or more anti-SARS-CoV-2 antibodies, either or both of which can be antibodies of the present disclosure. A method can comprise administering a first antibody to a subject who has received a second antibody, or can comprise administering two or more antibodies together. For example, in particular embodiments, a method is provided that comprises administering to the subject (a) a first antibody or antigen-binding fragment, when the subject has received a second antibody or antigen-binding fragment; (b) the second antibody or antigen-binding fragment, when the subject has received the first antibody or antigen-binding fragment; or (c) the first antibody or antigen-binding fragment, and the second antibody or antigen-binding fragment.In some embodiments, any presently disclosed antibody can be used in a method of treating or preventing a SARS-C0V-2 infection, wherein the method further comprises use of an antibody or antigen-binding fragment that comprises: (i) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs. :343-345 and 347-349, respectively; or (ii) CDRH1, CDRH2, CDRH3, 103 WO 2021/226560 PCT/US2021/031442 CDRLl, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.: 140-142 and 144-146, respectively; or (iii) VH and VL amino acid sequences as set forth in SEQ ID NOs. :342 and 346, respectively; or (iv) VH and VL amino acid sequences as set forth in SEQ ID NOs. :139 and 143, respectively.In a related aspect, uses of the presently disclosed antibodies, antigen- binding fragments, vectors, host cells, and compositions are provided.In certain embodiments, an antibody, antigen-binding fragment, polynucleotide, vector, host cell, or composition is provided for use in a method of treating a SARS-CoV-2 infection in a subject.In certain embodiments, an antibody, antigen-binding fragment, or composition is provided for use in a method of manufacturing or preparing a medicament for treating a SARS-CoV-2 infection in a subject.

The present disclosure also provides the following Embodiments: Embodiment 1. An antibody, or antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRLl, a CDRL2, and a CDRL3, wherein:(i) the CDRH1 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 400, 23, 33, 43, 53, 63, 75, 85, 97, 107, 120, 130, 140, 147, 160, 170, 174, 183, 190, 199, 209, 219, 229, 241, 255, 265, 275, 285, 299, 313, 323, 333, 370, 380, 390, 410, 420, 430, 435, 445, 455, 465, 475, 485, 495, 505, 515, 525, 535, 545, 555, 565, 575, 585, 595, 605, 615, 631, and 693, or a sequence variant thereof comprising one, two, or three acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(ii) the CDRH2 comprises or consists of the amino acid sequence according to anyone of SEQ ID NOs.: 401, 24, 34, 44, 54, 64, 76, 86, 98, 108, 121, 131, 141, 148, 104 WO 2021/226560 PCT/US2021/031442 151, 161, 171, 184, 200, 210, 220, 230, 242, 256, 266, 276, 286, 300, 314, 324, 334,352, 360, 362, 364, 366, 371, 381, 391, 411, 421, 431, 436, 446, 456, 466, 476, 486,496, 506, 516, 526, 536, 546, 556, 566, 576, 586, 596, 606, 616, 625, 632, 635, 637,639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671,673, 675, 677, 679, 681, 683, 685, and 694, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(iii) the CDRH3 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 766, 25, 35, 45, 55, 65, 77, 87, 99, 109, 122, 132, 142, 149, 162, 164, 165, 172, 176, 177, 179, 180, 185, 187, 188, 201, 211, 221, 231, 243, 257,267, 277, 287, 301, 315, 325, 335, 354, 372, 382, 392, 412, 422, 432, 437, 447, 457,467, 477, 487, 497, 507, 517, 527, 537, 547, 557, 567, 577, 587, 597, 607, 617, 627,633, 695, 751, 753, 755, 757, 760, 763, 765, and 402, or a sequence variant thereofcomprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(iv) the CDRL1 comprises or consists of the amino acid sequence according to anyone of SEQ ID NOs.: 404, 27,37, 47, 57, 67, 79, 89, 101, 111, 124, 134, 144,152, 155, 156, 158, 159, 166, 181, 192, 203, 213, 223, 233, 245, 259, 269, 279, 289,303, 317, 327, 337, 356, 374, 384, 394, 414, 424, 439, 449, 459, 469, 479, 489, 499,509, 519, 529, 539, 549, 559, 569, 579, 589, 599, 609, 619, 687, and 697, or a sequencevariant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(v) the CDRL2 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 405, 28, 38, 48, 58, 68, 80, 90, 102, 112, 125, 135, 145, 153, 167, 182, 193, 204, 214, 224, 234, 246, 260, 270, 280, 290, 304, 318, 328, 338, 375, 385, 395, 415, 425, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 688, and 698, or a sequence variant thereof 105 WO 2021/226560 PCT/US2021/031442 compnsing one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; and/or(vi) the CDRL3 comprises or consists of the amino acid sequence according to anyone of SEQ ID NOs : 406, 29,39, 49, 59, 69,81,91, 103, 113, 126, 136, 146,169, 195, 197, 205, 215, 225, 235, 247, 261, 271, 281, 291, 305, 319, 329, 339, 358,376, 386, 396, 416, 426, 441, 451, 461, 471, 481, 491, 501, 511, 521, 531, 541, 551,561, 571, 581, 591, 601, 611, 621, 689, 699, 745 and 747, or a sequence variant thereofcomprising having one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 2. The antibody or antigen-binding fragment of Embodiment!, which is capable of neutralizing a SARS-CoV-2 infection in an in vitro model of infection and/or in an in vivo animal model of infection and/or in a human.

Embodiment 3. The antibody or antigen-binding fragment of any one of Embodiments 1-2, comprising CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences of SEQ ID NOs.: (i) 400, 401, 766, and 404-406, respectively; (ii)400-402 and 404-406, respectively; (iii) 43-45 and 47-49, respectively; (iv) 53-55 and 57-59, respectively; (v) 63-65 and 67-69, respectively; (vi) 75-77 and 79-81, respectively; (vii) 85-87 and 89-91, respectively; (viii) 97-99 and 101-103, respectively; (ix) 107-109 and 111-113, respectively; (x) 120-122 and 124-126, respectively; (xi) 130-132 and 134-136, respectively; (xii) 23 or 147, any one of 24, 1or 151, 25 or 149, any one of 27, 152, 155, 156, 158, or 159, 28 or 153, and 29, respectively; (xiii) 43 or 160, 44 or 161, any one of 45, 162, 164, or 165, 47 or 166, or 167, and 49 or 169, respectively; (xiv) any one of 130, 170, or 174, 130, 131, 132, 106 WO 2021/226560 PCT/US2021/031442 134 or 181, 135 or 182, and 136, respectively; (xv) any one of 53, 183, or 190, 54 or 184, any one of 55, 185, 187, or 188, 57 or 192, 58 or 193, and any one of 59, 195, or 197, respectively; (xvi) 199-201 and 203-205, respectively; (xvii) 209-211 and 213- 215, respectively; (xviii) 219-221 and 223-225, respectively; (xix) 229-231 and 233- 235, respectively; (xx) 241-243 and 245-247, respectively; (xxi) 255-257 and 259-261, respectively;(xxii) 265-267 and 269-271, respectively; (xxiii) 275-277 and 279-281, respectively; (xxiv) 285-287 and 289-291, respectively; (xxv) 299-301 and 303-305, respectively; (xxvi) 313-315 and 317-319, respectively; (xxvii) 323-325 and 327-329, respectively; (xxviii) 333-335 and 337-339, respectively; (xxix) 229, 230 or 352, 231 or 354, and 233 or 356, 234, and 235 or 358, respectively; (xxx) 313, any one of 314, 360, 362, 364, or 366, 315 and 317-319, respectively; (xxxi) 370-372 and 374-376, respectively; (xxxii) 380-382 and 384-386, respectively; (xxxiii) 390-392 and 394-396, respectively; (xxxiv)23-25 and 27-29, respectively; (xxxv) 410-412 and 414-416, respectively; (xxxvi) 420-422 and 424-426, respectively; (xxxvii) 435-437 and 439- 441, respectively; (xxxviii) 445-447 and 449-451, respectively; (xxxix) 455-457 and 459-461, respectively; (xxxx) 465-467 and 469-471, respectively; (xxxxi) 475-477 and 479-481, respectively; (xxxxii) 485-487 and 489-491, respectively; (xxxxiii) 494-4and 499-501, respectively; (xxxxiv) 505-507 and 509-511, respectively; (xxxxv) 515- 517 and 519-521, respectively; (xxxxvi) 525-527 and 529-531, respectively; (xxxxvii) 535-537 and 539-541, respectively; (xxxxviii) 545-547 and 549-551, respectively;(xxxxix) 555-557 and 559-561, respectively; (xxxxx) 565-567 and 569-571, respectively; (xxxxxi) 575-577 and 579-581, respectively; (xxxxxii) 585, 586 or 625, 587 or 627, and 589-591, respectively; (xxxxxiii) 595-597 and 599-601, respectively; (xxxxxiv) 605-607 and 609-611, respectively; (xxxxxv) 615-617 and 619-621, respectively; (xxxxxvi) 631, 632 or 635 or 637 or 639 or 641 or 643 or 645 or 647 or 649 or 651 or 653 or 655 or 657 or 659 or 661 or 663 or 665 or 667 or 669 or 671 or 673 or 675 or 677 or 679 or 681 or 683 or 685, 633, and 697-699, respectively;(xxxxxvii) 693-695 and 697-699, respectively; (xxxxxviii) 400, 401 and any one of 751, 753, 755, 757, or 760 and 404, 405, and any one of 745 or 747, respectively; (xxxxxxix) 585, 586, and 762 or 764 and 589-591, respectively; (xxxxxxx) 33-35 and 107 WO 2021/226560 PCT/US2021/031442 37-39, respectively; or (xxxxxxxi) 400, 401, 766, 404, 405 or variant of 405 comprising one, two, or three amino acid substiutions, wherein each of the one, two, or three amino acid substitutions is optionally a conservative amino acid substitution, and 406, respectively.

Embodiment 4. An antibody, or antigen-binding fragment thereof, comprising the CDRH1, the CDRH2, and the CDRH3 of the VH amino acid sequence set forth in SEQ ID NO. :399, and the CDRL1, the CDRL2 or a variant of the CDRLcomprising one, two, or three amino acid substiutions, wherein each of the one, two, or three amino acid substitutions is optionally a conservative amino acid substitution, and the CDRL3 of the VL amino acid sequence set forth in SEQ ID NO.:738, wherein the CDRs are according to IMGT, and wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 5. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in: (a) SEQ ID NOs.:400, 401, 766, 404, 405, and 406, respectively; (b) SEQ ID NOs.:400, 401, 769, 404, 405, and 406, respectively; (c) SEQ ID NOs.:400, 401, 770, 404, 405, and 406, respectively; (d) SEQ ID NOs.:400, 401, 771, 404, 405, and 406, respectively; (e) SEQ ID NOs.:400, 401, 772, 404, 405, and 406, respectively; (f) SEQ ID NOs.:400, 401, 773, 404, 405, and 406, respectively; (g) SEQ ID NOs.:400, 401, 766, 404, 405, and 745, respectively; (h) SEQ ID NOs.:400, 401, 769, 404, 405, and 745, respectively; (i) SEQ ID NOs.:400, 401, 770, 404, 405, and 745, respectively; (j) SEQ ID NOs.:400, 401, 771, 404, 405, and 745, respectively; (k) SEQ ID NOs.:400, 401, 772, 404, 405, and 745, respectively; (1) SEQ ID NOs.: 400, 401, 773, 405, 405, and 745, respectively; 108 WO 2021/226560 PCT/US2021/031442 (m) SEQ ID NOs. :400, 401, 766, 404, 405, and 747, respectively; (n) SEQ ID NOs. :400, 401, 769, 404, 405, and 747, respectively; (o) SEQ ID NOs. :400, 401, 770, 404, 405, and 747, respectively; (p) SEQ ID NOs. :400, 401, 771, 404, 405, and 747, respectively; (q) SEQ ID NOs. :400, 401, 772, 404, 405, and 747, respectively; or (r) SEQ ID NOs. :400, 401, 773, 404, 405, and 747, respectively, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 6. The antibody or antigen-binding fragment thereof of Embodiment 5, comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 766, 404, 405, and 406, respectively.

Embodiment 7. The antibody or antigen-binding fragment of any one ofEmbodiments 4-6, comprising the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 402, 404, 405, and 406; (b) SEQ ID NOs. :400, 401, 751, 404, 405, and 406; (c) SEQ ID NOs. :400, 401, 753, 404, 405, and 406; (d) SEQ ID NOs.:400, 401, 755, 404, 405, and 406; (e) SEQ ID NOs.:400, 401, 757, 404, 405, and 406; (f) SEQ ID NOs. :400, 401, 760, 404, 405, and 406; (g) SEQ ID NOs. :400, 401, 402, 404, 405, and 745; (h) SEQ ID NOs.:400, 401, 751, 404, 405, and 745; (i) SEQ ID NOs.:400, 401, 753, 404, 405, and 745; (j) SEQ ID NOs.:400, 401, 755, 404, 405, and 745; (k) SEQ ID NOs. :400, 401, 757, 404, 405, and 745; (1) SEQ ID NOs.: 400, 401, 760, 404, 405, and 745; (m) SEQ ID NOs. :400, 401, 402, 404, 405, and 747; (n) SEQ ID NOs. :400, 401, 751, 404, 405, and 747; (o) SEQ ID NOs.:400, 401, 753, 404, 405, and 747; (p) SEQ ID NOs. :400, 401, 755, 404, 405, and 747; (q) SEQ ID NOs.:400, 401, 757, 404, 405, and 747; or (r) SEQ ID NOs. :400, 401, 760, 404, 405, and 747. 109 WO 2021/226560 PCT/US2021/031442 Embodiment 8. The antibody or antigen-binding fragment of any one of Embodiments 4-7, comprising, in VH, the amino acid sequence set forth in SEQ ID NO.: 400, the amino acid sequence set forth in SEQ ID NO.:401, and the amino acid sequence set forth in SEQ ID NO. :402, and in VL, the amino acid sequence set forth in SEQ ID NO. :404, the amino acid sequence set forth in SEQ ID NO. :405, and the amino acid sequence set forth in SEQ ID NO. :406.

Embodiment 9. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in SEQ ID NOs.:525, 526, 527, 529, 530, and 531, respectively, and wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 10. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in SEQ ID NOs.:585, 586 or 625, 587 or 627, 589, 590, and 591, respectively, and wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 11. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain 110 WO 2021/226560 PCT/US2021/031442 (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in SEQ ID NOs.:229, 230, 231, 233, 234, and 235, respectively,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 12. The antibody or antigen-binding fragment of any one of Embodiments 1-11, wherein:(i) the VH comprises or consists of an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence according to any one of SEQ IDNOs.: 399, 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 254, 264, 274, 284, 298, 312, 322, 332,350, 351, 353, 359, 361, 363, 365, 367, 368, 369, 379, 389, 409, 419, 429, 434, 444,454, 464, 474, 484, 494, 504, 514, 524, 534, 544, 554, 564, 574, 584, 594, 604, 614,624, 626, 628, 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658,660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 692, 740, 741, 742,743, 748, 749, 750, 752, 754, 756, 758, 759, 761, 762, and 764, wherein the variation is optionally limited to one or more framework regions and/or the variation comprises one or more substitution to a germline-encoded amino acid; and/or(ii) the VL comprises or consists of an amino acid sequence having at least 85% (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence according to any one of SEQ IDNOs.: 738, 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133, 143, 154, 157, 168,194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278, 288, 294, 296, 302,308, 310, 316, 326, 336, 355, 357, 373, 383, 393, 403, 413, 423, 438, 448, 458, 468,478, 488, 498, 508, 518, 528, 538, 548, 558, 568, 578, 588, 598, 608, 618, 686, 696,744, and 746, wherein the variation is optionally limited to one or more framework 111 WO 2021/226560 PCT/US2021/031442 regions and/or the variation comprises one or more substitution to a germhne-encoded amino acid.

Embodiment 13. The antibody or antigen-binding fragment of any one of Embodiments 1-12, wherein the VH comprises or consists of an amino acid sequence having at least 85% identity to the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of an amino acid sequence having at least 85% identity to the amino acid sequence set forth in SEQ ID NO. :403 or SEQ ID NO.:738.

Embodiment 14. The antibody or antigen-binding fragment of any one of Embodiments 1-13, wherein the VH comprises or consists of an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

Embodiment 15. The antibody or antigen-binding fragment of any one of Embodiments 1-14, wherein the VH comprises or consists of an amino acid sequence having at least 95% identity to the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of an amino acid sequence having at least 95% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

Embodiment 16. The antibody or antigen-binding fragment of any one of Embodiments 1-15, wherein the VH comprises or consists of an amino acid sequence having at least 97% identity to the amino acid sequence set forth in SEQ ID NO. :399 and the VL comprises or consists of an amino acid sequence having at least 97% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738. 112 WO 2021/226560 PCT/US2021/031442 Embodiment 17. The antibody or antigen-binding fragment of any one of claims 1-16, wherein the VH comprises or consists of an amino acid sequence having at least 99% identity to the amino acid sequence set forth in SEQ ID NO.:399 and the VL comprises or consists of an amino acid sequence having at least 99% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

Embodiment 18. The antibody or antigen-binding fragment of any one of Embodiments 1-12, wherein the VH and the VL have at least 85% identity (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) to the amino acid sequences set forth in:(i) SEQ ID NOs.:524 and 528, respectively;(ii) SEQ ID NOs.:584 or 624 or 626 or 628 and 588, respectively;(iii) SEQ ID NOs.:228 or 740 or 741 or 742 or 743 and 232, respectively; or(iv) SEQ ID NOs.:228 or 740 or 741 or 742 or 743 and 238, respectively.

Embodiment 19. The antibody or antigen-binding fragment of any one of Embodiments 1-18, wherein the VH comprises or consists of any VH amino acid sequence set forth in Table 2, and wherein the VL comprises or consists of any VL amino acid sequence set forth in Table 2, wherein, optionally, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.: (i) 399 and 403 or 738, respectively; (ii) 32 and 36, respectively; (iii) 42 and 46, respectively; (iv) and 56, respectively; (v) 62 and 66, respectively; (vi) 72 and 66, respectively; (vii) and 78, respectively; (viii) 84 and 88, respectively; (ix) 84 and 88, respectively; (x) 96 and 100, respectively; (xi) 106 and 110, respectively; (xii) 119 and 123, respectively; or (xiii) 129 and 133, respectively; (xiv) 22 or 150 and 26, 154, or 157, respectively; (xv) 42 or 163 and 46 or 168, respectively; (xvi) any one of 129, 173, 175, or 178 and 133, respectively; (xvii) any one of 52, 186, 189, or 191 and any one of 56, 194, or 196, respectively; (xviii) 198 and 202, respectively; (xix) 208 and 212, respectively; (xx) 218 and 222, respectively; (xxi) 228 and 232 or 238, respectively; 113 WO 2021/226560 PCT/US2021/031442 (xxn) 240 and any one of 244, 250, or 252, respectively; (xxm) 254 and 258, respectively; (xxiv) 264 and 268, respectively; (xxv) 274 and 278, respectively; or (xxvi) 284 and any one of 288, 294, or 296, respectively; (xxvii) 298 and any one of 302, 308, or 310, respectively; (xxviii) 312 and 316, respectively; (xxix) 322 and 326, respectively; (xxx) 332 and 336, respectively; (xxxi) any one of 228, 350, 351, or 3and 232, 238, 355, or 357, respectively; (xxxii) any one of 312, 359, 361, 363, 365, 367, or 368 and 316, respectively; (xxxiii) 369 and 373, respectively; (xxxiv) 379 and 383, respectively; (xxxv) 389 and 393, respectively; (xxxvi) 22 and 26, respectively; (xxxvii) 409 and 413, respectively; (xxxviii) 419 and 423, respectively; (xxxix) 434 and 438, respectively; (xxxx) 444 and 448, respectively; (xxxxi) 454 and 458, respectively;(xxxxii) 464 and 468, respectively; (xxxxiii) 474 and 478, respectively; (xxxxiv) 4and 488, respectively; (xxxxv) 494 and 498, respectively; (xxxxvi) 504 and 508, respectively; (xxxxvii) 514 and 518, respectively; (xxxxviii) 524 and 528, respectively; (xxxxix) 534 and 538, respectively; (xxxxx) 544 and 548, respectively; (xxxxxi) 5and 558, respectively; (xxxxxii) 564 and 568, respectively; (xxxxxiii) 574 and 578, respectively; (xxxxxiv) 584 and 588, respectively; (xxxxxv) 594 and 598, respectively; (xxxxxvi) 604 and 608, respectively; (xxxxxvii) 614 and 618, respectively; (xxxxxviii) 624, 626, or 628 and 588, respectively; (xxxxxix) 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, or 684, and 686, respectively; (xxxxxx) 692 and 696, respectively; (xxxxxxi) any one of 740-743 and 238, respectively; (xxxxxxii) any one of 399, 748, 749, 750, 752, 754, 756, 758, 759, and 761 and any one of 403, 744, and 746, respectively; or (xxxxxxiii) 762 or 764 and 588, respectively.

Embodiment 20. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO:399 and the VL comprises or consists of the amino acid sequence set forth in SEQIDNO:738, 114 WO 2021/226560 PCT/US2021/031442 wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 21. An antibody, or an antigen-binding fragment thereof,comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO:399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:403, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 22. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:403, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 23. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:738, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion. 115 WO 2021/226560 PCT/US2021/031442 Embodiment 24. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:744, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 25. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:746, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 26. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO:524 and the VL comprises or consists of the amino acid sequence set forth in SEQIDNO:528,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 27. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any 116 WO 2021/226560 PCT/US2021/031442 one of SEQ ID NOs.:584, 624, 626, and 628 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:588,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 28. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs.:228, 740, 741, 742, and 743, and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:232,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 29. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs.:228, 740, 741, 742, and 743, and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:238,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

Embodiment 30. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence as set forth in SEQ ID NO: 32 and the VL comprises or consists of the amino acid sequence as set forth in SEQ ID NO: 36. 117 WO 2021/226560 PCT/US2021/031442 Embodiment 31. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 33-35, respectively, and the CDRLI, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 37-39, respectively.

Embodiment 32. The antibody or antigen-binding fragment of any one of Embodiments 3-31, which is capable of neutralizing a SARS-CoV-2 infection in an in vitro model of infection and/or in an in vivo animal model of infection and/or in a human.

Embodiment 33. The antibody or antigen-binding fragment of any one of Embodiments 1-32, which:(i) recognizes an epitope in the ACE2 receptor binding motif (RBM, SEQ ID NO. :5) of SARS-CoV-2;(ii) is capable of blocking an interaction between SARS-CoV-2 (e.g., SARS- C0V-2 RBM) and human ACE2;(iii) is capable of binding to SARS-C0V-2S protein;(iv) recognizes an epitope that is conserved in the ACE2 RBM of SARS- C0V-2 and in an ACE2 RBM of SARS-CoV-1;(v) is cross-reactive against SARS-CoV-2 and SARS-CoV-1 coronavirus;(vii) recognizes an epitope in the SARS-CoV-2 surface glycoprotein that is not in the ACE2 RBM;(viii) is capable of binding to a SARS-CoV-2 S protein trimer in a prefusion conformation;or(ix) any combination of (i)-(viii). 118 WO 2021/226560 PCT/US2021/031442 Embodiment 34. The antibody or antigen-binding fragment of any one of Embodiments 1-33, which is a IgG, IgA, IgM, IgE, or IgD isotype.

Embodiment 35. The antibody or antigen-binding fragment of any one of Embodiments 1-34, which is an IgG isotype selected from IgGl, IgG2, IgG3, and IgG4.

Embodiment 36. The antibody or antigen-binding fragment of any one of Embodiments 1-35, which is human, humanized, or chimeric.

Embodiment 37. The antibody or antigen-binding fragment of any one of Embodiments 1-36, wherein the antibody, or the antigen-binding fragment, comprises a human antibody, a monoclonal antibody, a purified antibody, a single chain antibody, a Fab, a Fab ’, a F(ab ’)2, a Fv, a scFv, or a scFab.

Embodiment 38. The antibody or antigen-binding fragment of Embodiment 37, wherein the scFv comprises more than one VH domain and more than one VL domain.

Embodiment 39. The antibody or antigen-binding fragment of any one of Embodiments 1-38, wherein the antibody or antigen-binding fragment is a multi-specific antibody or antigen binding fragment.

Embodiment 40. The antibody or antigen-binding fragment of Embodiment 39, wherein the antibody or antigen binding fragment is a bispecific antibody or antigen-binding fragment.

Embodiment 41. The antibody or antigen-binding fragment of Embodiment or 40, comprising:(i) a first VH and a first VL; and(ii) a second VH and a second VL, 119 WO 2021/226560 PCT/US2021/031442 wherein the first VH and the second VH are different and each independently comprise an amino acid sequence having at least 85% (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 254, 264, 274, 284, 298, 312, 322, 332, 350, 351, 353, 359, 361, 363, 365, 367,368, 369, 379, 389, 399, 409, 419, 429, 434, 444, 454, 464, 474, 484, 494, 504, 514,524, 534, 544, 554, 564, 574, 584, 594, 604, 614, 624, 626, 628, 630, 634, 636, 638,640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672,674, 676, 678, 680, 682, 684, 692, 740, 741, 742, 743, 748, 749, 750, 752, 754, 756,758, 759, 761, 762, and 764, andwherein the first VL and the second VL are different and each independently comprise an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133, 143, 154, 157, 168, 194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278, 288, 294, 296, 302, 308, 310, 316, 326, 336, 355, 357, 373, 383, 393, 403, 413, 423, 438, 448, 458, 468, 478, 488, 498, 508, 518, 528, 538, 548, 558, 568, 578, 588, 598, 608, 618, 686, 696 738, 744, and 746;and wherein the first VH and the first VL together form a first antigen-binding site, and wherein the second VH and the second VL together form a second antigen- binding site.

Embodiment 42. The antibody or antigen-binding fragment of Embodiment or 41, comprising:(i) a first VH and a first VL; and(ii) a second VH and a second VL,wherein the first VH comprises an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID 120 WO 2021/226560 PCT/US2021/031442 NOs.: 139 and 342 and the first VL comprises an amino acid sequence having at least 85% (i.e., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 143 and 346 and wherein the second VH comprises an amino acid sequence having at least 85% (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 399, 748, 749, 750, 752, 754, 756, 758, 759, and 761 and the second VL comprises an amino acid sequence having at least 85% (i.e., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs. :403, 744, and 746.

Embodiment 43. The antibody or antigen-binding fragment of Embodiment or 40, comprising a first antigen-binding portion having a first specificity and a second antigen-binding portion having a second specificity, wherein the first antigen- binding portion comprises a VH that comprises or consists of the amino acid sequence set forth in SEQ ID NO:399 and a VL that comprises or consists of the amino acid sequence set forth in SEQ ID NO.:738 or SEQ ID NO.:403.

Embodiment 44. The antibody or antigen-binding fragment of Embodiment 43, wherein the second antigen-binding portion comprises a VH that comprises or consists of the amino acid sequence set forth in SEQ ID NO.: 139 and a VL that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :143.

Embodiment 45. The antibody or antigen-binding fragment of Embodiment 43, wherein the second antigen-binding portion comprises a VH that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :342 and a VL that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :346. 121 WO 2021/226560 PCT/US2021/031442 Embodiment 46. The antibody or antigen-binding fragment of any one of Embodiments 1-45, wherein the antibody or antigen-binding fragment further comprises a Fc polypeptide or a fragment thereof.

Embodiment 47. The antibody or antigen-binding fragment of Embodiment 46, wherein the Fc polypeptide or fragment thereof comprises:(i) a mutation that enhances binding to a FcRn as compared to a reference Fc polypeptide that does not comprise the mutation; and/or(ii) a mutation that enhances binding to a FcyR as compared to a reference Fc polypeptide that does not comprise the mutation.

Embodiment 48. The antibody or antigen-binding fragment of Embodiment 47, wherein the mutation that enhances binding to a FcRn comprises: M428L; N434S; N434H; N434A; N434S; M252Y; S254T; T256E; T250Q; P257I; Q31II; D376V; T307A; E380A; or any combination thereof.

Embodiment 49. The antibody or antigen-binding fragment of Embodiment or 48, wherein the mutation that enhances binding to FcRn comprises: (i) M428L/N434S; (ii) M252Y/S254T/T256E; (iii) T250Q/M428L; (iv) 257I/Q31II; (v) P2571/N434H; (vi) D376V/N434H; (vii) T307A/E380A/N434A; or (viii) any combination of (i)-(vii).

Embodiment 50. The antibody or antigen-binding fragment of any one of Embodiments 47-49, wherein the mutation that enhances binding to FcRn comprises M428L/N434S.

Embodiment 51. The antibody or antigen-binding fragment of any one of Embodiments 47-50, wherein the mutation that enhances binding to a FcyR comprises S239D; I332E; A330L; G236A; or any combination thereof. 122 WO 2021/226560 PCT/US2021/031442 Embodiment 52. The antibody or antigen-binding fragment of any one of Embodiments 47-51, wherein the mutation that enhances binding to a FcyR comprises: (i) S239D/I332E; (ii) S239D/A330L/I332E; (iii) G236A/S239D/I332E; or(iv) G236A/A330L/I332E.

Embodiment 53. The antibody or antigen-binding fragment of any one of Embodiments 47-52, wherein the Fc polypeptide comprises a L234A mutation and a L235A mutation.

Embodiment 54. The antibody or antigen-binding fragment of any one of Embodiments 1-53, which comprises a mutation that alters glycosylation, wherein the mutation that alters glycosylation comprises N297A, N297Q, or N297G, and/or which is aglycosylated and/or afucosylated.

Embodiment 55. The antibody or antigen-binding fragment of any one of Embodiments 1-54, which is capable of binding to a SARS-CoV-2 surface glycoprotein with an EC50 of less than 500 ng/ml, less than 250 ng/ml, less than 100 ng/ml, less than ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than ng/ml, less than 15 ng/ml, less than 14 ng/ml, less than 13 ng/ml, less than 12 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than ng/ml, less than 5 ng/ml, less than 4 ng/ml, or less than 2 mg/ml, as measured by ELISA (optionally, indirect ELISA and/or sandwich ELISA) and/or by flow cytometry, wherein the SARS C0V-2 surface glycoprotein is expressed at a cell surface of a host cell.

Embodiment 56. The antibody or antigen-binding fragment of any one of Embodiments 1-55, which is capable of binding to a SARS-CoV-2 surface glycoprotein RED with an EC50 of less than 500 ng/ml, less than 250 ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 123 WO 2021/226560 PCT/US2021/031442 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 16 ng/ml, less than 15 ng/ml, less than 14 ng/ml, less than 13 ng/ml, less than ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, or less than 2 mg/ml, as measured by ELISA (optionally, indirect ELISA and/or sandwich ELISA) and/or by flow cytometry, wherein the SARS C0V-2 surface glycoprotein is expressed at a cell surface of a host cell.

Embodiment 57. The antibody or antigen-binding fragment of any one of Embodiments 1-56, which is capable of binding to a SARS-CoV-2 RED with a KD of less than 5 x 108־ M, less than 4 x 108־ M, less than 3 x 108־ M, less than 2 x 108־ M, less than 1 x 108־ M, less than 5 x 109־ M, less than 1 x 109־ M, less than 5 x 1010־ M, less than 1 x 1010־ M, less than 5 x 1011־ M, less than 1 x 1011־ M, less than 5 x 1012־ M, or less than 1 x 1012־M, as determined using biolayer interferometry (BLI), optionally using an Octet instrument with antibody or antigen-binding fragment loaded on Protein A pins, optionally at 2.7 ug/ml, and SARS-CoV-2 RBD loaded for 5 minutes at ug/ml, 1.5 ug/ml, or 0.4 ug/ml, further optionally with dissociation measured for minutes.

Embodiment 58. The antibody or antigen-binding fragment of any one of Embodiments 1-57, which is capable of binding to a SARS-CoV-2 RBD with a KD of less than 6 x 108־ M, less than 5 x 108־ M, less than 4 x 108־ M, less than 3 x 108־ M, less than 2 x 108־ M, less than 1 x 108־ M, less than 5 x 109־ M, less than 4 x 109־ M, less than 3 x 109־ M, less than 2 x 109־ M, less than 1 x 109־ M, or less than 8 x 1010־ M, as determined using surface plasmon resonance (SPR), optionally using a Biacore T2instrument using a single-cycle kinetics approach.

Embodiment 59. The antibody or antigen-binding fragment of any one of claims 1-58, which is capable of binding to a SARS-CoV-2 RBD and inhibiting an interaction between (i) the RBD and (ii) a human ACE2 and/or a human SIGLEC-1. 124 WO 2021/226560 PCT/US2021/031442 Embodiment 60. The antibody or antigen-binding fragment of any one of Embodiments 1-59, which is capable of neutralizing:(i) infection by a SARS-CoV-2 pseudovirus, optionally:(i)(a) with a neutralization IC50 of less than 100 ng/ml, less than ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20ng/ml, less than 15 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml,less than 3 ng/ml, less than 2 ng/ml, or less than 1 ng/ml, preferably less than ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, less than 3 ng/ml, less than 2 ng/ml, or less than 1 ng/ml, and/or(i)(b) with a neutralization IC80 of less than 100 ng/ml, less than ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than ng/ml, less than 40 ng/ml, less than 30 ng/ml, or less than 25 ng/ml, preferably less than 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, or less than 25 ng/ml, and/or(i)(c) with a neutralization EC90 of less than 300 ng/ml, less than 2ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than ng/ml, less than 60 ng/ml 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, or less than 10 ng/ml, wherein, further optionally, the SARS-CoV-2 pseudovirus comprises a VSV pseudovirus and/or a MEV pseudovirus, and/or(i)(d) the SARS-CoV-2 pseudovirus comprises a VSV pseudovirus and/or a MEV pseudovirus; and/or(ii) infection by live SARS-CoV-2, optionally(ii)(a) with a EC50 of less than 60 ng/ml, less than 50 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or 125 WO 2021/226560 PCT/US2021/031442 less than 4 ng/ml, preferably less than 15 ng/ml, less than 12 ng/ml, less than ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, and/or(ii)(b) with a EC90 of less than 50 ng/ml, less than 40 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, preferably less than 30 ng/ml, less than 25 ng/ml, less than ng/ml, less than 15 ng/ml, or less than 12 ng/ml, and/or(ii)(c) over a 6-hour period, with a multiplicity of infection of 0.1; and/or (iii) infection by live SARS-CoV-2 in a host cell (e.g. a HEK293T cell) that expresses, optionally is engineered to overexpress, DC-SIGN, L-SIGN, SIGLEC, or ACE2; and/or(iv) infection by live SARS-CoV-2 in a host cell (e.g. a HEK293T cell) that expresses, optionally is engineered to overexpress, SIGLEC-1 or ACE2, wherein neutralizing infection comprises fully neutralizing infection.

Embodiment 61. The antibody or antigen-binding fragment of any one of Embodiments 1-60, which is capable of neutralizing infection by a SARS-CoV-variant that comprises any one of the following mutations in the surface glycoprotein as compared to a SARS-CoV-2 surface glycoprotein comprising SEQ ID NO.:3: N501Y; S477N; N439K; L452R; E484K; K417N; T478K; S494P; A520S; N501T; A522S; Y453F; P384L.

Embodiment 62. The antibody or antigen-binding fragment of Embodiment 61, which is capable of neutralizing infection by the SARS-CoV-2 variant with a potency that is less than 3-fold lower than the potency with which the antibody or antigen-binding fragment neutralizes infection by a SARS-CoV-2 comprising the surface glycoprotein amino acid sequence set forth in SEQ ID NO. :3. 126 WO 2021/226560 PCT/US2021/031442 Embodiment 63. The antibody or antigen-binding fragment of any one of Embodiments 1-62, which is capable of activating a FcyRIIa, a FcyRIIIa, or both, wherein, optionally:(!)the FcyRIIa comprises a H131 allele; and/or(ii) the FcyRIIIa comprises a VI58 allele; and/or(iii) activation is determined using a SARS-C0V-2 S-expressing target cell, such as a CHO cell, and a reporter cell expressing a NFAT-driven reporter, such as luciferase.

Embodiment 64. The antibody or antigen-binding fragment of any one of Embodiments 1-63, comprising:(a) the CH1-CH3 amino acid sequence set forth in SEQ ID NO. :6 and the CL amino acid sequence set forth in SEQ ID NO. :8;(b) The CH1-CH3 amino acid sequence set forth in SEQ ID NO.:6 and the CL amino acid sequence set forth in SEQ ID NO.:9;(c) the CH1-CH3 amino acid sequence set forth in SEQ ID NO. :7 and the CL amino acid sequence set forth in SEQ ID NO. :8; or(d) The CH1-CH3 amino acid sequence set forth in SEQ ID NO.:7 and the CL amino acid sequence set forth in SEQ ID NO.:9.

Embodiment 65. An isolated antibody comprising:(i) the heavy chain amino acid sequence set forth in SEQ ID NO. :767; and (ii) the light chain amino acid sequence set forth in SEQ ID NO.:768.

Embodiment 66. The antibody or antigen-binding fragment of any one of Embodiments 1-65, which has an in vivo half-life in a non-human primate of between and 30 days, or between 22 and 28 days, or between 23 and 27 days, or between and 26 days, or of about 25 days.

Embodiment 67. The antibody or antigen-binding fragment of any one of Embodiments 1-66, wherein the antibody or antigen-binding fragment is capable of 127 WO 2021/226560 PCT/US2021/031442 neutralizing a SARS-CoV-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 20 to about 30 ng/ml.

Embodiment 68. The antibody or antigen-binding fragment of any one of Embodiments 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS-CoV-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 10 to about 20 ng/ml.

Embodiment 69. The antibody or antigen-binding fragment of any one of Embodiments 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS-CoV-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 5 to about 10 ng/ml.

Embodiment 70. The antibody or antigen-binding fragment of any one of Embodiments 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS-CoV-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 1 to about 5 ng/ml.

Embodiment 71. The antibody or antigen-binding fragment of any one of Embodiments 1-70, wherein the antibody or antigen-binding fragment is capable of neutralizing infection by SARS-CoV-2 and does not compete with a human ACE2 for binding to the SARS-CoV-2S protein, wherein, optionally, the neutralizing comprises neutralizing infection in an in vitro model of infection.

Embodiment 72. An antibody, or an antigen-binding fragment thereof, that competes for binding to a SARS-CoV-2 surface glycoprotein with the antibody or antigen-binding fragment of any one of Embodiments 1-71. 128 WO 2021/226560 PCT/US2021/031442 Embodiment 73. An isolated polynucleotide encoding the antibody or antigen-binding fragment of any one of Embodiments 1-72, or encoding a VH, a heavy chain, a VL, and/or a light chain of the antibody or the antigen-binding fragment.

Embodiment 74. The polynucleotide of Embodiment73, wherein the polynucleotide comprises deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), wherein the RNA optionally comprises messenger RNA (mRNA).

Embodiment 75. The polynucleotide of Embodiment73 or 74, which is codon-optimized for expression in a host cell.

Embodiment 76. The polynucleotide of any one of Embodiments 73-75, comprising a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or comprises or consists of, the polynucleotide sequence according to any one or more of SEQ ID NOs.: 30, 31, 40, 41, 50, 51, 60, 61, 70, 71, 73, 82, 83, 92, 93, 95, 104, 105, 114, 115, 116, 117, 118, 127, 128, 137, 138, 206, 207, 216, 217, 226, 227, 236, 237, 239, 248, 249, 251, 253, 262, 263, 272, 273,282, 283, 292, 293, 295, 297, 306, 307, 309, 311, 320, 321, 330, 331, 340, 341, 377,378, 387, 388, 397, 398, 407, 408, 417, 418, 427, 428, 433, 442, 443, 452, 453, 462,463, 472, 473, 482, 483, 492, 493, 502, 503, 512, 513, 552, 523, 532, 533, 542, 543,552, 553, 562, 563, 572, 573, 582, 583, 592, 593, 602, 603, 612, 613, 622, 623, 690,691, 700-737 and 739.

Embodiment 77. The polynucleotide of any one of Embodiments 73-76, comprising:(i) a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or that comprises or consists of, the nucleotide sequence set forth in SEQ ID NO. :407; and 129 WO 2021/226560 PCT/US2021/031442 (11) a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or that comprises or consists of, the nucleotide sequence set forth in SEQ ID NO. :408, 737, or 739.

Embodiment 78. A recombinant vector comprising the polynucleotide of any one of Embodiments 73-77.

Embodiment 79. A host cell comprising the polynucleotide of any one of Embodiments 77 and/or the vector of Embodiment78, wherein the polynucleotide is heterologous to the host cell.

Embodiment 80. A human B cell comprising the polynucleotide of any one of Embodiments 73-77, wherein polynucleotide is heterologous to the human B cell and/or wherein the human B cell is immortalized.

Embodiment 81. A composition comprising: (i) the antibody or antigen- binding fragment of any one of Embodiments 1-72; (ii) the polynucleotide of any one of Embodiments 73-77; (iii) the recombinant vector of Embodiment 78; (iv) the host cell of Embodiment 79; and/or (v)the human B cell of Embodiment 80, and a pharmaceutically acceptable excipient, carrier, or diluent.

Embodiment 82. The composition of Embodiment 81, comprising two or more antibodies or antigen-binding fragments of any of Embodiments 1-72.

Embodiment 83. The composition of Embodiment 82, comprising:(i) a first antibody or antigen-binding fragment, comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 32 and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 36; and 130 WO 2021/226560 PCT/US2021/031442 (11) a second antibody or antigen-binding fragment comprising, a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 1and a VL comprising of consisting of the amino acid sequence as set forth in SEQ ID NO: 143.

Embodiment 84. The composition of Embodiment 82, comprising:(i) a first antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 33-35, respectively, and the CDRL1, CDRL2, and CDRLcomprise or consist of the amino acid sequences set forth in SEQ ID NOs: 37-39, respectively; and(ii) a second antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 140-142, respectively, and the CDRL1, CDRL2, and CDRLcomprise or consist of the amino acid sequences set forth in SEQ ID NOs: 144-146, respectively.

Embodiment 85. The composition of Embodiment 82, comprising:(i) a first antibody or antigen-binding fragment, comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 139 or 342 and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 143 or 346; and(ii) a second antibody or antigen-binding fragment comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761, and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 403, 744, or 746. 131 WO 2021/226560 PCT/US2021/031442 Embodiment 86. The composition of Embodiment 82, comprising:(i) a first antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 140-142, respectively, or 343-345, respectively, and the CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 144-146, respectively; and(ii) a second antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively, and the CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively.

Embodiment 87. A composition comprising:(i) a first antibody or antigen-binding fragment, comprising(i)(a) a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 32, and(i)(b) a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 36; and(ii) a second antibody or antigen-binding fragment comprising(ii)(a) a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 139 and(ii)(b) a VL comprising of consisting of the amino acid sequence as set forth in SEQ ID NO: 143.

Embodiment 88. A composition comprising: 132 WO 2021/226560 PCT/US2021/031442 (1) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:400, 402, and 766, respectively, and(i)(b) a VL comprising the CDRL1, CDRL2, and CDRL3 amino acid sequences set forth in SEQ ID NOs.:404, 405, and 406, respectively; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:140, 141 or 344, and 142, respectively, and(ii)(b) a VL comprising CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.:144, 145, and 146, respectively.

Embodiment 89. A composition comprising:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the amino acid sequence set forth in SEQ ID NO.:399 and(i)(b) a VL comprising the amino acid sequence set forth in SEQ ID NOs.:403 or SEQ ID NO.:738; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the amino acid sequence set forth in SEQ ID NOs.:139 or 342, and(ii)(b) a VL comprising the amino acid sequence set forth in SEQ ID NO.:143. 133 WO 2021/226560 PCT/US2021/031442 Embodiment 90. The composition of any one of Embodiments 82-89, wherein the first antibody or antigen-binding fragment and the second antibody or antigen-binding fragment each comprise an IgGl Fc polypeptide comprising a M428L mutation and a N434S mutation.

Embodiment 91. The composition of any one of Embodiments 82-90, wherein the first antibody or antigen-binding fragment and the second antibody or antigen-binding fragment each comprise an IgGl Fc polypeptide comprising a G236A mutation, a A330L mutation, and a I332E mutation.

Embodiment 92. A composition comprising the polynucleotide of any one of Embodiments 73-77 encapsulated in a carrier molecule, wherein the carrier molecule optionally comprises a lipid, a lipid-derived delivery vehicle, such as a liposome, a solid lipid nanoparticle, an oily suspension, a submicron lipid emulsion, a lipid microbubble, an inverse lipid micelle, a cochlear liposome, a lipid microtubule, a lipid microcylinder, lipid nanoparticle (LNP), or a nanoscale platform.

Embodiment 93. A composition comprising:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS- C0V-2 surface glycoprotein and a first cell surface receptor selected from ACE2, DC- SIGN, L-SIGN, and SIGLEC-1; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS-CoV-2 surface glycoprotein and a second cell surface receptor selected from ACE2, DC-SIGN, L-SIGN, and SIGLEC-1,wherein the first cell surface receptor and the second cell surface receptor are different. 134 WO 2021/226560 PCT/US2021/031442 Embodiment 94. A method of treating a coronavirus infection, e.g. a SARS-CoV-2 infection, in a subject, the method comprising administering to the subject an effective amount of: (i) the antibody or antigen-binding fragment of any one of Embodiments 1-72; (ii) the polynucleotide of any one of Embodiments 73-77;(iii) the recombinant vector of Embodiment 78; (iv) the host cell of Embodiment 79; (v) the human B cell of Embodiment 80; and/or (vi) the composition of any one of Embodiments 81-93.

Embodiment 95. A method of treating a coronavirus infection, e.g. a SARS-CoV-2 infection, in a subject, the method comprising administering to the subject:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:400, 402, and 766, respectively, and(i)(b) a VL comprising the CDRL1, CDRL2, and CDRL3 amino acid sequences set forth in SEQ ID NOs.:404, 405, and 406, respectively; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:140, 141 or 344, and 142, respectively, and(ii)(b) a VL comprising CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.:144, 145, and 146, respectively. 135 WO 2021/226560 PCT/US2021/031442 Embodiment 96. A method of treating a coronavirus infection, e.g. aSARS-CoV-2 infection, in a subject, the method comprising administering to the subject:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the amino acid sequence set forth in SEQ ID NO.:399 and(i)(b) a VL comprising the amino acid sequence set forth in SEQ ID NOs.:403 or SEQ ID NO.:738; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the amino acid sequence set forth in SEQ ID NOs.:139 or 342, and(ii)(b) a VL comprising the amino acid sequence set forth in SEQ ID NO.:143.

Embodiment 97. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) VH and VL amino acid sequences according to SEQ ID NOs.:and 36 respectively; or(i)(b) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOS.:33-35 and 37-39, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) a VH amino acid sequence according to SEQ ID NO.: 139, and aVL amino acid sequence according to SEQ ID NO: 143; or(ii)(b) CDRH1, CDRH2, and CDRH3 amino acids according to SEQ ID NOs: 140-142, respectively, and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 144-146. 136 WO 2021/226560 PCT/US2021/031442 Embodiment 98. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) a VH amino acid sequence according to SEQ ID NO.: 139, and aVL amino acid sequence according to SEQ ID NO: 143; or(i)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 140-142, respectively; and CDRL1, CDRL2, and CDRLamino acid sequences according to SEQ ID NOs: 144-146, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) VH and VL amino acid sequences according to SEQ ID NOs. :and 36 respectively; or(ii)(b) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOS.:33-35 and 37-39, respectively.

Embodiment 99. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) a VH amino acid sequence according to SEQ ID NO.: 139 or 342 and a VL amino acid sequence according to SEQ ID NO: 143 or 346; or(i)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 140-142, respectively, or 343-345, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 144-146, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) a VH amino acid sequence according to SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761 and a VL amino acid sequence according to SEQ ID NO: 403, 744, or 746; or(ii)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively. 137 WO 2021/226560 PCT/US2021/031442 Embodiment 100. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) a VH amino acid sequence according to SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761 and a VL amino acid sequence according to SEQ ID NO: 403, 744, or 746; or(i)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ IDNOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) a VH amino acid sequence according to SEQ ID NO.: 139 or 342 and a VL amino acid sequence according to SEQ ID NO: 143 or 346; or(ii)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 140-142, respectively, or 343-345, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 144-146, respectively.

Embodiment 101. The method of any one of Embodiments 95-100, wherein the first antibody or antigen-binding fragment and the second antibody or antigen- binding fragment each comprise an IgGl Fc polypeptide comprising a M428L mutation and a N434S mutation.

Embodiment 102. The method of any one of Embodiments 95-101, wherein the first antibody or antigen-binding fragment and the second antibody or antigen- binding fragment each comprise an IgGl Fc polypeptide comprising a G236A mutation, a A330L mutation, and a I332E mutation.

Embodiment 103. A method of treating a coronavirus (e.g. SARS-CoV-2) infection in a subject, the method comprising administering to the subject: 138 WO 2021/226560 PCT/US2021/031442 (1) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS- C0V-2 surface glycoprotein and a first cell surface receptor selected from ACE2, DC- SIGN, L-SIGN, and SIGLEC-1; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS-CoV-2 surface glycoprotein and a second cell surface receptor selected from ACE2, DC-SIGN, L-SIGN, and SIGLEC-1,wherein the first cell surface receptor and the second cell surface receptor are different.

Embodiment 104. The antibody or antigen-binding fragment of any one of claims 1-72, the polynucleotide of any one of Embodiments 73-77, the recombinant vector of Embodiment 78, the host cell of Embodiment 79, the human B cell of Embodiment 80, and/or the composition of any one of Embodiments 81-93 for use in a method of treating a coronavirus (e.g. SARS-CoV-2) infection in a subject.

Embodiment 105. The antibody or antigen-binding fragment of any one of claims 1-72, the polynucleotide of any one of Embodiments 73-77, the recombinant vector of Embodimenf78, the host cell of Embodiment 79, the human B cell of Embodiment 80, and/or the composition of any one of Embodiments 81-93 for use in the preparation of a medicament for the treatment of a coronavirus (e.g. SARS-CoV-2) infection in a subject.

Embodiment 106. A method for in vitro diagnosis of a coronavirus (e.g. SARS-CoV-2) infection, the method comprising:(i) contacting a sample from a subject with an antibody or antigen-binding fragment of any one of Embodiments 1-72; and(ii) detecting a complex comprising an antigen and the antibody, or comprising an antigen and the antigen-binding fragment. 139 WO 2021/226560 PCT/US2021/031442 Embodiment 107. The method of Embodiment 106, wherein the sample comprises blood isolated from the subject. 140 WO 2021/226560 PCT/US2021/031442 Table 2. Sequences Sequence Description SEQ ID NO. Sequence SARS-CoV-2Wuhan seafood market pneumonia virus isolate Wuhan-Hu- genomic sequence (GenBank: MN908947.3;J anuary 23, 2020) 1 attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 61 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 1cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 181 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 2cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 301 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 3agactccgtg gaggaggtct tatcagaggc cgtcaacat cttaaagatg gcacttgtgg 421 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 4acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 541 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 6cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 661 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 7tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 781 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 8ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 901 atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 9tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1021 gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 10ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1141 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1201 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1261 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1321 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1381 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1441 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc1501 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1561 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 16aatactccaa aaagagaaag tcaacatcaa tattgttggt 141 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence gactttaaac ttaatgaaga 1681 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 17aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac1801 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1861 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 19tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1981 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 20taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg2101 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2161 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 22ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2281 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 23tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca2401 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2461 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 25aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2581 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 26aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2701 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 27agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2821 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 28ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc2941 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3001 tgagtttaaa ttggcttcac atatgtattg ttctttctac cctccagatg aggatgaaga 30agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3121 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 31agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3241 cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 33agagatggaa cttacaccag ttgttcagac tattgaagtg 142 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence aatagtttta gtggttattt 3361 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 34aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc3481 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3541 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 36acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3661 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 37tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa3781 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3841 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa3901 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3961 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 40cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4081 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4141 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat4201 gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4261 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc4321 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4381 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg4441 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4501 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 45gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4621 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 46agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc4741 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4801 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga4861 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4921 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4981 143 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5041 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 51acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5161 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5221 cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa5281 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5341 acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc5401 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5461 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 55taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5581 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 56agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5701 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 57gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt5821 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5881 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 59tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6001 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 60tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc6121 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6181 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 62gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6301 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 63cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6421 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 64aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6541 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 66attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 144 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence 6661 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6721 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 67ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc6841 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6901 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 69gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7021 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7081 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7141 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7201 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat7261 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7321 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 73acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7441 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 75ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag7561 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7621 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 76cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7741 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7801 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7861 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7921 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 79agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8041 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 81agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac8161 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8221 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 82ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8341 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8401 145 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc8461 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8521 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 85gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8641 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8701 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc8761 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8821 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 88gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8941 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9001 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata9061 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9121 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 91tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9241 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 93atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac9361 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9421 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 94tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9541 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9601 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9661 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9721 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 97tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa9841 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9901 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 99tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10021 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10081 146 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg10141 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10201 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 102ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10321 taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 103acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc10441 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10501 ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 105tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10621 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 106cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga10741 ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10801 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 108agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10921 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 109gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt11041 agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11101 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 111gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11221 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11281 tagtttgtct ggttttaagc taaaagactg tgttatgtat gcatcagctg tagtgttact 11341 aatccttatg acagcaagaa ctgtgtatga tgatggtgct aggagagtgt ggacacttat 11401 gaatgtcttg acactcgttt ataaagttta ttatggtaat gctttagatc aagccatttc 114catgtgggct cttataatct ctgttacttc taactactca ggtgtagtta caactgtcat 11521 gtttttggcc agaggtattg tttttatgtg tgttgagtat tgccctattt tcttcataac 11581 tggtaataca cttcagtgta taatgctagt ttattgtttc ttaggctatt tttgtacttg 11641 ttactttggc ctcttttgtt tactcaaccg ctactttaga ctgactcttg gtgtttatga 11701 ttacttagtt tctacacagg agtttagata tatgaattca cagggactac tcccacccaa 117gaatagcata gatgccttca aactcaacat taaattgttg 147 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ggtgttggtg gcaaaccttg 11821 tatcaaagta gccactgtac agtctaaaat gtcagatgta aagtgcacat cagtagtctt 118actctcagtt ttgcaacaac tcagagtaga atcatcatct aaattgtggg ctcaatgtgt11941 ccagttacac aatgacattc tcttagctaa agatactact gaagcctttg aaaaaatggt 12001 ttcactactt tctgttttgc tttccatgca gggtgctgta gacataaaca agctttgtga 120agaaatgctg gacaacaggg caaccttaca agctatagcc tcagagttta gttcccttcc 12121 atcatatgca gcttttgcta ctgctcaaga agcttatgag caggctgttg ctaatggtga 121ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat gtggctaaat ctgaatttga 12241 ccgtgatgca gccatgcaac gtaagttgga aaagatggct gatcaagcta tgacccaaat 12301 gtataaacag gctagatctg aggacaagag ggcaaaagtt actagtgcta tgcagacaat 12361 gcttttcact atgcttagaa agttggataa tgatgcactc aacaacatta tcaacaatgc 12421 aagagatggt tgtgttccct tgaacataat acctcttaca acagcagcca aactaatggt12481 tgtcatacca gactataaca catataaaaa tacgtgtgat ggtacaacat ttacttatgc 12541 atcagcattg tgggaaatcc aacaggttgt agatgcagat agtaaaattg ttcaacttag 126tgaaattagt atggacaatt cacctaattt agcatggcct cttattgtaa cagctttaag 12661 ggccaattct gctgtcaaat tacagaataa tgagcttagt cctgttgcac tacgacagat 12721 gtcttgtgct gccggtacta cacaaactgc ttgcactgat gacaatgcgt tagcttacta12781 caacacaaca aagggaggta ggtttgtact tgcactgtta tccgatttac aggatttgaa 12841 atgggctaga ttccctaaga gtgatggaac tggtactatc tatacagaac tggaaccacc 129ttgtaggttt gttacagaca cacctaaagg tcctaaagtg aagtatttat actttattaa 12961 aggattaaac aacctaaata gaggtatggt acttggtagt ttagctgcca cagtacgtct 130acaagctggt aatgcaacag aagtgcctgc caattcaact gtattatctt tctgtgcttt13081 tgctgtagat gctgctaaag cttacaaaga ttatctagct agtgggggac aaccaatcac 13141 taattgtgtt aagatgttgt gtacacacac tggtactggt caggcaataa cagttacacc 132ggaagccaat atggatcaag aatcctttgg tggtgcatcg tgttgtctgt actgccgttg 13261 ccacatagat catccaaatc ctaaaggatt ttgtgactta aaaggtaagt atgtacaaat 133acctacaact tgtgctaatg accctgtggg ttttacactt aaaaacacag tctgtaccgt13381 ctgcggtatg tggaaaggtt atggctgtag ttgtgatcaa ctccgcgaac ccatgcttca 13441 gtcagctgat gcacaatcgt 148 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca 135ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgacat ctacaatgat 13561 aaagtagctg gttttgctaa attcctaaaa actaattgtt gtcgcttcca agaaaaggac 13621 gaagatgaca atttaattga ttcttacttt gtagttaaga gacacacttt ctctaactac 13681 caacatgaag aaacaattta taatttactt aaggattgtc cagctgttgc taaacatgac 137ttctttaagt ttagaataga cggtgacatg gtaccacata tatcacgtca acgtcttact 13801 aaatacacaa tggcagacct cgtctatgct ttaaggcatt ttgatgaagg taattgtgac 138acattaaaag aaatacttgt cacatacaat tgttgtgatg atgattattt caataaaaag13921 gactggtatg attttgtaga aaacccagat atattacgcg tatacgccaa cttaggtgaa 13981 cgtgtacgcc aagctttgtt aaaaacagta caattctgtg atgccatgcg aaatgctggt 140attgttggtg tactgacatt agataatcaa gatctcaatg gtaactggta tgatttcggt 14101 gatttcatac aaaccacgcc aggtagtgga gttcctgttg tagattctta ttattcattg 141ttaatgccta tattaacctt gaccagggct ttaactgcag agtcacatgt tgacactgac14221 ttaacaaagc cttacattaa gtgggatttg ttaaaatatg acttcacgga agagaggtta 14281 aaactctttg accgttattt taaatattgg gatcagacat accacccaaa ttgtgttaac 143tgtttggatg acagatgcat tctgcattgt gcaaacttta atgttttatt ctctacagtg 14401 ttcccaccta caagttttgg accactagtg agaaaaatat ttgttgatgg tgttccattt 14461 gtagtttcaa ctggatacca cttcagagag ctaggtgttg tacataatca ggatgtaaac14521 ttacatagct ctagacttag ttttaaggaa ttacttgtgt atgctgctga ccctgctatg 14581 cacgctgctt ctggtaatct attactagat aaacgcacta cgtgcttttc agtagctgca 146cttactaaca atgttgcttt tcaaactgtc aaacccggta attttaacaa agacttctat 14701 gactttgctg tgtctaaggg tttctttaag gaaggaagtt ctgttgaatt aaaacacttc 14761 ttctttgctc aggatggtaa tgctgctatc agcgattatg actactatcg ttataatcta14821 ccaacaatgt gtgatatcag acaactacta tttgtagttg aagttgttga taagtacttt 14881 gattgttacg atggtggctg tattaatgct aaccaagtca tcgtcaacaa cctagacaaa 149tcagctggtt ttccatttaa taaatggggt aaggctagac tttattatga ttcaatgagt 15001 tatgaggatc aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact 15061 caaatgaatc ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc 15121 tctatctgta gtactatgac caatagacag 149 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence tttcatcaaa aattattgaa atcaatagcc 15181 gccactagag gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac 15241 atgttaaaaa ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct 15301 aaatgtgata gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc15361 aaacatacaa cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct 15421 caagtattga gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc 154tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc 15541 acggccaatg ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc 15601 cgcaatttac aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac15661 tttgtgaatg agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac 15721 gatgctgttg tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag 157aactttaagt cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg 15841 actgagactg accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt 15901 aaacagggtg atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc15961 ggctgttttg tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg 16021 tctttagcta tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc 160tttcatttgt acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta 16141 gacatgtatt ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt 162tatgaggcta tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc16261 aattcacaga cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa 16321 tgctgttacg accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat 163gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg 16441 agctattatt gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa 16501 gtttttggtt tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca 16561 attgcaacat gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa 16621 agactcaagc tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct 166tatggtattg ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa 16741 gttggtaaac ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact 168aaaaacagta aagtacaaat aggagagtac acctttgaaa 150 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence aaggtgacta tggtgatgct 16861 gttgtttacc gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca 169tcacatacag taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga16981 attactggct tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat 17041 tatcaaaagg ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag17101 agtcattttg ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct 17161 tgctctcatg ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat 172aaatgtagta gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg 17281 aattcaacat tagaacagta tgtcttttgt actgtaaatg cattgcctga gacgacagca 173gatatagttg tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat17401 gccagattac gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca 17461 cgcacattgc taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt 175atgaaaacta taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt 17581 gttgacactg tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca 176gctcaatgct ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt17701 aacaggccac aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa 17761 gctgtcttta tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta17821 ccaactcaaa ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa 17881 accactgaaa cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca 179aaagtaggca tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca 18001 agtcttgaaa ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc 180tttaaagatt gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc 18121 agtgttgaca ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag 181gacatgacct atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat18241 ggttacccta acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt 18301 ggcttcgatg tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta 183cagctaggtt tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca 18421 cctaataata cagatttttc cagagttagt gctaaaccac cgcctggaga tcaatttaaa 18481 cacctcatac cacttatgta caaaggactt ccttggaatg tagtgcgtat 151 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence aaagattgta18541 caaatgttaa gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca 18601 catggctttg agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt18661 tgtctatgtg atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg 18721 catcattcta ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg 187ggttttacag gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca 18841 catgtagcta gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt 189aagcgtgttg actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg18961 gcttgtagaa aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca 19021 gttcttcacg acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa19081 tggaagttct atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc 19141 tattcttatg ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc 192aatgtcgata gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct 19261 aaccttaact tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac 19321 acaccagctt ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac 19381 tctgacagtc catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca 19441 ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat 195gctaatgagt acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc 19561 ttgtgggttt acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag 196agtttagaaa atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt19681 gaagtaccag tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta 19741 gaattgtttg aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag 198cgcaacatta aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct 19861 gctaatactg tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt 199gtttgttcta tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accactcact 19981 gtcttttttg atggtagagt tgatggtcaa gtagacttat ttagaaatgc ccgtaatggt 200gttcttatta cagaaggtag tgttaaaggt ttacaaccat ctgtaggtcc caaacaagct 20101 agtcttaatg gagtcacatt aattggagaa gccgtaaaaa cacagttcaa ttattataag 201aaagttgatg gtgttgtcca acaattacct gaaacttact 152 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ttactcagag tagaaattta20221 caagaattta aacccaggag tcaaatggaa attgatttct tagaattagc tatggatgaa 20281 ttcattgaac ggtataaatt agaaggctat gccttcgaac atatcgttta tggagatttt 203agtcatagtc agttaggtgg tttacatcta ctgattggac tagctaaacg ttttaaggaa 20401 tcaccttttg aattagaaga ttttattcct atggacagta cagttaaaaa ctatttcata 204acagatgcgc aaacaggttc atctaagtgt gtgtgttctg ttattgattt attacttgat20521 gattttgttg aaataataaa atcccaagat ttatctgtag tttctaaggt tgtcaaagtg 20581 actattgact atacagaaat ttcatttatg ctttggtgta aagatggcca tgtagaaaca 206ttttacccaa aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt 20701 tacaaaatgc aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca 207acattaccta aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta20821 aacacattaa cattagctgt accctataat atgagagtta tacattttgg tgctggttct 20881 gataaaggag ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg 209cttgtcgatt cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat 21001 tgtgcaactg tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct 21061 aagactaaaa atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt 21121 gggtttatac aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat 21181 tcttggaatg ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt21241 actaatgtga atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa 21301 ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca 213aatccaattc agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta 21421 aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt 21481 cttagtaaag gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt 21541 cttgttaaca actaaacgaa caatgtttgt ttttcttgttttattgccac tagtctctag21601 tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac 21661 acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga 217cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac 21781 caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc 21841 ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt 153 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence tagattcgaa21901 gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt 21961 tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat 220ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca 22081 gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt 221gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt22201 gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat 22261 taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga 223ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag 22381 gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact 22441 tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta22501 tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac 22561 aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg 226gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc 22681 attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac 227taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg 22801 gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt 228tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta22921 tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta 22981 tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca 230atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact 23101 ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt 231ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac23221 tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac 23281 tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg 233tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca 23401 ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg 234gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc 154 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence 23521 tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag 23581 ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat 236tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc 23701 catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa 23761 gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt 23821 gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga 23881 acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc 239aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag 24001 caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt 24061 catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca24121 aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata 24181 cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc 242attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca 24301 gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa 24361 aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa24421 ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat 24481 ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat 245tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat 24601 tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt 246acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc24721 tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa 24781 gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg24841 tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca 24901 aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt 249caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga 25021 taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa 250tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt25141 aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc 25201 atggtacatt tggctaggtt 155 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ttatagctgg cttgattgcc atagtaatgg tgacaattat 252gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg 25321 ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac 25381 ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag 25441 caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg 25501 atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt 255cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt 25621 gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc 256gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag 25741 agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa 25801 aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat 25861 tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca 25921 agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga 259gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca 26041 actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt 26101 gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acggttcatc cggagttgtt26161 aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa 26221 gcacaagctg atgagtacga acttatgtac tcattcgttt cggaagagac aggtacgtta 262atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc 26341 atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta 26401 aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat 26461 cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag 26521 ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat 265ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg 26641 ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag 26701 taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa 26761 ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt 26821 tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc 268tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa 26941 tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg 270acatcaagga cctgcctaaa gaaatcactg ttgctacatc 156 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence acgaacgctt tcttattaca27061 aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca 27121 ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc 271ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag 27241 atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata 27301 aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat 27361 gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg 27421 ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta 274cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta 27541 gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac 276ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga27661 caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt 27721 ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact 277tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt 27841 ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat 27901 ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac27961 agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt 28021 ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg 280atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct 28141 gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt 282cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa28261 cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac 28321 gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg 28381 atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct 28441 cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac 285caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg 28561 tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg 286gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga 28681 gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc 28741 157 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag 28801 cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa 28861 ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca atggcggtga 28921 tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg 28981 taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 29041 gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 29101 acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac 29161 tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg 29221 aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc 29281 catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca29341 tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 29401 tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 29461 tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 29521 aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc 29581 ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc29641 acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta 29701 gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt29761 acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat 29821 tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaatgac aaaaaaaaaa 298aaaaaaaaaa aaaaaaaaaa aaaSARS-CoV-2Wuhan seafood market pneumonia virus isolate Wuhan-Hu- genomic sequence (GenBank:MN908947.3;J anuary 23, 2020) - amino acid translation MESLVPGFNEKTHVQLSLPVLQVRDVLVRGF GDSVEEVLSEARQHLKDGTCGLVEVEKGVLP QLEQPYVFIKRSDARTAPHGHVMVELVAELE GIQYGRSGETLGVLVPHVGEIPVAYRKVLLR KNGNKGAGGHSYGADLKSFDLGDELGTDPY EDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNF CGPDGYPLECIKDLLARAGKASCTLSEQLDFI DTKRGVYCCREHEHEIAWYTERSEKSYELQT PFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPR VEKKKLDGFMGRIRSVYPVASPNECNQMCLS TLMKCDHCGETSWQTGDFVKATCEFCGTEN LTKEGATTCGYLPQNAVVKIYCPACHNSEVG 158 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence PEHSLAEYHNESGLKTILRKGGRTIAFGGCVF SYVGCHNKCAYWVPRASANIGCNHTGVVGE GSEGLNDNLLEILQKEKVNINTVGDFKLNEEIAIILASF S AST SAFVETVKGLDYKAFKQIVESCGNFKVTKGK AKKGAWNIGEQKSILSPLYAFASEAARVVRSI FSRTLETAQNSVRVLQKAAITILDGISQYSLRL IDAMMFTSDLATNNLVVMAYITGGVVQLTSQ WLTNIFGTVYEKLKPVLDWLEEKFKEGVEFL RDGWEIVKFISTCACEIVGGQIVTCAKEIKESV QTFFKLVNKFLALCADSIIIGGAKLKALNLGE TFVTHSKGLYRKCVKSREETGLLMPLKAPKEI IFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSE AVEAPLVGTPVCINGLMLLEIKDTEKYCALAP NMMVTNNTFTLKGGAPTKVTFGDDTVIEVQ GYKS VNITFELDERIDKVLNEKC S AYTVELGT EVNEFACVVADAVIKTLQPVSELLTPLGIDLD EWSMATYYLFDESGEFKLASHMYCSFYPPDE DEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLE FGATSAALQPEEEQEEDWLDDDSQQTVGQQ DGSEDNQTTTIQTIVEVQPQLEMELTPVVQTI EVNSF SGYLKLTDNVYIKNADIVEEAKK VKP TVVVNAANVYLKHGGGVAGALNKATNNAM QVESDDYIATNGPLKVGGSCVLSGHNLAKHC LHVVGPNVNKGEDIQLLKSAYENFNQHEVLL APLLSAGIFGADPIHSLRVCVDTVRTNVYLAV FDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKE EVKPFITESKPSVEQRKQDDKKIKACVEEVTT TLEETKFLTENLLLYIDINGNLHPDSATLVSDI DITFLKKDAPYIVGDVVQEGVLTAVVIPTKKA GGTTEMLAKALRKVPTDNYITTYPGQGLNGY TVEEAKTVLKKCKSAFYILPSIISNEKQEILGT VSWNLREMLAHAEETRKLMPVCVETKAIVST IQRKYKGIKIQEGVVDYGARFYFYTSKTTVAS LINTLNDLNETLVTMPLGYVTHGLNLEEAAR YMRSLKVPATVSVSSPDAVTAYNGYLTSSSK TPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLL SLREVRTIKVFTTVDNINLHTQVVDMSMTYG QQFGPTYLDGADVTKIKPHNSHEGKTFYVLP NDDTLRVEAFEYYHTTDPSFLGRYMSALNHT I 159 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence YEQFKKGVQIPCTCGKQATKYLVQQESPFVM MSAPPAQYELKHGTFTCASEYTGNYQCGHY KHITSKETLYCIDGALLTKSSEYKGPITDVFYK ENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYK KDNSYFTEQPIDLVPNQPYPNASFDNFKFVCD NIKFADDLNQLTGYKKPASRELKVTFFPDLN GDVVAIDYKHYTPSFKKGAKLLHKPIVWHVN NATNKATYKPNTWCIRCLWSTKPVETSNSFD VLKSEDAQGMDNLACEDLKPVSEEVVENPTI QKDVLECNVKTTEVVGDIILKPANNSLKITEE VGHTDLMAAYVDNS SLTIKKPNELSRVLGLK TLATHGLAAVNSVPWDTIANYAKPFLNKVVS TTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFT RSTNSRIKASMPTTIAKNTVKSVGKFCLEASF NYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAA LGVLMSNLGMPSYCTGYREGYLNSTNVTIAT YCTGSIPCSVCLSGLDSLDTYPSLETIQITISSF KWDLTAFGLVAEWFLAYILFTRFFYVLGLAA IMQLFFSYFAVHFISNSWLMWLIINLVQMAPI SAMVRMYIFFASFYYVWKSYVHVVDGCNSS TCMMCYKRNRATRVECTTIVNGVRRSFYVY ANGGKGFCKLHNWNCVNCDTFCAGSTFISDE VARDLSLQFKRPINPTDQS S YIVD S VT VKNGSI HLYFDKAGQKTYERHSLSHFVNLDNLRANNT KGSLPINVIVFDGKSKCEESSAKSASVYYSQL MCQPILLLDQALVSDVGDSAEVAVKMFDAY VNTF S STFNVPMEKLKTL VAT AEAELAKNVS LDNVLSTFISAARQGFVDSDVETKDVVECLK LSHQSDIEVTGDSCNNYMLTYNKVENMTPRD LGACIDCSARHINAQVAKSHNIALIWNVKDF MSLSEQLRKQIRSAAKKNNLPFKLTCATTRQ VVNVVTTKIALKGGKIVNNWLKQLIKVTLVF LFVAAIFYLITPVHVMSKHTDF S SEIIGYKAID GGVTRDIASTDTCFANKHADFDTWFSQRGGS YTNDKACPLIAAVITREVGFVVPGLPGTILRTT NGDFLHFLPRVFSAVGNICYTPSKLIEYTDFAT SACVLAAECTIFKDASGKPVPYCYDTNVLEG SVAYESLRPDTRYVLMDGSIIQFPNTYLEGSV RVVTTFDSEYCRHGTCERSEAGVCVSTSGRW VLNNDYYRSLPGVFCGVDAVNLLTNMFTPLI QPIGALDISASIVAGGIVAIVVTCLAYYFMRFR RAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSF LPGVYSVIYLYLTFYLTNDVSFLAHIQWMVM FTPLVPFWITIAYIICISTKHF YWFF SNYLKRRV VFNGVSF STFEEAALCTFLLNKEMYLKLRSD 160 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence VLLPLTQYNRYLALYNKYKYFSGAMDTTSY REAACCHLAKALNDFSNSGSDVLYQPPQTSIT SAVLQSGFRKMAFPSGKVEGCMVQVTCGTT TLNGLWLDDVVYCPRHVICTSEDMLNPNYED LLIRKSNHNFLVQAGNVQLRVIGHSMQNCVL KLKVDT ANPKTPKYKF VRIQPGQTF S VL AC Y NGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGF NIDYDCVSFCYMHHMELPTGVHAGTDLEGN FYGPFVDRQTAQAAGTDTTITVNVLAWLYA AVINGDRWFLNRFTTTLNDFNLVAMKYNYEP LTQDHVDILGPLSAQTGIAVLDMCASLKELLQ NGMNGRTILGSALLEDEFTPFDVVRQCSGVTF QS A vkrtikgthhwllltiltsllvlvqstq WSLFFFLYENAFLPFAMGIIAMSAFAMMFVK HKHAFLCLFLLPSLATVAYFNMVYMPASWV MRIMTWLDMVDTSLSGFKLKDCVMYASAVV LLILMTARTVYDDGARRVWTLMNVLTLVYK VYYGNALDQAISMWALIISVTSNYSGVVTTV MFLARGIVFMCVEYCPIFFITGNTLQCIMLVY CFLGYFCTCYFGLFCLLNRYFRLTLGVYDYL VSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLG VGGKPCIKVATVQSKMSDVKCTSVVLLSVLQ QLRVESSSKLWAQCVQLHNDILLAKDTTEAF EKMVSLLSVLLSMQGAVDINKLCEEMLDNR ATLQAIASEFSSLPSYAAFATAQEAYEQAVAN GDSEVVLKKLKKSLNVAKSEFDRDAAMQRK LEKMADQAMTQMYKQARSEDKRAKVTSAM QTMLFTMLRKLDNDALNNIINNARDGCVPLN IIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYA SALWEIQQVVDADSKIVQLSEISMDNSPNLA WPLIVTALRANSAVKLQNNELSPVALRQMSC AAGTTQTACTDDNALAYYNTTKGGRFVLAL LSDLQDLKWARFPKSDGTGTIYTELEPPCRFV TDTPKGPKVKYLYFIKGLNNLNRGMVLGSLA ATVRLQAGNATEVPANSTVLSFCAFAVDAAK AYKDYLASGGQPITNCVKMLCTHTGTGQAIT VTPEANMDQESFGGASCCLYCRCHIDHPNPK GFCDLKGKYVQIPTTCANDPVGFTLKNTVCT VCGMWKGYGCSCDQLREPMLQSADAQSFLN RVCGVSAARLTPCGTGTSTDVVYRAFDIYND KVAGFAKFLKTNCCRFQEKDEDDNLIDSYFV VKRHTF SNYQHEETIYNLLKDCP AVAKHDFF KFRIDGDMVPHISRQRLTKYTMADLVYALRH FDEGNCDTLKEILVTYNCCDDDYFNKKDWY DFVENPDILRVYANLGERVRQALLKTVQFCD 161 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AMRNAGIVGVLTLDNQDLNGNWYDFGDFIQ TTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYI KWDLLKYDFTEERLKLFDRYFKYWDQTYHP NCVNCLDDRCILHCANFNVLFSTVFPPTSFGP LVRKIFVDGVPFVVSTGYHFRELGVVHNQDV NLHS SRLSFKELL VYAADPAMHAASGNLLLD KRTTCF S VAALTNNVAFQTVKPGNFNKDF YD FAVSKGFFKEGSSVELKHFFFAQDGNAAISDY DYYRYNLPTMCDIRQLLFVVEVVDKYFDCY DGGCINANQVIVNNLDKSAGFPFNKWGKARL YYD SMS YEDQDALF AYTKRNVIPTITQMNLK YAIS AKNRART VAGVSIC STMTNRQFHQKLL KSIAATRGATVVIGTSKFYGGWHNMLKTVYS DVENPHLMGWDYPKCDRAMPNMLRIMASL VLARKHTTCCSLSHRFYRLANECAQVLSEMV MCGGSLYVKPGGTSSGDATTAYANSVFNICQ AVTANVNALLSTDGNKIADKYVRNLQHRLY ECLYRNRDVDTDFVNEFYAYLRKHFSMMILS DDAVVCFNSTYASQGLVASIKNFKSVLYYQN NVFMSEAKCWTETDLTKGPHEFC SQHTML V KQGDDYVYLPYPDPSRILGAGCFVDDIVKTD GTLMIERFVSLAIDAYPLTKHPNQEYADVFHL YLQYIRKLHDELTGHMLDMYSVMLTNDNTS RYWEPEFYEAMYTPHTVLQAVGACVLCNSQ TSLRCGACIRRPFLCCKCCYDHVISTSHKLVL SVNPYVCNAPGCDVTDVTQLYLGGMSYYCK SHKPPISFPLCANGQVFGLYKNTCVGSDNVTD FNAIATCDWTNAGDYILANTCTERLKLFAAE TLKATEETFKLSYGIATVREVLSDRELHLSWE VGKPRPPLNRNYVFTGYRVTKNSKVQIGEYT FEKGDYGDAVVYRGTTTYKLNVGDYFVLTS HTVMPLSAPTLVPQEHYVRITGLYPTLNISDE F S SNVANYQKVGMQKYSTLQGPPGTGKSHF AIGLALYYPSARIVYTACSHAAVDALCEKAL KYLPIDKC SRIIP ARARVECFDKFK VNSTLEQ Y VFCTVNALPETTADIVVFDEISMATNYDLSVV NARLRAKHYVYIGDPAQLPAPRTLLTKGTLE PEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVD TVSALVYDNKLKAHKDKSAQCFKMFYKGVI THD VS S AINRPQIG V VREF LTRNP AWRK AVFI SPYNSQNAVASKILGLPTQTVDSSQGSEYDYV IFTQTTETAHSCNVNRFNVAITRAKVGILCIMS DRDLYDKLQFTSLEIPRRNVATLQAENVTGLF KDCSKVITGLHPTQAPTHLSVDTKFKTEGLCV 162 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence DIPGIPKDMTYRRLISMMGFKMNYQVNGYPN MFITREEAIRHVRAWIGFDVEGCHATREAVG TNLPLQLGFSTGVNLVAVPTGYVDTPNNTDF SRVSAKPPPGDQFKHLIPLMYKGLPWNVVRI KIVQMLSDTLKNLSDRVVFVLWAHGFELTSM KYFVKIGPERTCCLCDRRATCFSTASDTYAC WHHSIGFDYVYNPFMIDVQQWGFTGNLQSN HDLYCQVHGNAHVASCDAIMTRCLAVHECF VI mfvflvllpl vssqcvnltt rtqlppaytn sftrgvyypd kvfrssvlhs tqdlflpffs 61 nvtwfhaihv sgtngtkrfd npvlpfndgv yfasteksni irgwifgttl dsktqslliv 1nnatnvvikv cefqfcndpf Igvyyhknnk swmesefrvy ssannctfey vsqpflmdle181 gkqgnfknlr efvfknidgy fkiyskhtpi nlvrdlpqgf saleplvdlp iginitrfqt 2llalhrsylt pgdsssgwta gaaayyvgyl qprtfllkyn 163 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GenBank: QHD43416.1; January 23, 2020 engtitdavd caldplsetk 301 ctlksftvek giyqtsnfrv qptesivrfp nitnlcpfge vfnatrfasv yawnrkrisn 3cvadysvlyn sasfstfkcy gvsptklndl cftnvyadsf virgdevrqi apgqtgkiad 421 ynyklpddft gcviawnsnn Idskvggnyn ylyrlfrksn Ikpferdist eiyqagstpc 4ngvegfncyf plqsygfqpt ngvgyqpyrv vvlsfellha patvcgpkks tnlvknkcvn 541 fnfngltgtg vltesnkkfl pfqqfgrdia dttdavrdpq tleilditpc sfggvsvitp 6gtntsnqvav lyqdvnctev pvaihadqlt ptwrvystgs nvfqtragcl igaehvnnsy 661 ecdipigagi casyqtqtns prrarsvasq siiaytmslg aensvaysnn siaiptnfti 7svtteilpvs mtktsvdctm yicgdstecs nlllqygsfc tqlnraltgi aveqdkntqe 781 vfaqvkqiyk tppikdfggf nfsqilpdps kpskrsfied llfnkvtlad agfikqygdc 8Igdiaardli caqkfngltv Ipplltdemi aqytsallag titsgwtfga gaalqipfam 901 qmayrfngig vtqnvlyenq klianqfnsa igkiqdslss tasalgklqd vvnqnaqaln 9tlvkqlssnf gaissvlndi Isrldkveae vqidrlitgr Iqslqtyvtq qliraaeira1021 sanlaatkms ecvlgqskrv dfcgkgyhlm sfpqsaphgv vflhvtyvpa qeknfttapa 1081 ichdgkahfp regvfvsngt hwfvtqrnfy epqiittdnt fvsgncdvvi givnntvydp 1141 Iqpeldsfke eldkyfknht spdvdlgdis ginasvvniq keidrlneva knlneslidl 1201 qelgkyeqyi kwpwyiwlgf iagliaivmv timlccmtsc csclkgccsc gscckfdedd 1261 sepvlkgvkl hytsurface glycoprotein RBD [SARS- C0V-2 Wuhan seafood market pneumonia virus];GenBank: QHD43416.1;January 23, 2020 nitnlcpfgevfnatrfasvyawnrkrisncvadysvlynsasfstfkc ygvsptklndlcftnvyadsfvirgdevrqiapgqtgkiadynyklpd dftgcviawnsnnldskvggnynylyrlfrksnlkpferdisteiyqa gstpcngvegfncyfplqsygfqptngvgyqpyrvvvlsfellhapa tvcgpkkstnlvknkcvnfnfngltgtg Receptor Binding Motif (RBM) in surface glycoprotein RBD [SARS- C0V-2 Wuhan 5Nsnnldskvggnynylyrlfrksnlkpferdisteiyqagstpcngve gfncyfplqsygfqptngvgyqpy 164 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence seafood market pneumonia virus];GenBank: QHD43416.1; January 23, 2020 SARS-CoV-2CH1-CH3 LSGlml7IgHGl*01 (aa) ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDY FPEPVTVSWNSGALTSGVHTFPAVLQSSGLYS LSSVVTVPSSSLGTQTYICNVNHKPSNTKVDK KVEPKSCDKTHTCPPCPAPELLGGPSVFLFPP KPKDTLMISRTPEVTCVVVDVSHEDPEVKFN WYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTIS KAKGQPREPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVLHE ALHSHYTQKSLSLSPGK SARS-CoV-2CH1-CH3 LS, ALEGlm17IgHGl*01 (aa) ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDY FPEPVTVSWNSGALTSGVHTFPAVLQSSGLYS LSSVVTVPSSSLGTQTYICNVNHKPSNTKVDK KVEPKSCDKTHTCPPCPAPELLAGPSVFLFPP KPKDTLMISRTPEVTCVVVDVSHEDPEVKFN WYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPLPEEKTIS KAKGQPREPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVLHE ALHSHYTQKSLSLSPGK SARS-CoV-2CLIgLC*(aa) GQPKAAPSVTLFPPSSEELQANKATLVCLISDF YPGAVTVAWKADSSPVKAGVETTTPSKQSN NKYAASSYLSLTPEQWKSHRSYSCQVTHEGS TVEKTVAPTECS SARS-CoV-2CL (CK) klmIgKC*01 (aa) RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNF YPREAKVQWKVDNALQSGNSQESVTEQDSK DSTYSLSSTLTLSKADYEKHKVYACEVTHQG LSSPVTKSFNRGEC Linker (aa) 10GSTSGSGKPGSGEGSTKG Linker (aa) 11GSGKPGSGEG 165 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence Linker (aa) 12GKPGSGEG Linker (aa) 13SGKPGSGE Linker (aa) 14BPXXXZ, wherein each X is independently a glycine (G) or serine (S), B is a positively charged amino acid and Z is glycine (G) or a negatively charged amino acidLinker (aa) 15(GxS)y, wherein x is 1-10 and y is 1-10 Linker (aa) 16GGGGSGGGGSGGGGS Linker (aa) 17GGGGSGGGGSGGGGSGGGGSGGGGS GGGGSGGGGSGGGGSGGGGSGGGGSLinker (aa) 18GSTSGGGSGGGSGGGGSS Linker (aa) 19EGKSSGSGSESKVD Linker (aa) 20KESGSVSSEQLAQFRSLD Linker (aa) 21GGGGS SARS-CoV-2S2X16-VmAh VH (aa) EVQLVQSGAEVKKPGASVKVSCKASGYIFTD HYMHWVRQAPGQGLEWMGWINPNSGGTN YAQKFQGRVTMTRDTSINTAYMELSRLRSDD TAVHYCARDRSRFRFFSPDFDYWGQGTLVT vss SARS-CoV-S2X16-VmAhCDRH(aa) GYIFTDHY SARS-CoV-S2X16-VmAhCDRH(aa) INPNSGGT SARS-CoV-S2X16-VmAhCDRH(aa) ARDRSRFRFFSPDFDY SARS-CoV-S2X16-VmAh VL (aa) EIVLTQSPLSLPVTPGEPASISCRSSQSLLHSN GYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTPPTFGQGTKVEIK SARS-CoV-2 QSLLHSNGYNY 166 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2X16-VmAhCDRL(aa)SARS-CoV-S2X16-VmAh CDRL(aa) LGS SARS-CoV-S2X16-VmAh CDRL(aa) MQALQTPPT SARS-CoV-2S2X16-VmAh VH (nt) GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACATCTTCACC GACCACTATATGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTGGCACAAACT ATGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACACGTCCATCAACACAGCCT ACATGGAGCTGAGCAGGCTGAGATCTGACG ACACGGCCGTGCATTACTGTGCGAGAGAT CGGTCACGATTTCGATTTTTCTCCCCCGA CTTTGACTACTGGGGCCAGGGAACCCTGG TCACCGTCTCCTCAG SARS-CoV-S2X16-VmAh VL (nt) GAAATTGTGCTGACTCAGTCTCCACTCTCCC TGCCCGTCACCCCTGGAGAGCCGGCCTCCA TCTCCTGCAGGTCTAGTCAGAGCCTCCTGC ATAGTAATGGATACAACTATTTGGATTGG TACCTGCAGAAGCCAGGGCAGTCTCCACAG CTCCTGATCTATTTGGGTTCTAATCGGGCC TCCGGGGTCCCTGACAGGTTCAGTGGCAGT GGCTCAGGCACAGATTTCACACTGAAAATC AGCAGAGTGGAGGCTGAGGATGTTGGGGTT TATTACTGCATGCAAGCTCTACAAACTCC TCCAACGTTCGGCCAAGGGACCAAGGTGG AAATCAAAC SARS-CoV-2S2X28-VmAh VH (aa) EVQLVESGGGVVQPGRSLRLSCAASGFTFST YAMHWVRQAPGKGLEWVAVILSDGSNKYY ADSVKGRFTISRDNSKNTLYLQMNSLRAEDT AVYYCARDRSPLVGFGDNYGMDVWGQGTT VTVSS 167 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence SARS-CoV-S2X28-VmAhCDRH(aa)GFTFSTYA SARS-CoV-S2X28-VmAhCDRH(aa)ILSDGSNK SARS-CoV-S2X28-VmAhCDRH(aa)ARDRSPLVGFGDNYGMDV SARS-CoV-2S2X28-VmAh VL (aa) SYELTQPPSVSVSPGQTARITCSGDALPKKYA YWYQQKSGQAPVLVTYEDSKRPSGIPERFSGS SSGTMATLTISGAQVEDEADYYCSSTDSSGN QGVFGGGTKLTVL SARS-CoV-S2X28-VmAhCDRL(aa)ALPKKY SARS-CoV-S2X28-VmAh CDRL(aa)EDS SARS-CoV-S2X28-VmAh CDRL(aa) SSTDSSGNQGV SARS-CoV-2S2X28-VmAh VH (nt) GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CGTGGTCCAGCCTGGGAGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGATTCACCTTCAG TACCTATGCTATGCACTGGGTCCGCCAGGC TCCAGGCAAGGGGCTAGAGTGGGTGGCAGT TATATTATCTGATGGAAGTAATAAATATT ACGCAGACTCTGTGAAGGGCCGATTCACCA TCTCCAGAGACAATTCCAAGAACACGCTGT ATCTGCAAATGAACAGCCTGAGAGCTGAGG ACACGGCTGTGTATTACTGTGCGAGAGATC GAAGTCCCCTCGTGGGATTCGGGGACAA CTATGGTATGGACGTCTGGGGCCAAGGGA 168 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CCACGGTCACCGTCTCCTCA SARS-CoV-S2X28-VmAh VL (nt) TCCTATGAGCTGACACAGCCACCCTCGGTG TCAGTGTCCCCAGGACAAACGGCCAGGATC ACCTGCTCTGGAGATGCATTGCCAAAAAA ATATGCTTATTGGTACCAGCAGAAGTCAGG CCAGGCCCCTGTGCTGGTCATCTATGAGGA CAGCAAACGACCCTCCGGGATCCCTGAGA GATTCTCTGGCTCCAGCTCAGGGACAATGG CCACCTTGACTATCAGTGGGGCCCAGGTGG AGGATGAAGCTGACTACTACTGTTCCTCAA CAGACAGCAGTGGTAATCAAGGGGTATT CGGCGGAGGGACCAAGCTGACCGTCCTAG SARS-CoV-2S2X30-VmAh VH (aa) EVQLVQSGAEVKKPGSSVKVSCKASGGTFGS YTISWVRQAPGQGLEWMGRIIPILSIPNYAQK FQGRVTFTADKSTSTAYMELSSLRSEDTAVY YCARGGGGTHAVPHYYFDSWGQGTLVTVS S SARS-CoV-S2X30-VmAhCDRH(aa) GGTFGSYT SARS-CoV-S2X30-VmAhCDRH(aa) IIPILSIP SARS-CoV-S2X30-VmAhCDRH(aa) ARGGGGTHAVPHYYFDS SARS-CoV-2S2X30-VmAh VL (aa) EIVMMQSPATLSVSPGERATLSCRASQSVSSN L AW YQHKPGQ APRLLIYGASTRATGIP ARF S GSGSGTEFTLTISSMQSEDFAVYYCQQYNNW PFTFGGGTKVEIK SARS-CoV-2S2X30-VmAh CDRL(aa) 47 QSVSSN SARS-CoV-2S2X30-V1 GAS 169 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb CDRL2(aa)SARS-CoV-2S2X30-VmAb CDRL(aa) QQYNNWPFT SARS-CoV-2S2X30-VmAb VH (nt) GAGGTGCAGCTGGTGCAATCTGGGGCTGAG GTGAAGAAGCCTGGGTCCTCGGTGAAGGTC TCCTGCAAGGCTTCTGGAGGCACCTTCGG CAGCTATACTATCAGCTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAA GGATCATCCCTATCCTTAGTATACCAAAC TACGCACAGAAGTTCCAGGGCAGAGTCACG TTTACCGCGGACAAATCCACGAGCACAGCC TACATGGAGCTGAGCAGCCTGAGATCTGAG GACACGGCCGTGTATTACTGTGCGAGAGG AGGGGGTGGGACCCACGCAGTTCCCCAC TACTACTTTGACTCCTGGGGCCAGGGAAC CCTGGTCACCGTCTCCTCAG SARS-CoV-2S2X30-VmAb VL (nt) GAAATAGTGATGATGCAGTCTCCAGCCACC CTGTCTGTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTGTTAG CAGCAACTTAGCCTGGTACCAGCATAAACC TGGCCAGGCTCCCAGGCTCCTCATCTATGG TGCATCCACCAGGGCCACTGGTATCCCAGC CAGGTTCAGTGGCAGTGGGTCTGGGACAGA GTTCACTCTCACCATCAGCAGCATGCAGTC TGAAGATTTTGCAGTTTATTACTGTCAGCA GTATAATAACTGGCCTTTCACTTTCGGCG GAGGGACCAAGGTGGAGATCAAAC SARS-CoV-2S2X47-VmAb VH (aa) QVQLQESGPGLVKPSQTLSLTCTVSGGSISSR SYYWSWIRQPAGKGLEWIGRIYTSGNTNYNP SLRSRVTISVDTSKNQFSLKLGSVTAADTAVY YC ARERIIGTAH VGGWFDPWGQGTLVT VS S SARS-CoV-2S2X47-VmAbCDRH(aa) 53 GGSISSRSYY SARS-CoV-2S2X47-VmAbCDRH2 IYTSGNT 170 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence (aa)SARS-CoV-2S2X47-VmAb CDRH(aa) 55 ARERIIGTAHVGGWFDP SARS-CoV-2S2X47-VmAb VL (aa) QTVVTQPPSASGTPGQRVTISCSGSSSNIGSDTVNWYLQLPGTAPKLLIYTNNQRPSGVPDRFS GSKSGTSASLAISGLQSEDEANYYCAAWDDS LNGWVFGGGTKLTVL SARS-CoV-2S2X47-VmAbCDRL(aa) 57 SSNIGSDT SARS-CoV-2S2X47-VmAb CDRL(aa) TNN SARS-CoV-2S2X47-VmAb CDRL(aa) 59 AAWDDSLNGWV SARS-CoV-2S2X47-VmAb VH (nt) CAGGTGCAGCTACAGGAGTCGGGCCCAGG ACTGGTGAAGCCTTCACAGACCCTGTCCCT CACCTGCACTGTCTCTGGTGGCTCCATCAG CAGTCGAAGTTACTACTGGAGCTGGATCC GGCAGCCCGCCGGGAAGGGACTGGAGTGG ATTGGGCGTATCTATACCAGTGGCAACAC CAACTACAACCCCTCCCTCAGGAGTCGAGT CACCATATCAGTGGACACGTCCAAGAACCA GTTCTCCCTGAAGCTGGGCTCTGTGACCGC CGCAGACACGGCCGTGTATTATTGTGCGAG AGAGCGTATAATTGGAACCGCGCACGTG GGTGGGTGGTTCGACCCCTGGGGCCAGG GAACCCTGGTCACCGTCTCCTCAG SARS-CoV-2S2X47-VmAb VL (nt) CAGACTGTGGTGACTCAGCCACCCTCAGCG TCTGGGACCCCCGGGCAGAGGGTCACCATC TCTTGTTCTGGAAGCAGCTCCAACATCGG AAGTGATACTGTAAACTGGTACCTGCAGCT CCCAGGAACGGCCCCCAAACTCCTCATCTA TACTAATAATCAGCGGCCCTCAGGGGTCCC 171 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence TGACCGATTCTCTGGCTCCAAGTCTGGCAC CTCAGCCTCCCTGGCCATCAGTGGGCTCCA GTCTGAGGATGAGGCTAATTATTACTGTGC AGCATGGGATGACAGCCTGAATGGTTGG GTGTTCGGCGGAGGGACCAAGCTGACCGTC CTAG SARS-CoV-2S2X55-VmAb VH (aa) EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYMHWVRQAPGQGLEWMGWINPNSGAT NFAQKFQGRVTMTRDRSISTAYMELRSLRSD DTAVYYCARDLGDSRLSGWFDPWGQGTLV TVSS SARS-CoV-2S2X55-VmAbCDRH(aa) GYTFTGYY SARS-CoV-2S2X55-VmAbCDRH(aa) 64 INPNSGAT SARS-CoV-2S2X55-VmAbCDRH(aa) ARDLGDSRLSGWFDP SARS-CoV-2S2X55-VmAb VL (aa) QSALTQPASVSGSPGQSITISCTGTSSDVGSYN LVSWYQQHPGKAPKLMIYEGSKRPSGVSYRF SGSKSGNTASLTISGLQAEDEADYYCCSYAG SSTWVFGGGTKLTVL SARS-CoV-2S2X55-VmAbCDRL(aa) SSDVGSYNL SARS-CoV-2S2X55-VmAbCDRL(aa) 68 EGS SARS-CoV-2S2X55-VmAb CDRL3 CSYAGSSTWV 172 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence (aa) SARS-CoV-2S2X55-VmAb VH (nt) GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATATGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTGCCACAAACT TTGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACAGGTCCATCAGCACAGCCT ACATGGAGTTGAGAAGCCTGAGATCTGACG ACACGGCCGTTTATTACTGTGCGAGAGATC TCGGGGATAGCAGGTTGAGTGGCTGGTT CGACCCCTGGGGCCAGGGAACCCTGGTCA CCGTCTCCTCAG SARS-CoV-2S2X55-VmAb VL (nt) CAGTCTGCCCTGACTCAGCCTGCCTCCGTGT CTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAACCAGCAGTGATGTTGGG AGTTATAACCTTGTCTCCTGGTACCAACAG CACCCAGGCAAAGCCCCCAAACTCATGATT TATGAGGGCAGTAAGCGGCCCTCAGGGGT TTCTTATCGCTTCTCTGGCTCCAAGTCTGGC AACACGGCCTCCCTGACAATCTCTGGGCTC CAGGCTGAGGACGAGGCTGATTATTACTGC TGCTCATATGCAGGTAGTAGCACTTGGG TGTTCGGCGGAGGGACCAAGCTGACCGTCC TAG SARS-CoV-2S2X55-vmAb VH (aa) QVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYMHWVRQAPGQGLEWMGWINPNSGAT NFAQKFQGRVTMTRDRSISTAYMELRSLRSD DTAVYYCARDLGDSRLSGWFDPWGQGTLV TVSS SARS-CoV-S2X55-vmAb VH (nt) CAGGTCCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATATGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTGCCACAAACT TTGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACAGGTCCATCAGCACAGCCT ACATGGAGTTGAGAAGCCTGAGATCTGACG ACACGGCCGTTTATTACTGTGCGAGAGATC TCGGGGATAGCAGGTTGAGTGGCTGGTT 173 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CGACCCCTGGGGCCAGGGAACCCTGGTCA CCGTCTCCTCAG SARS-CoV-2S2X56-VmAb VH (aa) QVQLVQSGAEVKKPGSSVKVSCKASGGTFG SYTISWVRQAPGQGLEWMGRIIPILSIPNYAQ KFQGRVTFTADKSTSTAYMELSSLRSEDTAV YYCARGGGGTHAVPHYYFDSWGQGTLVTV SS SARS-CoV-2S2X56-VmAbCDRH(aa) GGTFGSYT SARS-CoV-2S2X56-VmAbCDRH(aa) 76 IIPILSIP SARS-CoV-2S2X56-VmAbCDRH(aa) ARGGGGTHAVPHYYFDS SARS-CoV-2S2X56-VmAb VL (aa) QTVLTQPASVSGSPGQSITISCTGTSSDVGSYNLVSWYQQHPGKAPKLMIYEGSKRPSGVSNRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGSSTHVFGTGTKVIVL SARS-CoV-2S2X56-VmAbCDRL(aa) SSDVGSYNL SARS-CoV-2S2X56-VmAbCDRL(aa) 80 EGS SARS-CoV-2S2X56-VmAb CDRL(aa) 81 CSYAGSSTHV SARS-CoV-2S2X56-V1CAGGTCCAGCTGGTGCAATCTGGGGCTGAG GTGAAGAAGCCTGGGTCCTCGGTGAAGGTC TCCTGCAAGGCTTCTGGAGGCACCTTCGG 174 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb VH (nt) CAGCTATACTATCAGCTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAA GGATCATCCCTATCCTTAGTATACCAAAC TACGCACAGAAGTTCCAGGGCAGAGTCACG TTTACCGCGGACAAATCCACGAGCACAGCC TACATGGAGCTGAGCAGCCTGAGATCTGAG GACACGGCCGTGTATTACTGTGCGAGAGG AGGGGGTGGGACCCACGCAGTTCCCCAC TACTACTTTGACTCCTGGGGCCAGGGAAC CCTGGTCACCGTCTCCTCAG SARS-CoV-2S2X56-VmAb VL (nt) CAGACTGTGCTGACTCAGCCTGCCTCCGTG TCTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAACCAGCAGTGATGTTGGG AGTTATAACCTTGTCTCCTGGTACCAACAG CACCCAGGCAAAGCCCCCAAACTCATGATT TATGAGGGCAGTAAGCGGCCCTCAGGGGT TTCTAATCGCTTCTCTGGCTCCAAGTCTGGC AACACGGCCTCCCTGACAATCTCTGGGCTC CAGGCTGAGGACGAGGCTGATTATTACTGC TGCTCATATGCAGGTAGTAGCACCCATG TCTTCGGAACTGGGACCAAGGTCATCGTCC TAG SARS-CoV-2S2X58-VmAb VH (aa) QVQLVQSGAEVKKPGASVKVSCKASGYTFT SYYMHWVRQAPGQGLEWMGIINRSGGSTSY AQKFQGRVTMTRDTSTSTVYMDLSSLRSEDT AVYYC ARDSGIAARVVYWGQGTLVTVS S SARS-CoV-2S2X58-VmAbCDRH(aa) GYTFTSYY SARS-CoV-2S2X58-VmAbCDRH(aa) 86 INRSGGST SARS-CoV-S2X58-VmAbCDRH(aa) ARDSGIAARVVY SARS-CoV-2S2X58-V1NIQMTQSPSSLSASVGDRVTITCQASQDISNY LNWYQQKPGKVPKLLIYDASNLETGVPSRFS 175 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAh VL (aa) GSGSGTDFTFTISSLQPEDIATYYCQQHDNLF TFGPGTKVDIK SARS-CoV-2S2X58-VmAhCDRL(aa)QDISNY SARS-CoV-2S2X58-VmAhCDRL(aa) 90 DAS SARS-CoV-2S2X58-VmAh CDRL(aa) QQHDNLFT SARS-CoV-2S2X58-VmAh VH (nt) CAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTT TCCTGCAAGGCATCTGGATACACCTTCAC CAGCTACTATATGCACTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAA TAATCAACCGTAGTGGTGGTAGCACAAG CTACGCACAGAAGTTCCAGGGCAGAGTCAC CATGACCAGGGACACGTCCACGAGCACAGT CTACATGGACCTGAGCAGCCTGAGATCTGA GGACACGGCCGTCTATTACTGTGCGAGAG ATTCGGGGATAGCAGCCAGGGTTGTCTA CTGGGGCCAGGGAACCCTGGTCACCGTCTC CTCAG SARS-CoV-2S2X58-VmAh VL (nt) AACATCCAGATGACCCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCAGGCGAGTCAGGACATTAG CAACTATTTAAATTGGTATCAGCAGAAACC AGGGAAAGTCCCTAAGCTCCTGATCTACGA TGCATCCAATTTGGAAACAGGGGTCCCATC AAGGTTCAGTGGAAGTGGATCTGGGACAGA TTTTACTTTCACCATCAGCAGCCTGCAGCCT GAAGATATTGCAACATATTACTGTCAACAG CATGATAATCTTTTCACTTTCGGCCCTGGG ACCAAAGTGGATATCAAAC SARS-CoV-S2X58-v2DIVMTQSPSSLSASVGDRVTITCQASQDISNY LNWYQQKPGKVPKLLIYDASNLETGVPSRFS 176 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb VL (aa) GSGSGTDFTFTISSLQPEDIATYYCQQHDNLF TFGPGTKVDIK SARS-CoV-S2X58-vmAb VL (nt) GACATCGTGATGACCCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCAGGCGAGTCAGGACATTAG CAACTATTTAAATTGGTATCAGCAGAAACC AGGGAAAGTCCCTAAGCTCCTGATCTACGA TGCATCCAATTTGGAAACAGGGGTCCCATC AAGGTTCAGTGGAAGTGGATCTGGGACAGA TTTTACTTTCACCATCAGCAGCCTGCAGCCT GAAGATATTGCAACATATTACTGTCAACAG CATGATAATCTTTTCACTTTCGGCCCTGGG ACCAAAGTGGATATCAAAC SARS-CoV-2S2X71-VmAb VH (aa) EVQLVESGGGLIQPGGSLRLSCAASGFTVSAN YMSWVRQTPGKGLEWVSVIYSGGSTYYADS VKGRFTISRDNSKNTLYLQMNSLRAEDTAVY YCARDLAAAAGPWGQGTLVTVSS SARS-CoV-2S2X71-VmAbCDRH(aa) 97 GFTVSANY SARS-CoV-2S2X71-VmAbCDRH(aa) IYSGGST SARS-CoV-S2X71-vmAb CDRH(aa) ARDLAAAAGP SARS-CoV-2S2X71-VmAb VL (aa)100 DIQMTQSPSSLSASVGDRVTITCRASQGIRND LGWFQQKPGKAPKLLIYAASSLQSGVPSRFSG SGSGTDFTLTISSLQPEDFATYYCLQDYNYPR TFGQGTKLEIK SARS-CoV-2S2X71-VmAb CDRLI (aa)101 QGIRND SARS-CoV-2 102 AAS 177 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2X71-VmAb CDRL(aa)SARS-CoV-2S2X71-VmAb CDRL(aa) 103 LQDYNYPRT SARS-CoV-2S2X71-VmAb VH (nt)104 GAGGTGCAGCTGGTGGAGTCTGGAGGAGG CTTGATCCAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGGTTCACCGTCAG TGCCAACTACATGAGCTGGGTCCGCCAGA CTCCAGGGAAGGGGCTGGAGTGGGTCTCAG TTATTTATAGCGGTGGTAGCACATACTAC GCAGACTCCGTGAAGGGCCGATTCACCATC TCCAGAGACAATTCCAAGAACACGCTGTAC CTTCAAATGAACAGCCTGAGAGCCGAGGAC ACGGCCGTGTATTACTGTGCGAGAGATTTA GCTGCAGCTGCTGGGCCCTGGGGCCAGG GAACCCTGGTCACCGTCTCCTCAG SARS-CoV-2S2X71-VmAb VL (nt)105 GACATCCAGATGACCCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCGGGCAAGTCAGGGCATTAG AAATGATTTAGGCTGGTTTCAGCAGAAACC AGGGAAAGCCCCTAAGCTCCTGATCTATGC TGCATCCAGTTTACAAAGTGGGGTCCCATC AAGGTTCAGCGGCAGTGGATCTGGCACAGA TTTCACTCTCACCATCAGCAGCCTGCAGCCT GAAGATTTTGCAACTTATTACTGTCTACAA GATTACAATTACCCTCGGACTTTTGGCCA GGGGACCAAGCTGGAGATCAAAC SARS-CoV-2S2X76-VmAb VH (aa)106 QVQLVQSGAEVKKPGASVKVSCKASGYSFT NYYMHWVRQAPGQGLEWMGIINASGGSTR YAQKFQGRVTMTRDTSTSTVYMELSSLRSED T AVYYC ARDSGIAARVVYWGQGTLVT VS S SARS-CoV-2S2X76-VmAbCDRH(aa)107 GYSFTNYY SARS-CoV-2S2X76-V1108 INASGGST 178 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb CDRH(aa)SARS-CoV-S2X76-vl mAbCDRH(aa)109 ARDSGIAARVVY SARS-CoV-2S2X76-VmAb VL (aa)110 AIRMTQSPSSLSASVGDRVTITCQASQDISNY LNWYQQKPGKAPKLLIYDASNLETGVPSRFS GSGSGTDFTFTISSLQPEDIATYYCQQHDDLF TFGPGTKVDIK SARS-CoV-2S2X76-VmAbCDRL(aa) 111 QDISNY SARS-CoV-2S2X76-VmAb CDRL(aa)112 DAS SARS-CoV-2S2X76-VmAb CDRL(aa) 113 QQHDDLFT SARS-CoV-2S2X76-VmAb VH (nt)114 caggtccagctggtgcagtctggggctgaggtgaagaagcctggggc ctcagtgaaggtttcctgcaaggcatctgggtacagcttcaccaacta ctatatgcactgggtgcgacaggcccctggacaagggcttgagtggat gggaataatcaacgctagtggtggtagcacaaggtacgcacagaag ttccagggcagagtcaccatgaccagggacacgtccacgagcacagt ctacatggagttgagcagcctgagatctgaggacacggccgtgtattac tgtgcgagagattcggggatagcagccagggttgtctactggggcc agggaaccctggtcaccgtctcctcag SARS-CoV-2S2X76-VmAb VL (nt)115 GCCATCCGGATGACCCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCAGGCGAGTCAGGACATTAG CAACTATTTAAATTGGTATCAGCAGAAACC AGGGAAAGCCCCTAAGCTCCTGATCTACGA TGCATCCAATTTGGAAACAGGGGTCCCATC AAGGTTCAGTGGAAGTGGATCTGGGACAGA TTTTACTTTCACCATCAGCAGCCTGCAGCCT GAAGATATTGCGACATATTACTGTCAACAA CATGATGATCTTTTCACTTTCGGCCCTGGG 179 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ACCAAAGTGGATATCAAAC SARS-CoV-2S2X76-vmAb VH (nt)116 caggtccagcttgtgcagtctggggctgaggtgaagaagcctggggc ctcagtgaaggtttcctgcaaggcatctgggtacagcttcaccaacta ctatatgcactgggtgcgacaggcccctggacaagggcttgagtggat gggaataatcaacgctagtggtggtagcacaaggtacgcacagaag ttccagggcagagtcaccatgaccagggacacgtccacgagcacagt ctacatggagttgagcagcctgagatctgaggacacggccgtgtattac tgtgcgagagattcggggatagcagccagggttgtctactggggcc agggaaccctggtcaccgtctcctcag SARS-CoV-2S2X76-vmAb VH (nt)117 caggtacagctggtgcagtctggggctgaggtgaagaagcctggggc ctcagtgaaggtttcctgcaaggcatctgggtacagcttcaccaacta ctatatgcactgggtgcgacaggcccctggacaagggcttgagtggat gggaataatcaacgctagtggtggtagcacaaggtacgcacagaag ttccagggcagagtcaccatgaccagggacacgtccacgagcacagt ctacatggagttgagcagcctgagatctgaggacacggccgtgtattac tgtgcgagagattcggggatagcagccagggttgtctactggggcc agggaaccctggtcaccgtctcctcag SARS-CoV-2S2X76-vmAb VH (nt)118 caggtacagcttgtgcagtctggggctgaggtgaagaagcctggggc ctcagtgaaggtttcctgcaaggcatctgggtacagcttcaccaacta ctatatgcactgggtgcgacaggcccctggacaagggcttgagtggat gggaataatcaacgctagtggtggtagcacaaggtacgcacagaag ttccagggcagagtcaccatgaccagggacacgtccacgagcacagt ctacatggagttgagcagcctgagatctgaggacacggccgtgtattac tgtgcgagagattcggggatagcagccagggttgtctactggggcc agggaaccctggtcaccgtctcctcag SARS-CoV-2S2X11-VmAb VH (aa)119 EVQLVESGGGLVQPGGSLRLSCAVSGFIVSSN YMTWVRQAPGKGLEWVSVIYSGGSTFYADS VKGRFTISRDNSKNTLYLQMNSLRAEDTAVY YC ARDFP VRRGVNIWGQGTLVT VS S SARS-CoV-2S2X11-VmAbCDRH(aa) 120 GFIVSSNY SARS-CoV-2S2X11-VmAbCDRH(aa)121 IYSGGST SARS-CoV-2122 ARDFPVRRGVNI 180 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence S2X11-VmAb CDRH(aa) SARS-CoV-2S2X11-VmAb VL (aa)123 DIQLTQSPSSLSASVGDRVTITCQASQDIVNYL NWYQQKPGKAPKLLIYDASNLETGVPSRFSG SGSGTDFTFTISSLQPEDIATYYCHQYDNLPP AF GGGTK VEIK SARS-CoV-2S2X11-VmAbCDRL(aa)124 QDIVNY SARS-CoV-2S2X11-VmAbCDRL(aa) 125 DAS SARS-CoV-2S2X11-VmAb CDRL(aa)126 HQYDNLPPA SARS-CoV-2S2X11-VmAb VH (nt)127 GAGGTGCAACTGGTGGAGTCTGGGGGAGG CTTGGTCCAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGTCTCTGGATTCATCGTCAG TAGCAACTACATGACTTGGGTCCGCCAGGC TCCAGGGAAGGGGCTGGAGTGGGTCTCAGT TATTTATAGCGGTGGTAGCACATTCTACG CAGACTCCGTGAAGGGCCGATTCACCATTT CCAGAGACAATTCCAAGAACACGCTGTATC TTCAAATGAACAGCCTGAGAGCTGAGGACA CGGCTGTGTATTATTGTGCGAGAGATTTCC CCGTACGTCGGGGAGTTAATATCTGGGGC CAGGGAACCCTGGTCACCGTCTCCTCAG SARS-CoV-2S2X11-VmAb VL (nt)128 GACATCCAGTTGACCCAGTCTCCATCCTCCC TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGCCAGGCGAGTCAGGACATTGTC AACTATTTAAATTGGTATCAACAGAAACCA GGGAAAGCCCCTAAGCTCCTGATCTACGAT GCATCCAATTTGGAAACAGGGGTCCCATCA AGGTTCAGTGGAAGTGGATCTGGGACAGAT TTTACTTTCACCATCAGCAGCCTGCAGCCTG AAGATATTGCAACATATTACTGTCACCAGT ATGATAATCTCCCTCCGGCTTTCGGCGGA 181 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GGGACCAAGGTGGAGATCAAAC SARS-CoV-2S2X35-VmAb VH (aa)129 QVQLVQSGAEVKKPGASVKVSCKASGYTFT NYGISWVRQAPGQGLEWMGWISAYKGNTN YAQKLQGRVTMTTDTSTSTAYMELRSLRSDD TAVYYCARPDYQVLGYDFWIGYYGMDVW GQGTTVIVSS SARS-CoV-2S2X35-VmAbCDRH(aa) 130 GYTFTNYG SARS-CoV-2S2X35-VmAbCDRH(aa)131 ISAYKGNT SARS-CoV-S2X35-vmAbCDRH(aa)132 ARPDYQVLGYDFWIGYYGMDV SARS-CoV-2S2X35-VmAb VL (aa)133 QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAG YDVHWYQQLPGTAPKLLIYGNTNRPSGVPDR FSGSKSGTSASLAITGLQAEDEADYYCQSYDS SLSGSEVVFGGGTKLTVL SARS-CoV-2S2X35-VmAbCDRL(aa)134 SSNIGAGYD SARS-CoV-2S2X35-VmAbCDRL(aa) 135 GNT SARS-CoV-2S2X35-VmAb CDRL(aa) 136 QSYDSSLSGSEVV SARS-CoV-2S2X35-VmAb VH (nt)137CAGGTCCAGCTGGTGCAGTCTGGAGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGTTACACCTTTACC AACTATGGTATCAGCTGGGTGCGACAGGC 182 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAGCGCTTACAAGGGTAACACAAACT ATGCACAGAAACTCCAGGGCAGAGTCACCA TGACCACAGACACATCCACGAGCACAGCCT ACATGGAGCTGAGGAGCCTGAGATCTGACG ACACGGCCGTGTATTACTGTGCGAGACCC GATTATCAGGTCCTGGGGTACGATTTTTG GATTGGGTACTACGGTATGGACGTCTGG GGCCAAGGGACCACGGTCATCGTCTCCTCA SARS-CoV-2S2X35-VmAb VL (nt)138 CAGTCTGTGTTGACGCAGCCGCCCTCAGTG TCTGGGGCCCCAGGGCAGAGGGTCACCATC TCCTGCACTGGGAGCAGCTCCAACATCGG GGCAGGTTATGATGTACACTGGTACCAGC AGCTTCCAGGAACAGCCCCCAAACTCCTCA TCTATGGTAACACCAATCGGCCCTCAGGGG TCCCTGACCGATTCTCTGGCTCCAAGTCTGG CACCTCAGCCTCCCTGGCCATCACTGGGCT CCAGGCTGAGGATGAGGCTGATTATTACTG CCAGTCTTATGACAGCAGCCTGAGTGGT TCGGAGGTGGTGTTCGGCGGAGGGACCAA GCTGACCGTCCTAG SARS-CoV-2S309 mAb VH (aa)139 QVQLVQSGAEVKKPGASVKVSCKASGYPFT SYGISWVRQAPGQGLEWMGWISTYNGNTNY AQKFQGRVTMTTDTSTTTGYMELRRLRSDDT AVYYCARDYTRGAWFGESLIGGFDNWGQG TLVTVSSSARS-CoV-S309 mAbCDRH1 (aa)140 GYPFTSYG SARS-CoV-S309 mAb CDRH2 (aa)141 ISTYNGNT SARS-CoV-S309 mAbCDRH3 (aa)142 ARDYTRGAWFGESLIGGFDN SARS-CoV-S309 mAb VL (aa)143EIVLTQSPGTLSLSPGERATLSCRASQTVSSTS LAWYQQKPGQAPRLLIYGASSRATGIPDRFSG SGSGTDFTLTISRLEPEDFAVYYCQQHDTSLT FGGGTKVEIKSARS-CoV-2S309 mAb144 QTVSSTS 183 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CDRLI (aa)SARS-CoV-S309 mAb CDRL2 (aa)145 GAS SARS-CoV-S309 mAbCDRL3 (aa)146 QQHDTSLT SARS-CoV-S2X16-vmAbCDRH(aa)147 GYIFTDHYMH SARS-CoV-S2X16-vmAbCDRH(aa)148 WINPNSGGTNYAQKFQG SARS-CoV-S2X16-vmAbCDRH(aa)149 DRSRFRFFSPDFDY SARS-CoV-2S2X16-vmAb VH (aa)150 EVQLVQSGAEVKKPGASVKVSCKASGYIFTD HYMHWVRQAPGQGLEWMGFINPNSGGTNY AQKFQGRVTMTRDTSINTAYMELSRLRSDDT AVHYCARDRSRFRFFSPDFDYWGQGTLVTV SS SARS-CoV-S2X16-vmAbCDRH(aa)151 FINPNSGGTNYAQKFQG SARS-CoV-S2X16-vmAbCDRLI (aa)152 RSSQSLLHSNGYNYLD SARS-CoV-S2X16-vmAbCDRL(aa)153 LGSNRAS SARS-CoV-S2X16-vmAb VL (aa)154EIVLTQSPLSLPVTPGEPASISCRSSQSLLHSQ GYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTPPTFGQGTKVEIK 184 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2X16-vmAb CDRL(aa)155 RSSQSLLHSQGYNYLD SARS-CoV-S2X16-vmAbCDRL(aa)156 QSLLHSQGYNY SARS-CoV-S2X16-vmAb VL (aa)157 EIVLTQSPLSLPVTPGEPASISCRSSQSLLHSN AYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTPPTFGQGTKVEIK SARS-CoV-S2X16-vmAbCDRL(aa)158 RSSQSLLHSNAYNYLD SARS-CoV-S2X16-vmAbCDRL(aa)159 QSLLHSNAYNY SARS-CoV-2S2X30-vmAbCDRH(aa) 160 GGTFGSYTIS SARS-CoV-S2X30-vmAbCDRH(aa)161 RIIPILSIPNYAQKFQG SARS-CoV-S2X30-vmAbCDRH(aa)162 GGGGTHAVPHYYFDS SARS-CoV-2S2X30-vmAb VH (aa)163 EVQLVQSGAEVKKPGSSVKVSCKASGGTFGS YTISWVRQAPGQGLEWMGRIIPILSIPNYAQK FQGRVTFTADKSTSTAYMELSSLRSEDTAVY YCARGGGGTHAVPHYYFESWGQGTLVTVS S SARS-CoV-2164 ARGGGGTHAVPHYYFES 185 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2X30-vmAhCDRH(aa)SARS-CoV-S2X30-vmAhCDRH(aa)165 GGGGTHAVPHYYFES SARS-CoV-S2X30-vmAhCDRL(aa)166 RASQSVSSNLA SARS-CoV-S2X30-vmAh CDRL(aa)167 GASTRAT SARS-CoV-S2X30-vmAh VH (aa)168 EIVMMQSPATLSVSPGERATLSCRASQSVSSN L AW YQHKPGQ APRLLIYGASTRATGIP ARF S GSGSGTEFTLTISSMQSEDFAVYYCQQYNNF PFTFGGGTKVEIK SARS-CoV-S2X30-vmAh CDRL(aa)169 QQYNNFPFT SARS-CoV-2S2X35-vmAhCDRH(aa)170 GYTFTNYGIS SARS-CoV-S2X35-vmAhCDRH(aa)171 WISAYKGNTNYAQKLQG SARS-CoV-S2X35-vmAhCDRH(aa)172 PDYQVLGYDFWIGYYGMDV SARS-CoV-2S2X35-vmAh VH (aa)173 QVQLVQSGAEVKKPGASVKVSCKASGYTFT NYGISWVRQAPGQGLEWMGFISAYKGNTNY AQKLQGRVTMTTDTSTSTAYMELRSLRSDDT AVYYCARPDYQVLGYDFWIGYYGMDVWG QGTTVIVSS 186 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2X35-vmAb CDRH(aa)174 FISAYKGNTNYAQKLQG SARS-CoV-2S2X35-vmAb VH (aa)175 QVQLVQSGAEVKKPGASVKVSCKASGYTFT NYGISWVRQAPGQGLEWMGWISAYKGNTN YAQKLQGRVTMTTDTSTSTAYMELRSLRSDD TAVYYCARPDYQVLGYDFFIGYYGMDVWG QGTTVIVSS SARS-CoV-2S2X35-vmAbCDRH(aa)176 ARPDYQVLGYDFFIGYYGMDV SARS-CoV-2S2X35-vmAbCDRH(aa) 177 PDYQVLGYDFFIGYYGMDV SARS-CoV-S2X35-vmAb VH (aa)178 QVQLVQSGAEVKKPGASVKVSCKASGYTFT NYGISWVRQAPGQGLEWMGFISAYKGNTNY AQKLQGRVTMTTDTSTSTAYMELRSLRSDDT AVYYCARPDYQVLGYDFFAGYYGMDVWG QGTTVIVSS SARS-CoV-S2X35-vmAbCDRH(aa)179 ARPDYQVLGYDFFAGYYGMDV SARS-CoV-S2X35-vmAbCDRH(aa)180 PDYQVLGYDFFAGYYGMDV SARS-CoV-S2X35-vmAbCDRL(aa)181 TGSSSNIGAGYDVH SARS-CoV-S2X35-vmAb CDRL(aa)182 GNTNRPS 187 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2X47-vmAb CDRH(aa)183 GGSISSRSYYWS SARS-CoV-S2X47-vmAbCDRH(aa)184 RIYTSGNTNYNPSLRS SARS-CoV-S2X47-vmAbCDRH(aa)185 ERIIGTAHVGGWFDP SARS-CoV-S2X47-vmAb VH (aa)186 QVQLQESGPGLVKPSQTLSLTCTVSGGSISSR SYYWSWIRQPAGKGLEWIGRIYTSGNTNYNP SLRSRVTISVDTSKNQFSLKLGSVTAADTAVY YCARERIIGTAHVGGFFDPWGQGTLVTVS S SARS-CoV-S2X47-vmAbCDRH(aa)187 ARERIIGTAHVGGFFDP SARS-CoV-S2X47-vmAbCDRH(aa)188 ERIIGTAHVGGFFDP SARS-CoV-S2X47-vmAb VH (aa)189 QVQLQESGPGLVKPSQTLSLTCTVSGGSISSR SYYF SWIRQP AGKGLEWIGRIYTSGNTNYNP SLRSRVTISVDTSKNQFSLKLGSVTAADTAVY YC ARERIIGTAH VGGWFDPWGQGTL VT VS S SARS-CoV-S2X47-vmAbCDRH(aa)190 GGSISSRSYYFS SARS-CoV-S2X47-vmAb VH (aa)191 QVQLQESGPGLVKPSQTLSLTCTVSGGSISSR SYYF SWIRQP AGKGLEWIGRIYTSGNTNYNP SLRSRVTISVDTSKNQFSLKLGSVTAADTAVY YCARERIIGTAHVGGFFDPWGQGTLVTVS S SARS-CoV-S2X47-vmAbCDRL1192 SGSSSNIGSDTVN 188 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence (aa)SARS-CoV-S2X47-vmAb CDRL(aa)193 TNNQRPS SARS-CoV-S2X47-vmAb VL (aa)194 QTVVTQPPSASGTPGQRVTISCSGSSSNIGSDTVNWYLQLPGTAPKLLIYTNNQRPSGVPDRFSGSKSGTSASLAISGLQSEDEANYYCAAFDDSLNGFVFGGGTKLTVL SARS-CoV-S2X47-vmAb CDRL(aa)195 AAFDDSLNGFV SARS-CoV-S2X47-vmAb VL (aa)196 QTVVTQPPSASGTPGQRVTISCSGSSSNIGSDTVNWYLQLPGTAPKLLIYTNNQRPSGVPDRFSGSKSGTSASLAISGLQSEDEANYYCAAFDDSLNGWVFGGGTKLTVL SARS-CoV-S2X47-vmAb CDRL(aa)197 AAFDDSLNGWV SARS-CoV-2S2H30-VmAb VH (aa)198 EVHLVESGGGLVQPGGSLRLSCAASGFIVSSN YMSWVRQAPGKGLEWVSVTYSGGSTFYADS VKGRFTISRDNSKNTVYLQMNSLRVEDTAVY YC ARDMGGQPGGYFDYWGQGTL VT VS S SARS-CoV-2S2H30-VmAbCDRH(aa)199 GFIVSSNY SARS-CoV-2S2H30-VmAbCDRH(aa)200 IYSGGST SARS-CoV-2S2H30-VmAbCDRH(aa) 201 ARDMGGQPGGYFDY SARS-CoV-2 202 DIVMTQSPSTLSASVGDRVTITCRASQSFSSW 189 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2H30-VmAb VL (aa)LAWYQQKPGKAPKLLIYKASNLESGVPSRFS GSGSGTEFTLTISSLQPDDFATYYCQQYNTYA TFGQGTKVEIK SARS-CoV-2S2H30-VmAbCDRL(aa) 203 QSFSSW SARS-CoV-2S2H30-VmAb CDRL(aa)204 KAS SARS-CoV-2S2H30-VmAb CDRL(aa) 205 QQYNTYAT SARS-CoV-2S2H30-VmAb VH (nt)206 GAGGTGCATCTGGTGGAGTCTGGGGGAGGC TTGGTCCAGCCTGGGGGGTCCCTGAGACTC TCCTGTGCAGCCTCTGGATTCATCGTCAGT AGCAACTACATGAGCTGGGTCCGCCAGGC TCCAGGGAAGGGGCTGGAGTGGGTCTCAGT TATTTATAGCGGTGGTAGTACATTCTACG CAGACTCCGTGAAGGGCAGATTCACCATCT CCAGAGACAATTCCAAGAACACGGTGTATC TTCAAATGAACAGCCTGAGAGTCGAGGACA CGGCTGTGTATTACTGTGCGCGGGACATG GGGGGGCAGCCTGGAGGCTACTTTGACT ACTGGGGCCAGGGAACCCTGGTCACCGTCT CCTCAG SARS-CoV-2S2H30-VmAb VL (nt)207 GACATCGTGATGACCCAGTCTCCTTCCACC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCGGGCCAGTCAGAGTTTTAGT AGCTGGTTGGCCTGGTATCAGCAGAAACC AGGGAAGGCCCCTAAGCTCCTGATCTATAA GGCATCTAATTTAGAAAGTGGGGTCCCATC AAGGTTCAGCGGCAGTGGATCTGGGACAGA ATTCACTCTCACTATCAGCAGCCTGCAGCCT GATGATTTTGCAACTTATTACTGCCAACAG TATAATACTTATGCGACGTTCGGCCAAGG GACCAAGGTGGAAATCAAGC 190 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-2S2H37-VmAb VH (aa)208 EVQLVESGGGLVQPGGSLRLSCAASGLTVSS NYMSWVRQAPGKGLEWVSLIYSGGTTYYA DSVKGRFTISRDNSKNTLYLQMNSLRTEDTA VYYC ARDPPHRSGGVWGQGTLVT VS S SARS-CoV-S2H37-vl mAbCDRH(aa)209 GLTVSSNY SARS-CoV-2S2H37-VmAbCDRH(aa)210 IYSGGTT SARS-CoV-2S2H37-VmAbCDRH(aa) 211 ARDPPHRSGGV SARS-CoV-2S2H37-VmAb VL (aa)212 AIWMTQSPSSLSASVGDRVTITCQASQDINNY LNWYQQKPGKAPKLLIYDASNLETGVPSRFS GSGSGTYFTFTISSLQPEDIATYYCQQSDNLPI TFGQGTRLEIK SARS-CoV-2S2H37-VmAbCDRL(aa) 213 QDINNY SARS-CoV-2S2H37-VmAb CDRL(aa)214 DAS SARS-CoV-2S2H37-VmAb CDRL(aa) 215 QQSDNLPIT SARS-CoV-2S2H37-VmAb VH (nt)216 GAGGTGCAGCTGGTGGAGTCTGGGGGAGGT TTGGTCCAGCCTGGGGGGTCCCTGAGACTC TCCTGTGCAGCCTCTGGATTGACCGTCAGT AGCAACTACATGAGCTGGGTCCGCCAGGC TCCAGGGAAGGGGCTGGAGTGGGTCTCACT TATTTATAGCGGTGGTACCACATACTACG CAGACTCCGTGAAGGGCCGATTCACCATCT 191 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CCAGAGACAATTCCAAGAACACACTATATC TTCAAATGAACAGCCTGAGAACTGAGGACA CGGCTGTGTATTACTGTGCGAGAGACCCT CCCCACCGCAGTGGCGGGGTCTGGGGCC AGGGAACCCTGGTCACCGTCTCCTCAG SARS-CoV-2S2H37-VmAb VL (nt)217 GCCATCTGGATGACCCAGTCTCCATCCTCCC TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGCCAGGCGAGTCAGGACATTAAC AACTATTTAAATTGGTATCAGCAGAAACCA GGGAAAGCCCCTAAACTCCTGATCTACGAT GCATCCAATTTGGAAACAGGGGTCCCATCA AGGTTCAGTGGAAGTGGATCTGGGACATAT TTTACTTTCACCATCAGCAGCCTGCAGCCTG AAGATATTGCAACATATTACTGTCAACAGT CTGATAATCTCCCGATCACATTCGGCCAA GGGACACGACTGGAGATTAAAC SARS-CoV-2S2H40-VmAb VH (aa)218 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYMHWVRQAPGQGLEWMGWINPISGATN YAQKFQGRVTMTRDTSISTAYMELSRLRSDD TAVYYCARVASSGWYELEILYGVDVWGQG TTVTVSS SARS-CoV-S2H40-VmAbCDRH(aa)219 GYTFTGYY SARS-CoV-S2H40-VmAbCDRH(aa)220 INPISGAT SARS-CoV-S2H40-VmAbCDRH(aa)221 ARVASSGWYELEILYGVDV SARS-CoV-S2H40-VmAb VL (aa)222 QSVLTQPASVSGSPGQSITISCTGTSSDVGGY NYVSWYQQHPGKAPKLMIYEVSNRPSGVSN RFSGSKSGNTASLTISGLQAEDEADYYCSSYT SSSTQVFGTGTKVTVL SARS-CoV-2S2H40-V1223 SSDVGGYNY 192 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb CDRL(aa)SARS-CoV-S2H40-VmAbCDRL(aa)224 EVS SARS-CoV-S2H40-VmAb CDRL(aa)225 SSYTSSSTQV SARS-CoV-2S2H40-VmAb VH (nt)226 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATATGCACTGGGTGCGACAGGC CCCTGGACAGGGGCTTGAGTGGATGGGATG GATCAACCCTATCAGTGGTGCCACAAACT ATGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACACGTCCATCAGCACAGCCT ACATGGAGCTGAGCAGGCTGAGATCTGACG ACACGGCCGTGTATTACTGTGCGAGAGTA GCTAGCAGTGGCTGGTATGAACTGGAAA TTCTCTACGGTGTGGACGTCTGGGGCCAA GGGACCACGGTCACCGTCTCCTCA SARS-CoV-S2H40-VmAb VL (nt)227 CAGTCTGTGCTGACTCAGCCTGCCTCCGTGT CTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAACCAGCAGTGACGTTGGT GGTTATAACTATGTCTCCTGGTACCAACAG CACCCAGGCAAAGCCCCCAAACTCATGATT TATGAGGTCAGTAATCGGCCCTCAGGGGTT TCTAATCGCTTCTCTGGCTCCAAGTCTGGCA ACACGGCCTCCCTGACCATCTCTGGGCTCC AGGCTGAGGACGAGGCTGATTATTACTGCA GCTCATATACAAGCAGCAGCACTCAGGT CTTCGGAACTGGGACCAAGGTCACCGTCCT AG SARS-CoV-2S2H58-VmAb VH (aa)228 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGWINPNSGGTN F AQKFQGRVTMTRATSF STAYMELS SLRSDD TAVYYCASSGYLGYYYYGMDVWGQGTTVT vss 193 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2H58-VmAb CDRH(aa)229 GYTFTGYY SARS-CoV-S2H58-VmAbCDRH(aa)230 INPNSGGT SARS-CoV-S2H58-VmAbCDRH(aa)231 ASSGYLGYYYYGMDV SARS-CoV-2S2H58-VmAb VL (aa)232 ATWMTQSPLSLPVTPGEPASISCRSSQSLLHSN VYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTPHTFGQGTKLEIK SARS-CoV-S2H58-VmAbCDRL(aa)233 QSLLHSNVYNY SARS-CoV-S2H58-VmAbCDRL(aa)234 LGS SARS-CoV-S2H58-VmAb CDRL(aa)235 MQALQTPHT SARS-CoV-S2H58-VmAb VH (nt)236 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATATACACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTGGCACAAACT TTGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGCCACGTCCTTCAGCACAGCCT ACATGGAGCTGAGCAGTCTGAGATCTGACG ACACGGCCGTGTATTACTGTGCGAGTAGT GGTTACCTCGGTTATTACTACTACGGTAT GGACGTCTGGGGCCAAGGGACCACGGTCA CCGTCTCCTCA 194 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2H58-VmAb VL (nt)237 GCCATCTGGATGACCCAGTCTCCACTCTCCC TGCCCGTCACCCCTGGAGAGCCGGCCTCCA TCTCCTGCAGGTCTAGTCAGAGCCTCCTGC ATAGTAATGTATACAACTATTTGGATTGGT ACCTGCAGAAGCCAGGGCAGTCTCCACAGC TCCTGATCTATTTGGGTTCTAATCGGGCCT CCGGGGTCCCTGACAGGTTCAGTGGCAGTG GATCAGGCACAGATTTTACACTGAAAATCA GCAGAGTGGAGGCTGAGGATGTTGGGGTTT ATTACTGCATGCAAGCTCTACAAACTCCT CACACTTTTGGCCAGGGGACCAAGCTGGA GATCAAAC SARS-CoV-S2H58-vmAb VL (aa)238 DIVMTQTPLSLPVTPGEPASISCRSSQSLLHSN VYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTPHTFGQGTKLEIK SARS-CoV-S2H58-vmAb VL (nt)239 GATATTGTGATGACCCAGACTCCACTCTCC CTGCCCGTCACCCCTGGAGAGCCGGCCTCC ATCTCCTGCAGGTCTAGTCAGAGCCTCCTG CATAGTAATGTATACAACTATTTGGATTG GTACCTGCAGAAGCCAGGGCAGTCTCCACA GCTCCTGATCTATTTGGGTTCTAATCGGGC CTCCGGGGTCCCTGACAGGTTCAGTGGCAG TGGATCAGGCACAGATTTTACACTGAAAAT CAGCAGAGTGGAGGCTGAGGATGTTGGGGT TTATTACTGCATGCAAGCTCTACAAACTC CTCACACTTTTGGCCAGGGGACCAAGCTGG AGATCAAAC SARS-CoV-2S2H62-vl mAb VH (aa)240 EVHLVESGGGLVQPGGSLRLSCAASVITVSSN YMSWVRQAPGKGLEWVSLIYSGGSTYYADS VKGRFTISRDNFKNTLYLQMNSLRAEDTAVY YC ARDLDIAGGMDVWGQGTT VT VS S SARS-CoV-S2H62-vl mAbCDRH(aa)241 VITVSSNY SARS-CoV-2S2H62-vl mAbCDRH2242 IYSGGST 195 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence (aa)SARS-CoV-S2H62-vl mAhCDRH(aa)243 ARDLDIAGGMDV SARS-CoV-S2H62-vl mAh VL (aa)244 DIVMTQTPSSLSASVGDRVTITCRAGQTISNY LNWYQQKPGKAPKLLIYAASSLQSGVPSRFS GSGSGTDFTLTISSLQPEDFATYYCQQSYSTP YTFGQGTKLEIK SARS-CoV-S2H62-vl mAhCDRL(aa)245 QTISNY SARS-CoV-S2H62-vl mAhCDRL(aa)246 AAS SARS-CoV-S2H62-vl mAh CDRL(aa)247 QQSYSTPYT SARS-CoV-S2H62-vl mAh VH (nt)248 GAGGTGCATCTGGTGGAGTCTGGGGGAGGC TTGGTCCAGCCTGGGGGGTCCCTGAGACTC TCCTGTGCAGCCTCTGTAATCACCGTCAGT AGCAACTACATGAGCTGGGTCCGCCAGGC TCCAGGGAAGGGGCTGGAGTGGGTCTCACT TATTTATAGCGGTGGTAGCACATACTACG CAGACTCCGTGAAGGGCCGATTCACCATCT CCAGAGACAATTTCAAGAACACGCTGTATC TTCAAATGAACAGCCTGAGAGCTGAGGACA CGGCTGTGTATTACTGTGCGAGAGATCTG GATATAGCAGGCGGTATGGACGTCTGGG GCCAAGGGACCACGGTCACCGTCTCCTCA SARS-CoV-S2H62-vl mAh VL (nt)249 gatattgtgatgacccagactccatcctccctgtctgcatctgtaggaga cagagtcaccatcacttgccgggcaggtcagaccattagcaactattt aaattggtatcagcagaaaccagggaaagcccctaagctcctgatctat gctgcatccagtttgcaaagtggggtcccatcaaggttcagtggcagtg gatctgggacagatttcactctcaccatcagcagtctgcaacctgaagat tttgcaacttactactgtcaacagagttacagtaccccgtacacttttgg ccaggggaccaagctggagatcaaac 196 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2H62-vmAb VL (aa)250 DIGMTQTPSSLSASVGDRVTITCRAGQTISNY LNWYQQKPGKAPKLLIYAASSLQSGVPSRFS GSGSGTDFTLTISSLQPEDFATYYCQQSYSTP YTFGQGTKLEIK SARS-CoV-S2H62-vmAb VL (nt)251 gatattgggatgacccagactccatcctccctgtctgcatctgtaggaga cagagtcaccatcacttgccgggcaggtcagaccattagcaactattta aattggtatcagcagaaaccagggaaagcccctaagctcctgatctatg ctgcatccagtttgcaaagtggggtcccatcaaggttcagtggcagtgg atctgggacagatttcactctcaccatcagcagtctgcaacctgaagattt tgcaacttactactgtcaacagagttacagtaccccgtacacttttggcca ggggaccaagctggagatcaaac SARS-CoV-S2H62-vmAb VL (aa)252 DIQLTQSPSSLSASVGDRVTITCRAGQTISNYL NWYQQKPGKAPKLLIYAASSLQSGVPSRFSG SGSGTDFTLTISSLQPEDFATYYCQQSYSTPY TFGQGTKLEIK SARS-CoV-S2H62-vmAb VL (nt)253 gacatccagttgacccagtctccatcctccctgtctgcatctgtaggaga cagagtcaccatcacttgccgggcaggtcagaccattagcaactattt aaattggtatcagcagaaaccagggaaagcccctaagctcctgatctat gctgcatccagtttgcaaagtggggtcccatcaaggttcagtggcagtg gatctgggacagatttcactctcaccatcagcagtctgcaacctgaagat tttgcaacttactactgtcaacagagttacagtaccccgtacacttttgg ccaggggaccaagctggagatcaaa SARS-CoV-2S2H66-VmAb VH (aa)254 EVQLQESGPGLVKPSETLSLTCSVSGGSISSY YWS WIRQPPGK ALEWIGYIYYSGSTNYNS SL KSRVTISLDTSKNQF SLKLS S VAAADT AVYYC ARHDPFRA VTGHFDYWGQGTLVTVS S SARS-CoV-S2H66-VmAbCDRH(aa)255 GGSISSYY SARS-CoV-S2H66-VmAbCDRH(aa)256 IYYSGST SARS-CoV-S2H66-VmAb CDRH(aa)257 ARHDPFRA VTGHFDY SARS-CoV-2258 QTVLTQPHSVSESPGKTVTISCTRSSGSIASNY 197 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2H66-VmAb VL (aa)VQWYQQRPGSSPTIVIYEDSRRPSGVPDRFSG SIDSSSNSASLSISRLKTEDEADYYCQSYYRN TVVFGGGTKLTVL SARS-CoV-S2H66-VmAbCDRL(aa)259 SGSIASNY SARS-CoV-S2H66-VmAb CDRL(aa)260 EDS SARS-CoV-S2H66-VmAb CDRL(aa)261 QSYYRNTVV SARS-CoV-2S2H66-VmAb VH (nt)262 GAGGTGCAGCTGCAGGAGTCGGGCCCAGG ACTGGTGAAGCCTTCGGAGACCCTGTCCCT CACCTGCTCTGTCTCTGGTGGCTCCATCAG TAGTTACTACTGGAGCTGGATCCGGCAGCC CCCAGGGAAGGCACTGGAGTGGATTGGATA TATCTATTACAGTGGGAGCACCAACTACA ATTCATCCCTCAAGAGTCGAGTCACCATAT CACTAGACACGTCCAAGAACCAGTTCTCCC TGAAGCTGAGCTCTGTGGCCGCCGCAGACA CGGCCGTGTATTACTGTGCGAGACATGAC CCTTTCAGGGCAGTGACTGGGCACTTTG ACTACTGGGGCCAGGGAACCCTGGTCACCG TCTCCTCAG SARS-CoV-S2H66-VmAb VL (nt)263 cagactgtgctgactcagccccactctgtgtcggagtctccggggaag acggtaaccatctcctgcacccgcagcagtggcagcattgccagcaa ctatgtgcagtggtaccaacagcgcccgggcagttcccccaccattgt gatctatgaggatagccgaagaccctctggggtccctgatcggttctct ggctccatcgacagctcctccaactctgcctccctcagcatctctagact gaagactgaggacgaggctgactactactgtcagtcttattatagaaa cactgtggtattcggcggagggaccaagctgaccgtcctag SARS-CoV-2S2H70-VmAb VH (aa)264 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYLHWVRQAPGQGLEWMGWINPISGGTN YAQKFQGRVTMTRDTSISTAYMELSRLRSDD TAVYYCARVDYDILTGYYWGWFDPWGQG TLVTVSS 198 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2H70-VmAb CDRH(aa)265 GYTFTGYY SARS-CoV-S2H70-VmAbCDRH(aa)266 INPISGGT SARS-CoV-S2H70-VmAbCDRH(aa)267 ARVDYDILTGYYWGWFDP SARS-CoV-S2H70-VmAb VL (aa)268 QTVVTQPASVSGSPGQSITISCTGTSSDVGGY NYVSWYQQHPGKAPKLMIYDVSNRPSGVSN RFSGSKSGNTASLTISGLQAEDEADYYCSSYT SSSTYVFGTGTKVTVL SARS-CoV-S2H70-VmAbCDRL(aa)269 SSDVGGYNY SARS-CoV-S2H70-VmAb CDRL(aa)270 DVS SARS-CoV-S2H70-VmAb CDRL(aa)271 SSYTSSSTYV SARS-CoV-S2H70-VmAb VH (nt)272 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATCTGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTATCAGTGGTGGCACAAACT ATGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACACGTCCATCAGCACAGCCT ACATGGAGCTGAGCAGGCTGAGATCTGACG ACACGGCCGTGTATTACTGTGCGAGAGTG GATTACGATATTTTGACTGGTTATTACTG GGGCTGGTTCGACCCCTGGGGCCAGGGA 199 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ACCCTGGTCACCGTCTCCTCAG SARS-CoV-S2H70-VmAb VL (nt)273 CAGACTGTGGTGACTCAGCCTGCCTCCGTG TCTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAACCAGCAGTGACGTTGGT GGTTATAACTATGTCTCCTGGTACCAACAG CACCCAGGCAAAGCCCCCAAACTCATGATT TATGATGTCAGTAATCGGCCCTCAGGGGTT TCTAATCGCTTCTCTGGCTCCAAGTCTGGCA ACACGGCCTCCCTGACCATCTCTGGGCTCC AGGCTGAGGACGAGGCTGATTATTACTGCA GCTCATATACAAGCAGCAGCACTTATGTC TTCGGAACTGGGACCAAGGTCACCGTCCTA G SARS-CoV-2S2H71-vl mAb VH (aa)274 QITLKESGPTLVKPTQTLTLTCTFSGFSLSTGG VGVGWIRQPPGKALEWLALIYWDDDKRYSP SLKSRLTITKDTSKNQVVLTMTNVDPVDTATYYC ARHT VTRIFDYWGQGTL VT VS S SARS-CoV-S2H71-vl mAbCDRH(aa)275 GFSLSTGGVG SARS-CoV-S2H71-vl mAbCDRH(aa)276 IYWDDDK SARS-CoV-S2H71-vl mAbCDRH(aa)277 ARHTVTRIFDY SARS-CoV-S2H71-vl mAb VL (aa)278 QSVLTQPASVSGSPGQSITISCTGTSSDVGGFL YVSWYQQLPGKAPKLMIYEVSDRPSGVSNRF SGSKSGNTASLTISGLQAEDEADYYCSSYTTS STVFGGGTKLTVL SARS-CoV-S2H71-vl mAb CDRL(aa)279 SSDVGGFLY SARS-CoV-2S2H71-vl280 EVS 200 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb CDRL2(aa)SARS-CoV-S2H71-vl mAb CDRL(aa)281 SSYTTSSTV SARS-CoV-S2H71-vl mAb VH (nt)282 CAGATCACCTTGAAGGAGTCTGGTCCTACG CTGGTGAAACCCACACAGACCCTCACGCTG ACCTGCACCTTCTCTGGGTTCTCACTCAGC ACTGGTGGAGTGGGTGTGGGCTGGATCCG TCAGCCCCCAGGAAAGGCCCTGGAGTGGCT TGCACTCATTTATTGGGATGATGATAAGC GCTACAGCCCATCTCTGAAGAGCAGGCTCA CCATCACCAAGGACACTTCCAAAAACCAGG TGGTCCTTACAATGACCAACGTGGACCCTG TGGACACAGCCACATATTACTGTGCACGCC ATACGGTGACTAGGATATTTGACTACTGG GGCCAGGGAACCCTGGTCACCGTCTCCTCA G SARS-CoV-S2H71-vl mAb VL (nt)283 CAGTCTGTGCTGACTCAGCCTGCCTCCGTGT CTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAACCAGCAGTGACGTTGGT GGTTTTCTCTATGTCTCCTGGTACCAACAA CTCCCAGGCAAAGCCCCCAAACTCATGATT TATGAGGTCAGTGATCGGCCCTCAGGGGTT TCTAATCGCTTCTCTGGCTCCAAGTCTGGCA ACACGGCCTCCCTGACCATCTCTGGGCTCC AGGCTGAGGACGAGGCTGATTATTACTGCA GCTCATATACAACCAGCAGCACGGTGTTC GGCGGAGGGACCAAGTTGACCGTCCTAG SARS-CoV-2S2H73-vl mAb VH (aa)284 QVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGWINPNSGGTN YAQKFQGRVTMTRDTSISTAYMELSRLRSDD TAVYYCARELLVPGAMSPYGMDVWGQGTT VTVSS SARS-CoV-S2H73-vmAbCDRH(aa)285 GYTFTGYY SARS-CoV-2286 INPNSGGT 201 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2H73-VmAb CDRH(aa)SARS-CoV-S2H73-vl mAbCDRH(aa)287 ARELLVPGAMSPYGMDV SARS-CoV-S2H73-vl mAb VL (aa)288 EIVMTQSPATLSLSPGERATLSCRASQSVSRY L AW YQQKPGQ APRLLI YD ASNRAAGIP ARF S GSGSGTDFTLTISSLEPEDFVVYYCQQRSNWP PLAFGGGTKVEIK SARS-CoV-S2H73-vl mAbCDRL(aa)289 QSVSRY SARS-CoV-S2H73-vl mAbCDRL(aa)290 DAS SARS-CoV-S2H73-vl mAb CDRL(aa)291 QQRSNWPPLA SARS-CoV-S2H73-vl mAb VH (nt)292 CAGGTCCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATATACACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTGGCACAAACT ATGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACACGTCCATCAGCACAGCCT ACATGGAGCTGAGCAGGCTGAGATCTGACG ACACGGCCGTGTATTACTGTGCGAGGGAA TTATTAGTACCAGGTGCTATGTCCCCTTA CGGTATGGACGTCTGGGGCCAAGGGACCA CGGTCACCGTCTCCTCA SARS-CoV-S2H73-vl mAb VL (nt)293 GAAATAGTGATGACGCAGTCTCCAGCCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTGTTAG CAGGTACTTAGCCTGGTACCAACAGAAAC CTGGCCAGGCTCCCAGGCTCCTCATCTATG 202 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ATGCATCCAACAGGGCCGCTGGCATCCCA GCCAGGTTCAGTGGCAGTGGGTCTGGGACA GACTTCACTCTCACCATCAGCAGCCTAGAG CCTGAAGATTTTGTAGTTTATTACTGTCAGC AGCGTAGCAACTGGCCTCCGCTCGCTTTC GGCGGAGGGACCAAGGTGGAGATCAAAC SARS-CoV-S2H66-vmAb VL (aa)294SYELTQPHSVSESPGKTVTISCTRSSGSIASNY VQWYQQRPGSSPTIVIYEDSRRPSGVPDRFSG SIDSSSNSASLSISRLKTEDEADYYCQSYYRN TVVFGGGTKLTVL SARS-CoV-S2H66-vmAb VL (nt)295 tcctatgagctgacacagccccactctgtgtcggagtctccggggaag acggtaaccatctcctgcacccgcagcagtggcagcattgccagcaac tatgtgcagtggtaccaacagcgcccgggcagttcccccaccattgtga tctatgaggatagccgaagaccctctggggtccctgatcggttctctgg ctccatcgacagctcctccaactctgcctccctcagcatctctagactga agactgaggacgaggctgactactactgtcagtcttattatagaaacact gtggtattcggcggagggaccaagctgaccgtccta SARS-CoV-S2H66-vmAb VL (aa)296 QPVLTQPHSVSESPGKTVTISCTRSSGSIASNY VQW YQQRPGS SPTIVIYEDSRRPSGVPDRF SG SIDSSSNSASLSISRLKTEDEADYYCQSYYRN TVVFGGGTKLTVL SARS-CoV-S2H66-vmAb VL (nt)297 Cagcctgtgctgactcagccccactctgtgtcggagtctccggggaag acggtaaccatctcctgcacccgcagcagtggcagcattgccagcaac tatgtgcagtggtaccaacagcgcccgggcagttcccccaccattgtga tctatgaggatagccgaagaccctctggggtccctgatcggttctctgg ctccatcgacagctcctccaactctgcctccctcagcatctctagactga agactgaggacgaggctgactactactgtcagtcttattatagaaacact gtggtattcggcggagggaccaagctgaccgtccta SARS-CoV-2S2N12-VmAb VH (aa)298 QVRLQESGPGLVKPSETLSLTCTVSGGSISSSTYFWGWIRQPPGKGLEWIGSISYSGSTYYNPSL KSRVTISVDTSKSQFSLKLSSVTAADTAVYYC ARRGGYCSRVNCYNRYWYFDLWGRGTLVT vss SARS-CoV-S2N12-VmAbCDRH(aa)299 GGSISSSTYF SARS-CoV-2300 ISYSGST 203 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2N12-VmAb CDRH(aa)SARS-CoV-S2N12-VmAbCDRH(aa)301 ARRGGYCSRVNCYNRYWYFDL SARS-CoV-2S2N12-VmAb VL (aa)302 DIQLTQSPSSVSASVGDRVTVSCRASQDISSW LAWYQQKPGKAPKLLIYAASSLQSGVPSRFS GSGSGTDFTLTISSLQPEDFATYFCQQANSFP VTFGPGTKVDIK SARS-CoV-S2N12-VmAbCDRL(aa)303 QDISSW SARS-CoV-S2N12-VmAbCDRL(aa)304 AAS SARS-CoV-S2N12-VmAb CDRL(aa)305 QQANSFPVT SARS-CoV-2S2N12-VmAb VH (nt)306 CAGGTGCGGCTGCAGGAGTCGGGCCCAGG ACTGGTGAAGCCTTCGGAGACCCTGTCCCT CACCTGCACTGTCTCTGGTGGCTCCATCAG CAGTAGTACTTACTTCTGGGGCTGGATCC GCCAGCCCCCAGGGAAGGGGCTGGAGTGG ATTGGGAGTATCTCTTATAGTGGGAGCAC CTACTACAACCCGTCCCTCAAGAGTCGAGT CACCATATCCGTTGACACGTCCAAGAGCCA GTTCTCCCTGAAGCTGAGCTCTGTGACCGC CGCAGACACGGCTGTGTATTACTGTGCGAG ACGGGGGGGATATTGTAGTCGTGTTAAC TGCTACAATCGCTACTGGTACTTCGATCT CTGGGGCCGTGGCACCCTGGTCACTGTCTC CTCAG SARS-CoV-S2N12-VmAb VL (nt)307GACATCCAGTTGACGCAGTCTCCATCTTCTG TGTCTGCATCTGTAGGAGACAGAGTCACCG TCAGTTGTCGGGCGAGTCAGGATATTAGC AGCTGGTTAGCCTGGTATCAGCAGAAACC 204 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AGGGAAAGCCCCTAAGCTCCTGATCTATGC TGCATCCAGTTTGCAAAGTGGGGTCCCATC AAGGTTCAGCGGCAGTGGATCTGGGACAGA TTTCACTCTCACTATCAGCAGCCTGCAGCCT GAAGATTTTGCAACTTACTTTTGTCAACAG GCTAACAGTTTCCCTGTCACTTTCGGCCCTG GGACCAAAGTGGATATCAAAC SARS-CoV-S2N12-vmAb VL (aa)308 DIVMTQSPSSVSASVGDRVTVSCRASQDISSW LAWYQQKPGKAPKLLIYAASSLQSGVPSRFS GSGSGTDFTLTISSLQPEDFATYFCQQANSFP VTFGPGTKVDIK SARS-CoV-S2N12-vmAb VL (nt)309 GACATCGTGATGACCCAGTCTCCATCTTCTG TGTCTGCATCTGTAGGAGACAGAGTCACCG TCAGTTGTCGGGCGAGTCAGGATATTAGC AGCTGGTTAGCCTGGTATCAGCAGAAACC AGGGAAAGCCCCTAAGCTCCTGATCTATGC TGCATCCAGTTTGCAAAGTGGGGTCCCATC AAGGTTCAGCGGCAGTGGATCTGGGACAGA TTTCACTCTCACTATCAGCAGCCTGCAGCCT GAAGATTTTGCAACTTACTTTTGTCAACAG GCTAACAGTTTCCCTGTCACTTTCGGCCC TGGGACCAAAGTGGATATCAAAC SARS-CoV-S2N12-vmAb VL (aa)310 NIQMTQSPSSVSASVGDRVTVSCRASQDISSW LAWYQQKPGKAPKLLIYAASSLQSGVPSRFS GSGSGTDFTLTISSLQPEDFATYFCQQANSFP VTFGPGTKVDIK SARS-CoV-S2N12-vmAb VL (nt)311 AACATCCAGATGACCCAGTCTCCATCTTCT GTGTCTGCATCTGTAGGAGACAGAGTCACC GTCAGTTGTCGGGCGAGTCAGGATATTAG CAGCTGGTTAGCCTGGTATCAGCAGAAAC CAGGGAAAGCCCCTAAGCTCCTGATCTATG CTGCATCCAGTTTGCAAAGTGGGGTCCCAT CAAGGTTCAGCGGCAGTGGATCTGGGACAG ATTTCACTCTCACTATCAGCAGCCTGCAGCC TGAAGATTTTGCAACTTACTTTTGTCAACA GGCTAACAGTTTCCCTGTCACTTTCGGCC CTGGGACCAAAGTGGATATCAAAC SARS-CoV-2S2N22-vl312EVQLVESGGGLVQPGGSLRLSCAASGFTFSS YPMSWVRQAPGKGLEWVSAISGSGNYTYYA DSVKGRFTISRDNSKNTLYLQMNSLRAEDTA 205 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAh VH (aa) VYYC AKDPGGLSNFDFWGQGTL VTVS S SARS-CoV-S2N22-vl mAhCDRH(aa)313 GFTFSSYP SARS-CoV-S2N22-vl mAhCDRH(aa)314 ISGSGNYT SARS-CoV-S2N22-vl mAhCDRH(aa)315 AKDPGGLSNFDF SARS-CoV-S2N22-vl mAh VL (aa)316 NIQMTQSPSSLSASVGDRVTITCRASQSISTYL NWFQQKPGKAPKLLIYAASSLQSGVPSRFSGS GSGTDFTLTISSLQREDFATYYCQQTYSTPRI FGQGTKLEIK SARS-CoV-S2N22-vl mAhCDRL(aa)317 QSISTY SARS-CoV-S2N22-vl mAhCDRL(aa)318 AAS SARS-CoV-S2N22-vl mAh CDRL(aa)319 QQTYSTPRI SARS-CoV-S2N22-vl mAh VH (nt)320 GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CTTGGTACAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGATTCACCTTTAG CAGTTATCCTATGAGCTGGGTCCGCCAGGC TCCAGGTAAGGGGCTGGAGTGGGTCTCAGC TATTAGTGGTAGTGGTAATTACACATACT ACGCAGACTCCGTGAAGGGCCGGTTCACCA TCTCCAGAGACAATTCCAAGAACACGTTGT ATCTGCAAATGAACAGCCTGAGAGCCGAGG ACACGGCCGTATATTACTGTGCGAAAGATC CCGGTGGGTTGTCGAACTTTGACTTCTGG 206 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GGCCAGGGAACCCTGGTCACCGTCTCCTCA G SARS-CoV-S2N22-vl mAb VL (nt)321 AACATCCAGATGACCCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCGGGCAAGTCAGAGCATTAG CACCTATTTAAATTGGTTTCAGCAGAAACC AGGGAAAGCCCCTAAGCTCCTGATCTATGC TGCATCCAGTTTGCAAAGTGGGGTCCCGTC AAGGTTCAGTGGCAGTGGATCTGGGACAGA TTTCACTCTCACCATCAGCAGTCTACAACGT GAAGATTTTGCAACTTACTACTGTCAACAG ACTTACAGTACCCCTCGAATTTTTGGCCA GGGGACCAAGCTGGAGATCAAAC SARS-CoV-2S2N25-VmAb VH (aa)322 QVQLQESGPGLVKPSETLSLTCTVSGGSISSSYWSWIRQPPGKGLEWIGYLYYSGSTNYNPSL KSRVTISVDTSKNQFSLKLTSVTAADTAVYYC ARDPGPYYYDSSGYYLDAFDIWGQGTMVTV ss SARS-CoV-S2N25-VmAbCDRH(aa)323 GGSISSSY SARS-CoV-S2N25-VmAbCDRH(aa)324 LYYSGST SARS-CoV-S2N25-VmAbCDRH(aa)325 ARDPGPYYYDSSGYYLDAFD SARS-CoV-S2N25-VmAb VL (aa)326 ATWMTQSPSSLSASVGDRVTITCRASQSIVSY LNWYQQKPGKAPKLLIYAASSLQSGVPSRFS GSGSGTDFTLTISSLQPEDFATYYCQQTYNTL SLTFGGGTKVEIK SARS-CoV-S2N25-VmAb CDRL(aa)327 QSIVSY 207 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence SARS-CoV-S2N25-VmAb CDRL(aa)328 AAS SARS-CoV-S2N25-VmAb CDRL(aa)329 QQTYNTLSLT SARS-CoV-S2N25-VmAb VH (nt)330 CAGGTGCAGCTGCAGGAGTCGGGCCCAGG ACTGGTGAAGCCTTCGGAGACCCTGTCCCT CACCTGCACTGTCTCTGGTGGCTCCATCAG TAGTTCCTACTGGAGCTGGATCCGGCAGCC CCCAGGGAAGGGACTGGAGTGGATTGGCTA TCTCTATTACAGTGGGAGCACCAACTACA ACCCCTCCCTCAAGAGTCGAGTCACCATAT CAGTAGACACGTCCAAGAACCAGTTCTCCC TGAAGCTGACCTCTGTGACCGCTGCGGACA CGGCCGTGTATTACTGTGCGAGAGACCCC GGGCCGTATTACTATGATAGTAGTGGTT ATTACCTGGATGCTTTTGATATCTGGGGC CAAGGGACAATGGTCACCGTCTCTTCAG SARS-CoV-S2N25-VmAb VL (nt)331 GCCATCTGGATGACCCAGTCTCCATCCTCCC TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGCCGGGCAAGTCAGAGCATTGTC AGCTATTTAAATTGGTATCAGCAGAAACCA GGGAAAGCCCCTAAGCTCCTGATCTATGCT GCATCCAGTTTGCAAAGTGGGGTCCCATCA AGGTTCAGTGGCAGTGGATCTGGGACAGAT TTCACTCTCACCATCAGCAGTCTGCAACCTG AAGATTTTGCAACTTACTACTGTCAACAGA CTTACAATACCCTTTCGCTCACTTTCGGC GGAGGGACCAAGGTGGAGATCAAAC SARS-CoV-2S2N28-VmAb VH (aa)332 EVQLVESGGGVVQPGRSLRLSCAASGFTFFS YGMHWVRQAPGKGLEWVAFIRYDGSNKYY ADSVKGRFTISRDNSKNTLYLQMNSLRAEDT AVYYCAKDSPRVTSGGYYSYYYGMDVWG QGTTVTVSS SARS-CoV-2S2N28-VmAbCDRH1333 GFTFFSYG 208 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence (aa)SARS-CoV-S2N28-VmAb CDRH(aa)334 IRYDGSNK SARS-CoV-S2N28-VmAbCDRH(aa)335 AKDSPRVTSGGYYSYYYGMDV SARS-CoV-2S2N28-VmAb VL (aa)336 DIVMTQTPSSLSASVGDRVTITCRASQNISSYL NWYQQKPRKAPKLLIYPASSLQSGVPSRFSGS GSGTDFTLTISSLQPEDFATYYCQQSYSTPLT FGGGTKVEIK SARS-CoV-S2N28-VmAbCDRL(aa)337 QNISSY SARS-CoV-S2N28-VmAbCDRL(aa)338 PAS SARS-CoV-S2N28-VmAb CDRL(aa)339 QQSYSTPLT SARS-CoV-S2N28-VmAb VH (nt)340 GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CGTGGTCCAGCCTGGGAGGTCCCTGAGACT CTCCTGTGCAGCGTCTGGATTCACCTTCTT TAGCTATGGAATGCACTGGGTCCGCCAGG CCCCGGGCAAGGGGCTGGAGTGGGTGGCAT TTATACGGTATGATGGAAGTAATAAATAC TATGCAGACTCCGTGAAGGGCCGATTCACC ATCTCCAGAGACAATTCCAAGAACACGCTG TATCTGCAAATGAACAGCCTGAGAGCTGAG GACACAGCTGTGTATTACTGTGCGAAAGAC TCTCCTCGGGTTACCAGTGGCGGATATT ACTCCTACTACTACGGTATGGACGTCTGG GGCCAAGGGACCACGGTCACCGTCTCCTCA SARS-CoV-2S2N28-V1341GATATTGTGATGACCCAGACTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC 209 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb VL (nt) ATCACTTGCCGGGCAAGTCAGAACATTAG CAGTTATTTAAATTGGTATCAGCAGAAACC ACGGAAAGCCCCTAAACTCCTGATCTATCC TGCATCCAGTTTGCAAAGTGGGGTCCCATC AAGGTTCAGTGGCAGTGGATCTGGGACAGA TTTCACTCTCACCATCAGCAGTCTGCAACCT GAAGATTTTGCAACTTACTACTGTCAACAG AGTTACAGTACGCCGCTCACTTTCGGCGG AGGGACCAAGGTGGAGATCAAAC SARS-CoV-2S309-v2 mAb VH(aa)342 QVQLVQSGAEVKKPGASVKVSCKASGYPFT SYGISWVRQAPGQGLEWMGWISTYQGNTN YAQKFQGRVTMTTDTSTTTGYMELRRLRSD DTAVYYCARDYTRGAWFGESLIGGFDNWG QGTLVTVSSSARS-CoV-2S309-v2 mAb CDRH1 (aa)343 GYPFTSYG SARS-CoV-S309-v2 mAbCDRH2 (aa)344 ISTYQGNT SARS-CoV-2S309-v2 mAb CDRH3 (aa)345 ARDYTRGAWFGESLIGGFDN SARS-CoV-2S309-v2 mAb VL (aa)346EIVLTQSPGTLSLSPGERATLSCRASQTVSSTS LAWYQQKPGQAPRLLIYGASSRATGIPDRFSG SGSGTDFTLTISRLEPEDFAVYYCQQHDTSLT FGGGTKVEIKSARS-CoV-2S309-v2 mAbCDRL1 (aa)347 QTVSSTS SARS-CoV-2S309-v2 mAbCDRL2 (aa)348 GAS SARS-CoV-2S309-v2 mAbCDRL3 (aa)349 QQHDTSLT SARS-CoV-S2H58-vmAb VH (aa)350 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGFINPNSGGTNF AQKFQGRVTMTRATSF STAYMELS SLRSDDT AVYYCASSGYLGYYYYGMDVWGQGTTVTV SS 210 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2H58-VmAb VH (aa)351 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGFINPNAGGTNF AQKFQGRVTMTRATSF STAYMELS SLRSDDT AVYYCASSGYLGYYYYGMDVWGQGTTVTV SS SARS-CoV-S2H58-vmAbCDRH(aa)352 INPNAGGT SARS-CoV-2S2H58-vmAb VH (aa)353 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGFINPASGGTNF AQKFQGRVTMTRATSF STAYMELS SLRSDDT AVYYCASSGYAGYYYYGMDVWGQGTTVTV SS SARS-CoV-S2H58-vmAbCDRH(aa)354 ASSGYAGYYYYGMDV SARS-CoV-S2H58-vmAb VL (aa)355 DIVMTQTPLSLPVTPGEPASISCRSSQSLLHSN GYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTPHTFGQGTKLEIK SARS-CoV-S2H58-vmAbCDRL(aa)356 QSLLHSNGYNY SARS-CoV-S2H58-VmAb VL (aa)357 DIVMTQTPLSLPVTPGEPASISCRSSQSLLHSN VYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCIQA LQTPHTFGQGTKLEIK SARS-CoV-S2H58-VmAb CDRL(aa)358 IQALQTPHT SARS-CoV-2S2N22-vmAb VH (aa)359EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYPMSWVRQAPGKGLEWVSAISGSGQYTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYC AKDPGGLSNFDFWGQGTL VTVS S 211 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2N22-vmAb CDRH(aa)360 ISGSGQYT SARS-CoV-2S2N22-vmAb VH (aa)361 EVQLVESGGGLVQPGGSLRLSCAASGFTFSS YPMSWVRQAPGKGLEWVSAISGSGGYTYYA DSVKGRFTISRDNSKNTLYLQMNSLRAEDTA VYYCAKDPGGLSNFDFWGQGTLVTVS S SARS-CoV-S2N22-vmAbCDRH(aa)362 ISGSGGYT SARS-CoV-2S2N22-vmAb VH (aa)363 EVQLVESGGGLVQPGGSLRLSCAASGFTFSS YPMSWVRQAPGKGLEWVSAISGSGNYAYYA DSVKGRFTISRDNSKNTLYLQMNSLRAEDTA VYYCAKDPGGLSNFDFWGQGTLVTVS S SARS-CoV-S2N22-vmAbCDRH(aa)364 ISGSGNYA SARS-CoV-S2N22-vmAb VH (aa)365 EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYPMSWVRQAPGKGLEWVSAISGSGNPTYYA DSVKGRFTISRDNSKNTLYLQMNSLRAEDTA VYYCAKDPGGLSNFDFWGQGTLVTVS S SARS-CoV-S2N22-vmAbCDRH(aa)366 ISGSGNPT SARS-CoV-S2N22-vmAb VH (aa)367 EVQLVESGGGLVQPGGSLRLSCAASGFTFSS YPMSWVRQAPGKGLEWVSAISGSGNYAYYA DAVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDPGGLSNFDFWGQGTLVTVS S SARS-CoV-S2N22-vmAb VH (aa)368 EVQLVESGGGLVQPGGSLRLSCAASGFTFSS YPISWVRQAPGKGLEWVSAISGSGNYAYYA DAVKGRFTISRDNSKNTLYLQMNSLRAEDTA VYYCAKDPGGLSNFDFWGQGTLVTVS S 212 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence SARS-CoV-2S2E6-vl mAb VH(aa)369QITLKESGPTLVKPTQTLTLTCTFSGFSLSTSG VGVGWIRQPPGEALEWLALIFWDDDKRYRP SLKSRLTITKDTSKNQVVLTMTNMDPVDTAT YYC AHRRTS YGS YYFD YWGQGTL VT VS SSARS-CoV-2S2E6-v1 mAbCDRH1 (aa)370 GFSLSTSGVG SARS-CoV-2S2E6-v1 mAbCDRH2 (aa)371 IFWDDDK SARS-CoV-2S2E6-v1 mAbCDRH3 (aa)372 AHRRTSYGSYYFDY SARS-CoV-2S2E6-v1 mAbVL (aa)373DIEMTQSPSTLSASVGDRVTITCRASQSISNW LAWYQQKPGRAPKLLIYRASNLESGVPSRFS GSGSGTEFTLTISSLQPDDFATYYCQQYNSYP TFGQGTKVEIKSARS-CoV-S2E6-v1 mAb CDRL1 (aa)374 QSISNW SARS-CoV-2S2E6-v1 mAb CDRL2 (aa)375 RAS SARS-CoV-S2E6-v1 mAb CDRL3 (aa)376 QQYNSYPT SARS-CoV-2S2E6-v1 mAbVH(nt)377 CAGATCACCTTGAAGGAGTCTGGTCCTACG CTGGTGAAACCCACACAGACCCTCACGCTG ACCTGCACCTTCTCTGGGTTCTCACTCAGC ACTAGTGGAGTGGGTGTGGGCTGGATCCG TCAGCCCCCAGGAGAGGCCCTGGAGTGGCT TGCACTCATTTTTTGGGATGATGATAAGC GCTACAGGCCATCTCTGAAGAGCAGGCTCA CCATCACCAAGGACACCTCCAAAAACCAGG TGGTCCTTACAATGACCAACATGGACCCTG TGGACACAGCCACATATTACTGTGCACACA GACGTACCAGCTATGGTTCTTACTACTTT GACTATTGGGGCCAGGGAACCCTGGTCACC GTCTCCTCAGSARS-CoV-2S2E6-v1 mAbVL(nt)378GACATCGAGATGACCCAGTCTCCTTCCACC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCGGGCCAGTCAGAGTATTAGT 213 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AACTGGTTGGCCTGGTATCAGCAGAAACCA GGGAGAGCCCCTAAGCTCCTAATCTATAGG GCGTCTAATTTAGAAAGTGGGGTCCCATCA AGGTTCAGCGGCAGTGGATCTGGGACAGAA TTCACTCTCACCATCAGCAGCCTGCAGCCT GATGATTTTGCAACTTATTACTGCCAACAG TATAATAGTTATCCGACGTTCGGCCAAGG GACCAAGGTGGAAATCAAACSARS-CoV-2S2E7-V1 mAb VH(aa)379EVQLVESGGGLVQPGGSLRLSCAASGLTVSS NYMTWVRQAPGKGLEWVSIIYSGGSTFYAD SVKGRFTISRDNSKNTLNLQMNSLRVEDTAV YYCARPIVGSRAGMDVWGQGTTVTVSSSARS-CoV-2S2E7-V1 mAbCDRH1 (aa)380 GLTVSSNY SARS-CoV-S2E7-V1 mAb CDRH2 (aa)381 IYSGGST SARS-CoV-2S2E7-V1 mAbCDRH3 (aa)382 ARPIVGSRAGMDV SARS-CoV-2S2E7-V1 mAb VH(aa)383EIVMTQSPSSLSASVGDRVTITCQASQDINKY LNWYQQKPGKAPKLLIYDASNLETGVPSRFS GSGSGTDFAFTISSLQPEDVATYYCHQYDNL PLTFGGGTKVEIKSARS-CoV-2S2E7-V1 mAbCDRL1 (aa)384 QDINKY SARS-CoV-2S2E7-V1 mAbCDRL2 (aa)385 DAS SARS-CoV-2S2E7-V1 mAbCDRL3 (aa)386 HQYDNLPLT SARS-CoV-2S2E7-V1 mAb VH(nt)387 GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CTTGGTCCAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGATTAACTGTCAG TAGCAACTACATGACCTGGGTCCGCCAGG CTCCAGGGAAGGGGCTGGAGTGGGTCTCAA TTATTTATAGCGGTGGTAGCACATTCTAC GCAGACTCCGTGAAGGGCCGATTCACCATC TCCAGAGACAATTCCAAGAACACGCTGAAT 214 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CTTCAAATGAACAGCCTGAGAGTTGAGGAC ACGGCTGTGTATTACTGTGCGAGACCCATA GTGGGATCCAGAGCCGGTATGGACGTCT GGGGCCAAGGGACCACGGTCACCGTCTCCT CA SARS-CoV-S2E7-V1 mAb VL(nt)388 GAAATAGTGATGACGCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCAGGCGAGTCAAGACATTAA CAAGTATTTAAATTGGTATCAGCAGAAACC AGGGAAAGCCCCTAAACTCCTTATCTACGA TGCATCCAATTTGGAAACAGGGGTCCCATC AAGGTTCAGTGGAAGTGGGTCTGGGACAGA TTTTGCTTTCACCATCAGCAGCCTGCAGCCT GAAGATGTTGCAACATATTACTGTCACCAG TATGATAATCTCCCGCTCACTTTCGGCGG AGGGACCAAGGTGGAGATCAAACSARS-CoV-2S2E9-V1 mAb VH(aa)389EVQLVESGGGLIQPGGSLRLSCAASGLTVSSN YMSWVRQAPGKGLEWVSVTYSGGSTFYAES VKGRFTISRDNSKNTLYLQLNSLRAADTAVY YCARDLEIHGMDVWGQGTTVTVSSSARS-CoV-2S2E9-V1 mAbCDRH1 (aa)390 GLTVSSNY SARS-CoV-S2E9-V1 mAb CDRH2 (aa)391 IYSGGST SARS-CoV-2S2E9-V1 mAbCDRH3 (aa)392 ARDLEIHGMDV SARS-CoV-S2E9-V1 mAbVL (aa)393EIVLTQSPSFLSASVGDRVTITCRASQGISNYL AWYQQKPGKAPQLLIYAASTLQSGVPSRFSG SGSGTEFTLTISSLQPEDFATYYCQQLNSYPP VTFGPGTKVDIKSARS-CoV-2S2E9-V1 mAbCDRL1 (aa)394 QGISNY SARS-CoV-2S2E9-V1 mAbCDRL2 (aa)395 AAS SARS-CoV-2S2E9-V1 mAbCDRL3 (aa)396 QQLNSYPPVT 215 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2E9-V1 mAh VH(nt)397 GAGGTGCAGCTGGTGGAGTCTGGAGGAGG CTTGATCCAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGGTTAACCGTCAG TAGCAACTACATGAGCTGGGTCCGCCAGG CTCCAGGGAAGGGGCTGGAGTGGGTCTCAG TTATTTATAGCGGTGGTAGCACATTCTAC GCAGAATCCGTGAAGGGCCGATTCACCATC TCCAGAGACAATTCCAAGAACACGCTGTAT CTTCAATTGAACAGCCTGAGAGCCGCGGAC ACGGCCGTGTATTACTGTGCGAGAGATCTT GAGATTCACGGTATGGACGTCTGGGGCCA AGGGACCACGGTCACCGTCTCCTCA SARS-CoV-S2E9-V1 mAh VL(nt)398 GAAATTGTGCTGACTCAGTCTCCATCCTTCC TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGCCGGGCCAGTCAGGGCATTAGC AATTATTTAGCCTGGTATCAGCAAAAACCA GGGAAAGCCCCTCAGCTCCTGATCTATGCT GCATCCACTTTGCAAAGTGGGGTCCCATCA AGGTTCAGCGGCAGTGGATCTGGGACAGAA TTCACTCTCACAATCAGCAGCCTGCAGCCT GAAGATTTTGCAACTTATTACTGTCAACAG CTTAATAGTTACCCTCCGGTCACTTTCGG CCCTGGGACCAAAGTGGATATCAAAC SARS-CoV-S2E12-vmAh VH (aa)399 QVQLVQSGPEVKKPGTSVRVSCKASGFTFTS SAVQWVRQARGQRLEWVGWIVVGSGNTNY AQKFHERVTITRDMSTSTAYMELSSLRSEDTA VYYCASPYCSGGSCSDGFDIWGQGTMVTVS S SARS-CoV-S2E12-vmAh CDRH(aa)400 GFTFTSSA SARS-CoV-S2E12-vmAhCDRH(aa)401 IVVGSGNT SARS-CoV-S2E12-vmAh CDRHand N-terminal Al a-Ser(aa) 402 ASPYCSGGSCSDGFDI SARS-CoV-2403 DIVLTQTPGTLSLSPGERATLSCRASQSVSSSY 216 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2E12-VmAb VL (aa)LAWYQQKPGQAPRLLTYGASSRATGIPDRFSG SGSGTDFTLTISRLEPEDFAVYYCQQYVGLT GWTFGQGTKVEIK SARS-CoV-S2E12-vmAb CDRLI (aa)404 QSVSSSY SARS-CoV-S2E12-vmAb CDRL(aa)405 GAS SARS-CoV-S2E12-vmAb CDRL(aa)406 QQYVGLTGWT SARS-CoV-S2E12-vmAb VH (nt)407 CAGGTGCAGCTGGTGCAGTCTGGGCCTGAG GTGAAGAAGCCTGGGACCTCAGTGAGGGTC TCCTGCAAGGCTTCTGGATTCACCTTTACT AGCTCTGCTGTACAGTGGGTGCGACAGGC TCGTGGACAACGCCTTGAGTGGGTGGGATG GATCGTCGTTGGCAGTGGTAACACAAACT ACGCACAGAAGTTCCACGAAAGAGTCACCA TTACCAGGGACATGTCCACAAGCACAGCCT ACATGGAGCTGAGCAGCCTGAGATCCGAGG ACACGGCCGTGTATTACTGTGCGTCACCTT ATTGTAGTGGTGGTAGCTGCTCTGATGG TTTTGATATCTGGGGCCAAGGGACAATGG TCACCGTCTCTTCAG SARS-CoV-S2E12-vmAb VL (nt)408 GATATTGTGTTGACGCAGACTCCAGGCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTGTTAG CAGCAGTTACTTAGCCTGGTACCAGCAGA AACCTGGCCAGGCTCCCAGGCTCCTCATCT ATGGTGCATCCAGCAGGGCCACTGGCATC CCAGACAGGTTCAGTGGCAGTGGGTCTGGG ACAGACTTCACTCTCACCATCAGCAGACTG GAGCCTGAAGATTTTGCAGTGTATTACTGT CAGCAGTATGTTGGCTTAACAGGGTGGA CGTTCGGCCAAGGGACCAAGGTGGAAATC AAAC 217 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence SARS-CoV-2S2E13-vl mAb VH (aa)409 QVQLVQSGTEVKKPGASVKVSCKASGYTFIS YGISWVRQAPGQGLEWMGWISPYNGNTHY AKRVQGRVTMTTDTSTNTSYMELRSLRSDDT AVYYCARDGELLGWFDPWGQGTLVTVSS SARS-CoV-S2E13-vmAbCDRH(aa)410 GYTFISYG SARS-CoV-S2E13-vmAbCDRH(aa)411 ISPYNGNT SARS-CoV-S2E13-vmAbCDRH(aa)412 ARDGELLGWFDP SARS-CoV-S2E13-vmAb VL (aa)413 HTELTQPASVSGSPGQSITISCTGTSSDVGNYN LVSWYQQHPGKAPKLMIYAGTKRPSGVSNR FSGSKSGNTASLTISGLQAEDEADYYCCSYA GSSTWVFGGGTKLTVL SARS-CoV-S2E13-vmAb CDRLI (aa)414 SSDVGNYNL SARS-CoV-S2E13-vmAb CDRL(aa)415 AGT SARS-CoV-S2E13-vmAb CDRL(aa)416 CSYAGSSTWV SARS-CoV-S2E13-vmAb VH (nt)417 CAGGTGCAGCTGGTGCAGTCTGGAACTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGTTACACCTTTATC AGCTATGGTATCAGCTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAGCCCTTACAATGGTAACACACATT ATGCAAAGAGGGTCCAGGGCAGAGTCACC ATGACCACAGACACATCCACGAACACATCC 218 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence TACATGGAGCTGAGGAGCCTGAGATCTGAC GACACGGCCGTGTATTACTGTGCGAGAGA TGGAGAACTCCTCGGCTGGTTCGATCCC TGGGGCCAGGGAACCCTGGTCACCGTCTCC TCAG SARS-CoV-S2E13-vl mAb VL (nt)418 CACACTGAGCTGACTCAGCCTGCCTCCGTG TCTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAACCAGCAGTGATGTTGGG AATTATAACCTTGTCTCCTGGTACCAACAG CACCCAGGCAAAGCCCCCAAACTCATGATT TATGCGGGCACTAAGCGGCCCTCAGGGGT TTCTAATCGCTTCTCTGGCTCCAAGTCTGGC AACACGGCCTCCCTGACAATCTCTGGGCTC CAGGCTGAGGACGAGGCTGACTATTACTGC TGCTCATATGCAGGTAGTAGCACTTGGG TGTTCGGCGGAGGGACCAAGCTGACCGTCC TAG SARS-CoV-2S2E14-VmAb VH (aa)419 EVQLVESGGGVVQPGRSLRLSCAASGFTFSS YAMHWVRQAPGKGLEWVTVISSDGSNKYY AESVKGRFTISRDNSKNTLYLQMNSLRAEDT ALYYCARDLPVIVATMWHYYNGMDVWGQ GTTVTVSSSARS-CoV-S2E14-VmAb CDRH(aa)420 GFTFSSYA SARS-CoV-2S2E14-VmAb CDRH(aa)421 ISSDGSNK SARS-CoV-S2E14-VmAb CDRH(aa)422 ARDLPVIVATMWHYYNGMDV SARS-CoV-2S2E14-VmAb VL (aa)423DIRMTQSPSSVSASVGDRVTITCRASEGISSW LGWYQQKPGKAPKLLIYGASSLQSGVPSRFS GSGFGTDFTLTISSLQPEDFATYYCQQAKSFP ITFGQGTRLEIKSARS-CoV-2S2E14-VmAb CDRL1424 EGISSW 219 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence (aa)SARS-CoV-S2E14-VmAb CDRL(aa)425 GAS SARS-CoV-S2E14-VmAb CDRL(aa)426 QQAKSFPIT SARS-CoV-2S2E14-VmAb VH (nt)427 GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CGTGGTCCAGCCTGGGAGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGATTCACCTTCAG TAGCTATGCTATGCACTGGGTCCGCCAGGC TCCAGGCAAGGGGCTGGAGTGGGTGACAGT TATTTCATCTGATGGAAGCAATAAATACT ACGCGGAGTCCGTGAAGGGCCGATTCACCA TCTCCAGAGACAATTCCAAGAACACGCTGT ATCTGCAAATGAACAGCCTGAGAGCTGAGG ACACGGCTCTATATTACTGTGCGAGAGACC TTCCTGTTATAGTGGCTACGATGTGGCAC TACTATAACGGAATGGACGTCTGGGGCCA AGGGACCACGGTCACCGTCTCCTCG SARS-CoV-2S2E14-VmAb VL (nt)428 GACATCCGGATGACCCAGTCTCCATCTTCC GTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGTCGGGCGAGTGAAGGTATTAG CAGCTGGTTAGGCTGGTATCAGCAGAAAC CAGGGAAGGCCCCTAAACTCCTGATCTACG GTGCATCCAGTTTGCAAAGTGGGGTCCCAT CAAGGTTCAGCGGCAGTGGATTTGGGACAG ATTTCACTCTCACCATCAGCAGTCTGCAGCC TGAAGATTTTGCAACTTACTATTGTCAACA GGCTAAGAGTTTCCCGATCACCTTCGGCC AAGGGACACGACTGGAGATTAAAC SARS-CoV-2S2K4-V1 mAb VH(aa)429 QVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYMHWVRQAPGQGLEWMGWINPNSGGT NYTQKFQGWVTMTRDTSISTAYMELSRLSSD DTAVYYCARDLAYSYVTGAFDIWGHGTMV TVSSSARS-CoV-2S2K4-V1 mAbCDRH1 (aa)430 GYTFTGYY SARS-CoV-2431 INPNSGGT 220 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2K4-V1 mAb CDRH2 (aa)SARS-CoV-S2K4-V1 mAb CDRH3 (aa)432 ARDLAYSYVTGAFDI SARS-CoV-S2K4-V1 mAb VH(nt)433 CAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTATATGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTGGCACAAACT ATACACAGAAGTTTCAGGGCTGGGTCACCA TGACCAGGGACACGTCCATCAGCACAGCCT ACATGGAGCTGAGCAGGCTGAGCTCTGACG ACACGGCCGTGTATTACTGTGCGAGAGAT CTCGCATACAGCTATGTCACGGGGGCTT TTGATATCTGGGGCCACGGGACAATGGTC ACCGTCTCTTCAG SARS-CoV-S2X193-VmAb VH (aa)434 EVHLVESGGGLVQPGGSLRLSCAASGIIVSRN YMSWVRQAPGKGLEWVSVIYSGGSTFYADS VKGRFTISRDNSKNTLYLQMNSLRAGDTAVY YCARDMMNDAFDIWGQGTMVTVSS SARS-CoV-S2X193-VmAbCDRH(aa)435 GIIVSRNY SARS-CoV-S2X193-VmAbCDRH(aa)436 IYSGGST SARS-CoV-S2X193-VmAbCDRH(aa)437 ARDMMNDAFDI SARS-CoV-S2X193-VmAb VH (aa)438 DIQMTQSPSSLSASVGDRVTITCRASQGISSYL AWYQQKPGKAPKLLIYSASTLQSGVPSRFSGS GSGTDFTLTISSLQPEDFATYYCQQLNSYPPE VTFGQGTRLEIK SARS-CoV-2S2X193-V1439 QGISSY 221 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb CDRL(aa)SARS-CoV-S2X193-VmAbCDRL(aa)440 SAS SARS-CoV-S2X193-VmAb CDRL(aa)441 QQLNSYPPEVT SARS-CoV-S2X193-VmAb VH (nt)442 GAGGTGCATCTGGTGGAGTCTGGGGGAGGT TTGGTCCAGCCGGGGGGGTCCCTGAGACTC TCCTGTGCAGCCTCTGGAATCATCGTCAGT AGGAATTACATGAGCTGGGTCCGCCAGGC TCCAGGGAAGGGGCTGGAGTGGGTCTCAGT TATTTATAGCGGTGGTAGTACATTCTACG CAGACTCCGTGAAGGGCCGATTCACCATCT CCAGAGACAATTCCAAGAACACGCTGTATC TTCAAATGAACAGCCTGAGAGCTGGGGACA CGGCTGTGTATTACTGTGCGAGGGATATG ATGAATGATGCTTTTGATATCTGGGGCCA AGGGACAATGGTCACCGTCTCTTCAG SARS-CoV-S2X193-VmAb VL (nt)443 GACATCCAGATGACCCAGTCTCCATCCTCC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCGGGCCAGTCAGGGCATTAG CAGTTATTTAGCCTGGTATCAGCAAAAACC AGGGAAAGCCCCTAAGCTCCTGATCTATTC TGCATCCACTTTGCAAAGTGGGGTCCCATC GAGGTTCAGCGGCAGTGGATCTGGGACAGA TTTCACTCTCACCATCAGCAGCCTGCAGCCT GAAGATTTTGCAACTTATTACTGTCAACAG CTTAATAGTTACCCTCCGGAGGTCACCTT CGGCCAAGGGACACGACTGGAGATTAAAC SARS-CoV-S2X195-VmAb VH (aa)444 EVHLVQSGAEVKKPGASVKVSCKASGYTFT DYYMHWVRQAPGQGLEWMGWINPKIGGTN YAQKFQGRVTMTRDTSINTAYMELSRLRSDD TAVYYCAKDLQPSYCTDGVCWPDDAFDIW GQGTMVTVSS SARS-CoV-2S2X195-V1445 GYTFTDYY 222 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAb CDRH(aa)SARS-CoV-S2X195-VmAbCDRH(aa)446 INPKIGGT SARS-CoV-S2X195-VmAbCDRH(aa)447 AKDLQPSYCTDGVCWPDDAFDI SARS-CoV-S2X195-VmAb VL (aa)448 AIRMTQTPLSLSVTPGQPASISCKSSQSLLHSD GKTYLYWYLQKPGQSPQLLIYEVSNRFSGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ SIQLPPTFGQGTKVEIK SARS-CoV-S2X195-VmAbCDRL(aa)449 QSLLHSDGKTY SARS-CoV-S2X195-VmAb CDRL(aa)450 EVS SARS-CoV-S2X195-VmAb CDRL(aa)451 MQSIQLPPT SARS-CoV-S2X195-VmAb VH (nt)452 GAGGTGCATCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GACTACTATATGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAAGATTGGTGGCACAAACT ATGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACACGTCCATCAACACAGCCT ACATGGAACTGAGCAGGCTGAGATCTGACG ACACGGCCGTATATTACTGTGCGAAAGATC TACAACCTTCATATTGTACTGATGGTGTA TGCTGGCCCGATGATGCTTTTGATATCTG GGGCCAAGGGACAATGGTCACCGTCTCTTC AG 223 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2X195-VmAb VL (nt)453 GCCATCCGGATGACCCAGACTCCACTCTCT CTGTCCGTCACCCCTGGACAGCCGGCCTCC ATCTCCTGCAAGTCTAGTCAGAGCCTCCTG CATAGTGATGGAAAGACCTATTTGTATTG GTACCTGCAGAAGCCAGGCCAGTCTCCACA GCTCCTGATCTATGAAGTTTCCAACCGCTT CTCTGGAGTGCCAGATAGGTTCAGTGGCAG CGGGTCAGGGACAGATTTCACACTGAAAAT CAGCCGGGTGGAGGCTGAGGATGTTGGGGT TTATTACTGCATGCAAAGTATACAGCTTC CTCCGACGTTCGGCCAAGGGACCAAGGTG GAAATCAAAC SARS-CoV-S2X219-VmAb VH (aa)454 EVQLVESGGGLIQPGGSLRLSCAATGITVSRN YINWVRQAPGKGLEWVSIIYSGGTTYYADSV KGRFTISRDNSKNTLYLQMNSLRAEDTAVYY C ARDEN VVGGFDIWGQGTMVTVS S SARS-CoV-S2X219-VmAbCDRHl(aa)455 GITVSRNY SARS-CoV-S2X219-VmAbCDRH(aa)456 IYSGGTT SARS-CoV-S2X219-VmAbCDRH(aa)457 ARDLNVVGGFDI SARS-CoV-S2X219-VmAb VL (aa)458 EIVMTQSPGTLSLSPGERATLSCRASQSISSSY LAWYQQKPGQAPRLLIYGASSRATGIPDRFSG SGSGTDFTLTISRLEPEDFAVYYCQQYGSSPPI TFGQGTRLEIK SARS-CoV-S2X219-VmAb CDRL(aa)459 QSISSSY SARS-CoV-S2X219-VmAb CDRL2460 GAS 224 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence (aa)SARS-CoV-S2X219-VmAb CDRL(aa)461 QQYGSSPPIT SARS-CoV-S2X219-VmAb VH (nt)462 GAGGTGCAGCTGGTGGAGTCTGGAGGAGG CTTGATCCAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGCCACTGGGATCACCGTCA GTAGGAACTACATAAACTGGGTCCGCCAG GCTCCAGGGAAGGGGCTGGAGTGGGTCTCA ATTATTTATAGCGGTGGTACTACATACTA CGCAGACTCCGTGAAGGGCCGATTCACCAT CTCCAGAGACAATTCCAAGAACACGCTGTA TCTTCAAATGAACAGCCTGAGAGCCGAGGA CACGGCCGTGTATTACTGTGCGAGAGATTT AAACGTAGTGGGTGGTTTTGATATCTGGG GCCAAGGGACAATGGTCACCGTCTCTTCAG SARS-CoV-S2X219-VmAb VL (nt)463 GAAATAGTGATGACGCAGTCTCCAGGCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTATTAGC AGCAGCTACTTAGCCTGGTACCAGCAGAA ACCTGGCCAGGCTCCCAGGCTCCTCATCTA TGGTGCATCCAGCAGGGCCACTGGCATCC CAGACAGGTTCAGTGGCAGTGGGTCTGGGA CAGACTTCACTCTCACCATCAGCAGACTGG AGCCTGAAGATTTTGCAGTGTATTACTGTC AGCAGTATGGTAGCTCACCTCCGATCAC CTTCGGCCAAGGGACACGACTGGAGATTAA AC SARS-CoV-S2X244-VmAb VH (aa)464 QVQLVQSGTEVKKPGSSVKVSCKASGGTFST FSINWVRQAPGQGLEWMGRIIPILKIADYAQ KFQGRVTITADKSTSTAYMELSSLRSEDTAVY Y C ARE AGFFEE Y GSGSSGLDPWGQGTL VT V SS SARS-CoV-S2X244-VmAbCDRH(aa)465 GGTFSTFS SARS-CoV-2S2X244-V1466 IIPILKIA 225 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAhCDRH(aa)SARS-CoV-S2X244-VmAhCDRH(aa)467 AREAGFFEEYGSGSSGLDP SARS-CoV-S2X244-VmAh VL (aa)468 EIVLTQSPLSLPVTPGEPASISCRSNLSLLHSN GYNYLDWYLQKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFILKISRVEAEDVGVYYCMQ ALQTPRFGGGTKVEIK SARS-CoV-S2X244-VmAhCDRL(aa)469 LSLLHSNGYNY SARS-CoV-S2X244-VmAh CDRL(aa)470 LGS SARS-CoV-S2X244-VmAh CDRL(aa)471 MQALQTPR SARS-CoV-S2X244-VmAh VH (nt)472 CAGGTCCAGCTGGTGCAATCTGGGACTGAG GTGAAGAAGCCTGGGTCCTCGGTGAAGGTC TCCTGCAAGGCTTCTGGAGGCACCTTCAG CACCTTTAGTATCAACTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAA GGATCATCCCTATCCTTAAAATAGCAGAC TACGCACAGAAGTTCCAGGGCAGAGTCACG ATTACCGCGGACAAATCCACGAGCACAGCC TACATGGAGCTGAGCAGCCTGAGATCTGAG GACACGGCCGTGTATTACTGTGCGAGAGA GGCAGGCTTCTTCGAGGAGTATGGTTCG GGGAGTTCAGGGCTCGACCCCTGGGGCC AGGGAACCCTGGTCACCGTCTCCTCAG SARS-CoV-S2X244-VmAh VL (nt)473 GAAATTGTGTTGACACAGTCTCCACTCTCCC TGCCCGTCACCCCTGGAGAGCCGGCCTCCA TCTCCTGCAGGTCTAATCTGAGTCTCCTGC ATAGTAATGGATACAACTATTTGGATTGG TACCTGCAGAAGCCAGGGCAGTCTCCACAG CTCCTGATCTATTTGGGTTCTAATCGGGCC 226 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence TCCGGGGTCCCTGACAGGTTCAGTGGCAGT GGATCAGGCACAGATTTTATACTGAAAATC AGCAGAGTGGAGGCTGAGGATGTTGGGGTT TATTACTGCATGCAAGCTCTCCAAACTCC TCGTTTCGGCGGAGGGACCAAGGTGGAGA TCAAAC SARS-CoV-S2X246-VmAb VH (aa)474 QVQLVESGGGLVQPGGSLRLSCAASGFTFSI YEMNWVRQAPGKGLEWVSYITSGGHTIFYA DSVKGRFTISRDNAKNSLYLQMNSLRAEDTA VYYCASNPSSGYFFDYWGQGTLVTVSS SARS-CoV-S2X246-VmAbCDRH(aa)475 GFTFSIYE SARS-CoV-S2X246-VmAbCDRH(aa)476 ITSGGHTI SARS-CoV-S2X246-VmAbCDRH(aa)477 ASNPSSGYFFDY SARS-CoV-S2X246-VmAb VL (aa)478 AIRMTQSPSTLSASVGDRVTITCRASQSISSWL AWYQQKPGKAPKLLIYKASSLESGVPSRFSGS GSGTEFTLTISSLQPDDFATYYCQQYNSYPYT FGQGTKLDIK SARS-CoV-S2X246-VmAbCDRL(aa)479 QSISSW SARS-CoV-S2X246-VmAbCDRL(aa)480 KAS SARS-CoV-S2X246-VmAb CDRL(aa)481 QQYNSYPYT SARS-CoV-2482 CAGGTACAGCTGGTGGAGTCTGGGGGAGGC 227 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2X246-VmAb VH (nt)TTGGTCCAGCCTGGAGGGTCCCTGAGGCTC TCCTGTGCAGCCTCTGGATTCACCTTCAGT ATTTATGAAATGAACTGGGTCCGCCAGGCT CCAGGGAAGGGGCTGGAGTGGGTTTCATAC ATTACTAGTGGTGGTCATACCATATTCTA CGCAGACTCTGTGAAGGGCCGATTCACCAT CTCCAGAGACAACGCCAAGAACTCACTGTA TCTGCAAATGAACAGCCTGCGAGCCGAGGA CACGGCTGTTTATTACTGTGCGAGCAACCC GAGTAGTGGTTATTTTTTTGACTACTGGG GCCAGGGAACCCTGGTCACCGTCTCCTCAG SARS-CoV-S2X246-VmAb VL (nt)483 GCCATCCGGATGACCCAGTCTCCTTCCACC CTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGCCGGGCCAGTCAGAGTATTAGT AGCTGGTTGGCCTGGTATCAGCAGAAACC AGGGAAAGCCCCTAAGCTCCTGATCTATAA GGCGTCTAGTTTAGAAAGTGGGGTCCCATC AAGGTTCAGCGGCAGTGGATCTGGGACAGA ATTCACTCTCACCATCAGCAGCCTGCAGCC TGATGATTTTGCAACTTATTACTGCCAACA GTATAATAGTTATCCCTACACTTTTGGCC AGGGGACCAAGCTGGACATCAAAC SARS-CoV-S2X256-VmAb VH (aa)484 EVQLVESGGGLIQPGGSLRLSCAASGIIVSSNYMNWVRQAPGRGLEWVSVTYSGGSTFYADSVKGRFTISRDSSKNTLYLQMNGLRAEDTAVYYCARDLGESGMDVWGQGTTVTVSS SARS-CoV-S2X256-VmAbCDRH(aa)485 GIIVSSNY SARS-CoV-S2X256-VmAbCDRH(aa)486 IYSGGST SARS-CoV-S2X256-VmAbCDRH(aa)487 ARDLGESGMDV SARS-CoV-2S2X256-V1488AIRMTQSPSSVSASVGDRVTITCRASQGISSW LAWYQQKPGKAPKLLISAASNLQSGVPSRFS 228 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence mAb VL (aa) GSGSGTDFTLTISSLQPEDFATYYCQQANSFP RFGPGTKVDIK SARS-CoV-S2X256-VmAbCDRL(aa)489 QGISSW SARS-CoV-S2X256-VmAbCDRL(aa)490 AAS SARS-CoV-S2X256-VmAb CDRL(aa)491 QQANSFPR SARS-CoV-S2X256-VmAb VH (nt)492 GAGGTGCAGCTGGTGGAGTCTGGAGGGGG CTTGATCCAGCCTGGGGGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGGATCATCGTCAG TAGCAACTACATGAACTGGGTCCGCCAGG CTCCAGGGAGGGGGCTGGAGTGGGTCTCAG TTATTTATAGCGGTGGTAGCACATTCTAC GCAGACTCCGTGAAGGGCCGATTCACCATC TCCAGAGACAGTTCCAAGAACACACTGTAT CTTCAAATGAACGGCCTGAGAGCCGAGGAC ACGGCCGTGTATTACTGTGCGAGAGATCT GGGAGAGAGCGGTATGGACGTCTGGGGC CAAGGGACCACGGTCACCGTCTCCTCA SARS-CoV-S2X256-VmAb VL (nt)493 GCCATCCGGATGACCCAGTCTCCATCTTCC GTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGTCGGGCGAGTCAGGGTATTAG CAGCTGGTTAGCCTGGTATCAGCAGAAAC CAGGGAAAGCCCCTAAGCTCCTGATCTCTG CTGCATCCAATTTGCAAAGTGGGGTCCCAT CTAGGTTCAGCGGCAGTGGATCTGGGACAG ATTTCACTCTCACCATCAGCAGCCTGCAGC CTGAAGATTTTGCAACTTACTATTGTCAAC AGGCTAACAGTTTCCCTCGGTTCGGCCCT GGGACCAAAGTGGATATCAAAC SARS-CoV-S2X269-VmAb VH (aa)494EVQLVESGGGVVQPGRSLRLSCAASGFAFRI YGMHWVRQAPGKGLEWVALIWFDGNNKY YAD SVKGRFTISRDS SKNTLFLQMNSLRAEDT 229 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ALYYCARAMCPGETGNLLGICYAPDYWGQ GTLVTVSS SARS-CoV-S2X269-VmAb CDRH(aa)495 GFAFRTYG SARS-CoV-S2X269-VmAbCDRH(aa)496 IWFDGNNK SARS-CoV-S2X269-VmAbCDRH(aa)497 ARAMCPGETGNLLGICYAPDY SARS-CoV-S2X269-VmAb VL (aa)498 QSALTQPPSASGTPGQRVTISCSGSTSNIGSNH VCWYQQLPGTAPKLLIYSNNQRPSGVPDRFS GSKSGTSASLAISGLRSEDEADYYCAAWDDR LSGWVFGGGTKLTVL SARS-CoV-S2X269-VmAbCDRL(aa)499 TSNIGSNH SARS-CoV-S2X269-VmAb CDRL(aa)500 SNN SARS-CoV-S2X269-VmAb CDRL(aa)501 AAWDDRLSGWV SARS-CoV-S2X269-VmAb VH (nt)502 GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CGTGGTCCAGCCTGGGAGGTCCCTGAGACT CTCCTGTGCAGCGTCTGGATTCGCCTTCAG AATCTATGGCATGCACTGGGTCCGCCAGG CTCCAGGCAAGGGGCTGGAGTGGGTGGCAC TTATATGGTTTGATGGAAATAATAAATAC TATGCCGACTCCGTGAAGGGCCGATTCACC ATCTCCAGAGACAGTTCCAAGAACACACTG TTTCTGCAAATGAACAGCCTGAGAGCCGAG GACACGGCTCTGTATTACTGTGCGAGAGC 230 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GATGTGCCCAGGCGAAACGGGAAATCTT CTGGGTATCTGCTACGCCCCTGACTACTG GGGCCAGGGAACCCTGGTCACCGTCTCCTC AG SARS-CoV-S2X269-VmAb VL (nt)503 CAGTCTGCCCTGACTCAGCCACCCTCAGCG TCTGGGACCCCCGGGCAGAGGGTCACCATC TCTTGTTCTGGAAGCACCTCCAACATCGGA AGTAATCATGTATGCTGGTACCAGCAGCTC CCAGGAACGGCCCCCAAACTCCTCATCTAT AGTAATAATCAACGGCCCTCAGGGGTCCCT GACCGATTCTCTGGCTCCAAGTCTGGCACC TCAGCCTCCCTGGCCATCAGTGGGCTCCGG TCCGAGGATGAGGCTGATTATTACTGTGCA GCATGGGATGACAGACTGAGTGGCTGGG TGTTCGGCGGAGGGACCAAGCTGACCGTCC TAG SARS-CoV-S2X278-vl mAb VH (aa)504 QVRLQESGPGLVKPSETLSLTCAVSGYSISSG YYWGWIRQPPGKGLEWIGSTYESGSTYNNPS LKSRVTMSVDTSKNQFSLKLTSVTAADTAVY YC ARDLWEGD YYRVGDYWGQGTL VT VS S SARS-CoV-S2X278-vmAbCDRH(aa)505 GYSISSGYY SARS-CoV-S2X278-vmAbCDRH(aa)506 IYESGST SARS-CoV-S2X278-vmAbCDRH(aa)507 ARDLWEGDYYRVGDY SARS-CoV-S2X278-vmAb VL (aa)508 QSVLTQPPSVSAAPGQKVTISCSGSSSNVGNN YVSWYQQLPGTAPKLLIYDNTKRPSWIPDRF S GSKSGTSATLDITGLQTGDEADYYCGTWDSS LSVVFGGGTKLTVL SARS-CoV-S2X278-vmAb CDRL1509 SSNVGNNY 231 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence (aa)SARS-CoV-S2X278-vl mAb CDRL(aa)510 DNT SARS-CoV-S2X278-vmAb CDRL(aa)511 GTWDSSLSVV SARS-CoV-S2X278-vmAb VH (nt)512 CAGGTGCGGCTGCAGGAGTCGGGCCCAGG ACTGGTGAAGCCTTCGGAGACCCTGTCCCT CACCTGCGCTGTCTCTGGTTACTCCATCAG CAGTGGTTACTACTGGGGCTGGATCCGGC AGCCCCCAGGGAAGGGGCTGGAGTGGATT GGGAGTATCTATGAGAGTGGGAGCACCT ACAACAACCCGTCCCTCAAGAGTCGAGTCA CCATGTCAGTAGACACGTCCAAGAACCAGT TCTCCCTGAAGCTGACCTCTGTGACCGCCG CAGACACGGCCGTATATTACTGTGCGAGA GACCTCTGGGAAGGTGACTACTACCGAG TGGGGGACTACTGGGGCCAGGGAACCCTG GTCACCGTCTCCTCAG SARS-CoV-S2X278-vmAb VL (nt)513 CAGTCTGTGTTGACGCAGCCGCCCTCAGTG TCTGCGGCCCCAGGACAGAAGGTCACCATC TCCTGCTCTGGAAGCAGCTCCAACGTTGG GAATAATTATGTATCCTGGTACCAGCAGCT CCCAGGAACAGCCCCCAAACTCCTCATTTA TGACAATACTAAGCGACCCTCATGGATTCC TGACCGATTCTCTGGCTCCAAGTCTGGCAC GTCAGCCACCCTGGACATCACCGGACTCCA GACTGGGGACGAGGCCGATTATTACTGCGG AACATGGGATAGCAGTCTGAGTGTTGTC TTCGGCGGAGGGACCAAGCTGACCGTCCTA G SARS-CoV-S2M7-V1 mAb VH(aa)514QVQLVQSGAEVKKPGASVKVSCKASGYTFT SYYMHWVRQAPGQGLEWMGIINPSGTSTSY AQKFQGRVTMTRDTSTSTVYLDLSSLRSGDT AVYYC ATGSWELPS YFD YWGQGTLIT VS SSARS-CoV-2S2M7-V1 mAb CDRH1 (aa)515 GYTFTSYY 232 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-2S2M7-V1 mAb CDRH2 (aa)516 INPSGTST SARS-CoV-2S2M7-V1 mAb CDRH3 (aa)517 ATGSWELPSYFDY SARS-CoV-2S2M7-V1 mAb VL (aa)518 DIVMMQSPGTLSLSPGERATLSCRASQSTYSS YL AWYQQKPGQ APRLLISGAS SRATGIPDRF S GSGSGTDFTLTISRLEPADFAVYYCQQYGSSP PTFGQGTKVEIK SARS-CoV-2S2M7-V1 mAbCDRL1 (aa)519 QSTYSSY SARS-CoV-2S2M7-V1 mAb CDRL2 (aa)520 GAS SARS-CoV-2S2M7-V1 mAbCDRL3 (aa)521 QQYGSSPPT SARS-CoV-S2M7-V1 mAb VH(nt)522 CAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTT TCCTGCAAGGCATCTGGATACACCTTCAC CAGCTACTATATGCACTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAA TAATCAACCCTAGTGGTACTAGCACAAGC TACGCACAGAAGTTCCAGGGCAGAGTCACT ATGACCAGGGACACGTCCACGAGCACAGTC TACTTGGACCTGAGCAGCCTGAGATCTGGT GACACGGCCGTGTATTACTGTGCGACCGG GTCGTGGGAGCTACCTTCCTACTTTGACT ACTGGGGCCAGGGAACCCTGATCACCGTCT CCTCAG SARS-CoV-2S2M7-V1 mAb VL(nt)523 GACATCGTGATGATGCAGTCTCCAGGCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTATTTAC AGCAGCTATTTAGCCTGGTACCAGCAGAA ACCTGGCCAGGCTCCCAGGCTCCTCATCTCT GGTGCATCCAGCAGGGCCACTGGCATCCC AGACAGGTTCAGTGGCAGTGGGTCTGGGAC AGACTTCACTCTCACCATCAGCAGACTGGA 233 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GCCTGCGGATTTTGCAGTGTATTACTGTCA GCAGTATGGTAGCTCACCTCCGACGTTCG GCCAAGGGACCAAGGTGGAAATCAAAC SARS-CoV-2S2M11-VmAb VH (aa)524 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYMHWVRQAPGQGLEWMGWINPISSGTS YAQTFQGRVTMTSDTSITTAYMELSRLRSDD TAVYYCARAAPFYDFWSGYSYFDYWGQGT LVTVSS SARS-CoV-S2Mll-vl mAbCDRH(aa)525 GYTFTGYY SARS-CoV-S2Mll-vl mAbCDRH(aa)526 INPISSGT SARS-CoV-S2Mll-vl mAbCDRH(aa)527 ARAAPFYDFWSGYSYFDY SARS-CoV-2S2Mll-vl mAb VL (aa)528 EIVMMQSPGTLSLSPGERATLSCRASQSVSSS YLAWYQQKPGQAPRLLIYGASSRATGIPDRFS GSGSGTDFTLTISRLEPEDFAVYYCQQYGSSA WTFGQGTKVEIK SARS-CoV-S2Mll-vl mAbCDRL(aa)529 QSVSSSY SARS-CoV-S2Mll-vl mAbCDRL(aa)530 GAS SARS-CoV-S2Mll-vl mAb CDRL(aa)531 QQYGSSAWT SARS-CoV-2S2Mll-vl mAb VH (nt)532GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC 234 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GGCTACTATATGCACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTATCAGTAGTGGCACAAGCT ATGCACAGACATTTCAGGGCAGGGTCACCA TGACCAGTGACACGTCCATCACCACAGCCT ACATGGAGCTCAGCAGGCTGAGATCTGACG ACACGGCCGTATATTACTGTGCGAGAGCA GCCCCGTTTTACGATTTTTGGAGTGGTTA TTCTTACTTTGACTACTGGGGCCAGGGAA CCCTGGTCACCGTCTCCTCAG SARS-CoV-S2M11-VmAb VL (nt)533 GAAATAGTGATGATGCAGTCTCCAGGCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTGTTAG CAGCAGCTACTTAGCCTGGTACCAGCAGA AACCTGGCCAGGCTCCCAGGCTCCTCATCT ATGGTGCATCCAGCAGGGCCACTGGCATC CCAGACAGGTTCAGTGGCAGTGGGTCTGGG ACAGACTTCACTCTCACCATCAGCAGACTG GAGCCTGAAGATTTTGCAGTGTATTACTGT CAGCAGTATGGTAGCTCAGCGTGGACGT TCGGCCAAGGGACCAAGGTGGAAATCAAA C SARS-CoV-2S2M16-VmAb VH (aa)534 EVQLVESGGGMVLPGRSLRLSCAASGFTFSNYAMHWVRQAPGKGLEWVAVISYDGSTKYF ADSVKGRFTISRDTSKNTLYLQMNSLRAEDT AVYYCAKDSETCSSFTCYCDYWGRGTLVTV ss SARS-CoV-S2M16-VmAbCDRH(aa)535 GFTFSNYA SARS-CoV-S2M16-VmAbCDRH(aa)536 ISYDGSTK SARS-CoV-S2M16-VmAbCDRH(aa)537 AKDSETCSSFTCYCDY SARS-CoV-2538 DIVLTQSPDSLAVSLGERATINCKSSQSVLYSS 235 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2M16-VmAh VL (aa)NNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTIGSLQAEDVAVYYCQQYYNTPFTFGPGTKVDIK SARS-CoV-S2M16-VmAhCDRL(aa)539 QSVLYSSNNKNY SARS-CoV-S2M16-VmAh CDRL(aa)540 WAS SARS-CoV-S2M16-VmAh CDRL(aa)541 QQYYNTPFT SARS-CoV-S2M16-VmAh VH (nt)542 GAGGTGCAACTGGTGGAGTCTGGGGGAGG CATGGTCCTGCCTGGGAGGTCCCTGAGACT CTCCTGTGCAGCCTCTGGATTCACCTTCAG TAACTATGCCATGCACTGGGTCCGCCAGGC TCCAGGCAAGGGGCTGGAGTGGGTGGCAGT TATATCGTATGATGGAAGTACTAAATACT TTGCAGACTCCGTGAAGGGCCGATTCACCA TCTCCAGAGACACTTCCAAGAACACGCTGT ATCTGCAAATGAACAGCCTGAGAGCTGAGG ACACGGCTGTGTATTACTGTGCGAAAGATT CAGAGACTTGTAGTAGTTTCACCTGCTAT TGCGACTACTGGGGCCGGGGAACCCTGGT CACCGTCTCCTCAG SARS-CoV-S2M16-VmAh VL (nt)543 GATATTGTGCTGACTCAGTCTCCAGACTCCC TGGCTGTGTCTCTGGGCGAGAGGGCCACCA TCAACTGCAAGTCCAGCCAGAGTGTTTTAT ACAGCTCCAACAATAAGAACTACTTAGCT TGGTACCAGCAGAAACCAGGACAGCCTCCT AAGCTGCTCATTTACTGGGCATCTACCCGG GAATCCGGGGTCCCTGACCGATTCAGTGGC AGCGGGTCTGGGACAGATTTCACTCTCACC ATCGGCAGCCTGCAGGCTGAAGATGTGGCA GTTTATTACTGTCAGCAATATTATAATACT CCCTTCACTTTCGGCCCTGGGACCAAAGTG GATATCAAAC 236 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-2S2M28-VmAb VH (aa)544EVQLVESGGGVVQPGRSLRLSCAASGFTFSS YGMHWVRQAPGKGLEWVTVIWYDGSNRY YADSVKGRFTISRDNSKNTLYLQMDSLRAED TAVYYCARAVAGEWYFDYWGQGTLVTVSS SARS-CoV-S2M28-VmAbCDRH(aa)545 GFTFSSYG SARS-CoV-S2M28-VmAbCDRH(aa)546 IWYDGSNR SARS-CoV-S2M28-VmAbCDRH(aa)547 ARAVAGEWYFDY SARS-CoV-S2M28-VmAb VL (aa)548SYELTQPPSVSVSPGQTARITCSGDALAKHYA YWYRQKPGQAPVLVIYKDSERPSGIPERFSGS SSGTTVTLTISGVQAEDEADYYCQSADSIGSS WVFGGGTKLTVLSARS-CoV-S2M28-VmAbCDRL(aa)549 ALAKHY SARS-CoV-S2M28-VmAbCDRL(aa)550 KDS SARS-CoV-S2M28-VmAb CDRL(aa)551 QSADSIGSSWV SARS-CoV-S2M28-VmAb VH (nt)552 GAGGTGCAGCTGGTGGAGTCTGGGGGAGG CGTGGTCCAGCCTGGGAGGTCCCTGAGACT CTCCTGTGCAGCGTCTGGATTCACCTTCAG TAGCTATGGCATGCACTGGGTCCGCCAGG CTCCAGGCAAGGGGCTGGAGTGGGTGACA GTTATTTGGTATGATGGAAGTAATCGATA CTATGCAGACTCCGTGAAGGGCCGATTCAC CATCTCCAGAGACAATTCCAAGAACACGCT GTATCTGCAAATGGACAGCCTGAGAGCCGA 237 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GGACACGGCTGTTTATTACTGTGCGAGAGC AGTGGCCGGGGAATGGTACTTTGACTAC TGGGGCCAGGGAACCCTGGTCACCGTCTCC TCAG SARS-CoV-S2M28-VmAb VL (nt)553 TCCTATGAGCTGACACAGCCACCCTCGGTG TCAGTGTCCCCAGGACAGACGGCCAGGATC ACCTGCTCCGGAGATGCATTGGCAAAACA CTATGCTTATTGGTACCGGCAGAAGCCAGG CCAGGCCCCTGTGCTGGTGATATATAAAGA CAGTGAGAGGCCCTCAGGGATCCCTGAGC GATTCTCTGGCTCCAGCTCAGGGACAACAG TCACGTTGACCATCAGTGGAGTCCAGGCAG AAGACGAGGCTGACTATTACTGTCAATCAG CAGACAGCATTGGTAGTTCTTGGGTGTTC GGCGGAGGGACCAAGCTGACCGTCCTAG SARS-CoV-2S2L49-VmAb VH (aa)554 EVQLLESGGGVVQPGGSLRLSCAASGFNFSS YGMHWVRQAPGKGLEWVAFMRYDETNKY YADSVKGRFTISRDNSKNTLYLQMNSLRAED TAVYYCAKDRLLGGPYYDSIGLDYWGQGT LVTVSSSARS-CoV-S2L49-VmAbCDRH(aa)555 GFNFSSYG SARS-CoV-S2L49-VmAbCDRH(aa)556 MRYDETNK SARS-CoV-S2L49-VmAbCDRH(aa)557 AKDRLLGGPYYDSIGLDY SARS-CoV-S2L49-VmAb VL (aa)558 DIVMTQSPSSVSASVGDRVTITCRASQGISSW LAWYQQKPGKAPKLLIYTASSLQSGVPSRFS GSESGTDFTLTISSLQPEDFATYYCQQGYSFP YTFGQGTKLEIK SARS-CoV-S2L49-VmAb CDRL(aa)559 QGISSW SARS-CoV-2S2L49-V1560 TAS 238 WO 2021/226560 PCT/US2021/031442 Sequence DescriptionSEQ ID NO.Sequence mAb CDRL2(aa)SARS-CoV-S2L49-VmAb CDRL(aa)561 QQGYSFPYT SARS-CoV-2S2L49-VmAb VH (nt)562 GAGGTGCAGCTGTTGGAGTCTGGGGGAGGC GTGGTCCAGCCTGGGGGGTCCCTGAGACTC TCCTGTGCAGCGTCTGGATTCAACTTCAGT AGCTATGGCATGCACTGGGTCCGCCAGGC TCCAGGCAAGGGGCTGGAGTGGGTGGCATT TATGCGGTATGATGAAACTAATAAATACT ATGCAGACTCCGTGAAGGGCCGATTCACCA TCTCCAGAGACAATTCCAAGAACACGCTGT ATCTGCAAATGAACAGCCTGAGAGCTGAGG ACACGGCTGTGTATTACTGTGCGAAAGATC GGCTATTAGGAGGTCCATATTATGATAG CATTGGGCTTGACTACTGGGGCCAGGGAA CCCTGGTCACCGTCTCCTCAG SARS-CoV-2S2L49-VmAb VL (nt)563 GATATTGTGATGACTCAGTCTCCATCTTCCG TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGTCGGGCGAGTCAGGGTATTAGC AGCTGGTTAGCCTGGTATCAGCAGAAACC AGGTAAAGCCCCTAAGCTCTTGATCTATAC TGCATCCAGTTTGCAAAGTGGGGTCCCATC AAGGTTCAGCGGCAGTGAATCTGGGACAGA TTTCACTCTCACCATCAGCAGCCTGCAGCCT GAAGATTTTGCAACTTACTATTGTCAACAG GGTTACAGTTTCCCGTACACTTTTGGCCA GGGGACCAAGCTGGAGATCAAAC SARS-CoV-2S2D65-VmAb VH (aa)564 EVQLVESGGGLAQPGRSLRLSCAVSGFSFDD YAMHWVRQVPGKGLEWVSGISWNSGNIGY ADSAKGIH'HSWNAKNSLYLQMNSLRPEDT AL Y Y C VKD KHYDSS GY V V N GF DY W GQ GIL VTVSP SARS-CoV-S2D65-VmAbCDRH(aa)565 GFSFDDYA SARS-CoV-2566 ISWNSGNI 239 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2D65-VmAb CDRH(aa)SARS-CoV-S2D65-VmAbCDRH(aa)567 VKDKHYDSSGYVVNGFDY SARS-CoV-S2D65-VmAb VL (aa)568 DIQLTQSPSFLSASVGDRVTITCRASQDISSYL AWYQQKPGKAPKLLIYAASTLQSGVPSRFSG SGSGTEFTLTIRSLQPEDFATYYCQQLHSYPA TFGQGTKVEIK SARS-CoV-S2D65-VmAbCDRL(aa)569 QDISSY SARS-CoV-S2D65-VmAbCDRL(aa)570 AAS SARS-CoV-S2D65-VmAb CDRL(aa)571 QQLHSYPAT SARS-CoV-S2D65-VmAb VH (nt)572 GAGGTGCAGCTGGTGGAGTCGGGGGGAGG CTTGGCACAGCCTGGCAGGTCCCTGAGACT CTCCTGTGCAGTCTCTGGATTCAGCTTTGA TGATTATGCCATGCACTGGGTCCGGCAAGT TCCAGGGAAGGGCCTGGAGTGGGTCTCAGG TATTAGTTGGAATAGTGGTAACATAGGCT ATGCGGACTCTGCGAAGGGCCGATTCACCA TCTCCAGAGACAACGCCAAGAACTCCCTGT ATCTGCAAATGAACAGTCTGAGACCTGAGG AC AC GGCCT1 G ؛'VF i'ACl GFGl'AAAAGATA AACATTATGATAGTAGTGGTTACGTAGTA AATGGATTTGACTACTGGGGCCAGGGAAC CCTGGTCACCGTCTCCCCAG SARS-CoV-S2D65-VmAb VL (nt)573GACAlXX:AGIlX3ACCCAG ׳rCl'CCA r rCCI' r rCC TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGCCGGGCCAGTCAGGACATTAGC AGTTATTTGGCCTGGTATCAGCAAAAACCA 240 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GGGAAAGCCCCTAAGCTCCTGATCTATGCT GCATCCACTTTGCAAAGTGGGGTCCCATC/X AGGTTCAGCGGCAGTGGATCTGGGACAGAA TTCACTCTCACAATCCGCAGCCTGCAGCCT GAAGATTTTGCAACTTATTACTGTCAACAG CTTCACAGTTACCCTGCGACGTTCGGCCA AGGGACCAAGGTGGAAATCAAAC SARS-CoV-2S2D97-VmAb VH (aa)574 QITLKESGPTLVKPTQTLTLTCSFSGFSLTTSG VGVAWIRQPPGKALEWLALIYWDDDKRYSP TLRSRLTITKDTSKNQWLTMTNMDPVDTAT YYCALHQIMTVFDLWGRGALVTVSS SARS-CoV-S2D97-VmAbCDRH(aa)575 GFSLTTSGVG SARS-CoV-S2D97-VmAbCDRH(aa)576 IYWDDDK SARS-CoV-S2D97-VmAbCDRH(aa)577 ALHQIMTVFDL SARS-CoV-2S2D97-VmAb VL (aa)578 QSVLTQPASVSGSPGQSITISCTGTSSDVGDYN YVSWYQIIHPGKAPKLMIYQVTNRPSGVSNR FSGSKSGNTASLTISGLQAEDEADYYCTSYTT S S FL VE F GG GIK LI VL SARS-CoV-S2D97-VmAbCDRL(aa)579 SSDVGDYNY SARS-CoV-S2D97-VmAbCDRL(aa)580 QVT SARS-CoV-2S2D97-VmAb CDRL3581 TSYTTSSTLVE 241 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence (aa) SARS-CoV-S2D97-VmAb VH (nt)582 CAGATCACCTTGAAGGAGTCTGGTCCTACC CTGGTGAAACCCACACAGACCCTCACGCTG ACCTGCAGTTTCTCTGGGTTCTCACTCACC ACTAGTGGAGTGGGTGTGGCCTGGATCCG TCAGCCCCCAGGAAAGGCCCTGGAGTGGCT TGCACTCATTTATTGGGATGATGACAAGC GCTACAGCCCAACTCTGAGGAGCAGGCTCA CCATCACCAAGGACACCTCCAAGAACCAGG TGGTCCTTACAATGACCAACATGGACCCTG TGGACACAGCCACATATTACTGTGCACTAC ACCAGATTATGACGGTCTTCGATCTCTGG GGCCGTGGCGCCCTGGTCACTGTCTCCTCA G SARS-CoV-S2D97-VmAb VL (nt)583 CAGTCTGTGCTGACTCAGCCTGCCTCCGTGT CTGGGTCTCCTGGCCAGTCGATCACCATCTC CTGCACTGGAACCAGCAGTGACGTTGGTG ATTATAACTATGTGTCCTGGTACCAACACC ACCCAGGCAAAGCCCCCAAACTCATGATTT ATCAGGTCACTAATCGGCCCTCAGGGGTTT CTAATCXKHIVTCTGGCTCCAAGTCTGGCAA CACGGCCTCCCTGACCATCTCTGGGCTCCA GGCTGAGGACGAGGCTGATTATTACTGCAC TTCCTATACAACCAGTAGTACTCTCGTGG AATTCGGCGGAGGGACCAAGTTGACCGTCT TAG SARS-CoV-S2D106-VmAb VH (aa)584 EVQLVQSGAEVKKPGSSVKVSCKASGGPFSS SAISWVRQAPGQGLEWMGGIIPMVGTANYA QKFQGRVTITADESTSTAYMELSSLRSEDTAV YYCARDSRYCSGGSCYSVWFDPWGQGTLV TVSS SARS-CoV-S2D106-VmAbCDRH1 (aa)585 GGPFSSSA SARS-CoV-S2D106-VmAbCDRH(aa)586 IIPMVGTA 242 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence SARS-CoV-S2D106-VmAb CDRH(aa)587 ARDSRYCSGGSCYSVWFDP SARS-CoV-S2D106-VmAb VL (aa)588 DIQLTQSPSSLSASVGDRVTITCRASQSISSYL NWYQQKPGKAPKVLIYAASSLQSGVPSRFSG SGSGTDFTLTISSLQPEDFATYYCQQSYSTPR TFGQGTKVEMK SARS-CoV-S2D106-VmAbCDRL(aa)589 QSISSY SARS-CoV-S2D106-VmAbCDRL(aa)590 AAS SARS-CoV-S2D106-VmAb CDRL(aa)591 QQSYSTPRT SARS-CoV-S2D106-VmAb VH (nt)592 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGTCCTCGGTGAAGGTT TCCTGCAAGGCTTCTGGAGGCCCCTTCAG CAGCTCTGCTATCAGCTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAG GGATCATCCCTATGGTAGGTACAGCAAAC TATGCACAGAAGTTCCAGGGCAGAGTCACG ATTACCGCGGACGAATCCACGAGCACAGCC TACATGGAGCTGAGCAGCCI'GAGATCTGAG GACACGGCCGTGTATTACTGTGCGAGAGA TTCGCGCTATTGTAGTGGTGGTAGCTGC TACTCCGTCTGGTTCGACCCCTGGGGCCA GGGAACCCTGGTCACCGTCTCCTCAG SARS-CoV-S2D106-VmAb VL (nt)593 GACATCCAGTTGACCCAGTCTCCATCCTCCC TGTCTGCATCTGTAGGAGACAGAGTCACCA TCACTTGCCGGGCAAGTCAGAGCATTAGC AGCTATTTAAATTGGTATCAGCAGAAACCA GGGAAAGCCCCTAAGGTCCTGATCTATGCT GCATCCAGTTTGCAAAGTGGGGTCCCATCA AGGTTCAGTGGCAGTGGATCTGGGACAGAT 243 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence TTCACTCTCACCATCAGCAGTCTCCAACCTG AAGATTTTGCAACTTACTACTGTCAACAGA GTTACAGTACCCCTCGAACGTTCGGCCAA. GGGACCAAGGTGGAAATGAAAC SARS-CoV-S2X149-VmAb VH (aa)594 QVQLVQSGADVKKPGASVKVSCKASGYTFT GYFIHWVRQAPGQGLEWMGWINPISGGRNY TRKFQGRITMNRDTSITTAYMELSRLRSDDTA VYYCARDSGGMDYGSGSYFSPPRYGMDVW gqgttvtvss SARS-CoV-S2X149-VmAbCDRH(aa)595 GYTFTGYF SARS-CoV-S2X149-VmAbCDRH(aa)596 INPISGGR SARS-CoV-S2X149-VmAbCDRH(aa)597 ARDSGGMDYGSGSYFSPPRYGMDV SARS-CoV-S2X149-VmAb VL (aa)598 QSVLTQPASVSGSPGQSITISCTGASSDVGAY NFVSWYQQHPGKAPKLMIYEVSNRPSGVSNR FSGSKSGNTASLTISGLQAEDEAAYYCSSYTS SNTVVFGGGTSLTVL SARS-CoV-S2X149-VmAbCDRL(aa)599 SSDVGAYNF SARS-CoV-S2X149-VmAbCDRL(aa)600 EVS SARS-CoV-S2X149-VmAb CDRL(aa)601 SSYTSSNTVV SARS-CoV-2602 CAGGTGCAGCTGGTGCAGTCTGGGGCTGAC 244 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence S2X149-VmAb VH (nt)GTGAAGAAGCCTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGGATACACCTTCACC GGCTACTTTATACACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTATCAGTGGTGGCAGAAACT ATACACGGAAGTTTCAGGGCAGGATCACCA TGAACAGGGACACGTCCATCACCACAGCCT ACATGGAGCTGAGCAGGCTGAGAI'CI'GACG ACACGGCCGTGTATTACTGTGCGAGAGATT CGGGGGGGATGGACTATGGTTCGGGGAG TTATTTTTCCCCCCCCCGATACGGTATGG ACGTCTGGGGCCAAGGGACCACGGTCACC GTCTCCTCA SARS-CoV-S2X149-VmAb VL (nt)603 CAGTCTGTGCTGACTCAGCCTGCCTCCGTGT CTGGGTCTCCTGGACAGTCGATCACCATCT CCTGCACTGGAGCCAGCAGTGACGTTGGT GCTTATAACTTTGTCTCCTGGTACCAACAG CACCCAGGCAAAGCCCCCAAACTCATGATT TATGAGGTCAGTAATCGGCCCTCAGGGGTT IC I/A AI CGCT PC PC rGGC TGC A AG IC IGGC A ACACGGCCTCCCTGACCATCTCTGGGCTCC AGGC TGAGGACGAGGCI GC TTAT TAOI GCA GCTCATATAC AAGCAGTAACACTGTGGT ATTCGGCGGAGGGACCAGCCTGACCGTCCT AG SARS-CoV-S2X179-VmAb VH (aa)604 EVQLVESGGGLVQPGGSLRLSCAASGITVSSN YMT W VRQ AP GKGLEW V S VIY S G G S TF Y AD S VRGRFTISRDNSKNTLYLQMNSLRPDDTAW YCARDLNELGIDCWGQGTLVTVSS SARS-CoV-S2X179-VmAbCDRH(aa)605 GITVSSNY SARS-CoV-S2X179-VmAbCDRH(aa)606 IYSGGST SARS-CoV-2S2X179-V1607 ARDLNELGIDC 245 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence mAhCDRH(aa) SARS-CoV-S2X179-VmAh VL (aa)608 DIVMTQTPSSVSASVGDRVTITCRASQGISRW LAWYQQKPGKAPKLLIYAASTLQSGVPSRFS GSGSGTDFTLTIRSLQPEDYATYYCQQANSFP FF GGGTKVEIK SARS-CoV-S2X179-VmAhCDRL(aa)609 QGISRW SARS-CoV-S2X179-VmAh CDRL(aa)610 AAS SARS-CoV-S2X179-VmAh CDRL(aa)611 QQANSFPF SARS-CoV-S2X179-VmAh VH (nt)612 GAGGrGCAGCrGGrGGAGICIGGGGGAGG C TT GGT C C AGCC T GGGGGGT C C CTGAGACT CTCCTGTGCAGCCTCTGGTATCACCGTCAG TAGCAATTATATGACCTGGGTCCGCCAGGC TCCAGGGAAGGGGCTGGAATGGGTCTCAGT TATTTATAGCGGTGGCAGCACATTCTACG CAGACTCCGTGAGGGGCCGATTCACCATCT CCAGAGACAATTCCAAGAACACCCTGTATC IIC AAAT GA AC AGC C r GA GA C C TG A C G AC A CGGCTGTGTATTACTGTGCGAGAGATCTG AACGAGCTGGGGATTGACTGCTGGGGCC AGGGAACCCTGGTCACCGTCTCCTCAG SARS-CoV-S2X179-VmAh VL (nt)613 GATATTGTGATGACCCAGACTCCATCTTCC GTGTCTGCATCTGTAGGAGACAGAGTCACC ATCACTTGTCGGGCGAGTCAGGGTATTAG CCGCTGGTTAGCCTGGTATCAGCAGAAAC CAGGGAAAGCCCCTAAGCTCCTGATCTATG CTGCATCCACTTTACAAAGTGGGGTCCCAT CAAGGTTCAGCGGCAGTGGATCTGGGACAG ATTTCACTCTCACCATCAGGAGCCTGCAGC CTGAAGATTATGCAACTTACTATTGTCAAC AGGCTAACAGTTTCCCTTTTTTCGGCGGA 246 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence GGGACCAAGGTGGAGATCAAAC SARS-CoV-2S2H101-VmAb VH (aa)614 EVHLVESGGGLVQPGGSLRLSCAASGFTFIN YAMSWVRQAPGKGLEWVSAIGLSGGSTNY ADSVKGRFTISRDNSKNTLSLEMNNLRAEDT avyyctkgrggyfdpfdpwgqgtlvtvss SARS-CoV-S2H101-VmAbCDRH(aa)615 GFTFINYA SARS-CoV-S2H101-VmAbCDRH(aa)616 IGLSGGST SARS-CoV-S2H101-VmAbCDRH(aa)617 TKGRGGYFDPFDP SARS-CoV-S2H101-VmAb VL (aa)618 DIVMTQSPSSLSASVGDRVTITCRASQSISSYL NWYQQIPGKAPNLLIHAASSLQSGVPSRFSGS gsgtdftltisslqpedfatyycqqsysspptf GQGTKLEIK SARS-CoV-S2H101-VmAbCDRL(aa)619 QSISSY SARS-CoV-S2H101-VmAbCDRL(aa)620 AAS SARS-CoV-S2H101-VmAb CDRL(aa)621 QQSYSSPPT SARS-CoV-S2H101-VmAb VH (nt)622 GAGGTGCATCTGGTGGAGTCTGGGGGAGGC TTGGTACAGCCTGGGGGGTCCCTGAGACTC TCCTGTGCAGCCTCTGGATTCACCTTTATC AACTATGCCATGAGCTGGGTCCGCCAGGCT CCAGGGAAGGGGCTGGAATGGGTCTCAGCT 247 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence ATTGGTCTTAGTGGTGGTAGTACAAACTA CGCAGACTCCGTGAAGGGCCGGTTCACCAT CTCCAGAGACAACTCCAAGAACACGCTGTC TTTGGAAATGAACAACCTGCGAGCCGAGGA CACGGCCGTATATTACTGTACAAAAGGGA GGGGAGGATATTTTGACCCGTTCGACCC CTGGGGCCAGGGAACCCTGGTCACCGTCTC CTCAG SARS-CoV-S2H101-VmAb VL (nt)623 GACATCGTGATGACCCAGl'Cl'CCATCCTCC CTGTCTGCATCTGTTGGGGACAGAGTCACC ATCACTTGCCGGGCAAGTCAGAGCATTAG CAGCTATTTAAATTGGTATCAGCAGATACC AGGGAAAGCCCCTAACCTCCTGATCCATGC TGCATCCAGTTTGCAAAGTGGGGTCCCATC AAGGTTCAGTGGCAGTGGATCTGGGACAGA TTTCACTCTCACCATCAGCAGTCTGCAACCT GAAGATTTTGCAACTTACTACTGTCAGCAG AGTTACAGTTCCCCTCCCACTTTTGGCCA GGGGACCAAGCTGGAGATCAAAC Antibody 409_ll_l_vVH624 EVQLVQSGAEVKKPGSSVKVSCKASGGPFSS SAISWVRQAPGQGLEWMGGHP1VGTANYAQ KFQGRVTITADESTSTAYMELSSLRSEDTAVY YCAIWSRYCSGGSCYSVWFDPWGQGTLVT VSSAntibody 409_ll_l_vCDRH2625 IIPIVGTA Antibody 409_ll_l_vVH626 EVQLVQSGAEVKKPGSSVKVSCKASGGPFSS SAISWVRQAPGQGLEWMGGIIPMVGTANYA QKFQGRVTITADESTSTAYMELSSLRSEDTAV YYCARDSRYCSGGSCYSVFFDPWGQGI'LVT VSSAntibody 409_ll_l_vCDRH3627 ARDSRYCSGGSCYSVFFDP Antibody 409_ll_l_v4VH628 EVQLVQSGAEVKKPGSSVKVSCKASGGPFSS SAISWVRQAPGQGLEWMGGHPIVGTANYAQ KFQGRVTHADESTSTAYMELSSLRSEDTAVY YCARDSRYCSGGSCYSVFFDPWGQGTLVTV SS 248 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence [reserved] 629 [reserved] Antibody 409_ll_2_vl VH630QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWDDDKRYST SLKSRLTITKDTSKNQWLTMTNMDPVDTAT YYCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vl CDRH1631 GFSVTTSGVG Antibody 409_ll_2_vl CDRH2632 IYWDDDK Antibody 409_ll_2_vl CDRH3633 ARHTIPSIFDY Antibody 409_ll_2_vVH634QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYFDDDKRYSTS :LKSRLHTKDTSKNQVVLlArrNMDPVDTAl'Y YCARHTIPSIFDWGQGILVTVSSAntibody 409_ll_2_vCDRH2635 1YFDDDK Antibody 409_ll_2_vVH636QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYYDDDKRYSTS :LKSRLHTKDTSKNQVVLlArrNMDPVDTAl'Y YCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2637 IYYDDDK Antibody 409_ll_2_vVH638QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWEDDKRYSTS :LKSRLnTKDTSKNQVVLlArrNMDPVDTAl'Y YCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2639 lYWEDDK Antibody 409_ll_2_vVH640QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWNDDKRYST SLKSRLTITKDTSKNQWLTMTNMDPVDTAT YYCARHTIPSIFDYWGQGILVTVSSAntibody 409 11 2 v5641 IYWNDDK 249 250 Antibody 409 11 2 v ll Antibody 409_ll_2_vllVH Antibody 409_ll_2_vlCDRH2 Antibody 409_ll_2_vlVH Antibody 409_ll_2_vCDRH2 Antibody 409_ll_2_v9VH Antibody 409_ll_2_vCDRH2 Antibody 409_ll_2_v8VH Antibody 409_ll_2_vCDRH2 Antibody 409_ll_2_v7VH Antibody 409_ll_24_vCDRH2 Antibody 409_ll_2_v6VH CDRH2 Sequence Description 653 652 651 650 649 648 647 646 645 644 643 642 SEQ ID NO.

IYYNDDK qitlkesgptlvkptqtltltctfsgfsvttsg VGVGWIRQPPGKALEYLALIYYNDDKRYSTS LKSRLTITKDTSKNQVVLTMTNKdDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYYEDDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYYEDDKRYSTS LKSRLTITKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYFNDDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYFNDDKRYSTS LKSRLTITKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS lYFEDDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG vgvgwirqppgkaleylaliyfeddkrysts LKSRLTITKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS iYWDNDK QITLKESGPTLV’ KPTQTLTLTCTFSGFSVTTSG vgvgwirqppgkaleylaliywdndkryst SLKSRLTITKDTSKNQWLTMTNMDPVDTAT YYCARHTIPSIFDYWGQGn>VTVSS IYWDEDK QITLKESGPTLV’ KPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWDEDKRYSI’ S LKSRLTITKDTSKNQVVI.,TMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS Sequence W O 2021/226560 PCT/US2021/031442 W O 2021/226560 PCT/US2021/031442 Sequence QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYFDEDKRYSTS LKSRLTITKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS lYFDEDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYFDNDKRYSTS LKSRLTITKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYFDNDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYYDEDKRYSTS LKSRLTFrKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYYDEDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYYDNDKRYSTS LKSRLTFrKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYYDNDK QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWEEDKRYSTS LKSRLTFrKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYWEEDK QrrLKESGPrLVKPrQILrL ’ rCrFSGFSVIISG VGVGVTRQPPGKALEYLALIYWENDKRYSTS LKSRLTITKDTSKNQVVLTMTNKdDPVDTATY YCARHTIPSIFDYWGQGILVTVSS IYWENDK SEQ ID NO. 654 655 656 657 ID so 659 099 661 Cl663 664 665 Sequence Description CDRH2 Antibody 409_ll_2_vlVH Antibody 409_ll_2_vlCDRH2 Antibody 409_ll_2_vl3VH Antibody 409_ll_2_vlCDRH2 Antibody 409_ll_2_vlVH Antibody 409_ll_2_vlCDRH2 Antibody 409_ll_2_vl5VH Antibody 409_ll_2_vlCDRH2 Antibody 409_ll_2_vlVH Antibody 409_ll_2_vlCDRH2 Antibody 409_ll_2_vlVH Antibody 409 11 2 vl7 251 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CDRH2 Antibody 409_ll_2_vlVH666QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWNEDKRYSTS LKSRLTITKDTSKNQVVI..TMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vl8CDRH2667 IYWNEDK Antibody 409_ll_2_vlVH668QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYWNNDKRYST SLKSRLTITKDTSKNQWLTMTNMDPVDTAT YYCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vlCDRH2669 IYWNNDK Antibody 409_ll_2_vVH670QITLKESGPTLYKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYFEEDKRYSTS LKSRLTITKDTSKNQVVTTMITNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2671 IYFEEDK Antibody 409_ll_2_vVH672QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSGVGVGWIRQPPGKALEYTALIYTENDKRYSTSLKSRLTITKDTSKNQVVTTMITNMDPVDTATYYCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2673 IYFENDK Antibody 409_ll_2_vVH674QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSGVGVGWIRQPPGKALEYTALIYTNEDKRYSTSLKSRLTITKDTSKNQVVTTMITNMDPVDTATYYCARHTIPSIFDYWGQGILVTVSSS2X324_v22CDRH2675 IYFNEDK Antibody 409_ll_2_vVH676QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLAL1YFNNDKRYSTS LKSRLTITKDTSKNQWLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2677 IYFNNDK 252 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence Antibody 409_ll_2_vVH678QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSGVGVGWIRQPPGK ALEYL AL IVYEEDKRY SI S LKSRLTITKDTSKNQVVLTMTNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2679 IYYEEDK Antibody 409_ll_2_vVH680QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSGVGVGWIRQPPGKALEYLALIYYENDKRYSTSLKSRLTITKDTSKNQVVI..TMTNMDPVDTATYYCARHTIPSIFDYWGQGILVTVSSAntibody 409_ll_2_vCDRH2681 IYYENDK Antibody 409_ll_2_vVH682QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVGWIRQPPGKALEYLALIYYNEDKRYSTS LKSRLTITKDTSKNQVVTTMITNMDPVDTATY YCARHTIPSIFDYWGQGILVTVSSAntibody 409_l l_2_v6 CDRH2683 IYYNEDK Antibody 409_ll_2_vVH684QITLKESGPTLVKPTQTLTLTCTFSGFSVTTSG VGVG^TRQPPGKALEYLALIYYNNDKRYSTS LKSRLTITKDTSKNQVY7LTMr mMDPVDTATY YCARHTIPSIFDYWGQGILVTVSSS2X324_v27CDRH2685 IYYNNDK Antibody 409_l 1_2 VL686 QPVLTQPASVSGSPGQSITISCTATSSDVGNYN YVSWYQHHPGKAPKLMIYEVSNRPSGVSNRF SGSKSGNTASLTISGLQAEDEADYYCSSYTSS SLLFGGGTKLTVL Antibody 409_l1_CDRL1687 SSDVGNYNY Antibody 409_l1_CDRL2688 EVS Antibody 409_l1_CDRL3689 SSYTSSSLL Antibody690 CAGATCACCTTGAAGGAGTCTGGTCCTACG 253 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence 409_l 1_2 VH (nt)CTGGTGAAACCCACACAGACCCTCACGCTG ACCTGCACCTTCTCTGGGTTCTCAGTCACT ACTAGTGGAGTGGGTGTGGGCTGGATCCG TCAGCCCCCAGGAAAGGCCCTGGAGTACCT TGCACTCATTTATTGGGATGATGATAAGC GCTACAGTACATCTCTGAAGAGCAGGCTCA CTATCACCAAGGACACCTCCAAAAACCAGG TGGTCCTTACAATGACCAACATGGACCC'rG- TGGACACAGCCACATATTACTGTGCACGCC ATACTATACCTTCGATCTTTGACTACTGG GGCCAGGGAATCCTGGTCACCGTCTCCTCA G Antibody 409_l 1_2 VL (nt)691 CAGCCTGTGCTGACTCAGCCTGCCTCCGTGT CTGGGTCTCCTGGACAGrCGATCACCATCT CCTGCACTGCAACCAGCAGTGACGTTGGT AATTATAACTATGTCTCCTGGTACCAACAC CACCCAGGCAAAGCCX'CCAAACTCATGAIY TATGAGGTCAGTAATCGGCCCTCAGGGGTT TCTAATCGCTTCTCTGGCTCCAAGTCTGGCA ACACGGCCTCCCTGACCATCTCTGGGCTCC AGGCTGAGGACGAGGCTGATTATTATTGCA GCTCATATACAAGCAGCAGTCTACTATTC GGCGGAGGGACCAAGCTGACCGTCCTAG Antibody 409_ll_3_vl VH692 EVQLVQSGAAVKKTGASVKVSCKASEYIFTE YYIHWVRQAPGQGLEWMGWINPNSGRTHY AQKFQGRVTMTRDTSITTAYMELSSLRSDDT AVYYCACLAGEWGYCSTTSCKRGIDGMDV WGQGTTVTVSSAntibody 409_ll_3_vl CDRH1693 EYIFTEYY Antibody 409_ll_3_vl CDRH2694 INPNSGRT Antibody 409_ll_3_vl CDRH3695 ACLAGEWGYCSTTSCKRGIDGMDV Antibody 409_ll_3_vl VL696EIVLTQSPLSLPVTPGEPASISCRSSQSLLHSN GYNYLNWYI.QKPGQSPQLLIYLGSNRASGVP DRFSGSGSGTDFTLKISRVEAEDVGVYYCMQ ALQTMYTFGQG1XLEIRAntibody 409 11 3 vl697 QSLLHSNGYNY 254 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CDRL1Antibody 409_ll_3_vl CDRL2698 LGS Antibody 409_ll_3_vl CDRL3699 MQALQTMYT Antibody 409_ll_3_vl VH(nt)700 GAGGTGCAACTGGTGCAGTCTGGGGCTGCG GTGAAGAAGACTGGGGCCTCAGTGAAGGTC TCCTGCAAGGCTTCTGAATACATCTTCACC GAATACTATATACACTGGGTGCGACAGGC CCCTGGACAAGGGCTTGAGTGGATGGGATG GATCAACCCTAACAGTGGTCGCACACACT ATGCACAGAAGTTTCAGGGCAGGGTCACCA TGACCAGGGACACGTCCATCACCACAGCCT ACATGGAGCTGAGTAGCCTGAGATCTGACG ACACGGCCGTGTATTACTGTGCGTGCCTTG CGGGTGAGTGGGGATATTGTAGTACGAC CAGCTGCAAAAGAGGGATTGACGGTATG GACGTCTGGGGCCAAGGGACCACGGTCAC CGTCTCCTCA Antibody 409_ll_3_vl VL(nt)701 GAAATTGTGTTGACGCAGTCTCCACTCTCCC TGCCCGTCACCCCTGGAGAGCCGGCCTCCA TCTCCTGCAGGTCTAGTCAGAGCCTCCTGC ATAGTAATGGATACAACTATTTGAATTGG TACCTGCAGAAGCCAGGGCAGTCTCCACAA CTCCTGATCTATTTGGGTTCTAATCGGGCC TCCGGGGTCCCTGACAGGTTCAGTGGCAGT GGATCAGGCACAGATTTTACACTGAAAATC AGCAGAGTGGAGGCTGAGGATGTTGGGGTT TATTACTGCATGCAAGCTCTACAAACTAT GTACACTTTTGGCCAGGGGACCAAGCTGG AGATCAGAC S2D106-VmAb VH (nt- CO)702 GAAGTGCAACTAGTGCAAAGTGGTGCAGA AGTGAAGAAGCCTGGCTCTTCTGTGAAAGT GTCTTGCAAGGCCTCTGGCGGCCCATTTTCC AGCTCTGCCATCTCCTGGGTCAGACAGGCT CCCGGCCAAGGACTGGAGTGGATGGGCGG AATCATCCCTATGGTGGGCACCGCCAACTA CGCCCAGAAGTTCCAGGGCAGAGTGACCAT CACCGCTGATGAGTCCACCTCTACAGCCTA 255 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CATGGAACTGTCCTCCCTGAGATCCGAGGA CACCGCTGTGTACTACTGTGCTCGGGACTCT CGGTATTGCTCCGGCGGCAGCTGCTACTCC GTGTGGTTCGACCCTTGGGGCCAGGGCACA CTGGTGACCGTGTCCAGC Antibody 409_ll_l_vVH (nt-CO)703 GAAGTGCAACTAGTGCAAAGTGGTGCAGA AGTCAAGAAGCCAGGCTCCAGCGTTAAAGT GTCTTGCAAGGCCTCTGGCGGACCTTTCTCC TCCAGCGCCATCTCCTGGGTGCGGCAGGCT CCTGGCCAAGGCCTGGAGTGGATGGGCGGC ATCATCCCCATCGTGGGCACCGCCAACTAC GCCCAGAAGTTCCAGGGCAGAGTGACCATC ACAGCCGACGAGTCTACCTCCACCGCTTAT ATGGAACTGTCTTCTCTGCGGTCCGAGGAC ACCGCTGTGTACTACTGTGCTAGAGATTCT AGATACTGCTCCGGCGGCAGCTGCTACTCC GTGTGGTTTGACCCTTGGGGCCAGGGAACC CTGGTGACAGTGTCCTCT Antibody 409_ll_l_vVH (nt-CO)704 GAAGTGCAACTAGTGCAAAGTGGTGCAGA AGTCAAGAAGCCTGGATCTTCTGTGAAAGT GTCCTGCAAGGCCTCTGGCGGACCATTTAG CTCCTCTGCCATCTCCTGGGTGCGGCAGGCT CCTGGCCAAGGCCTGGAGTGGATGGGCGGC ATCATCCCCATGGTGGGCACCGCCAACTAC GCCCAGAAGTTCCAGGGCAGAGTGACCATC ACCGCTGACGAGTCCACCTCCACAGCCTAC ATGGAACTGTCCAGCCTGAGATCCGAGGAC ACCGCTGTGTACTACTGTGCTAGAGATTCTC GGTATTGCTCCGGCGGCTCTTGCTACTCCGT GTTCTTCGACCCTTGGGGCCAGGGCACCCT GGTGACCGTGTCTAGC Antibody 409_ll_l_vVH (nt-CO)705 GAAGTGCAACTAGTGCAAAGTGGTGCAGA AGTCAAGAAGCCTGGCTCCTCTGTGAAAGT GTCCTGCAAGGCCTCTGGCGGCCCATTTAG CAGCTCTGCCATCTCCTGGGTGCGGCAGGC TCCTGGACAAGGCCTGGAGTGGATGGGCGG CATCATCCCCATCGTGGGCACAGCTAACTA CGCCCAGAAGTTCCAGGGCAGAGTGACCAT CACCGCCGACGAGTCCACATCTACCGCCTA CATGGAACTGTCCTCCCTGAGATCCGAGGA CACCGCTGTGTACTACTGTGCTAGAGATTCT CGGTATTGCTCCGGCGGATCTTGCTACTCCG 256 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence TGTTCTTCGACCCTTGGGGCCAGGGCACCC TGGTGACCGTGTCTAGC Antibody 409_ll_2_vl VH (nt - CO)706 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACCA CCTCTGGAGTCGGCGTGGGCTGGATCCGGC AGCCTCCAGGCAAGGCCCTGGAGTACCTGG CTCTGATCTACTGGGACGACGATAAACGGT ATAGCACTAGCCTGAAGTCCAGACTGACAA TCACCAAGGATACATCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ACACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCTCT Antibody 409_ll_2_vVH (nt - CO)707 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACTTTTTCTGGCTTCTCCGTGACCA CCTCCGGCGTGGGAGTGGGCTGGATCAGAC AGCCTCCAGGCAAGGCCCTGGAGTACCTGG CTCTGATCTACTTCGATGATGACAAGCGGT ATAGCACCTCTCTGAAATCTCGGCTGACAA TCACCAAGGACACATCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ACACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)708 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACTTTTAGCGGCTTCTCCGTGACCA CCTCTGGAGTCGGCGTGGGCTGGATCCGGC AGCCTCCTGGCAAAGCTCTGGAGTACCTGG CCCTGATCTACTACGACGATGACAAGCGGT ATAGCACATCTCTGAAGTCCAGACTGACCA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCAGTGG ATACAGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCTCT Antibody 409_ll_2_vVH (nt - CO)709 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAACCAACACAGACCCTGACACTG ACCTGTACTTTTAGCGGCTTCTCCGTGACCA CCTCTGGCGTGGGAGTGGGCTGGATCAGAC 257 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AGCCTCCTGGCAAGGCCCTGGAATATCTCG CTCTGATCTACTGGGAGGACGACAAGCGGT ACTCCACCAGCCTGAAGTCTCGGCTGACCA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGATCCTGTGG ATACAGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCTCT Antibody 409_ll_2_vVH (nt - CO)710 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAGCCTACACAGACCCTGACACTG ACCTGTACTTTTTCTGGCTTCTCCGTGACCA CCAGCGGCGTGGGCGTGGGCTGGATCCGGC AGCCTCCAGGAAAAGCTCTCGAGTACCTGG CCCTGATCTACTGGAACGACGACAAGCGGT ACTCCACCTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGATCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCAGCATCTTCGACTATTGGGGCC AGGGCATCCTGGTCACCGTGTCCTCT Antibody 409_ll_2_vVH (nt - CO)711 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACTTTTAGCGGCTTCTCCGTGACCA CATCTGGCGTGGGAGTGGGCTGGATCAGAC AGCCTCCAGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTGGGACGAGGACAAGCGGT ATTCCACCTCTCTGAAATCTCGGCTGACAAT CACCAAGGACACCTCCAAGAACCAAGTGGT GCTGACCATGACCAACATGGATCCTGTGGA TACCGCTACCTACTACTGCGCCAGACACAC CATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)712 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAACCAACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACCA CCAGCGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTCGAGTACCTGG CTCTGATCTACTGGGACAACGACAAGCGGT ATAGCACTTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGATCCTGTGG ATACAGCTACCTACTACTGCGCCAGACACA 258 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCTCT Antibody 409_ll_2_vVH (nt - CO)713 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACACAGACCCTGACACTG ACCTGTACTTTTTCTGGCTTCTCCGTGACCA CCAGCGGCGTGGGAGTGGGCTGGATCAGAC AGCCTCCAGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTTCGAGGACGACAAGCGGT ATTCCACCTCTCTGAAATCTCGGCTGACAAT CACCAAGGACACCTCCAAGAACCAAGTGGT GCTGACCATGACCAACATGGATCCTGTGGA TACCGCTACCTACTACTGCGCCAGACACAC CATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)714 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAACCAACCCAGACCCTGACACTG ACCTGTACCTTCTCCGGCTTTTCTGTGACCA CCTCTGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTCGAGTACCTGG CTCTGATCTACTTCAACGACGACAAGCGGT ATAGCACTAGCCTGAAGTCCAGACTGACAA TCACCAAGGATACATCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGATCCTGTGG ACACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCTCT Antibody 409_ll_2_vlVH (nt - CO)715 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAGCCTACCCAGACCCTGACACTC ACATGTACCTTTTCTGGCTTCTCCGTGACCA CTAGCGGCGTGGGAGTGGGCTGGATCCGGC AACCACCTGGCAAAGCTCTGGAATACCTGG CCCTGATCTACTACGAGGACGACAAGCGGT ATTCTACCTCTCTGAAGTCCAGACTGACCAT CACCAAGGATACATCCAAGAACCAGGTGGT GCTGACCATGACCAACATGGATCCTGTGGA CACCGCTACCTACTACTGCGCCAGACACAC CATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vll VH (nt - CO)716 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACCA CATCTGGAGTGGGCGTGGGCTGGATCAGAC 259 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AGCCTCCAGGCAAGGCCCTCGAGTACCTGG CTCTGATCTACTACAACGACGACAAGCGGT ATTCCACCTCTCTGAAAAGCCGGCTGACTA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGATCCTGTGG ATACAGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vlVH (nt - CO)717 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAACCAACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACAA CTAGCGGAGTCGGCGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTTCGATGAGGACAAGCGGT ATAGCACCTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCTCT Antibody 409_ll_2_vlVH (nt - CO)718 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACCA CCAGCGGCGTCGGCGTGGGCTGGATCCGGC AGCCTCCAGGAAAAGCTCTGGAGTACCTGG CCCTGATCTACTTCGACAACGACAAGCGGT ATTCTACTTCTCTGAAGTCCAGACTGACCAT CACCAAGGACACCTCCAAGAACCAAGTGGT GCTGACCATGACCAACATGGATCCTGTGGA TACCGCTACCTACTACTGCGCCAGACACAC AATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCAGC Antibody 409_ll_2_vlVH (nt - CO)719 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACATGTACTTTTTCTGGCTTCTCCGTGACCA CCAGCGGAGTCGGCGTGGGCTGGATCAGAC AGCCTCCAGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTACGACGAGGACAAGCGGT ATTCCACCTCTCTGAAATCTCGGCTGACAAT CACCAAGGACACCTCCAAGAACCAAGTGGT GCTGACCATGACCAACATGGATCCTGTGGA TACCGCTACCTACTACTGCGCCAGACACAC 260 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCAGC Antibody 409_ll_2_vlVH (nt - CO)720 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAGCCCACACAGACCCTGACACTG ACCTGCACCTTTTCTGGCTTCTCCGTGACCA CCTCCGGCGTGGGAGTGGGCTGGATCAGAC AGCCTCCAGGCAAAGCTCTCGAGTACCTGG CCCTGATCTACTACGACAACGACAAGCGGT ACTCCACCTCTCTGAAGTCTCGGCTGACTAT CACCAAGGATACAAGCAAGAACCAAGTGG TGCTGACCATGACCAACATGGATCCTGTGG ACACCGCTACCTACTATTGTGCCAGACACA CCATCCCTTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vlVH (nt - CO)721 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACACAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACCA CAAGCGGAGTCGGCGTGGGCTGGATCCGGC AGCCTCCAGGCAAAGCTCTGGAATACCTGG CCCTGATCTACTGGGAGGAGGACAAGCGGT ATTCCACCTCTCTGAAGTCCAGACTGACTAT CACCAAGGACACCTCCAAGAACCAAGTGGT GCTGACCATGACCAACATGGACCCTGTGGA TACCGCTACCTACTACTGCGCCAGACACAC CATCCCCTCTATCTTCGACTACTGGGGCCAG GGCATCCTGGTGACCGTGTCCAGC Antibody 409_ll_2_vlVH (nt - CO)722 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAACCAACCCAGACCCTGACACTG ACCTGTACCTTTAGCGGCTTCTCCGTGACCA CCTCTGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTGGGAGAACGACAAGCGGT ATAGCACATCTCTGAAGTCCAGACTGACAA TCACCAAGGACACTTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCTCT Antibody 409_ll_2_vlVH (nt - CO)723 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTCAAACCAACTCAGACCCTGACACTG ACCTGTACCTTTTCCGGCTTCTCCGTGACCA CATCTGGCGTGGGAGTGGGCTGGATCCGGC 261 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AGCCTCCTGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTGGAACGAGGACAAGCGGT ATAGCACCTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCTATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCAGC Antibody 409_ll_2_vlVH (nt - CO)724 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACTTTTTCTGGCTTCTCCGTGACCA CCTCTGGAGTGGGCGTGGGCTGGATCCGGC AGCCTCCAGGCAAGGCCCTCGAGTACCTGG CTCTGATCTACTGGAACAACGACAAGCGGT ATAGCACATCTCTGAAGTCCAGACTGACAA TCACCAAAGATACCTCCAAGAATCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ACACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)725 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAACCAACCCAGACCCTGACACTG ACCTGTACTTTTTCTGGCTTCTCCGTGACCA CAAGCGGAGTGGGCGTGGGCTGGATCAGA CAGCCTCCTGGCAAGGCCCTGGAATACCTG GCTCTGATCTACTTCGAGGAGGACAAGCGG TATTCCACCTCTCTGAAGTCTCGGCTCACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)726 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACCTTTAGCGGCTTCTCCGTGACTA CATCTGGCGTGGGAGTCGGCTGGATCCGGC AGCCTCCAGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTTCGAGAACGACAAGCGGT ATAGCACCTCTCTGAAGTCCAGACTGACAA TCACCAAAGATACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ACACCGCTACCTACTACTGCGCCAGACACA 262 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCTCT Antibody 409_ll_2_vVH (nt - CO)727 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAACCAACCCAGACCCTGACACTG ACCTGTACCTTCTCCGGCTTTTCTGTGACCA CATCTGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTTCAACGAGGACAAGCGGT ATAGCACCTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTG GTGCTGACCATGACCAACATGGACCCTGTG GATACTGCTACCTACTACTGCGCCAGACAC ACCATCCCCTCCATCTTCGACTACTGGGGCC AGGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)728 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAACCAACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACTA CCTCTGGAGTCGGCGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTGGAGTACCTGG CTCTGATCTACTTCAACAACGACAAGCGGT ATAGCACATCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAATCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTGACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)729 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTGGTGAAACCAACCCAGACCCTGACACTC ACATGTACTTTTTCTGGCTTCTCCGTGACCA CAAGCGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCTGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTACGAGGAGGACAAGCGGT ATTCTACCTCTCTGAAGTCCAGACTGACCAT CACCAAGGACACCTCCAAGAACCAAGTGGT GCTGACCATGACCAACATGGACCCTGTGGA TACCGCTACCTACTACTGCGCCAGACACAC CATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)730 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTCAAACCAACCCAGACCCTGACACTG ACCTGTACCTTCTCCGGCTTTTCTGTGACTA CATCTGGCGTGGGAGTGGGCTGGATCCGGC 263 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AGCCTCCTGGCAAGGCCCTGGAATACCTGG CTCTGATCTACTACGAGAACGACAAGCGGT ATTCCACCTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCAGCATCTTCGACTACTGGGGCC AGGGCATCCTGGTGACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)731 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACTA CAAGCGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCAGGCAAAGCTCTGGAATACCTGG CCCTGATCTACTACAACGAGGACAAGCGGT ATTCTACCTCTCTGAAGTCCAGACTGACAA TCACCAAGGACACCTCCAAGAACCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ATACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC Antibody 409_ll_2_vVH (nt - CO)732 CAAATCACTCTAAAGGAAAGTGGTCCAACT CTCGTGAAGCCTACCCAGACCCTGACACTG ACCTGTACCTTTTCTGGCTTCTCCGTGACCA CCTCTGGCGTGGGAGTGGGCTGGATCCGGC AGCCTCCAGGCAAGGCCCTCGAGTACCTGG CTCTGATCTACTACAACAACGACAAGCGGT ATTCTACTAGCCTGAAGTCCAGACTGACAA TCACCAAGGATACATCCAAAAATCAAGTGG TGCTGACCATGACCAACATGGACCCTGTGG ACACCGCTACCTACTACTGCGCCAGACACA CCATCCCCTCCATCTTCGACTACTGGGGCCA GGGCATCCTGGTCACCGTGTCCAGC AntibodyS2D106-vl.lVH(nt)733 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GT GAAGAAGC C T GGGT C C T C ( jGT (i A A G( j IT TCCTGCAAGGCTTCTGGAGGCCCCTTCAG C AGC T C TGC T ATC AGC T GGGT GC GAC AGG CCCCTGGACAAGGGCTTGAGTGGATGGGAG- GGATCATCCCTATGGTAGGTACAGCAAAC TAl'GCACAGAAGTI'CCAGGGCAGAGTCACG ATTACCGCGGACGAATCCACGAGCACAGCC ' f AC A' f GGAGC' f GAGC AGC C T GAG AT C T GA G GACACGGCCGTGTATTACTGTGCGAGAGA TTCGCGCTATTGTAGTGGTGGTAGCTGC 264 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence TACTCCGTCTGGTTCGACCCCTGGGGCCA GGGAACCCTGGTCACCGTCTCCTCA Antibody 409_ll_l_vVH(nt)734 GAGGIGC AGC T GGTGC A GT C T GGGGC T GAG GTGAAGAAGCCTGGGTCCTCGGTGAAGGTT TCCTGCAAGGCTTCTGGAGGCCCCTTCAG CAGCTCTGCTATCAGCTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAG GGATCATCCCTATAGTAGGTACAGCAAAC TATGCACAGAAGTTCCAGGGCAGAGTCACG ATTACCGCGGACGAATCCACGAGCACAGCC T AC AT GGAGC T GAGC -A GC C T GAG AT C T GAG GACACGGCCGTGTATTACTGTGCGAGAGA TTCGCGCTATTGTAGTGGTGGTAGCTGC TACTCCGTCTGGTTCGACCCCTGGGGCCA GGGAACCCTGGTCACCGTCTCCTCA Antibody 409_ll_l_vVH(nt)735 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GTGAAGAAGCCTGGGTCCTCGGTGAAGGTT TCCTGCAAGGCTTCTGGAGGCCCCTTCAG CAGCTCTGCTATCAGCTGGGTGCGACAGG CCCCTGGACAAGGGCTTGAGTGGATGGGAG GGA EGA ICCC IATGG IAGG1ACAGCAAAC TATGCACAGAAGTTCCAGGGCAGAGTCACG AT T A C C GC GGAC GA A T C C A C GAGC AC A GC C TACATGGAGCTGAGCAGCCTGAGATCTGAG GACACGGCCGTGTATTACTGTGCGAGAGA 11CGCGCI AT TG T AG I G G I G G I AGC I GC TACTCCGTCTTCTTCGACCCCTGGGGCCA GGGAACCCTGGTCACCGTCTCCTCA Antibody 409_ll_l_vVH(nt)736 GAGGTGCAGCTGGTGCAGTCTGGGGCTGAG GT GAAGAAGC C T GGGT C C T C ( jGT (i A A GG I T TCCTGCAAGGCTTCTGGAGGCCCCTTCAG CAGCTCTGCTATCAGCTGGGTGCGACAGG CCCCTGGACAA(jG(jC'TrGAGTGGA KjGGAG GGATCATCCCTATAGTAGGTACAGCAAAC TATGCACAGAAGTTCCAGGGCAGAGTCACG ATTACCGCGGACGAATCCACGAGCACAGCC TACATGGAGCTGAGCAGCCTGAGATCTGAG GACACGGCCGTGTATTACTGTGCGAGAGA TTCGCGCTATTGTAGTGGTGGTAGCTGC TACTCCGTCTTCTTCGACCCCTGGGGCCA GGGAACCCTGGTCACCGTCTCCTCAAntibody S2E12-vl.l VL(nt)737GATATTGTGTTGACGCAGACTCCAGGCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTGTTAG CAGCAGTTACTTAGCCTGGTACCAGCAGA 265 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence AACCTGGCCAGGCTCCCAGGCTCCTCATCT ATGGTGCATCCAGCAGGGCCACTGGCATC CCAGACAGGTTCAGTGGCAGTGGGTCTGGG ACAGACTTCACTCTCACCATCAGCAGACTG GAGCCTGAAGATTTTGCAGTGTATTACTGT CAGCAGTATGTTGGCTTAACAGGGTGGA CGTTCGGCCAAGGGACCAAGGTGGAAATC AAAAntibody 409_ll_4_vVL (aa)738DIVLTQTPGTLSLSPGERATLSCRASQSVSSSY LAWYQQKPGQAPRALIYGASSRATGIPDRFS GSGSGTDFTLTISRLEPEDFAVYYCQQYVGL TGWTFGQGTKVEIK Antibody 409_ll_4_vVL(nt)739 GATATTGTGTTGACGCAGACTCCAGGCACC CTGTCTTTGTCTCCAGGGGAAAGAGCCACC CTCTCCTGCAGGGCCAGTCAGAGTGTTAG CAGCAGTTACTTAGCCTGGTACCAGCAGA AACCTGGCCAGGCTCCCAGGGCCCTCATCT ATGGTGCATCCAGCAGGGCCACTGGCATC CCAGACAGGTTCAGTGGCAGTGGGTCTGGG ACAGACTTCACTCTCACCATCAGCAGACTG GAGCCTGAAGATTTTGCAGTGTATTACTGT CAGCAGTATGTTGGCTTAACAGGGTGGA CGTTCGGCCAAGGGACCAAGGTGGAAATC AAA Antibody 409_3_l_vVH(aa)740 QVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGWINPNSGGTN FAQKFQGRVTMTRDTSISTAYMELSRLRSDD TAVYYCASSGYLGYYYYGMDVWGQGTTVT vss Antibody 409_3_l_vVH(aa)741 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGWINPNSGGTN FAQKFQGRVTMTRDTSISTAYMELSSLRSDDT AVYYCASSGYLGYYYYGMDVWGQGTTVTV SS Antibody 409_3_l_vVH(aa)742 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGWINPNSGGTN FAQKFQGRVTMTRATSISTAYMELSRLRSDD TAVYYCASSGYLGYYYYGMDVWGQGTTVT VSS Antibody 409_3_l_vVH(aa)743 EVQLVQSGAEVKKPGASVKVSCKASGYTFT GYYIHWVRQAPGQGLEWMGWINPNSGGTN FAQKFQGRVTMTRDTSFSTAYMELSRLRSDD TAVYYCASSGYLGYYYYGMDVWGQGTTVT VSS 266 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence Antibody 409_ll_4_vVL (aa)744DIVLTQTPGTLSLSPGERATLSCRASQSVS SSYLAWYQQKPGQAPRLLIYGASSRATGIP DRFSGSGSGTDFTLTISRLEPEDFAVYYCQ QYVGLTGFTFGQGTKVEIKAntibody 409_ll_4_v3CDRL3 (aa)745 QQYVGLTGFT Antibody 409_ll_4_vVL (aa)746DIVLTQTPGTLSLSPGERATLSCRASQSVS SSYLAWYQQKPGQAPRLLIYGASSRATGIP DRFSGSGSGTDFTLTISRLEPEDFAVYYCQ QYVGLTGYTFGQGTKVEIKAntibody 409_ll_4_v4CDRL3 (aa)747 QQYVGLTGYT Antibody 409_ll_4_vVH(aa)748 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFTSSAVQWVRQARGQRLEWVGF IVVGSGNTNYAQKFHERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY CSGGSCSDGFDIWGQGTMVTVSS Antibody 409_ll_4_vVH(aa)749 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFTSSAVQWVRQARGQRLEWVGY IVVGSGNTNYAQKFHERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY CSGGSCSDGFDIWGQGTMVTVSS Antibody 409_ll_4_vVH(aa)750 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFTSSAVQWVRQARGQRLEWVGW IVVGSGNTNYAQKFH ERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY CSGGSCSDAFDIWGQGTMVTVSS Antibody 409_ll_4_vCDRH3 and N-terminal Al a-Ser(aa) 751 ASPYCSGGSCSDAFDI Antibody 409_ll_4_vVH(aa)752 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFTSSAVQWVRQARGQRLEWVGW IVVGSGNTNYAQKFHERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY SSGGSSSDGFDIWGQGTMVTVSS 267 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence Antibody 409_ll_4_vCDRH3 and N-terminal Al a-Ser(aa) 753 ASPYSSGGSSSDGFDI Antibody 409_ll_4_vVH(aa)754 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFT SSAVQWVRQARGQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY PSGGSPSDGFDIWGQGTMVTVSS Antibody 409_ll_4_vCDRH3 and N-terminal Al a-Ser(aa) 755 ASPYPSGGSPSDGFDI Antibody 409_ll_4_vlVH(aa)756 QVQLVQSGPEVKKPGTSVRVSCKASGFTFTSSAVQWVRQARGQRLEWVGWIVVGSGNTNYAQKFHERVTITRDMSTSTAMELSSLRSEDTAVYYCASPY ASGGS ASDGFDIWGQGTMVT VS S Antibody 409_ll_4_vlCDRH3 and N-terminal Al a-Ser(aa) 757 ASPYASGGSASDGFDI Antibody 409_ll_4_vll VH(aa)758 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFTSSAVQWVRQARGQRLEWVGF IVVGSGNTNYAQKFH ERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY CSGGSCSEGFDIWGQGTMVTVSS Antibody 409_ll_4_vlVH(aa)759 QVQLVQSGPEVKKPGTSVRVSCKAS GFTFTSSAVQWVRQARGQRLEWVGW IVVGSGNTNYAQKFHERVTITRDMS TSTAYMELSSLRSEDTAVYYCASPY CSGGSCSEGFDIWGQGTMVTVSSAntibody 409_ll_4_vlCDRH3 with N-terminal Al a-Ser(aa) 760 ASPYCSGGSCSEGFDI Antibody761QVQLVQSGPEVKKPGTSVRVSCKASGFTFTS SAVQWVRQARGQRLEWVGAIVVGSGNTNY 268 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence 409_ll_4_vlVH(aa)AQKFHERVTITRDMSTSTAYMELSSLRSEDTAVYYCASPYCSGGSCSDGFDIWGQGTMVTVS S Antibody 409_ll_l_vVH(aa)762 EVQLVQSGAEVKKPGSSVKVSCKASGGPFSS SAISWVRQAPGQGLEWMGGIIPMVGTANYA QKFQGRVTITADESTSTAYMELSSLRSEDTAV YYCARDSRYASGGSAYSVWFDPWGQGTLV TVSSAntibody 409_ll_l_vCDRH3 (aa)763 ARDSRYASGGSAYSVWFDP Antibody 409_ll_l_vVH(aa)764 EVQLVQSGAEVKKPGSSVKVSCKASGGPFSS SAISWVRQAPGQGLEWMGGIIPMVGTANYA QKFQGRVTITADESTSTAYMELSSLRSEDTAV YYCARDSRYSSGGSSYSVWFDPWGQGTLVT vss Antibody 409_ll_l_vCDRH3 (aa)765 ARDSRYSSGGSSYSVWFDP SARS-CoV-2S2E12-vl mAb CDRH(IMGT) (aa)766 PYCSGGSCSDGFDI SARS-CoV-S2E12-vheavy chain (MLNS)767 QVQLVQSGPEVKKPGTSVRVSCKASGFTFTS SAVQWVRQARGQRLEWVGWIVVGSGNTNY AQKFHERVTITRDMSTSTAYMELSSLRSEDTA VYYCASPYCSGGSCSDGFDIWGQGTMVTVS SASTKGPSVFPLAPSSKSTSGGTAALGCLVKD YFPEPVTVSWNSGALTSGVHTFPAVLQSSGL YSLSSVVTVPSSSLGTQTYICNVNHKPSNTKV DKKVEPKSCDKTHTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVVVDVSHEDPEVKF NWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVLHE ALHSHYTQKSLSLSPGK SARS-CoV-409_ll_4_vVL light chain768DIVLTQTPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRALIYGASSRATGIPDRFS GSGSGTDFTLTISRLEPEDFAVYYCQQYVGL TGWTFGQGTKVEIKRTVAAPSVFIFPPSDEQL 269 WO 2021/226560 PCT/US2021/031442 Sequence Description SEQ ID NO. Sequence I SARS-CoV-409_ll_4_vCDRH(IMGT) (aa)770 PYSSGGSSSDGFDI SARS-CoV-409_ll_4_vCDRH(IMGT) (aa)771 PYPSGGSPSDGFDI SARS-CoV-409_ll_4_vlCDRH(IMGT) (aa)772 PYASGGSASDGFDI SARS-CoV-409_ll_4_vll and 409_ll_4_vlCDRH(IMGT) (aa) 773 PYCSGGSCSEGFDI 270 WO 2021/226560 PCT/US2021/031442 EXAMPLES EXAMPLE 1 Human Antibodies Binding SARS-CoV-2 Spike Protein Monoclonal antibodies were isolated from human patients who recovered from SARS-CoV-2 infection. Briefly, EBV-immortalized memory B cells were sorted based on binding to the full ectodomain of SARS-CoV-2 Spike protein in the trimeric prefusion conformation. A biotin moiety was attached to the C-terminus of the Spike protein and the biotinylated Spike was coupled to fluorescent streptavidin (AF6fluorophore) and used to stain the B cells, which were then sorted for binding to trimeric prefusion Spike protein based on fluorescence. Monoclonal antibodies identified by this process were recombinantly expressed in ExpiCHO cells transiently co-transfected with plasmids expressing the heavy and light chains.

EXAMPLE 2 Binding of Antibodies to SARS-CoV-1 RBD and SARS-CoV-2 RBD Binding of monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection to the RBD of SARS-CoV-1 and SARS-CoV-2 Spike protein was assessed using enzyme-linked immunosorbent assays (ELISA).Briefly, 96-well plates were coated with SARS-CoV-2 RBD (produced in- house; residues 331-550 of spike from BetaCoV/Wuhan-Hu-1/2019, accession number MN908947), or SARS-C0V (also described herein as SARS-CoV-1) RBD (Sino Biological). Wells were washed and blocked with PBS+1%BSA for 1 hour at room temperature and were then incubated with serially diluted recombinant monoclonal antibodies for 1 hour at room temperature. Bound antibodies were detected by incubating alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotechnology: 2040-04) for 1 hour at room temperature and were developed by mg/ml p-nitrophenylphosphate substrate in 0.1 M glycine buffer (pH 10.4) for 271 WO 2021/226560 PCT/US2021/031442 minutes at room temperature. The optical density (OD) values were measured at a wavelength of 405 nm in an ELISA reader (Powerwave 340/96 spectrophotometer, BioTek).ELISA assay results are shown in Figures 1A-1D. In each figure, binding toRBD of SARS-CoV-2 is shown in the top panel and binding to RBD of SARS-CoV-1 is shown in the bottom panel. Calculated EC50 values (in ng/ml) are shown in the boxes on the right side of the figures and in Table 3.

Table 3. Binding (EC50 ng/ml) of Antibodies to SARS RBDs (ELISA) Antibody EC50: SARS-CoV-2 RBD EC50: SARS-CoV-1 RBD S2X11 18.70 -S2X14 26.57 -S2X16 17.02 -S2X19 19.44 -S2X21 20.38 -S2X25 25.69 -S2X28 - -S2X30 37.94 -S2X33 34.01 -S2X35 48.26 84.64S2X41 20.01 -S2X42 23.45 26.08S2X47 92.02 -S2X52 14.18 -S2X55 13.75 -S2X56 12.73 -S2X58 22.91 -S2X59 12.56 -S2X67 17.91 -S2X71 11.53 -S2X73 12.03 - 272 WO 2021/226560 PCT/US2021/031442 Antibody EC50: SARS-CoV-2 RBD EC50: SARS-CoV-1 RBD S2X76 11.73 -S2X78 14.34 - Further assays using the same procedure were carried out with additional antibodies. Results are shown in Figures 8A and 8B. In each of these figures, binding to SARS-CoV-2 RBD is shown in the left panel and binding to SARS-CoV-1 RBD is shown in the right panel. Calculated EC50 values (in ng/ml) are shown in the boxes on the right side of each panel.The same procedures were carried out with other antibodies. Results are shown in Figures 10A-10E. In each of these figures, binding to SARS-CoV-2 RBD is shown in the top panel and binding to SARSRBD of SARS-CoV-1 is shown in the bottom panel. Calculated EC50 values (in ng/ml), where available, are shown in the boxes on the right side of each panel. In this assay, antibody S2E12 (VH amino acid sequence of SEQ ID NO. :399; VL amino acid sequence of SEQ ID NO. :403) bound SARS-CoV-RBD with an EC50 of 43.40 ng/ml.Binding of additional antibodies to SARS-CoV-2 RBD was determined using similar methods. Results are shown in Figures 22A and 22B. Calculated EC50 values are shown in the boxes to the right of each graph.

EXAMPLE 3 Binding of Antibodies to SARS-CoV-1 and SARS-CoV-2 Spike Protein AND TO SARS-CoV-1 AND SARS-CoV-2 RBD Binding of certain human monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection to the Spike protein of SARS-CoV-2, and to the RBD of SARS-CoV-1 and SARS-CoV-2 Spike protein, was assessed by enzyme-linked immunosorbent assays (ELISA).Briefly, 96-well plates were coated with SARS-C0V Spike SI Subunit Protein (Sino Biological), SARS-CoV-2 RBD (produced in house; residues 331-550 of spike 273 WO 2021/226560 PCT/US2021/031442 from BetaCoV/Wuhan-Hu-1/2019, accession number MN908947), or SARS-C0V RBD (Sino Biological). Wells were washed and blocked with PBS+1%BSA for 1 hour at room temperature and were then incubated with serially diluted recombinant monoclonal antibodies for 1 hour at room temperature. Bound antibodies were detected by incubating alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotechnology: 2040-04) for 1 hour at room temperature and were developed by mg/ml p-nitrophenylphosphate substrate in 0.1 M glycine buffer (pH 10.4) for minutes at room temperature. The optical density (OD) values were measured at a wavelength of 405 nm in an ELISA reader (Powerwave 340/96 spectrophotometer, BioTek).ELISA assay results are shown in Figures 4A-4N. Calculated EC50 values (in ng/ml) are shown in the boxes on the right side of each figure.Binding of additional monoclonal antibodies to Spike protein of SARS-C0V-and SARS-C0V-2 and to Spike protein RBD of SARS-CoV-1 and SARS-C0V-2 was assessed using similar methods. Results are shown in Figures 18A-18E. Calculated EC50 values (ng/ml) are shown in the boxes on the right side of each figure.Binding of additional monoclonal antibodies to SARS-CoV-1 Spike protein, SARS-CoV-1 Spike RBD, and SARS-C0V-2 Spike RBD was determined by similar methods. Results are shown in Figures 20A, 20B, 21 A, and 21B. Boxes to the right of the graphs show calculated EC50 values (ng/ml).

EXAMPLE 4 Competitive Binding of Antibodies With Human ACE2 For Binding RBD Competitive binding of recombinant monoclonal antibodies and human ACEto RBD was measured by competition ELISA. Recombinant antibodies were produced using monoclonal antibodies isolated from patients who recovered from SARS-C0V-infection. 274 WO 2021/226560 PCT/US2021/031442 Briefly, ELISA plates were coated with recombinant human ACE2 (produced in-house) Coating was carried out with ACE2 at 2ug/ml in PBS. Plates were incubated overnight at 4°C and blocking was performed with blocker Casein (1% Casein from Thermofisher) for 1 hour at room temperature. Serial dilutions of monoclonal antibodies were incubated with SARS-CoV-2 RBD at 20ng/ml (RBD fused with mouse Fc, from Sino Biological) for 30 minutes at 37°C and then transferred onto the ACE2- coated plates for an additional incubation at room temperature. Plates were washed and binding of RBD to ACE2 was detected using a polyclonal goat anti-mouse Fc-AP antibody (Southern Biotech). After an additional wash, AP substrate pNPP (Sigma) was added and plates were incubated at 20 minutes at room temperature before measuring adsorbance at 405nm with a spectrophotometer (Powerwave340 Biotek). Results are shown in Figure 2.Further assays were carried out using similar methods for additional monoclonal antibodies. Results are shown in Figures 13A, 13B, 23A, and 23B. In these figures, calculated IC50 values are shown to the right of each graph.

EXAMPLE 5 Neutralization of SARS-CoV-2 Pseudotyped MLV by Recombinant Human Monoclonal Antibodies Monoclonal antibodies isolated from patients who recovered from SARS-CoV-infection were expressed recombinantly and tested in neutralization assays against SARS-CoV-2 pseudotyped virus.Briefly, Murine leukemia virus (MLV) pseudotyped with SARS-CoV-2 Spike protein (SARS-C0V-2pp) was used. VeroE6 cells were used as target cells and were seeded one day before addition of virus and antibodies. SARS-C0V-2pp was activated with trypsin TPCK at lOug/ml. Activated SARS-C0V-2pp was added to a dilution series of antibodies and incubated for 48 hours. Starting concentration for antibodies was 5ug/ml per antibody, 3-fold dilution. Luminescence was measured after aspirating cell culture supernatant and adding Bio-Gio substrate (Promega). 275 WO 2021/226560 PCT/US2021/031442 Results are shown in Figures 3A-3F. Calculated IC50 values, in ng/ml, are shown on the right of each of these figures. Table 4 shows the calculated IC50, IC80, and IC90 values, in ng/ml.

Table 4. Neutralization against MLV Pseudotyped with SARS-C0V-2 S Protein ng/ml Antibody IC50 IC80 IC90 S2X47 3 12 25S2X16 6 22 46S2X56 8 38 94S2X55 11 31 57S2X30 15 31 46S2X58 19 40 62S2X76 11 37 74S2X71 12 48 109S2X11 28 64 103S2X28* 13 573 5185S2X19 22 80 169S2X14 39 127 255S2X52 37 134 284S2X41 41 140 285S2X78 29 122 285S2X21 42 138 279S2X35 92 320 661S2X33 35 412 1734S2X25 79 532 1624S2X73 41 229 628S2X59 52 234 563S2X67 45 225 573S2X42 — — — Further monoclonal antibodies isolated from patients who recovered fromSARS-C0V-2 infection were expressed recombinantly and tested in neutralization assays against SARS-C0V-2 pseudotyped virus using the same procedure. Results are shown in Figures 5A-5D and Figures 7A-7D. The antibody labeled "S2H58" in Figure 7D comprises the VH amino acid sequence set forth in SEQ ID NO.: 228 and the VL amino acid sequence set forth in SEQ ID NO.: 238. Calculated EC50 values for theantibodies in Figures 5A-5D are shown in the box to the right of each figure. 276 WO 2021/226560 PCT/US2021/031442 Calculated EC50 and EC90 values for the antibodies in Figures 7A-7D are shown in Table 5.

Table 5. Neutralization against MLV Pseudotyped with SARS-CoV-2 S Protein Antibody EC50 (ng/ml) EC90 (ng/ml) SN12 37.22 79.099S2N19 98.71 516.291S2N22 36.19 58.835S2N24 65.53 167.841S2N25 39.22 88.528S2N27 55.97 129.421S2N28 32.72 174.533S2N41 0.1125 -S2X11 50.77 245.392S2X71 19.09 174.137S2X76 33.19 67.599S2H58 9.653 54.544S2H67 60.12 221.856S309 727.2 1472.870 Additional monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested in neutralization assays against SARS-CoV-2 pseudotyped virus using the same procedure. Results are shown in Figures 9A-9F. Calculated IC50, IC80, and IC90 values are shown below the graph in each figure. Antibody S2E12 shown in Figure 9E comprises the VH amino acid sequence of SEQ ID NO.:399 (CDRH1-H3 of SEQ ID NOs.:400, 401, and 766, respectively) and the VL amino acid sequence of SEQ ID NO.:403 (CDRL1-CDRL3 of SEQ ID NOs. :404-406, respectively).Neutralization of infection by other antibodies was assayed using similar methods. Results are shown in Figures 11 A-l ID. Calculated IC50, IC80, and ICvalues are shown on the right of each figure. 277 WO 2021/226560 PCT/US2021/031442 Other antibodies were tested in neutralization assays against SARS-CoV-pseudotyped virus using similar methods. Results are shown in Figures 17A-17C. Calculated IC50, IC80, and IC90 values are shown below each graph.Other antibodies were tested in neutralization assays against SARS-CoV-pseudotyped virus using similar methods. Results are shown in Figures 19A-19E. Calculated IC50, IC80, and IC90 values are shown to the right the graph in each figure.

EXAMPLE 6 Antibody Neutralization of Live SARS-CoV-2 Monoclonal antibodies isolated from patients who recovered from SARS-CoV-infection were expressed recombinantly and were tested in neutralization assays against live SARS-CoV-2 virus.Briefly, Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and lx Penicillin/Streptomycin (Thermo Fisher Scientific) were seeded in white 96-well plates at 20,000 cells/well and attached overnight. Serial 1:4 dilutions of the monoclonal antibodies were incubated with 200 pfu of SARS-CoV-2 (isolate USA- WA1/2020, passage 3, passaged in Vero E6 cells) for 30 minutes at 37°C in a BSL-facility. Cell supernatant was removed and the virus-antibody mixture was added to the cells. 24 hours post infection, cells were fixed with 4% paraformaldehyde for minutes, followed by two PBS (pH 7.4) washes and permeabilization with 0.25% Triton X-100 in PBS for 30 minutes. After blocking in 5% milk powder/PBS for 30 minutes, cells were incubated with a primary antibody targeting SARS-CoV-2 nucleocapsid protein (Sino Biological, cat. 40143-R001) at a 1:2000 dilution for !hour. After washing and incubation with a secondary Alexa647-labeled antibody mixed with ug/ml Hoechst33342 for 1 hour, plates were imaged on an automated cell-imaging reader (Cytation 5, Biotek) and nucleocapsid-positive cells were counted using the manufacturer ’s supplied software. Data were processed using Prism software (GraphPad Prism 8.0). 278 WO 2021/226560 PCT/US2021/031442 Results are shown in Figures 6A and 6B, and Tables 6 and 7 (EC50 and ECvalues, ng/ml). Calculated IC50 values (ng/ml) are shown in Table 8. 279 Table 6. 280 X (Interpolated)S309-vLS (entered) S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered) 287.351 90.00081.753 50.00037.711 90.0007.417 50.00024.483 90.0006.720 50.00072.423 90.00015.551 50.00018.972 90.0005.243 50.00034.097 90.0007.570 50.000376.690 90.00094.413 50.00053.786 90.0007.373 50.000 W O 2021/226560 PCT/US2021/031442 Table 7.

X (Interpolated)S309-vLS (entered) S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered) 29.826 90.00011.470 50.00019.539 90.0006.693 50.000 281 X (Interpolated)S309-vLS (entered) S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered) 452.707 90.000199.976 50.000110.659 90.00024.449 50.000351.094 90.000132.514 50.000364.145 90.00095.154 50.000228.754 90.000 W O 2021/226560 PCT/US2021/031442 282 X (Interpolated)S309-vLS (entered) S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered)S2X(entered) 58.650 50.000108.265 90.00034.773 50.000156.012 90.00036.085 50.000840.691 90.000243.758 50.000159.046 90.00035.974 50.000101.959 90.00034.220 50.00086.467 90.00023.224 50.000 W O 2021/226560 PCT/US2021/031442 WO 2021/226560 PCT/US2021/031442 Table 8. Antibody IC50Experiment 1IC90Experiment 1IC50Experiment 2IC90Experiment 2S309-v2 LS 81.75 297 201.8 452S2X71 7.417 32 24.59 110S2X30 6.720 24 134.4 351S2X56 15.49 72 94.92 364S2X58 - - 58.09 228S2X76 5.243 19 34.56 108S2X16 7.512 34 35.55 156S2X35 94.41 377 237.4 840S2X28 7.140 54 35.75 159S2X55 11.44 30 35.48 102S2X11 6.679 20 22.53 86 Twenty-two further antibodies, along with comparator antibody S309-v2 (VH as set forth in SEQ ID NO.: 342, VL as set forth in SEQ ID NO.: 346 (CDRH1-H3 andL1-L3 as set forth in SEQ ID NOs. :343-345 and 347-349, respectively)), were tested in neutralization assays against live SARS-CoV-2 virus using similar methods. Results are shown in Figures 16A-16D. S2H58-v2 comprises the VH amino acid sequence set forth in SEQ ID NO: 228 and the VL amino acid sequence (kappa light chain) set forth in SEQ ID NO: 238. S2E12 comprises the VH amino acid sequence set forth in SEQID NO. :399 (CDRH1-H3 as set forth in SEQ ID NOs. :400, 401, and 766, respectively), and the VL amino acid sequence set forth in SEQ ID NO. :403 (CDRL1-L3 as set forth in SEQ ID NOs. :404-406, respectively). Calculated IC50 (ng/ml) values are shown in Tables 9-12. Calculated EC50 and EC90 values (ng/ml) are shown in Tables 13-16.

Table 9. Antibody IC50 S309-v2 123.8S2N22 13.58 283 WO 2021/226560 PCT/US2021/031442 Antibody IC50 S2N12 23.68S2N28 17.59S2N25 13.64S2H58-v2 6.337 Table 10. Antibody IC50 S309-v2 145.7S2E9 24.93S2E6 13.52S2E13 approx. 14.02S2K4 26.36S2E14 8.300S2E7 24.43S2E12 5.409 Table 11. Antibody IC50 S309-v2 146.0S2H37 48.67S2H73 12.72S2H40 18.63S2H70 52.69S2H71 5.580 Table 12. Antibody IC50 S309-v2 173.7S2X30 11.42S2H58-V1 8.362 284 WO 2021/226560 PCT/US2021/031442 Antibody IC50 S2H66 633.0S2H62 24.17S2H30 28.80 Table 13. Antibody EC50 EC90 S309-v2 126 438S2N22 13 46S2N12 25 77S2N28 18 44S2N25 13 49S2H58-v2 6 19 Table 14. Antibody EC50 EC90 S309-v2 145 342S2E6 16 70S2E9 25 66S2E13 14 17S2K4 13 49S2E14 9 41S2E12 5 16 Table 15. Antibody EC50 EC90 S309-v2 (with M428L andN434S Fc mutations)151 461 S2H37 49 169S2H73 13 65S2H40 20 65 285 WO 2021/226560 PCT/US2021/031442 S2H70 52 234S2H71 6 20 Table 16. Antibody EC50 EC90 S309-v2 (with M428L andN434S Fc mutations)174 588 S2X30 12 34S2H58 8 23S2H66 584 5000S2H62 24 82S2H30 27 121 Neutralization assays were carried out using additional monoclonal antibodiesusing similar methods. Results are shown in Figures 25A and 25B. Figure 25A shows results for four antibodies, along with comparator antibodies S309 N55Q LS and S2X193. S309 N55Q LS (also referred-to herein as S309-v2) comprises the VH sequence as set forth in SEQ ID NO:342 and the VL sequence as set forth in SEQ ID NO: 346, and comprises M428L and N434S mutations in the Fc region. Figure 25Bshows results for antibodies S2X129 and S2X132, along with four comparator antibodies. IC50 and interpolated EC50 and EC90 values (ng/ml) are shown in Table 17.

Table 17. Antibody IC50 EC50 EC90 S309 N55Q_MLNS (2 measurements)268.4; 205.4 256; 194 635; 634 S2X127 17.18 17 54S2X129(2 measurements)5.379; 4.1045; 412; 12 S2X132 16.50; 9.265 16; 9 38; 24 286 WO 2021/226560 PCT/US2021/031442 Antibody IC50 EC50 EC90 (2 measurements)S2X190 24.18 23 64S2X193 26.69 26 61S2X195 17.85 17 47S2X219 20.63 20 69S2X246 7.894 8 22 Neutralization by other antibodies (expressed as recombinant IgGl with wild- type or M428L/N434S ("LS")-modif1ed Fc) was assessed using VSV-luc(spike D19) pseudovirus. Plots are shown in Figure 26; IC50 values and interpolated EC50 andEC90 values (ng/ml) are shown in Table 18.

Table 18. Antibody IC50 EC50 EC90 S309 (wt) 25.69 25.8 121S2E12-LS 1.401 1.4 11.9S2Mll-vl-LS 0.9143 1.1 14.4S2D106-LS 3.376 3.6 15.4409_ll_3_vl-LS 4.085 5.3 40.9S2X227-v2-LS 4.446 5.3 27.5409_ll_2_vl-LS 2.327 2.4 9.3 These antibodies were also tested for neutralization of live SARS-CoV-2. Plots are shown in Figure 27; data are from triplicate wells SARS-C0V-2-luc, MOI 0.1, 6hinfection. IC50 values and interpolated EC50 and EC90 values (ng/ml) are shown in Table 19.

Table 19. Antibody IC50 EC50 EC90 S2E12-LS 4.844 5 30S2Mll-vl-LS 3.214 4 11 287 WO 2021/226560 PCT/US2021/031442 Antibody IC50 EC50 EC90 S2D106-LS 4.485 6 22409_ll_3_vl-LS 8.233 10 39S2X227-v2-LS 7.061 10 44409_ll_2_vl-LS 5.073 5 20S309 84.30 79 289 EXAMPLE 7 Production of S2X16, S2X30, S2X35, and S2X47 Variant Antibodies Recombinant IgGl antibodies are produced using the VH and VL sequences of antibodies S2X16, S2X30, S2X35, and S2X47, or engineered variants thereof. Thecombinations are produced as indicated in Table 20. Each of the antibodies is produced by transient transfection and expression of a plasmid vector encoding the recombinant antibody in HD 293F cells (GenScript). Cells are harvested on day 4 and IgG expression is validated by Western blot and protein A titer analysis.

Table 20. Antibody VH (WT/V ariant) VH amino acid SEQ ID NO. VL (WT/V ariant) VL amino acid SEQ ID NO. S2X16-11 WT 22 WT 26S2X16-21 W50F 150 WT 26S2X16-12 WT 22 N33Q 154S2X13-23 W50F 150 G34A 157S2X30-11 WT 42 WT 46S2X30-21 D112E 163 WT 46S2X30-12 WT 42 W94F 168S2X35-11 WT 129 WT 133S2X35-21 W50F 173 WT 133S2X35-31 W109F 175 WT 133 288 WO 2021/226560 PCT/US2021/031442 S2X35-41 W50F-W109F-I110A178 WT 133 S2X47-11 WT 52 WT 56S2X47-21 W111F 186 WT 56S2X47-12 WT 52 W92F-W99F 194S2X47-33 W36F 189 W92F 196S2X47-42 W36F-W111F 191 W92F-W99F 194 EXAMPLE 8 S2H58 and S2N22 Variant Antibodies Recombinant IgGl antibodies are produced using the VH and VL sequences of monoclonal antibodies S2H58 and S2N22, or engineered variants thereof. The combinations are produced as indicated in Table 21. Each of the antibodies is produced by transient transfection and expression of a plasmid vector encoding the recombinant antibody in HD 293F cells (GenScript). Cells are harvested on day 4 and IgG expression is validated by Western blot and protein A titer analysis. Table 21. Antibody VH (WT/Variant) VH amino acid SEQ ID NO. VL (WT/V ariant) VL amino acid SEQ ID NO. S2H58-11 WT 228 WT 238S2H58-21 W50F 350 WT 238S2H58-31 W50F-S55A 351 WT 238S2H58-32 W50F-S55A 351 V34G 355S2H58-43 W50F-S55A-LI 02 A353 M94I 357 S2N22-11 WT 312 WT 316S2N22-21 N56Q 359 WT 316S2N22-31 N56G 361 WT 316 289 WO 2021/226560 PCT/US2021/031442 Antibody VH (WT/Variant) VH amino acid SEQ ID NO. VL (WT/V ariant) VL amino acid SEQ ID NO. S2N22-41 T58A 363 WT 316S2N22-51 Y57P 365 WT 316S2N22-61 T58A-S63A 367 WT 316S2N22-71 T58A-S63A-M34I 368 WT 316 EXAMPLE 9 S2E12 Variant Antibodies Recombinant IgGl antibodies were produced using the VH and VL sequences of monoclonal antibody S2E12 and engineered variants thereof. V-region amino acid sequences of S2E12 and certain engineered S2E12 variants are summarized in Table22.

Table 22. Antibody VH amino acid SEQ ID NO. VL amino acid SEQ ID NO. S2E12 399 403409 11 4 v2 399 738409 11 4 v3 399 744409 11 4 v4 399 746409 11 4 v5 748 403409 11 4 v6 749 403409 11 4 v7 750 403409 11 4 v8 752 403409 11 4 v9 754 403409 11 4 vlO 756 403409 11 4 vll 758 403409 11 4 vl2 759 403409 11 4 vl3 761 403 290 WO 2021/226560 PCT/US2021/031442 EXAMPLE 10 Neutralization of SARS-CoV-2 by Recombinant Antibodies Human monoclonal antibodies isolated from patients who recovered from SARS-CoV-2 infection were expressed recombinantly and tested in neutralization assays against SARS-CoV-2 pseudotyped virus (VSV).Recombinant monoclonal antibodies were serially diluted and incubated with a constant amount of VSV-deltaG-luc pseudotyped with SARS-CoV-2 (strain BetaCoV/Wuhan-Hu-1/2019, accession number MN908947) for 1.5 hours at 37 °C. VeroE6 cells were then added in complete DMEM medium and plates were incubated for 24 hours at 37 °C. To measure the amount of luciferase expressed in infected cells, culture medium was aspirated and luciferase substrate Bio-Gio Luciferase assay system (Promega AG) warmed to room temperature was added. After 10 minutes incubation in the dark on a shaker, signals were measured in a luminometer using 1 second integration time.Results for certain monoclonal antibodies are shown in Figures 12A-12D. Calculated IC50 and IC90 values (ng/ml) are shown below each graph.

EXAMPLE 11 Binding of Antibodies to RBD using Octet Binding affinity and avidity of antibodies S2X193, S2X195, S2X219, S2X244, S2X246, S2X256, S2X269, and S2X278 for SARS-CoV-2 RBD was measured by Octet. Antibody was loaded on Protein A pins at 2.7 ug/ml. SARS-CoV-2 RBD was loaded for 5 minutes at 6 ug/ml, 1.5 ug/ml, or 0.4 ug/ml. Dissociation was measured for 7 minutes. Results are shown in Figures 14A-14H. In each graph, the vertical dashed line indicates the start of the dissociation phase.Binding affinity and avidity of antibodies S2X193, S2X195, S2X219, S2X244, S2X246, S2X256, S2X269, and S2X278, along with four comparator antibodies, to SARS-C0V-1 RBD was also measured by Octet. Antibody was loaded on Protein A 291 WO 2021/226560 PCT/US2021/031442 pins at 2.7 ug/ml. SARS-CoV-1 RBD was loaded for 5 minutes at 6 ug/ml. Dissociation was measured for 7 minutes. Results are shown in Figure 15. In each graph, the vertical dashed line indicates the start of the dissociation phase.

EXAMPLE 12 Quantitative Epitope-Specific Serology of SARS-CoV-2 Spike Protein SARS-CoV-2 Spike protein antibody binding was analyzed by antibody competition assays, cryo-EM data, and crystallography data. From this analysis, Spike RBD antigenic Sites la, lb, Ic, Id, II, and IV were identified. A map showing these sites and antibodies that bind within each site is shown in Figure 24.

EXAMPLE 13 Binding of S2E12 Antibodies to SARS-CoV-2 RBD Binding of S2E12 and S2E12 variant antibodies to SARS-CoV-2 RBD was measured by surface plasmon resonance (SPR). SPR experiments were carried out with a Biacore T200 instrument using a single-cycle kinetics approach. Antibodies were captured on the surface and increasing concentrations of purified SARS-CoV-2 RBD were injected. Association and dissociation kinetics were monitored and fit to a binding model to determine affinity.Results are shown in Tables 23 and 24. Antibody "S2E12-11" was obtained from the supernatant of CHO cells transformed to express S2E12 antibody. Antibody S2E12 WT (VH of SEQ ID NO.:399, VL of SEQ ID NO.:403) was generated using purified antibody produced in transformed HEK cells. KD/KD WT lists the KD value for the indicated antibody divided by the KD value for S2E12 WT. KD WT/KD lists the KD value for S2E12 WT divided by the KD value for the indicated antibody. Blank cells indicate that no binding was measured using this assay. 292 WO 2021/226560 PCT/US2021/031442 Table 23.

Antibody Ka (1/Ms) Kd (1/s) KD (M) KD/KD WT S2E12 WT 1.98E+06 0.002335 1.18E-09 1.0S2E12-11 2.42E+06 0.003174 1.31E-09 1.1409 11 4 v5 2.68E+06 0.03576 1.33E-08 0.098409 11 4 v3 1.18E+06 0.01527 1.30E-08 0.100409 11 4 v? 1.86E+06 0.009129 4.90E-09 0.267409 11 4 v8409 11 4 v9409 11 4 vlO409 11 4 vll Table 24.

Antibody KD (M) [first phase] KD WT/KD S2E12 WT 2.28E-09 1.00409 11 4 v4 2.59E-08 0.09409 11 4 v2 7.88E-10 2.89409 11 4 v6 2.04E-08 0.11409 11 4 vl2 4.53E-08 0.05409 11 4 v!3 5.14E-08 0.04 EXAMPLE 14 ACE2-Independent Mechanism of SARS-CoV-2 Neutralization by Antibodies Mechanisms of antibody neutralization of SARS-CoV-2 infection wereinvestigated. In the following experiments, unless otherwise indicated, S309 antibody (VH of SEQ ID NO.: 139, VL of SEQ ID NO.: 143) was expressed as recombinant IgGl 293 WO 2021/226560 PCT/US2021/031442 with M428L and N434S mutations. The effect of ACE2 overexpression on S3antibody neutralization of infection was investigated. Vero E6 or Vero E6-TMPRSScells were infected with SARS-CoV-2 (isolate USA-WA1/2020) at MOI 0.01 in the presence of S309 (10 ug/ml). Cells were fixed 24h post infection, viral nucleocapsid protein was immunostained and quantified. Nucleocapsid staining was effectively absent in antibody-treated cells. S309 had an IC50 (ng/mL) in Vero E6 cells of 65 and in Vero E6-TMPRSS2 of 91 (data not shown).A panel of seven cell lines (HeLa, 293T (wt), Vero E6, Huh?, 293T ACE2, MRC 5-ACE2-TMPRSS2, A549-ACE2-TMPRSS2 clone 5, A549-ACE2-TMPRSSclone 10) were infected with SARS-C0V-2-Nluc or VSV pseudotyped with the SARS- C0V-2 spike protein in the presence of S309. Luciferase signal was quantified 24h post infection. S309 maximum neutralization values were as shown in Table 25.

Table 25. Maximum Neutralization Values of S309 Cell Type Virus/Pseudotype SARS-CoV-2-Nluc VSV Pseudotype Vero E6 >99% >99%Vero E6-TMPRSS2 >99% 96%Huh? 98% 78%293 T ACE2 26% 34%MRC5-ACE2-TMPRSS2 87% 45%A549-ACE2-TMPRSS2 clone 5 89% 65%A549-ACE2-TMPRSS2 clone 10 81% 42% S2E12 neutralization data are shown in Figure 53 (SARS-C0V-2-Nluc) and Figure 54 (pseudotyped VSV). Notably, S2E12 showed comparable neutralizing activity on all target cells.Binding of purified, fluorescently-labeled SARS-CoV-2 spike protein binding to these cell lines was quantified by flow cytometry. HeLa and 239T WT cells had he lowest MFIs, followed by Huh? and VeroE6 cells. 293T ACE2 cells (highest), MRC 5- 294 WO 2021/226560 PCT/US2021/031442 ACE2-TMPRSS2 (third-highest), A549-ACE2-TMPRSS2 clone 5 (fourth-highest), and A549-ACE2-TMPRSS2 clone 10 (second-highest) had higher MFIs. Correlation analysis between spike binding maximum neutralization potential of S309 was determined; S309 Spearman correlation values were: r = -0.94 for both viral models, p = 0.017. See Figure 55.To further characterize SARS-C0V-2-susceptible cell lines, the seven cell lines described above were incubated with purified, fluorescently-labeled SARS-C0V-spike protein or RED protein and protein binding was quantified by flow cytometry. In descending order of MFI, the cell lines were: A549-ACE2-TMPRSS2 clone 10; 293T ACE2; MRC 5-ACE2-TMPRSS2; A549-ACE2-TMPRSS2 clone 5; Vero E6; Huh7; 293T (wt); and HeLa.Selected lectins and published receptor candidates were screened using HEK293T cells infected with SARS-C0V-2 VSV pseudoviruses. ACE2, DC-SIGN, L- SIGN, and SIGLEC-1 gave the highest signals. ACE2 provided a signal of approximately 105 relative luminescence units (RLUs), and DC-SIGN, SIGLEC-1, and L-SIGN had signals of approximately 104RLUs. All other lectins/candidates tested gave signals of approximately 102 - 103 RLUs.HEK 293T, HeLa and MRC5 cells were transiently transduced to overexpress DC-SIGN, L-SIGN, SIGLEC1 or ACE2 and infected with SARS-C0V-2 VSV pseudoviruses. Uninfected cells and untransduced cells were included as controls. In HEK293T cells, ACE2, DC-SIGN, SIGLEC-1, and L-SIGN all provided substantial increases in infection. In HeLa and MRC5 cells, only ACE2 increased infection.Stable HEK293T cell lines overexpressing DC-SIGN, L-SIGN, SIGLEC-1 or ACE2 were infected with authentic SARS-C0V-2 (MOI 0.1), fixed and immunostained at 24 hours for the SARS-C0V-2 nucleoprotein. Wild-type cells (infected and uninfected) were used as controls. Increased staining was observed in cells overexpressing DC-SIGN, L-SIGN, or SIGLEC-1, and staining was significantly increased in cells overexpressing ACE2.Stable cell lines were infected with SARS-C0V-2-Nluc and luciferase levels were quantified at 24 hours. In ascending order of RLUs: uninfected (approx. 102-103 295 WO 2021/226560 PCT/US2021/031442 RLUs); parental 293T (approx. 104RLUs); DC-SIGN (approx. 105RLUs); L-SIGN (approx. 105RLUs); SIGLEC-1 (approx. 105-106RLUs); ACE2 (>107RLUs).Stable cell lines were incubated with different concentration of anti-SIGLECl mAb (clone 7-239) and infected with SARS-C0V-2-Nluc. Infection as a percentage of untreated cells remained near to exceeded 100% in 293T cells expressing DC-SIGN, L- SIGN, or ACE2, but dropped to below 50% (0.2 ug/mL anti-SIGLEC) to close to 0 (ug/mL or 5 ug/mL anti-SIGLEC) in 293T cells expressing SIGLEC-1.Single cell expression levels of selected potential SARS-CoV-2 (co)receptor candidates were determined in different lung cell types derived from the Human Lung Cell Atlas (nature.com/articles/s41586-020-2922-4 ). DC-SIGN, L-SIGN and SIGLEC- are expressed in a variety of cell types in the lung at levels similar to or even higher than ACE2.Binding of antibodies targeting DC-/L-SIGN, DC-SIGN, SIGLEC 1 or ACE2 on HEK293T cells stably over-expressing the respective attachment receptor was analyzed by flow cytometry and immunofluorescence analysis. HEK 293T cells over-expressing the respective attachment receptors were infected with VSV pseudotyped with SARS- COV-2 wildtype spike or spike bearing mutations of the BL 1.7 lineage. Luminescence was analyzed one day post infection. Infection was increased in cells expressing the attachment receptors. Infection by VSV pseudotyped with either spike was similar for each test group. Cells expressing ACE2 gave the highest luminescence signal.Vero E6 cells, in vitro differentiated moDCs or PBMCs were infected with SARS-CoV-2 at MOI 0.01. At 24h post infection, cells were fixed, immunostained for viral nucleocapsid protein and infected cells were quantified. Only VeroE6 cells showed infection (approximately 7% of cells). Supernatant of the infected cells was taken at 24, 48 and 72h and infectious viral titer was quantified by FEU assay on Vero E6 cells.Major cell types with detectable SARS-CoV-2 genome in bronchoalveolar lavage fluid (BALF) and sputum of severe COVID-19 patients were assessed. A t-SNE plot was generated, and the count of each SARS-CoV-2+ cell type was determined (total n=3,085 cells from 8 subjects in Ren et al. Cell 2021). Cell types were T, NK, 296 WO 2021/226560 PCT/US2021/031442 plasma, neutrophil, macrophage, ciliated, squamous, and secretory. Expression of ACE2, DC-SIGN, L-SIGN, SIGLEC-1, and combinations of these was assessed for each cell type. Figures 65-66.ACE2, DC-SIGN (CD209), L-SIGN (CLEC4M), SIGLEC1 transcript counts were correlated with SARS-CoV-2 RNA counts in macrophages and in secretory cells. Correlation was based on counts (before log transformation), from Ren et al. Cell 2021.Representative data showing expression of receptors in stable HEK293T cell lines are shown in Figure 41.Representative data showing the ability of VSV pseudovirus expressing SARS- C0V-2 S protein with luciferase reporter to infect the HEK293T cells was assessed using a luminescence assay are shown in Figure 42 (see also Figure 57); expression of DC-SIGN or L-SIGN increased pseudovirus infection levels by over 10-fold compared to infection of WT HEK293T cells, and expression of ACE2 increased pseudovirus infection levels by over 100-fold compared to infection of WT HEK293T cells.Neutralizing activity of mAb S309 against the VSV pseudovirus was assessed in the engineered HEK293T cells. Data are shown in Figure 43; S309 fully neutralized infection via DC-SIGN and L-SIGN, and to a lesser extent, ACE2.The ability of live SARS-CoV-2 with luciferase reporter to infect the HEK293T cells was examined using a luminescence assay. Data are shown in Figure 44; expression of DC-SIGN or L-SIGN increased live virus infection levels by over 3-fold compared to infection of WT HEK293T cells, and expression of ACE2 increased live virus infection levels by over 100-fold compared to infection of WT HEK293T cells. See also Figure 58, showing infection as determined by staining for SARS-CoV-nucleoprotein.Neutralizing activity of S309 against the VSV pseudovirus was assessed in the engineered HEK293T cells. Data are shown in Figure 45; S309 fully neutralized infection via DC-SIGN and L-SIGN, and neutralized infection via ACE2 to a lesser extent.Experiments were performed to investigate whether S309 or S2E12 antibody can neutralize entry of SARS-CoV-2 via SIGLEC-1. In the following experiments, 297 WO 2021/226560 PCT/US2021/031442 S309 antibody (VH of SEQ ID NO.:139, VL of SEQ ID NO.:143) and S2E12 antibody (VH of SEQ ID NO.:399, VL of SEQ ID NO.:403) were expressed as recombinant IgGl with M428L and N434S mutations. Briefly, stable cell HEK293T lines were generated as described above to overexpress DC-SIGN/L-SIGN, DC-SIGN, SIGLEC-1, or ACE2. Expression data are shown in Figure 46. As shown in Figure 47, expression of DC-SIGN, L-SIGN, or SIGLEC increased live virus infection levels by over 10-fold compared to infection of WT HEK293T cells, and expression of ACE2 increased pseudovirus infection levels by over 100-fold compared to infection of WT HEK293T cells. As shown in Figure 48, S309 fully neutralized infection via DC-SIGN, L-SIGN, and SIGLEC-1. As shown in Figure 49, S2E12 fully neutralized infection via SIGLEC- and ACE2.Expression of DC-SIGN (CD209) and other cell surface receptor proteins including SIGLEC-1 and other SIGLECs was determined on a variety of cell types. Data are summarized in Figures 50A and SOB.Further experiments were performed to investigate the function(s) of DC-SIGN, L-SIGN, and SIGLEC-1 in SARS-C0V-2 infection. In one set of experiments, HEK293T cells stably expressing DC-SIGN, L-SIGN, SIGLEC-1 or ACE2 were infected with live SARS-C0V-2 Nluc at three different multiplicities of infection (MOI): 0.01, 0.1, and 1). Infection was determined using relative luminescence units and compared to infection in HEK293T cells (parental). Data are shown in Figure 51. At the lowest MOI tested, an increase of infection in cells expressing DC-SIGN, L- SIGN, or SIGLEC was observed. At the highest MOI tested, infection was not further increased versus parental by expression of DC-SIGN, L-SIGN, or SIGLEC. These data indicate that the parental 293T cells are susceptible to infection by SARS-C0V-2 and L- SIGN, DC-SIGN, and SIGLEC-1 enhance infection levels but do not function as primary receptors for infection.In another set of experiments, 293T cells, HeLa cells, and MRC5 cells were transiently transduced with lentivirus encoding DC-SIGN, L-SIGN, SIGLEC-1 or ACE2 and infected with VSV pseudovirus three days after transduction. Data are shown in Figure 52. While the 293T cells showed a low level of susceptibility 298 WO 2021/226560 PCT/US2021/031442 (compare uninfected with untransduced), HeLa and MRC5 cells were completely refractory to the virus. The low level of infection in 293T cells can be increased by expression of L-SIGN, DC-SIGN, or SIGLEC-1, consistent with a role for these proteins as as attachment factors. The HeLa and MRC5 cells remained refractory to infection even after expression of L-SIGN, DC-SIGN, or SIGLEC-1, and only become susceptible after expression of ACE2. These data indicate that L-SIGN, DC-SIGN, and SIGLEC-1 are not primary receptors for SARS-CoV-2.Trans-infection, cell-cell fusion, and further neutralization of infection assays were performed. Figure 71 shows neutralization on Vero E6 cells using antibodies S309, S2E12, and S2X333. Figure 72 shows neutralization on Vero E6-TMPRSScells using the same antibodies. Figures 75 and 76 show neutralization of infection by these antibodies on various cell types. Figures 77-80 show results from cell-cell fusion and fusion inhibition assays. Figures 81-84 show neutralization of infection by antibodies on stable HEK293T cell line overexpressing ACE2, SIGLEC1, DC-SIGN, or L-SIGN.

EXAMPLE 15 In vivo Efficacy of S309 Antibody and the Combination of S309 and S2E12 Antibodies The efficacy of S309 and the combination of S309 and S2E12 was investigated in Syrian hamsters. This animal model represents to-date the most relevant model of SARS-CoV-2 infection that did not require in vivo over-expression of ACE2 to support productive infection and disease. Prophylactic administration of S309 induced dose- dependent protection against SARS-CoV-2 infection and tissue damage in hamsters, as demonstrated by the viral RNA levels, the viral load, and the histopathological score in the lungs (Fig. 56A, left column). These data indicate that poor and incomplete neutralization of entry by S309 in vitro when using ACE2 over-expressing cells did not compromise in vivo efficacy of non-RBM mAbs. Similar results were obtaining using a combination of S309 and S2E12 antibodies (Fig. 56A, right column). 299 WO 2021/226560 PCT/US2021/031442 S309 carrying the N297A mutation has a reduced capacity to trigger effector functions as a consequence of diminished engagement to Fey receptors. This was further confirmed by the reduced binding of S309-N297A variant to hamster monocytes in the spleen. The in vivo efficacy measured with the N297A mAb is similar or justslightly inferior to the wt S309, suggesting that neutralizing capacity of the mAb is prevailing upon its effector function capacity in these conditions. The serum concentration of S309 required to reduce the viral RNA in the lung by 90% was ug/ml (Figure 56B, left column). Similar results were obtained using a combination of S309 and S2E12 antibodies (Figure 56B, right column). 300 WO 2021/226560 PCT/US2021/031442 EXAMPLE 16 FURTHER IN vivo Studies using S2E12-V2 Antibody A preclinical study using non-human primates was conducted to assess pharmacokinetics and potential tissue cross-reactivity safety of S2E12-v2 (shown in Figure 99 as "S2E12") having the VH amino acid sequence of SEQ ID NO:399 and the VL amino acid sequence of SEQ ID NO:738, and comprising M428L and N434S Fc mutations. As shown in Figures 99 and 100, a single 5 mg/kg dose of S2E12-v2 MLNS had a mean T1/2 (across 3 animals) of 25.4 days. Tissue cross-reactivity studies (using CH0-C0V2-S spike, CHO-CoV2S-spike:CHO at 1:1, and CHO as controls) did not identify cross-reactive staining by S2E12-v2 in any tissue at 1.25, 0.3125, or 0.0781pg/ml.

EXAMPLE 17 Further Neutralization Studies The potential effect of known SARS-CoV-2 mutations on neutralization potency of S2E12 was investigated. The following individual mutations in SARS-CoV-2 S had less than a 3-fold decrease on neutralization of S2E12 against live SARS-CoV-2 or SARS-CoV-2 pseudovirus: N501Y; S477N; N439K; L452R; E484K; K417N; T478K; S494P; A520S; N501T; A522S; Y453F; P384L. 301 WO 2021/226560 PCT/US2021/031442 EXAMPLE 18 Materials and Methods Flow-cytometry based screening for binding to C0V S protein expressed on mammalian cells ExpiCHO cells were transfected with S protein of SARS-CoV-2. The monoclonal antibodies were then tested by flow-cytometry at 10 pg/ml for their ability to stain ExpiCHO cells expressing the S protein of SARS-CoV-2 transfectants. Transient expression of recombinant SARS-CoV-2 protein The full-length S gene of SARS-CoV-2 strain (2019-nCoV-S) isolate BetaCoV/Wuhan-Hu-1/2019 (accession number MN908947) was codon optimized for human cell expression and cloned into the phCMVl expression vector (Genlantis). Expi-CHO cells were transiently transfected with phCMVl-SARS-C0V-2-S, phCMVl- MERS-C0V-S (Londonl/2012), SARS-spike_pcDNA.3 (strain SARS) or the empty phCMVl (Mock) using Expifectamine CHO Enhancer. Two days after transfection, cells were collected, fixed, or fixed and permeabilized with saponin for immunostaining with a panel of monoclonal antibodies reactive to SARS-C0V Receptor Binding Domain (RBD). An Alexa647-labelled secondary antibody anti-human IgG Fc was used for detection. Binding of antibodies to transfected cells was analyzed by flow- cytometry using a ZES Cell Analyzer (Biorard) and FlowJo software (TreeStar). Positive binding was defined by differential staining of CoV-S-transfectants versus mock-transfectants. Competition experiments using Octet (BLI, biolayer interferometry) Unless otherwise indicated herein, anti-His sensors (BIOSENSOR ANTI- PENTA-HIS (HISIK)) were used to immobilize the SI subunit protein of SARS-C0V (Sino Biological Europe GmbH). Sensors were hydrated for 10 min with Kinetics Buffer (KB; 0.01% endotoxin-free BSA, 0.002A Tween-20, 0.005% NaN3 in PBS). SARS-C0V SI subunit protein was then loaded for 8 min at a concentration of 10 pg/ml in KB. Antibodies were associated for 6 min at 15 pg/ml for full length mAbs nCoV-and nCov-6 mAbs or 5 pg/ml for Fab nC0V-4, and in a subsequent experiment 302 WO 2021/226560 PCT/US2021/031442 compnsing nC0V-l all at 10 pg/ml. Competing antibodies were then associated at the same concentration for additional 6 mins. Competition experiments using Octet (BLI, biolayer interferometry) For ACE2 competition experiments, ACE2-His (Bio-Techne AG) was loaded for 30 minutes at 5 ug/ml in KB onto anti-HIS (HIS2) biosensors (Molecular Devices- ForteBio). SARS-CoV- RBD-rabbitFe or SARS-CoV-2 RBD-mouseFc (Sino Biological Europe GmbH) at 1 ug/ml was associated for 15 minutes, after a preincubation with or without antibody (30 ug/ml, 30 minutes). Dissociation was monitored for 5 minutes. Affinity determination using Octet (BLI, biolayer interferometry) For Kd determination of full-length antibodies, protein A biosensors (Pall ForteBio) were used to immobilize recombinant antibodies at 2.7 ug/ml for 1 minute, after a hydration step for 10 minutes with Kinetics Buffer. Association curves were recorded for 5min by incubating the antibody-coated sensors with different concentration of SARS-CoV-1 RBD (Sino Biological) or SARS-CoV-2 RBD (produced in house; residues 331-550 of spike from BetaCoV/Wuhan-Hu-1/2019, accession number MN908947). Highest RBD concentration tested was lOug/ml, then 1:2.serially diluted. Dissociation was recorded for 9min by moving the sensors to wells containing KB. Kd values were calculated using a global fit model (Octet). Octet Red96 (ForteBio) equipment was used.For Kd determination of full-length antibodies compared to Fab fragments, His- tagged RBD of SARS-CoV-1 or SARS-CoV-2 were loaded at 3 ug/ml in KB for minutes onto anti-HIS (HIS2) biosensors (Molecular Devices, ForteBio). Association of full-length antibody and Fab was performed in KB at 15 ug/ml and 5 ug/ml respectively for 5 minutes. Dissociation in KB was measured for lOmin. ELISA binding The reactivities of mAbs with SARS-CoV-2 Spike SI Subunit Protein (strain WH20) protein were determined by enzyme-linked immunosorbent assays (ELISA). Briefly, 96-well plates were coated with 3 ug/ml of recombinant SARS-CoV-2 Spike SI Subunit Protein (Sino. Biological). Wells were washed and blocked with 303 WO 2021/226560 PCT/US2021/031442 PBS+1%BSA for 1 h at room temperature and were then incubated with serially diluted mAbs for 1 h at room temperature. Bound mAbs were detected by incubating alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotechnology: 2040-04) for h at room temperature and were developed by 1 mg/ml p-nitrophenylphosphate substrate in 0.1 M glycine buffer (pH 10.4) for 30 min at room temperature. The optical density (OD) values were measured at a wavelength of 405 nm in an ELISA reader (Powerwave 340/96 spectrophotometer, BioTek). Neutralization assay Unless otherwise indicated, Murine leukemia virus (MLV) pseudotyped with SARS-CoV-2 Spike protein (SARS-C0V-2pp) or SARS-C0V-1 Spike protein (SARS- CoV-lpp) were used. DBT cells stably transfected with ACE2 (DBT-ACE2) were used as target cells. SARS-C0V-2pp or SARS-CoV-1pp was activated with trypsin TPCK at lOug/ml. Activated SARS-C0V-2pp or SARS-CoV-lpp was added to a dilution series of antibodies (starting 50ug/ml final concentration per antibody, 3-fold dilution). DBT- ACE2 cells were added to the antibody-virus mixtures and incubated for 48h. Luminescence was measured after aspirating cell culture supernatant and adding steady- GLO substrate (Promega).Unless otherwise indicated, pseudoparticle neutralization assays use a VSV- based luciferase reporter pseudotyping system (Kerafast). VSV pseudoparticles and antibody are mixed in DMEM and allowed to incubate for 30 minutes at 37C. The infection mixture is then allowed to incubate with Vero E6 cells for Ih at 37C, followed by the addition of DMEM with Pen-Strep and 10% FBS (infection mixture is not removed). The cells are incubated at 37C for 18-24 hours. Luciferase is measured using an Ensight Plate Reader (Perkin Elmer) after the addition of Bio-Gio reagent (Promega). SPR single-cycle kinetics SPR experiments were carried out with a Biacore T200 instrument using a single-cycle kinetics approach. S309 IgG was captured on the surface and increasing concentrations of purified SARS-CoV-2 RBD, either glycosylated or deglycosylated, 304 WO 2021/226560 PCT/US2021/031442 were injected. Association and dissociation kinetics were monitored and nt to a binding model to determine affinity. Expression of recombinant antibodies Recombinant antibodies were expressed in ExpiCHO cells transiently co- transfected with plasmids expressing the heavy and light chain as previously described. (Stettler et al. (2016) Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection. Science, 353(6301), 823-826) Monoclonal antibodies S303, S304, S306, S309, S310, and S315 were expressed as rlgG-LS antibodies. The LS mutation confers a longer half-life in vivo. (Zalevsky et al. (2010) Enhanced antibody half-life improves in vivo activity. Nature Biotechnology, 28(2), 157-159) Sequence alignment SARS-C0V-2 genomics sequences were downloaded from GISAID on March 29th 2020, using the "complete (>29,000 bp)" and "low coverage exclusion " filters. Bat and pangolin sequences were removed to yield human-only sequences. The spike ORF was localized by performing reference protein (YP_009724390. !)-genome alignments with GeneWise2. Incomplete matches and indel-containing ORFs were rescued and included in downstream analysis. Nucleotide sequences were translated in silica using seqkit. Sequences with more than 10% undetermined aminoacids (due to N basecalls) were removed. Multiple sequence alignment was performed using MAFFT. Variants were determined by comparison of aligned sequences (n=2,229) to the reference sequence using the R/Bioconductor package Biostrings. A similar strategy was used to extract and translate spike protein sequences from SARS-C0V genomes sourced from ViPR (search criteria: SARS-related coronavirus, full-length genomes, human host, deposited before December 2019 to exclude SARS-C0V-2, n=53). Sourced SARS-C0V genome sequences comprised all the major published strains, such as Urbani, Tor2, TW1, P2, Frankfurtl, among others. Pangolin sequences as shown by Tsan-Yuk Lam et al were sourced from GISAID. Bat sequences from the three clades of Sarbecoviruses as shown by Lu et al (Lancet 2020) were sourced from Genbank. Civet and racoon dog sequences were similarly sourced from Genbank.Generation of stable overexpression cell lines 305 WO 2021/226560 PCT/US2021/031442 Lentiviruses were generated by co-transfection of Lenti-X 293T cells (Takara) with lentiviral expression plasmids encoding DC-SIGN (CD209), L-SIGN (CLEC4M), SIGLEC1, TMPRSS2 or ACE2 (all obtained from Genecopoeia) and the respective lentiviral helper plasmids. Forty-eight hours post transfection, lentivirus in the supernatant was harvested and concentrated by ultracentrifugation for 2 h at 20,0rpm. Lenti-X 293T (Takara), Vero E6 (ATCC), MRC5 (Sigma-Aldrich), A549 (ATCC) were transduced in the presence of 6 ug/mL polybrene (Millipore) for 24 h. Cell lines overexpressing two transgenes were transduced subsequently. Selection with puromycin and/or blasticidin (Gibco) was started two days after transduction and selection reagent was kept in the growth medium for all subsequent culturing. Single cell clones were derived from the A549-ACE2-TMPRSS2 cell line, all other cell lines represent cell pools.SARS-CoV-2 neutralizationVero E6 or Vero E6-TMPRSS2 cells cultured in DMEM supplemented with 10% FBS (VWR) and lx Penicillin/Streptomycin (Thermo Fisher Scientific) were seeded in black 96-well plates at 20,000 cells/well. Serial 1:4 dilutions of the monoclonal antibodies were incubated with 200 pfu of SARS-CoV-2 (isolate USA- WA1/2020, passage 3, passaged in Vero E6 cells) for 30 min at 37°C in a BSL-facility. Cell supernatant was removed and the virus-antibody mixture was added to the cells. 24 h post infection, cells were fixed with 4% paraformaldehyde for 30 min, followed by two PBS (pH 7.4) washes and permeabilization with 0.25% Triton X-1in PBS for 30 min. After blocking in 5% milk powder/PBS for 30 min, cells were incubated with a primary antibody targeting SARS-CoV-2 nucleocapsid protein (Sino Biological, cat. 40143-R001) at a 1:2000 dilution for Ih. After washing and incubation with a secondary Alexa647-labeled antibody mixed with 1 ug/ml Hoechst33342 for hour, plates were imaged on an automated cell-imaging reader (Cytation 5, Biotek) and nucleocapsid-positive cells were counted using the manufacturer ’s supplied software.SARS-CoV-2-Nluc neutralizationNeutralization was determined using SARS-C0V-2-Nluc, an infectious clone of SARS-CoV-2 (based on strain 2019-nCoV/USA_WAl/2020) encoding nanoluciferase 306 WO 2021/226560 PCT/US2021/031442 in place of the viral ORF7, which demonstrates comparable growth kinetics to wild type virus (Xie et al., Nat Comm, 2020, https://doi.org/10.1038/s41467-020-19055-7 ). Cells were seeded into black-walled, clear-bottom 96-well plates at 20,000 cells/well (293T cells were seeded into poly-L-lysine-coated wells at 35,000 cells/well) and cultured overnight at 37°C. The next day, 9-point 4-fold serial dilutions of antibodies were prepared in infection media (DMEM + 10% FBS). SARS-C0V-2-Nluc was diluted in infection media at the indicated MOI, added to the antibody dilutions and incubated for min at 37°C. Media was removed from the cells, mAb-virus complexes were added, and cells were incubated at 37°C for 24 h. Media was removed from the cells, Nano- Gio luciferase substrate (Promega) was added according to the manufacturer ’s recommendations, incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).SARS-CoV-2 pseudotyped VSV production and neutralizationTo generate SARS-CoV-2 pseudotyped vesicular stomatitis virus, Lenti-X 293T cells (Takara) were seeded in 10-cm dishes for 80% next day confluency. The next day, cells were transfected with a plasmid encoding for SARS-CoV-2 S-glycoprotein (YP_009724390.1) harboring a C-terminal 19 aa truncation using TransIT-Lenti (Mirus Bio) according to the manufacturer ’s instructions. One day post-transfection, cells were infected with VSV(G*AG-luciferase) (Kerafast) at an MOI of 3 infectious units/cell. Viral inoculum was washed off after one hour and cells were incubated for another day at 37°C. The cell supernatant containing SARS-CoV-2 pseudotyped VSV was collected at day 2 post-transfection, centrifuged at 1000 x g for 5 minutes to remove cellular debris, aliquoted, and frozen at -80°C.For viral neutralization, Cells were seeded into black-walled, clear-bottom 96- well plates at 20,000 cells/well (293T cells were seeded into poly-L-lysine-coated wells at 35,000 cells/well) and cultured overnight at 37°C. The next day, 9-point 4-fold serial dilutions of antibodies were prepared in media. SARS-CoV-2 pseudotyped VSV was diluted 1:30 in media in the presence of 100 ng/mL anti-VSV-G antibody (clone 8G5F11, Absolute Antibody) and added 1:1 to each antibody dilution. Virus:antibody mixtures were incubated for 1 hour at 37°C. Media was removed from the cells and 307 WO 2021/226560 PCT/US2021/031442 pL of virus :antibody mixtures were added to the cells. One hour post-infection, 100 pL of media was added to all wells and incubated for 17-20 hours at 37°C. Media was removed and 50 pL of Bio-Gio reagent (Promega) was added to each well. The plate was shaken on a plate shaker at 300 RPM at room temperature for 15 minutes and RLUs were read on an EnSight plate reader (Perkin-Elmer).Transfection-based attachment receptor screenLenti-X 293T cells (Takara) were transfected with plasmids encoding the following receptor candidates (all purchased from Genecopoeia): ACE2 (NM_021804), DC-SIGN (NM_021155), L-SIGN (BC110614), LGALS3 (NM_002306), SIGLEC(NM_023068), SIGLEC3 (XM_057602), SIGLEC9 (BC035365), SIGLEC(NM_033130), MGL (NM_182906), MINGLE (NM_014358), CD147 (NM_198589), ASGR1 (NM_001671.4), ASGR2 (NM_080913), NRP1 (NM_003873). One day post transfection, cells were infected with SARS-C0V-2 pseudotyped VSV at 1:20 dilution in the presence of 100 ng/mL anti-VSV-G antibody (clone 8G5F11, Absolute Antibody) at 37°C. One hour post-infection, 100 pL of media was added to all wells and incubated for 17-20 hours at 37°C. Media was removed and 50 pL of Bio-Gio reagent (Promega) was added to each well. The plate was shaken on a plate shaker at 300 RPM at room temperature for 15 minutes and RLUs were read on an EnSight plate reader (Perkin-Elmer).Trans-infectionParental HeLa cells or HeLa cells stably expressing DC-SIGN, L-SIGN or SIGLEC1 were seeded at 5,000 cells per well in black-walled clear-bottom 96-well plates. One day later, cells reached about 50% confluency and were inoculated with SARS-C0V-2 pseudotyped VSV at 1:10 dilution in the presence of 100 ng/mL anti- VSV-G antibody (clone 8G5F11, Absolute Antibody) at 37°C for 2 h. For antibody- mediated inhibition of trans-infection, cells were pre-incubated with 10 ug/mL anti- SIGLEC1 antibody (Biolegend, clone 7-239) for 30 min. After 2 h inoculation, cells were washed four times with complete medium and 10,000 VeroE6-TMPRSS2 cells per well were added and incubated 17-20 h at 37°C for trans-infection. Media was removed and 50 pL of Bio-Gio reagent (Promega) was added to each well. The plate was shaken 308 WO 2021/226560 PCT/US2021/031442 on a plate shaker at 300 RPM at room temperature for 15 minutes and RLUs were read on an EnSight plate reader (Perkin-Elmer).Cell-cell fusion of CHO-S cellsCHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day, different concentrations of mAbs and nuclei marker Hoechst (final dilution 1:1000) were added to the cells and incubated for additional 24h hours. Fusion degree was established using the Cytation 5 Imager (BioTek) and an object detection protocol was used to detect nuclei as objects and measure their size. The nuclei of fused cells (i.e., syncytia) are found aggregated at the center of the syncitia and are recognized as a unique large object that is gated according to its size. The area of the objects in fused cells divided by the total area of all the object multiplied by 1provides the percentage of fused cellsImmunofluorescence analysisHEK 293T cells were seeded onto poly-D-Lysine-coated 96-well plates (Sigma- Aldrich) and fixed 24 h after seeding with 4% paraformaldehyde for 30 min, followed by two PBS (pH 7.4) washes and permeabilization with 0.25% Triton X-100 in PBS for min. Cells were incubated with primary antibodies anti-DC-SIGN/L-SIGN (Biolegend, cat. 845002, 1:500 dilution), anti-DC-SIGN (Cell Signaling, cat. 13193S, 1:500 dilution), anti-SIGLECl (Biolegend, cat. 346002, 1:500 dilution) or anti-ACE(R&D Systems, cat. AF933, 1:200 dilution) diluted in 3% milk powder/PBS for 2 h at room temperature. After washing and incubation with a secondary Alexa647-labeled antibody mixed with 1 ug/ml Hoechst33342 for 1 hour, plates were imaged on an inverted fluorescence microscope (Echo Revolve).ACE2/TMPRSS2 RT-qPCRRNA was extracted from the cells using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer ’s protocol. RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) according to the manufacturer ’s instructions. Intracellular levels of ACE(Forward Primer: CAAGAGCAAACGGTTGAACAC, Reverse Primer: 309 WO 2021/226560 PCT/US2021/031442 CCAGAGCCTCTCATTGTAGTCT), HPRT (Forward Primer: CCTGGCGTCGTGATTAGTG, Reverse Primer: ACACCCTTTCCAAATCCTCAG), and TMPRSS2 (Forward Primer: CAAGTGCTCCRACTCTGGGAT, Reverse Primer: AACACACCGRTTCTCGTCCTC) were quantified using the Luna Universal qPCR Master Mix (New England Biolabs) according to the manufacturer ’s protocol. Levels of ACE2 and TMPRSS2 were normalized to HPRT. Hela cells were used as the reference sample. All qPCRs were run on a QuantStudio 3 Real-Time PCR System (Applied Biosystems).SARS2 D614G Spike Production and biotinylationPrefusion-stabilized SARS2 D614G spike (comprising amino acid sequence Q14toK1211) with a C-terminal TEV cleavage site, T4 bacteriophage fibritin foldon, 8x His-, Avi- and EPEA-tag was transfected into HEK293 Freestyle cells, using 293fectin as a transfection reagent. Cells were left to produce protein for three days at 37°C. Afterwards, supernatant was harvested by centrifuging cells for 30 minutes at 5xg, followed by another spin for 30 minutes at 4000 xg. Cell culture supernatant was filtered through a 0.2 um filter and loaded onto a 5 mL C-tag affinity matrix column, pre-equilibrated with 50 mM Tris pH 8 and 200 mM NaCl. SARS2 D614G spike was eluted, using 10 column volumes of 100 mM Tris, 200 mM NaCl and 3.8 mM SEPEA peptide. Elution peak was concentrated and injected on a Superose 6 increase 10/3GL gel filtration column, using 50 mM Tris pH 8 and 200 mM NaCl as a running buffer. SEC fractions corresponding to monodisperse SARS2 D614G spike were collected and flash frozen in liquid nitrogen for storage at -80°C. Purified SARSD614G spike protein was biotinylated using BirA500 biotinylation kit from Avidity. To ug of spike protein, 5 ug of BirA, and 11 uL of BiomixA and BiomixB was added. Final spike protein concentration during the biotinylation reaction was ~1 uM. The reaction was left to proceed for 16 hours at 4°C. Then, protein was desalted using two Zeba spin columns pre-equilibrated with lx PBS pH 7.4.Flow cytometry analysis for DC-SIGN, L-SIGN, SIGLEC1 and ACE-2HEK 293T cells expressing DC-SIGN, L-SIGN, SIGLEC1 or ACE2 were resuspended at 4xl0 6 cells/mL and 100 pL per well were seeded onto V-bottom 96-well 310 WO 2021/226560 PCT/US2021/031442 plates (Corning, 3894). The plate was centrifuged at 2,000 rpm for 5 minutes and washed with PBS (pH 7.4). The cells were resuspended in 200 pL of PBS containing Ghost violet 510 viability dye (Cell Signaling, cat. 13-0870-T100, 1:1,000 dilution), incubated for 15 minutes on ice and then washed. The cells were resuspended in 100 pL of FACS buffer prepared with 0.5% BSA (Sigma-Aldrich) in PBS containing the primary antibodies at a 1:100 dilution: mouse anti-DC/L-SIGN (Biolegend, cat. 845002), rabbit anti-DC-SIGN (Cell Signaling, cat. 13193), mouse anti-SIGLECl (Biologend, cat. 346002) or goat anti-ACE2 (R&D Systems, cat. AF933). After 1 h incubation on ice, the cells were washed two times and resuspended in FACS buffer containing the Alexa Fluor-488-labeled secondary antibodies at a 1:200 dilution: goat anti-mouse (Invitrogen cat. Al 1001), goat anti-rabbit (Invitrogen cat. Al 1008) or donkey anti-goat (Invitrogen cat. Al 1055). After incubation for 45 min on ice, the cells were washed three times with 200pL of FACS buffer and fixed with 200pL of 4% PF A (Alfa Aesar) for 15 mins at room temperature. Cells were washed three times, resuspended in 200pL of FACS buffer and analyzed by flow cytometry using the CytoFLEX flow cytometer (Beckman Coulter).Flow cytometry of SARS-CoV-2 Spike and RED binding to cells Biotinylated SARS-CoV-2 Spike D614G protein (Spikebiotin, in-house generated) or the biotinylated SARS-CoV-2 Spike receptor-binding domain (RBDbiotin, Sino Biological, 40592-V08B) were incubated with Alexa Fluor® 6streptavidin (AF647-strep, Invitrogen, S21374) at a 1:20 ratio by volume for 20 min at room temperature. The labeled proteins were then stored at 4°C until further use. Cells were dissociated with TrpLE Express (Gibco, 12605-010) and 105 cells were transferred to each well of a 96-well V bottom plate (Coming, 3894). Cells were washed twice in flow cytometry buffer (2% FBS in PBS (w/0 Ca/Mg)) and stained with Spikebiotin- AF647-strep at a final concentration of 20 pg/ml or RBDbiotin-AF647-strep at a final concentration of 7.5 pg/ml for Ih on ice. Stained cells were washed twice with flow cytometry buffer, resuspended in 1% PF A (Electron Microscopy Sciences, 15714-S) and analyzed with the Cytoflex LX (Beckman Coulter).Recombinant expression of SARS-CoV-2-specific mAbs. 311 WO 2021/226560 PCT/US2021/031442 Human mAbs were isolated from plasma cells or memory B cells of SARS- C0V-2 immune donors, as previously described. Recombinant antibodies were expressed in ExpiCHO cells at 37°C and 8% CO2. Cells were transfected using ExpiFectamine. Transfected cells were supplemented 1 day after transfection with ExpiCHO Feed and ExpiFectamine CHO Enhancer. Cell culture supernatant was collected eight days after transfection and filtered through a 0.2 pm filter. Recombinant antibodies were affinity purified on an KTA xpress FPLC device using 5 mL HiTrapTM MabSelectTM PrismA columns followed by buffer exchange to Histidine buffer (20 mM Histidine, 8% sucrose, pH 6) using HiPrep 26/10 desalting columnsSARS-CoV-2 injection model in hamsterVirus preparationThe SARS-CoV-2 strain used in this study, BetaCov/Belgium/GHB-03021/20(EPI ISE 109 407976|2020-02-03), was recovered from a nasopharyngeal swab taken from an RT-qPCR confirmed asymptomatic patient who returned from Wuhan, China in February 2020. A close relation with the prototypic Wuhan-Hu- 1 2019-nCoV (GenBank accession 112 number MN908947.3) strain was confirmed by phylogenetic analysis. Infectious virus was isolated by serial passaging on HuH7 and Vero E6 cells; passage 6 virus was used for the study described here. The titer of the virus stock was determined by end-point dilution on Vero E6 cells by the Reed and Muench method.CellsVero E6 cells (African green monkey kidney, ATCC CRL-1586) were cultured in minimal essential medium (Gibco) supplemented with 10% fetal bovine serum (Integro), 1% L- glutamine (Gibco) and 1% bicarbonate (Gibco). End-point titrations were performed with medium containing 2% fetal bovine serum instead of 10%.SARS-CoV-2 injection model in hamstersThe hamster infection model of SARS-CoV-2 has been described before. The specific study design is shown in the schematic below. In brief, wild-type Syrian Golden hamsters (Mesocricetus auratus) were purchased from Janvier Laboratories and were housed per two in ventilated isolator cages (IsoCage N Biocontainment System, Tecniplast) with ad libitum access to food and water and cage enrichment (wood block). 312 WO 2021/226560 PCT/US2021/031442 The animals were acclimated for 4 days prior to study start. Housing conditions and experimental procedures were approved by the ethics committee of animal experimentation of KU Leuven (license P065- 2020). Female 6-8 week old hamsters were anesthetized with ketamine/xylazine/atropine and inoculated intranasally with pL containing 2x 106 TCID50 SARS-CoV-2 (day 0).Treatment regimenAnimals were prophylactically treated 48h before infection by intraperitoneal administration (i.p.) and monitored for appearance, behavior, and weight. At day 4 post infection (p.i.), hamsters were euthanized by i.p. injection of 500 pL Dolethal (2mg/mL sodium pentobarbital, Vetoquinol SA). Lungs were collected and viral RNA and infectious virus were quantified by RT-qPCR and end-point virus titration, respectively. Blood samples were collected before infection for PK analysis.SARS-CoV-2 RT-qPCRCollected lung tissues were homogenized using bead disruption (Precellys) in 350pL RLT buffer (RNeasyMinikit, Qiagen)and centrifuged (10.000 rpm, 5 min) to pellet the cell debris. RNA was extracted according to the manufacturer ’s instructions. Of 50 pL eluate, 4 pL was used as a template in RT-qPCR reactions. RT-qPCR was performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One- Step RT-qPCR kit (BioRad) with N2 primers and probes targeting the nucleocapsid. Standards of SARS-CoV-2 cDNA (IDT) were used to express viral genome copies per mg tissue or per mL serum.End-point virus titrationsLung tissues were homogenized using bead disruption (Precellys) in 350 pL minimal essential medium and centrifuged (10,000 rpm, 5min, 4°C) to pellet the cell debris. To quantify infectious SARS-CoV-2 particles, endpoint titrations were performed on confluent Vero E6 cells in 96- well plates. Viral titers were calculated by the Reed and Muench method using the Lindenbach calculator and were expressed as 50% tissue culture infectious dose (TCID50) per mg tissue. 313 WO 2021/226560 PCT/US2021/031442 HistologyFor histological examination, the lungs were fixed overnight in 4% formaldehyde and embedded in paraffin. Tissue sections (5 pm) were analyzed after staining with hematoxylin and eosin and scored blindly for lung damage by an expert pathologist. The scored parameters, to which a cumulative score of 1 to 3 was attributed, were the following: congestion, intra-alveolar hemorrhagic, apoptotic bodies in bronchus wall, necrotizing bronchiolitis, perivascular edema, bronchopneumonia, perivascular inflammation, peribronchial inflammation and vasculitis.Binding of immunocomplexes to hamster monocytesImmunocomplexes (IC) were generated by complexing S309 mAb (hamster IgG, either wt or N297A) with a biotinylated anti-idiotype fab fragment and Alexa-488- streptavidin, using a precise molar ratio (4:8:1, respectively). Pre-generated fluorescent IC were serially diluted incubated at 4°C for 3 hrs with freshly revitalized hamster splenocytes, obtained from a naive animal. Cellular binding was then evaluated by cytometry upon exclusion of dead cells and physical gating on monocyte population. Results are expressed as Alexa-488 mean florescent intensity of the entire monocyte population.Bioinformatic analysesProcessed Human Lung Cell Atlas (HLCA) data and cell-type annotations were downloaded from Github (github.com/krasnowlab/HLCA ). Processed single-cell transcriptome data and annotation of lung epithelial and immune cells from SARS- C0V-2 infected individuals were downloaded from NCBI GEO database (ID: GSE158055) and Github (github.com/zhangzlab/covid_balf ). Available sequence data from the second single-cell transcriptomics study by Liao et al. were downloaded from NCBI SRA (ID: PRJNA608742) for inspection of reads corresponding to viral RNA. The proportion of sgRNA relative to genomic RNA was estimated by counting TRS- containing reads supporting a leader-TRS junction. Criteria and methods for detection of leader-TRS junction reads were adapted from Alexandersen et al. The viral genome reference and TRS annotation was based on Wuhan-Hu- 1 NC_045512.2/MN90894749. 314

Claims (107)

WO 2021/226560 PCT/US2021/031442 CLAIMS What is claimed is:

1. An antibody, or antigen-binding fragment thereof, comprising a heavychain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein:(i) the CDRH1 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 400, 23, 33, 43, 53, 63, 75, 85, 97, 107, 120, 130, 140, 147, 160, 170, 174, 183, 190, 199, 209, 219, 229, 241, 255, 265, 275, 285, 299, 313, 323, 333, 370, 380, 390, 410, 420, 430, 435, 445, 455, 465, 475, 485, 495, 505, 515, 525, 535, 545, 555, 565, 575, 585, 595, 605, 615, 631, and 693, or a sequence variant thereof comprising one, two, or three acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(ii) the CDRH2 comprises or consists of the amino acid sequence according to anyone of SEQ ID NOs.: 401, 24, 34, 44, 54, 64, 76, 86, 98, 108, 121, 131, 141, 148, 151, 161, 171, 184, 200, 210, 220, 230, 242, 256, 266, 276, 286, 300, 314, 324, 334,352, 360, 362, 364, 366, 371, 381, 391, 411, 421, 431, 436, 446, 456, 466, 476, 486,496, 506, 516, 526, 536, 546, 556, 566, 576, 586, 596, 606, 616, 625, 632, 635, 637,639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, 661, 663, 665, 667, 669, 671,673, 675, 677, 679, 681, 683, 685, and 694, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(iii) the CDRH3 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 766, 25, 35, 45, 55, 65, 77, 87, 99, 109, 122, 132, 142, 149,162, 164, 165, 172, 176, 177, 179, 180, 185, 187, 188, 201, 211, 221, 231, 243, 257,267, 277, 287, 301, 315, 325, 335, 354, 372, 382, 392, 412, 422, 432, 437, 447, 457,467, 477, 487, 497, 507, 517, 527, 537, 547, 557, 567, 577, 587, 597, 607, 617, 627, 316 WO 2021/226560 PCT/US2021/031442 633, 695, 751, 753, 755, 757, 760, 763, 765, and 402, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(iv) the CDRL1 comprises or consists of the amino acid sequence according to anyone of SEQ ID NOs.: 404, 27,37, 47, 57, 67, 79, 89, 101, 111, 124, 134, 144,152, 155, 156, 158, 159, 166, 181, 192, 203, 213, 223, 233, 245, 259, 269, 279, 289,303, 317, 327, 337, 356, 374, 384, 394, 414, 424, 439, 449, 459, 469, 479, 489, 499,509, 519, 529, 539, 549, 559, 569, 579, 589, 599, 609, 619, 687, and 697, or a sequencevariant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid;(v) the CDRL2 comprises or consists of the amino acid sequence according to any one of SEQ ID NOs.: 405, 28, 38, 48, 58, 68, 80, 90, 102, 112, 125, 135, 145, 153, 167, 182, 193, 204, 214, 224, 234, 246, 260, 270, 280, 290, 304, 318, 328, 338, 375, 385, 395, 415, 425, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 688, and 698, or a sequence variant thereof comprising one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid; and/or(vi) the CDRL3 comprises or consists of the amino acid sequence according to anyone of SEQ ID NOs: 406, 29,39, 49, 59, 69,81,91, 103, 113, 126, 136, 146,169, 195, 197, 205, 215, 225, 235, 247, 261, 271, 281, 291, 305, 319, 329, 339, 358,376, 386, 396, 416, 426, 441, 451, 461, 471, 481, 491, 501, 511, 521, 531, 541, 551,561, 571, 581, 591, 601, 611, 621, 689, 699, 745 and 747, or a sequence variant thereofcomprising having one, two, or three amino acid substitutions, one or more of which substitutions is optionally a conservative substitution and/or is a substitution to a germline-encoded amino acid, 317 WO 2021/226560 PCT/US2021/031442 wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-C0V-2 expressed on a cell surface of a host cell and/or on a virion.

2. The antibody or antigen-binding fragment of claim 1, which is capable of neutralizing a SARS-CoV-2 infection in an in vitro model of infection and/or in an in vivo animal model of infection and/or in a human.

3. The antibody or antigen-binding fragment of any one of claims 1-2, comprising CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences of SEQ ID NOs.:(i) 400, 401, 766, and 404-406, respectively;(ii)400-402 and 404-406, respectively;(iii) 43-45 and 47-49, respectively;(iv) 53-55 and 57-59, respectively;(v) 63-65 and 67-69, respectively;(vi) 75-77 and 79-81, respectively;(vii) 85-87 and 89-91, respectively;(viii) 97-99 and 101-103, respectively;(ix) 107-109 and 111-113, respectively;(x) 120-122 and 124-126, respectively;(xi) 130-132 and 134-136, respectively;(xii) 23 or 147, any one of 24, 148 or 151, 25 or 149, any one of 27, 152, 155, 156, 158, or 159, 28 or 153, and 29, respectively;(xiii) 43 or 160, 44 or 161, any one of 45, 162, 164, or 165, 47 or 166, 48 or167, and 49 or 169, respectively;(xiv) any one of 130, 170, or 174, 130, 131, 132, 134 or 181, 135 or 182, and 136, respectively;(xv) any one of 53, 183, or 190, 54 or 184, any one of 55, 185, 187, or 188, or 192, 58 or 193, and any one of 59, 195, or 197, respectively; 318 WO 2021/226560 PCT/US2021/031442 (xvi) 199-201 and 203-205, respectively;(xvii) 209-211 and 213-215, respectively;(xviii) 219-221 and 223-225, respectively;(xix) 229-231 and 233-235, respectively;(xx) 241-243 and 245-247, respectively;(xxi) 255-257 and 259-261, respectively;(xxii) 265-267 and 269-271, respectively;(xxiii) 275-277 and 279-281, respectively;(xxiv) 285-287 and 289-291, respectively;(xxv) 299-301 and 303-305, respectively;(xxvi) 313-315 and 317-319, respectively;(xxvii) 323-325 and 327-329, respectively;(xxviii) 333-335 and 337-339, respectively;(xxix) 229, 230 or 352, 231 or 354, and 233 or 356, 234, and 235 or 358, respectively;(xxx) 313, any one of 314, 360, 362, 364, or 366, 315 and 317-319, respectively;(xxxi) 370-372 and 374-376, respectively;(xxxii) 380-382 and 384-386, respectively;(xxxiii) 390-392 and 394-396, respectively;(xxxiv)23-25 and 27-29, respectively;(xxxv) 410-412 and 414-416, respectively;(xxxvi) 420-422 and 424-426, respectively;(xxxvii) 435-437 and 439-441, respectively;(xxxviii) 445-447 and 449-451, respectively;(xxxix) 455-457 and 459-461, respectively;(xxxx) 465-467 and 469-471, respectively;(xxxxi) 475-477 and 479-481, respectively;(xxxxii) 485-487 and 489-491, respectively;(xxxxiii) 494-497 and 499-501, respectively; 319 WO 2021/226560 PCT/US2021/031442 (xxxxiv) 505-507 and 509-511, respectively;(xxxxv) 515-517 and 519-521, respectively;(xxxxvi) 525-527 and 529-531, respectively;(xxxxvii) 535-537 and 539-541, respectively;(xxxxviii) 545-547 and 549-551, respectively;(xxxxix) 555-557 and 559-561, respectively;(xxxxx) 565-567 and 569-571, respectively;(xxxxxi) 575-577 and 579-581, respectively;(xxxxxii) 585, 586 or 625, 587 or 627, and 589-591, respectively;(xxxxxiii) 595-597 and 599-601, respectively;(xxxxxiv) 605-607 and 609-611, respectively;(xxxxxv) 615-617 and 619-621, respectively;(xxxxxvi) 631, 632 or 635 or 637 or 639 or 641 or 643 or 645 or 647 or 649 or 651 or 653 or 655 or 657 or 659 or 661 or 663 or 665 or 667 or 669 or 671 or 673 or 675 or 677 or 679 or 681 or 683 or 685, 633, and 697-699, respectively;(xxxxxvii) 693-695 and 697-699, respectively;(xxxxxviii) 400, 401 and any one of 751, 753, 755, 757, or 760 and 404, 405, and any one of 745 or 747, respectively;(xxxxxxix) 585, 586, and 762 or 764 and 589-591, respectively;(xxxxxxx) 33-35 and 37-39, respectively; or(xxxxxxxi) 400, 401, 766, 404, 405 or variant of 405 comprising one, two, or three amino acid substiutions, wherein each of the one, two, or three amino acid substitutions is optionally a conservative amino acid substitution, and 406, respectively.

4. An antibody, or antigen-binding fragment thereof, comprising the CDRH1, the CDRH2, and the CDRH3 of the VH amino acid sequence set forth in SEQ ID NO. :399, and the CDRL1, the CDRL2 or a variant of the CDRL2 comprising one, two, or three amino acid substiutions, wherein each of the one, two, or three amino acid substitutions is optionally a conservative amino acid substitution, and the CDRL3 of the 320 WO 2021/226560 PCT/US2021/031442 VL amino acid sequence set forth in SEQ ID NO.:738, wherein the CDRs are according to IMGT,and wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

5. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRHI, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 766, 404, 405, and 406, respectively; (b) SEQ ID NOs. :400, 401, 769, 404, 405, and 406, respectively; (c) SEQ ID NOs. :400, 401, 770, 404, 405, and 406, respectively; (d) SEQ ID NOs. :400, 401, 771, 404, 405, and 406, respectively; (e) SEQ ID NOs. :400, 401, 772, 404, 405, and 406, respectively; (f) SEQ ID NOs.:400, 401, 773, 404, 405, and 406, respectively; (g) SEQ ID NOs. :400, 401, 766, 404, 405, and 745, respectively; (h) SEQ ID NOs. :400, 401, 769, 404, 405, and 745, respectively; (i) SEQ ID NOs. :400, 401, 770, 404, 405, and 745, respectively; (j) SEQ ID NOs.:400, 401, 771, 404, 405, and 745, respectively; (k) SEQ ID NOs. :400, 401, 772, 404, 405, and 745, respectively; (1) SEQ ID NOs.: 400, 401, 773, 405, 405, and 745, respectively; (m) SEQ ID NOs. :400, 401, 766, 404, 405, and 747, respectively; (n) SEQ ID NOs. :400, 401, 769, 404, 405, and 747, respectively; (o) SEQ ID NOs. :400, 401, 770, 404, 405, and 747, respectively; (p) SEQ ID NOs. :400, 401, 771, 404, 405, and 747, respectively; (q) SEQ ID NOs. :400, 401, 772, 404, 405, and 747, respectively; or (r) SEQ ID NOs. :400, 401, 773, 404, 405, and 747, respectively,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion. 321 WO 2021/226560 PCT/US2021/031442

6. The antibody or antigen-binding fragment thereof of claim 5, comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein CDRHI, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 766, 404, 405, and 406, respectively.

7. The antibody or antigen-binding fragment of any one of claims 4-6, comprising the amino acid sequences set forth in: (a) SEQ ID NOs. :400, 401, 402, 404, 405, and 406; (b) SEQ ID NOs. :400, 401, 751, 404, 405, and 406; (c) SEQ ID NOs.:400, 401, 753, 404, 405, and 406; (d) SEQ ID NOs.:400, 401, 755, 404, 405, and 406; (e) SEQ ID NOs. :400, 401, 757, 404, 405, and 406; (f) SEQ ID NOs. :400, 401, 760, 404, 405, and 406; (g) SEQ ID NOs.:400, 401, 402, 404, 405, and 745; (h) SEQ ID NOs.:400, 401, 751, 404, 405, and 745; (i) SEQ ID NOs.:400, 401, 753, 404, 405, and 745; (j) SEQ ID NOs.:400, 401, 755, 404, 405, and 745; (k) SEQ ID NOs.:400, 401, 757, 404, 405, and 745; (1) SEQ ID NOs.: 400, 401, 760, 404, 405, and 745; (m) SEQ ID NOs. :400, 401, 402, 404, 405, and 747; (n) SEQ ID NOs. :400, 401, 751, 404, 405, and 747; (o) SEQ ID NOs.:400, 401, 753, 404, 405, and 747; (p) SEQ ID NOs.:400, 401, 755, 404, 405, and 747; (q) SEQ ID NOs. :400, 401, 757, 404, 405, and 747; or (r) SEQ ID NOs. :400, 401, 760, 404, 405, and 747.

8. The antibody or antigen-binding fragment of any one of claims 4-7, comprising, in VH, the amino acid sequence set forth in SEQ ID NO.: 400, the amino acid sequence set forth in SEQ ID NO. :401, and the amino acid sequence set forth in SEQ ID NO. :402, and in VL, the amino acid sequence set forth in SEQ ID NO. :404, the amino acid sequence set forth in SEQ ID NO. :405, and the amino acid sequence set forth in SEQ ID NO. :406.

9. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region 322 WO 2021/226560 PCT/US2021/031442 (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRHI, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in SEQ ID NOs.:525, 526, 527, 529, 530, and 531, respectively,and wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

10. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRHI, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in SEQ ID NOs.:585, 586 or 625, 587 or 627, 589, 590, and 591, respectively,and wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

11. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising complementarity determining region (CDR)H1, CDRH2, and CDRH3, and a light chain variable domain (VL) comprising CDRL1, CDRL2, and CDRL3, wherein CDRHI, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 comprise the amino acid sequences set forth in SEQ ID NOs.:229, 230, 231, 233, 234, and 235, respectively,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

12. The antibody or antigen-binding fragment of any one of claims 1-11, wherein: 323 WO 2021/226560 PCT/US2021/031442 (i) the VH comprises or consists of an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence according to any one of SEQ IDNOs.: 399, 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 254, 264, 274, 284, 298, 312, 322, 332,350, 351, 353, 359, 361, 363, 365, 367, 368, 369, 379, 389, 409, 419, 429, 434, 444,454, 464, 474, 484, 494, 504, 514, 524, 534, 544, 554, 564, 574, 584, 594, 604, 614,624, 626, 628, 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658,660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 692, 740, 741, 742,743, 748, 749, 750, 752, 754, 756, 758, 759, 761, 762, and 764, wherein the variation is optionally limited to one or more framework regions and/or the variation comprises one or more substitution to a germline-encoded amino acid; and/or(ii) the VL comprises or consists of an amino acid sequence having at least 85% (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence according to any one of SEQ IDNOs.: 738, 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133, 143, 154, 157, 168,194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278, 288, 294, 296, 302,308, 310, 316, 326, 336, 355, 357, 373, 383, 393, 403, 413, 423, 438, 448, 458, 468,478, 488, 498, 508, 518, 528, 538, 548, 558, 568, 578, 588, 598, 608, 618, 686, 696,744, and 746, wherein the variation is optionally limited to one or more framework regions and/or the variation comprises one or more substitution to a germline-encoded amino acid.

13. The antibody or antigen-binding fragment of any one of claims 1- 12, wherein the VH comprises or consists of an amino acid sequence having at least 85% identity to the amino acid sequence set forth in SEQ ID NO.:399 and the VL comprises or consists of an amino acid sequence having at least 85% identity to the amino acid sequence set forth in SEQ ID NO. :403 or SEQ ID NO.:738. 324 WO 2021/226560 PCT/US2021/031442

14. The antibody or antigen-binding fragment of any one of claims 1-13, wherein the VH comprises or consists of an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO.:399 and the VL comprises or consists of an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

15. The antibody or antigen-binding fragment of any one of claims 1-14, wherein the VH comprises or consists of an amino acid sequence having at least 95% identity to the amino acid sequence set forth in SEQ ID NO.:399 and the VL comprises or consists of an amino acid sequence having at least 95% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

16. The antibody or antigen-binding fragment of any one of claims 1-15, wherein the VH comprises or consists of an amino acid sequence having at least 97% identity to the amino acid sequence set forth in SEQ ID NO.:399 and the VL comprises or consists of an amino acid sequence having at least 97% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

17. The antibody or antigen-binding fragment of any one of claims 1-16, wherein the VH comprises or consists of an amino acid sequence having at least 99% identity to the amino acid sequence set forth in SEQ ID NO.:399 and the VL comprises or consists of an amino acid sequence having at least 99% identity to the amino acid sequence set forth in SEQ ID NO.:403 or SEQ ID NO.:738.

18. The antibody or antigen-binding fragment of any one of claims 1-12, wherein the VH and the VL have at least 85% identity (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) to the amino acid sequences set forth in:(i) SEQ ID NOs.:524 and 528, respectively;(ii) SEQ ID NOs.:584 or 624 or 626 or 628 and 588, respectively; 325 WO 2021/226560 PCT/US2021/031442 (ill) SEQ ID NOs.:228 or 740 or 741 or 742 or 743 and 232, respectively; or(iv) SEQ ID NOs.:228 or 740 or 741 or 742 or 743 and 238, respectively.

19. The antibody or antigen-binding fragment of any one of claims 1- 18, wherein the VH comprises or consists of any VH amino acid sequence set forth in Table 2, and wherein the VL comprises or consists of any VL amino acid sequence set forth in Table 2, wherein, optionally, the VH and the VL comprise or consist of the amino acid sequences according to SEQ ID NOs.:(i) 399 and 403 or 738, respectively;(ii) 32 and 36, respectively;(iii) 42 and 46, respectively;(iv) 52 and 56, respectively;(v) 62 and 66, respectively;(vi) 72 and 66, respectively;(vii) 74 and 78, respectively;(viii) 84 and 88, respectively;(ix) 84 and 88, respectively;(x) 96 and 100, respectively;(xi) 106 and 110, respectively;(xii) 119 and 123, respectively; or(xiii) 129 and 133, respectively;(xiv) 22 or 150 and 26, 154, or 157, respectively;(xv) 42 or 163 and 46 or 168, respectively;(xvi) any one of 129, 173, 175, or 178 and 133, respectively;(xvii) any one of 52, 186, 189, or 191 and any one of 56, 194, or 196, respectively;(xviii) 198 and 202, respectively;(xix) 208 and 212, respectively; 326 WO 2021/226560 PCT/US2021/031442 (xx) 218 and 222, respectively;(xxi) 228 and 232 or 238, respectively;(xxii) 240 and any one of 244, 250, or 252, respectively;(xxiii) 254 and 258, respectively;(xxiv) 264 and 268, respectively;(xxv) 274 and 278, respectively; or(xxvi) 284 and any one of 288, 294, or 296, respectively;(xxvii) 298 and any one of 302, 308, or 310, respectively;(xxviii) 312 and 316, respectively;(xxix) 322 and 326, respectively;(xxx) 332 and 336, respectively;(xxxi) any one of 228, 350, 351, or 353 and 232, 238, 355, or 357, respectively;(xxxii) any one of 312, 359, 361, 363, 365, 367, or 368 and 316, respectively;(xxxiii) 369 and 373, respectively;(xxxiv) 379 and 383, respectively;(xxxv) 389 and 393, respectively;(xxxvi) 22 and 26, respectively;(xxxvii) 409 and 413, respectively;(xxxviii) 419 and 423, respectively;(xxxix) 434 and 438, respectively;(xxxx) 444 and 448, respectively;(xxxxi) 454 and 458, respectively;(xxxxii) 464 and 468, respectively;(xxxxiii) 474 and 478, respectively;(xxxxiv) 484 and 488, respectively;(xxxxv) 494 and 498, respectively;(xxxxvi) 504 and 508, respectively;(xxxxvii) 514 and 518, respectively;(xxxxviii) 524 and 528, respectively; 327 WO 2021/226560 PCT/US2021/031442 (xxxxix) 534 and 538, respectively;(xxxxx) 544 and 548, respectively;(xxxxxi) 554 and 558, respectively;(xxxxxii) 564 and 568, respectively;(xxxxxiii) 574 and 578, respectively;(xxxxxiv) 584 and 588, respectively;(xxxxxv) 594 and 598, respectively;(xxxxxvi) 604 and 608, respectively;(xxxxxvii) 614 and 618, respectively;(xxxxxviii) 624, 626, or 628 and 588, respectively;(xxxxxix) 630, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, or 684, and 686, respectively;(xxxxxx) 692 and 696, respectively;(xxxxxxi) any one of 740-743 and 238, respectively;(xxxxxxii) any one of 399, 748, 749, 750, 752, 754, 756, 758, 759, and 761 and any one of 403, 744, and 746, respectively; or(xxxxxxiii) 762 or 764 and 588, respectively.

20. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO:399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:738, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

21. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the 328 WO 2021/226560 PCT/US2021/031442 VH comprises or consists of the amino acid sequence set forth in SEQ ID NO:399 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:403, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

22. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:403, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

23. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:738, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

24. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:744, 329 WO 2021/226560 PCT/US2021/031442 wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

25. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs:399, 748, 749, 750, 752, 754, 756, 758, 759, and 761, the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:746,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

26. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in SEQ ID NO:524 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:528, wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

27. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs.:584, 624, 626, and 628 and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:588,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion. 330 WO 2021/226560 PCT/US2021/031442

28. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs.:228, 740, 741, 742, and 743, and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:232,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

29. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence set forth in any one of SEQ ID NOs.:228, 740, 741, 742, and 743, and the VL comprises or consists of the amino acid sequence set forth in SEQ ID NO:238,wherein the antibody or antigen-binding fragment is capable of binding to a surface glycoprotein of SARS-CoV-2 expressed on a cell surface of a host cell and/or on a virion.

30. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH comprises or consists of the amino acid sequence as set forth in SEQ ID NO: and the VL comprises or consists of the amino acid sequence as set forth in SEQ ID NO: 36.

31. An antibody, or an antigen-binding fragment thereof, comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 33-35, respectively, and the CDRL1, CDRL2, and 331 WO 2021/226560 PCT/US2021/031442 CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 37- 39, respectively.

32. The antibody or antigen-binding fragment of any one of claims 3-31, which is capable of neutralizing a SARS-CoV-2 infection in an in vitro model of infection and/or in an in vivo animal model of infection and/or in a human.

33. The antibody or antigen-binding fragment of any one of claims 1-32, which:(i) recognizes an epitope in the ACE2 receptor binding motif (RBM, SEQ ID NO. :5) of SARS-CoV-2;(ii) is capable of blocking an interaction between SARS-CoV-2 (e.g., SARS- C0V-2 RBM) and human ACE2;(iii) is capable of binding to SARS-C0V-2S protein;(iv) recognizes an epitope that is conserved in the ACE2 RBM of SARS- C0V-2 and in an ACE2 RBM of SARS-CoV-1;(v) is cross-reactive against SARS-CoV-2 and SARS-CoV-1 coronavirus;(vii) recognizes an epitope in the SARS-CoV-2 surface glycoprotein that is not in the ACE2 RBM;(viii) is capable of binding to a SARS-CoV-2 S protein trimer in a prefusion conformation;or(ix) any combination of (i)-(viii).

34. The antibody or antigen-binding fragment of any one of claims 1-33, which is a IgG, IgA, IgM, IgE, or IgD isotype.

35. The antibody or antigen-binding fragment of any one of claims 1-34, which is an IgG isotype selected from IgGl, IgG2, IgG3, and IgG4. 332 WO 2021/226560 PCT/US2021/031442

36. The antibody or antigen-binding fragment of any one of claims 1-35, which is human, humanized, or chimeric.

37. The antibody or antigen-binding fragment of any one of claims 1-36, wherein the antibody, or the antigen-binding fragment, comprises a human antibody, a monoclonal antibody, a purified antibody, a single chain antibody, a Fab, a Fab’, a F(ab’)2, a Fv, a scFv, or a scFab.

38. The antibody or antigen-binding fragment of claim 37, wherein the scFv comprises more than one VH domain and more than one VL domain.

39. The antibody or antigen-binding fragment of any one of claims 1-38, wherein the antibody or antigen-binding fragment is a multi-specific antibody or antigen binding fragment.

40. The antibody or antigen-binding fragment of claim 39, wherein the antibody or antigen binding fragment is a bispecific antibody or antigen-binding fragment.

41. The antibody or antigen-binding fragment of claim 39 or 40, comprising:(i) a first VH and a first VL; and(ii) a second VH and a second VL,wherein the first VH and the second VH are different and each independently comprise an amino acid sequence having at least 85% (e.g., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 22, 32, 42, 52, 62, 72, 74, 84, 96, 106, 119, 129, 139, 150, 163, 173, 175, 178, 186, 189, 191, 198, 208, 218, 228, 240, 254, 264, 274, 284, 298, 312, 322, 332, 350, 351, 353, 359, 361, 363, 365, 367,368, 369, 379, 389, 399, 409, 419, 429, 434, 444, 454, 464, 474, 484, 494, 504, 514,524, 534, 544, 554, 564, 574, 584, 594, 604, 614, 624, 626, 628, 630, 634, 636, 638, 333 WO 2021/226560 PCT/US2021/031442 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666, 668, 670, 672, 674, 676, 678, 680, 682, 684, 692, 740, 741, 742, 743, 748, 749, 750, 752, 754, 756, 758, 759, 761, 762, and 764, andwherein the first VL and the second VL are different and each independently comprise an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 26, 36, 46, 56, 66, 78, 88, 94, 100, 110, 123, 133, 143, 154, 157, 168, 194, 196, 202, 212, 222, 232, 238, 244, 250, 252, 258, 268, 278, 288, 294, 296, 302, 308, 310, 316, 326, 336, 355, 357, 373, 383,393, 403, 413, 423, 438, 448, 458, 468, 478, 488, 498, 508, 518, 528, 538, 548, 558,568, 578, 588, 598, 608, 618, 686, 696 738, 744, and 746;and wherein the first VH and the first VL together form a first antigen-bindingsite, and wherein the second VH and the second VL together form a second antigen- binding site.

42. The antibody or antigen-binding fragment of claim 40 or 41, comprising:(i) a first VH and a first VL; and(ii) a second VH and a second VL,wherein the first VH comprises an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 139 and 342 and the first VL comprises an amino acid sequence having at least 85% (i.e., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 143 and 346 and wherein the second VH comprises an amino acid sequence having at least 85% (e.g, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) identity to the amino acid sequence set forth in any one of SEQ ID NOs.: 399, 748, 749, 750, 752, 754, 756, 758, 759, and 761 and the second VL comprises an amino acid sequence having at least 85% (i.e., 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) 334 WO 2021/226560 PCT/US2021/031442 identity to the amino acid sequence set forth in any one of SEQ ID NOs.:403, 744, and 746.

43. The antibody or antigen-binding fragment of claim 39 or 40, comprising a first antigen-binding portion having a first specificity and a second antigen-binding portion having a second specificity, wherein the first antigen-binding portion comprises a VH that comprises or consists of the amino acid sequence set forth in SEQ ID NO:3and a VL that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :738 or SEQ ID NO. :403.

44. The antibody or antigen-binding fragment of claim 43, wherein the second antigen-binding portion comprises a VH that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :139 and a VL that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :143.

45. The antibody or antigen-binding fragment of claim 43, wherein the second antigen-binding portion comprises a VH that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :342 and a VL that comprises or consists of the amino acid sequence set forth in SEQ ID NO. :346.

46. The antibody or antigen-binding fragment of any one of claims 1-45, wherein the antibody or antigen-binding fragment further comprises a Fc polypeptide or a fragment thereof.

47. The antibody or antigen-binding fragment of claim 46, wherein the Fc polypeptide or fragment thereof comprises:(i) a mutation that enhances binding to a FcRn as compared to a reference Fc polypeptide that does not comprise the mutation; and/or(ii) a mutation that enhances binding to a FcyR as compared to a reference Fc polypeptide that does not comprise the mutation. 335 WO 2021/226560 PCT/US2021/031442

48. The antibody or antigen-binding fragment of claim 47, wherein the mutation that enhances binding to a FcRn comprises: M428L; N434S; N434H; N434A; N434S; M252Y; S254T; T256E; T250Q; P257I; Q31II; D376V; T307A; E380A; or any combination thereof.

49. The antibody or antigen-binding fragment of claim 47 or 48, wherein the mutation that enhances binding to FcRn comprises:(i) M428L/N434S;(ii) M252Y/S254T/T256E;(iii) T250Q/M428L;(iv) P257VQ311I;(v) P2571/N434H;(vi) D376V/N434H;(vii) T307A/E380A/N434A; or(viii) any combination of (i)-(vii).

50. The antibody or antigen-binding fragment of any one of claims 47-49, wherein the mutation that enhances binding to FcRn comprises M428L/N434S.

51. The antibody or antigen-binding fragment of any one of claims 47-50, wherein the mutation that enhances binding to a FcyR comprises S239D; I332E; A330L; G236A; or any combination thereof.

52. The antibody or antigen-binding fragment of any one of claims 47-51, wherein the mutation that enhances binding to a FcyR comprises:(i) S239D/I332E;(ii) S239D/A330L/I332E;(iii) G236A/S239D/1332E; or(iv) G236A/A330L/I332E. 336 WO 2021/226560 PCT/US2021/031442

53. The antibody or antigen-binding fragment of any one of claims 47-52, wherein the Fc polypeptide comprises a L234A mutation and a L235A mutation.

54. The antibody or antigen-binding fragment of any one of claims 1-53, which comprises a mutation that alters glycosylation, wherein the mutation that alters glycosylation comprises N297A, N297Q, or N297G, and/or which is aglycosylated and/or afucosylated.

55. The antibody or antigen-binding fragment of any one of claims 1-54, which is capable of binding to a SARS-C0V-2 surface glycoprotein with an EC50 of less than 500 ng/ml, less than 250 ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 16 ng/ml, less than 15 ng/ml, less than 14 ng/ml, less than 13 ng/ml, less than 12 ng/ml, less than ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, or less than 2 mg/ml, as measured by ELISA (optionally, indirect ELISA and/or sandwich ELISA) and/or by flow cytometry, wherein the SARS C0V-2 surface glycoprotein is expressed at a cell surface of a host cell.

56. The antibody or antigen-binding fragment of any one of claims 1-55, which is capable of binding to a SARS-CoV-2 surface glycoprotein RED with an ECof less than 500 ng/ml, less than 250 ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 50 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 16 ng/ml, less than 15 ng/ml, less than 14 ng/ml, less than 13 ng/ml, less than 12 ng/ml, less than ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, or less than 2 mg/ml, as measured by ELISA (optionally, indirect ELISA and/or sandwich ELISA) and/or by flow cytometry, 337 WO 2021/226560 PCT/US2021/031442 wherein the SARS C0V-2 surface glycoprotein is expressed at a cell surface of a host cell.

57. The antibody or antigen-binding fragment of any one of claims 1-56, which is capable of binding to a SARS-CoV-2 RBD with a KD of less than 5 x 108־ M, less than 4 x 108־ M, less than 3 x 108־ M, less than 2 x 108־ M, less than 1 x 108־ M, less than 5 x 109־ M, less than 1 x 109־ M, less than 5 x 1010־ M, less than 1 x 1010־ M, less than 5 x 1011־ M, less than 1 x 1011־ M, less than 5 x 1012־ M, or less than 1 x 1012־M, as determined using biolayer interferometry (BLI), optionally using an Octet instrument with antibody or antigen-binding fragment loaded on Protein A pins, optionally at 2.ug/ml, and SARS-CoV-2 RBD loaded for 5 minutes at 6 ug/ml, 1.5 ug/ml, or 0.ug/ml, further optionally with dissociation measured for 7 minutes.

58. The antibody or antigen-binding fragment of any one of claims 1-57, which is capable of binding to a SARS-CoV-2 RBD with a KD of less than 6 x 108־ M, less than 5 x 108־ M, less than 4 x 108־ M, less than 3 x 108־ M, less than 2 x 108־ M, less than 1 x 108־ M, less than 5 x 109־ M, less than 4 x 109־ M, less than 3 x 109־ M, less than 2 x 109־ M, less than 1 x 109־ M, or less than 8 x 1010־ M, as determined using surface plasmon resonance (SPR), optionally using a Biacore T200 instrument using a single-cycle kinetics approach.

59. The antibody or antigen-binding fragment of any one of claims 1-58, which is capable of binding to a SARS-CoV-2 RBD and inhibiting an interaction between (i) the RBD and (ii) a human ACE2 and/or a human SIGLEC-1.

60. The antibody or antigen-binding fragment of any one of claims 1-59, which is capable of neutralizing:(i) infection by a SARS-CoV-2 pseudovirus, optionally:(i)(a) with a neutralization IC50 of less than 100 ng/ml, less than ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than 338 WO 2021/226560 PCT/US2021/031442 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than ng/ml, less than 15 ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, less than 3 ng/ml, less than 2 ng/ml, or less than 1 ng/ml, preferably less than ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less than 6 ng/ml, less than 5 ng/ml, less than 4 ng/ml, less than 3 ng/ml, less than 2 ng/ml, or less than 1 ng/ml, and/or(i)(b) with a neutralization IC80 of less than 100 ng/ml, less than ng/ml, less than 80 ng/ml, less than 70 ng/ml, less than 60 ng/ml, less than ng/ml, less than 40 ng/ml, less than 30 ng/ml, or less than 25 ng/ml, preferably less than 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, or less than 25 ng/ml, and/or(i)(c) with a neutralization EC90 of less than 300 ng/ml, less than 2ng/ml, less than 100 ng/ml, less than 90 ng/ml, less than 80 ng/ml, less than ng/ml, less than 60 ng/ml 50 ng/ml, less than 40 ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 15 ng/ml, or less than 10 ng/ml, wherein, further optionally, the SARS-CoV-2 pseudovirus comprises a VSV pseudovirus and/or a MLV pseudovirus, and/or(i)(d) the SARS-CoV-2 pseudovirus comprises a VSV pseudovirus and/or a MLV pseudovirus; and/or(ii) infection by live SARS-CoV-2, optionally(ii)(a) with a EC50 of less than 60 ng/ml, less than 50 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, preferably less than 15 ng/ml, less than 12 ng/ml, less than ng/ml, less than 10 ng/ml, less than 9 ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, and/or(ii)(b) with a EC90 of less than 50 ng/ml, less than 40 ng/ml, less than ng/ml, less than 30 ng/ml, less than 25 ng/ml, less than 20 ng/ml, less than 339 WO 2021/226560 PCT/US2021/031442 ng/ml, less than 12 ng/ml, less than 11 ng/ml, less than 10 ng/ml, less than ng/ml, less than 8 ng/ml, less than 7 ng/ml, less 6 ng/ml, less than 5/ng ml, or less than 4 ng/ml, preferably less than 30 ng/ml, less than 25 ng/ml, less than ng/ml, less than 15 ng/ml, or less than 12 ng/ml, and/or(ii)(c) over a 6-hour period, with a multiplicity of infection of 0.1; and/or(iii) infection by live SARS-CoV-2 in a host cell (e.g. a HEK293T cell) that expresses, optionally is engineered to overexpress, DC-SIGN, L-SIGN, SIGLEC, or ACE2; and/or(iv) infection by live SARS-CoV-2 in a host cell (e.g. a HEK293T cell) that expresses, optionally is engineered to overexpress, SIGLEC-1 or ACE2, wherein neutralizing infection comprises fully neutralizing infection.

61. The antibody or antigen-binding fragment of any one of claims 1-60, which is capable of neutralizing infection by a SARS-CoV-2 variant that comprises any one of the following mutations in the surface glycoprotein as compared to a SAKS- C0V-2 surface glycoprotein comprising SEQ ID NO.:3: N501Y; S477N; N439K; L452R; E484K; K417N; T478K; S494P; A520S; N501T; A522S; Y453F; P384L.

62. The antibody or antigen-binding fragment of claim 61, which is capable of neutralizing infection by the SARS-CoV-2 variant with a potency that is less than 3- fold lower than the potency with which the antibody or antigen-binding fragment neutralizes infection by a SARS-CoV-2 comprising the surface glycoprotein amino acid sequence set forth in SEQ ID NO. :3.

63. The antibody or antigen-binding fragment of any one of claims 1-62, which is capable of activating a FcyRIIa, a FcyRIIIa, or both, wherein, optionally:(!)the FcyRIIa comprises a H131 allele; and/or(ii) the FcyRIIIa comprises a VI58 allele; and/or 340 WO 2021/226560 PCT/US2021/031442 (iii) activation is determined using a SARS-CoV-2 S-expressing target cell, such as a CHO cell, and a reporter cell expressing a NFAT-driven reporter, such as luciferase.

64. The antibody or antigen-binding fragment of any one of claims 1-63, comprising:(a) the CH1-CH3 amino acid sequence set forth in SEQ ID NO. :6 and the CL amino acid sequence set forth in SEQ ID NO. :8;(b) The CH1-CH3 amino acid sequence set forth in SEQ ID NO.:6 and the CL amino acid sequence set forth in SEQ ID NO.:9;(c) the CH1-CH3 amino acid sequence set forth in SEQ ID NO. :7 and the CL amino acid sequence set forth in SEQ ID NO. :8; or(d) The CH1-CH3 amino acid sequence set forth in SEQ ID NO.:7 and the CL amino acid sequence set forth in SEQ ID NO. :9.

65. An isolated antibody comprising:(i) the heavy chain amino acid sequence set forth in SEQ ID NO. :767; and (ii) the light chain amino acid sequence set forth in SEQ ID NO.:768.

66. The antibody or antigen-binding fragment of any one of claims 1-65, which has an in vivo half-life in a non-human primate of between 20 and 30 days, or between 22 and 28 days, or between 23 and 27 days, or between 24 and 26 days, or of about 25 days.

67. The antibody or antigen-binding fragment of any one of claims 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS- C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 20 to about 30 ng/ml. 341 WO 2021/226560 PCT/US2021/031442

68. The antibody or antigen-binding fragment of any one of claims 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS- C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 10 to about 20 ng/ml.

69. The antibody or antigen-binding fragment of any one of claims 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS- C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 5 to about 10 ng/ml.

70. The antibody or antigen-binding fragment of any one of claims 1-66, wherein the antibody or antigen-binding fragment is capable of neutralizing a SARS- C0V-2 infection and/or of neutralizing an infection of a target cell with an IC50 of about 1 to about 5 ng/ml.

71. The antibody or antigen-binding fragment of any one of claims 1-70, wherein the antibody or antigen-binding fragment is capable of neutralizing infection by SARS-C0V-2 and does not compete with a human ACE2 for binding to the SARS- C0V-2S protein,wherein, optionally, the neutralizing comprises neutralizing infection in an in vitro model of infection.

72. An antibody, or an antigen-binding fragment thereof, that competes for binding to a SARS-C0V-2 surface glycoprotein with the antibody or antigen-binding fragment of any one of claims 1-71.

73. An isolated polynucleotide encoding the antibody or antigen-binding fragment of any one of claims 1-72, or encoding a VH, a heavy chain, a VL, and/or a light chain of the antibody or the antigen-binding fragment. 342 WO 2021/226560 PCT/US2021/031442

74. The polynucleotide of claim 73, wherein the polynucleotide comprises deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), wherein the RNA optionally comprises messenger RNA (mRNA).

75. The polynucleotide of claim 73 or 74, which is codon-optimized for expression in a host cell.

76. The polynucleotide of any one of claims 73-75, comprising a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or comprises or consists of, the polynucleotide sequence according to any one or more of SEQ ID NOs.: 30, 31, 40, 41, 50, 51, 60, 61, 70, 71, 73, 82, 83, 92, 93, 95, 104, 105, 114, 115, 116, 117, 118, 127, 128, 137, 138, 206, 207, 216, 217, 226, 227, 236, 237, 239, 248, 249, 251, 253, 262, 263, 272, 273, 282, 283,292, 293, 295, 297, 306, 307, 309, 311, 320, 321, 330, 331, 340, 341, 377, 378, 387,388, 397, 398, 407, 408, 417, 418, 427, 428, 433, 442, 443, 452, 453, 462, 463, 472,473, 482, 483, 492, 493, 502, 503, 512, 513, 552, 523, 532, 533, 542, 543, 552, 553,562, 563, 572, 573, 582, 583, 592, 593, 602, 603, 612, 613, 622, 623, 690, 691, 700-737and 739.

77. The polynucleotide of any one of claims 73-76, comprising:(i) a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or that comprises or consists of, the nucleotide sequence set forth in SEQ ID NO. :407; and(ii) a polynucleotide having at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to, or that comprises or consists of, the nucleotide sequence set forth in SEQ ID NO. :408, 737, or 739. 343 WO 2021/226560 PCT/US2021/031442

78. A recombinant vector comprising the polynucleotide of any one of claims 73-77.

79. A host cell comprising the polynucleotide of any one of claims 77 and/or the vector of claim 78, wherein the polynucleotide is heterologous to the host cell.

80. A human B cell comprising the polynucleotide of any one of claims 73- 77, wherein polynucleotide is heterologous to the human B cell and/or wherein the human B cell is immortalized.

81. A composition comprising:(i) the antibody or antigen-binding fragment of any one of claims 1-72;(ii) the polynucleotide of any one of claims 73-77;(iii) the recombinant vector of claim 78;(iv) the host cell of claim 79; and/or(v) the human B cell of claim 80, and a pharmaceutically acceptable excipient, carrier, or diluent.

82. The composition of claim 81, comprising two or more antibodies or antigen-binding fragments of any of claims 1-72.

83. The composition of claim 82, comprising:(i) a first antibody or antigen-binding fragment, comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 32 and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 36; and(ii) a second antibody or antigen-binding fragment comprising, a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 1and a VL comprising of consisting of the amino acid sequence as set forth in SEQ ID NO: 143. 344 WO 2021/226560 PCT/US2021/031442

84. The composition of claim 82, comprising:(i) a first antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 33-35, respectively, and the CDRL1, CDRL2, and CDRLcomprise or consist of the amino acid sequences set forth in SEQ ID NOs: 37-39, respectively; and(ii) a second antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 140-142, respectively, and the CDRL1, CDRL2, and CDRLcomprise or consist of the amino acid sequences set forth in SEQ ID NOs: 144-146, respectively.

85. The composition of claim 82, comprising:(i) a first antibody or antigen-binding fragment, comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 139 or 342 and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 143 or 346; and(ii) a second antibody or antigen-binding fragment comprising a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761, and a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 403, 744, or 746.

86. The composition of claim 82, comprising:(i) a first antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein 345 WO 2021/226560 PCT/US2021/031442 the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 140-142, respectively, or 343-345, respectively, and the CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 144-146, respectively; and(ii) a second antibody or antigen-binding fragment comprising a heavy chain variable domain (VH) comprising a CDRH1, a CDRH2, and a CDRH3, and a light chain variable domain (VL) comprising a CDRL1, a CDRL2, and a CDRL3, wherein the CDRH1, CDRH2, and CDRH3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively, and the CDRL1, CDRL2, and CDRL3 comprise or consist of the amino acid sequences set forth in SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively.

87. A composition comprising:(i) a first antibody or antigen-binding fragment, comprising(i)(a) a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 32, and(i)(b) a VL comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 36; and(ii) a second antibody or antigen-binding fragment comprising(ii)(a) a VH comprising or consisting of the amino acid sequence as set forth in SEQ ID NO: 139 and(ii)(b) a VL comprising of consisting of the amino acid sequence as set forth in SEQ ID NO: 143.

88. A composition comprising:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-C0V-2 surface glycoprotein and comprises(i)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs. :400, 402, and 766, respectively, and 346 WO 2021/226560 PCT/US2021/031442 (i)(b) a VL comprising the CDRL1, CDRL2, and CDRL3 amino acid sequences set forth in SEQ ID NOs.:404, 405, and 406, respectively; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:140, 141 or 343, and 142, respectively, and(ii)(b) a VL comprising CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.:144, 145, and 146, respectively.

89. A composition comprising:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the amino acid sequence set forth in SEQ ID NO.:399 and(i)(b) a VL comprising the amino acid sequence set forth in SEQ ID NOs.:403 or SEQ ID NO.:738; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the amino acid sequence set forth in SEQ ID NOs.:139 or 342, and(ii)(b) a VL comprising the amino acid sequence set forth in SEQ ID NO.:143.

90. The composition of any one of claims 82-89, wherein the first antibody or antigen-binding fragment and the second antibody or antigen-binding fragment each comprise an IgGl Fc polypeptide comprising a M428L mutation and a N434S mutation. 347 WO 2021/226560 PCT/US2021/031442

91. The composition of any one of claims 82-90, wherein the first antibody or antigen-binding fragment and the second antibody or antigen-binding fragment each comprise an IgGl Fc polypeptide comprising a G236A mutation, a A330L mutation, and a I332E mutation.

92. A composition comprising the polynucleotide of any one of claims 73-encapsulated in a carrier molecule, wherein the carrier molecule optionally comprises a lipid, a lipid-derived delivery vehicle, such as a liposome, a solid lipid nanoparticle, an oily suspension, a submicron lipid emulsion, a lipid microbubble, an inverse lipid micelle, a cochlear liposome, a lipid microtubule, a lipid microcylinder, lipid nanoparticle (LNP), or a nanoscale platform.

93. A composition comprising:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS- C0V-2 surface glycoprotein and a first cell surface receptor selected from ACE2, DC- SIGN, L-SIGN, and SIGLEC-1; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS-CoV-2 surface glycoprotein and a second cell surface receptor selected from ACE2, DC-SIGN, L-SIGN, and SIGLEC-1,wherein the first cell surface receptor and the second cell surface receptor are different.

94. A method of treating a coronavirus infection, e.g. a SARS-CoV-infection, in a subject, the method comprising administering to the subject an effective amount of:(i) the antibody or antigen-binding fragment of any one of claims 1-72;(ii) the polynucleotide of any one of claims 73-77;(iii) the recombinant vector of claim 78; 348 WO 2021/226560 PCT/US2021/031442 (iv) the host cell of claim 79;(v) the human B cell of claim 80; and/or(vi) the composition of any one of claims 81-93.

95. A method of treating a coronavirus infection, e.g. a SARS-CoV-infection, in a subject, the method comprising administering to the subject:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:400, 402, and 766, respectively, and(i)(b) a VL comprising the CDRL1, CDRL2, and CDRL3 amino acid sequences set forth in SEQ ID NOs.:404, 405, and 406, respectively; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the CDRH1, CDRH2, and CDRH3 amino acid sequences set forth in SEQ ID NOs.:140, 141 or 343, and 142, respectively, and(ii)(b) a VL comprising CDRL1, CDRL2, and CDRL3 amino acid sequences as set forth in SEQ ID NOs.:144, 145, and 146, respectively.

96. A method of treating a coronavirus infection, e.g. a SARS-CoV-infection, in a subject, the method comprising administering to the subject:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(i)(a) a VH comprising the amino acid sequence set forth in SEQ ID NO.:399 and 349 WO 2021/226560 PCT/US2021/031442 (i)(b) a VL comprising the amino acid sequence set forth in SEQ ID NOs.:403 or SEQ ID NO.:738; and(ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and comprises(ii)(a) a VH comprising the amino acid sequence set forth in SEQ ID NOs.: 139 or 342, and(ii)(b) a VL comprising the amino acid sequence set forth in SEQ ID NO.:143.

97. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) VH and VL amino acid sequences according to SEQ ID NOs. :and 36 respectively; or(i)(b) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOS.:33-35 and 37-39, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) a VH amino acid sequence according to SEQ ID NO.: 139, and aVL amino acid sequence according to SEQ ID NO: 143; or(ii)(b) CDRH1, CDRH2, and CDRH3 amino acids according to SEQ ID NOs: 140-142, respectively, and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 144-146.

98. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) a VH amino acid sequence according to SEQ ID NO.: 139, and aVL amino acid sequence according to SEQ ID NO: 143; or 350 WO 2021/226560 PCT/US2021/031442 (i)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 140-142, respectively; and CDRL1, CDRL2, and CDRLamino acid sequences according to SEQ ID NOs: 144-146, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) VH and VL amino acid sequences according to SEQ ID NOs.:and 36 respectively; or(ii)(b) CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOS.:33-35 and 37-39, respectively.

99. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising:(i)(a) a VH amino acid sequence according to SEQ ID NO.: 139 or 342 and a VL amino acid sequence according to SEQ ID NO: 143 or 346; or(i)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 140-142, respectively, or 343-345, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 144-146, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) a VH amino acid sequence according to SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761 and a VL amino acid sequence according to SEQ ID NO: 403, 744, or 746; or(ii)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively.

100. A method of preventing or treating or neutralizing a coronavirus infection in a subject, the method comprising administering to a subject who has received a first antibody or antigen binding fragment comprising: 351 WO 2021/226560 PCT/US2021/031442 (i)(a) a VH amino acid sequence according to SEQ ID NO: 399, 748, 749, 750, 752, 754, 756, 758, 759, or 761 and a VL amino acid sequence according to SEQ ID NO: 403, 744, or 746; or(i)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ IDNOs: 400, 401, and any one of 751, 753, 755, 757, 760, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 404, 405, and any one of 406, 745, and 747, respectively;a second antibody or antigen binding fragment comprising:(ii)(a) a VH amino acid sequence according to SEQ ID NO.: 139 or 342 and a VL amino acid sequence according to SEQ ID NO: 143 or 346; or(ii)(b) CDRH1, CDRH2, and CDRH3 amino acid sequences according to SEQ ID NOs: 140-142, respectively, or 343-345, respectively and CDRL1, CDRL2, and CDRL3 amino acid sequences according to SEQ ID NOs: 144-146, respectively.

101. The method of any one of claims 95-100, wherein the first antibody or antigen-binding fragment and the second antibody or antigen-binding fragment each comprise an IgGl Fc polypeptide comprising a M428L mutation and a N434S mutation.

102. The method of any one of claims 95-101, wherein the first antibody or antigen-binding fragment and the second antibody or antigen-binding fragment each comprise an IgGl Fc polypeptide comprising a G236A mutation, a A330L mutation, and a I332E mutation.

103. A method of treating a SARS-CoV-2 infection in a subject, the method comprising administering to the subject:(i) a first antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS- C0V-2 surface glycoprotein and a first cell surface receptor selected from ACE2, DC- SIGN, L-SIGN, and SIGLEC-1; and 352 WO 2021/226560 PCT/US2021/031442 (ii) a second antibody or antigen-binding fragment that is capable of binding to a SARS-CoV-2 surface glycoprotein and inhibiting an interaction between the SARS-CoV-2 surface glycoprotein and a second cell surface receptor selected from ACE2, DC-SIGN, L-SIGN, and SIGLEC-1,wherein the first cell surface receptor and the second cell surface receptor are different.

104. The antibody or antigen-binding fragment of any one of claims 1-72, the polynucleotide of any one of claims 73-77, the recombinant vector of claim 78, the host cell of claim 79, the human B cell of claim 80, and/or the composition of any one of claims 81-93 for use in a method of treating a SARS-CoV-2 infection in a subject.

105. The antibody or antigen-binding fragment of any one of claims 1-72, the polynucleotide of any one of claims 73-77, the recombinant vector of claim 78, the host cell of claim 79, the human B cell of claim 80, and/or the composition of any one of claims 81-93 for use in the preparation of a medicament for the treatment of a SARS- C0V-2 infection in a subject.

106. A method for in vitro diagnosis of a SARS-CoV-2 infection, the method comprising:(i) contacting a sample from a subject with an antibody or antigen-binding fragment of any one of claims 1-72; and(ii) detecting a complex comprising an antigen and the antibody, or comprising an antigen and the antigen-binding fragment.

107. The method of claim 106, wherein the sample comprises blood isolated from the subject. 353