IL292891B2 - מכשיר ושיטה של אלקטרופורציה - Google Patents

מכשיר ושיטה של אלקטרופורציה

Info

Publication number
IL292891B2
IL292891B2 IL292891A IL29289122A IL292891B2 IL 292891 B2 IL292891 B2 IL 292891B2 IL 292891 A IL292891 A IL 292891A IL 29289122 A IL29289122 A IL 29289122A IL 292891 B2 IL292891 B2 IL 292891B2
Authority
IL
Israel
Prior art keywords
electroporation apparatus
electroporation
Prior art date
Application number
IL292891A
Other languages
English (en)
Other versions
IL292891B1 (he
IL292891A (he
Inventor
Bryan Butman
Jonathan Carson
Thomas D Reed
Shuyuan Zhang
Travis Andrews
David Cerrone
Jeffrey Gentry
William Hock
Justin Inslee
Kraig Kruger
Mackenzie Miller
Scott Schmidt
Robert Schneider
Chad B Green
Vincent So
Original Assignee
Precigen Inc
Bryan Butman
Jonathan Carson
Thomas D Reed
Shuyuan Zhang
Travis Andrews
David Cerrone
Jeffrey Gentry
William Hock
Justin Inslee
Kraig Kruger
Mackenzie Miller
Scott Schmidt
Robert Schneider
Chad B Green
Vincent So
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Precigen Inc, Bryan Butman, Jonathan Carson, Thomas D Reed, Shuyuan Zhang, Travis Andrews, David Cerrone, Jeffrey Gentry, William Hock, Justin Inslee, Kraig Kruger, Mackenzie Miller, Scott Schmidt, Robert Schneider, Chad B Green, Vincent So filed Critical Precigen Inc
Publication of IL292891A publication Critical patent/IL292891A/he
Publication of IL292891B1 publication Critical patent/IL292891B1/he
Publication of IL292891B2 publication Critical patent/IL292891B2/he

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50855—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
IL292891A 2019-11-11 2022-05-09 מכשיר ושיטה של אלקטרופורציה IL292891B2 (he)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962933717P 2019-11-11 2019-11-11
US201962940032P 2019-11-25 2019-11-25
PCT/US2020/059984 WO2021096936A1 (en) 2019-11-11 2020-11-11 Electroporation apparatus and method

Publications (3)

Publication Number Publication Date
IL292891A IL292891A (he) 2022-07-01
IL292891B1 IL292891B1 (he) 2026-03-01
IL292891B2 true IL292891B2 (he) 2026-07-01

Family

ID=73646600

Family Applications (1)

Application Number Title Priority Date Filing Date
IL292891A IL292891B2 (he) 2019-11-11 2022-05-09 מכשיר ושיטה של אלקטרופורציה

Country Status (11)

Country Link
US (1) US12570945B2 (he)
EP (1) EP4058551A1 (he)
JP (1) JP7904785B2 (he)
KR (1) KR102994155B1 (he)
CN (1) CN114981400A (he)
AU (1) AU2020383419B2 (he)
BR (1) BR112022009068A2 (he)
IL (1) IL292891B2 (he)
MX (1) MX2022005663A (he)
PH (1) PH12022551127A1 (he)
WO (1) WO2021096936A1 (he)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117660176A (zh) * 2022-08-25 2024-03-08 苏州壹达生物科技有限公司 一种流式电穿孔组件
WO2024263564A2 (en) * 2023-06-21 2024-12-26 Lonza Cologne Gmbh Multi-chambered electroporation cartridge and methods of use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005113820A2 (en) * 2004-05-12 2005-12-01 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
EP1289660B1 (fr) * 2000-06-15 2006-02-08 Moussa Hoummady Systeme de distribution parallele et selective de micro-gouttelettes a haut rendement
EP2208779A1 (de) * 2009-01-20 2010-07-21 Lonza Cologne AG Behältnis mit mehreren Reaktionsräumen und Elektroden

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Publication number Priority date Publication date Assignee Title
US5720921A (en) * 1995-03-10 1998-02-24 Entremed, Inc. Flow electroporation chamber and method
CA2508358A1 (en) 2002-12-03 2004-06-17 Genetronics, Inc. Large-scale electroporation plates, systems, and methods of use
US6878538B1 (en) 2003-10-28 2005-04-12 Cyto Pulse Sciences, Inc. Apparatus for receiving and aligning a multiple electrode pair array and a multiple treatment cell for use in electroporation
DE502004009820D1 (de) 2004-03-15 2009-09-10 Lonza Cologne Ag Behältnis und Vorrichtung zur Erzeugung von elektrischen Feldern in einzelnen Reaktionsräumen
US20050282283A1 (en) 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
US7392823B2 (en) * 2004-12-01 2008-07-01 Parker-Hannifin Corporation Combination valve
US7799555B2 (en) 2006-02-10 2010-09-21 Bio-Rad Laboratories, Inc. Apparatus for high-throughput electroporation
US7943367B2 (en) 2006-09-20 2011-05-17 Bio-Rad Laboratories, Inc. High-throughput electroporation chamber with functional lid for risk reduction
US8043838B2 (en) 2008-02-20 2011-10-25 Bio-Rad Laboratories, Inc. Electroporation cuvette with spatially variable electric field
EP2603517B1 (en) * 2010-05-12 2023-07-19 Cellectis Dynamic mixing and electroporation chamber and system
PT3397337T (pt) * 2015-12-30 2020-11-24 Inovio Pharmaceuticals Inc Dispositivo de eletroporação com conjunto de agulhas destacável com sistema de bloqueio
US10233419B2 (en) * 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
US10435713B2 (en) * 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1289660B1 (fr) * 2000-06-15 2006-02-08 Moussa Hoummady Systeme de distribution parallele et selective de micro-gouttelettes a haut rendement
WO2005113820A2 (en) * 2004-05-12 2005-12-01 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
EP2208779A1 (de) * 2009-01-20 2010-07-21 Lonza Cologne AG Behältnis mit mehreren Reaktionsräumen und Elektroden

Also Published As

Publication number Publication date
PH12022551127A1 (en) 2023-08-23
KR102994155B1 (ko) 2026-07-22
AU2020383419A1 (en) 2022-05-26
KR20220098004A (ko) 2022-07-08
BR112022009068A2 (pt) 2022-07-26
MX2022005663A (es) 2022-08-25
JP7904785B2 (ja) 2026-08-13
US12570945B2 (en) 2026-03-10
AU2020383419B2 (en) 2026-04-23
CA3157944A1 (en) 2021-05-20
EP4058551A1 (en) 2022-09-21
US20210139837A1 (en) 2021-05-13
IL292891B1 (he) 2026-03-01
IL292891A (he) 2022-07-01
CN114981400A (zh) 2022-08-30
WO2021096936A1 (en) 2021-05-20
JP2022554401A (ja) 2022-12-28