IL167776A - Human foreskin cells suitable for culturing stem cells - Google Patents
Human foreskin cells suitable for culturing stem cellsInfo
- Publication number
- IL167776A IL167776A IL167776A IL16777605A IL167776A IL 167776 A IL167776 A IL 167776A IL 167776 A IL167776 A IL 167776A IL 16777605 A IL16777605 A IL 16777605A IL 167776 A IL167776 A IL 167776A
- Authority
- IL
- Israel
- Prior art keywords
- serum
- human
- cell culture
- cells
- foreskin
- Prior art date
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (16)
1. A method of maintaining embryonic stem cells in an undifferentiated state comprising co-culturing the embryonic stem cells with a human foreskin fibroblast feeder cell line.
2. The method of claim 1, wherein said human foreskin fibroblast feeder cell line is prepared by: (a) isolating foreskin cells from foreskin tissue; (b) culturing said foreskin cells in a culture medium including serum and/or serum replacement thereby preparing said human foreskin fibroblast feeder cell line.
3. The method of claim 1, wherein said embryonic stem cells are of human origin.
4. The method of claim 2, wherein said foreskin tissue is obtained from an 8-14 day old male individual.
5. The method of claim 2, wherein step (a) is effected by: (i) mincing said foreskin tissue; (ii) dissociating said foreskin tissue resultant from step (i) into single cells.
6. The method of claim 5, wherein step (ii) is effected via treatment with trypsin.
7. The method of claim 2, wherein said serum and/or said serum replacement are provided at a concentration of at least 10 %.
8. The method of claim 2, wherein said serum is human serum.
9. The method of claim 2, wherein said serum and/or said serum 32 167776/2 replacement are provided at a concentration of 15 %.
10. A cell culture comprising: (i) embryonic stem cells; and (ii) human foreskin fibroblast cells capable of maintaining said embryonic stem cells in an undifferentiated state.
11. 1 1. The cell culture of claim 10, wherein said embryonic stem cells are of human origin.
12. The cell culture of claim 10, wherein said human foreskin fibroblast cells are capable of forming a mono-layer in the cell culture.
13. The cell culture of claim 10, wherein said cell culture includes a culture medium including serum replacement and/or human serum.
14. The cell culture of claim 13, wherein said serum replacement and/or said human serum are provided at a concentration of at least 10 %.
15. The cell culture of claim 13, wherein said serum replacement and/or said human serum are provided at a concentration of 15 %.
16. The cell culture of claim 10, wherein said human foreskin fibroblast cells of said cell culture maintain said embryonic proliferative state through at least 87 passages. Patent Attorney G.E. Ehrlich (1995) Ltd. 11 Menachem Begin Street 52 521 Ramat Gan
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL167776A IL167776A (en) | 2002-10-07 | 2005-03-31 | Human foreskin cells suitable for culturing stem cells |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41624202P | 2002-10-07 | 2002-10-07 | |
| US10/368,045 US7267981B2 (en) | 2002-10-07 | 2003-02-19 | Human foreskin fibroblasts for culturing ES cells |
| PCT/IL2003/000804 WO2004031343A2 (en) | 2002-10-07 | 2003-10-07 | Human foreskin cells suitable for culturing stem cells |
| IL167776A IL167776A (en) | 2002-10-07 | 2005-03-31 | Human foreskin cells suitable for culturing stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL167776A true IL167776A (en) | 2010-04-15 |
Family
ID=42263707
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL167776A IL167776A (en) | 2002-10-07 | 2005-03-31 | Human foreskin cells suitable for culturing stem cells |
Country Status (1)
| Country | Link |
|---|---|
| IL (1) | IL167776A (en) |
-
2005
- 2005-03-31 IL IL167776A patent/IL167776A/en active IP Right Grant
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