IL156601A - Chroman compounds and pharmaceutical compositions comprising them - Google Patents

Chroman compounds and pharmaceutical compositions comprising them

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Publication number
IL156601A
IL156601A IL156601A IL15660103A IL156601A IL 156601 A IL156601 A IL 156601A IL 156601 A IL156601 A IL 156601A IL 15660103 A IL15660103 A IL 15660103A IL 156601 A IL156601 A IL 156601A
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IL
Israel
Prior art keywords
compound
reference example
methyl
oxo
piperazin
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IL156601A
Other languages
Hebrew (he)
Inventor
Marc Chapdelaine
Timothy Davenport
Markus Haeberlein
Carey Horchler
John Mccauley
Edward Pierson
Daniel Sohn
Original Assignee
Astrazeneca Ab
Marc Chapdelaine
Timothy Davenport
Markus Haeberlein
Carey Horchler
John Mccauley
Edward Pierson
Daniel Sohn
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Priority claimed from SE0103646A external-priority patent/SE0103646D0/en
Application filed by Astrazeneca Ab, Marc Chapdelaine, Timothy Davenport, Markus Haeberlein, Carey Horchler, John Mccauley, Edward Pierson, Daniel Sohn filed Critical Astrazeneca Ab
Publication of IL156601A publication Critical patent/IL156601A/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/12—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/06—Antimigraine agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/22—Anxiolytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/48—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
    • C07D215/50—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen attached in position 4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
    • C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
    • C07D233/56—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D249/00—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms
    • C07D249/02—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms not condensed with other rings
    • C07D249/08—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
    • C07D311/24—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/04—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Pain & Pain Management (AREA)
  • Psychiatry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Quinoline Compounds (AREA)
  • Pyrane Compounds (AREA)

Description

156601/3 CHROMAN COMPOUNDS AND PHARMACEUTICAL COMPOSITIONS COMPRISING THEM Field of the Invention This invention relates to novel 8-amino derivatives, pharmaceutical compositions containing them and their use in the preparation of a medicament for fee treatment of migraine.
^Background of the Invention Serotonin (5-HT) has been implicated in many psychiatric disorders inch-ding but not limited to depression, generalized anxiety, eating disorders, dementia, panic disorder, and ΰ slee disorders. Furthermore serotonin has been implicated in gaslromtestinal disorders, cardiovascular regulation, motor disorders, endocrine disorders, vasospasm and sexual dysfunction. Serotonin receptors have been subdivided into at least 14 subtypes, see Barnes and Sharp, Neuropharmacology, 1999, 38, 1083-1152, incorporated herein by reference. These various subtypes are responsible for serotonin's action in many pafeophysicogical conditions. The 5-HTj family of receptors has high affinity for serotonin and consists of five related' receptors. This family includes the 5-HTIB and 5-HTID receptor subtypes.
Compounds feat interact with fee 5-HTi family are known to have therapeutic potential in the above mentioned disorders and diseases. In particular, compounds feat are 5HTIB and 5HTm antagonist have been known to be antidepressant and anxiolytic agents. Compounds feat are ^ SHTiB and 5HTID agonists have been used in fee treatment of migraine.
Summary of the Invention Provided herein is a compound having fee formula (I): R3 is, at each position, independently represented by hydrogen, optionally substituted alkyl, optionally substituted cycloalkyi, , feiomefeoxy, -NBA, -HAa, -NHC(=0)A, aminocarbonyl, -OA, cyano or ary¾ A is optionally substituted alkyl, optionally substituted cycloalkyi, optionally rabstituted alkenyi or optionally subslitated alkynyl; represented by (i), (ii), (iii), or (iv) below: ■ (i) (ii) (iii) (iv) R3 is independently at each position represented by -H, optionally substituted C].&]ky[, optionally substituted C2-6alkenyl, optionally substituted C2-6alkynyl, optionally substituted C3.6cycloaIkyl or AOH; ' n is 2, 3 or 4;· .
P is a heterocyclic ring; • R6 is -H or methyl; Y is -C(=0)NH-, -C(=0)NA-, -C(=0)N(A)-, - HC(=0)-, -C(=S) H-, -C¾NH-,. -C(=0 , -C(=0)CH2-, -CH2C(=0)-, -C(= 0)-piperazine-, , -NAC(=0)-, -C(=S)N(A)-, CH2NA, NACH2 or -a 5-membered heterocyclic.
R7 is a monocyclic or bicyclic aromatic ring or a heterocycle optionally substituted by one or more substituents selected from R8-R9 and R10; wherein R7 is connected to Y either by a single bond or by a ring fusion; R8 is -C¾-, -C(=0)-, -S02-, - S02NH-, -C(=0)NH-, -0-, -S-, -S(O)-, a single bond as tether from R7 to R9, or a five membered heterocyclic connected to R7 by either a single bond or by ring fusion; R9 is optionally substituted heterocycle, optionally substituted aryl,- optionally substituted piperazinyl-Rl l, optionally substituted morpholinyl-Rl 1 or optionally substituted tbiomorpholinyl- or -C(=0)A; · R10 is optionally substituted alkyl, optionally substituted cycloalkyl, hydroxy, aryl, cyano, halogen, -C(=0)NH2-, methylthio, -NHA, -NA2, -1^HC(=0)AS C(=0) HA, C(=0)NA2, or . OA; .
R1 ! is -H, alkyl, AOH, -S02A, -S02NH2, -S02 HA, -S02NA2, -S02NHAR9, -C(=0)R9, -alkylR9, C(=0)A, C(=0)NH2) C(=0)NHA, C(=0)NA2 or -C(=0)OA; or a pharmaceutically acceptable salt of said compound.
The term "hydrocarbyl" refers to any structure comprising only carbon and hydrogen atoms up to 14 carbon atoms.
The term "alkyl" used alone or as a suffix or prefix, refers to straight or branched chain hydrocarbyl radicals comprising 1 to about 12 carbon atoms.
The term "alkenyl" refers to straight or branched chain hydrocarbyl radicals having at least one carbon-carbon double bond and comprising at least 2 up to about 12 carbon atoms. 5 The term "alkyhyl" refers to straight or branched chain hydrocarbyl radicals having at least one carbon-carbon triple bond and comprising at least 2 up to about 12 carbon atoms.
The term "cycloalkyl" refers to ring-containing hydrocarbyl radicals comprising at . least 3 up to about 12 carbon atoms.
The term "cycloalkenyl" refers to ring-containing hydrocarbyl radicals having at least . 0 one carbon-carbon double bond and comprising at least 3 up to about 12 carbon atoms.
The term "cycloalkynyl" refers to ring-containing hydrocarbyl radicals having at least one carbon-carbon triple bond and comprising about 7 up to about 12 carbon atoms.
The term "aromatic" refers to hydrocarbyl radicals having one or more polyunsaturated carbon rings having aromatic character, (e.g., 4n + 2 delocalized electrons) and comprising 6 up to about 14 carbon atoms.
The term "aryl" refers to aromatic radicals including both monocyclic aromatic radicals comprising 6 carbon atoms and polycyclic aromatic radicals comprising up to about • 14 carbon atoms.
The term "alkylene" refers to divalent alkyl moieties, wherein said moiety serves to link two stractures together.
The term "heterocycle" or "heterocyclic" or heterocyclic moiety" refers to ring- containing monovalent and divalent radicals, having one or more heteroatoms, independently selected from N, O and S, as part of the ring structure and comprising at least 3 and up to about 20 atoms in the rings preferably 5 and 6 membered rings. Heterocyclic moieties may be saturated or unsaturated, containing one or more double bonds, and heterocyclic moieties may contain more than one ring.
The term "heteroaryl" refers to heterocyclic monovalent and divalent radicals having aromatic character.
Heterocyclic moieties include for example monocyclic moieties such as: aziridine, oxirane, thiirane, azetidine, oxetane, thietane, pyrrolidine, pyrroline, imidazolidine, pyrazolidine, dioxolane, sulfolane 2,3-dihydrofuran, 2,5-dihydrofuran tetrahydrofuran, . thiophane, piperidine, 1,2,3, 6-tetrahydro-pyridine, piperazine, morpholine, thiomorpholine, pyran, thiopyran, 2,3-dihydropyran, tetrahydropyran, 1,4-o!ihyclropyriciine, 1,4-dioxane, 1,3- dioxane, dioxane, homopiperidine, 2,3,4,7-tetrahydro-lH-azepine homopiperazine, 1,3- dioxepane, 4,7-dihydro-l,3-dioxepin, and hexamethylene oxide. In addition heterocyclic moieties include heteroaryl rings such as: pyridyl, pyrazinyl, pyiirnidinyl, pyridazmyl, thienyl, furyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, pyrazolyl, isothiazolyl, isoxazolyl, 1,2,3-triazolyl, tetrazolyl, 1,2,3-thiadiazolyl, 1,2,3-oxadiazolyl, 1,2,4-triazolyl, 1,2,4- thiadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-triazolyl, 1,3,4-thiadiazolyl, and 1,3,4 oxadiazolyl. Additionally, heterocyclic moieties encompass polycyclic moieties such as: indole, indoline, quinoline; tetrahydroquinoline, isoquinoline, tetrahydroisoquinoline, 1,4-benzodioxan, coumarin, dihydrocoumarin, benzofuran, 2,3-d hydrobenzoniran, 1,2-benzisoxazole, benzothiophene, benzoxazole, benzthiazole, benzimidazole, benztriazole, tMoxanthine, carbazole, carboline, acridine, pyrolizidine, and quinolizidine.
In addition to the polycyclic heterocycles described above, heterocyclic moieties . include polycyclic heterocyclic moieties wherein the ring fusion between two or more rings comprises more than one bond common to both rings and more than two atoms common to both rings. Examples of such bridged heterocycles include quinuclidine, diazabicyclo[2.2.1]heptane and 7-oxabicyclo[2.2.1]heptane.
The term "halo" or "halogen" refers to fluorine, chlorine, bromine and iodine radicals. The term "alkoxy" refers to radicals of the general formula -O-R, wherein R is selected from a hydrocarbyl radical. Alkoxy moieties include methoxy, ethoxy, propoxy, isopropoxy, butoxy, t-butoxy, isobutoxy, cyclopropylmethoxy, allyloxy, andpropargyloxy..
The term amine or amino refers to radicals of the general formula -NRR', wherein R and R' are independently selected from hydrogen or a hydrocarby radical.
Detailed Description of the Invention In a further aspect of the invention, A, R1 and R3, each independently, as an alkyl, alkenyl, alkynyl and as a cycloalkyl, may optionally be substituted with halogen, nitro, cyano, hydroxy, trifluoromethyl, amino, carboxy, carboxamido, amidino, carbamoyl, mercapto, sulfamoyl, CM alkyl, C2-4 alkenyl, C2-4 alkynyl, C3-6 cycloalkyl, cycloalkenyl, C alkoxy, C alkanoyl, CM alkanoyloxy, N-(CM alkyl), N(CM alkyl)2, CM alkanoylamino, (C alkanoyl)2arnino,'N-(CM alkyl)carbamoyl, N,N-(CM alkyl)2carbamoyl, (CM)S, (CM alkyl)S(O), (C alkyl)S(0)2) (CM) alkoxycarbonyl, N-(CM alkyI)sulfamoyl, N,N-CM alkyl)sulfamoyl, Ci^ alkylsolfonylarnino, and heterocyclic.
Examples of optional substituents for aryl and heterocyclic groups, when not otherwise defined, are halogen, nitro, cyano, hydroxy, trifluoromethyl, amino, carboxy, carboxamido, amidino, carbamoyl, mercapto, sulfamoyl, CM alkyl, C2-4 alkenyl, C2- alkynyl, ' C3.6 cycloalkyl, C3.6 cycloalkenyl, CM alkoxy, CM alkanoyl, CM alkanoyloxy, N-(CM alkyl), N(CM alkyl)2, C alkanoylamino, (CM alkanoyl^amino, N-(CM alkyl)carbamoyl, N,N-(CM alkyl)2carbamoyl, (CM)S, (Cm alkyl)S(O), (CMalkyI)S(0)2, (C ) alkoxycarbonyl, N-(C alkyI)sulfamoyl, Ν,Ν-CM alkyI)sulfamoyl, CM alkylsolfonylamino, and heterocyclic.
A, R1 and R3 each independently as an alkyl, alkenyl or alkynyl may be straight or branched, preferably having 1-6 carbon atoms. A, R1 and R3 preferably have 3-6 atoms when each are independently a cyclic alkyl. Other preferable values for A, R1 and R3 when each are an alkyl include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, ferf-butyl, cyclopentyl, neopentyl and cyclohexyl. Preferable values for R1 when R1 is a halogen are fluorine, chlorine, and bromine. Other preferable values for R1 when R1 is at position 6 on the ' bicyclic ring are methyl, ethyl, ethoxy and methoxy. Preferable values for R1 when R! is at ■ position 5 on the bicyclic ring are -H, methyl, ethyl and methoxy. When R1 is at position 5- on the bicyclic ring, R1 is more preferably -H. When R1 is at position 7- on the bicyclic ring, R1 is preferably -H.
R2 is preferably represented by Formula i. Preferably R2 is represented by formula i, wherein n equals 2. Most preferably R is represented by N-methyl piperazinyl.
R3 is preferably represented by hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, and ter/-butyl. R3 is most preferably represented by methyl.
R4 is preferably represented by hydrogen, methyl, ethyl, n-propyl, isopropyl and trimethylsilanyl-ethoxymethoxy. R4 is most preferably represented by methyl.
R6 is preferably represented by H.
Y represents a linking group. Y is preferably -C(=0)N(C.¾)-, when Y is -C(=0)N(A)-. Y may also be -C(=0)-piperazine. When Y represents a five-membered heterocyclic ring, Y may be represented by, for example, pyrrole, thiophene, furan, imidazole, thiazole, oxazole, pyrazole, isothiazole, isoxazole, 1,2,3-triazole, 1,2,3-thiadiazole, 1,2,3-oxadiazole, 1,2,4-triazole, 1,2,4-thiadiazole, 1,2,4-oxadiazole, 1,3,4-triazole, 1,3,4-thiadiazole or 1,3,4-oxadiazole.
More preferably, Y is -C(=0)NH-.. \ Examples of R7 that represent monocyclic or bicyclic aromatic ring or a heterocycle include, but are not limited to, phenyl; 1- and 2-naphthyl; 2-, 3- and 4-pyridyl; 2- and 3-thienyl; 2- and 3-furyl; 1-, 2- and 3-pyrrolyl; imidazolyl; thiazolyl; oxazolyl;pyrazolyl; isothiazolyl; isoxazolyl; 1,2,3-triazolyl; 1,2,3-thiadiazolyl; 1,2,3-oxadiazolyl; 1,2,4-triazolyl; 1,2,4-thiadiazolyl; 1,2,4-oxadiazolyl; 1,3,4-triazolyl; 1,3,4-thiadiazolyl: 1,3,4 oxadiazolyl; quinolyl; isoquinolyl; indolyl; benzothienyl; berizofuryl; benzimidazolyl; benztbiazolyl; benzoxazolyl; or triazinyl.
R7 may also be represented by the Formula (v): R7 may further be represented by the Formula (vi): When the values for R7 are as set forth above, R8 may be a single bond as tether, -C(=0)-, -CH2-, -C(=0)-, -SO2-, -S(=0)-, -S-, -0-, -C(=0)NH-, -S02NH-, or a five membered heterocycle connected to R7 by single bond or by a ring fusion; and R9 may represent an aryl, heterocyclic or heteroaryl each independently optionally substituted with halogen, nitro, cyano, hydroxy, trifluoromethyl, amino, carboxy, carbamoyl, mercapto, sulfamoyl, C alkyl, C2- alkenyl, C2- alkynyl, cycloalkyl, C3-6 cycloalkenyl, C alkoxy, C alkanoyl, CM alkanoyloxy, N-(CM alkyl), N(CM alkyF , CM aDcanoylarn.no, (CM alkanoyl)2amino, N-(d. 4alkyl)carbamoyl, N,N-(CM)2carbamoyl, CM)S, CMS(0), (CMalkyl)S(=0)2, (CM) alkoxycarbonyl, N-(CM alkyl)sulfamoyl, N,N-CM alkyl)sulfamoyl,' CM alkylsolfonylamino, or heterocychc. Preferably R9 is an optionally substituted heterocyclic moiety.
More preferably R9 represents piperazine, thiomorpholine or morpholine each independently optionally substituted on carbon with at least one substituent selected from A. R8 may be a five membered heterocycle, incorporating at least one heteroatom selected from N, O, or S and it may be connected to R7 by a ring fusion, preferably when R7 is phenyl.
When R8 is a single bond as tether, R9 is preferably methoxy, cyano, a five-membered heterocycle optionally substituted with at least one substituent represented by A or Rn for example compounds represented by the Formulas (vii), (viii) and (ix): N N — R 11 (vii) When R8 is represented by a 5-membered heterocyclic comprising N and further when it is connected to R7 by a ring fusion, R9 is preferably -C(=0)A attached at the nitrogen atom. R9 is most preferably -C(=0)CH2CH3 .
When R7 is phenyl or a 6-membered heterocyclic ring, R9, is attached via the R8 tether at the 2-, 3- or 4-position of the phenyl or a 6-membered heterocycUc ring. Preferably, R9 is attached via the R8 tether at the 3- or 4-position of the phenyl or a 6-membered heterocyclic ring. More preferably, R is attached via the R tether at the 4 position of the phenyl or a 6- membered heterocyclic ring.
R10 may be represented by alkyl or cycloalkyl each independently optionally substituted with halogen, nitro, cyano, hydroxy, trifluoromethyl, amino, carboxy, carbamoyl, mercapto, sulfamoyl, CM alkyl, C2-4alkenyl, C2-4 alkynyl, C3..6 cycloalkyl, C3.6 cycloalkenyl, C alkoxy, CM alkanoyl, C alkanoyloxy, N-(CM alkyl), N(C.M alkyl)2, CM · alkanoylarnino, (CM alkanoyl)2amino, N-(CMalkyl)carbamoyl, N,N-(Ci ^carbamoyl, CM)S, CMS(O), (CMalkyl)S(0)2, (CM) alkoxycarbonyl, N-(CM aIkyI)sulfamoyl, N,N-CM alkyl)sulfamoyl, CM alkylsulfonylamino, or heterocyclic. R10 is preferably a halogen, preferably chlorine or fluorine, cyano, or -OCH3. When R10 is a halogen it is preferably chlorine or fluorine. When R7 is a phenyl or 6-membered heteroaromatic ring, R10 is attached at the 2-, 3- or 4-position of the phenyl or a 6-membered heterocyclic ring.
Preferably, R10 is attached at the 2- or 3-position of the phenyl or a 6-membered heterocyclic ring when R is attached via the R tether at the 4-position of the phenyl or a 6-membered heterocyclic ring. More preferably, R10 is attached at the 3-position of the phenyl or a 6- . membered heterocyclic ring when R9 is attached via the R8 tether at the 4-position of the phenyl or a 6-membered heterocyclic ring.
When R8 is represented by a single bond as tether, R9 is preferably represented by an optionally substituted heterocyclic, optionally substituted on carbon with at least one substituent selected from A and further substituted on a heteroatom opposite to the heteroatom attached to the tether, with a substituent represented by R" (see e.g., Formulas (vii), (viii) and (ix)). The preferred heterocyclic compounds for R8 ' are piperazine, morpholine, or thiomorpholine When 11 represents S02A it is preferably represented by an alkylsufonyl, more preferably -S02CH3, -S02CH2CH3( S02-n-C3H7, S02-i-C3H7, SO2-n-C4Hi0, -SO2-i-C4H10, or - S02-t-C4Hi . When R1 1 represent C(=0)A, it is preferably represented by an alkylcarbonyl more preferably -C(=0)C¾, -C(==0)CH2CH3, C(=O)-n-C4Hi0) -G(=O)-i-C4Hi0, -G(=0)-t- C4Hio, or -C(=0)C3H7.- When R11 is represented by C(=0)NHA or C(=0)NA2 it is preferably an alkyl or dialkyl carbamoyl more preferably or C(=0)NH-cycloC5Hio,. When R11 is represented by C(=0)R9 it is preferably -C(=0)- pyrrolidine, or -C(=0)-morpholine. When R! 1 is represented by S02NA2 it is preferably S02N(CH3)2, . When R11 is represented by AOH, it is preferably represented by, CH2CH2OH . or -C(=0)CH2CH2OH. R11 may also be represented by -C(=0)OC4Hio.
In preferred embodiments, when Y is represented by -C(=0)NH: (a) R1 is halogen or methoxy, most preferably fluorine, at the 6th position of the bicyclic ring, and is preferably hydrogen, methyl, ethyl or methoxy at the 5th position of the bicyclic ring, and is hydrogen at the 7th position on the bicyclic ring; (b) R2 is methyl piperazine; (c) R6 is hydrogen; (d) R7 is phenyl substituted with R8-R9 (e) R8 is a single bond as tether; (f) R9 is a heterocyclic moiety, preferably morpholine or piperazine attached to R8 by • nitrogen and optionally substituted on the other nitrogen (for piperazine) with R11 or optionally substituted on the oxygen with R11 when R9 is morpholine; (g) R1 1 is AOH or -S02A wherein A is represented by methyl or ethyl.
The compounds provided herein are useful in the form as a free base, but may also be provided in the form of a pharmaceutically acceptable salt, and/or in the form of a pharmaceutically acceptable hydrate. For example pharmaceutically acceptable salts of compounds of Formula I, include those derived from mineral acids such as for example: hydrochloric acid, nitric acid, phosphoric acid, sidfuric acid, hydrobromic acid, hydroiodic acid, nitrous acid, and phosphorous acid. Pharmaceutically acceptable salts may also be developed with organic acids including aliphatic mono and dicarboxylates and aromatic acids. Other pharmaceutically-acceptable salts of compounds of the present invention include for example hydrochloride, sulfate, pyrosulfate, bisulfate, bisulfite, nitrate, and phosphate.
Processes for the manufacture of the compounds of Formula I are provided hereinafter. Many of the Compounds described herein can be made by processes known in the chemical arts for the production of structurally analogous compounds.
Accordingly, the compounds of this invention may be prepared by employing procedures known in the literature starting from known compounds or readily prepared intermediates. For example, the core bicyclic, heterocyclic structure maybe made by first preparing a chromone, quinolone or quinoline. For compounds of the present invention that have Y as an amide linker, the compounds are preferably made by the general procedure for amide coupling, that is by coupling an anime with an acid hydrochloride. The amines used in the current invention if not commercially available may be made by known techniques. For example as a first step in the process of making compound of Formula L, a nitrp compound may be reduced to an amine. The nitro compound maybe a nitrophenyl compound. The resulting amines may be reacted with an acid hydrochloride Provided herein is a process for preparation of a precursor compound by reacting a compound of Formula (Via): Via (FJJ R2, R3, and R7 are as defined for Formula I unless otherwise specified and X is represented by 0), with for example a compound represented by:: R'02C "CO R' 2 , wherein R* is represented by alkyl, preferably lower alkyl (e.g., Ci-C6) most preferably methyl or ethyl, to form a precursor compound of Formula (VIb): VIb R1 is preferably fluorine, chlorine, methyl, methoxy, ethoxy or hydrogen. The Halogen is preferably Chlorine or Bromine. The reaction may be carried out in the presence of a catalyst such as tetrabultyammonium fluoride in THF. The reaction may be stirred for example at room temperature and refluxed with heat.
Further provided herein is a process for the preparation of a precursor compound comprising hydrolyzing the esters of compound (VIb) to form intermediate (Vic): Vic This reaction may be carried our for example by reacting a compound of Formula (VIb) with a base such as sodium hydroxide (aqueous). Also provided here is a process for the preparation of an intermediate by the cyclization of compound (Vic) to form intermediate (VId) Halogen O VId Intermediate compound (VId) may be formed by refiuxing a compound of Formula (Vic) with a strong acid (e.g., H2SO) and further refluxed with heat and an alkyl alcohol for example R' ΌΗ wherein R' ' is C1-C4 alkyl, preferably ethyL Also provided is a process for the preparation of an intermediate by reacting a compound of Formula (VId) with an amine of R2 in the presence of a catalyst and a base to form intermediate Formula (Vie): Vie It is further provided that a compound of Formula (VId) is reacted with a catalyst selected from the group consisting of nickel and palladium. Preferably the palladium is provided in the presence of a phosphine ligand for example 2,2'-bis( The base is preferably selected from the group consisting of potassium carbonate, sodium carbonate, cesium carbonate and triethylamine and mixtures thereof.
Further provided herein is an acid hydrochloride of a compound of Formula (Vie) which is intermediate Foimula'(VIf ): 156601/2 Vlf The intermediate Formula (Vlf) may be formed for example by heating a compound of Formula (Vie) in the presence of an acid and water (e.g., HCL/H^O) Further provided is intermediate Formula (VIg): 156601/2 Thus, it is disclosed that a leaving group is added to the ca boxylate of a compound of Formula (Vlf). L is a leaving group. This intermediate is useful in that the acid is activated to provide an electrophile. L is preferably represented by chlorine in intermediate Formula (VIg) which is prepared by reacting a compound of Formula (Vlf) with thionyl chloride (SOCl2).
Provided herein is a compound of Formula (Vlh): Vlh Methods for reacting amines with acid chlorides may be used to prepare compounds of formula I such as a compound of Formula (Vlh) For example, a memod for the preparation of (Vlh) may include reacting a compound of Formula (VIg) with H2N- 7 in the presence of DIPEA.
Alternatively, compounds of Formula (Vlh) may also be prepared by reacting a compound of Formula (Vlf) with H2N-R7 in the presence for example 1-hydroxybenzotriazole (HOBT), 0-(lH-Benzotriazol-l-yl)-N^i,N'N'-pentamethylene-uronium tetrafiuorborate (TBTU), and (dimethylammo)pyridine, preferably in that order.
Compounds of Formulas (Vie), (Vlf), and (VIg), and (Vlh) may also comprise a pharmaceutically acceptable salt of said compounds.
The compounds and processes above may also be used to prepare the chroman derivatives of Formula (I) via the saturation of the double bond (4H-chromene) in the bicyclic compound. Depending on the reduction conditions, the 4-oxo derivative may or may not be obtained.
Provided herein is a process for the preparation of a precursor compound by reacting a compound of Formula (Via): Via (R1, R2, R3, and R7 are as defined for Formula I unless otherwise specified and X is represented by O), with for example a compound represented by:: R 2C 'CO R' 2 , wherein R' is represented by alkyl, preferably lower alkyl (e.g., Ci-Ce) most preferably methyl or ethyl, to form a precursor compound of Formula (VIb): VIb R1 is preferably fluorine, chlorine, methyl, mefhoxy, ethoxy or hydrogen. The Halogen is preferably Chlorine or Bromine. The reaction may be carried out in the presence of a catalyst such as tetrabultyammonium fluoride in THF. The reaction may be stirred for example at room temperature and refluxed with heat Further provided herein is a process for the preparation of a precursor compound comprising hydrolyzing the esters of compound (VIb) to form intermediate (Vic): Vic This reaction may be carried our for example by reacting a compound of Formula (VTb) with a base such as sodium hydroxide (aqueous). Also provided here is a process for the preparation of an intermediate by the cyclization of compound (Vic) to form intermediate (VId) VId Intermediate compound (VId) may be formed by refluxing a compound of Formula (Vic) with a strong acid (e.g., H2SO) and further refiuxed with heat and an alkyl alcohol for example R"OH wherein R" is C1-C alkyl, preferably ethyl.
Also provided is a process for the preparation of an intermediate by reacting a compound of Fonnula (VId) with an amine of R2 in the presence of a catalyst and a base to form intermediate Formula (Vie); Vie It is further disclosed that a compound of Formula (VId) is reacted with a catalyst selected from the group consisting of nickel and palladium. Preferably the palladium is provided in the presence of a phosphine ligand for example 2,2'-bis(diphenylphosphino)-1,1 '-bmapthyl. The palladium may be provided as tris(dibenzylideneacetone) dipalladium. The base is preferably selected from the group consisting of potassium carbonate, sodium carbonate, cesium carbonate and triemylamine and mixtures thereof.
Further provided herein is an acid hydrochloride of a compound of Formula (Vie) which is intermediate Formula (Vlf): HCI Vlf The intermediate Formula (Vlf) may be formed for example by heating a compound of Formula (Vie) in the presence of an acid- and water (e.g., HCL/H20).
Further provided is intermediate Formula (VIg): Thus, it is disclosed that a leaving group is added to the carboxylate of a compound of Fonnula (Vlf). L is a leaving group. This intermediate is useful in that the acid is activated to provide an electrophile. L is preferably represented by chlorine in intermediate Formula (VIg) which is prepared by reacting a compound of Formula (Vlf) with thionyl chloride (SOCl2).
Provided herein is a compound of Formula (VTh): Vlh Methods for reacting amines with acid chlorides may be used to prepare compounds of formula I such as a compound of Formula (Vlh) For example, a method for the preparation of (VTh) may include reacting a compound of Formula (VIg) with ¾N-R7 in the presence of DIPEA.
Alternatively, compounds of Formula (Vlh) may also be prepared by reacting a compound of Formula (Vlf) with ¾N-R7 in the presence for example 1 - hydroxybenzotriazole (HOBT), 0-( 1 HrB enzotriazol- 1 -yl)-N,N,N'N' -pentamethylene- uronium tetrafluorborate (TBTU), and (dimemylamino)pyridineJ preferably in that order.
Compounds of Formulas (Vie), (Vlf), and (VIg), and (Vlh) may also comprise a , pharmaceutically acceptable salt of said compounds.
The compounds and processes above may also be used to prepare the chroman derivatives of Formula (I) via the saturation of the double bond (4H-chromene) in the bicyclic compound. Depending on the reduction conditions, the 4-oxo derivative may or may not be obtained.
A method for preparing the acid hydrochlorides useful in synthesis of a chromone is set forth in Scheme 1 below: A method for preparing the acid hydrochlorides useful in synthesis of a chromone is set forth in Scheme 1 below: Halogen = CI, Br (R1)q = -OCH3, F, CH3. CI, O , H. Vlb Via NaOH Vld H Vic V (cH2)n ( Or precursor amines for (ii) or (iii)1 structural variations of R2 ' Pd catalyst phosphine ligand cesium carbonate Vie Vlf Scheme 1: Preparation of chromone-2-carboxylic acids as termediates in the synthesis of compounds of the pres ent invention.
Alternatively, the chromone-2-carboxylic acid may be converted to the acid chloride and reacted immediately with an appropriate amine, as depicted in Scheme 2, below: Scheme 2. Amide synthesis via acid chloride intermediate.
Additional functional group manipulations include, but are not limited to, O-dealkylation and N-dealkylation (Scheme 3).
Quinoline and quinolone compounds of the present invention are prepared and derivatized via synthetic routes similar to those employed for synthesis of the chromone-2-carboxamides described above and in Schemes 1-3. These synthetic routes to quinoline and quinolone compounds of the present invention are depicted in Scheme 4, infra.
It will be appreciated by those skilled in the art that certain compounds of the present invention contain for example asymmetrically substituted carbon and or sulfur atoms, and accordingly may exist in and be isolated in, optically-active and racemic forms. It will be appreciated by those skilled in the art-that certain compounds of the present invention contain for example asymmetrically substituted carbon and/or sulfur atoms, and accordingly may exist in and be isolated in, optically-active -and racemic forms. Some compounds may exhibit polymorphism, thus it is to be understood that the present invention encompasses racemic, optically-active, polymorphic or stereoisomeric forms, or mixtures thereof, which forms possess properties useful in the treatment of the disorders set form below. Preparation of optically active forms is well known in the art how (for example by resolution of racemic forms by recryslallization techniques, synthesis from optically-active starting materials, chiral synthesis, or by chromatographic separation using a chiral stationary phase) and how to deteimine efficacy for the treatment of the disorder described above.
Compounds of Formula I have been found to be 5-HTIB and SHTJD agonists. The compounds of Formula I, and their pharmaceutically acceptable salts, may also be used in the treatment of migraine.
Further provided is 1he use of a compound of Formula I in the preparation of a medicament for the treatment of a disorder such as migraine in a warm-blooded animal, preferably a mammal, more preferably a human, suffering from such disorder.
The invention further provides a pharmaceutical composition suitable for the treatment of the above describe disorders comprising administering to a warm-blooded animal having such disorder an effective amount of a pharmaceutical composition of a compound of Formula I, or a pharmaceutically acceptable salt The invention also provides a pharmaceutical composition comprising a compound of Formula I, as defined herein, or a pharmaceutically acceptable salt, in combination with a pharmaceutically acceptable carrier. Preferred compounds of Formula I, for use in the compositions of the invention are as described above.
All compounds described herein demonstrate binding affinities (observed Ki values), in an assay described below, of less than about 1 ΟμΜ. Further, compounds of the present invention not only demonstrate 5HTIB antagonist activity by reversing 5HTIB agonist-induced hypothermia in the guinea pig, these compounds are considered to be orally active, and hence, they are the preferred compounds. Examples 1, 10, 11, 31, 32, 34, 44, 55, 56, 57, 71 and 72, infra, demontrate 5HT!B antagonist activity in a dosage range of 0.006-5.5 mgkg. In addition, compounds described herein demonstrate activity in the learned helplessness assay for antidepressant antianxiety activity. Examples 31, 44, 71 and 72, infra, demonstrate activity in the learned helplessness assay. In addition, compounds were tested for maximal -mtrinsic activity (IA), and were found to have measured IA's of negative 50% to positive 150% in the GTPyS assay described below, thus demonstrating a range of response from agonism (low percentages) to antagonism (high percentages).
The compounds described herein may be provided or delivered in a form suitable for oral use, for example in a tablet, lozenge, hard and soft capsule, aqueous solution, oily ■ solution, emulsion, and suspension. The compounds may be also be provided for topical administration, for example, as a cream, ointment, gel, spray, or aqueous solutions, oily solutions, emulsions or suspensions. The compounds described herein may also be provided in a form suitable for nasal administration for example, as a nasal spray, nasal drops, or dry powder. The compositions may also be administered to the vagina or rectum in the form of a suppository. The compounds described herein may also be administered parentally, for • example by intravenous, intravesicular, subcutaneous, or intramuscular injection or infusion. The compounds may be administered by insufflation (for example as a finely divided powder). The compounds may also be administered transdermally or sublingually.
The compounds of the invention may accordingly be obtained by conventional, procedures using conventional pharmaceutical excipients, well known in the art. Thus, compositions intended for oral use may contain, for example, one or more coloring, sweetening, flavoring and/or preservative agents.
The amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending uppn the host treated and the particular route of adhmiinstration. The size of the dose for therapeutic or prophylactic purposes of a compound of the Formula I, will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine. Various assays and in vivo tests are known for determining the utility of the compounds in the disorders noted above and specifically as agonists and antagonists of 5HTIB and 5HTID The utility of the compounds for example to treat depression may be shown via a learned helplessness test in guinea pigs, which is used extensively as correlative to antidepressant activity in humans. The learned helplessness test may be carried out as follows: Seventy male Hartley guinea pigs, each weighing about 350-425 gm are fed ad lib, and are housed under a 12-hour light dark cycle. The procedure consists of two phases: The induction phase and the avoidance training phase. In the induction phase, subjects are placed into standard shuttle cages (20 L X 16 W X 21 centimeters H ) which are fitted with a grid floor. Electrical stimulation (1.25 mA, 10 sec duration) is delivered to the floor of the cage every 90-sec during 1 hour daily sessions. Subjects have no opportunity to escape or to avoid shocks. Induction is conducted for 2 consecutive days.
In avoidance Iraining, testing is also conducted in the shuttle cages, except that the ' subjects are not returned to the same chamber in which induction had occurred. Additionally, all cages are fitted with a partition- with an arch in the center of the cage, through which animals can pass between the left and right halves of the cage. The procedure employed is a standard shuttle avoidance procedure in which a compound, conditioned stimulus (a 10-sec presentation bf a tone and turning on of a lamp on the side of the cage that the guinea pig was occupying) serves to indicate presentation of electrical current to the floor of the cage. Shock is presented for a 5 sec period, 5 sec after initiation of the conditioned stimulus: Entry into the opposite side of the shuttle cage via the arched partition prior to shock onset results in the end of the trial (avoidance response). If shock is delivered, entry into the opposite side of the ' cage results in termination of the shock and CS (escape). Reversal of learned helplessness in the induction subjects correlates to antidepressant activity of the test compound.
Avoidance training, 45-min in duration, is conducted on 2- consecutive days, beginning 48 hr after the final induction session. Seventy subjects are assigned to 1 of 6 groups of 11-12 animals. The groups' are as follows: 1) No induction group. The subjects are placed into the shuttle cages but are not given inescapable shock, the animals are subsequently trained in the avoidance procedure and the vehicle is administered; 2) Induction vehicle control group; 3) Imipramine 17.8 mg/kg; 4) 0.3 mg kg compounds; ) 1 mg/kg compounds; and 6) 5 mg/kg compounds.
Groups 2-6 are given induction and avoidance training sessions. Injections are adrninistered immediately following induction sessions and 1 hour prior to avoidance training ■ sessions. A second injection is administered 7-8 hours following the first injection, for a total of 9 injections administered over 5 days. No injections are administered following the final avoidance training session.
Compounds of the present invention may be administered in a volume of lmL kg bwt. Imipramine is dissolved in DI water. The compounds are dissolved in DI water, to which was added a few drops of lactic acid (pH 5.5). The vehicle control is DI water prepared with lactic acid to the same pH as the-treated groups. .
The primary dependent variable is escape failure during avoidance training. 2-way analysis of variance (ANOVA) is used to assess overall treatment effect, with Dunn's post hoc analysis used to compare the vehicle-treated group with the drug-treated groups. The no- induction group is used to gauge whether learned helplessness is established, by comparison to the vehicle treated group.
Other assays that may be used to measure for example affinity of compounds of the present invention for 5HT[B and 5HTm receptors are described in J. Med. Chem 41 : 1218- 1235, 1228 (1998) and J. Med. Chem 42:4981-5001, (1999) and incorporated by reference herein.' These assays may be used with some modifications: Frozen membrane preparations of a stably transfected Chinese hamster ovary (CHO) cell line expressing 5-HTIB receptors and 5-ΗΤ I D receptors are thawed rapidly, briefly vortexed, and diluted in assay buffer (AB) containing 50 mM Tris-HCi, 4 mM MgCl2, 4mM CaCl2, 1 mM EDTA, and adjusted to pH 7.4 with NaOH. Final protein concentrations are - 0.185 mg/ml for 5-HTIB, and 0.4 mg ml for 5-HTID membranes. Test compounds are evaluated in competition assays using [3H]-GR125743 (Amersham). The ligand concentration in both assays was 0.27nM. Kd for [3H]-GR125743 · may vary from 0.15 riM to 0.25 nM. The 5-HTIB and 5-HTjD assays are performed . simultaneously on one 96-well assay plate, one drug/compound per plate. Ten serial dilutions (1 uM to 4 pM, final concentration) of compound are prepared in DMSO from 10 mM stock solutions. Incubation mixtures are prepared in quadruplicate in 96-deep well assay plates (Matrix 1 ml). Final assay volumes per well are 10 μΐ compound/nonspecific; 100 μΐ membranes; 100 μΐ [3HJ-GR125743; and 790 μΐ AB. Specific bmding is defined by using 10 uM Methiothepine. The assay plates are shaken for 5 rhin., and then incubated for an additional 55 min. Then the assay plates are filtered through Beckman GF B filters (soaked > ' 2 hrs. in PEI) using a Packard Filtermate 196. Filters are washed 2x with 1 ml ice-cold wash . buffer (5 mM Tris-HCl - pH7.4 with NaOH). After the filters are dried, 35 μΐ of Microscint20 is added to each well. The plates are then counted on a Packard TopCount to determine CPM's per well. Ki values are determined for each test compound utilizing the graphic and analytical software package, GraphPad Prism. Compounds are then ranked in order of potency, and selectivity. for 5-HTIB over 5-HT!D receptors.
A method that may be used to determine a compound's affinity for 5-HTiB and 5HTiD receptors is a guinea pig cortical test. This assay is described in detail by Roberts, et al, Br. J. Pharmacol., 1996, 117, 384-388, which is incorporated by reference herein. The test is carried out as follows: Guinea pigs are decapitated and the cortici is dissected out, weighed and homogenized in 50 mM Tris-HCl, pH 7.7 with an Ultra- Turrax followed by centrifugation for 10 min at 48000 x g and 5°C. The pellet is resuspended and recentrifuged. The final pellet is suspended in 0.32 M sucrose buffer to a concentration of 0.5g original wet weight per mL and stored frozen at -70°C. The radioligand binding assay is carried out as follows: [3H]GR125743 saturation studies are tested in duplicate with 3^4 mg w.w. per tube in 5 mL buffer (50 mM Tris, 4 mM CaC12, 4 mM MgC12 and 1 mM EDTA at pH 7.7), and a concentration range of 0.012 - 2 nM (10-12 concentrations) for the radioligand. Non-specific binding is detennined in the presence of 10 mM methiothepin. In competition experiments 4- 8 mg w.w. per tube and a radioligand concentration of 0.2 nM are used with 10 -12 concentrations of the competing drug. The assays are run for 2-4 hours at 30°C and terminated by rapid filtration through Whatman- GF/B filters (pretreated with 0.1% polyethyleneimine) using a Brandel cell harvester. Bovine serum albumin (0.1%) is added to the washing buffer to reduce non-specific binding. Data from the experiments may be analyzed using the iterative non-linear curve-fitting program LIGAND. The Kd values obtained from the saturation studies are used in the calculation of the Ki values by the LIGAND program. The Kd value of [3H]GR125743 may result in a measurement of 46 ± 4 pM and the Bmax in a measurement of 4.9 ± 0.2 pmol/g w.w.
A GTPyS binding assay may used to determine whether a compound is a 5HTIB or 5HTID agonist or antagonist. One assay available measures agonist stimulated GTP binding for example as set forth by Lazareno, S. (1 99) Methods in Molecular Biology 106: 231 -245. Membrane preparations of a stably transfected CHO cell line expressing human 5-HTIB receptors are purchased for example from Unisyn, Hopkinton, MA. Frozen membranes are ' thawed, briefly sonicated, and diluted to i67 /ml protein in assay buffer containing 20 mM HEPES, 100 mM NaCl, ImM MgCL2 and ΙμΜ GDP, pH adjusted to 7.4 with NaOH.
Diluted membranes are briefly homogenized with a Polytron and allowed to equilibrate at room temperature for at least 15 rninutes before use. Serial dilutions (10 μΜ to 1 M, final concentration) of test compounds are prepared in buffer with and without 100 nM 5-HT (final concentration) from 10 mM DMSO stock solutions. Incubation mixtures are prepared in · quadruplicate in 96-well, deep-well plates and consisted of 180 μΐ. of membranes (30 μ protein) and 40 fiL of compound with or without 5-HT. After an incubation period of 15 minutes at room temperature, 20 μΐ, of [35S]GTFyS (NEN; 100 pM final concentration) is added to begin the assay. Mixtures are shaken for 2 minutes and incubated at room temperature for an additional 28 minutes. The reaction is stopped by rapid filtration through Beckman GF/B glass fiber filters using a 96-well Packard cell harvester. Filters are washed four times with 1 mL ice-cold water. The filter plates are nominally dried and 30 pL of scintillation cocktail (MicroScint 40, Packard) is added to each well. CPMs for each well is determined using a TopCount Scintillation Counter (Packard). Maximum stimulation of [35S]GTPyS binding is defined in the presence of lOOnM 5-HT. Basal [35S]GTPTS binding is defined in buffer alone. IC50 values are defined as the concentration of compound at which 50% of the lOOnM 5-HT response [was] obtained. Maximal mtrinsic activity (IA) of a compound is defined as the percent maximal 5-HT-induced stimulation by 10 μΜ compound in the absence of 5-HT. As an inter-assay standard, a concentration response curve of 5-HT (1 μΜ to IpM final) in the absence of compounds was included in each assay and an EC50 was deten ined.
Preferred compounds of the present invention include, but are not limited to, the following compositions listed in Table 1 on the following pages.
Tabie 1: Compounds, Also provided herein are the pharmaceutically acceptable salts of the compounds set forth in Table 1.
The following reference examples illustrate the making of intermediates in the synthesis of the compounds of the present invention, and are not intend to limit the invention in any manner.
Reference Example 1 Preparation of Reference Example 1: 8-(4-Methyl-piperazin-l-yI)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride.
Reference Example la: (EJZ)-2-(2-Bromo-phenoxy)-but-2-enedioic acid diethyl ester.
Diethyl acetyl enedicarboxylate (20 ml, 0.162 mol) was added to 2-bromophenol (2? g, 0 0.162 mol), in anhydrous 2-propanol (60 ml) followed by the addition of a catalytic amount of tetrabutylammonium fluoride (0.5 ml, 1.0 M in THF). The solution was stirred at room temperature, four hours and was then heated to reflux for one hour. The mixture was cooled to ■ room temperature, then concentrated under vacuum to an oil (51 g = 1 %).
Reference Example lb: (E,Z)-2-(2-Bromo-phenoxy)-but-2-enedioic acid.
(E,Z)-2-(2-Bromo-phenoxy)-but-2-enedioic acid diethyl ester (51 g, 148 mmol) as prepared in Reference Example la was suspended in ethanol (95 ml) and a solution of sodium hydroxide ( 12.9 g, 0.323 mol) in water (95 ml) was added. The solution was refluxed for 1 h to give a clear orange solution. The mixture was cooled to room temperature and acidified with 6 M HC1 (50 ml). The mixture was then concentrated under vacuum and the residue 0 azeotroped (4x) with ethanol. The solid was filtered, washed with water and dried to give (2Z)-2-(2-bromo-4-methoxyphenoxy)-2-butenedioic acid as a light orange solid (24.3 g, 88 % yield). This crude product was used without further purification.
Reference Example lc: Ethyl-8-Bromo-4-oxo-4H-chromene-2-carboxylate. , Sulfuric acid (95mL) was added to crude (E,Z)-2-(2-Bromo-phenoxy)-but-2-enedioic acid as prepared in Reference Example lb. After heating the mixture with a heat gun for 45 min an orange milky solution was obtained. This solution was slowly added to refluxing absolute ethanol (500 mL). After the addition, the reaction was refluxed for 30 min.then allowed to cool. Crystals started to form after 20 rnin and the reaction was put in the refrigerator overnight. The solid was filtered, washed with cold ethanol/ water 9: 1 and dried to give ethyl 8-bromo-4-oxo-4H-chromene-2-carboxylate as an off-white solid (11.7 g, 24 % yield, mp 124-126 °C).
Reference Example Id: Ethyl-8-(4-Methyl-piperazin-l -yl)-4-oxo-4H-chromenec-2- carboxylic acid. - Ethyl •8-bromo-4-oxo-4H-chromene-2-carboxylate as prepared in Reference Example lc (Davies, Stephen et al., J. Chem. Soc. Perkin Trans I p 2597, 1 87) (3.0 g, 10.1 mmol) was azeotroped with anhydrous toluene then the white solid was dissolved in 100 mL anhydrous toluene and transferred to the reaction vessel. The. mixture was subjected to vacuum / argon (x2) and the following were added in order (positive argon pressure): N-methylpiperazine (1.3 ml, 11.1 mmol), 2,2'-bis (diphenylphosphino)-l,l '-binaphthyl (0.75 g, 1.2 mmol,), tris(dibenzylideneacetone) dipalladium (0) (0.48 g, 0.5 mmol) then cesium carbonate (4.6 g, 14.1 mmol).The mixture was again subjected to vacuum / argon and was heated at 80 °C overnight.
The cooled reaction mixture was filtered through diatomaceous earth and the toluene solution was applied directly to a 600 ml filter funnel (silica 230 - 400 mesh AST packed in ethyl acetate) and then washed with ethyl acetate (2 1). The product was eluted with 5-8 % methanol / chloroform and the desired was collected to give 2.5 g of a slightly impure orange yellow solid (mp 120-123 °C). The impure product was chromato graphed on a Waters Delta Prep 4000 using 1 PrepPak cartridge (Porasil 37-55um 125A) eluting with 3-5 % methanol / chloroform. The product was collected and dried to give ethyl 8-(4-methyl-l-piperazinyl)-4- oxo-4H-chromene-2-carboxylate as a yellow solid (2.25 g, 70 % yield mp 124-125 °C).
GC/MS (EI, M+) m/z 316.
Reference Example le: 8-(4-methyl-l-piperazinyl)-4-oxo-4H-chromene-2-carboxyhc acid hydrochloride.
Ethyl 8-(4-methyl-l-piperazinyl)-4-0xo-4H-chromene-2-carboxylate as prepared in Reference Example Id (1.01 g. 3.19 mmol) was suspended in 6 M HQ (60 ml) and to reflux for 1.5 h (after 20 min a clear solution was obtained).
The reaction was allowed to cool. The solution was concentrated in -vacuo and anhydrous toluene was added (x3) and the solution was again concentrated in vacuo to give 8-(4-methyl-l-piperazinyI)-4-oxo-4H-chromene-2-carboxyIic acid hydrochloride as a yellow powder (1.02 g, quantitative yield). LC/MS (M+l ) m Iz 289.
Reference Example 2 Preparation of 6-Methoxy-8-(4-MethyI-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxyUc acid hydrochloride.
Reference Example 2a: Diethyl (2Z)-2-(2-bfomo-4-methoxyphenoxy)-2-butenedioate.
Ethyl acetylenedicarboxylate (17.8 ml, 0.145 mol) was added to 2-bromo-4- methoxyphenol (Synlett l241, 1997) (27.3 g, 0.134 mol), in anhydrous 2-propanol (55 ml) followed by the addition of a catalytic amount of tetrabutylammonium fluoride (0.4 ml, 1.0 M in THF). The solution was stirred at room temperature overnight and was then heated to reflux for 30 min. Upon cooling a precipitate formed. The solution was cooled and filtered to give diethyl (2Z)-2-(2-bromo-4-methoxyphenoxy)-2-butenedioate as a yellow solid (29.9 g, 62 % yield). Note: the solid contains 10 % of diethyl (2E)-2-(2-bromo-4-methoxyphenoxy)-2- butenedioate. GC/MS (EI, M+) m/z 344 and 346.
Reference Example 2b : (2Z)-2-(2-bromo-4-methoxyphenoxy)-2-butenedioic acid.
Diethyl (2Zj-2-(2-bromo-4-methoxyphenoxy)-2-butenedioate (29.9 g, 86.6 mmol) as prepared in Reference Example 2a was suspended in ethanol (55 ml) and a solution of sodium hydroxide ( 7.0 g, 0.175 mol) in water (55 ml) was added. The solution was refluxed for 1 h to give a clear orange solution. Most of the ethanol was removed in vacuo then 6 M HC1 (50 ml) was added. The solid was filtered, washed with water and dried to give (2Z)-2-(2-bromo-4-methoxyphenoxy)-2-butenedioic acid as a light orange solid (24.3 g, 88 % yield).
Reference Example 2c: Ethyl-6-methoxy-8-bromo-4-oxo-4H-chromene-2-carboxylate. ■ Sulfuric acid (50ml) was added to (2Z)-2-(2-bromo-4-methoxyphenoxy)-2-butenedioic acid (24.3g, 86.6 mmol; as prepared in Reference Example 2b above). After heating the mixture with a heat gun for 5-10 min a clear deep brown solution was obtained. This solution was slowly added to refluxing absolute ethanol (250 ml). After the addition the reaction was refluxed for 30 min then allowed to cool. Crystals started to form after 20 min and the reaction was put in the refrigerator overnight. The solid was filtered, washed with cold ethanol/ water 9: 1 and dried to give etbyl 8-bromo-6-methoxy-4-oxo-4H-chromene-2-carboxyIate as an off- white solid (12.3 g, 50 % yield, mp 159-161 °C).
Reference Exampie 2d: Ethyl-6-methoxy-8-(4-Methy]-piperazin- 1 -yI)-4-oxo-4H-chromene- 2-carboxylate.
Ethyl 8-bromo-4-oxo-4H-chromene-2-carboxylate (9.2 g, 28.1 mmol), as prepared in Example 2c above, was azeotroped with anhydrous toluene then the white solid was dissolved in 300 ml anhydrous toluene in a 500 mL single-rieck round bottom flask.. The mixture was degassed by alternating argon sparge and vacuum (3x), and the following were added in order: N-methylpiperazine (4.0 ml, 35.1 mmol), 2,2'-bis (diphenylphosphino)-l, -binaphthyl (1.05 g, 1.69 mmol,), tris(dibenzylideneacetone) dipalladium (0) (0.50 g, 0.56 mmol) then cesium carbonate (12.8 g, 39.3 mmol).The mixture was again degassed via alternating argon sparge and vacuum and was heated at 80 °C for 17 h. Additional tris(dibenzylideneacetone) · dipalladium (0) (0.10 g, 0.11 mmol) and 2,2'-bis (diphenylphosphino)-l,r-binaphthyl (0.20 g, 0.32 mmol,) was added and the reaction was stirred at 80 °C for another 55 h at which time the conversion was essentially complete.
The cooled reaction mixture was diluted with tetrahydrofuran (250 mL), filtered and . concentrated under vacuum. The residue was purified by chromatography on a silica column eluted with 2-5 % methanol / chloroform and the desired fractions were collected and concentrated under vacuum and the residue triturated with methylene chloride to give 7.4 g (76%) of a yellow powder.
Reference Example 2e: 6-Methoxy-8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid.
Ethyl-6-methoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene -2-carboxylate (1.0 g. 2.89 mmol), as prepared in Reference Example 2d above, was suspended in 6 M HC1 (60 ml) and methanol (lO mL) and warmed to reflux for 3.0 h. The reaction was allowed to cool. The solution was concentrated in vacuo and anhydrous toluene was added (x3) and the solution was again concentrated in vacuo. The residue was dried under vacuum (17 h) to yield 6-methoxy-8-(4-methyl-i-piperazinyl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride as a yellow powder (1.0 g, quantitative yield).
Reference Example 3 6-Fluoro-8-(4-methyI-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxyIic acid hydrochloride.
Reference Example 3a: Diethyl (EZ)-2-(2-bromo-4-fluorophenoxy)-2-butenedioate.
This compound was synthesized from 2-bromo-4-fluorophenol and diethylacetylenedicarboxylate, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example la above.
Reference Example 3b: (EZ)-2-(2-Bromo-4-fluorophenoxy)-2-butenedioic acid.
This compound was synthesized from diethyl (EZ)-2-(2-bromo-4-fluorophenoxy)-2- butenedioate, as prepared in Reference Example '3 a above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lb above. Reference Example 3c: Ethyl-6-fluoro-8-bromo-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from (EZ)-2-(2-bromo-4-fluorophenoxy)-2-butenedioic acid, as prepared in Reference Example 3b above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lc above. Reference Example 3d: Ethyl-6-fl'uor0-.8-(4-Methyl-piperazin- 1 -yl)^4-oxo-4H-chromene-2 carboxylate.
This compound was synthesized from ethyl-6-fluoro-8-bromo-4-oxo-4H-chromene-2 carboxylate, as prepared in Reference Example 3c above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example Id above. Reference Example 3e: 6-Fluoro-8-(4-memyl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride.
This compound was synthesized starting from ethyl-6-methoxy-8-(4-Methyl-piperazii)-l-yl)-4-oxo-4H-chromene-2 -carboxylate, as prepared in Example 3d, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Exampl le above.
Reference Example 4 Preparation 6-Methyl-8-(4-methy]-piperazm-l-yI)-4-oxo-4H-chromene-2-carboxyIic acid hydrochloride.
Reference Example 4a: Diethyl (E,Z)-2-(2-bromo-4-methylphenoxy)-2-butenedioate. 2-Bromo-4-methyl phenol (10 mL, 83mmol) was dissolved in diethyl ether (90 mL). To this was added dropwise triethyl amine (13.7 mL, 98mmol) followed by dimethyl acetylene dicarboxylate (11.2 mL, 91mmol). The resulting mixture was stirred overnight at room temperature. The reaction was worked up by adding diethyl ether (200 mL) and tetrahydrofuran (50 mL) and washing the resulting mixture with IN HCl (200 mL), water (200 mL) and brine (100 mL). The organic phase was then dried. (Na2S04), filtered and concentrated to a red-brown oil which was used without further purification.
Reference 4b: (2E,Z)-2-(2-Bromo-4-fluorophenoxy)-2-butenedioic acid.
This compound was synthesized from diethyl (E,Z)-2-(2-bromo-4-methylphenoxy)-2-butenedioate,. as prepared in Reference Example 4a above, using the same synthetic procedures and the same stoichiometry as demonstrated in Example lb above.
Reference Example 4c: Ethyl-6-methyl-8-bromo-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from (2Z)-2-(2-bromo-4-methylphenoxy)-2-butenedioic acid, as prepared in Reference Example 4b above, and using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lc above. Reference Example 4d: Ethyl-6-methyl-8-(4-Methyl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from ethyl-6-methyl-8-bromo-4-oxo-4H-chromene-2-carboxylate, as prepared in Reference Example 4c above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example Id above.
Reference Example 4e: 6-Methyl-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2- carboxylic acid hydrochloride.
This compound was synthesized starting with ethyl-6-methyl-8-(4-Methyl-piperazin- 1 -yl -4-oxo-4H-chromene-2-carboxylate, as prepared in Reference Example 4d, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Exampl ■ le above.
Reference Example 5 Preparation of acid hydrochloride.
Reference Example 5a: Diethyl (E,Z)-2-(2-bromo-4-chlorophenoxy)-2-butenedioate.
This compound was prepared from 2-bromo-4-chloro phenol and dimethyl acetylenedicarboxylate by the same synthetic procedures and in the same stoichiometry as the preparation described in Reference Example 4a.
Reference Example 5b; (2E,Z)-2-(2-Bromo-4-chlorophenoxy)-2-butenedioic acid..
This compound was synthesized from diethyl (E,Z)-2-(2-bromo-4-chlorophenoxy)-2-butenedioate, as prepared in Reference Example 5a above, as using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lb above. Reference Example 5c: Ethyl-6-chloro-8-bromo-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from (2E',Z)-2 (2-bromo-4-chlorophenoxy)-2-butenedioic acid, as prepared in Reference Example 5b above, using the same synthetic procedures and the same stoichiometry as . demonstrated in Example lc above.
Reference Example 5d: Emyl-6-cWoro-8-(4-Memyl-piperazm-l*yl)-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from ethyl-6-chloro-8-bromo-4-oxo-4H-chromene-2- carboxylate, as prepared in Reference Example 5c above, using the same synthetic procedures and the same stoichiometry as demonstrated in Example Id above.
Reference Example 5e: 6-Chloro-8-(4-methyl-piperazin-l -yl)-4-oxo-4H-chromene-2- carboxylic acid hydrochloride.
This compound was synthesized starting with ethyl-6-chloro-8-(4-methyl-piperazm-l- yI)-4-oxo-4H-chromene-2-carboxylate, prepared in Reference Example 5d above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example 1 e above.
Reference Example 6 Preparation of 5-Methyl-8-(4-methyI-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxyIic acid hydrochloride Reference Example 6a: Diethyl (E,Z)-2-(2-chloro-5-methylphenoxy)-2-butenedioate.
This compound was prepared from 2-chloro-5-methylphenol and dimethyl acetylenedicarboxylate by the same synthetic procedures and in the same stoichiometry as the. preparation described in Reference Example la.
Reference Example 6b: (2E,Z)-2-(2-chloro-5-methylphenoxy)-2-butenedioic acid.
This compound was synthesized from diethyl (E,Z)-2-(2-chloro-5-methylphenoxy)-2-butenedioate, as prepared in Reference Example 6a above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lb above.
Reference Example 6c: Ethyl-5-methyl-8-chloro-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from (2Z)-2-(2-chloro-5-methylphenoxy)-2-butenedioic acid, as prepared in Reference example 6b, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example 1 c above.
Reference Example 6d: Ethyr-5-me&yl-8-(4-Methyl-piperazm-l-yI)-4-oxo-4H-chromene-2- carboxylate.
Ethyl 5-methyl-8-chloro-4-oxo-4H-chromene-2-carboxylate (1.0 g, 3.6 mmol) as prepared in Reference Example 6c above, was azeotroped with anhydrous toluene then the white solid was dissolved in 100 ml anhydrous toluene in' a 250 mL single-neck round bottom flask.;! The mixture was degassed by alternating argon sparge and vacuum (3x), and the following were added' in order: N-methylpiperazine (0.6'ml, 5.37 mmol), (2'- dicyclohexylphosphanyl-bip'henyl-2-yl)-dimethyi-amine (JACS 1998, 120, p9722) (40 mg, 0.1 mmol,), tris(dibenzylideneacetone) dipalladium (0) (66 mg, 0.072 mmol) then cesium carbonate (1.6 g, 5.37 mmol).The mixture was again degassed via alternating argon sparge and vacuum and was heated at 80 °C for 17 h. Additional tris(dibenzylideneacetone) dipalladium (0) (66 mg, 0.072 mmol) and (2'-dicyclopentylphosphanyl-biphenyl-2-yl)- dimethyl-amine (40 g, 0.1 mmol,) were added and the reaction was stirred at 80 °C for another four days at which time the conversion was still only about 50% complete by HPLC. Tetrahydrofuran (100 mL) was added, and the combined mixture was filtered, concentrated under vacuum and purified by chromatography on silica eluted with 2.5% methanol in chloroform. The desired fractions were concentrated under vacuum to yield a yellow powder (250 mg = 21%).
Reference Example 6e: 5-Methyl-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride.
This compound was synthesized starting with ethyl-5-methyl-8-(4-methyl-piperazin-l- . yl)-4-oxo-4H-chromene-2-carboxylate, as prepared in Reference Example 6d, and using the same synthetic procedures and the same stoichiometry as demonstrated in Example le above. Reference Example 7 Preparation of 5-Methoxy-8-(4-methyI-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxyIic acid hydrochloride.
Reference Example 7a: (E.,Z)-2-(2-Bromo-5-methoxyphenoxy)-2-butenedioate.
This compound was prepared from 2-bromo-5-methoxyphenol and dimethyl acetylenedicarbbxylate by the. same synthetic procedures and in the same stoichiometry as the preparation described in Reference Example la.
Reference Example 7b: (E,Z)-2-(2-Bromo-5-methoxyphenoxy)-2-butenedioic acid.
This compound was synthesized from diethyl (E,Z)-2-(2-bromo-5-methoxyphenoxy)- 2-bute edioate, as prepared in Reference Example 7a, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lb above.
Reference Example 7c: Ethyl-5-methoxy-8-bromo-4-oxo-4H-chromene-2-carboxylate.
This compound was synthesized from (E,Z)-2-(2-bromo-5-methoxyphenoxy)-2-butenedioic acid, as prepared in Reference Example 7b above, using the same synthetic procedures and the same stoichiometry as demonstrated in Reference Example lc above.- Reference Example 7d: Ethyl-5-methoxy-8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene- 2-carboxylate.
This compound was synthesized from ethyl-5-methoxy-8-bromo-4-oxo-4H-chromene- 2-carboxylate, as prepared in Reference Example 7c above, using the same synthetic procedures and the same stoichiometry as demonstrated in, Reference Example Id above. Reference Example 7e: 5-Methoxy-8-(4-methyl-piperazin-l-yl)-4-0xo-4H-chromene-2- carboxylic acid hydrochloride.
This compound was prepared from emyl-5-methoxy-8-(4-Methyl-piperazin-l-yl)-4- oxo-4H-chromene-2-carboxylate, as prepared in Reference Example 7d above, using the same method as the preparation in le.
Reference Example 8 Preparation of l-(6-Piperazin-l-yI-2,3-dihydro-indol-l-yI)-ethanone Reference Example 8a: 1 -[5-(4-Benzyl-piperazin- 1 -yl)-2,3-dihydro-indol- 1 -yl]-ethanone. l-acetyl-5-bromoindoline (3.0 g, 12.5mmol) was dissolved in toluene (60 mL). To this was added, sodium t-butoxide (1.68 g, 17.5010101), N-benzylpiperazine (2.4 mL, 13.8mmol), S-BINAP (0.93. g, 1.5mmol) and Pd2(dba)3 (0.46 g, 0.5mmol). The mixture was degassed via three cycles of vacuum and nitrogen sparge and then stirred at 95°C until GC analysis confirmed that the reaction was complete (l'h). The mixture was diluted with ethyl acetate (150 mL), washed with water and extracted with 2N HC1 (2 x 100 mL). The combined aqueous extract was basified with concentrated ammonium hydroxide and extracted with ethyl acetate (2 x 100 mL). The combined organic extract was dried (MgS04) and concentrated to yield a solid (2.7 g) which was purified by chromatography to yield a white ■ solid (1.81 g, 43%). p = 150.5-152.8°C.
Reference Example 8b: l-(6-Piperazin-l-yl-2,3-dihydro-indol-l-yl)-ethanone. l-[5-(4-Berizyl-piperazm-l-yl)-2,3-dihydro-mdol-l-yl]-e1hanone (0.37 g, l.lmmol), as prepared in Reference Example 8a above, was dissolved in methanol (5 mL). Pd/C (90 mg, 10%) and ammonium formate (0.9 g, 14mmol) was added and the resulting mixture was heated to 65°C for two hours. The mixture was filtered and the filter cake washed with hot methanol. The combined filtrate was concentrated to yield the desired product (0.26 g, 90%). Reference Example 9 Preparation of 2-chloro-5-piperazin-l-yI benzonitrile.
Reference Example 9a: 3-Cyano-4-chloroaniline. 2- Chloro-5-nitrobenzonitrile (25 g, 137mmol) was dissolved in ethanol (275 mL). Stannous chloride dihydrate (154.5 g, .685 M) was added and the mixture stirred at 70°C for min. The mixture was then cooled to room temperature and poured into crushed ice. The mixture was made basic with solid sodium hydroxide. This mixture was extracted with ethyl acetate (3 x 100 mL). The extracts were combined, washed with brine, dried (MgS0 ), concentrated and the residue dried under vacuum and recrystallized from ethanol to. yield light brown needles (10.6 g, 51%).
Reference Example 9b: 2-chloro-5-piperazin-l-yl benzonitrile. 3- Cyano-4-chloroanihne (10.1 g, 66mmol), as prepared in Reference Example 9a, was dissolved in n-butanol (300 mL) bis-(2-chloroemyl)amine hydrochloride (23.2 g, 130mmol) and potassium iodide (50 mg, catalytic) were added. The mixture was heated at reflux for three days, then cooled in a refrigerator overnight. A solid precipitate was collected by filtration, washed with cold n-butanol and dried. The crude product was distributed between methylene chloride and 2N ammonium hydroxide. The organic layer was separated, dried (Na2SC>4) and concentrated to yield a hght yellow solid (9.1 g, 59%) which gave a single peak by GC and TLC analysis.
Reference Example 10 Preparation of 4-[l>2,3]thiadiazoI-5-yl-phenylamine.
SnCl2 ' H20 (3.21 g, 5 eq) was added to a slurry of (5-(4-Nitrophenyl)-l',2,3- thiadiazole (Lancaster Synthesis) (0.59 g, 2.8 mmol) in absolute EtOH (50 mL) and the reaction heated to 70° C for 2 h. The reaction was allowed to cool to room temperature and 10 pour into saturated NaHCC^ and ice. The product was extracted with EtOAc (2X) the solution dried (MgSO^) and evaporated to dryness i vacuo to yield 0.47 g of a light yellow ■ solid mp 126-128° C.
Reference Example 11 Preparation of l-[4-(4-Amino-phenyI)-piperazin-l-yl]-ethanone.
Reference Example 11a: 4-(4-Nitrophenyl)-l-acetylpiperazine. l-(4-NitrophenyI)piperazine (2.5 g, 12.1 mmol) was dissolved in dichloromethane (100, ml). Triemylamine (2,0 ml, 14.5 mmol) was added and the reaction was cooled to 0 °C.
Acetic anhydride (1.25 ml, 13.3. mmol) was added dropwise and the reaction was stirred at 0 20 °C for 1 h. Saturated sodium bicarbonate was added and the reaction was extracted (x3) with dichloromethane, dried (MgS04), filtered and concentrated in vacuo to give 4-(4- nitrophenyl)-l-acetylpiperazine as a yellow solid (3.01 g,).GC MS (EI, M+) m/z = 249.
Reference Example lib: l-[4-(4-Ainino-phenyl)-piperazin-l-yl]-ethanone. 4-(4-Nitrophenyl)-l-acetyIpiperazine (3.0 g, 12.0 mmol), as prepared in Reference Example 25 11a above, was mixed in methanol (100 ml) and 2 M ammonia in methanol (50 ml) and 10 % palladium on carbon (300 mg) was added. The mixture was hydrogenated on a Paar apparatus (50 psi) for 1.5 h.
The reaction was allowed to cool, the catalyst was filtered and the solution was concentrated in vacuo.. The crude solid was recrystaliized from ethyl acetate to give 4-(4- acetyl- l-pipei^myl)benzenarnine as a light purple solid (1.86 g, 70 % yield, mp 149.5-150.5 °C). GC/MS (EI, M+) m/z = 219 Reference Example 12 Preparation of 4-(4-methanesulfonyl-piperazin-l-yI)-phenylamine Reference 12a: 4-(4-Nitrophenyl)-l-methylsulfonylpiperazine. 1 -(4-Nitrophenyl)piperazine (2.79 g, 13.5 mmol) was dissolved in dichloromethane (100 ml). Triemylamine (2.25 ml, 16.2 mmol) was added and the reaction was cooled to 0 °C. Methanesulfonyl chloride (1.15 ml, 14.9 mmol) was added dropwise and the reaction was stirred at 0 °C for 1 h. Saturated sodium bicarbonate was added and the reaction was extracted (x3) with dichloromethane, dried (MgS04), filtered and concentrated in vacuo to give 4-(4-nitrophenyl)-l-methylsulfonylpiperazine as a yellow solid (3.83 g, quantitative yield). GC S (EI, M+) m/z = 285.
Reference Example 12b: 4-(4-methanesulfonyl-piperazin-l -yl)-phenylamine. 4-(4-Nitrophenyl)-l-methylsulfonylpiperazine (3.83 g, 13.4 mmol), as prepared in Reference Example 12a above, was mixed in methanol (100 ml)' and 2 M ammonia in methanol (50 ml) and 10 % palladium on carbon (400 mg) was added. The mixture was hydrogenated on a Paar apparatus (50 psi) for 3 h.
The reaction was allowed to cool, the catalyst was filtered, washed with methanol then washed with chloroform. The chloroform portion contained a minor amount of the desired but looked purer. The chloroform portion was concentrated in vaciio and was recrystallized ethyl acetate to give 4-[4-(memylsulfonyl)-l-piperazmyl]benzenainine as a shiny brown solid (0.94 g, 27 % yield, mp 192-193 °C). GC/MS (EI, M+) m z = 255.
Reference Example 13 Preparation of 4-Thiomorpholin-4-yl-phenylamine: Reference Example 13 a: 4-(4-Nitro-phenyl)-thiomorpholine. 4-Fluoronitrobenzene (3.0 g, 21.3 mmol) was dissolved in toluene (25 mL). Tbiomorpholine (2.4 mL, 23.4 mmol) was added and the mixture stirred overnight at 100 °C. At 17 h, the mixture was distributed between ethyl acetate (100 mL) and saturated sodium bicarbonate (50 mL). The organic layer was separated, dried (Na2S04), filtered and concentrated under vacuum. The residue was triturated with hexane to yield a bright yellow solid.
Reference Example 13b: 4-Thiomorphohn- -yl-phenylarnine. 4-(4-Nitro-phenyl)-tiiiomorpholine(3.0g) 13.4 mmol), as prepared in Reference Example 13a above, was dissolved in ethanol (250 mL) and 10% palladium on carbon (250 nig) was added. This mixture was shaken on a Parr hydrogenator for 3 h. The reaction mixture was then filtered through diatomaceous earth and concentrated under vacuum. The residue was triturated with' hexane to yield an gray solid (2.1 g).
Reference Example 14 Preparation of l-(4-Amino-phenyl)-l-morpholin-4-yI-methanone.
Reference Example 14a: l-Moφholin-4-yl-l-(4-nitro- henyl)-methanone: 4-Nitrobenzoyl chloride (5 g, 27 mmol) in tetrahydrofuran (10 mL) was added slowly to a solution of morpholine (5g, 88 mmol) and triemylamine (2.7 g, 27 mmol) in tetrahydrofuran (50 mL), and stirred at room temperature for four hours. Ethyl acetate (200 mL) was added to the mixture and the combined mixture was washed with water (25 mL), IN HC1 (25 mL), water (25 mL), saturated sodium bicarbonate (25 mL), water (25 mL) and brine (25 mL). The mixture was dried (Na2S04), filtered and concentrated under vacuum and the residue used without further purification. ■ .
Reference Example 14b: This compound was prepared from l-morpholin-4-yI-l-(4-nitro-phenyI)-methanone as prepared in Reference Example 13b.
Reference Example 15 Preparation of 5-Arm^o-2-morphoIin-4-yI-benzonitrile Reference Example 15a: 2-Μοφ^1ώ-4^1-5-^^ο^εΓ.ζοώίπ1ε. 3-Cyano-4-fluoromtrobenzene (3.3 g, 1 .9 mmol) was dissolved in ethyl acetate (10 mL). Morpholine (2.2 mL, 25 mmol), and N,N-diisopropylethylamine (3.5 mL, 20 mmol) were added and the mixture stirred overnight at room temperature. At 17 h, additional ethyl acetate (150 mL) was added and the combined mixture was washed with water (50 mL) and brine (50 mL); dried (Na2S0 ), filtered and concentrated under vacuum. The residue was used without further purification.
Reference Example 15b: 5-Ατηώο-2^οφηο1ώ- -ν1^ειιζο ίϋτΐ6 This compound was prepared from 2-Μθφ^Ιώ- ^1-5-ώίχο^6ηζοηϊΐπ1β (as prepared in Reference Example 15a above), as prepared in Reference Example 13b.
Reference Example 16 0 Preparation of 3-Fluoro-4-morphoIin-4-yl-phenyIamine Reference Example 16a: 4-(2-Fluoro-4-nitro-phenyl)-morpholine. 3,4-Difluoronitrobenzene (3.7 g, 23.2 mmol) was dissolved in ethyl acetate (10 mL). (2.2 mL, 25 mmol), and N,N-diisopropylethylamine (4 mL, 23 mmol) were added and the mixture stirred overnight at room temperature. At 17 h, additional ethyl acetate (150 mL) was added and the combined mixture was washed with water (50 mL) and brine (50 mL), dried ( a2S04), filtered and concentrated under vacuum. The residue was used without further purification.
Reference Example 16b: 3-Πuoro-4-mo holi -4-yl-phenylamine.
• This compound was prepared from 4-(2-ΠυοΓθ-4-^ΐο-ρηβ^1)^ο ηο1ΐη6, (as prepared in Reference Example 16a above) as prepared in Reference Example 13b.
Reference Example 17 Preparation of 4-(4-Amino-phenyI)-piperazine-l-carboxylic acid tert-butyl ester: Reference Example 17a: 4-(4-Nitro-phenyl)-piperazine-l -carboxylic acid ieri-butyl ester. 4-Fluoronitrobenzene (4.8 g, 34 mmol) was dissolved in ethyl acetate (25 mL). Piperazine-1- carboxylic acid iert-butyl ester (6.7 g, 36 mmol) and N,N-diisopropylethylamine (6.3 mL, 36 mmol) were added and the rnixture was stirred at 65 °C for five days and cooled to room temperature. Ether (100 mL) was added and the combined mixture was washed with water (25 mL) and brine (25 mL), dried (Na2S04), filtered and concentrated under vacuum. The residue was triturated with hexane to yield a bright yellow solid (8 g, 77%).
Reference Example 17b: 4-(4-Amino-phenyl)-piperazine-l-carboxylic acid tert-butyl ester. 4-(4-Arrii-ao-phenyl)-piperazine-l-carboxylic acid ier/-butyl ester was prepared from 4-(4-Nitro-phenyl)-piperazine-l -carboxylic acid ier/-butyl ester, (as prepared in Reference Example 17a) as prepared in Reference Example 13b.
Reference Example 18 Preparation of 3-Morpholin-4-yl-phenylamine Reference Example 18a: 4-(3-Nitro-phenyl)-morpholine. 3-Fluoronitrobenzene (10 g, 71 mmol) was dissolved in acetonitrile (100 mL).
Morpholine (30 mL, 350 mmol) was added and the mixture was reacted 18 h at 150 °C/80psi in a pressure reactor. The reaction was cooled to room temperature, concentrated under vacuum and 5g of the total mixture was purified by column chromatography on silica eluted with CH2CI2. The product (3.6 g) was isolated as a bright yellow oil.
Reference Example 18b: 3-Morpholin-4-yl-phenylamine 3-Morpholin-4-yl-phenylamine was prepared from 4-(3-Nitro-phenyI)-morpholine, (as prepared in Reference Example 18a), as prepared in Reference Example 13b.
Reference Example 19 Preparation of 2-[4-(4-amino-phenyI)-piperazin-l-yI]-ethanol.
Reference Example 19a: 2[4-(4-mtrophenyl)piperazine-l-yl]-ethanol. 2[4-(4-nitrophenyl)piperazine-l-yl]-ethanol is prepared from commercially available 4-fluoronitrobenzene (Aldrich) and commercially available N-(2-hydroxyethyI)piperazine (Aldrich) via the same procedure as described in Reference Example 13a above.
Reference Example 19b: 2-[4-(4-amino-phenyl)-piperazin-l -yI]-ethanol. 2-[4-(4-amino-phenyl)-piperazin-l-yl]-ethanol is prepared by catalytic hydrogenation of 2[4-(4-nitrophenyl)piperazine-l-yI]-ethanol (prepared as in Reference Example 19a) as described in Reference Example 13b Reference Example 20 Preparation of 4-Morpholin-4-yl-phenylamine. 4-(4-Nitrophenyl)morpholine (10.3 g, 49.5 mmol;) (Lancaster Synthesis) was suspended in methanol (130 ml) and 2 M ammonia in methanol (70 mL) and 5 % palladium on carbon (100 mg) was added. The mixture was hydrogenated on a Paar apparatus (50 psi) for 1 h. The reaction was allowed to cool, the catalyst was filtered and the solution was concentrated in vacuo. The crude solid was recrystallized from ethyl acetate / hexane to give as a light purple solid (6.2 g, 70 % yield, mp 132-133 °C).
GC MS (EI, M+) m/z = 178.
Reference Example 21 Preparation of 4-Amino-3-hydroxyphenylmorpholine 4-Nitro-3- hydroxyphenylmorpholine (Maybridge Chemical) (3.34 g, 14.9mmol) was dissolved in 59 ml of ethanol at 30°C. The mixture was stirred at 25°C and treated with tin (II) chloride dihydrate (16.8 grams, 74.5mmol) with stirring. The yellow suspension was heated to reflux over a 30 minute period. TLC showed reaction progress over several hours.. The mixture was refluxed for 18 hours, cooled to room temperature, and concentrated to remove most of the ethanol to give a yellow slurry. The mixture was treated with saturated aqueous sodium bicarbonate until it was basic. The mixture was extracted with ethyl acetate, filtered, and the organic layer was separated. The aqueous layer was extracted twice more · with ethyl acetate. The extracts were combined, dried over magnesium sulfate, filtered, and concentrated to give 1.02 grams of a purple solid. Proton NMR and CI mass spectral analyses were consistent for the desired product (m/z = 195 base peak by positive ion CI and m/z =1 3 base peak by negative ion CI).
WO 02/055014 PCT/SE02/OOO70 Reference Example 22 Preparation of 6-Methoxy-8-(4-methyl-[l,4]diazepan-l-yI)-4-oxo-4H-chromene-2- carboxylic acid . Reference Example 22a: 6-Memoxy-8-(4-methyl-[l ,4]diazepan-l-yI)-4-oxo-4H-chromene-2- carboxylic acid ethyl ester.
Into a 250 mL 3 neck round bottom flask equipped with a reflux condenser, nitrogen inlet and magnetic stirrer is placed 1.5 g (4.59 mmol, 1.0 equiv.) of '8-Bromo-6-methoxy-4- oxo-4H-chromene-2-carboxylic acid ethyl ester (Reference Example 2c), 84 mg (0.092 mmol, 0 0.02 equiv.) of tris dibenzylidineacetone dipalladium, 342 mg (0.55 mmol, 0.12 equiv.) of racemic 2,2'-bis(diphenylphosphino)-l,l '-binapthyl and 2 g of 4 A molecular sieves. To this- suspension is added 150 mL of dry toluene. To the stirred suspension is then added 628 mg, 684 juL, (5.50 mmol, 1.2 equiv.) of 1-methylhomopiperazine, followed by 2.05 g (6.3 mmol, 1.4 equiv.) of cesium carbonate. The mixture is then heated to 80 °C for 3 days. At the- end of 5 this time completion was monitored by LC/MS analysis of an aliquot. When the reaction was determined to be complete it was cooled to room temperature then filtered through a plug of diatomaceous earth with toluene washing to remove solid by products. Purification by flash chromatography, using a gradient of 5 to 20% methanol in methylene chloride as eluent, yielded 1.0 g, (60%) of the desired product.
Mass Spec.: calc. for [Ci9H24N205+H]+ Theor. r /z = 361; Obs. = 361 ■ Reference Example 22b: 6-Memoxy-8-(4-memyl-[l,4]diazepan-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid.
Into a 125 mL erlenmeyer equipped with a magnetic stirrer is placed 319 mg (0.89 mmol, 1.0 equiv.) of 6-Methoxy-8-(4-methyl-[l,4]diazepan-l-yl)-4-oxo-4H-chromene-2- carboxylic acid ethyl ester. This material is dissolved in 30 mL of THF, then 30 mL of methanol are added. To this stirring solution is added 30 mL of a water containing 41 mg (0.97 mmol, 1.1 equiv.) of lithium hydroxide. This mixture is stirred at room temperature for 2 nr. Completion of the reaction is monitored by LC/MS, then 10 mL of 2N HCI is added. This mixture is then concentrated, dried and triturated with ether to give the product as the hydrochloride salt in quantitative yield.
Mass Spec: calc. for [CnH2oN205+H]+ Theor. m/z = 333; Obs. = 333 Reference Example 23 Preparation of 6-Ethoxy-8-(4-methyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carbonyl chloride Reference Example 23 a: 8-Bromo-6-hydroxy-4-oxo-4H-chromene-2-carboxylic acid ethyl ester: The hydroxy compound, 8-Bromo-6-hydroxy-4-oxo-4H-chromene-2-carbox iic acid ethyl ester, is formed as a side product during the synthesis of 8-Bromo-6-methoxy-4-oxo-4H- chromene-2-carboxylic acid ethyl ester. It can be separated from the crude methoxy compound by flash chromatography using a step gradient of 20% ethyl acetate in methylene chloride to the same solvent containing 2% methanol. The hydroxy compound, which elutes last, is concentrated to give the pure compound. Mass Spec: calc. for C|2H Br05+H]+ Theor. m/z = 313, 315; Obs. = 313, 315 Reference Example 23b: 8-Bromo-6-ethoxy-4-oxo-4H-chromene-2-carboxyIic acid ethyl ester: Into a 100 mL 3 neck round bottom flask equipped with a reflux condenser, nitrogen inlet and magnetic stirrer is added 700 mg (2.24 mg, 1.0 equiv.) of 8rBr0mo-6-hydroxy-4-oxo-4H-chromene-2-carboxylic acid ethyl ester (Reference Example 23a). This material is dissolved in 50 mL of toluene, then 689 mg, 586 (4.47 mmol, 2.0 equiv.) of diethyl sulfate and 309 mg (2.24 mmol, 1.0 equiv.) of K2C03 were added. The reaction was then heated to reflux for 24 hr. At the end of this time, monitoring by LC/MS reveals that the reaction is >than 95% complete. The reaction is then cooled, 100 mL of ethyl acetate is added and the organic layer is washed with 0.5N HCI solution, dried over Na2S04, filtered and concentrated. The residues were subjected to flash chromatography, using 40% ethyl acetate in hexane as . eluent. The purified fractions were concentrated to yield 500 mg (65%) of a colorless solid. Mass Spec: calc. for [C^nBrOs+H] Theor. m/z = 341, 343; Obs. = 341, 343 Reference Example 23c: 6-Ethoxy-8 -(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2- carboxylic acid ethyl ester: Into a lOOmL, 3 neck round bottom flask equipped with a reflux condenser, magnetic ■ stirrer and nitrogen inlet is added 350 mg (1.03 mmol, 1.0 equiv.) of 8-Bromo-6-ethoxy-4- oxo-4H-chromene-2-carboxylic acid ethyl ester (Reference Example 23b), 18.9 mg (0.02 mmol, 0.02 equiv.) of tris dibeiizylidineacetone dipalladium, 77 mg (0.123 mmol, 0.12 equiv.) of racemic 2,2'-bis(diphenylphosphino)-l ,1 '-binapthyl and lg of 4 A molecular sieves and 60 0 mL of dry toluene. To the stirred suspension is then added 113 mg, 1255 μΙ_, (1.13 mmol, 1.1 equiv.) of 1 -methylpiperazine, followed by 470 mg (1.44 mmol, 1.4 equiv.) of cesium carbonate. The mixture is then heated to 80 °C for 3 days. At the end of this time completion was monitored by LC/MS analysis of an aliquot. When the reaction was determined to be ' complete it was cooled to room temperature then filtered through a plug of diatomaceous 5 earth, with toluene washing to remove solid by products. Purification by flash chromatography, using a gradient of 5 to 40% methanol in methylene chloride as eluent, yielded 350 mg (75%) of the desired product as a yellow solid. Mass Spec: calc. for [Ci9H24N205+H]+ Theor. m/z = 361; Obs. = 361 Reference Example 23d: 6-Ethoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-0 carboxylic acid: Into a 125 mL Erlenmeyer equipped with a magnetic stirrer is placed 500 mg (1.39 mmol, 1.0 equiv.) of 6-Emoxy-8-(4-memyl-piperazin-l-yl)-4^oxo-4H-chromene-2-carboxylic - acid ethyl ester (Reference Example 23c). This material is dissolved in 30 mL of THF, then 30 mL of methanol are added. To this stirring solution is added 30 mL of a water containing 64.2 mg (1.53 mmol, 1.1 equiv.) of lithium hydroxide. This mixture is stirred at room temperature for 2 hr. Completion of the reaction is monitored by LC/MS, then 10 mL of 2N HC1 is added. This mixture is then concentrated, dried and triturated with ether to give the product as the hydrochloride salt in quantitative yield.
• Mass Spec: calc. for [Ci7H2o 2P5+H]+ Theor. m/z = 333; Obs. = 333 Reference Example 23e: 6-Emoxy-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromene-2- carbonyl chloride: ■ .
Into a 100 mL round bottom flask equipped with a reflux condenser, nitrogen inlet and magnetic stirrer is placed 250 mg (0.68 mmol, 1.0 equiv.) of 6-Ethoxy-8 -(4-methyl-piperazin- l-yl)-4-oxo-4H-chromenei2-carboxylic acid hydrochloride salt (Reference Example 23d) and 20 mL of methylene chloride. To the stirring suspension is then added 129.5 mg, 164 L(1.02 mmol, 1.5 equiv.) of oxalyl chloride followed by addition of one drop of DMF from a 50 microliter syringe to act as catalyst. The mixture is stirred for 2 hours, then concentrated to dryness on a rotary evaporator under a nitrogen atmosphere, followed by drying under high ' vacuum. The completeness of the reaction was ascertained by analysis of an aliquot, which was quenched with a THF solution of memylamine, by LC/MS. The crude material was used as obtained in the subsequent amidation reaction.
Reference Example 24 Preparation of 8-Bromo-6-methoxy-4-(2-trimethylsilanyl-ethoxymethoxy)-quinoline-2- carboxylic acid methyl ester.
Reference Example 24a: 2-(2-Bromo-4-memoxy-phenylamino)-but-2-enedioic acid dimethyl ester.
A solution of 2-bromo-4-methoxy aniline (6.02 g, 29.8 mmol) in 125 mL anhydrous methanol was treated with dimethyl acetylenedicarboxylate (3.70 mL, 30.2 mmol) and the solution was heated at reflux under nitrogen for 8 hours. The reaction mixture was cooled, concentrated, and redissolved in hot methanol. Yellow crystals were obtained by filtration (6.93 g, 68%). A second crop of crystals was obtained from ethanol (0.942 g, 9%). The filtrates were combined and purified by flash chromatography on silica gel using 4: 1 hexanes:ethyl acetate to afford an additional 1.63 g (16%) for a total yield of 93%. Ή NMR (300 MHz, DMSO, d6) δ 9.60 (s, I H, NH), 7.26 (d, 1 H, Jm= 2.7 Hz, ArHj), 6.93 (dd, 1 H, J0= 8.7, Jm= 2.7 Hz, ArHs), 6.87 (d, 1 H, J0= 8.7 Hz, ArH6), 5.34 (s, 1 H, C=CH), 3.76 (s, 3 H, OCH3), 3.68 (s, 3 H, CHCO2CH3), 3.66 (s, 3 H, CNC02CH3); Mass Spec: calc. for [C13Hi4BrN05+H]+ Theor. m/z = 344, 346; Obs. 344, 346.
Reference Example 24a: 8-Bromo-6-methoxy-4-oxo-l ,4-dmydro-quinoline-2-carboxyUc acid methyl ester.
Dow-Therm (175 mL) was heated to '244 °C and the 2-(2-bromo-4-methoxy-phenyIamino)-but-2-enedioic acid dimethyl ester (9.50 g, 27.6 mmol) was added as a solid in portions over 7 minutes while mamtaining a temperature of 230-240 °C. The brown reaction mixture was heated at 240-245 °C for 45 minutes and then cooled to room temperature. A yellow precipitate formed upon cooling. Approximately ] 00 mL of hexanes were added to . the mixture and the solids were isolated by filtration, washed with additional hexanes, and dried under high vacuum to afford the product as a yellow solid (6.73 g, 78%). Ή NMR (300 MHz, DMSO, d«) 5 12.01 (s, 1 H, NH), 7.86 (d, 1 H, Jra= 2.7 Hz, ArHj), 7.52 (s, 1 H, C=CHJ, 7.48 (d, 1 H, Jm= 2.7 Hz, ArH7), 3.93 (s, 6 H, OCH3 and CO2CH3); Mass Spec: calc. for [CnHioBrN04+H]+ Theor. m/z = 312, 314; Obs. 312, 314.
Reference Example 24c: 8-Bromo-6-memoxy-4-(2-1ximemylsilanyl-ethoxymethoxy)- quinoline-2-carboxylic acid methyl ester.
A brown solution of 8-bromo-6-methoxy-4-oxo- 1 ,4-dihydro-quinoline-2- carboxylic acid methyl ester ( 6.73 g, 21.6 mmol) in 100 mL N-methyl pyrohdinone was treated with sodium hydride (60% dispersion in oil, 1.028 g, 25.7 mmol). Gas evolution and wanning were observed. The reaction was stirred for 10 minutes at room temperature under nitrogen. Addition of 2-(trimemylsilyl)ethoxymethyl chloride (5.00 mL, 28.3 mmol) resulted in a slightly cloudy, light brown solution. After 2.5 hours at room temperature, the reaction mixture was poured into 800 mL water and stirred for 15 minutes. The resulting cream colored precipitate was isolated by filtration, washed with water, and dried under high vacuum to afford the product as a cream colored solid' (9.70 g, quantitative yield). Ή NMR (300 MHz, DMSO, d6) δ 7.976 (d, 1 H, Jm= 2.7 Hz, ArH7), 7.79 (s, 1 H, C=CH), 7.53 (d, 1 H, Jm= 2.7 Hz, ArHj), 5.70 (s, 2 H, OCH2O), 3.99 (s, 6 H, OCH3 and CO2CH3), 3.88 (t, 2 H, J= 8.0 Hz, OCH2CH2Si), 0.97 (t, 2 H, J= 8.0 Hz, OCHzC&Si), ), -0.04 (s, 9 H, Si(C H3) 3; Mass Spec: calc. for [Ci8H24BrN05Si+H3+ Theor. m/z = 442, 444; Obs. 442, 444.
Reference Example 25 Preparation of 6- ethoxy-8-(4-methyl-[l,4] diazepan-l-yI)-4-oxo-l,4-di'hydro-quinoIine- 2-carboxylic acid.
Reference Example 25a: 6-Me&oxy-8-(4-memyl-[I,4]diazepan~l-yl)-4-^ . ethoxymemoxy)-qumoline-2-carboxylic acid methyl ester.
To a clear, light brown solution of 2-bromo-6-memoxy-4-(2-trimethylsilanyl- emoxymemoxy)-quinoline-2-carboxylic acid methyl ester (1.01 g, 2.28 mmol), N- methylhomopiperazine (0.32 mL, 2.57 mmol), and 4 A sieves in 30 mL anhydrous toluene was added Pd2 (dba) 2 (43.8 mg, 0.048 mmol) and BINAP (169.8 mg, 0.27 mmol). The resulting wine colored solution was treated with cesium carbonate (1.124 g, 3.45 mmol). The reaction mixture was heated at reflux under nitrogen for 21 hours. The pea green reaction mixture was cooled to room temperature and concentrated. The crude mixture was purified by flash chromatography on silica gel using a gradient of 95:5 to 40:60 methylene chloride: methanol to afford the desired product as a yellow foam (1.004 g, 92%). Ή MR (300 MHz, DMSO, d6) δ 7.67 (s, 1 H, ArHj), 6.94 (d, 1 H, Jm= 2.4 Hz, ArHs), 6.66 (d, 1 H, Jm= 2.4 Hz, ArHj), 5.60 (s, 2 H, OCHjO), 3.94 (s, 3 H, C02CHj), 3.88 (s, 3 H, OCgj), 3.82 (t, 2 H, J= 8.0 ' Hz, OCHzCH^i), 3.75 (bs, .4 H, ArNCHjCH2CH2NCH3 & ArNC&CH2N-CH3), 3.45 (bs, 2 H, 331 (bs, 2 H, ArNCH2CH2C NCH3), 2.83 (s, 3 H, NCH3), 2.28 (bs, 2 H ArNCH2CH2 CH2NC¾), 0.92 (t, 2 H, 8.0 Hz, OCH^&Si), -0.04 (s, 9 H, Si(C H3) 3; Mass Spec: calc. for [C24H37N305Si+H]+ Theor. m/z = 476; Obs. 476.
Reference Example 25b: 6-Memoxy-8-(4-methyl-[l,4]diazepan-l-yl)-4-oxo-l,4-dihydro- quinoline-2-carboxylic acid.
To a light brown solution of 6-methoxy-8-(4-methyl-[l,4]diazepan-l-yl)-4-(2- trimethylsilanyl-emoxymethoxy)-quinoline-2-carboxylic acid methyl ester (1.00 g, 2.10 mmol) in 18 mL 3:1:1 tetrahydrofuran:methanol:water was added lithium hydroxide monohydrate (0.267 g, 6.35 mmol). The reaction mixture was stirred at room temperature for 5 hours, acidified to pH 4 with 1 N HC1, and stirred an additional 20 rninutes. The reaction mixture was concentrated and dried under high vacuum to afford an orange foam. Ή NMR (300 MHz, DMSO, d6) δ 11.06 (s, 1 H, NH), 7.53 (s, 1 H, C=CH), 7.00 (d, 1 H, Jm= 2.4 Hz, ArH ), 6.70 (d, 1 H, Jm= 2.4 Hz, ArH7), 4.05-3.99 (m, 2 H, ArNC&CH2CH2NCH3), 3.87 (s, 3 H, OCH3), 3.68-3.60 (m, 2 H, ArNCHiCH2NCH3), 3.54-3.47 (m, 2 H, ArN(¾CHjNCH3), 3.41-3.26 (m, 2 H, ArNCH2CH2CH2NCH3), 2.82 (d, 3 H, J= 4.8 Hz, NCHj), 2.46-2.41 (m, 1 H ArNCH2CH2CH2NCH3), 2.30-2.25 (ms. 1 H ArNCH2CH2CH2NCH3); Mass Spec: calc. for [Ci7H2iN304+H]+ Theor. m/z = 332; Obs. 332.
Reference Example 26 Preparation of 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo-l54-tlihydro-quinoline-2- carboxylic acid.
This compound was prepared via the same procedure described for preparation of Reference Example 25.
Reference Example 27 . Preparation of 6-Memoxy-8-(4-memyl-[lJ4]diazepan-l-yl)-4-(2-tiimemy silan^ emoxymemoxy)-qumoline-2-carboxylic acid (4-mo^holin-4-yl-phenyl)-arnide.
Reference Example 27a; 8-Bromo-6-methoxy-4-oxo- 1 ,4-dihydro-quinoline-2-carboxyhc acid.
. To a light brown solution of 8-bromo-6-me oxy-4-(2-trimethylsilanyl- -emoxvmemoxy)-qumoline-2-carboxylic acid methyl ester (Reference Example 24c) (4.98 g, 11.3 mmol) in 75 mL 3:1 :1 tetrahydrofuran:methanol:water was added lithium hydroxide monohydrate (1.367 g, 32.6 mmol). The reaction was stirred at room temperature for 5 hours. The reaction mixture was concentrated and then poured into water. The solution was acidified to pH 2 with 1 N HC1 and the resulting solids were isolated by filtration. The solids were then suspended in methanol and filtered to afford the desired product (2.6732 g, 80%). An additional 0.5768 g (17%) of product was obtained from- the methanol filtrates. JH NMR (300 MHz; DMSO, d*, TFA Shake) 5 7.86 (d, 1 H, Jm= 2.7 Hz, ArHs), 7.55 (d, 1 H, Jm= 2.7 Hz, ArH7), 7.32 (s, I H, C=CH), 3.94 (s, 3 H, OCH3); Mass Spec: calc. for [C] 1H8BrN04+H]+ Theor. Jz = 298, 300; Obs. = 298, 300.
Reference Example 27b: 8-Bromo-6~methoxy-4-oxo- 1 ,4-dmydro-quinoline-2-carboxylic acid (4-mo holi -4-yl-phenyl)-amide.
To a yellow suspension of 8-bromo-6-methoxy-4-oxo-l ,4-cl ydro-qukoline-2- carboxylic acid (Reference Example 27a) (3.446g, 11.56 mmol), TBTU (9.039 g, 28.15 mmol), and HOBt ( 3.757 g, 27.8 mmol) in 100 mL dimethylformamide was added 4- moφholmoa iline ( 2;733. g, 15.3 mmol) and diisopropylethyl amine (8.2 mL, 50.2 mmol). The resulting marroon solution was stirred at room temperature under nitrogen for 16 hours during which time the reaction became greenish brown and formed a large amount of precipitate. The reaction mixture was filtered and the solids washed with dimethylformamide, water, and methanol. Drying under high vacuum afforded the desired product as a yellow solid (3.09 g, 58%). Ή NMR (300 MHz, DMSO, d*) δ 12.13 (s, 1 H, NIT), 10.18 (s, 1 H, ' C(O)NH), 7.90 (d, 1 H, Jm= 2.7 Hz, ArH >, 7.68 (d, 2 H, J0= 9.0 Hz, ArHr& Η6·), 7.63 (s, 1 H, C=CH), 7.51 (d, 1 H, Jm= 2.7 Hz, ArH7), 7.00 (d, 2 H, Jo=9.0 Hz, ArHj.&jHs , 3.94 (s, 3 H, OCH3), 3.75 (t, 4 H, J= 4.8 Hz, OCHzCHaN), 3.10 (t, 4 H, J= 4.8 Hz, OCHaCHjjN); Mass ' Spec: calc. for [C2iH2oBrN304+H]+ Theor. m/z = 458, 460; Obs. = 458, 460.
Reference Example 27c: 8-Bromo-6-methoxy-4-(2-trimethylsilanyl-ethoxymethoxy)- quinoline-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide. .
A yellow suspension of 8-bromo-6-memoxy-4-oxo-l,4~dihydro-quinoline-2-carboxylic acid (4-mo holin-4-yl-phenyl)-amide (Reference Example 27b) (3.092 g, 6.75 mmol) in 40 mL N-methylpyrolidinone was treated with sodium hydride (60% dispersion in oil, 0.410 g, 10.24 mmol). Gas evolution and warming were observed and the suspension became light brown and almost clear. The reaction was stirred for 10 minutes at room temperature under nitrogen. Addition of the 2-(trimethylsilyI)ethoxymethyl chloride (1.6 mL, 9.1 mmol) resulted in a slightly cloudy, lighter brown solution. After 4.5 hours at room temperature, the reaction mixture was poured into 300 mL water, stirred for 15 minutes and then stored at 0 °C overnight. The solids were isolated by filtration, suspended in methanol, filtered again, and dried under high vacuum to afford the product as a yellow solid (3.190 g, 80%). Ή NMR (300 MHz, DMSO, d≤) δ 10.18 (s, 1 H, C(O)NH), 7.95 (d, 1 H, Jffi= 2.4 Hz, . ΑτΗτ), 7.83 (s, 1 H, Argj), 7.69 (d, 2 H, J0= 9.0 Hz, Ari^ H6.), 7.51 (d, 1 H, Jm= 2.7 Hz, ArHs), 7.00 (d, 2 H, Jo=9.0 Hz, ArH .& Η5·), 5.69 (s, 2 H, OCH20), 3.95 (s, 3 H, OCH3), 3.85 (t, 2 H, 8.0 Hz, OCH2CH2Si), 3.75 (t, 4 H, J= 4.7 Hz, OCH2CH2N), 3.10 (t, 4 H, J= 4.7 Hz, OCH2CH2N), 0.94 (t, 2 H, J= 8.0 Hz, OCH2CH2S1), -0.04 (s, 9 H, Si(C H3) 3; Mass Spec: calc. for [C27H34Br 305Si+H]+ Theor. m/z = 588, 590; Obs. = 588, 590.
Reference Example 27d: 6-Methoxy-8-(4-methyl:[l ,4]diazepan-l -yl)-4-(2-trimethylsilanyl- ethoxymethoxy)-quinoline-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide.
To' a yellow-green suspension of 8-bromo-6-memoxy-4-(2-trimethylsilanyl- ethoxymethoxy)-quinoline-2-carboxylic acid (Reference Example 27c) (4-morpholin-4-yl- phenyl)-amide (1.155 g, 1.96 mmol), N-methyl homopiperazine (0.39 mL, 3.14 mmol), and 4 A sieves in 30 mL anhydrous toluene was added Pd2 (dba) 2 (90.0 mg, 0.098 mmol) and BINAP (0.358 g, 0.58 mmol). The resulting reddish brown mixture became lighter in color upon treatment with cesium carbonate (2.544 g, 7.81 mmol). The reaction mixture was heated at reflux under nitrogen for 17 hours. The clear brown solution was cooled to room temperature, concentrated, and then purified by flash chromatography on silica gel using a slow gradient of 95:5 to 50:50 methylene chloride methanol to afford the desired product ' (0.989 g, 81%). Ή NMR (300 MHz, DMSO, d6) 5 9.88 (s, 1 H, NH), 7.73 (s, 1 H, Argj), ' 7.68 (d, 2 H, J0= 8.9 Hz, ArHr& Η6·), 7.00 (d, 2 H, J0= 8.9 Hz, ArHj.&iis , 6.94 (d, 1 H, Jm= 2.7 Hz, AxHs), 6.66 (d, 1 H, Jm= 2.7 Hz, ArH7), 5.62 (s, 2 H, OC¾0), 3.87 (s, 3 H, OCH3), 3.80(t, 2 H, J= 8.0 Hz, OCHzCH^i), 3,73 (t, 4 H, J= 4.7 Hz, OCHzCHzN), 3.63 (t, 2 H, J= 5.9 Hz, ArNCH2CH2CH2NGH3), 3.33 (bs, 2 H, ArNCEbCHaNCHs), 3.09 (t, 4 H, J= 4.7 Hz, OCH2GH2N), 2.97 (bs, .2 H, ArNCH2CH2NCH3)5 2.69 (bs, 2 H, ArNCH2CH2CH2NCH3), 2.35 (s, 3 H, NCHj), 2.09 (bs, 2 H ArNCH2CHjCH2NCH3), 0.94 (t, 2 H, J= 8.0 Hz, OCHzCHzSi), -0.03 (s, 9 H, Si(C Hj) 3; Mass Spec: calc. for [C33H47N50sSi+H]+ Theor. m/z = 622; Obs. = 622.
Reference Example 28 Preparation of 8-Bromo-4-dimethylamino-6-methoxy-quinoIine-2-carboxyIic acid (4- morpholin-4-yI-phenyI)-amide.
Reference Example 28a: 8-Bromo-4-cmoro-6-methoxy-qumoline-2-carboxylic acid (4- mo boli -4-yl-phenyl)-amide .
A suspension of 8-bromo-6-methoxy-4-oxo- 1 ,4-dihydro-qumoline-2-carboxylic acid (Reference Example 27b) (1.75 mmol) in 20 mL methylene chloride was treated with oxalyl chloride (1.5 mL, 17.2 mmol) and catalytic dimethylformamide (3 drops). The reaction mixture bubbled vigorously and became clearer. The reaction was heated at reflux for 2 hours, cooled to room temperature, and concentrated to a pale yellow solid (kept under nitrogen). ' To a yellow solution of the acid chloride in 20 mL methylene chloride was added 4- morphblinoaniline (0.347 g, 1.94 mmol) and diisopropylethyl amine (1.0 mL, 6.1 mmol). The solution became orange and gas evolution was observed. Within 30 minutes, solids began to precipitate from the solution. The reaction was stirred at room temperature for 1 hour. The solids were isolated by filtration and dried under high vacuum to afford the desired product (0.406 g, 49%). ]H NMR (300 MHz, DMSO, d≤) δ 10.15 (s, 1 H, C(O)NH), 8.33 (s, 1Ή, Argj), 8.10 (d, 1 H, Jm= 2.7 Hz, ArHyJ, 7.70 (d, 2 H, J0= 9.0 Hz, ArH2-& Η6·), 7.56 (d,- 1 H, Jm= 2.7 Hz, ArHs), 7.01 (d, 2 H, Jo=9.0 Hz, ΑτΗ3·& Η5·),Α06 (s, 3 H, OCH3), 3.75 (t, 4 H, J= 4.8 Hz, OCH2CH2N), 3.11 (t, 4 H, J= 4.8 Hz, OCHzCHjN); Mass Spec: calc. for .
. [C2|Hi9BrClN303+H]+ Theor. m z = 476, 478; Obs. = 476, 478.
Reference Example 28b: 8-Bromo-4-dimemylainino-6-methoxy-quinoline-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide.
A solution of 8-bromo-4-chloro-6-methoxy-quinoline-2-carboxylic acid (4-morpholin- ' 4-yl-phenyI)-amide (Reference Example 28a) (0.1512 g, 0.317 mmol) in 100 mL 2.0 M dimethyl amine in tetrahydrofuran was heated at 100 °C in a Parr bomb. The initial pressure was 75-80 psi and then remained at approximately 60 psi. After 18 hours, the reaction was cooled to room temperature, concentrated and dried to afford the crude product as a brown solid. Purification on silica gel using a gradient of 100:0 to 95:5 methylene chloridemethanol afforded the clean product (0.142 g, 92%). *H NMR (300 MHz, DMSO, de) δ 10.20 (s, 1 H, C(O)NH), 7.90 (d, 1 H, Jffi= 2.7 Hz, ArH≤),-7.69 (d, 2 H, J0= 9.0 Hz, ArHr& Η6·), 7.60 (s, 1 H, ArHj), 7.41 .(d, 1 H, Jm= 2.7 Hz, ArH7), 7.01 (d, 2 H, Jo=9.0 Hz, ArHj-& Ms'), 3.96 (s, 3 H, OCH3), 3.75 (t, 4 H, J= 4.8 Hz, OCHzCHzN), 3.10 (t, 4 H, J= 4.8 Hz, OCH2CH2N), 3.08 (s, 6 H, N(CH3) 2); Mass Spec: calc. for [C2iHI9BrClN303+H]+ Theor. mlz = 485, 487; Obs. 485, 487 Reference Example 29 Preparation of 6-Fluoro-4-methoxy-8-(4-methyl-piperazin-l-yl)-quinoline-2-carboxylic acid Reference Example 29a: 8-Bromo-6-flu0ro-4-memoxy-qumoline-2-carboxylic acid methyl ester Into a 150 mL 3 neck round bottom flask equipped with a reflux condenser, magnetic stirrer and nitrogen inlet is placed 2.0 g (6.76 mmol, 1.0 equiv.) of 8-Bromo-6-fluoro-4-oxo- l,4-ddhydro-quinoline-2-carboxylic acid methyl ester. This material is then dissolved hi 50 mL of NMP. Then 300 mg (7.44 mmol, 1.1 equiv.) of a 60% dispersion of sodium hydride in oil is cautiously added portion-wise to the solution at room temperature. A yellow color then develops, indicating that formation of the anion has occurred, with hydrogen evolution. Stirring of the anion solution is continued for one hour, then 1.14 g, 500 LL (8.04 mmol, 1.2 equiv.) of iodomethane is added via syringe. The mixture is allowed to react for two hours additional, then is cautiously quenched with 20 mL of water. The solids, which precipitate upon dilution in 1L of water, are collected by filtration, then washed with water to give the pure O methylated material as 2.1 g (98%) of a colorless solid.
Mass Spec: calc. for [Ci2H9BrFN03+H]+ Theor. m/z = 314, 316; Obs. = 314, 316 Alternatively, into a 100 mL 3 neck round bottom flask equipped with a reflux condenser, nitrogen inlet and magnetic stirrer is placed 350 mg (1.17 mmol, 1.0 equiv.) of 8-Bromo-6-fluoro-4-oxo- 1 ,4-o^ydro-quinolme-2-carboxylic acid methyl ester and 242 mg (1.75 mmol, 1.5 equiv.) of K2C03. This material is suspended in 20 mL of DMSO then heated to 70 °C for 1 hr. The anion formation of the anion is apparent when the mixture · becomes cloudy. The mixture is allowed to cool to 35 °C then 331 mg, 145 ]iL (2.33 mmol, 2,0 equiv.) of methyl iodide are added and stirring is continued for 2 hr. At the end of this time it is determined if the reaction is complete by LC/MS. Upon completion the mixture is poured into 200 mL of water and the solids whic form are collected by filtration and washed with water to give 340 mg (93%) of the O-methylated product after drying.
Reference Example 29b: 6-Fluoro-4-methoxy-8-(4-methyl-piperazin- 1 -yI)-quinoline-2- 5 carboxylic acid methyl ester Into a 250mL, 3 neck round bottom flask equipped with a reflux condenser, magnetic stirrer and nitrogen inlet is added 2.1 g (6.68 mmol, 1.0 equiv.) of 8-Bromo-6-fluoro-4^ methoxy-quinoline-2 -carboxylic acid methyl ester (Reference Example 29a) (122 mg, 0.134 mmol,- 0.02 equiv.) of tris o benzylidineacetone dipalladium, 499 mg (0.802 mmol, 0.12 equiv.) of racemic 2,2'-bis(diphenylphosphino)-l,l '-binapthyl and lg of 4 A molecular sieves and 80 mL of dry toluene. To the stirred suspension is then added 736 mg, 815 uL, (7.35 mmol, 1.1 equiv.) of 1 -methylpiperazine, followed by 3.05 g (9.35 mmol, 1.4 equiv.) of cesium carbonate. The mixture is then heated to 80 °C for 36 hr. At the end of this time completion was monitored by LC MS analysis of an aliquot. When the reaction was determined to be complete it was cooled to room temperature then filtered through a plug of celite, with toluene Washing to remove solid by products. Purification by flash chromatography using a gradient of 5 to 20% methanol in methylene chloride as eluent yielded 2.0 g, (90%) of the desired product. Mass Spec: calc. for [Ci7H2oFN303+H]+ theor. ■ m/z = 334; Obs. = 334 Reference Example 29c: 6-Fluoro-4-memoxy-8-(4-memyl-piperazin-l-yl)-quinoline-2- carboxylic acid Into a 125 mL erlenmeyer flask containing 30 mL of THF and 30 mL of methanol is placed 2.1 g (6.3 mmol) of 6-Fluoro-4-methoxy-8-(4-methyl-piperazin-l-yl)-quinoline-2- carboxylic acid methyl ester (Reference Example 29b). To this solution is added with stirring 25 30 mL of water in which is dissolved 291 mg (6.9 mmol, 1.1 equiv.) of lithium hydroxide monohydrate. This solution is allowed to react for 1 hr then is'quenched with 10 mL of 2N HC1 solution. The solution is then filtered and the solids- washed with 10 mL of 0.5 N HC1 solution. The combined filtrates are then concentrated to give 2.15 g, (95%) of the solid • yellow product as the hydrochloride salt. Mass Spec: calc. for [Ci6H]8FN30 +H]+ Theor. m z 0 = 320; Obs. = 320 Example 1 8-(4-metoyl- 1 -piperazmyl)-N-[4-^ carboxamide. 8-(4-methyl- 1 -piperazinyl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) (400 mg, 1.23 mmol) was suspended in anhydrous N,N- dimethylformamide (20 ml) and triemylamine (0.69 ml, 4.92 mmol) was added to give a clear solution. The following were added in order: l-hydroxybenzotriazole (HOBt (205 mg, mol)), · 0-(lH-Benzotriazol-l-yl)-N,N,N',N'-pentamethylene-uronium tetrafluoroborate (TBTU (435 mg, 3.1 mmol)) then 4-(dimemylamino)pyridine (25 mg). After stirring for 5 min at room temperature, 4-(4-morpholmyl)aniline (Reference Example 21) (220 mg, mmol). The reaction stirred overnight at room temperature. The solution was concentrated in vacuo, the remains were partitioned between chloroform / saturated sodium bicarbonate, extracted (x3) with chloroform, dried (MgS0 ) and concentrated in vacuo to give the crude product.
Chromatography on silica (230 - 400 mesh ASTM) and eluting ethyl acetate followed by 2.5-5% methanol / chloroform gave 190 mg ( % yield) of 8-(4-methyl-l-piperazinyl)-N-[4-(4-morpholmyl)phenyl]-4-oxo-4H-benzochromene-2-carboxamide as a yellow solid (mp 217-218° decomposition and melt 244-247C). LC/MS .(M+l) m/z = 449.
Example 2 2-{ 1 -[4-(2-Methoxy-phenyl)-piperazih- 1 -yI]-methanoyI}-8-(4-methyl-piperazin-l-yI)- chromen-4-one. · This compound was prepared from 8-(4-Methyl-piperazin-l -yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available l-(2- Methoxy-phenyl)-piperazine (Aldrich) via the same procedure used in example 1, yielding a yellow solid. MS (M+H) mlz = 463. ' ".
Example 3 2-{ 1 -[4-(l -Acetyl -2,3-dihydro- lH-indol-6-yl)-piperazin-i -yl]-methanoyl} -8-(4-methyl-piperazin- 1 -yl)-chromen-4-one.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and l-(6-Piperazin-l-yl-2,3-dihydro-indol-l-yI)-ethanone (Reference Example 8) as prepared i Example 1, yielding a yellow solid. MS (M+H) m/z = 516.
Example 4 2-Chloro-5-(4- { 1 -[8-(4-methyl-piperazin- 1 -yI)-4-oxo-4H-chromen-2-yl]-methanoyl }- piperazin-1 -yl)-benzonitrile.
This compound was prepared from 8-(4-Methyl -piperazin-1 -yI)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and 2-chloro-5-piperazin-l-yl benzonitrile (Reference Example 9) as prepared in Example 1, yielding a yellow solid. MS (M+H) m/z = 493.
Example 5 2-{l-[4-(4-Memoxy-phenyl)-piperazm-l-yl]-methanoyl}-8-(4-methyI-piperazin-l-yl)-cnromen-4-one.
This compound was prepared from 8-(4-Memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and commercially available (Aldrich) l-(4-Methoxy-phenyl)-pipefazine as prepared in example 1, yielding a yellow solid. MS (M+H) m/z = 463. ' Example 6 8-(4-Methyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid (5-furan-2-yI-lH-pyrazol- 3-yl)-amide. .
This compound was prepared from 8-(4-Methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-• carboxylic acid hydrochloride (Reference Example 1 ) and commercially available 5rfuran-2- yl-lH-pyrazol-3-ylarnine (Maybridge) as prepared in example 1, yielding a yellow solid. MS (M+H) m/z = 420..
Example 7 ' 8-(4-Memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-irnidazol-l-yl-phenyl)- amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yI)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 4- iimdazol-l-yl-phenylarnine (Aldrich) as prepared in Example 1, yielding a yellow solid. MS ' (M+H)m/z = 430.
Example 8 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chrdmene-2-carboxylic acid (4-[ 1 ,2,3]tbiadiazol-5-yl- phenyl)-amide.
This compound was. prepared from 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and 4-[l,2,3]thiadiazol-5-yl- phenylamine (Reference Example 10) as prepared in Example 1* yielding a yellow solid. MS (M+H)m/z = 448.
Example 9 8-(4-Methyl-piperazin- L-yl)-4-oxo-4H-chromene-2-carboxylic acid 4-[ 1 ,2,3]thiadiazol-5-yl-benzylamide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and commercially available (Maybridge) 4-[l)2,3]thiadiazol-5-yl-benzylamine as prepared in Example 1, yielding a yellow solid. MS (M+H) m/z = 462.
Example 10 S-(4-Methyl-pipei^m-l-yI)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-acetyl-piperazin-l- yl)-phenyl] -amide.
This compound was prepared from 8-(4-M ethyl -piperazin-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and l-[4-(4-amino-phenyl)-piperazin- l-yl]-ethanone (Reference Example 11) as prepared in Example 1, yielding a yellow solid. MS (M+H) m/z = 499.
Example 11 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-cafboxylic acid [4-(4-methanesulfonyl-' piperazin-l-yl)-phenyl] -amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and 4-(4-memanesuifonyl-piperazin- 1 -yl)-phenylamine (Reference Example 12) as prepared in Example 1 , yielding a yellow solid MS (M+H) m/z = 526.
Example 12 8-(4-Methyl-piperazin- 1 ^yl)-4-oxo-4H-chromene-2-carboxylic acid (2-methoxy-4-morpholin- 4-yl-phenyl)-arnide. 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) (0.10 g, 0.35mmol), HOBt (0.10 g, 0.7mmol), TBTU (0.225 g, . 0.7nmiol), -(dimemylamino) pyridine (0.01 g, catalytic amount), triemylamine (0.15 mL, . 1.04mmol), and commercially available 2-memoxy-4-morpholm-4-yl-phenylamine (SALOR) (0.08 g, 0.38mmol) were dissolved in dimethylformamide (2.5 mL) and stirred at room temperature overnight. Ethyl acetate (150 mL) was added and the resulting mixture was washed with water (3 x 50 mL), dried (Na2SC>4), filtered, concentrated under vacuum and triturated with ether to yield a yellow solid (85 mg, 54%). LCMS: m/z = 480.3 Example 13 8-(4-Methyl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylic acid (3-chloro-4-morpholin-4-yl-phenyl)-amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yI)-4-oxd-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 3-chloro- (Maybridge) as prepared in Example 12, yielding a yellow solid. (110 mg = 73%), LCMS - m/z = 483.5 Example 14 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-thiomorpholin-4-yl- phenyl)-amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yI)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride. (Reference Example 1) and 4-thiomorpholin-4-yI- phenylamine (Reference Example 13) as prepared in Example 12, yielding a yellow solid. (55 mg = 38%), LC S - m/z = 465.5 Example 15 , 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (2,5-dietiioxy-4- morpholin-4-yl-phenyl)-amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 2,5- diemoxy-4-morpholm-4-yl-phenylamine (Aldrich) as prepared in Example 12, yielding a yellow solid. (80 mg = 50%), LCMS - m/z = 537.6 Example 16 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-cyanomethyl-phenyl)- amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-ox6-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available, (4- amino-phenyl)-acetonitrile (Aldrich) as prepared in Example 12, yielding a yellow solid. (65 mg = 54%), LCMS - m/z = 403.5 Example 17 8-(4- emyl-piperazin-l-yl)-4-qxo-4H-chromene-2-carboxylic acid (lH-indol-5-yl)-amide.
This compound was prepared from 8-(4-Memyl-piperazm-l-yl)-4-oxo-4H-'chromener 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 1H- indol-5-ylamine (Aldrich) as prepared in Example 12, yielding a yellow solid. (35 mg = 15 29%), LCMS - m/z = 401.6 Example 18 8-(4-Methyl-piperazin-l -yl)-4-oxo-4H-chromene-'2-carboxylic acid [4-(l -Μθ ηο1ώ-4-ν1- methanoyl)-phenyl] -amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and l-(4-amino-phenyl)-l- mo holm-4-yl-methanone (Reference Example 14) as prepared in Example 12, yielding a yellow solid. (21 mg = 15%), LCMS - m/z = 477.6 Example 19 8-(4-Methyl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(2,6-dimethyl- mo oli -4- l)-phenyl]-amide. - This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 4-(2,6- dimethyl-moφholin-4-yl)-phenylaπ ine (Maybridge) as prepared in Example 12, yielding a yellow solid. (60 mg = 42%), LCMS - m/z = 477.6 Example 20 8-(4-Methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-fluoro-phenoxy)-phenyl]-amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 4-(4- fluoro-phenoxy)-phenylamine (Maybridge) as prepared in Example 12, yielding a yellow solid. (110 mg = 77%), LCMS - m/z = 475.6 Example 21 8-(4-Me&yl-piperazm-l-yl)-2-(6-morpto 8-(4-Methyl-piperazin-l-yl)-4-ox"o-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) (0.532 g, 1.85mmbl) was placed in a 25 mL 3-neck flask under nitrogen and treated with PPA (6 g). The mixture was then treated with the prepared intermediate 4-ammo-3-hydroxyphenylmorpholine (0.43 g of -85% pure, ~2mmoI). The mixture was stirred and heated- in an oil bath to 205 °C for 3 hours to give a dark liquid. The mixture was cooled to room temperature and treated with 10 mL of water to give a dark solution. The solution was slowly neutralized with IN aqueous sodium hydroxide to pH~7 as a solid formed. The solid was collected, washed several times with water, air dried, and vacuum dried at room temperature to give 0.65 g of a black solid. TLC (10%MeOH in CHCI3 on S1O2) showed 2 major components at Rf~0.5 and several lower Rf minor' components. The solid was triturated with saturated aqueous sodium bicarbonate at room temperature. It was filtered off, washed several times with water, and air dried to give 0.65 g of a dark gray solid. TLC showed the same components seen previously. Mass spectral analysis showed m/e = 447 by positive ion CI and m/e = 446 by negative ion CI. The solid was dissolved in 2% methanol in chloroform and it was chromatographed on a Megabond Elute silica gel column (10 g of Si02) using 2% methanol in chloroform. The slightly faster Rf yellow component was concentrated to give 0.0188 g of a yellow solid. CI mass spectral analysis showed m/e = 447 as the base peak by positive ion CI. The solid was recrystallized in methanol to give 0.0178 g of a yellow solid with a melting point of 158.1-158.8°C. Proton NMR (CDC13) and CI mass spectral analyses were consistent for the desired product (m/z = 447 base peak by positive ion CI and m/z = 446 base peak by negative ion CI).
Example 22 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (2-hydroxy-4-moφholin- 4-yl-phenyl)-amide. 8-(4-memyl-piperazm-l-yi)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) (0.3768 g, 1.16 mmol) was placed in a 100 mL 3-neck flask under nitrogen and it was dissolved in 20 mL of DMF. The solution was treated with Iriemylamine (0.49mL, 3.5mmol) followed by HOBT hydrate (0.36g, 2.3mmol) followed by TBTU (0.74 g, 2.3mmol) and then followed by DMAP (0.020 g). The mixture was stirred for 10 minutes and then it was treated with 4-ammo-3-hydroxyphenylmorpholine (Reference example 21) (0.228 g, 1.17 mmol). The mixture was stirred for 15 minutes and then it was treated with triethylamine (0.17 mL, 1.2 mmol). The mixture was stirred at room temperature for 42 hours and then it was added to a solution of 50 mL of saturated aqueous sodium bicarbonate and 50 mL of water. The mixture was extracted 4 times with ethyl acetate, dried over magnesium sulfate, filtered, and concentrated to give 0.834 gram of a purple oil. The oil was dissolved in 2 percent methanol in chloroform and it was placed on a silica gel column (5.5 cm diameter by 10.5 cm long) and eluted with 2 percent methanol in chloroform followed by 5 percent methanol in chloroform. The yellow fraction was concentrated to give 0.2031 gram of an orange-yellow solid. The solid was dissolved in methanol, filtered through a medium sintered glass funnel, and concentrated to a few ml volume as a solid formed. The solid was filtered off, washed with methanol, and air dried to give 0.1613 gram of a tan solid with MP of 248.4 - 249.6°C. Proton COSY MR and CI mass spectral analyses were consistent for the desired product (m/z = 465 by positive ion CI and m/z = 463 by negative ion CI).
Example 23 8-(4-Me yl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylic acid (5-e oxy-benzothiazol-2- yl)-amide.
. This compound ' was prepared from 8-(4-Memyl-pipera2in-l-yI)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 5-ethoxy- benzot azol-2-ylamine (SALOR) as prepared in Example 12, yielding a yellow solid. (55 mg = 39%), LCMS - m/z = 465.3 Example 24 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-bromo-phenyl)-amide.
This compound was prepared from 8-(4-Memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and commercially available 4-bromo-phenylamine (Aldrich) as prepared in Example 12, yielding a yellow solid. (1.0 g = 75%), LCMS - m/z = 442.4 Example 25 8-(4-Memylpiperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid memyl-(4-morpholin-4-yl- phenyl)amide. 8-(4-Memyl-piperazm-l-yI)-4^oxo-4H-chromene-2-carboxylic acid (4-morpholin-4- yl-phenyl)-amide (Example 1) (0.1046 g, 0.2332 mmol) was placed in a 10 mL single neck round flask under nitrogen. The solid was dissolved in 2.8 mL of anhydrous DMF. The yellow solution was stirred at room temperature and treated with one portion of sodium hydride (0.011 g of 95%, 0.44 mmol). The mixture evolved gas and became- a red solution. It was stirred under nitrogen for 20 minutes and then it was treated with iodomethane (0.015 mL, 0.033 g, 0.233 mmol). The mixture was sealed and stirred at room temperature for 18 hours.
The reaction mixture was concentrated to remove most of the DMF (35 C bath @ 0.5 mm) to give a dark semisolid. It was treated with a few drops of water followed by 10 mL- of ethyl acetate. The mixture was dried over magnesium sulfate, filtered, and concentrated to give 0.0564 gram of a yellow glass. The glass was triturated with diethyl ether, filtered off, and dried under high vacuum to give 0.0302 g of a tan solid with MP of 245.0 - 246.8 C. Proton MR and CI mass spectral analyses were consistent for the desired product (m/z = 463 by positive ion CI).
Example 26 8-(4-Me1jiyl-piperazm-l-yl)-4-oxo-4H-crjxomene-2-carboxylic acid (3-morpholin-4-yI- phenyl)-amide.
This compound was prepared from 8-(4-Memyl-piperazin-l-yl)-4-oxo-4H-chromene- 2-carboxylic acid hydrochloride (Reference Example 1) and 3-morpholin-4-yl-phenylamine (Reference Example 18) as prepared in Example 12, yielding a yellow solid. (120 mg = 86%), LCMS - m/z = 449.5 Example 27 8-(4-Methyl-piperazm- 1 -yI)-4-oxo-4H-chromene-2-carboxylic acid (3-cyano-4-moroholin-4- yl-phenyl)-amide.
This compound was prepared from'8-.(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-• 2-carboxylic acid hydrochloride (Reference Example 1) and 5-amino-2-morpholin-4-yl- benzonitrile (Reference Example 15) as prepared in Example 12, yielding a yellow solid. (120 mg = 82%), LCMS - m/z = 474.5 Example 28 8-(4-Methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-carboxylic acid yl-phenyl)-amide.
This compound was prepared from 8-(4-Methyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 1) and 3-ΑαοΓθ-4-ιηοφ1αοϋη-4-γ1-phenylamine (Reference Example 16) as prepared in example 12, yielding a yellow solid. (120 mg = 83%), LCMS - m/z = 467.6 Example 29 4-[4-( { 1 -[8 -(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromen-2-yl] -methanoyl } -amino)-phenyl] - piperazine-l-carboxylic acid tert-bntyl ester.
This compound was prepared from 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chrpmene- 2-carboxylic acid hydrochloride (Reference Example 1) and 4-(4-amino-phenyl)-piperazine- 1-carboxylic acid ieri-butyl ester (Reference Example 17) as prepared in example 12,' yielding a yellow solid. (260 mg = 53%), LCMS - m/z = 548.6 Example 30 8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin-l.-yl-phenyl)-amide. 4-[4-({ l-[8-(4-Memyl-piperazm phenyl]-piperazine-l-carboxylic acid tert-bntyl ester (Example 29) (160 mg, 0.3 mmol) was dissolved ethyl acetate (20 mL) and cooled to 0°C. HCl gas was bubbled in slowly for 2 minutes. A solid began to precipitate. Methanol (3-4 mL) was added to dissolve this solid and HCl gas was bubbled in for another 2 minutes. The mixture was concentrated under reduced pressure and triturated with ether and dried under vacuum to yield a tan solid (100 mg, 76%). LCMS/ m/z = 448.6 Example 31 6-Methoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-morphoIin- 4-yl-phenyI)-amide: · 6-Memoxy-8-(4-Memyl-piperazm-l-yl)-4-oxc^H-cbxomene-2-carboxylic acid hydrochloride (Reference Example 2) (3.0g, 8.5 mmol), TBTU (5.5g, 17 mmol), 1- hydroxybenztriazole (2.6g, 17 mmol), 4-dimemylammopyridine (0.05g, catalytic) and commercially available (1.66g, 9.3 mmol) were dissolved in dimethylfonnamide (100 mL). Triethylamine ( 3.5 mL, 25 mmpl was added and this mixture stirred at room temperature for 17 hours. The reaction mixture was concentrated under vacuum and the residue was partitioned between chloroform (400 mL) and saturated aqueous sodium bicarbonate' (50 mL). The organic layer was separated, dried (Na2SC>4), vacuum-filtered and concentrated under vacuum. The residue was purified by chromatography on silica eluted with 2- 5% methanol in chloroform and then triturated with ether to yield a yellow powder. (1.6 g = 39%) LCMS - m/z = 479.5 mp = 234-236 °C.
Example 32 6-Methoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-methanesulfonyl-piperazin- 1 -yl)-phenyl]-amide.
This compound was prepared from 6-Methoxy-8-(4-methyl-piperaziii-l -yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 2) and 4-(4-methanesulfonyl- piperazin-l-yl)-phenylamine (Reference Example 12) as prepared in example 1, yielding a yellow solid. GC/MS (EI, M+) m/z = 556 Example 33 6-Methoxy-8-(4-Methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-carboxylic acid (3-chloro-4- morpholin-4-yl-phenyI)-amide. , This compound was prepared from 6-Methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 2) and commercially available 3-chloro-4-morpholm-4-yl-phenylamine (Maybridge) as prepared in Example 12, yielding a yellow solid. (45mg = 31%) LCMS - m/z = 513.5 Example 34 6-Memoxy-8-(4-memyl-pip'erazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid (3-fhioro-4-morpholin-4-yl-phenyl)-amide. ' This compound was prepared from 6-methoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 2) and 3-fiuoro-4-morpholin-4-yl-phenyIamine (Reference Example 16) as prepared in Example 12, yielding a yellow solid. (55mg = 61%), LCMS - m z = 497.5 Example 35 6-Methoxy-8-(4-methyl-piperazb-l-yl)-4-oxo-4H-chromene-2-carbo acid (2-methoxy-4 morpholm-4-yl-plienyl)-amide.
This compound was prepared from 6-methoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 2) and commercially available 2-methoxy-4-mo holin-4-yl-phenylamine (SALOR) as prepared in Example 12, yielding a yellow solid (55mg = 38%), LCMS - m/z = 510.5 Example 36 . 6-Methoxy-8-(4-methyl-piperazm-l-yl)-4-oxo-4HTchromene-2-carboxylic acid (4-thiomorpholin-4-yI-phenyl)-amide.
This compound was prepared from 6-methoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 2) and phenylamine (Reference Example 13) as prepared in Example 12, yielding a yellow solid. (99mg = 71%), LCMS - m/z = 495.5 Example 37 6-Methoxy-8-(4-methyl-pipera2in- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(2,6- οη^β^1^ο ηο1ώ-4^1)^β^1]-3ΐτη^β.
This compound was prepared from 6-methoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 2) and commercially available 4-(2,6-dimethyl-mo hoIm-4-yl)-phenylamine (Maybridge) as prepared in Example 12, yielding a yellow solid. (70mg = 49%), LCMS - m/z = 507.5 Example 38 6-Memoxy-8-(4-memyl-piperazm-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (3-πιθφ1ιο1.η-4-yl-phenyl)-amide.
This compound was prepared from 6-memoxy-8-(4-Memyl-piperazm-l-yI)-4~oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 2) and 3^οφ ο.ϊη-4-^-phenylamine (Reference Example 18) as prepared in Example 12, yielding a yellow solid. (80mg = 60%), LCMS - m/z = 479.5 Example 39 6-Memoxy-8-(4-methyl-piperazinTl -yI)-4-oxo-4H-chrbmene-2-carboxylic acid {4-[4-(2- hydroxy-ethyl)-piperazin- 1 -yl]-phenyl} -amide.
This compound was prepared from 6-methoxy-8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H- . chromene-2-carboxylic acid hydrochloride (Reference Example 2) and 2-[4-(4-amino- phenyl)-piperazin-l-yl]-ethanol (Reference Example 19) as prepared in Example 12, yielding a yellow solid. (80mg = 60%). mp = 21 1.5-212.2 (dec), MS - base peak at m/z =492 by positive ion and m/z =490 by negative ion CI Example 40 6-Methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxyUc acid [4-(l-morpholm-4-yl-memanoyl)-phenyl]-amide.
. This compound was prepared from 6-memoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 2) and 1 -(4-amino-phenyl)- 1 -morpholin-4-yl-methanone (Reference Example 14) as prepared in Example 12, yielding a yellow solid. (170mg = 80%), LCMS - m/z = 507.5 Example 41 6-Memoxy-8-(4-memyl-piperazm-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (3-cyano-4- · mo oli -4-yl- -len l)-amide. ■ .
This compound was prepared from 6-methoxy-8-(4-Methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 2) and 5-amino-2-morpholin- 4-yl-benzonitrile (Reference Example 15) as prepared in Example 12, yielding a yellow solid. (120mg = 57%), LCMS - m/z = 504.5 Example 42 4-[4-( { 1 -[6-Methpxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromen-2-yl]-methanoyl }- amino)-phenyl] -piperazine-1 -carboxylic acid teri-butyl ester.
The 6-methoxy-8-(4-memyi-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 2) (1.04 g, 2.93 mmol) was placed in a 250 ml 3-neck flask under nitrogen and it was dissolved in 50 ml of DMF. The solution was treated with triemylarnine (1.22 mL, 8.79 mmol) followed by HOBT hydrate (0.90 g, 5.9 mmol) followed by TBTU (1.88 g, 5.9 mmol) and then followed by DMAP (0.056 g, 0.46 mmol). The mixture was stirred for 10 minutes and. then it was treated with 4-(4-Amino-phenyl)-piperazine-l-' carboxylic acid ieri-butyl ester (Reference Example 17) (0.81 g, 2.9 mmol). The mixture was stirred for 15 minutes and then it was treated with triemylarnine (0.41 mL, 2.9 mmol). The mixture was stirred at room temperature for 18 hours and then it was concentrated (1 mm Hg pressure, 45 C bath) to give a dark liquid. The concentrate was treated with 80 mL of saturated aqueous sodium bicarbonate and extracted with ethyl acetate fonning a suspended yellow solid in the organic layer. The solid was filtered off, washed with diethyl, ether, washed with water, and vacuum dried (0.1mm Hg pressure @ 25C) to give 0.36 gram of a yellow solid, .P. = 232.3-232.8 C.
Proton NMR and CI mass spectral analyses were consistent for the desired product (m/e = 578 by positive ion CI and m/e = 576 by negative ion CI).
The aqueous layer was extracted twice with ethyl acetate, dried over magnesium sulfate, filtered, and concentrated to give 1.35 gram of a dark semisolid It was triturated with diethyl ether and allowed to stand at room temperature as a solid formed. The solid was filtered off, washed with diethyl ether, and vacuum dried at room temperature to give 0.4816 gram of a-yellow solid. CI mass spectral analyses was consistent for the desired product (M/Z = 578 BY positive ion CI AND M Z = 576 by negative ion CI).
Example 43 6-Memoxy-S-(4-memyl-pipera2in-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin- 1 -yl-phenyl)-amide.
The 4-[4-({ 1 -[6-Methoxy-8-(4-methyl-piperazm- 1 -yl)-4-oxo-4H-chromen-2-yl]- memanoyl}-amino)-phenyl]-piperazine-l-carboxylic acid ferf-butyl ester (Example 42) (0.792 gram, 1.37 mmol) was placed in a 50 ml round flask under nitrogen and it was dissolved in 15 ml of methylene chloride. The solution was treated with 15' ml of trifluoroacetic acid (195 mmol) to give a dark solution and it was stirred at room temperature for 18 hours. It was 0 concentrated to give a brown foam. The foam was treated with 30 ml of saturated aqueous sodium bicarbonate and it was stirred at room temperature as a yellow solid formed. The solid was filtered off, washed several times with water, air dried and dried under high vacuum (0.1 mm Hg pressure) to give 0.493 gram.of a yellow solid, M.P. = 203.6-204.7 C.
Proton MR and CI mass spectral analyses were consistent for the desired product (m/z = 478 by positive ion CI and m/z = 476 by negative ion CI).
Example 44-54 The following examples were prepared in parallel by acylation of 6-methoxy-8-(4- methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin- 1 -yl-phenyl)- amide (Example 43) in an Argonaut Quest synthesizer.
The piperazine side chain was derivatized in parallel fashion using eleven different commercially available acylating and sulfonating reagents. The resins used were Argonaut Tech polystyrene amine resins. Each 5 ml Quest tube was charged with 0.010 gram (0.021 mmol) of the starting N-H piperazine and 3ml of methylene chloride followed by 4 equivalents (0.08 mmol) of PS-DEEA resin (cm^opropylbenzylamine PS resin) to scavenge " HCI. Each tube was then treated with an acyl chloride, sulfonyl chloride, or isocyanate (2 . equivalents of each) followed by a little more methylene chloride. The tubes were sealed under nitrogen, and stirred for 3 hours at room temperature. The mixtures were then opened and treated with about 4 equivalents (0.08 mmol) of PS-trisamine resin (primary amine PS resin) to scavenge any excess acylating or sulfonating reagent. The mixtures were sealed and stirred for 1.5 hours and then filtered directly into vials and concentrated to give the products. The products were characterized by HPLC mass spectral analysis and were found to be greater than 90% pure by HPLC. The compounds were submitted to the 5-HTlb binding assay for determination of 5-HT receptor binding affinities and selectivities.
Example 44 6-Memoxy-8-(4-memyl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4- propionyl-piperazin- 1 -yl)-phenyl] -amide.
This compound was prepared from 6-memoxy-8-(4-memyl-piperazin-l-yI)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-arnide (Example 43) and commercially available propionyl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at m/z =534 by positive ion CI Example 45 6-Methoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-ethane sulfonyl-piperazm-l-yl)-phenyl]-amide. MS - base peak at m/z =570 by positive ion CI This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazm-l-yl-phenyl)-amide (Example 43) and commercially available ethanesulfonyl chloride (Aldrich) via the parallel synthesis described, above.
Example 46 6-Memoxy-8-(4-memyl-pipera2in-l-yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4- dimethyl sulfamoyl-piperazin- 1 -yl)-phenyl]-amide.
This compound was prepared from 6-memoxy-8-(4-memyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide (Example 43) and commercially available dimethylsulfamoyl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at m/z =585 by positive ion CI Example 47 • 4-[4-({ 1 -[6-Methoxy-8-(4-methyl-piperazin-l -yl)-4-oxo-4H-chromen-2-yl]-methanoyl}- ajtnino)-phenyl]-piperazine-l-carboxylic acid dimethylamide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide (Example 43) and commercially available dimethylcarbamyl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at m/z =549 by positive ion CI Example 48 4-[4-({ l-[6-Methoxy-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromen-2-yl]-memanoyl^ amino)-phenyl]-piperazme-l-carboxylic acid ethylamide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carbox}4ic acid (4-piperazin-l-yl-phenyl)-arnide (Example 43) and commercially available ethyl isocyanate (Aldrich) via the parallel synthesis described above.
MS - base peak at m/z =549 by positive ion CI.
Example 49 . 4-[4-({ l-[6-Methoxy-8-(4-memyl-piperazm-l-yI)-4-oxo-4H-cl^omen-2-yl]-methanoyl}-amino)-phenyl]-piperazine-l-carboxylic acid cyclohexylamide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide (Example 43) and commercially available cyclohexyl isocyanate (Aldrich) via the parallel synthesis described above. MS -base peak at m/z =603 by positive ion CI Example 50 4-[4-({ I-[6-Memoxy-8-(4-memyl-piperazdn-l-yl)-4-oxo-4H-cbxomen-2-yl]-methanoyl}- amino)-phenyI]-piperazine- 1 -carboxylic acid cyclopentylamide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide (Example 43) ahd commercially available cyclopentanecarbonyl chloride (Aldrich) via the parallel synthesis described above. . MS - base peak at m/z =574 by positive ion CI.
Example 51 6-Memoxy-8-(4-me&yl-piperazm-l-yl)-4-oxo-4H-c]iromene-2-carboxylic acid {4-[4-(1-' pyiTolia n-l-yl-methanoyl)-pipera2in-l-yl]-phenyl}-amide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l -yl)-4-oxo-4H-chromene-2 -carboxylic acid (4-piperazm-l-yl-phenyI)-amide (Example 43) and commercially available 1-pyrroUdinecarbonyl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at m/z =575 by positive ion CI.
Example 52 6-Memoxy-8-(4-memyl-piperazin-l-yl)^4-oxo-4H-chromene-2-carboxylic acid {4-[4- (propane-2-sulfonyl)-piperazin- 1 -yl]-phenyl } -amide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l -yI)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazm-l-yl-phenyl)-amide (Example 43) and commercially available isopropylsulfonylonyl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at mlz =584 by positive ion CI.
Example 53 6-Methoxy-8-(4-methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-carboxylic acid {4-[4-(2-methyl-propanoyl)-piperazin-l-yl]-phenyl}-amide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide (Example 43) and commercially available isobutyryl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at m/z =548 by positive ion CI.
Example 54 6-Memoxy-8-(4-memyI-piperazin-l-yl)-4-oxo-4H-cbxomene-2-carboxylic acid {4-[4-(l- moipholm-4-yl-me1ianoyl)-piperazin-lryl]-phenyl}-arnide.
This compound was prepared from 6-methoxy-8-(4-methyl-piperazin- 1 -yI)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide (Example 43) and commercially available morpholine-4-carbonyl chloride (Aldrich) via the parallel synthesis described above. MS - base peak at m/z =591 by positive ion CI.
Example 55 6-Fluoro-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide. - This compound was prepared from 6-Fluoro-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 3) and phenylamine (Reference Example 20) as prepared in Example 1, yielding a yellow solid. MS (M+H) m/z = 467 Example 56 6-Fluoro-8 -(4-methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-caiboxylic acid [4-(4- methanesulfonyl-piperazin- 1 -yl)-phenyl]-amide.
This compound was prepared from 6-Fluoro-8-(4-memyl-piperazin-l-yI)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 3) and 4-(4-methanesulfonyl- piperazm-l-yl)-phenylamine -(Reference Example 12) as prepared in Example 1, yielding a yellow solid. MS (M+H) m/z = 544 Example 57 6-Fluoro-8-(4-memyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-acetyl- piperazin- 1 -yl)-phenyl] -amide .
This compound was prepared from 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 3) and l-[4-(4-amino- phen3'l)-piperazin-l-yl]-ethanone (Reference Example 11) as prepared in Example 1, yielding a yellow solid. MS (M+H) m/z = 508 Example 58 6-Fluoro-8-(4-methyl-piperazin^l -yl)-4-oxo-4H-chromene-2-carboxylic acid (3-chloro-4- moφholin-4- l-phen l)-arnide . 6-Fluoro-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 3) (150 mg, 0.43 mmol), 1-hydroxybenzotriazole (140 mg, 0.9 mmol), 0-(lH-Benzotriazdl-l-yl)-N,N,N^N'-pentamethylene-uronium tetrafluoroborate (290 mg, 0.9 mmol), 4-(dimemylamino)pyridine (10 mg, catalytic), triethyla ine (0.2 mL, 1.5 mmol), and commercially available 3-chloro-4-morpholin-4-yl- phenylamine (Maybndge) were dissolved in dimethylformamide (2.5 mL) and stirred at room temperature overnight. At 17 h, water (20 mL) was added and the resulting mixture was stirred for 15-30 min. The mixture was vacuum-filtered and the residue washed with water and air-dried to yield a yellow powder (220 mg = quantitative yield). LC/MS - m/z = 501.5 Example 59 6-Fluoro-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (3-fluoro-4-morpholin-4-yl-phenyl)-amide.
This compound was prepared from 6-Fluoro-8-(4-memyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 3) and 3-fluoro-4-morpholin-4-yl-phenylamine (Reference Example 16) as prepared in Example 58, yielding a yellow solid (210 mg = 99%). LC/MS - m/z = 485.5 Example 60 6-Fluoro-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (3-cyano-4- morpholin-4-yl-plienyl)-amide.
This compound was prepared from 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 3) and 5-armno-2-morphoIin- 4-yl-berLzonitrile (Reference Example 15) as prepared in Example 58, yielding a yellow solid (210 mg = 99%). LC/MS - m/z = 492.5 Example 61 6-Fluoro-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-( 1 -moφholiD-4-yl-metha oyl)-phenyl]-amide.
This compound was prepared from 6-Fluoro-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 3) and l-(4-amino-phenyI)-l -morpholin-4-yl-methanone (Reference Example 14) as prepared in Example 58, yielding a yellow solid (220 mg = quantitative yield). LC/MS - m/z = 495.5 Example 62 6-Memyl-8-(4-memyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-morpholin-4- yl-phenyl)-amide.
This compound was prepared from 6-Merayl-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 4) and 4-morpholin-4-yl- phenylamine (Reference Example 20) as prepared in Example 1, yielding a. yeEow solid. LCMS - m/z = 463.6 Example 63 6-Memyl-8-(4-methyl-piperazm-l-yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(l- morpholin-4-yl-methanoyl)-phenyI]-amide.
This compound was prepared from 6-Methyl-8-(4-methyl-piperazin-l -yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 4) and l-(4-amino-phenyl)-l- morpholin-4-yl-methanone (Reference Example 14) as prepared in Example 1, yielding a yellow solid. LCMS - m/z = 491.6 · Example 64 6-Methyl-8-(4-methyl-piperazin-l -yl)-4-oxo-4H-chromene-2-carboxylic acid (3-fluoro-4-morphoIin-4-yl-phenyl)-amide.
This compound was prepared from 6-Methyl-8-(4-methyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 4) and 3-fluoro-4-morpholin 4-yl-phenylamine (Reference Example 16) as prepared in Example 1, yielding a yellow solid. LCMS - m/z = 504.5 Example 65 6-CMoro-8-(4-memyl-piperazm-l-yl)-4-bxo-4H-chromene-2-carboxylic acid (4-morpholin-4- yl-phenyl)-amide.
This compound was prepared from 6-chloro-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 5) and 4-morpholin-4-yl- phenylamine (Reference Example 20) as prepared in Example 1, yielding a yellow solid. LCMS - m/z = 483.3 Example 66 -Methyl-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-morpholin-4-yl-phenyl)-arnide.
This compound was prepared from 5-methyl-8-(4-methyl-piperazm- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride (Reference Example 6) and phenylamine (Reference Example 20) as prepared in Example 1, yielding a yellow solid (116 mg = 84%) LCMS- m/z = 463.5 Example 67 -Methoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-morphoIin- 4-yl-phenyl)ramide.
This compound 'was prepared from 5-methoxy-8-(4-methyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxyIic acid hydrochloride (Reference Example 7) and 4-morpholin-4-yl- phenylamine (Reference example 20) as prepared in Example 1, yielding a yellow solid (149 mg = 50%) LCMS - m/z = 479.4 The following additional examples incorporate 4-substitated piperazine-l-yl-phenyl amides similar in structure to Examples 44-54 Example 68 6-Methoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid {4-[4-(3-hydroxy-propanoyl)-piperazin-l-yl]-phenyl}-amide. 6-Methoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin- 1 -yl-phenyl)-amide (Example 43) ( 1.5 gram, 2.12 mmol) was placed in a 100 mL flask with 50 mL of CH2CI2. This suspension was treated with triemylamine (4 equivalents, 1.2 mL, 8.5 mmol) and β-propionylactone (0.2 mL, 3.2 mmol) and the reaction stirred at room temperature for 2 hours, then heated to 50°C for 2 hours. Then 0.8 mL more of b-propionylactone was added and the reaction heated for 4 hours more. The reaction was - Ill - allowed to cool to room temperature and then concentrated (1 mm Hg pressure). The concentrate was treated with saturated aqueous sodium bicarbonate and the resulting solid collected by vacuum filtration. The residue was purified by chromatography on silica eluting with 2% methanol in chloroform, then concentrated (1mm Hg pressure). Then triturated with 5 either to yield a yellow powder with was dried under high vacuum for 48 h at 50°C (100 mg) LC S - mlz 550, mp = 195-197°C.
-Example 69 4- [4-( { 1 -[6-Fluoro- 8 -(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromen-2-yl]-methanoyl } -amino)-10. phenyl]-piperazine-l-carboxylic acid iert-butyl ester.
This compound was prepared from 6-Fluoro-8-(4-metbyl-piperazin-l-yl)-4-oxo-4H- chromene-2-carboxylic acid hydrochloride (Reference Example 3) and 4 -(4-Arnino-phenyl)- piperazine-l-carboxylic acid terf-butyl ester (Reference Example 17) according to the method . of Example.42 to yield (1.65 grams, 64%) of a yellow powder LCMS - m/z = 556; mp = 15 219-220°C.
Example 70 4-[4-({ l-[6-Fluoro-8-(4-m'etiiyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4- piperazin-1 -yl-phenyl)-amide. 0 This compound was prepared from 4-[4-({ 1 -[6-Fluoro-8-(4-methyl-piperazin-l -yl)-4- oxo-4H-ckomen-2-yl]-meliLanoyl}-amino)-phenyl]-piperazine-l -carboxylic acid ier/-butyl ester, as prepared in Example 69, using the method of Example 43 to yield a yellow solid LCMS - m/z = 466.
Example 71 6-Fluoro-8-(4-memyl-piperazm-l-yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-ethane sulfohyl-piperazin-l-yI)-phenyl]-amide. . 4-[4-({ l-[6-Fiuoro-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chro nene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide ditrifluoroacetate (the free acid of which was prepared as in Example 70) (4.0 grams, 5.77 mmol) was placed in a flask with 50 mL of CH2C12 and triethylamine (3.2 mL and 23 mmol) and ethylsulfonyl chloride was added (0.6 mL, 6.35 mmol) portionwise (0.1 mL at a time) over' 15 minutes and allowed to stir at room temperature for 20 hours. The reaction was concentrated (1 mm Hg pressure) and then saturated aqueous sodium bicarbonate was added and extracted with CHC¾. The organic fractions were combined, washed with saturated sodium chloride, dried (MgS0 ) concentrated (1 mm Hg pressure) to give a yellow solid which was recrystallized from ' methanol to give 1.33 grams of product LCMS - m/z = 558, mp = 233-234°C.
Example 72 6-Fluoro-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-propionyl-piperazin-l-yl)-phenyl] -amide. 4-[4-({ l-[6-Fluoro-8-(4:memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin-l-yl-phenyI)-amide ditrifluoroacetate (the free acid of which was prepared as in Example 70) (0.69 grams, 1,00 mmol) was placed in a flask with 25 mL of CH2C12 and rxiemylarnine (0.56 mL and 4 mmol) and propionyl chloride was added (0.95 mL, 1.1 mmol) and the reaction allowed to stir at room temperature for 20 hours. The residue was purified by chromatography on silica eluting with 2% methanol in chloroform, then concentrated (1mm 5 Hg pressure). The residue was triturated with either then digested with CHCI3 and the CHCI3 concentrated to yield a yellow powder which was dried under high vacuum for 48 h at 45°C (260 mg) LCMS - mlz = 522.
Example 73 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid {4-[4-(3- hydroxy-propanoyl)-piperazin-l-yl]-phenyl}-amide.
This compound was prepared from 6-Fluoro-8-(4-methyl-piperazin-l-yI)-4-oxo-4H- chromene-2-carboxylic acid (4-piperazin-l-yl-phenyl)-amide and β-propionylactone using the ' method described above i Example 68 to yield 65 mg of a yellow powder LCMS - m/z = 15 538, mp = 195-199°C. · The following exemplifies a. substituted chromene-2-"reverse amide" (or substituted chromene-2-yl-benzamide).
Example 74 0 N-[8-(4-Methyl-piperazin- 1 -yl)-4-oxo-4H-chromen-2-yl]-4-morpholin-4-yl-benzamide. 8-(4-Methyl-piperazin- 1 -yI)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride Reference Example 1 (227 mg, 0.69 mmol), triethylamine (2 equivalents, 1.389 mmol, 0.193 mL) and diphenylphosphoryl azide (0.69 mmol, 0.15 mL) were stirred in toluene (10 mL) at 65°C for 30 minutes. The reaction was allowed to cool to 22°C and 4-morpholinobenzonoic acid (0.7 mmol, 145 mg), more triethylamine (0.051 mL, 0.7rnmol), and CH3CN (5 mL) were added and the reaction heated to reflux for 1 hour. The reaction was concentrated (1 mm Hg 5 pressure) the residue was partitioned between IN methanesufonic acid and ether. The acid layer was then basified with solid K2CO3 and the product extracted in to CHCI3. The. organic layer was dried (M S04) and concentrated under reduced pressure to leave a yellow solid which was further purified with silica chromatography using CHCI3 to 4% CH3OH in CHCI3. Concentration of the fractions containing product yielded 13 mg of product LC/MS - m/z = 10 449.
Enantiomers of 8-(4-Methyl-piperazin-l -yl)-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyI)-amide. · Example 75 racemic-8-(4-Methyl-piperazin- 1 -yl)-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyl)- amide. racemic-8-(4-Methyl- 1 -piperazin- 1 -yl)-chrbman-2-carboxylic acid hydrochloride (Example 75a) (1.04 mmol) was dissolved in anhydrous N,N-dimethylformamide (40 ml) and the following were added in order: HOBt (0.17 g, 1.14 mmol), TBTU (0.37 g, 1.14 mmol) 0 then triethylamine (0.6 ml, 4.2 mmol). After stirring for 5 min at' room temperature, 4-(4- mo holmyl)a iline (reference example 20) (0.185 g, 1.14 mmol) was added and the reaction stirred overnight at room temperature.
The solution was concentrated in vacuo, the remains were partitioned between chloroform / saturated sodium bicarbonate, extracted (x3) with chloroform, dried (MgSO^ and concentrated in vacuo to give the crude product.
The crude product was chroma to graphed on a Waters Delta Prep 4000 using 1 PrepPak cartridge (Porasil 37-55μιη 125 A) eluting with 2.5 % methanol / chloroform. The product was collected to give a yellow oil. Ethyl acetate was added to the oil. The solution was refluxed then cooled the yellow solid was filtered to give 55 mg (12% yield) of racemic-8-(4- methyl-piperazin-l-yl)-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyI)-amide (mp 215-216 °C). The mother liquor contained 76 mg that was used in the chiral separation described below. LC/MS (M+l) m/z = 437.
Example 75a racemic-8-(4-Methyl- 1 -piperazin- 1 -yl)-chroman-2-carboxylic acid hydrochloride.
Ethyl.8-(4-methyl- 1 -piperazin- 1 -yl)-4-oxo-4H-chromen-2-carboxylate (Reference Example 1) (0.74 g, 2.3 mmol) was dissolved in glacial acetic acid (50 ml) and 10 % palladium on carbon (80 mg) was- added. The mixture was hydrogenated on a Paar apparatus (50 psi) at 70 °C for 3 h. Then, concentrated HCl and 10 % palladium on carbon (100 mg) were added and the mixture was again subjected to hydrogenation (50 psi) at 70 °C for lh.
The reaction was allowed to cool, the catalyst was filtered and the solution was concentrated in vacuo. Toluene was repeatedly added and the solution concentrated to give racemic-8-(4-. Methyl- 1 -piperazin- l-yl)-chroman-2-carboxylic acid hydrochloride as a foam that was used without further purification in the next reaction. LC/MS (M+l) m/z = 277.
Example 76 (+)-8-(4-Methyl-piperazin-l-yI)-chroman-2-carboxylic acid (4-mo holin-4-yl-phenyI)-amide.
The enantiomers of racemic-8-(4-Methyl-piperazin-l-yl)-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide (Example 75) (0.52 g, 1.19 mmol) were separated by the use of a chiral column (ChiralPak AD, 5 cm X 50 cm, 20 μ). The faster (+) isomer (example 76) was eluted with 45 % isopropanol / hexane and the slower (-)isomer (example 77) was eluted with 75 % isopropanol / hexane.
The faster (+) isomer (example 76) was obtained as a white solid (250 mg, mp 206-207 °C, cxD + 92.66 in dichloromethane). LC/MS (M+l) m/z = 437.
Example 77 (-)-8-(4-Methyl-piperazin- 1 -yl)-chroman-2-carboxylic acid (4-morpholm-4-yl-phenyl)-amide. The enantipmers of racernic-8-(4-Methyl-piperazm-l -yl)-chroman-2-carboxylic acid ( -mo^holin-4-yl-phenyl)-amide (Example 75) (0.52 g, 1.19 mmol) were separated by the use of a chiral column (ChiralPak AD, 5 cm X 50 cm, 20 μ). The faster (+) isomer (example 76) was eluted with 45 % isopropanol / hexane and the slower (-)isomer (example 77) was eluted with 75 % isopropanol / hexane.
The slower (-) isomer (example 77) was obtained as obtained as a light purple solid (260 mg, mp 205.5-207 °C, aD - 91.08 in dichloromethane). LC/MS (M+.l) m/z = 437. ' Enantiomers of 8-(4-methyl-piperazin-l-yl)-4-oxo-chroman-2-carboxylic acid (4-moφholin-4- l- henyI)-amide .
Example 78 racemic-8-(4-methyl-piperazin- 1 -yl)-4-oxo-chroman-2-carboxylic acid (4-morpholin-4-yI-phenyl)-amide.
Racemic-8-(4-methyl- 1 -piperazin- 1 -yl)- 4-oxo-chroman-2-carboxyIic acid hydrochloride (Example 78a) (1.04 mmol) was dissolved in anhydrous N,N-dimethylformamide (40 ml) and the following were added in order: HOBt (0.17 g, 1.14 mmol), TBTU (0.37 g, 1.14 mmol) then triemylartiine (0.6 ml, 4,2 mmol). After stirring for 5 rnin at room temperature, 4-(4-morpholinyl)aniline (reference example 20) (0.185 g, 1.14 mmol) was added and the reaction stirred overnight at room temperature.
The solution was concentrated in vacuo, the remains were partitioned between chloroform / saturated sodium bicarbonate, extracted (x3) with chloroform, dried (MgS04) and concentrated in vacuo to give the crude product The crude product was chromatographed on a Waters Delta Prep 4000 using 1 PrepPak cartridge (Porasil 37-55jim 125 A) eluting with 2.5 % methanol / chloroform. The product was collected to give a yellow oil. Ethyl acetate was added to the oil..The solution was refluxed then cooled the yellow solid was filtered to give 55 mg (12% yield) of racemic- 8-(4-methyl-piperazin- 1 -yl)-4-oxo-chroman-2-carboxylic acid (4-mo hόlin-4-yl-pheny-)- amide (mp 215-216 °C). The mother liquor contained 76 mg that was used in the chiral separation described below. LC/MS (M+l) m/z = 451.
Example 78a racemic-8-(4-Methyl-l-piperazin-l-yl)- 4-oxo-chroman-2-carboxylic acid hydrochloride. racemic-Ethyl-8-(4-methyl- 1 -piperazinyl)- 4-oxo-chroman-2-carboxylate (Example 78b) (0.33 g, 1.04 mmol) was dissolved in 6 M HC1 (20 ml) and heated, to 100 °C for 1.5 h. The reaction was allowed to cool. The solution was concentrated in vacuo and anhydrous toluene was added (x3) and the solution was again concentrated in vacuo to give racemic-8- (4-Methyl-l-piperazin-l-yI)- 4-oxo-chroman-2-carboxylic acid hydrochloride as a yellow foam (0.44 g, quantitative yield) that was used as is in the next reaction. LC/MS (M+l) m/z = 291.
Example 78b racemic-Ethyl-8-(4-methyl-l-piperazin-l-yl)- 4-oxo-chroman-2-carboxylate.
Raceniic-Emyl-8-(4-methyl-l-piperazin-l-yl)-4-hydroxy-chroman-2-carboxylate (Example 78c) (0.43 g, 1.3 mmol) was dissolve in anhydrous dichloromethane (35 ml) and manganese dioxide (1.2 g, 13 mmol) was added. The reaction stirred at room temperature overnight.
The reaction was filtered through diatomaceous earth and the solvent was removed in vacuo to give racerdc-Ethyl-8-(4-methyl-l-piperazin-l-yl)- 4-oxo-chroman-2-carboxylate as a white solid (0.37 g, 86 % yield) that was used as is in the next reaction. GC/MS (EI, M+) m/z = 318.
Example 78c racemic-Ethyl-8-(4-methyl-l-piperazin-l-yl)- 4-hydroxy-chroman-2-carboxylate.
Ethyl 8-(4-methyl- 1 -piperazin- 1 -yI)-4-oxo-4H-chroman-2-carboxylate (reference example 1) (0.48 g, 1.5 mmol) was dissolved in glacial acetic acid (50 ml) and ] 0 % palladium on carbon (100 mg) was added. The mixture was hydrogenated on a Paar apparatus (50 psi) at 70 °C for 3 h.
The reaction was allowed to cool, the catalyst was filtered and the solution was concentrated in vacuo. Ethyl acetate / saturated sodium bicarbonate was added to the remains and the mixture was extracted (x3) with ethyl acetate, dried (MgSC^) and stripped to give racemic-Ethyl-8-(4-methyl-l-piperazin-l-yl)- 4-hydroxy-chroman-2-carboxylate (0.43 g, 90 % yield) as a yellow oil. GC/MS (EI, M+) m/z = 320.
Example 79 8-(4-Methyl-piperazin- 1 -yl)-4-oxo-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyl)- amide (faster running isomer).
The enantiomers of the racemic-8-(4-methyl-piperazin-l-yl)-4-oxo-chroman-2- carboxylic acid (4-mo holin-4-yl-phenyl)-amide (Example 78) (100 mg, 0.22 mmol) were separated by the use of a chiral column (ChiralPak AD, 5 cm X 50 cm, 20 μ). The isomers were eluted with a gradient of 35-55 % isopropanol / hexane. The faster isomer was obtained as a light yellow solid (40 mg, mp 216 °C dec.) LC/ S (M+l) m/z = 451.
Example 80 8^(4-Memyl-piperazin-l-yl)-4-oxo-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide (slower running isomer).
The enantiomers of the racemic-8-(4-methyl-piperazin-l-yl)-4-oxo-chroman-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide(l 00 mg, 0.22 mmol) were separated by the use of a chiral column (ChiralPak AD, 5 cm 50 cm, 20 μ). The isomers were eluted with a gradient of 35-55 % isopropanol / hexane. The slower isomer was obtained as an off white solid (32 mg, mp 215 °C dec.) LC/MS (M+l) m/z = 451.
Example 81 6-Fluoro-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-piperazin-l - yl-phenyl)-amide (Example 71) (150 mg, 0.216 mmol) was placed in a 50 mL flask with 10 mL of CH2CI2. This suspension was treated with triemylamine (0.1 mL, 0.67 mmol) and ethylisocyanate (0.21 mL, 18.7 mg, 0.26 mmol) and the reaction stirred at room temperature for 18 hours. The reaction was concentrated (1 mm Hg pressure) and the concentrate purified by chromatography on silica eluting with 1 % methanol in chloroform, then concentrated (lmm Hg pressure). Then triturated with either to yield a yellow powder with was dried under high vacuum for 48. h at 50°C (79 mg) LCMS - AP+ 537.4, mp = 23.6-238°C.
Example 82 6-Methoxy-8-(4-methyl-[l,4]dia2epan-l-yl)-4-oxo-4H-chromene-2-carboxylic acid (4-mo holin-4-yl-phenyI)-amide: Into a 100 mL round bottom flask equipped with a nitrogen inlet and magnetic stirrer is added 327 mg (0.89 mmol, 1.0 equiv.) of 6-Methoxy-8-(4-methyl-[l,4]diazepan-l-yl)-4-oxo-4H-chromene-2-carboxylic acid hydrochloride salt (Reference Example 23). This material is dissolved in 20 mL of DMF and then 189 mg (1.06 mmol, 1.2 equiv.) of 4-■ mo hoh o niIi e is added. To the stirred solution is quickly added simultaneously added . 568 mg (1.77 mmol, 2.0 equiv.) of TBTU and 239 mg (1.77 mmol, 2.0 equiv.) of HOBT. At this point 457 mg, 577 jiL (25.2 mmol, 4.0 equiv.) is added via syringe over 5 minutes. The reaction is allowed to stir at room temperature for 18 hrs, then is concentrated on a rotary evaporator under high vacuum in order to remove the DMF. The residue is triturated with methanol and the crude solids are recovered by filtration. These residues are then purified by flash chromatography using a gradient of 5-10% methanol in methylene chloride as eluent. The eluted material, which is obtained from chromatography, is concentrated, dried under high vacuum, suspended in methylene chloride, dried over K2CO3, concentrated, then crystallized from methanol to give the free base of the pure product as 345 mg (79%) of a yellow solid. Mass Spec: calc. for [C27¾2:FN405+H]+ Theor. m/z = 393; Obs. = 393 Example 83 6-Ethoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid (4-morpholin-4- yl-phenyl)-amide: Into a 100 mL flask equipped with a nitrogen inlet and magnetic stirrer is placed 133 mg (.748 mmol, 1.1 equiv.) of 4-morpholinoaniline, which is then dissolved in 20 mL of methylene chloride. To this rtiixtuie is then added 290 mg, 367 (2.24 mmol, 3.3 equiv.) of ethyldiisopropyl amine, followed by addition of a solution of 250 mg (0.68 mmol, 1.0 equiv.) of 6-emoxy-8-(4-memyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carbonyl chloride (Reference Example 23) which has been dissolved in 10 ml of methylene chloride. The reaction is allowed to stir for 4 hr, after which no further formation of product was seen by LC/MS. The crude reaction was concentrated on a rotary evaporator, then triturated with 10 mL of methanol. The crude solids were collected by filtration, then subjected to flash chromatography using a gradient of from 2 to 20% methanol in methylene chloride.
Recrystallization from methylene chloride and hexanes afforded 55 mg (16%) of the product as a yellow solid.
Mass Spec: calc. for [C27H32N 05+H]+ Theor. m/z = 493; Obs. = 493 Example 84 6-Ethoxy-8-(4-methyl-piperazin- 1 -yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-propionyl- piperazin-l-yl)-phenyl]-amide: ■ This compound was prepared from 250 mg (0.68 mmol, 1.0 equiv.) of 6-Ethoxy-8-(4- methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carbonyl chloride (Reference Example 23) and 10 175 mg (0.748 mmol, 1.1 equiv.) of l-[4-(4-Amino-phenyl)-piperazin-l-yl]-propan-l-one by an analogous procedure to that used to prepare the 4-morpholino aniline derivative, to give 45 mg (12%) of the desired product as a yellow solid.
Mass Spec: calc. for Example 85 6-Methoxy-4-oxo-8-piperazin- 1 -yl-4H-cbromene-2-carboxylic acid (4-morpholin-4-yl- phenyl)-amide: Into a 50 mL round bottom flask equipped with a reflux condenser, nitrogen inlet and magnetic stirrer is placed 50 mg (0.1 15 mmol, 1.0 equiv.) of 6-Methoxy-8-(4-methyl-0 piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid ( -moφholi -4- l-phenyl)-armde (Example 31) and 10 mL of 1, 2 dichloroethane. To this solution is then added via syringe 49 ■mg, 37 L (0.345 mmol, 3.0 equiv.) of 1-chloroethyl chloroformate. A precipitate forms, indicating formation of an intermediate. The reaction is heated to reflux for 3 days, whereupon an analysis of an aliquot by LC/MS indicates only a trace of product has formed. At this time 52 mg (0.345 rnrnol, 3.0 equiv.) of sodium iodide are added to the refluxing reaction. LC/MS analyses then progressively show formation of demethylated product over 5 additional days. The reaction is then cooled, concentrated on a rotary evaporator, then dried over K2CO3 as a suspension in methylene chloride containing methanol, removal of solids by filtration, followed by flash chromatography of the solution, using a gradient of 5 to 20% methanol in methylene chloride, gives 34 mg (64%) of the pure product as a reddish solid. Mass Spec: calc. for [CisHa^Os+H]* Theor. m/z = 465; Obs. = 465 Example 86 6-Hydroxy-8-(4-methyl-piperazin-l-yl)-4-ox0-4H-chromene-2-carboxyUc acid (4-morpholin- 4-yl-phenyl)-amide: Into a 50 ihL round bottom flask equipped with a reflux condenser, nitrogen inlet and magnetic stirrer is placed 50 mg (0.1 15 mmol, 1.0 equiv.) of 6-Methoxy-8-(4-methyl-piperazin-l-yI)-4-oxo-4H-chromene-2-carboxylic acid (Example 31) and 20 mL of methylene chloride. To this solution is added 1 mL of a IN solution of boron tribromide in methylene chloride. The reaction is stirred at room temperature for 2.5 days at which time it is complete by LC/MS. The reaction is concentrated on a rotary evaporator, then methanol is added. The methanol is concentrated and readded 5 times, until the BBr3 is removed as HBr and trimethyl borate. The solid hydrobromide salt residue, which is obtained, is >85% pure product by LC/MS. Mass Spec: calc. for [¾Η28Ν4θ5+Η]+ Theor. m z = 465; obs. = 465 Example 87 fMethod 1) 6-Memoxy-8-(4-memyI~[l,4]aaazepan-l-yl)-4-oxo-l,4-dm^ acid (4-morpholin-4-yl-phenyl)-amide.
. To a solution of 6-methoxy-8-(4-methyl-[l ,4]diazepan-l -yl)-4-oxo-l ,4-dihydro- quinoline-2-carboxylic acid (2.10 mmol) (Reference Example 25b) and diisopropylethyl amine (1.4 mL, 8.6 mmol) in 34 mL dimethylformamide was added TBTU (1.40 g, 4.36 mmol) and HOBt (0.588 g, 4.35 mmol) followed by the addition of 4-morphoHnoaniline (0.463 g, 2.60 mmol). The resulting dark brown solution was stirred' at room temperature under nitrogen for 19 hours. The reaction was concentrated in vacuo and the resulting crude product was taken up in methylene chlbride/methanol. Filtration of the resulting mixture afforded some product as a yellow solid. The filtrates were concentrated and partitioned between methylene chloride and saturated aqueous sodium bicarbonate. The organic layer was washed with saturated sodium bicarbonate, dried (MgS04), and concentrated under vacuum to afford a brown solid. This was suspended in methanol and filtered to afford the desired product as a yellow solid (0.714 g, 69%). Ή MR (300 MHz, DMSO, d6) δ 9.97 (bs, 1 H, NH), 7.67 (d, 2 H, J0= 8.8 Hz, ArHjr&iie , 7.47 (bs, 1 H, ArHj), 7.00 (s, 1 H, C=CH), 6.99 (d, 2 H, J0= 8.8 Hz, ΑιΗ^& Ή^), 6.71 (bs, 1 H, ArH7), 3.85 (s, 3 H, OCH3), 3.75 (t, 4 H, J= 4.6 Hz, OCH2CH2N), 3.70 (bs, 2 H, ArNC CHaCHaNCHa), 3.55 (bs, 2 H, ArNCH^CH2NC¾), 3.09 (t, 4 H, J= 4.6 Hz, OCH2CH2N), 2.95 (bs, 2 H, ' ArNCHzCHjNCHj), 2.73 (bs, 2 H, ArNC^CHaCH^NCT , 2.36 (s, 3 H, NCH3), 2.07 (bs, 2 H ArNC¾CH2CH2NCH3); Mass Spec: calc. for [C27H33N504+H]+ Theor. m/z = 492; Obs. 492.
Example 87 (Method 2) 6-Methoxy-8 -(4-methyl-[ 1 ,4] diazepan- 1 -yl)-4-oxo- 1 ,4-dihyclro-qxrinolme-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide.
A solution of 6-memoxy-8-(4-me&yl-[l,4]diazepan-l-yl^ emoxyme&oxy)-quiholine-2-carboxylic (Reference Example 27d) (0.989 g, 1.59 mmol) in 20 mL methanol was poured into 300 mL 0.05 N hydrochloric acid. The clear dark yellow solution became cloudy within 5 minutes. The mixture was stirred at room temperature for 45 minutes and then adjusted to pH 7 with 10% sodium hydroxide. The resulting yellow precipitate was isolated by filtration, Washed with water, and dried under high vacuum to afford the desired product as a yellow solid (0.629 g, 80%). Ή NMR (300 MHz, DMSO, d6) δ 9.97 (bs, 1 H,C(0) H), 7.67 (d, 2 H, J0= 8.8 Hz, ΑτΗ2·& He-), 7.47 (bs, 1 H, ArHj), 7.00 (s, 1 H, OCH), 6.99 (d, 2 Ή, J0= 8.8 Hz, Hs , 6.71 (bs, 1 H, ArH7), 3.85 (s, 3 H, OCH3), 3.75 (t, 4 H, J= 4.6 Hz, OCikCHjN), 3.70 (bs, 2 H, ArNCHzCHzCHzNCHa), 3.55 (bs, 2 H, ArNCHiCH2NCH3), 3.09 (t, 4 H, J= 4.6 Hz, OCH2CH2N), 2.95 (bs, 2 H, 2 H, Ar CH2CH2CH2 CH3), 2.36 (s, 3 H, NCHj), 2.07 (bs Mass Spec: calc. for · [C27H33N504+H]+ Theor. m/z = 492; Obs. = 492. Analysis for C27H33N5O4. l.OeqHCl. 0.3eqH2O: Calculated C 60.79 H 6.54 N 13,13. Found C 60.82 H 6.53 N 13.17.
Example 88 6-Meihoxy-8-(4-memyl-piperazin-l-yl)-4-oxo-l)4-dihydro-qumoune-2-carboxy-^ acid (4- morpholin-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-trimethylsilanyl- emoxymethoxy)-quinoline-2-carboxylic acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 1). A ' ■ yellow solid was obtained. Mass Spec: calc. for [C26¾ ι 5θ4+Η]+ Theor. m/z = 478; Obs. 478. ' Example 89 6-Memoxy-8-(4-memyl-piperazin-l-yl)-4-oxo-l,4-dmydro-quinoline-2-carboxylic acid [4-(4- propionyl-piperazin- 1 -yl)-phenyl]-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-trimethylsilanyl- ethoxymethoxy)-quinoline-2-carboxylic acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 1), except that .the amide was formed from l-[4-(4-amino-phenyl)-piperazin-l-yl]-propan-i-one. A yellow solid was obtained. Mass Spec: calc. for [C29¾6N60 +H]+ Theor. m/z = 533; Obs. 533.
Example 90 6-Fluoro-8 -(4-methyl-piperazin- 1 -yl)-4-oxo- 1 ,4-dmydro-qumolme-2-carboxylic acid (4-mo holin-4- l-phenyI)-amide The title compound was prepared from 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo- l,4-dmydro-quinoline-2-carboxylic acid hydrochloride salt (Reference Example 26) using the procedure described in Example 87 (Method 1). After chromatography, it is then crystallized from methanol to give the pure product as 150 mg (55%) of a yellow solid. Mass Spec: calc. for [C25H28FNs03+H]+ Theor. m/z = 466; Obs, = 466.
Example 91 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo-l34-dmydro-quinoline-2-carboxyIic acid [4-(4- propionyl-piperazin-l-yl)-phenyl]-amide.
The title compound was prepared from 6-Fluoro-8-(4-methyl-piperazin-l-yl)-4-oxo- l,4-dihydro-quinoline-2-carboxylic acid hydrochloride salt (200 mg, 0.59 mmol) (Reference Example 26) using the procedure described in Example 87 (Method 1). 31% yield. Mass Spec: calc for [C2sH33FN603+H]+ Theor. m/z = 521; Obs. = 521.
Example 92 8-[(2-Dimethylaiiiino-emyl)-me& carboxylic acid (4-morpholin-4-yl-phenyI)-amide.
The title compound was prepared from 8-bromo-6-methoxy-4-(2-trimethylsilanyl-emoxymemoxy)-quinoline-2-carboxylic acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 2), using Ν,Ν,Ν'-trimethyl ethylenediamine for the Pd catalysed coupling. A yellow solid was obtained. Mass Spec: calc. for [C26H33Nj0 +H]+ Theor. m/z = 480; Obs. = 480.
Example 93 8-[(3-Dimethylan ino-propyl)-methyl-amino]-6-methoxy-4-oxo-l ,4-dihydro-quinoline-2- carboxylic acid (4-morpholin-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-trimethylsilanyl- ethoxymethoxy)-quinoline-2-carboxyUc acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 2), using Ν,Ν,Ν'-trimethyl- 1 ,3-propanediamine for the Pd catalysed coupling. A yellow solid was obtained. Mass Spe 94; Obs. = 494.
Example 94 8-((3R)-(+)-3-Dimethylarnino-pyiTolidm -l-yI)-6-methoxy-4-oxo-l,4-dihydro-quinoline-2-carboxylic acid (4rmorpholin-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-trimethylsilanyl-ethoxymethoxy)-quinoline-2-carboxylic acid methyl (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 2), using (3R)-(+)-3-(dicQethylaiiiino)pynOlidine for the Pd catalysed coupling. A yellow solid was obtained. Mass Spec: calc. for Theor. m/z = 492; Obs. = 492.
Example 95 8-((3S)-(-)-3-Dimemylarnino-pyiTolidin -l-yl)-6-memoxy-4-oxo-l>4-dihydro-quinoline-2- carboxylic acid (4-morpholm-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-teimethylsilanyl- ' ethoxymethoxy)-quinoline-2-carboxylic acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 2), using (3S)-(-)-3-( Example 96 . 6-Methoxy-8-[methyl-( 1 -methyl -pyrrolidin-3 -yl)-amino] -4-oxo- 1 ,4-dihydro-quinoline-2-carboxylic acid (4-morpholin-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-trimethylsilanyl-ethoxymethoxy)-quinoline-2-carboxylic acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 2), using N,N'-dime±yl-3-aminopyrrolidine for the Pd catalysed coupling. A yellow solid was obtained. Mass Spec: calc for [C27H33N504+H]+ Theor. m/z = 492; Obs. = 492.
Example 97 8-[Ethyl-(l-ethyl-pyrrolidin-3-yl)-arnmo]-6-methoxy-4-oxo-l,4-d^ carboxylic acid (4-morpholin-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-(2-trimethylsilanyI- ethoxymethoxy)-quinoline-2-carboxylic acid methyl ester (Reference Example 24c) according to the procedures described in Reference Example 25a and in Example 87 (Method 2), using 3-diethylaminopyrrolidine for the Pd catalyzed coupling. A yellow solid was obtained. Mass Spec: calc. for [Cz^NsC +H]* Theor. m/z = 520; Obs. = 520.
Example 98 4-Dimethylamino-6-methoxy-8 -(4-methyl-piperazin- 1 -yl)-quinoline-2-carboxylic acid (4- ■ morpholin-4-yl-phenyl)-amide.
To a suspension of 8 -bromo-4-dimemylamino-6-memoxy-qumoline-2 -carboxylic acid (4-morpholin-4-yl-phenyl)-amide (Reference Example 28b) (139.9 mg, 0.288 mmol), N-methylpiperazine (48 |iL, 0.43 mmol), and 4 A sieves in 15 mL anhydrous toluene was added Pd2 (dba) 2 (15.3 mg, 16.7 μιηοΐ), ΒΓΝΑΡ (63.0 mg, 0.101 mmol) and cesium carbonate (0.436 g, 1.345 mmol). The resulting wine colored mixture was heated at reflux under nitrogen for 20 hours. The reaction mixture was cooled to room temperature and concentrated. The crude mixture was purified by flash chromatography on silica gel using a gradient of 100:0 to 95:5 methylene chloride methanol to afford the desired product as a yellow solid (96.9 mg, 67%). Ή NMR (300 MHz, DMSO, d6) δ 10.06 (s, 1 H, C(O)NH), 7.69 (d, 2 H, 6.95 (d, 1 H, Jm= 2.7 Hz, ArH^), 6.76 (d, 1 H, Jm= 2.7 Hz, ·ΑιΉ7), 3.90 (s, 3 H, OCH3), 3.75 (t, 4 H, J= 4.8 Hz, OCHzCHzN), 3.37 (bs, 4 H, ArNCHzCHzN), 3.10 (t, 4 H, J= 4.8 Hz, OCH2CH2N), 3.01 (s, 6 H, N(CHj) 2), 2.71 (bs, 4 H, Ar CHzCHj ), 2.35 (s, 3 H, R2NCHj ); Mass Spec.: calc. for [C28H36N603+H]+ Theor. m/z = 505; Obs. = 505.5.
Example 99 6-Methoxy-4-methylamino-8-(4-methyl-piperazin-l-yl)-quinoline-2-carboxylic acid (4- morpholin-4-yl-phenyl)-amide.
The title compound was prepared from 8-bromo-6-memoxy-4-oXo-l,4-dmydro- quinoline-2-carboxylic acid (Reference Example 27b) according to the procedure described for Example 98 using N-methyl amine to prepare 8-bromo-4-memylamino-6-methoxy-' quinoline-2-carboxylic acid (4-mo holin-4-yl-phenyl)-amide. A glassy orange solid was obtained. Mass Spec: calc. for [C^H^NeC +H] " Theor. m/z = 491; Obs. = 491.5.
Example 100 6-Fluoro-4-methoxy-8-(4-methyl-piperazin-l -yl)-quinoline-2-carboxylic acid (4-π-θφηο1-η-4-yl-phenyl)-arnide.
Into a 250 mL round bottom flask equipped with a nitrogen inlet and magnetic stirrer is added 2.0 lg (6.3 rnmol, 1.0 equiv.) of 6-Fluoro-4-methoxy-8-(4-methyl-piperazin-l-yI)- quinoline-2-carboxylic acid hydrochlori.de salt.. This material is dissolved in 20 mL of DMF and then 1.35 g (7.56 mmol, 1.2 equiv.) of 4-morpholinoaniline is added. To the stirred solution is quickly added simultaneously added 4.05g (12.6 mmol, 2.0 equiv.) of TBTU (2- (lH-benzotriazole-l-yl)-l,l,3,3tetramemyluroniumtetrafluoroborate) and 1.7 g (12.6 mmol, 2.0 equiv.) of HOBT (1-hydroxybenzotriaole hydrate). At this point 3.25 g, 4.11 mL (25.2 mmol, 4.0 equiv.) is added via syringe over 5 minutes. The reaction is allowed to stir at room temperature for 18 hrs, then is concentrated on a rotary evaporator under high vacuum in- order to remove the DMF. The residue is triturated with methanol and the crude solids are recovered by filtration. The material is then dissolved in methylene chloride and extracted with 10% sodium bicarbonate solution. The organic layer is dried and then concentrated. These residues are then purified by flash chromato raphy using a gradient of 5-10% methanol in methylene chloride as eluent. The material which is obtained from chromatography, is then crystallized from methanol to give the pure product as 2.83g (93%) of a yellow solid.
Mass Spec: calc. for [C26H3oFN 03+H]+ Theor. m/z = 480; Obs. = 4S0 Example 101 6-Fluoro-4-oxo-8-piperazin-l -yl-4H-chromene-2-carboxylic acid (4-morpholin-4-yI-phenyl)-ami.de: made according to the general method of Howarth et. al. Tetrahedron, 1 98, 54, 10899-10914.
Dry 6-floui -8-(4-methyl-piperazin-l-yl)-4-oxo-4H-chromene-2-carboxylic acid [4-(4-propionyl-piperazin-l-yl)-phenyl]-amide (Example 72)(1 g 1.9 mmol ) was added to 100 mL of rigorously dried 1 ,2-dichloroethane in a flask under N2 atmosphere and magnetic stirring. The rx ixture was cooled to 06C and freshly distilled 1-chloroethyl chloroformate (650 ul, 858 mg, 6 mmol, 3 eq) was added drop wise. The reaction was then heated under reflux for 5 hours at which time LC/MS revealed complete consumption of starting material. Nal (l , 1 eq) was added and heating continued for 2 days more. The reaction was then allowed to cool and filtered and evaporated to dryness under reduced pressure. MeOH (100 mL) was added and heated to reflux for 4h, filtered hot and evaporated to dryness. The product was isolated by chromatography using silica gel and CHC13/5% MeOH as an eluent. This gave 700 mg of the product HC1 salt as a yellow solid. LCMS - m/z = 508.
Material which is outside the scope of the claims does not constitute part of the claimed invention.

Claims (1)

1. CLAIMS A compound represented by the formula wherein R1 at each independently represented by optionally substituted optionally substituted cyano or A is optionally substituted optionally substituted optionally substituted or optionally substituted R2 is represented by or optionally substituted optionally substituted optionally substituted or n is 3 or a heterocyclic R6 is or Y is NACH2 or a 133 R7 is a monocyclic or bicyclic aromatic ring or a heterocycle optionally substituted by one or substituents selected from and wherein R7 is connected to Y either by a single bond or by a ring R8 is a single bond as tether from R7 to heterocycle connected to R7 by a ring fusion or a single bond as R9 optionally substituted optionally substituted optionally substituted optionally substituted optionally substituted thiomorpholinyl or is optionally substituted optionally substituted or is or a pharmaceutically acceptable salt of said A compound recited in claim 1 for use in the treatment of migraine in a human or animal in need of such The use of the compound recited in claim 1 in the preparation of a medicament for the treatment of A pharmaceutical composition comprising a compound as recited in Claim 1 or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable 134 The compound as recited in claim 1 is halogen or methoxy at the 6th position of the bicyclic and is ethyl or methoxy at the 5th position of the bicyclic and is hydrogen at the 7th position of the bicyclic The compound as recited in claim 5 is fluorine at the 6th position of the bicyclic The compound as recited in claim 1 R2 is methyl The compound as recited in claim 1 R6 is The compound as recited in claim 1 Y is The compound as recited in claim 1 R7 is phenyl substituted with The compound as recited in claim 1 R is a single bond as The compound as recited in claim 1 R9 is a heterocyclic moiety attached to R8 by The compound as recited in claim 12 R9 is a morpholine attached to R8 by The compound as recited in claim 12 135 R is a piperazine attached to R by nitrogen and optionally substituted on the other nitrogen with The compound as recited in claim 1 is AOH or wherein A is represented by methyl or The compound as recited in claim 1 is halogen or methoxy at the 6th position of the bicyclic and is ethyl or methoxy at the 5th position of the bicyclic and is hydrogen at the 7th position of the bicyclic R2 is methyl R6 is Y is R7 is phenyl substituted with R8 is a single bond as is a heterocyclic moiety attached to R by and 1 is AOH or wherein A is represented by methyl or The compound as recited in claim 16 R1 is fluorine at the 6th position of the bicyclic The compound as recited in claim 16 R9 is a morpholine attached to R8 by The compound as recited in claim 18 R1 is fluorine at the 6th position of the bicyclic The compound as recited in claim 16 R9 is a piperazine attached to R8 by nitrogen and optionally substituted on the other nitrogen with ώ at the 6th v LUZZATT0 LUZZAJTO insufficientOCRQuality
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MY138263A (en) 2009-05-29
JP4280068B2 (en) 2009-06-17
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NO20033205L (en) 2003-09-02
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