IL129003A - שיטה לגילוי קולורימטרי סלקטיבי עבור קטיונים בתמיסות מימיות - Google Patents
שיטה לגילוי קולורימטרי סלקטיבי עבור קטיונים בתמיסות מימיותInfo
- Publication number
- IL129003A IL129003A IL12900399A IL12900399A IL129003A IL 129003 A IL129003 A IL 129003A IL 12900399 A IL12900399 A IL 12900399A IL 12900399 A IL12900399 A IL 12900399A IL 129003 A IL129003 A IL 129003A
- Authority
- IL
- Israel
- Prior art keywords
- peptides
- vesicles
- colorimetric detection
- vesicle
- colorimetric
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 35
- 150000001768 cations Chemical class 0.000 title claims description 15
- 239000007864 aqueous solution Substances 0.000 title claims description 13
- 150000003904 phospholipids Chemical class 0.000 claims abstract description 20
- 239000011159 matrix material Substances 0.000 claims abstract description 18
- 230000007704 transition Effects 0.000 claims abstract description 10
- 230000000712 assembly Effects 0.000 claims abstract description 6
- 238000000429 assembly Methods 0.000 claims abstract description 6
- 239000000470 constituent Substances 0.000 claims abstract description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 49
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 40
- 229920000015 polydiacetylene Polymers 0.000 claims description 36
- 239000002555 ionophore Substances 0.000 claims description 26
- 230000000236 ionophoric effect Effects 0.000 claims description 26
- 239000012528 membrane Substances 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 18
- 150000002500 ions Chemical class 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 17
- 230000008859 change Effects 0.000 claims description 13
- 230000003115 biocidal effect Effects 0.000 claims description 10
- 230000003993 interaction Effects 0.000 claims description 9
- 239000000232 Lipid Bilayer Substances 0.000 claims description 7
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 230000010399 physical interaction Effects 0.000 claims description 6
- 229930191564 Monensin Natural products 0.000 claims description 5
- GAOZTHIDHYLHMS-UHFFFAOYSA-N Monensin A Natural products O1C(CC)(C2C(CC(O2)C2C(CC(C)C(O)(CO)O2)C)C)CCC1C(O1)(C)CCC21CC(O)C(C)C(C(C)C(OC)C(C)C(O)=O)O2 GAOZTHIDHYLHMS-UHFFFAOYSA-N 0.000 claims description 5
- 108010067973 Valinomycin Proteins 0.000 claims description 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 5
- FCFNRCROJUBPLU-UHFFFAOYSA-N compound M126 Natural products CC(C)C1NC(=O)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)C)OC(=O)C(C(C)C)NC(=O)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)C)OC(=O)C(C(C)C)NC(=O)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)C)OC1=O FCFNRCROJUBPLU-UHFFFAOYSA-N 0.000 claims description 5
- 229960005358 monensin Drugs 0.000 claims description 5
- GAOZTHIDHYLHMS-KEOBGNEYSA-N monensin A Chemical compound C([C@@](O1)(C)[C@H]2CC[C@@](O2)(CC)[C@H]2[C@H](C[C@@H](O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C[C@@]21C[C@H](O)[C@@H](C)[C@@H]([C@@H](C)[C@@H](OC)[C@H](C)C(O)=O)O2 GAOZTHIDHYLHMS-KEOBGNEYSA-N 0.000 claims description 5
- FCFNRCROJUBPLU-DNDCDFAISA-N valinomycin Chemical compound CC(C)[C@@H]1NC(=O)[C@H](C)OC(=O)[C@@H](C(C)C)NC(=O)[C@@H](C(C)C)OC(=O)[C@H](C(C)C)NC(=O)[C@H](C)OC(=O)[C@@H](C(C)C)NC(=O)[C@@H](C(C)C)OC(=O)[C@H](C(C)C)NC(=O)[C@H](C)OC(=O)[C@@H](C(C)C)NC(=O)[C@@H](C(C)C)OC1=O FCFNRCROJUBPLU-DNDCDFAISA-N 0.000 claims description 5
- VDXZNPDIRNWWCW-JFTDCZMZSA-N melittin Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(N)=O)CC1=CNC2=CC=CC=C12 VDXZNPDIRNWWCW-JFTDCZMZSA-N 0.000 description 20
- 108010036176 Melitten Proteins 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 6
- 108060003100 Magainin Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000000969 carrier Substances 0.000 description 4
- 238000002983 circular dichroism Methods 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 230000009881 electrostatic interaction Effects 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- KGQFJVRXCIGVSK-UHFFFAOYSA-N 3-(4-chlorophenyl)-1,2-dimethyldiaziridine Chemical compound CN1N(C)C1C1=CC=C(Cl)C=C1 KGQFJVRXCIGVSK-UHFFFAOYSA-N 0.000 description 2
- 238000009007 Diagnostic Kit Methods 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 102000004310 Ion Channels Human genes 0.000 description 2
- 108090000862 Ion Channels Proteins 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 238000010504 bond cleavage reaction Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001142 circular dichroism spectrum Methods 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 2
- 108010009551 Alamethicin Proteins 0.000 description 1
- 108700042778 Antimicrobial Peptides Proteins 0.000 description 1
- 102000044503 Antimicrobial Peptides Human genes 0.000 description 1
- 108010009685 Cholinergic Receptors Proteins 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 101100059320 Mus musculus Ccdc85b gene Proteins 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 102000034337 acetylcholine receptors Human genes 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- LGHSQOCGTJHDIL-UTXLBGCNSA-N alamethicin Chemical compound N([C@@H](C)C(=O)NC(C)(C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)NC(C)(C)C(=O)N[C@H](C(=O)NC(C)(C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NC(C)(C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NC(C)(C)C(=O)NC(C)(C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](CO)CC=1C=CC=CC=1)C(C)C)C(=O)C(C)(C)NC(=O)[C@@H]1CCCN1C(=O)C(C)(C)NC(C)=O LGHSQOCGTJHDIL-UTXLBGCNSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL12900399A IL129003A (he) | 1999-03-15 | 1999-03-15 | שיטה לגילוי קולורימטרי סלקטיבי עבור קטיונים בתמיסות מימיות |
| EP00909610A EP1161688B1 (en) | 1999-03-15 | 2000-03-14 | Colorimetric detection method |
| AU31889/00A AU3188900A (en) | 1999-03-15 | 2000-03-14 | Colorimetric detection method |
| DE60033640T DE60033640T2 (de) | 1999-03-15 | 2000-03-14 | Kolorimetrisches bestimmungsverfahren |
| PCT/IL2000/000158 WO2000055623A2 (en) | 1999-03-15 | 2000-03-14 | Colorimetric detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL12900399A IL129003A (he) | 1999-03-15 | 1999-03-15 | שיטה לגילוי קולורימטרי סלקטיבי עבור קטיונים בתמיסות מימיות |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL129003A0 IL129003A0 (en) | 2000-02-17 |
| IL129003A true IL129003A (he) | 2002-11-10 |
Family
ID=11072603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL12900399A IL129003A (he) | 1999-03-15 | 1999-03-15 | שיטה לגילוי קולורימטרי סלקטיבי עבור קטיונים בתמיסות מימיות |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1161688B1 (he) |
| AU (1) | AU3188900A (he) |
| DE (1) | DE60033640T2 (he) |
| IL (1) | IL129003A (he) |
| WO (1) | WO2000055623A2 (he) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL156869A0 (en) * | 2003-07-10 | 2004-09-27 | Univ Ben Gurion | Polydiacetylene-containing solid colorimetric detector, method for its preparation and uses thereof |
| US7794968B2 (en) | 2003-07-10 | 2010-09-14 | Ben-Gurion University Of The Negev Research And Development Authority | Polydiacetylene-containing solid colorimetric and/or fluorescent detector, method for its preparation and uses thereof |
| ATE494901T1 (de) * | 2004-11-09 | 2011-01-15 | Celtek Bioscience Llc | Zusammensetzung und verfahren zur erhöhung der apoptose in krebszellen |
| WO2006077586A2 (en) | 2005-01-19 | 2006-07-27 | Ben-Gurion University Of The Negev Research And Development Authority | Polydiacetylene-containing solid colorimetric and/or fluorescent detector, method for its preparation and uses thereof |
| US20210072213A1 (en) | 2018-01-03 | 2021-03-11 | Aarhus Universitet | Poly(diacetylene) sensor arrays for characterizing aqueous solutions |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0883690A4 (en) * | 1996-01-26 | 2002-07-31 | Univ California | PLASTIC FILM, ASSAY AND METHOD FOR DIRECTLY COLORIMETRIC DETECTION OF ANALYZES |
| WO1998039632A1 (en) * | 1997-03-03 | 1998-09-11 | The Regents Of The University Of California | Direct colorimetric detection of biocatalysts |
-
1999
- 1999-03-15 IL IL12900399A patent/IL129003A/he not_active IP Right Cessation
-
2000
- 2000-03-14 EP EP00909610A patent/EP1161688B1/en not_active Expired - Lifetime
- 2000-03-14 AU AU31889/00A patent/AU3188900A/en not_active Abandoned
- 2000-03-14 DE DE60033640T patent/DE60033640T2/de not_active Expired - Lifetime
- 2000-03-14 WO PCT/IL2000/000158 patent/WO2000055623A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1161688A2 (en) | 2001-12-12 |
| AU3188900A (en) | 2000-10-04 |
| DE60033640D1 (de) | 2007-04-12 |
| WO2000055623A3 (en) | 2001-03-22 |
| EP1161688B1 (en) | 2007-02-28 |
| IL129003A0 (en) | 2000-02-17 |
| DE60033640T2 (de) | 2007-11-08 |
| WO2000055623A2 (en) | 2000-09-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed | ||
| KB | Patent renewed | ||
| MM9K | Patent not in force due to non-payment of renewal fees |