IL124275A - שיטה לייצר רצפים של חומצות גרעין - Google Patents
שיטה לייצר רצפים של חומצות גרעיןInfo
- Publication number
- IL124275A IL124275A IL12427598A IL12427598A IL124275A IL 124275 A IL124275 A IL 124275A IL 12427598 A IL12427598 A IL 12427598A IL 12427598 A IL12427598 A IL 12427598A IL 124275 A IL124275 A IL 124275A
- Authority
- IL
- Israel
- Prior art keywords
- nucleic acid
- amplification
- probe
- detection
- reaction
- Prior art date
Links
- 150000007523 nucleic acids Chemical group 0.000 title claims abstract description 220
- 238000000034 method Methods 0.000 title claims abstract description 49
- 230000003321 amplification Effects 0.000 claims abstract description 157
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 157
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 137
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 137
- 238000012216 screening Methods 0.000 claims abstract description 12
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 11
- 239000002773 nucleotide Substances 0.000 claims abstract description 10
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 10
- 239000000539 dimer Substances 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 169
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 43
- 101150048508 RIC2 gene Proteins 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 126
- 238000001514 detection method Methods 0.000 description 105
- 238000003556 assay Methods 0.000 description 66
- 241000606153 Chlamydia trachomatis Species 0.000 description 64
- 238000012360 testing method Methods 0.000 description 62
- 229940038705 chlamydia trachomatis Drugs 0.000 description 60
- 239000003153 chemical reaction reagent Substances 0.000 description 59
- 102000004190 Enzymes Human genes 0.000 description 56
- 108090000790 Enzymes Proteins 0.000 description 56
- 239000000872 buffer Substances 0.000 description 36
- 239000013615 primer Substances 0.000 description 26
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 25
- 239000008188 pellet Substances 0.000 description 23
- 230000009977 dual effect Effects 0.000 description 21
- 108091093088 Amplicon Proteins 0.000 description 19
- 108091034117 Oligonucleotide Proteins 0.000 description 19
- 239000012530 fluid Substances 0.000 description 18
- 238000004925 denaturation Methods 0.000 description 16
- 230000036425 denaturation Effects 0.000 description 16
- 239000000203 mixture Substances 0.000 description 16
- 239000000758 substrate Substances 0.000 description 15
- 238000012545 processing Methods 0.000 description 14
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 13
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 13
- 238000011109 contamination Methods 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 12
- 239000013641 positive control Substances 0.000 description 12
- BCHIXGBGRHLSBE-UHFFFAOYSA-N (4-methyl-2-oxochromen-7-yl) dihydrogen phosphate Chemical compound C1=C(OP(O)(O)=O)C=CC2=C1OC(=O)C=C2C BCHIXGBGRHLSBE-UHFFFAOYSA-N 0.000 description 11
- 238000011901 isothermal amplification Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 9
- 238000010586 diagram Methods 0.000 description 9
- 230000002255 enzymatic effect Effects 0.000 description 9
- 238000010438 heat treatment Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 239000011541 reaction mixture Substances 0.000 description 9
- 230000035945 sensitivity Effects 0.000 description 9
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000002853 nucleic acid probe Substances 0.000 description 8
- 238000012546 transfer Methods 0.000 description 8
- 239000003155 DNA primer Substances 0.000 description 7
- 238000007792 addition Methods 0.000 description 7
- 208000015181 infectious disease Diseases 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 210000002700 urine Anatomy 0.000 description 6
- 102100034343 Integrase Human genes 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 238000000137 annealing Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000012898 sample dilution Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000013024 dilution buffer Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002751 oligonucleotide probe Substances 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
- 239000002987 primer (paints) Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000011535 reaction buffer Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000012795 verification Methods 0.000 description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 3
- 101100121035 Arabidopsis thaliana GCP2 gene Proteins 0.000 description 3
- 241000606161 Chlamydia Species 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- 101100478233 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SPR3 gene Proteins 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 238000011166 aliquoting Methods 0.000 description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000001351 cycling effect Effects 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000007834 ligase chain reaction Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000007826 nucleic acid assay Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 101710203526 Integrase Proteins 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- 229920004482 WACKER® Polymers 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012289 standard assay Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 208000019802 Sexually transmitted disease Diseases 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 235000021463 dry cake Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 244000000056 intracellular parasite Species 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000001935 peptisation Methods 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000006163 transport media Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical compound C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 description 1
- HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85017197A | 1997-05-02 | 1997-05-02 | |
| US09/065,914 US6558901B1 (en) | 1997-05-02 | 1998-04-24 | Nucleic acid assays |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL124275A true IL124275A (he) | 2002-03-10 |
Family
ID=26746169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL12427598A IL124275A (he) | 1997-05-02 | 1998-04-29 | שיטה לייצר רצפים של חומצות גרעין |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US7262008B2 (he) |
| AU (1) | AU755369B2 (he) |
| IL (1) | IL124275A (he) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6558901B1 (en) * | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
| CA2572178A1 (en) * | 2004-07-26 | 2006-03-16 | Nanosphere, Inc. | Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture |
| US7932081B2 (en) | 2005-03-10 | 2011-04-26 | Gen-Probe Incorporated | Signal measuring system for conducting real-time amplification assays |
| US20070141605A1 (en) * | 2005-11-21 | 2007-06-21 | Applera Corporation | Portable preparation, analysis, and detection apparatus for nucleic acid processing |
| US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
| US8718948B2 (en) | 2011-02-24 | 2014-05-06 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
| FR3067814A1 (fr) * | 2017-06-20 | 2018-12-21 | Biomerieux | Procede d'application, sur un support solide, d'au moins un partenaire de liaison a une molecule |
| US11492662B2 (en) | 2020-08-06 | 2022-11-08 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| EP4121557A4 (en) | 2020-08-06 | 2024-05-01 | Singular Genomics Systems, Inc. | Spatial sequencing |
Family Cites Families (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| US4457916A (en) * | 1982-08-31 | 1984-07-03 | Asahi Kasei Kogyo Kabushiki Kaisha | Method for stabilizing Tumor Necrosis Factor and a stable aqueous solution or powder containing the same |
| US4891319A (en) * | 1985-07-09 | 1990-01-02 | Quadrant Bioresources Limited | Protection of proteins and the like |
| US4762857A (en) * | 1986-05-30 | 1988-08-09 | E. I. Du Pont De Nemours And Company | Trehalose as stabilizer and tableting excipient |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| GB8715238D0 (en) | 1987-06-29 | 1987-08-05 | Quadrant Bioresources Ltd | Food process |
| WO1989000290A1 (en) | 1987-07-02 | 1989-01-12 | In Vitro Technologies, Inc. | Capillary device for immunoassay of multiple analytes |
| IE72468B1 (en) * | 1987-07-31 | 1997-04-09 | Univ Leland Stanford Junior | Selective amplification of target polynucleotide sequences |
| US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| GB8801338D0 (en) | 1988-01-21 | 1988-02-17 | Quadrant Bioresources Ltd | Preservation of viruses |
| US5229297A (en) * | 1989-02-03 | 1993-07-20 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
| GB8903593D0 (en) * | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| AU5269590A (en) * | 1989-03-10 | 1990-10-09 | Gene-Trak Systems | Immobilized oligonucleotide probes and uses therefor |
| US5217862A (en) * | 1989-05-24 | 1993-06-08 | Amoco Corporation | Method for the detection of Neisseria gonorrhoeae |
| WO1990015159A2 (en) | 1989-05-31 | 1990-12-13 | Gene-Trak Systems | Nucleic acid probes for the detection of chlamydia trachomatis |
| US5856088A (en) * | 1989-07-11 | 1999-01-05 | Gen-Probe Incorporated | Detection of human immunodeficiency virus type 1 |
| US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| US5122284A (en) * | 1990-06-04 | 1992-06-16 | Abaxis, Inc. | Apparatus and method for optically analyzing biological fluids |
| US5395521A (en) * | 1991-05-31 | 1995-03-07 | Board Of Regents, The University Of Texas System | Automated column equilibration, column loading, column washing and column elution |
| GB9114202D0 (en) | 1991-07-01 | 1991-08-21 | Quadrant Holdings Cambridge | Blood products |
| FR2679255B1 (fr) * | 1991-07-17 | 1993-10-22 | Bio Merieux | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
| US5521300A (en) * | 1991-08-13 | 1996-05-28 | Norval B. Galloway | Oligonucleotides complementary to mycobacterial nucleic acids |
| FR2688788B1 (fr) * | 1992-03-17 | 1994-05-13 | Bio Merieux | Composes hydrosolubles derives d'un homopolymere ou copolymere de l'anhydride maleique, et applications desdits composes au support de molecules biologiques. |
| EP0644950A4 (en) | 1992-04-09 | 1997-05-14 | Abbott Lab | ASSAY FOR THE DETECTION OF HIV ANTIGEN AND HIV ANTIBODIES. |
| US5587128A (en) * | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| JP4137996B2 (ja) * | 1992-05-06 | 2008-08-20 | ジェン−プローブ・インコーポレイテッド | 核酸配列増幅方法，組成物及びキット |
| WO1994002634A1 (en) * | 1992-07-24 | 1994-02-03 | University Of South Australia | Amplification and detection process |
| FR2694754B1 (fr) * | 1992-08-12 | 1994-09-16 | Bio Merieux | Fragments d'ADN de mycobactéries, amorces d'amplification, sondes d'hybridation, réactifs et procédé de détection de détection de mycobactéries. |
| WO1994005812A1 (en) * | 1992-09-02 | 1994-03-17 | The Scripps Research Institute | Coupled isothermal polynucleotide amplification and translation system |
| US5780273A (en) * | 1993-04-09 | 1998-07-14 | Amoco Corporation | Insertion elements and amplifiable nucleic acids |
| CA2119701A1 (en) | 1993-04-16 | 1994-10-17 | Colleen M. Nycz | Nucleic acid assay procedure |
| US5457027A (en) * | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
| US6709813B1 (en) * | 1993-05-14 | 2004-03-23 | Ortho-Clinical Diagnostics, Inc. | Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures |
| FR2709310B1 (fr) * | 1993-07-23 | 1995-09-29 | Bio Merieux | Fragment nucléotidique de l'ARN ribosomique 23S de mycobactéries, sondes et amorces dérivées, réactif et procédé de détection. |
| US6136529A (en) * | 1993-09-03 | 2000-10-24 | Gen-Probe Incorporated | Nucleic acid probes to Mycobacterium avium complex |
| FR2710075B1 (fr) * | 1993-09-15 | 1995-10-27 | Bio Merieux | Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal. |
| US5645801A (en) * | 1993-10-21 | 1997-07-08 | Abbott Laboratories | Device and method for amplifying and detecting target nucleic acids |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| KR100238833B1 (ko) * | 1994-03-10 | 2000-03-02 | 다니엘 엘. 캐시앙 | 이온성 세제에 의한 효소 개재 반응의 억제를 방지하는 방법 |
| US5487027A (en) * | 1994-05-18 | 1996-01-23 | Lord Corporation | Process and apparatus for providing an analog waveform synchronized with an input signal |
| CN1188171C (zh) | 1994-06-02 | 2005-02-09 | 廓德伦特控股剑桥有限公司 | 防止各种特质在再水化或熔化时聚集的方法以及由此获得的组合物 |
| US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
| US5589336A (en) * | 1994-11-10 | 1996-12-31 | New York Blood Center | Diagnostic method and kit for determining Kell blood group genotype |
| DE19503685C2 (de) * | 1995-01-30 | 2000-05-31 | Invitek Gmbh | Verfahren zur Herstellung komplexer multienzymatischer lagerstabiler Reaktionsgemische und deren Verwendung |
| US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| FR2730545B1 (fr) * | 1995-02-13 | 1997-03-28 | Bio Merieux | Vanne statique a congelation, et enceinte de traitement controlee par au moins une vanne |
| US5747252A (en) * | 1995-06-07 | 1998-05-05 | Gen-Probe Incorporated | Nucleic acid probes and amplification oligonucleotides for Neisseria species |
| US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US5762873A (en) * | 1996-02-21 | 1998-06-09 | Biomerieux Vitek, Inc. | Automatic sample testing machine |
| US5697409A (en) * | 1996-02-21 | 1997-12-16 | Biomerieux Vitek, Inc. | Diluting and pipetting stations for sample testing machine |
| CA2199213C (en) * | 1996-03-11 | 2007-04-17 | John Wesley Backus | Amplifying and detecting target nucleic acids using a post amplification incubation step |
| US5804384A (en) * | 1996-12-06 | 1998-09-08 | Vysis, Inc. | Devices and methods for detecting multiple analytes in samples |
| US6558901B1 (en) * | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
| US5786182A (en) * | 1997-05-02 | 1998-07-28 | Biomerieux Vitek, Inc. | Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use |
-
1998
- 1998-04-29 IL IL12427598A patent/IL124275A/he not_active IP Right Cessation
- 1998-05-01 AU AU63766/98A patent/AU755369B2/en not_active Expired
-
2002
- 2002-08-08 US US10/215,304 patent/US7262008B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU755369B2 (en) | 2002-12-12 |
| US7262008B2 (en) | 2007-08-28 |
| US20030077580A1 (en) | 2003-04-24 |
| AU6376698A (en) | 1998-11-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0875584B1 (en) | Nucleic acid assays | |
| US7329493B2 (en) | One-tube nested PCR for detecting Mycobacterium tuberculosis | |
| EP1921159A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| US8129110B2 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| US8207319B2 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| WO2007114518A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| US5508168A (en) | Methods and reagents for the detection of herpes simplex virus, treponema pallidum, and haemophilus ducreyi | |
| CA2121659C (en) | Sample processing method for mycobacteria | |
| WO2007114519A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| WO2007114511A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| WO2007114506A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| WO2007114509A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method | |
| JP4662578B2 (ja) | 抗生物質耐性微生物を同定するための方法およびキット | |
| JP2006525809A5 (he) | ||
| AU755369B2 (en) | Improved nucleic acid assays | |
| EP1726662A1 (en) | Instrument for detecting bacteria, method of detecting bacteria and kit for detecting bacteria | |
| AU769852B2 (en) | Improved nucleic acid assays | |
| EP3967772A1 (en) | Tools & methods usefool for detection of lactose intolerance and uses thereof | |
| MXPA98003500A (en) | Best nucleic acid experiments | |
| JP2010515452A (ja) | スタフィロコッカス・アウレウス検出用ｄｎａチップ | |
| WO2009087688A2 (en) | Method for detection of nucleic acid of mycobacterium and uses thereof | |
| EP3066210B1 (en) | Detection of streptococcus pneumoniae | |
| WO2007114516A1 (en) | Probe, probe set, probe-immobilized carrier, and genetic testing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed | ||
| MM9K | Patent not in force due to non-payment of renewal fees |