IL103749A - Method for production of transforming growth factor beta 2 - Google Patents
Method for production of transforming growth factor beta 2Info
- Publication number
- IL103749A IL103749A IL10374988A IL10374988A IL103749A IL 103749 A IL103749 A IL 103749A IL 10374988 A IL10374988 A IL 10374988A IL 10374988 A IL10374988 A IL 10374988A IL 103749 A IL103749 A IL 103749A
- Authority
- IL
- Israel
- Prior art keywords
- tgf
- protein
- nucleotide sequence
- activity
- growth factor
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
A method for production of transforming growth factor β
ONCOGEN
C: 87898
CLONING AND EXPRESSION OF
TRANSFORMING GROWTH FACTOR 02
TABLE OF CONTENTS
Page Introduction.... 3 Background of the Invention ;. 3 Summary of the Invention 5
3.1. Definitions 6 Brief Description of the Figures 8 Description of the Invention 10
.1. Isolation or Generation of The
TGF-^2 Coding Region 12
.2. Construction of Expression Vectors
Containing the TGF-/92 Coding
Sequence 17
.3. Identification of Transfectants or
Transformants Expressing the TGF-/92
Gene Product 20 Example: cDNA Cloning of TGF-/92
Precursor From PC-3 Cells 22
6.1. Materials and Methods 22
6.1.1. Growth of Cells and RNA
Extraction 22 6.1.2. cDNA Library Contruction
and Screening 22
6.1.3. Northern Blot Analysis. 24
6.2. Results 25 Example: cDNA Cloning of TGF- 2
Precursor From BSC-40 Cells 29
7.1. Material and Methods 30
7.1.1. Growth of Cells and RNA
Extraction 30
7.1.2. cDNA Library Construction
and Screening 30 esults 31 e: Expression of TGF-/S2 31 Materials and Methods 32
8.1.1. Cell Culture ...·.. 32
8.1.2. DNA Manipulations and Plasmid
Constructions 32
8.1.3. DNA Transfections 33
8.1.4. Selection of Methotrexate
Resistant Cells.... 34
8.1.5. Growth Inhibition Assay 34 Construction of TGF-^l/TGF-^2
Hybrid Precursor Gene for TGF-02
Expression 35 Expression of TGF-02 in CHO cells 36 t of. Microorganisms 37
L INTRODUCTION
The present application is a divisional application from Application No. 87877 and relates to the method for the cloning and expression of human transforming growth factor-Beta 2 and to eukaryiotic cells capable of such expression.
2. BACKGROUND OF THE INVENTION
Transforming growth factor-Beta (TGF-β) is a member of a recently described family of polypeptides that regulate cellular differentiation and proliferation. Other members of this family include Mullerian inhibitory substance (Cate et al., 1986, Cell 45 : 685-698 ) , the inhibins (Mason et al., 1985, ..Nature 318:659-663) and a protein predicted from a ., transcript of the decapentaplegic gene complex of Drosophila (Padgett et al., 1987, Nature 325:81-84).
Transforming growth factor-Beta (TGF-β) consists of two identical disulfide linked subunits having molecular weights of 13,000 (Assoian et al., 1983, J. Biol. Chera.
258:7155-7160; Frolik et al, 1983, Proc. Natl. Acad. Sci. USA 80:3676-3680; Frolik et al., 1984, J. Biol. Chem.
260:10995-11000). It has been purified from several tissue sources including placenta (Frolik et al., 1983, Nature
325:81-84), blood platelets (Childs et al., 1982, Proc.
Natl.- Acad. Sci. USA 79:5312-5316; Assoian- et al., 1983, J. Biol. Chem. 258:7155-7160) kidney (Roberts et al., 1983, Biochemistry 22:5692-5698), and demineralized bone (Seyedin et al., 1985, Proc. Natl. Acad. Sci. USA 82:119-123). In the presence of 10% serum and epidermal growth factor, TGF-β promotes the anchorage independent growth of normal rat kidney fibroblasts (Roberts et al., 1981, Proc. Natl. Acad. Sci. USA 78:5339-5343; Roberts et al. , 1982, Nature
295:417-419; Twardzik et al . , 1985, J. Cell. Biochem.
28:289-297); in the presence of 10% serum alone, it is able to induce colony formation of AKR-2B fibroblasts (Tucker et al., 1983, Cancer Res. 43:1518-1586). TGF-^ has also been shown to cause fetal rat muscle mesenchymal cells to
differentiate and produce cartilage specific macromolecules (Seyedin et al., 1986, J. Biol. Chem. 261:5693-5695).
In contrast to its effect on cell proliferation, TGF-0 purified from human platelets as well as a
functionally related protein isolated from African green monkey cells (BSC-1) has been shown to inhibit the growth of certain cells in culture (Tucker et al. , 1984, Science
226:705-707). TGF-Beta has also been shown to inhibit the growth of several human cancer cell lines (Roberts et al., 1985, Proc. Natl. Acad. Sci. USA 82:119-123). This
inhibitory/stimulatory effect of- TGF-β may depend on several factors including cell type and the physiological state of the cells (for review see Sporn et al., 1986, Science
233:532-534) .
cDNA clones coding for human (Derynck et al., 1985, Nature 316:701-705), mouse (Derynck et al., 1986, J. Biol. Chem. 261:4377-4379) and simian (Sharpies et al . , 1987, DNA 6:239-244) TGF-β have been isolated. DNA sequence analysis of these clones indicates that TGF-β is synthesized as a large precursor polypeptide, the carboxy terminus of which is cleaved to yield the mature TGF-3 monomer. Strong sequence homology has been found throughout the TGF-/9 precursor protein from all of the above sources.
Very recently a protein isolated from bovine
demineralized bone has been identified as being related to TGF-0 (Seyedin et al., 1987, J. Biol. Chem. 262: 1946-1949) . The protein has also been isolated from porcine platelets (Cheifetz et al., 1987, Cell 48:409-415), a human prostatic adenocarcinoma cell line, PC-3 (Ikeda et al., 1987,
Biochemistry 26:2406-2410), and a human glioblastoma cell line (Wrann et al., 1987, EMBO 6:1633-1636) . Partial amino acid sequence of this protein indicated that it was
homologous to TGF-β and has been termed TGF-^2. The human (Derynck et al., 1985, Nature 316:701-705), mouse (Derynck et al., 1986, J. Biol. Chem. 261:4377-4379) and simian
103749/3
(Sharpies et al., 1987, DNA6:239-244) TGF-β described previously has been termed TGF-βΙ.
Japanese Patent Application No. 61/219,395 (corresponding to
Israel Patent No. 78197) describes a method for the manufacture of TGF-(31 in a transformed eukaryotic cell line. This publication, however,
contains numerous errors in the disclosure which seriously call into
question whether TGF-βΙ can be produced on the basis of this publication, let alone a different protein such as TGF- 2.
3. SUMMARY OF THE INVENTION
The present invention relates to a method for the production of a protein having transforming growth factor-/?2 (TGF-/32) activity
comprising:
(a) culturing a eucaryotic cell containing a recombinant _ vector comprising a nucleotide sequence encoding a protein having transforming growth factor-jS2 activity, the protein being a mature TGF-j 2 , a TGF-02 precursor or a hybrid TGF-(Sl/TGF-j32 _ and comprising an amino acid sequence from about amino acid residue number 331 to 442 in the amino acid sequence depicted in Fig. la, the nucleotide sequence being under the control of a second nucleotide sequence that regulates gene expression so that a peptide or protein having TGF-jS2 activity is produced by the eucaryotic cell; and
(b) recovering the protein having TGF-/52 activity from the culture.
- 5 a- '"103749/1
In a specific embodiment, cDNA clones coding for human TGF- 2 precursor vera obtained from a cDNA library made from a tamoxifen treated human prostatic adenocarcinoma cell line, PC-3. The cDNA sequence of one such clone predicts that TGF- 2 is synthesized as a 442 amino acid polypeptide precursor from which zhe, mature 112 amino acid TGT-βΙ subunit is derived by proteolytic cleavage. This TGF-,52 precursor,' termed TGF-,32-442, shares a 41% homology with the precursor of TGF-,31. In another embodiment, cOtfA clones coding for simian TGF-,52 precursor were obtained from a cONA library made from an African green monkey kidney cell line, BCS-40. The cDNA sequence of one such clone, predicts that TGF-^2 is also synthesized as a 414 amino acid
polypeptide- precursor from which the mature 112 amino.'acid TGF-,32 subunit is derived by proteolytic cleavage. This TGF-^2 precursor, termed TGF-^2-414, has an amine acid sequence of 414 amino acid residues and is identical to the amino acid sequence of TGF-;52-442, except that it contains a single Asparagine residue instead of the 29 amino acid sequence from residue numbers 116 to 135 of the human
TGF-,52— 42 sequence.
Clones from the BSC-40 cDNA library which encode a simian TGF-52-442 precursor as well as clones from the human PC-3 cDNA library which encode a human TGF-,02-414 precursor have also been identified. The human and simian TGF-52-442 precursors appear to be -perfectly homologous at the amino
acid level, as do the human and simian TGF-/92-414
precursors.
The mature 112 amino acid monomers of TGF-91 and TGF-/92 show 71% homology.
Expression vectors containing the TGF-/S2 mature coding sequence joined in-phase to the TGF-,01 signal, and precursor sequences (EP-A-293,785) were constructed and used to transfect Chinese
Hamster Ovary cells (CHO cells). The resulting CHO transfectants produce and secrete mature, biologically active TGF-p2.
The scope of the invention is only as defined in the claims.
3.1. DEFINITION'S
The following 'terms as used herein whether in the singular or plural, shall have the meanings designated.
TGF-,02: A transforming growth factor-Beta2 of human or
simian origin comprising the amino acid
sequence substantially as depicted in FIG. la
from about amino acid residue number 331 to
about amino acid residue number 442.
TGF-02 A family of transforming growth factor-J3eta2 precursor: molecules of human or simian origin .comprising
an amino acid sequence substantially as
depicted in FIG. la from about amino acid
residue number 1 to about amino acid residue
number 442, or from about amino acid residue
number 1 to about amino acid residue number 442
where the amino acid sequence from amino acid
residue number 116 to amino acid residue number
144 is deleted and replaced by a single
Asparagine residue. The term shall mean a
TGF-beta 2 precursor designated TGF-,32-442 or
TGF-^2-414 , whether of human or simian origin.
Hybrid A novel transforming growth factor-beta
TGF-01/ precursor molecule comprising the amino acid TGF- 2 sequence substantially as depicted in FIG. lb precursor: from about amino acid residue number 1 to about amino acid number 390.
TGF-31 Simian-transforming growth factor-Betal
precursor: precursor and signal sequences substantially as depicted in FIG. lb from about amino acid
residue number 1 to about amino acid residue
number 278.
4. BRIEF DESCRIPTION OF THE FIGURES
FIG. la Nucleotide sequence of human
and deduced amino acid sequence. The 2597 bp insert of PC- 21 was subcloned into pEMBL (Dante et al., 1983, Nucleic 5 Acids Res. 11:1645-1654) and sequenced on both strands using the dideoxy chain-terminationi' method (Sanger et al., 1977, Proc. Natl. Acad. Sci. USA 74:5463-5467). The coding sequence is shown and the deduced amino acid sequence is presented directly above. The mature TGF-^2 sequence is Q boxed and the signal peptide is overlined. Potential glycosylation sites are indicated by asterisks. The arrow indicates the putative signal sequence cleavage site. The nucleotide sequence of simian TGF-^2-414 cDNA is identical to the human TGF-/92-442 cDNAr,sequence except that
nucleotides 346 through 432 (bracketed) are deleted and replaced by the sequence AAT, and except that several silent nucleotide changes occur elsewhere in the structure
(indicated by single letters directly below the changed nucleotide) . The deduced amino acid sequence for "simian TGF-02-414 precursor is identical to the human TGF-^2-442 precursor amino acid sequence except that Asparagine
replaces amino acid residues 116 through 144 in the human TGF-/92-442 structure. The nucleotide sequence of a human TGF-,fl2-414 cDNA has been sequenced through the region
. indicated by broken underlining and was found to be
perfectly homologous to the human TGF-^2-442 cDNA sequence except that nucleotides 346 through 432 are deleted and replaced by the sequence AAT.
FIG. lb Nucleotide sequence of hybrid TGF-/91/TGF- 2 j precursor DNA and deduced amino acid sequence. The coding sequence is shown and the deduced amino acid sequence is presented directly above. The mature TGF-/92 sequence is boxed and the precursor signal peptide is overlined.
Glycosylation sites are indicated by asterisks. The arrow . indicates the putative signal sequence cleavage site. The
TGF-^2 mature coding sequence depicted is of human origin. The simian TGF-^2 mature coding sequence is nearly identical to the human sequence: only 3 silent base changes occur and are indicated by single letters directly below the changed nucleotide. Details of the cDNA cloning of TGF- 2 and the construction of the hybrid TGF-/91/TGF-/92 gene are given in the text.
FIG. lc Schematic diagram of hybrid TGF-/91/TGF- 2 precursor gene.
FIG. Id Restriction endonuclease maps of pPC-14
(2.2kb) and pPC-21 (2.3 kb) . The boxed regions indicate coding sequences for TGF-/32 monomer. The ATG denotes the initiating methionine codon. The distance between the ATG and Kpnl site- in ρΡ021 (2.34 kb) - is .-approximately 420 bp. The darkened area indicates the position of the 84-bp insertion in pPC-21 (2.3 kb) .
FIG. le Partial DNA sequence analysis of pPC-14 (2.2 kb) . A synthetic oligonucleotide 5 ' -AGGAGCGACGAAGAGTACTA-3 ' which hybridized approximately 140 bp upstream from the Kpnl site within the insert in pPC-21 (2.3 kb) was used to prime DNA sequencing reactions. In this region, the sequence of pPC-14 (2.2 kb) (upper line) is identical to pPC-21 (2.3 kb) up to nucleotides coding for Asn-116. The 84-bp insertion within the Asn-116 codon of pPC-14 (2.2 kb) which was found in pPC-21 (2.3 kb) is shown. The Kpnl site within the insert is denoted.
FIG. 2 Homologies of human TGF-/91 and TGF-^2-442 precursor sequences, a) Primary sequence homology:
identical residues are boxed. Asterisks refer to potential glycosylation sites in TGF-^2. The potential signal
sequence cleavage site and the cleavage site of the mature polypeptide are indicated, b) Dot matrix comparison using Gene Pro software. Each dot locates a point where 5 out of 10 amino acids are identical. Diagonal lines indicate regions of homology.
FIG. 3 Northern blot analysis of BSC-40 and PC-3
polyadenylated RNA. Polyadenylated RNA was isolated from
BSC-40 and PC-3 cells, fractionated on an agarose- formaldehyde gel, transferred to Hybond-N filters and
32
hybridized to [ P] -labelled TGF-/32 specific probe, pPC-21
32
(Panel A) or a mixture of [ P] -labelled TGF-02 and TGF-βΐ (Sharpies et al.,1987) specific probes (Panel B) as
described in Materials and Methods. Lane 1, BSC-40
polyadenylated RNA (5 micrograms); lane 2, PC-3
polyadenylated RNA (5 micrograms) .
FIG. 4 Northern blot analysis of polyadenylated RNA from different sources. Polyadenylated RNA was isolated from MCF-7 (human mammary carcinoma) , SK-MEL 28 (human
melanoma) , KB (nasopharangeal carcinoma) and HBL-100 (human mammary epithelial) cells and analyzed by Northern blot
hybridization to a TGF-^2 specific probe (pPC-21) as
described 'in Materials and Methods. Each lane contains 5 microgram's of polyadenylated RNA from SK-MEL 28 (lane 1) ,
MCF-7 (lane 2), HBL-100 (lane-3) or KB (lane 4) cells.
FIG. 5 Bioactivity Assay of Recombinant TGF- 2. 1β9
12.5, clone 36 cells were grown to confluency in 100 mm
tissue culture dishes. Cells were washed 3X with serum-free media and incubated for 24 hours in 5 ml of serum-free
media. Media was collected, dialyzed against 0.2M acetic acid, and assayed for inhibition of DNA synthesis in CCL64 cells as described (Gentry et al., 1987, Mol. Cell. Biol.
7:3418). In this assay 3.3 pg of purified natural TGF-βΙ stan dard gave 50% inhibition; the specific activity. of purified natural TGF- 2 was calculated to be about half that of TGF-βΙ.
FIG. 6 Western Blot Analysis of Recombinant Proteins
Secreted by 1/39, 12.5, clone 36. Acid dialyzed serum-free conditioned media from 1β9 , 12.5, clone 36 cells was
fractionated by SDS-polyacrylamide gel electrophoresis and analyzed by Western blotting with anti serum made against
I
the synthetic peptide NH2-YNTINPEASASPC-C00H as described (Gentry et al., 1987, Mol. Cell. Biol. 7:3418) .
. DESCRIPTION OF THE INVENTION
The present invention relates to the production of a biologically active, mature form of TGF-02 from a TGF-0 precursor gene coding sequence and its product. The mature biologically active TGF-02 may be produced by the cloning and expression of the full-length nucleotide coding sequence 0 of the TGF-/92 precursor or its functional equivalent in a host cell which processes the precursor correctly so that a mature TGF- 2 is produced having a biological activity that is virtually indistinguishable from that of authentic natural TGF-/92. Functional equivalents- ..of the full length 5 nucleotide coding sequence of the TGF-02 precursor include any DNA sequence which, when expressed inside an appropriate host cell, is capable of directing the synthesis, processing and export of mature TGF-02. In this regard, hybrid
precursor coding sequences including, for example, the TGF-Q βΐ precursor sequence joined in-frame to the TGF-^2 mature sequence, may be constructed and used to produce
biologically active TGF-^2.
The method of the invention may be divided into the following stages solely for the purposes of description: (a) 5 isolation or generation of the coding sequence for a
precursor form of TGF-02 (b) construction of an expression vector which will direct the expression of a TGF-32 coding sequence; (c) transfection of appropriate host cells which are capable of replicating and expressing the gene and
0 processing the gene product to produce the mature,
biologically active form of TGF-/92 ; and (d) identification and purification of the mature, biologically active TGF-^2. Once a transfectant is identified that expresses high levels of bioactive, mature TGF-02 , the practice of the invention
involves the expansion of that clone and isolation of the gene product expressed.
.The method of the invention is demonstrated herein, by way of examples in which cDNAs of the TGF-92 precursor coding region were prepared, cloned, sequenced, and utilized to construct expression vectors which direct the high-level expression of TGF-^2 in CHO cells. In a specific
embodiment, the complete amino acid sequence of the mature form of human TGF- 2 has been determined and shows an overall homology of 71% with TGF-^1. Using synthetic oligonucleotide probes we have identified clones from a PC-3 cDNA library coding for TGF-02. DNA sequence analysis of one of these clones revealed that TGF-^2, like TGF-/91, is synthesized as a larger precursors-protein, the carboxy terminus of which is cleaved to yield the mature TGF-02 monomer. While there is a 71% homology between TGF-/91 and TGF-/92 throughout the mature portions of these molecules, only a maximum of 31% homology exists within the rest of. the precursor, suggesting that the amino terminal regions of TGF-/91 and TGF-02 may be functionally distinct.
In a specific embodiment of the invention, expression of a novel TGF-01/TGF-02 hybrid gene in CHO cells is used to produce large amounts of biologically active TGF-/92.
The various aspects of the method of the invention are described in more detail in the subsections below and in the examples that follow.
.1. ISOLATION OR GENERATION OF THE
TGF-BETA 2 CODING REGION
The nucleotide coding sequence for TGF-^2 is depicted in FIG. la. In the practice of the method of the invention, the nucleotide sequence depicted therein, or fragments or functional equivalents thereof, may be used to generate the recombinant molecules which will direct the expression of the TGF- 2 product in appropriate host cells. In a specific
embodiment, a TGF-/91/TGF-/92 hybrid gene (FIG. lb) was constructed and used to transfect CHO cells. Transfectants producing as much as 500 μq mature, biologically active TGF-92 per ml culture medium were isolated.
Due to the degeneracy of the nucleotide coding sequences, other DNA sequences which encode substantially the same amino acid sequences as depicted in FIG. la and FIG. lb may be used in the practice of the present invention for the cloning and expression of TGF-^2. Such alterations include deletions, additions or substitutions of different nucleotide residues resulting in a sequence that encodes the same or a functionally equivalent gene product. The gene product may contain deletions, additions or substitutions of amino acid residues within the sequenced' which result in a silent change thus producing a bioactive product. Such amino acid substitions may be made on the basis of
similarity in polarity, charge, solubility, hydrophobicity , hydrophilicity and/or the amphipathic nature of the residues involved. For example, negatively charged amino acids include aspartic acid and glutamic acid; positively charged amino acids include lysine and arginine; amino acids with uncharged polar head groups or nonpolar head groups having similar hydrophilicity values include the following:
leucine, isoleucine, valine; glycine, alanine; asparagine, glutamine; serine, threonine; phenylalanine, tyrosine.
The nucleotide coding sequence for TGF-^2 may be obtained from cell sources that produce TGF-^2 like
activity. The coding sequence may be obtained by cDNA cloning of RNA isolated and purified from such cellular sources or by genomic cloning. Either cDNA or genomic libraries of clones may be prepared from the DNA fragments generated using techniques well known in the art including but not limited to the use of restriction enzymes. The fragments which encode TGF-^2 may be identified by screening such libraries with a nucleotide probe that is substantially
complementary to any portion of the sequence depicted in FIG. la. Full length clones, i.e. , those containing the entire coding region for the TGF^02 precursor may be selected for expression.
In an alternate embodiment of the invention, the coding sequence of FIG. la could? be synthesized in whole or part, using chemical methods well known in the art. See, for example, Caruthers et al., 1980, Nuc. Acids Res. Symp. Ser. 7:215-223; Crea and Horn, 1980, Nuc. Acids. Res.
9(10):2331; Matteucci and Carruthers, 1980, Tetrahedron Letters 21:719 and Chow and Kempe, 1981, Nuc. Acids. Res. 9 (12 ): 2807-2817. Alternatively, the protein could be produced' using chemical methods to synthesize the amino acid sequence depicted in FIG. la I :whole or in part. For example, peptides can be synthesized by solid phase
techniques on a Beckman 990 instrument, and cleaved from the resin as previously described (Gentry, L.E., et al., 1983, J. Biol. Chem. 258:11219-11228; Gentry, L.E. and Lawton, A., 1986, Virology 152:421-431). Purification can be
accomplished by preparative high performance liquid
chromatography. The composition of the peptides was
confirmed by amino acid analysis.
In a specific embodiment, described in the examples herein, the TGF- 2 coding sequence was obtained by cDNA cloning of human TGF-92 precursor coding sequences derived from polyadenylated RNA isolated from tamoxifen-treated human prostatic adenocarcinoma cell line, PC-3, previously shown to produce TGF- 2. The entire coding region of one cD A clone was sequenced and compared to the published sequence of human TGF-91 (see FIG. 2) .
DNA sequence analysis of TGF-02 cDNA clones indicates that TGF-^2, like TGF-01, is synthesized as a large
precursor protein, the carboxy terminus of which is cleaved to yield the mature 112 amino acid TGF-/92 monomer. TGF-,02 has been shown to have a molecular weight of 24,000 composed
of two disulfide-linked 13,000 daltorr subunits (Ikeda et al., 1987, Biochemistry 26:2406-2410; Cheifetz et al., 1987, Cell 48:409-415). Therefore, the production of mature TGF- 2 requires proper proteolytic cleavage as well as the formation of intra- and inter-molecular disulfide bonds. An amino terminal hydrophobic leader sequence (residue 3-19) is present in the precursor and may be responsible for
directing the protein out of the cell. The mature TGF-02 may still be associated with the remaining portion of the precursor during this process.
TGF-02 shows 71% homology with TGF-,91 throughout the mature portion of the precursor, implying a functional similarity which is supported by experimental evidence
(Seyedin et al., 1987, J. Biol. Chem. .2:62: 1946-1949;
Cheifetz et al., 1987, Cell 48:409-415). The amino portion of the precursor region of TGF-/91 from human, rodent and simian sources (Derynck et al., 1985, Nature 316:701-705; Derynck et al., 1986, J. Biol. Chem. 261:4377-4379; Sharpies et al., 1987, DNA 6:239-244) is highly conserved and
suggests that this part of the molecule may have an
important biological function. In contrast, there is no more than 31% homology between the N-terminal precursor regions of TGF-01 and TGF- 2. After cleavage of the
putative signal peptide, the TGF-02 precursor would also contain more amino acids than TGF-01 precursor. The primary structural differences within the amino terminal region of the TGF-^1 and TGF-^2 precursor proteins may reflect
functional differences. However, significant homologous regions within the precursors are found in isolated blocks suggesting the conservation of important functional domains even within the N-terminal precursor region.
Northern blot analysis revealed two major size classes of TGF- 2-specific mRNA of 4.1 and 6.5kb in BSC-40 cells.
Tamoxifen-treated PC 3 cells contain three TGF- 2
transcripts of 4.1kb, 5.1kb, and 6.5kb. These different-
sized messages could be the result, of differential RNA splicing, polyadenylation, or both as has been described for other genes (Helfman et al . , 1986, Mol. Cell. Biol. 6:3582- 3595; Sayre et al., 1987, Proc. Natl. Acad. Sci. USA
84:2941-2945). Preliminary analysis of another TGF-/92 cDNA clone shows that it¾ contains a 3 ' ^untranslated region approximately lkb larger than that of pPC-21 and pPC-14 and contains a different polyadenylation site suggesting that alternative polyadenylation is one factor responsible for the generation of multiple TGF-/S2 m NAs observed on Northern blots.
BSC-40 cells contain comparable levels of TGF-^31 and TGF- 2-specific transcripts:' tamoxifen-treated PC-3 cells contain more TGF-01 mRNA than .TGF-02.. (FIG. 3B) . The latter result is unexpected since these cells produce more TGF-92 protein than TGF-01 (Ikeda et al, 1987, Biochemistry
26:2406-2410) and suggests a post transcriptional level of regulation regarding the synthesis of this growth modulator. Experiments aimed at understanding transcriptional and translational control mechanisms which lead to the
production of TGF-/91 and TGF-^2 are in progress. Production of adequate amounts of TGF-^2 by recombinant DNA techniques, as has been done for TGF-01, should aid further in designing experiments to explore the different effects of this
protein.
In another embodiment, the TGF-^2 coding sequence was obtained by cDNA cloning of simian TGF-/92 precursor coding sequences derived from polyadenylated RNA isolated from an African green monkey cell line, BSC-40. The entire coding region of one cDNA clone was sequenced. The human and simian TGF- 2 precursors appear to have identical amino acid sequences, and their nucleotide sequences are nearly
identical.
.2. CONSTRUCTION OF EXPRESSION VECTORS
CONTAINING THE TGF-BETA 2 CODING SEQUENCE
In order to express a biologically active, mature form of TGF-/92, an expression vector/host system should be chosen which provides not only for high levels of transcription and translation but for the correct processing of the gene product. This is especially important when employing the entire coding sequence of a TGF-^2 precursor in the
expression constructs because the mature form of TGF-92 appears to be derived from the precursor product via
cellular processing events. In addition, an expression/host cell system which provides for secretion of the product may be selected.
In particular, it appears that the, mature TGF-02 , a disulfide-linked homodimer of 112 amino acids per subunit may be formed by cellular processing involving proteolytic cleavage between the Arg-Ala amino acids of the precursor (residue numbers 330 and 331 in FIG. la) . In addition, the TGF- 2 precursor contains three potential N-glycosylation sites not found in the mature form; the proper glycosylation of the precursor may be important to the cellular synthesis and release or secretion of the mature molecule. Moreover, the mature form of TGF- 2 comprises a disulfide-linked dimer involving nine cysteine residues per subunit. Some of these are involved in interchain and others in intrachain
disulfide bonds which affect the tertiary structure and configuration of the mature molecule, and, as a result, its biological activity. Thus, the ability of a host cell used in the expression system to correctly express and process the TGF-92 gene product is important to the production of a biologically active, mature TGF-02.
A variety of animal host/expression vector systems (i.e. , vectors which contain the necessary elements for directing the replication, transcription and translation of the TGF-02 coding sequence in an appropriate host cell) may
be utilized equally well by the skilled artisan. These include, but are not limited to, virus expression
vector/mammalian host cell systems (e.g. , cytomegalovirus, vaccinia virus, adenovirus, and the like) ; insect virus expression vector/insect cell systems (e.g. , baculovirus) ; or nonviral promoter expression systems derived from the genomes of mammalian cells (e.g. , the mouse metallothionine promoter) .
The expression elements of these vectors vary in their strength and specificities. Depending on the host/vector system utilized, any one of a number of suitable
transcription and translation elements may be used. For instance, when cloning in mammalian cell systems, promoters isolated from the genome of mammalian-' cells , (e.g. mouse metallothionien promoter) or from viruses that grow in these cells, (e.g. vaccinia virus 7.5K promoter) may be used.
Promoters produced by recombinant DNA or synthetic
techniques may also be used to provide for transcription of the inserted sequences.
Specific initiation signals are also required for sufficient translation of inserted protein coding sequences. These signals include the ATG initiation codon and adjacent sequences. In cases where the entire TGF- 2 gene including its own initiation codon and adjacent sequences are inserted into the appropriate expression vectors, no additional translational control signals may be needed. However, in cases where only a portion of the coding sequence is
inserted, exogenous translational control signals, including the ATG initiation codon, must be provided. Furthermore, the initiation codon must be in phase with the reading frame of the TGF-^2 coding sequences to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of
origins, both natural and synthetic. The efficiency of
expression may be enhanced by the inclusion of transcription attenuation sequences, enhancer elements, etc.
Any of the methods previously described for the insertion of DNA fragments into a vector may be used to construct expression vectors containing the TGF-/9 gene and appropriate transcriptional/translational control signals. These methods may include in vitro recombinant DNA
techniques, synthetic techniques and in vivo recombinations (genetic recombination) .
In cases where an adenovirus is used as an expression vector, the TGF-^2 coding sequence may be ligated to an adenovirus transcription/translation control complex, e.g., the late promoter and tripartite leader sequence. This chimeric gene may then be inserted in the? adenovirus genome by in vitro or in vivo recombination. Insertion in a nonessential region of the viral genome (e.g. , region El or E3) will result in a recombinant virus that is viable and capable of expressing TGF- 2 in infected hosts. Similarly, the vaccinia 7.5K promoter may be used.
An alternative expression system which could be used to express TGF-/92 is an insect system. In one such system, Autographa californica nuclear polyhedrosis virus (AcNPV) is used as a vector to express foreign genes. The virus grows in Spodoptera frugiperda cells. The TGF- 2 coding sequence may be cloned into non-essential regions (for example the polyhedrin gene) of the virus and placed under control of an AcNPV promoter (for example the polyhedrin promoter) .
Successful insertion of the TGF-02 coding sequence will result in inactivation of the polyhedrin gene and production of non-occluded recombinant virus (i.e., virus lacking the proteinaceous coat coded for by the polyhedrin gene) . These recombinant viruses are then used to infect Spodoptera frugiperda cells in which the inserted gene is expressed.
In addition, a host cell strain may be chosen which modulates the expression of the inserted sequences, or
modifies and processes the gene product in the specific fashion desired. Expression from certain promoters can be elevated in the presence of certain inducers, (e.g. zinc and cadmium ions for metallothionein promoters) . Therefore, expression of the genetically engineered TGF-/92 may be controlled. This is important if the protein product of the cloned foreign gene is lethal to host cells. Furthermore, modifications (e.g. glycosylation) and processing (e.g., cleavage) of protein products are important for the function of the protein. Different host cells have characteristic and specific mechanisms for the post-translational
processing and modificatin of proteins. Appropriate cell lines or host systems can be chosen to ensure the correct modification and processing of the foreign protein
expressed.
.3. IDENTIFICATION OF TRANSFECTANTS OR TRANSFOR- MANTS EXPRESSING THE 2 TGF-BETA GENE PRODUCT
The host cells which contain the recombinant" TGF-,92 coding sequence and which express the biologically active, mature product may be identified by at least four general approaches: (a) DNA-DNA hybridization; (b) the presence or absence of "marker" gene functions; (c) assessing the level of transcription as measured by the expression of TGF-^2 mRNA transcripts in the host cell; and (d) detection of the mature gene product as measured by immunoassay and,
ultimately, by its biological activity.
In the first approach, the presence of the TGF-02 coding sequence inserted in the expression vector can be detected by DNA-DNA hybridization using probes comprising nucleotide sequences that are homologous to the TGF-92 coding sequence substantially as shown in FIG. la, or portions or derivatives thereof.
In the second approach, the recombinant expression vector/host system can be identified and selected based upon
the presence or absence of certain "marker" gene functions (e.g. , thymidine kinase activity, resistance to antibiotics, resistance to methotrexate, transformation phenotype, occlusion body formation in baculovirus, etc.). For
example, if the TGF-^2 coding sequence is inserted within a marker gene sequence of the vector, recombinants containing the TGF-92 coding sequence can be identified by the absence of the marker gene function. Alternatively, a marker gene can be placed in tandem with the TGF-^2 sequence under the control of the same or different promoter used to control the expression of the TGF-^2 coding sequence. Expression of the marker in response to induction or selection indicates expression of the TGF-/92 coding sequence.
In the third approach, transcriptional activity for the TGF-beta 2 coding region can be assessed by
hybridization assays. For example, polyadenylated R A can be isolated and analyzed by Northern blot using a probe homologous to the TGF-beta 2 coding sequence or particular portions thereof. Alternatively, total nucleic acids of the host cell may be extracted and assayed for hybridization to such probes.
In the fourth approach, the expression of the mature protein product can be assessed immunologically, for example by Western blots, immunoassays such as radioimmuno-precipitation, enzyme-linked immunoassays and the like. The ultimate test of the, success of the expression system, however, involves the detection of the biologically active TGF-/92 gene product. Where the host cell secretes the gene product the cell free media obtained from the cultured transfectant host cell may be assayed for TGF-/32 activity. Where the gene product is not secreted, cell lysates may be assayed for such activity. In either case, biological assays such as the growth inhibition assay described herein or the stimulation of anchorage independent growth in target cells (Twardzik and Sherwin, 1985, J. Cell. Biochem.
28:289-297; Delarco and Todaro, 1978, Proc. Natl. Acad. Sci. U.S.A. 75:4001-4005) or the like may be used.
Once a clone that produces high levels of biologically active, mature TGF-32 is identified, the clone may be expanded and the TGF-02 may be purified using techniques well known in the art. Such methods include immunoaffinity purification, chromatographic methods including high
performance liquid chromatography, and the like.
EXAMPLE: cDNA CLONING OF TGF
PRECURSOR FROM PC-3 CELLS
The following examples describe the cDNA cloning of TGF-/92 precursor coding sequences from the human prostatic adenocarcinoma cell line, PC-3, from. which TGF-beta-2 was previously isolated.
6.1. MATERIALS AND METHODS
The following procedures were used to clone cDNAs encoding human TGF-beta 2 precursor.
6.1.1. GROWTH OF CELLS AND RNA EXTRACTION.
The human prostatic adenocarcinoma cell line, PC-3, was grown in tamoxifen-supplemented medium as described (Ikeda et al., 1987, Biochemistry 26:2406-2410). MCF-7 cells were grown in Dulbecco's modified Eagle's medium containing 10% fetal calf serum and 6 units/ml insulin. All other cell lines were grown in the same medium without insulin. Polyadenylated RNA was isolated by oligo[dT]-cellulose chromatography as described (Purchio and Fareed, 1979, J. Virol. 29:763-769) .
6.1.2. CDNA LIBRARY CONSTRUCTION AND SCREENING
Double-stranded cDNA was synthesized from
polyadenylated RNA isolated from PC-3 cells treated with tamoxifen for 24 hours as described (Maniatis et al., 1982,
in Molecular Cloning: A Laboratory Manual,- Cold Spring
Harbor Press, Cold Spring' Harbor, New York) . cDNA fractions greater than 1000 base pairs were cloned into lambda gtlO as described (Webb et al., 1987, DNA 6:71-79). The library was
32
first screened in duplicate with a [ P] -labeled 24-fold degenerate probe complimentary to DNA encoding amino acids WKWIHEP (probe 1) which are conserved between TGF-31 and TGF-^2 :
[ 5 ' -GGTTCGTGTATCCATTTCCA-3 ' ]
C A G C A
Positive clones were then screened with, a second 128-fold degenerate probe complimentary to DNA encoding amino acids
CFRNVQD (probe 2); five out of these seven amino acids are specific for TGF-Beta 2:
[ 5 ' TCTTGAACGTTTCTGAAGCA-3 ' ]
C C A C A A G T
Hybridization was performed at 42 "C in 6XSSC, 5X Denhart's solution, 0.15 mM pyrophosphate, 100 micrograms/ml denatured calf thymus DNA, 100 micrograms/ml yeast tRNA and 1 mM EDTA. (Maniatis et al., 1982, in Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor, New York). Filters were washed at 42°C in 2XSSC, 0.1% NaDodS04, four times for 30 rain. Several cDNA clones were isolated which hybridized to both probes and were subcloned into pEMBL (Dante et al., 1983, Nucleic Acids Res. 11:1645-1654). One clone (pPC-21) containing a 2.6kb insert was sequenced on both strands by the dideoxy chain-termination method (Sanger et al., 1977, Proc. Natl. Acad. Sci. USA 74:5463-5467) using various restriction and exonuclease III deletion fragments combined with specific oligonucleotide priming (Henikoff, 1984, Gene 28:351-359). Another clone (pPC-14) containing a 2.2 kb insert was partially sequenced. Dot
matrix analysis was performed on an IBM ATPC using Gene Pro software from Riverside Scientific Enterprises (Seattle, WA) .
6.1.3. NORTHERN BLOT ANALYSIS
Polyadenylated -RNA was fractionated on a 1%
agaroseformaldehyde gel (Lehrach et al., 1977, Biochemistry
16:4743-4751), transferred to a nylon membrane (Hybond,
32
Amersham) , and hybridized to [ P] -labeled probe.
Hybridization was carried out at 42 'C in 50% formamide containing 0.9 M NaCl, 50 mM sodium phosphate (pH 7.0), 5 mM EDTA, 0.1% NaDodS04, 4X Denhardt's solution, 0.4 mg/ml of yeast tRNA, and 0.25 mg/ml of denatured calf thymus DNA. Filters were washed at 65" C in' 0.25X' SSC, 0.1% NaDodS04, dried, and exposed to Cronex-4 X-ray film (DuPont) with the aid of Lightening Plus intensifier screens (DuPont) .
6.2. RESULTS
A cDNA library was constructed using polyadenylated RNA isolated from tamoxifen treated PC-3 cells. Earlier observations indicated that tamoxifen treatment resulted in a 2- to 5-fold increase in the secretion of TGF-02 (Ikeda et al., 1987, Biochemistry 26:2406-2410). The library was screened with probes 1 and 2 as described above. Five clones were obtained which hybridized to both probes: one clone, pPC-21, containing a 2.6kb insert, was chosen for sequencing. Another clone, pPC-14, containing a 2.2 kb insert, was partially sequenced. The DNA and deduced amino acid sequences are shown in figure 1.
pPC-21 contains a single open reading frame coding for a deduced polypeptide of 442 amino acids; the 112 carboxy terminal amino acids comprise the mature TGF-02 monomer (boxed in FIG. la) . The first methionine encoded by the open reading frame is immediately followed by a stretch of hydrophobic and uncharged amino acids (overlined in FIG. la)
characteristic of a signal .peptide . Neither the nucleotide sequence coding for this methionine nor those coding for the next two methionines present in the open reading frame are homologous to the consensus sequence for the initiating
methionine sequence (Kozak, 1986, Cell 44:283-292). Because translation usually initiates with the first methionine in an open reading frame and because regions homologous to TGF- 1, as discussed below, occur upstream of the second methionine, the first methionine has been tentatively
Q assigned as the site of translation initiation. It appears then, that. TGF-^2, like TGF-01, is expressed as part of a much larger secreted precursor. The pPC-21 clone contains 467 bp upstream of the putative initiating methionine and a 3' untranslated region
including a 5 P°ly [A] track, fifteen bases upstream of which is located a polyadenylation signal sequence (Proudfoot and Brownlee, 1976, Nature 263:211-214).
The nucleotide sequence homology within the coding regions of the TGF-/91 and TGF-02 pPC-21 cDNA clone was
Q determined to be 53%. The regions coding for the mature proteins have 57% homology while the upstream precursor regions have 48% homology. After optimal- alignment of the two sequences, several nucleotide insertions were noted in the TGF-02 precursor region, one of which extended for 75 5 nucleotides. Whether these insertions are due to the presence of extra exons in TGF-^2 is unknown. No
significant homology was detected between the DNA sequences in the non-coding regions of the two clones. In fact, while TGF-/91 had extended G-C rich non-coding regions, TGF-/92 had g extensive A-T rich non-coding regions. Both cDNA clones contain repeating structural motifs in the 3' noncoding region with the repeats in TGF-01 consisting of (purine) CCCC (Sharpies et al., 1987, DNA 6:239-244) and in TGF-/92 of ATG or A(pyrimidine) (purine) .
Restriction mapping of many clones revealed that one clone, pPC-14, lacked a Kpnl restriction site located in the amino portion of TGF-,02 coding sequence. Restriction maps of pPC-14 and pPC-21 are shown in FIG. Id. pPC-14 was sequenced over a strech of about 100 nucleotides
corresponding to this-region of the molecule by specifically priming with a 20-mer oligonucleotide complementary to nucleotides 277 to 296 in FIG. la. The results show that the pPC-14 clone contains an 87 nucleotide deletion
(nucleotide positions 346 to 432 in FIG. la; see also FIG. le) that accounts for the missing Kpnl site and which is replaced by the sequence AAT, the codon for Asparagine. The results suggest that the pPC-14 clone encodes a shorter TGF-^2 precursor of 41 amino acids ^differing from the sequence encoded by pPC-21 only in that amino acid residues 116 through 144 are deleted and replaced by a single-Asparagine residue.
Although the entire coding region of pPC-14 was not determined, it is probably in perfect agreement with the pPC-21 coding sequence since, except for the Kpnl site, restriction maps of the two clones overlap perfectly (FIG. Id) . Furthermore; a simian clone encoding a 414 amino acid TGF-β precursor containing the same 29 amino acid deletion and replacement has been identified, as described in example Section 7, infra. This simian clone has a coding sequence which is nearly identical to that of the human pPC-21 clone in the regions 5' and 3' to the deletion.
Figure 2A shows the deduced protein sequence of human TGF-^1 (Derynck et al., 1985, Nature 316:701-705) compared to that of human TGF-/92-442. It was determined that ΎGΈ-β2 is 71% homologous with human TGF-^1 throughout the mature portion of the molecule as reported previously (Marquardt et al., 1987, J. Biol. Chem. , in press). The amino portion of the precursor upstream of the mature molecule shows a 31% homology between TGF-/91 and TGF-/92-442. The dot matrix
homology comparison shown in figure 2B reveals that
significant homology exists in several specific areas of the proteins. Comparison of the N-terminal amino acid sequences in the putative signal peptide region reveals no significant homology.
In TGF-/92, the signal sequence cleavage site is predicted to be after amino acid 20 (serine) and after amino acid 29 (glycine) in TGF-/91 (Von Heijne, 1983, Eur. J.
Biochem. 133:17-21). This cleavage site directly precedes the first block of homology between TGF-/91 and TGF-^2 which extends for 34 amino acids downstream. After removal of the signal sequences, the TGF-^1 and TGF- 2 precursors would share identical N-termini over the first four amino acids, including the cysteine at. position 4. Fourteen amino acids downstream of this putative N-terminus, 19 out of the next 21 amino acids are conserved between TGF-βΙ and TGF-92 , a homology block larger than any seen even in the C-terminal region containing the mature TGF-β protein. Several more blocks of strong homology exist within the region upstream of the mature protein as seen in figure 2A and 2B. These domains are separated by long stretches of non homologous amino acids.
The TGF-/92 precursor has three potential N-glycosylation sites (located at residues 72, 168, and 269 in FIG. la) . Only the first site is conserved in TGF-^1, and lies within a larger block of conserved residues, suggesting that this potential glycosylation site has important
structural and/or functional characteristics.
After removal of the signal sequence, the TGF-^2 precursor would contain either 31 or 59 amino acids more than its TGF-βΙ counterpart. An additional cysteine residue in TGF-^2 is located just upstream of a large region of non homologous amino acids that precedes the mature sequence. As with TGF-/S1, the cleavage site of the mature TGF-/92 protein occurs just after a region of 4-5 basic amino acids
as shown in FIG. 2A. The mature region contains nine cysteines. Conservation of 7 of the 9 cysteines is
characteristic for the different members of the TGF-β family. Hydropathy analyses of TGF-01 and TGF- 2 reveal similar patterns in both the precursor and mature regions with both proteins being generally hydrophilic in nature (data not shown) .
Figure 3A shows a Northern blot analysis using pPC-21 to probe polyadenylated RNA from BSC-40 (an African green monkey kidney cell line) and tamoxifen-treated PC-3 cells. PC-3 cells contain three major TGF-92 -specific mRNA species of 4.1, 5.1 and 6.5 kb in size (FIG. 3A, lane 2) ; BSC-40 cells contain predominantly the 4.1 and 6.5 kb transcripts and lesser amounts of the 5.1 kb RNA (FIG. 3A, lane 1) .
Note that the pPC-21 probe does not detect the 2.5 kb TGF-^l-specific mRNA species present in this cell line under the hybridization conditions used here. These results and previous observations (Sharpies et al . , 1987, DNA 6:239-244) suggest that BSC-40 cells contain both TGF-βΙ- and'TGF-£2-specific mRNA's. In order to demonstrate this more clearly, Northern blots were hybridized to a mixture containing equal amounts of TGF-01 and TGF- 2 probes radiolabelled to the same specific activity. Lane 1 of FIG. 3B shows that BSC-40 cells contain the 2.5kb TGF-^l-specific mRNA as well as the 4.1 and 6.5kb TGF-Beta 2 mRNA species: lane 2 of figure 3B shows that tamoxifen treated PC-3 cells also contain the 2.5kb TGF-^l-specific mRNA. FIG. 3B also demonstrates that tamoxifen-treated PC-3 cells contain more TGF-^l-specific than TGF-/92 -specific message.
The identification of TGF-/92-specific cDNA clones has enabled us to screen for TGF- 2 mRNA in various cell lines. The northern blot shown in FIG. 4 shows that TGF-92-specific transcripts could be detected in HBLIOO (a normal epithelial cell line derived from human milk, obtained from Dr. Greg Schultz) , MCF-7 (a human mammary carcinoma cell line), SK-
MEL 28 (a melanoma cell line), and KB cells; (a
nasopharyngeal carcinoma cell line) contain very low levels of TGF-02 mRNA.
7. EXAMPLE: cDNA CLONING OF TGF-BETA-2
PRECURSOR FROM BSC-40 CELLS , ,
The following examples describe the cDNA cloning of
TGF-/92 coding sequences from the African green monkey kidney cell line, BSC-40, shown to contain TGF-/92. specific mRNAs
(Section 6, supra) . The results indicate that simian TGF- β2 , like human TGF-,02, is synthesized as one of at least two longer precursors from which the mature TGF-^2 molecule is derived by proteolytic cleavage.
7.1. MATERIALS AND METHODS
The following procedures were used to clone cDNAs encoding simian TGF-/92 precursor.
7.1.1. GROWTH OF CELLS AND RNA EXTRACTION
BSC-40 cells were grown in Dulbecco's modified Eagle medium containing 10% fetal calf serum. Polyadenylated RNA was isolated by oligo[dT] -cellulose chromatography as described (Purchio and Fareed, 1979, J. Virol. 29:763-769) .
7.1.2. CDNA LIBRARY CONSTRUCTION AND SCREENING
Double-stranded cDNA was synthesized from BSC-40 polyadenylated RNA as described (Maniatis et al., 1982, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 371-372) and after treatment with EcoRI methylase was ligated to
oligonucleotide linkers containing an EcoRI restriction enzyme recognition site (EcoRI linkers) . The cDNA was digested with EcoRI and fractionated by chromatography on Sephacryl S-1000. cDNA fractions of greater than 750 base pairs were pooled and ligated into lambda gtlO which had
been cut with EcoRI (Davis et al., 1980, A Manual for
Genetic Engineering: Advanced Bacterial Genetics; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) , packaged (Grosveld et al., 1981, Gene 13:227-237) and plated on E. coli Cg00 rK~mK+hfl. The library was screened by plague hybridization (Bentonet et al., 1977 ,- Science 196:180-182) to [32P] -labeled pPC-21 and pPC-14 probes. Clone pBSC-40- 16, which hybridized the pPC-21 probe, and clone pBSC-40-1, which hybridized the pPC-14 probe, were isolated and
subcloned into pEMBL. The TGF-,52 coding sequence of pBSC- 40-1 was determined by sequencing both strands using the dideoxy chain-termination method (Sanger et al., 1977, Proc. Natl. Acad. Sci. USA 74 : 5463-546*7) . pBSC-40-16 was partially sequenced. ... ·.
7.2, RESULTS
Two clones were obtained from a BSC-40 cDNA library which hybridized alternatively to probes constructed from the human TGF-^2-442 and TGF-^2-414 precursor coding
sequences .
Clone pBSC-40-16, which hybridized to the TGF-/92-442 probe, was sequenced over a 150 nucleotide strech
(nucleotides 300 to 450 in FIG. la) expected to contain the coding sequence for the 29 amino acid segment from positions 346 to 432 in FIG. la. The results show that, over this region, pBSC-40-16 encodes an amino acid sequence which is identical to the corresponding sequence in the human TGF- 2-442 cDNA clone, pPC-21, and suggest that pBSC-40-16 encodes a 442 amino acid TGF-02 precursor.
Clone pBSC-40-1, which hybridized to the TGF-beta 2-414 probe, was sequenced over the entire coding region. The results show that this clone encodes a 414 amino acid TGF-92 precursor which is identical to the human TGF-/92-442
precursor except that. amino acid residues 116 through 144 human TGF- 2-442 are deleted and replaced by a single
Asparagine residue. At the nucleotide level, pBSC-40-1 differs from human TGF-/92-442 in the deletion region:
nucleotides 346 through 432 in FIG. la are deleted and replaced by the codon for Asparagine, AAT. Except for 13 silent base changes, the two structures are otherwise perfectly homologous over the rest of the coding sequence.
8. EXAMPLE: EXPRESSION OF TGF-02
The following examples describe the expression of mature, biologically active TGF-02 in Chinese Hamster Ovary cells (CHO cells) transfected with a recombinant plasmid containing the coding sequence for mature human TGF-02 ligated down-stream and in-frame with the coding sequence for the simian TGF-01 precursor,,, under the regulatory control of the SV40 promoter sequences. The construct directed the synthesis and secretion of mature, biologically active TGF-02 at a level of about 0.5 mg/L.
8.1. MATERIALS AND METHODS
8.1.1. CELL CULTURE
Dihydrofolate reductase (dhfr) -deficient Chinese
Hamster Ovary (CHO) cells (Urlaub and Chasin, 1980 Proc. Natl. Acad. Sci. U.S.A. 77:4216) were propagated in Ham's F-12 medium (Gibco Laboratories, NY) supplemented with 10% fetal bovine serum (FBS) and 150 ug/ml of L-proline.
Penicillin and streptomycin were included at 100 U/ml and 100 ug/ml, respectively. CHO transfectants were grown in Dulbecco's modified Eagle's medium containing the same supplements as those listed above. CHO cells and their derivatives were routinely passaged by trypsinization at a 1:5 splitting ratio.
Methotrexate (Sigma, MO) was prepared at a stock concentration of 10 mg/ml in water. Dilute NaOH (0.2 M) was added in order to solubilize the drug (final pH of 6) . The
stock was filter-sterilized and stored atr-:;'-20"C. Stock solutions of methotrexate in media (100 uM) were kept at 4°C for no longer than 1 month.
8.1.2. DNA MANIPULATIONS AND PLASMID CONSTRUCTIONS
Restriction enzymes, T4 DNA ligase - calf intestinal phosphatase, the Klenow fragment of DNA polymerase I and other DNA reagents were purchased from Bethesda Research Laboratories, MD. Standard DNA manipulations were performed as outlined in Maniatis, T., et al., 1982, Molecular
Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York.
Plasmid pSV2 (^l-TGF-dhfr) , which contains the simian TGF-βΙ cDNA and thev-mouse dhfr gene in tandem as well as intervening SV40 sequences, was constructed as described (Gentry et al., 1987, Mol. Cell. Biol. 7:3418) .
Plasmid pSV2/ l-/92/dhfr was constructed as outlined in .Section 8.2, infra.
8.1.3. DNA TRANSFECTIONS
Approximately 24 hours after seeding 106 dhfr-deficient CHO cells onto 100 mm dishes, the cultures were transfected with 20 ug of Ndel linearized pSV2- (/91-TGF-dhfr) plasmid as a calcium phosphate precipitate (Wigler, M. , et al., 1979, Proc. Natl. Acad. Sci. U.S.A. 76:1373-1376) .
Briefly, 20 ug of linearized DNA was added to 1 ml of 250 mM sterile CaCl2. A 1 ml portion of 2X HEPES solution (280 mM NaCl, 50 mM HEPES, 1.5 mM sodium phosphate, pH 7.1) was then added dropwise, and the mixture was allowed to sit on ice for 30 minutes. The precipitate was then dispersed dropwise over the cells containing 10 ml of the F12 media. After incubation at 37 'C for 4 hours, the media was removed and replaced with 10 ml of F12 media containing 25% glycerol for 90 seconds at room temperature. Cells were rinsed once with 20 ml of F12 media and incubated in the nonselective F12
media (20 ml) for an additional 48 hours. Selectio for dhfr-expressing transfectants was accomplished by replacing the media with DME supplemented with 10% dialyzed FBS (Gibco, N.Y.) and 150 ug/ml L-proline. Colonies were observed after culturing the cells 10-14 days in the selection media. Ten colonies were aspirated by a -pasteur pipet and expanded.
8.1.4. SELECTION OF METHOTREXATE RESISTANT CELLS
Dihydrofolate reductase (dhfr) -amplified cells were derived from the primary transfectants essentially , as described (Gasser, C.S. and Schimke, R.T. , 1986, J. Biol.
Chem. 261:6938-6946). After expansion, 10 cells were seeded onto 100 mm dishes and adapted to increasing '■ concentrations of methotrexate. The plate containing visible colonies at the highest methotrexate concentration was trypsinized and adapted to that concentration of methotrexate for at least two additional 1:5 cell passages.
Cells (10 ) were then seeded onto 100 mm dishes in 5 times the concentration of methotrexate. The dish containing visible colonies was again trypsinized and adapted in the methotrexate containing medium. Cells were frozen back at various stages of amplification in media containing 40% FBS, 10% dimethyl sulfoxide and 50% DMEM. Methotrexate was not included in the freezing media.
8.1.5. GROWTH INHIBITION ASSAY
Mink lung epithelial cells, Mv 1 Lu (Accession Number CCL-64, American Type Culture Collection), which are
extremely sensitive to TGF-^1, were utilized for the growth inhibition assay. The assay was performed using the
125 . 125
thymidine analog 5'-[ I] -iodo-2 'deoxyundine ( IdU) to assess DNA synthesis. One unit of activity was defined as
125 the amount required to inhibit 50% incorporation of IdU compared to untreated CCL-64 cells.
-
To assay transfected cells for secretion of active TGF-02, serum free supernatants were collected from one 24- hour collection on confluent cultures of cells and dialyzed extensively against 0.2 M acetic acid. The acetic acid was removed by lyophilization and the sample was re-dissolved in sterile complete culture medium for assays.
8.2. CONSTRUCTION OF TGF-BETA 1/TGF-BETA 2 HYBRID
PRECURSOR GENE FOR TGF-BETA 2 EXPRESSION
A hybrid TGF-beta precursor gene consisting of simian
TGF-Beta 1 precursor coding and 5' untranslated sequences joined in-frame with human TGF-Beta 2 mature coding and 3' untranslated sequences was constructed as illustrated in
FIG. lc.
pPC-21 was first digested with EcoRI filled-in with Klenow enzyme, the 2.3 Kb fragment ligated into Hindi
digested pEMBL, and used to transform E^ coli . Two clones, pPC-21/HincII+ and pPC-21/HincII~, having inserts in
opposite orientations, were used to generate overlapping
ExoIII digest fragments by digesting both with Sstl and
BamHI followed by ExoIII digestion, Klenow repair,
religation of the DNA, and transformation of E^ coli. Two clones, Exo 5.9 and Exo 25C were found to contain different lengths of 5' and 3' sequences, respectively, and were subcloned into pEMBL to generate pEMBL 5.9 and pEMBL 25C.
pEMBL 5.9 was digested with Hind!II , blunt ended with Klenow enzyme, digested with Kpnl , and the 0.6 Kb fragment (fragment 1) was isolated. Exo 25C was digested with EcoRI and Kpnl and the 1.1 Kb fragment (fragment 2) was isolated. pGS62 was digested with BamHI , filled in with Klenow enzyme, digested with EcoRI and ligated to fragments 1 and 2 (pGS62 was derived from pGS20 (Mackett et al., 1984, J. Virol.
49:857) by deletion of a single EcoRI site). The mixture was used to transform E. coli and pGS62/CIFB was isolated.
pGS62/CIFB was digested with Pstl and EcoRI and the 1600 bp fragment was isolated and further digested with
XhoII . The resulting 400 bp XhoII-EcoRI fragment was isolated (fragment 3). pSV2-beta-TGF (Gentry et al., 1987, Mol. Cell. Biol. 7:3418) was digested with Apal and EcoRI and the large 3000 bp fragment was isolated (fragment 4) .
Two complimentary strands of DNA with the sequences shown below were synthesized, phosphorylated, annealed and ligated to fragments '3' and '4' described above.
'5 CAA CAT CTG CAA AGC TCC CGG CAC CGC CGA GCT TTG
GAT GCG GCC TAT TGC TTT AGA AAT GTG CAG GAT AAT TGC TGC CTA CGT CCA CTT TAC ATT GAT TTC AAG AGG 3'
' GATC CCT CTT GAA ATC AAT GTA AAG TGG ACG TAG GCA
GCA ATT ATC CTG CAC ATT TCT AAA GCA ATA GGC CGC ATC CAA AGC TCG GCG GTG CCG GGA GCT TTG CAG ATG TTG GGCC 3'
The ligation mixture was used to transform E^ coli and ρβ1/β2 was isolated.
Plasmid ρβ!/β2 was digested with EcoRI, filled in with the Klenow fragment of DNA polymerase I, cut with Hindlll and the 1600 bp fragment was isolated: pSV2, 01/02 was constructed by inserting this fragment into pSV2, neo which had been previously digested with Hindlll and Hpal to eliminate the neo gene.
pSV2, 01/02 was digested with Pvul and EcoRI , filled in with Klenow enzyme, digested with Ndel and the 2.6 kb
(approx.) Ndel-EcoRI fragment was isolated and ligated to pSV2,dhfr which had been digested with Ndel and PvulI . The ligation mixture was used to transform E^ coli and pSV2/01-02/dhfr was isolated.
8.3. EXPRESSION OF TGF-BETA 2 IN CHO CELLS
pSV/,91-,32/dhfr was used to transfect dhfr-deficient CHO cells and dhfr-amplified cells were derived from the primary transfectants as described in Materials and Methods, supra .
Positive clones were identified by bioassay (inhibition of mink lung epithelial cells (CCL-64) as described (Gentry et al . , 1987, Mol. Cell, Biol. 7:3418). Recombinant
proteins were also detected by Western blotting using an anti-peptide antisera made against the sequence NH2- YNTINPEASASPC-COOH (Gentry et al., 1987, Mol. Cell, Biol. 7:3418) which is present in mature TGF-beta 2.
One line, 1β9 , 12.5, was found to secrete 240 ng/ml
TGF-,32 (FIG. 5) . This line was then cloned by limiting dilution in 96 well plates. One clone, 109, 12.5, cl 36, produced approximately 500 ng/ml (FIG. 5) .
Analysis of the protein secreted by this clone by
Western blotting using anti-peptide antiserum is shown in FIG. 6 . revealing the presence of the mature 24kd TGF-,52 dimer as well as the larger (approx. 90kd) precursor form.
9. DEPOSIT OF MICROORGANISMS
The following microorganisms have been deposited either with the Agricultural Research Culture Collection, Northern Regional Research Center (NRRL) or with the American Type Culture Collection (ATCC) and have been assigned the following accession numbers : ...
Microorganism Plasmid Accession No. Depository
Escherichia coli HB1.01 pPC-21 B-18256 NRRL
Escherichia coli HB101 pPC-14 B-18333 "
Escherichia coli HB101 pBSC-40-1 B-18335
Escherichia coli HB101 pBSC-40-16. B-18334 "
Chinese Hamster Ovary pSV/ 01 - 02 /dhfr CRL 9800 , ATCC
Claims (20)
1. A method of producing a protein having transforming growth factor-/?2 (TGF-/32) activity comprising: (a) culturing a eucaryotic cell containing a recombinant vector comprising a nucleotide sequence encoding a protein having transforming growth factor-/32 activity, said protein being a mature TGF- 32, a TGF-/32 precursor or a hybrid TGF- /31/TGF-/32 and comprising an amino acid sequence from about amino acid residue number 331 to 442 in the amino acid seqeunce depicted in Fig. la, the nucleotide sequence being under the control of a second nucleotide sequence that regulates gene expression so that a peptide or protein having TGF-/32 activity is produced by the eucaryotic cell; and (b) recovering the protein having TGF-/32 activity from the culture.
2. The method according to Claim 1 in which the nucleotide sequence encoding a protein having transforming growth factor- 32 activity comprises the nucleotide sequence substantially as depicted in Fig. la from nucleotide number 1 to 1339.
3. The method according to Claim 1 in which the nucleotide sequence encoding a protein having transforming growth factor-/32 activity comprises the nucleotide sequence substantially as depicted in Fig. la from nucleotide number 991 to 1326.
4. The method according to Claim 1 in which the nucleotide sequence encoding a protein having transforming growth factor-/32 activity comprises the nucleotide sequence substantially as depicted in Fig. lb from nucleotide number -70 to 1755. - 38 - 103749/3
5. The method according to Claim 1 in which the eucaryotic cell comprises a Chinese Hamster Ovary cell.
6. The method according to Claim 1 in which the second nucleotide sequence which controls gene expression comprises an SV40 promoter.
7. The method according to Claim 1 in which the second nucleotide sequence comprises a promoter and a sequence encoding a selectable marker for which the eucaryotic cell is deficient, so that the eucaryotic cell containing the nucleotide sequence encoding a protein having transforming growth factor-/?2 activity can be identified.
8. The method according to Claim 6 in which the selectable marker comprises dihydrofolate reductase.
9. The method according to Claim 7 further comprising exposing the eucaryotic cell to methotrexate, so that resistant colonies are selected which contain amplified levels of the coding sequences for dihydrofolate reductase and for a protein having transforming growth factor-/32 activity.
10. The method according to Claim 8 in which the eucaryotic cell comprises a dihydrofolate reductase-deficient Chinese Hamster Ovary cell.
11. A method according to Claim 1 for producing a protein having transforming growth factor-/32 activity, comprising (a) culturing transfectant CRL 9800 as deposited with the ATCC; and (b) recovering the protein having transforming growth factor-/32 activity from the culture.
12. The method according to Claim 10 in which the transfectant is cultured in the presence of methotrexate.
13. A eucaryotic cell for use in the method of Claim 1 containing a recombinant vector comprising a nucleotide sequence encoding a protein having transforming growth factor-/32 activity under the control of a second - 39 - 103749/3 nucleotide sequence that regulates gene expression so that the eucaryotic cell produces a protein having transforming growth factor-/32 activity.
14. The eucaryotic cell according to Claim 12 in which the nucleotide sequence encoding the protein having transforming growth factor-/32 activity comprises the nucleotide sequence substantially as depicted in Fig. lb from nucleotide number -70 to 1755.
15. The eucaryotic cell according to Claim 12 which comprises a Chinese Hamster Ovary cell.
16. The eucaryotic cell according to Claim 12 in which the second nucleotide sequence which controls gene expression comprises an SV40 promoter.
17. The eucaryotic cell according to Claim 12 in which the second nucleotide sequence comprises a promoter and a sequence encoding a selectable marker for which the eucaryotic cell is deficient, so that eucaryotic cell containing, the nucleotide sequence encoding a protein having transforming growth factor-/32 activity can be identified.
18. The eucaryotic cell according to Claim 16 in which the selectable marker comprises dihydrofolate reductase.
19. The eucaryotic cell according to Claim 17 which comprises a dihydrofolate reductase-deficient Chinese Hamster Ovary cell.
20. An eucaryotic cell line according to Claim 12 designated CRL 9800 as deposited with the ATCC. For the Applicants, DR. REINHOLD COHN AND PARTNERS 87S98aaims TG/be/4.8.1994
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10675287A | 1987-10-06 | 1987-10-06 | |
US14826788A | 1988-01-25 | 1988-01-25 | |
US23406588A | 1988-08-18 | 1988-08-18 | |
IL87877A IL87877A0 (en) | 1987-10-06 | 1988-09-29 | Cloning and expression of transforming growth factor beta2 |
Publications (1)
Publication Number | Publication Date |
---|---|
IL103749A true IL103749A (en) | 1994-12-29 |
Family
ID=27452342
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL10374988A IL103749A (en) | 1987-10-06 | 1988-09-29 | Method for production of transforming growth factor beta 2 |
Country Status (1)
Country | Link |
---|---|
IL (1) | IL103749A (en) |
-
1988
- 1988-09-29 IL IL10374988A patent/IL103749A/en not_active IP Right Cessation
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5221620A (en) | Cloning and expression of transforming growth factor β2 | |
Gentry et al. | Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells | |
KR100214740B1 (en) | Dna sequences encoding bmp-6 proteins | |
AU654316B2 (en) | Osteogenic factor | |
AU669331B2 (en) | TGF-beta 1/beta 2: a novel chimeric transforming growth factor-beta | |
AU634733B2 (en) | Tgf-beta 1/beta 2: a novel chimeric transforming growth factor-beta | |
AU7722494A (en) | Activin receptors-like kinase (alk), belonging to the tgf receptor family and/or to the bmp receptor family | |
Hallböök et al. | Production and characterization of biologically active recombinant beta nerve growth factor | |
IE60918B1 (en) | Cloning and expression of transforming growth factor beta 2 | |
AU630075B2 (en) | Cloning and expression of transforming growth factor 2b | |
IL103749A (en) | Method for production of transforming growth factor beta 2 | |
JPH09295945A (en) | Production of mature type bone inducing factor | |
JPH0144317B2 (en) | ||
JPH0556783A (en) | Cloning and expression of transforming growth factor beta 2 | |
LU87361A1 (en) | New DNA sequence encoding transforming growth factor beta 2 - used for large scale expression in eucaryotic cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RH1 | Patent not in force |