IE911267A1 - 2(5h)-furanones substituted in the 5 and or in the 4¹position, as anti-inflammatory agents - Google Patents
2(5h)-furanones substituted in the 5 and or in the 4¹position, as anti-inflammatory agentsInfo
- Publication number
- IE911267A1 IE911267A1 IE126791A IE126791A IE911267A1 IE 911267 A1 IE911267 A1 IE 911267A1 IE 126791 A IE126791 A IE 126791A IE 126791 A IE126791 A IE 126791A IE 911267 A1 IE911267 A1 IE 911267A1
- Authority
- IE
- Ireland
- Prior art keywords
- compounds
- alkyl
- formula
- hydrogen
- carbons
- Prior art date
Links
- 239000002260 anti-inflammatory agent Substances 0.000 title description 4
- 229940121363 anti-inflammatory agent Drugs 0.000 title description 4
- 150000001875 compounds Chemical class 0.000 claims abstract description 129
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 56
- 239000001257 hydrogen Substances 0.000 claims abstract description 31
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 31
- 125000003710 aryl alkyl group Chemical group 0.000 claims abstract description 18
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 150000001345 alkine derivatives Chemical class 0.000 claims abstract description 9
- 125000002947 alkylene group Chemical group 0.000 claims abstract description 8
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims abstract description 7
- -1 Compounds Compounds Chemical class 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 13
- 150000002431 hydrogen Chemical class 0.000 claims description 11
- 239000008194 pharmaceutical composition Substances 0.000 claims description 11
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 8
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 5
- 125000001246 bromo group Chemical group Br* 0.000 claims description 4
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 4
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 claims description 2
- 229910052794 bromium Inorganic materials 0.000 claims description 2
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 95
- 239000000203 mixture Substances 0.000 description 37
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 36
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 36
- 101150041968 CDC13 gene Proteins 0.000 description 35
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 35
- 239000000243 solution Substances 0.000 description 35
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 33
- 238000001704 evaporation Methods 0.000 description 28
- 230000008020 evaporation Effects 0.000 description 28
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 24
- 239000002253 acid Substances 0.000 description 24
- 239000003921 oil Substances 0.000 description 24
- 238000006243 chemical reaction Methods 0.000 description 22
- 238000005481 NMR spectroscopy Methods 0.000 description 21
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 21
- 239000003208 petroleum Substances 0.000 description 20
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 18
- 235000019341 magnesium sulphate Nutrition 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 17
- RHDGNLCLDBVESU-UHFFFAOYSA-N but-3-en-4-olide Chemical compound O=C1CC=CO1 RHDGNLCLDBVESU-UHFFFAOYSA-N 0.000 description 16
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 14
- 239000000377 silicon dioxide Substances 0.000 description 14
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical group C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 229910001868 water Inorganic materials 0.000 description 13
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 12
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 12
- 150000003839 salts Chemical class 0.000 description 12
- 239000000284 extract Substances 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 9
- 238000001816 cooling Methods 0.000 description 9
- 230000005764 inhibitory process Effects 0.000 description 9
- 239000002904 solvent Substances 0.000 description 9
- 108010058864 Phospholipases A2 Proteins 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- FGJIDQWRRLDGDB-CPIXEKRISA-N manoalide Chemical compound C=1([C@@H](O[C@H](CC=1)C=1[C@@H](OC(=O)C=1)O)O)CC\C=C(/C)CCC1=C(C)CCCC1(C)C FGJIDQWRRLDGDB-CPIXEKRISA-N 0.000 description 8
- FGJIDQWRRLDGDB-GMKZXUHWSA-N manoalide Natural products CC(=CCCC1=CC[C@@H](O[C@H]1O)C2=CC(=O)O[C@H]2O)CCC3=C(C)CCCC3(C)C FGJIDQWRRLDGDB-GMKZXUHWSA-N 0.000 description 8
- 239000012279 sodium borohydride Substances 0.000 description 8
- 229910000033 sodium borohydride Inorganic materials 0.000 description 8
- 102100037611 Lysophospholipase Human genes 0.000 description 7
- 125000004432 carbon atom Chemical group C* 0.000 description 7
- 239000011981 lindlar catalyst Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 239000000543 intermediate Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 125000001424 substituent group Chemical group 0.000 description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 5
- 229910052786 argon Inorganic materials 0.000 description 5
- 229910052791 calcium Inorganic materials 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 229940125898 compound 5 Drugs 0.000 description 5
- 150000002148 esters Chemical class 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 150000002576 ketones Chemical class 0.000 description 5
- 239000012074 organic phase Substances 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 238000005160 1H NMR spectroscopy Methods 0.000 description 4
- DUAZKLYNTLDKQK-UHFFFAOYSA-N 5-hydroxy-2(5h)-furanone Chemical group OC1OC(=O)C=C1 DUAZKLYNTLDKQK-UHFFFAOYSA-N 0.000 description 4
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 4
- 239000003810 Jones reagent Substances 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- MZRVEZGGRBJDDB-UHFFFAOYSA-N N-Butyllithium Chemical compound [Li]CCCC MZRVEZGGRBJDDB-UHFFFAOYSA-N 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- 150000001299 aldehydes Chemical class 0.000 description 4
- 239000003659 bee venom Substances 0.000 description 4
- 239000012230 colorless oil Substances 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 4
- 230000008025 crystallization Effects 0.000 description 4
- KSMVZQYAVGTKIV-UHFFFAOYSA-N decanal Chemical compound CCCCCCCCCC=O KSMVZQYAVGTKIV-UHFFFAOYSA-N 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 4
- 230000004054 inflammatory process Effects 0.000 description 4
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 4
- 238000001953 recrystallisation Methods 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 4
- ONBQEOIKXPHGMB-VBSBHUPXSA-N 1-[2-[(2s,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-4,6-dihydroxyphenyl]-3-(4-hydroxyphenyl)propan-1-one Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=CC(O)=C1C(=O)CCC1=CC=C(O)C=C1 ONBQEOIKXPHGMB-VBSBHUPXSA-N 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical class [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 3
- 108090000312 Calcium Channels Proteins 0.000 description 3
- 102000003922 Calcium Channels Human genes 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 201000004681 Psoriasis Diseases 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 102000014384 Type C Phospholipases Human genes 0.000 description 3
- 108010079194 Type C Phospholipases Proteins 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 150000001412 amines Chemical class 0.000 description 3
- 230000001028 anti-proliverative effect Effects 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- 229940125904 compound 1 Drugs 0.000 description 3
- 229940126142 compound 16 Drugs 0.000 description 3
- 239000003937 drug carrier Substances 0.000 description 3
- 210000005069 ears Anatomy 0.000 description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 3
- FMVJYQGSRWVMQV-UHFFFAOYSA-N ethyl propiolate Chemical compound CCOC(=O)C#C FMVJYQGSRWVMQV-UHFFFAOYSA-N 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- YFHXZQPUBCBNIP-UHFFFAOYSA-N fura-2 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=3OC(=CC=3C=2)C=2OC(=CN=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 YFHXZQPUBCBNIP-UHFFFAOYSA-N 0.000 description 3
- 150000002241 furanones Chemical group 0.000 description 3
- 125000003844 furanonyl group Chemical group 0.000 description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 3
- 239000003018 immunosuppressive agent Substances 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 150000002596 lactones Chemical class 0.000 description 3
- 150000007522 mineralic acids Chemical class 0.000 description 3
- 239000002674 ointment Substances 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- MOOYVEVEDVVKGD-UHFFFAOYSA-N oxaldehydic acid;hydrate Chemical compound O.OC(=O)C=O MOOYVEVEDVVKGD-UHFFFAOYSA-N 0.000 description 3
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 3
- 150000003906 phosphoinositides Chemical class 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 235000011149 sulphuric acid Nutrition 0.000 description 3
- AOSZTAHDEDLTLQ-AZKQZHLXSA-N (1S,2S,4R,8S,9S,11S,12R,13S,19S)-6-[(3-chlorophenyl)methyl]-12,19-difluoro-11-hydroxy-8-(2-hydroxyacetyl)-9,13-dimethyl-6-azapentacyclo[10.8.0.02,9.04,8.013,18]icosa-14,17-dien-16-one Chemical compound C([C@@H]1C[C@H]2[C@H]3[C@]([C@]4(C=CC(=O)C=C4[C@@H](F)C3)C)(F)[C@@H](O)C[C@@]2([C@@]1(C1)C(=O)CO)C)N1CC1=CC=CC(Cl)=C1 AOSZTAHDEDLTLQ-AZKQZHLXSA-N 0.000 description 2
- SZUVGFMDDVSKSI-WIFOCOSTSA-N (1s,2s,3s,5r)-1-(carboxymethyl)-3,5-bis[(4-phenoxyphenyl)methyl-propylcarbamoyl]cyclopentane-1,2-dicarboxylic acid Chemical compound O=C([C@@H]1[C@@H]([C@](CC(O)=O)([C@H](C(=O)N(CCC)CC=2C=CC(OC=3C=CC=CC=3)=CC=2)C1)C(O)=O)C(O)=O)N(CCC)CC(C=C1)=CC=C1OC1=CC=CC=C1 SZUVGFMDDVSKSI-WIFOCOSTSA-N 0.000 description 2
- QFLWZFQWSBQYPS-AWRAUJHKSA-N (3S)-3-[[(2S)-2-[[(2S)-2-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-methylbutanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-[1-bis(4-chlorophenoxy)phosphorylbutylamino]-4-oxobutanoic acid Chemical compound CCCC(NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)CCCCC1SC[C@@H]2NC(=O)N[C@H]12)C(C)C)P(=O)(Oc1ccc(Cl)cc1)Oc1ccc(Cl)cc1 QFLWZFQWSBQYPS-AWRAUJHKSA-N 0.000 description 2
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- MFGALGYVFGDXIX-UHFFFAOYSA-N 2,3-Dimethylmaleic anhydride Chemical compound CC1=C(C)C(=O)OC1=O MFGALGYVFGDXIX-UHFFFAOYSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
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- VPSRLGDRGCKUTK-UHFFFAOYSA-N fura-2-acetoxymethyl ester Chemical compound CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C)C=C1OCCOC(C(=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)=CC2=C1OC(C=1OC(=CN=1)C(=O)OCOC(C)=O)=C2 VPSRLGDRGCKUTK-UHFFFAOYSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 150000004795 grignard reagents Chemical class 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229940125721 immunosuppressive agent Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- INQOMBQAUSQDDS-UHFFFAOYSA-N iodomethane Chemical compound IC INQOMBQAUSQDDS-UHFFFAOYSA-N 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000012948 isocyanate Substances 0.000 description 1
- 150000002513 isocyanates Chemical class 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229910003002 lithium salt Inorganic materials 0.000 description 1
- 159000000002 lithium salts Chemical class 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 235000001055 magnesium Nutrition 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 235000014380 magnesium carbonate Nutrition 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- NXPHGHWWQRMDIA-UHFFFAOYSA-M magnesium;carbanide;bromide Chemical compound [CH3-].[Mg+2].[Br-] NXPHGHWWQRMDIA-UHFFFAOYSA-M 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- FGJIDQWRRLDGDB-GFOLISOJSA-N manoalide Chemical compound C=1([C@H](O[C@H](CC=1)C=1C(OC(=O)C=1)O)O)CC\C=C(/C)CCC1=C(C)CCCC1(C)C FGJIDQWRRLDGDB-GFOLISOJSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- GRVDJDISBSALJP-UHFFFAOYSA-N methyloxidanyl Chemical compound [O]C GRVDJDISBSALJP-UHFFFAOYSA-N 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 210000002464 muscle smooth vascular Anatomy 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 230000009826 neoplastic cell growth Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical class [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012746 preparative thin layer chromatography Methods 0.000 description 1
- 150000003138 primary alcohols Chemical class 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000027425 release of sequestered calcium ion into cytosol Effects 0.000 description 1
- 230000000979 retarding effect Effects 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 125000000547 substituted alkyl group Chemical group 0.000 description 1
- 125000005346 substituted cycloalkyl group Chemical group 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 229940037128 systemic glucocorticoids Drugs 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000005270 trialkylamine group Chemical group 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 229960000281 trometamol Drugs 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000003871 white petrolatum Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/26—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
- C07D307/28—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/26—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
- C07D307/30—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/58—One oxygen atom, e.g. butenolide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/60—Two oxygen atoms, e.g. succinic anhydride
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
- C07F9/65515—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a five-membered ring
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Pain & Pain Management (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Compounds of formula (1), and compounds of formula (2) in which R1 is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds or arylalkyne having one or more triple bonds; R2 is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds or arylalkyne having one or more triple bonds; R3 is H, alkyl of 1 to 20 carbons, arylalkyl, or halogene, and X is H or alkyl of 1 to 20 carbons, CO-X<*>, CO-O-X<*>,, CO-NH-X<*>,, or PO(OX<*>,)2 or PO(OX<*>,)X<*>,, where X<*>, independently is H, alkyl of 1 to 20 carbons, phenyl, or substituted phenyl, are disclosed. However, in the compounds of the invention illustrated by formula (2), R1 and R3 both cannot be hydrogen. The compounds have anti-inflammatory activity.
Description
2(5H)-FURANONES SUBSTITUTED IN THE 5 AND OR IN THE 4 POSITION, AS ANTI-INFLAMMATORY AGENTS
BACKGROUND OF THE INVENTION
1* Field of the Invention
The present invention is directed to novel 2(5H)furanones substituted in the 5 position and or in the 4 position, which compounds are active as anti-inflammatory agents. The present invention is also directed to pharmaceutical compositions which comprise one or more of the novel compounds of the invention, to the methods of using these pharmaceutical compositions, and to the chemical processes of making the novel compounds.
2. Brief Description of the Prior Art
Manoalide is a compound isolated from a marine sponge [E. D. de Silva et al., Tetrahedron Letters 21:1611-1614 (1980)] which has anti-inflammatory, immunosuppressive and analgesic properties. Manoalide (Compound 1) the structure of which is shown below, includes a 5-hydroxy2(5H)-furanone moiety, attached in the 4-position of the furanone ring to the rest of the molecule. Certain analogs of manolide, such as aeco-manoalide (Compound 2) and dahydro-saco-manoalida (Compound 3) also have antiinflammatory activity. For further description of the biological activity of manoalide and some of its derivatives reference is made to United States Patent Nos. 4,447,445, 4,786,651, 4,789,749 and to European Patent Application No. 0 133 376 (published on February 20,
1985).
40020PCT
16791
manoalide
Compound 1
Synthetic analogs of manoalide, particularly analogs having various substituents on the furanone moiety of manoalide, are described in several applications for United States Letters Patent by the same inventor as in the present application, the following of which have been allowed and are expected to issue as United States Letters Patent:
Serial No. 259,225 filed on October 18, 1988;
Serial No. 281,126 filed on December 7, 1988. Published European Patent Application No. 0 295 056 discloses 4-substituted 5-hydroxy-2(5H)-furanones having
40020PCT
16791 anti-inflammatory, immunosuppressive and antiproliferative activity where the substituents in the 4 position are a variety 1-hydroxyalkyl, 1-acyloxy-alkyl and l-carbamoyloxy-alkyl groups.
United States Patent No. 4,855,320 discloses 5arylalkyl-4-alkoxy-2(5H)-furanones as anti-convulsive and anti-epileptic agents.
Published European Patent Application No. 0 209 274 discloses 4-alkyl-5-hydroxy-2(5H)-furanones as anti10 inflammatory and anti-allergy agents.
Chemical Abstracts Volume 107 236559t (1987) discloses 4-acyloxy 5-hydroxy-2(5H)-furanones.
SUMMARY OF THE INVENTION
The present invention covers compounds of Formula 1, and compounds of Formula 2
Formula 1 Formula 2
40020PCT
16791 in which Rx is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds or arylalkyne having one or more triple 5 bonds;
R2 is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds or arylalkyne having one or more triple bonds;
R3 is H, alkyl of 1 to 20 carbons, arylalkyl, or halogene, and
X is H or alkyl of 1 to 20 carbons, CO-X*, CO-O-X*,, CO-NH-X*, PO(OX*,)2 or PO(OX*,)X*,, where X*,independently is H, alkyl of 1 to 20 carbons, phenyl, or substituted phenyl. However, in the compounds of the invention illustrated by Formula 2, both Rx and Rj cannot be hydrogen.
The present invention also covers salts of the abovedefined compounds, formed with pharmaceutically acceptable acids or bases, as applicable.
In a second aspect the present invention relates to pharmaceutical formulations comprising one or more compounds of Formula 1 or of Formula 2, or both, (or pharmaceutically acceptable salts thereof) in admixture with a pharmaceutically acceptable excipient, for the purpose of treating certain conditions, syndromes or diseases in mammals, including humans. The compounds of the invention have anti-inflammatory, immunosuppressant and anti-proliferative activity. Therefore, the compounds are useful for treating in mammals (including humans) inflammation, rheumatoid arthritis, osteoarthritis, rheumatic carditis, ocular and dermal inflammatory diseases, autoimmune diseases such as allergic diseases,
40020PCT
16791 bronchial asthma and myasthenia gravis, and for suppressing unwanted immune responses and retarding proliferation of cell.
R,CHO ♦ HCSCCOjEt
BuLi
RjCHCOHJCSCCOjEt
Formula 3
Formula 4
1. H2 / Llndlar catalyst
2. acid
Formula 1 R2 = R3 = H
Reaction Schema 1
In still another aspect, the present invention relates to the processes of making the compounds of
Formula 1 and of Formula 2. In general terms, these processes, shown in Reaction Scheme 1, 2, 3 and 4 comprise the steps of reacting an aldehyde of Formula 3 with a
40020PCT
16791 lithium or magnesium salt derived from a propiolate ester, such as ethyl propiolate (Compound 4), to yield an Rxsubstituted 4-hydroxy —ynoate of Formula 4 (Rx defined as in connection with Formula 1 and Formula 2).
Hydroganation over a poisoned catalyst (Lindlar catalyst) converts the acetylenic (triple) bond into an olephinic (double) bond. This is followed by acid catalyzed cyclization to yield compounds of Formula 1 where R2 and Rj are hydrogen.
n,CH
Formula 4
R,CHC = CCC^
Formula 5
Jones reagent
R,COC=CCOjH v
Formula 6
H] / Lindlar catalyst
R,COCH=CHCO,H j
Reaction Scheme 2 cyclization
Formula 2 Rj Rj Β H
40020PCT 7 16791
In order to obtain compounds of Formula 2, as is shown in Reaction Scheme 2, the intermediate Rx~ substituted 4-hydroxy—ynoate of Formula 4 is saponified to obtain the corresponding free carboxylic acid of 5 Formula 5, which is thereafter oxidized (typically with Jones reagent) to yield the corresponding Rj^-substituted
4-oxo-alkynoic acid of Formula 6. The intermediate of Formula 6 is hydrogenated over a poisoned catalyst, typically Lindlar catalyst, to yield an alkenoic acid 10 intermediate which cyclizes, usually spontanously, to provide compounds of Formula 2 where R2, R3 and X are hydrogen. As is shown in Reaction Scheme 2, the compounds of Formula 2 can be reduced with a mild reducing agent, such as sodium borohydride to provide the corresponding compounds of Formula 1. A theoretical explanation for this reaction (although the present inventors do not wish to be bound by theory) is that the carbon in the 5position of the 5-hydroxy-2(5H)-furanone molecule is an aldehydic carbon, ring closed with the carboxylic acid group in the 2-position of the ring, and that the aldehydic carbon is reduced with sodium borhydride to a primary alcohol, which, thereafter, ring closes with the carboxylic acid to form a lactone of Formula 1.
Compounds of Formula 2 where the X substituent is 25 other than hydrogen, can be obtained by acylation, carbamate formation (through reaction with isocyanate) phosphorylation and the like, in accordance with procedures which are within the skill of the practicing organic chemist.
40020PCT 8 16791 R^COCHjR, 4 choco2h acid heat | r1cocr2=chco2h j Formula 7 5. Formula 8 /acid / heat XOH NaBH4 ρ==^ 2 acid0A0A„ Formula 2 X = alkyl R3 = H Formula 2 r3 = x = h Formula 1 R3 = H Br2Br— o^^/^ox H2O / acid Br._______ - /γ Formula 2 X = alkyl Formula 2
Reaction Scheme 3
40020PCT
16791
In order to obtain compounds of Formula 2 where neither Rx nor R2 are hydrogen, a ketone of Formula 7 (which bears the desired R^ and R2 groups) is condensed under acidic condition with glyoxylic acid (Compound 5), 5 as is shown in Reaction Scheme 3. An intermediate condensation product of Formula 8 usually is not isolated because it cyclizes during the condition of the condensation reaction to form the furanones of Formula 2 where the R^ and R2 groups are derived from the starting 10 ketone of Formula 7 and where X is hydrogen. Reduction of the 5-hydroxy-2(5H)-furanone compound of Formula 2 (X is H) with sodium borohydride, as shown in Reaction Scheme 3 results in removal of the 5-hydroxy group and yields the corresponding compound of Formula 1.
X5 Referring still to Reaction Scheme 3, compounds of
Formula 2 where Rj is hydrogen, are obtained when the starting material of Formula 7 is an aldehyde rather than a ketone, (in Formula 7 R^-H). In this case, the condensation reaction with glyoxylic acid (Compound 5) is 20 usually performed in the presence of morpholine hydrochloride. An alkyl substitutent for the 5-hydroxy function (in Formula 2 X=alkyl) is introduced into the molecule by reacting the 5-hydroxy-2(5H)-furanone derivative of Formula 2 with the appropriate alcohol (XOH) in the presence of acid. The resulting 5-alkoxy-2(5H)furanone derivative of Formula 2 where Rj is hydrogen, is brominated to yield the corresponding 3-bromo-5-alkoxy2(5H)-furanone derivative of Formula 2. The alkoxy substituent can be replaced with OH by reaction with aqueous acid.
40020PCT
Formula 9
Formula 10
R-,MgBr
BuLI
16791
Formula 11 h2
Formula 2
Formula 2
NaBH4
NaBH^
Formula 1
Formula 1
Reaction Scheme 4
40020PCT
16791
Reaction Scheme 4 summarizes a reaction sequence, in which compounds of the invention can be prepared where neither R2 nor R3 are hydrogen. In accordance with this scheme, a di-substituted maleic acid anhydride (Formula 9) 5 is reacted with the lithium salt of an alkyne (Formula 10) to provide a 5-alkynyl substituted 5-hydroxy-2(5H)furanone derivative (Formula 11). In Formula 10 -CC-RX symbolizes such a precursor of the group Rx which is readily converted by hydrogenation and or other reactions 10 within the skill of the practicing organic chemist, into the group Rx defined in connection with Formula 1 and Formula 2. Reaction Scheme 4 shows a hydrogenation step in which the olephinic bond of the intermediate of Formula 11 is partially or fully saturated to provide compounds of
Formula 2. Reduction of the compounds of Formula 2 with sodium borohydride provides compounds of Formula 1.
Another process of preparing compounds of the invention where neither R2 nor R3 are hydrogen, involves the reaction a di-substituted maleic acid anhydride of
Formula 9 with a Grignard reagent of the formula R^-MgBr to provide compounds of Formula 2 where X is hydrogen. Reduction of these compounds with sodium borohydride, yields compounds of Formula 1.
In another general aspect, the processes leading to the compounds of the invention may involve performance of routine chemical reactions (such as esterification, saponification of esters, oxidation of alcohols to ketones or aldehydes, formation of acetals, ketals, and lactones from aldehydes or ketones, and the like) which are well known to the practicing synthetic organic chemist.
When it is desired to substitute, for example acylate, the 5-hydroxy function of the compounds of Formula 2, an X group (as defined in connection with
40020PCT
16791 these formulas) can be introduced into the
-hydroxy-2(5H)-furanone compounds by conventional means.
As it was illustrated in connection with Reaction Scheme
3, introduction of an Z alkyl group into the compounds of
Formula 2 in reality is formation of an acetal, which can be accomplished by treating the respecting
-hydroxy-2(5H)-furanone with an alcohol ZOH in the presence of acid.
general Embodiments
Definitions
The terms ester, amine, amide, ether acetal, lactone and all other terms and terminology used here, (unless specifically defined in the present description) refer to and cover any compounds falling within the respective term as that term is classically used in organic chemistry.
Unless specifically noted otherwise, preferred esters are derived from the saturated aliphatic alcohols or acids of ten or fewer carbon atoms or from the cyclic or saturated aliphatic cyclic alcohols and acids of 5 to 10 carbon atoms. Particularly preferred aliphatic esters are those derived from lower alkyl acids or alcohols. Also preferred are the phenyl or lower alkylphenyl esters.
The term alkyl as used in the present description and claims includes straight chain alkyl groups, branched chain alkyl groups, cycloalkyl groups, alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups. Unless the number of carbons is otherwise specified, lower alkyl means the former broad definition of alkyl groups but with the restriction that the group has 1 to 6 carbon atoms.
The term long chain alkyl also means the former broad definition of alkyl groups but with the
16791
40020PCT restriction that the group has no less than 4 carbon atoms, and no more than approximately 25 carbon atoms.
Unless specifically noted otherwise, preferred amides are the mono- and di-substituted amides derived from the 5 saturated aliphatic radicals of ten or fewer carbon atoms, or the cyclic or saturated aliphatic-cyclic radicals of 5 to 10 carbon atoms.
Some of the compounds of the invention (Formula 1) contain a non-equilibrating chiral center in the 5 position of the furan ring. Other compounds of the invention may contain one or more additional chiral centers. Accordingly, the compounds of the invention may be prepared as mixtures of enantiomeric compounds (where the enantiomers may or may not be present in equal amounts) or as optically pure enantiomers. When there is more than one chiral center, the compounds of the invention may also be prepared as mixtures of diastereomers, or as pure diastereomers, and each diastereomer itself may be a mixture of enantiomers in 20 1:1, or other, ratios. Alternatively, each diastereomeric compound may be sterically and optically pure. However, all of the above-noted forms, including optically pure enantiomers and mixtures thereof, as well as all diastereomers, are within scope of the present invention.
Some of the compounds of the invention, for example those which contain olephinic double bonds in the side chains, may have cis and trans stereoisomers. The scope of the invention includes both pure stereoisomers as well as mixtures thereof.
A pharmaceutically acceptable salt may be prepared for any compound of this invention having a functionality capable of forming such salt, for examle an acid or an amine functionality. A pharmaceutically acceptable salt
16791
40020PCT may be any salt which retains the activity of the parent compound and does not impart any deleterious or untoward effect on the subject to which it is administered and in the context in which it is administered.
Such a salt may be derived from any organic or inorganic acid or base. The salt may be a mono or polyvalent ion. Of particular interest where the acid function is concerned are the inorganic ions, sodium, potassium, calcium, and magnesium. Organic amine salts may be made with amines, particularly ammonium salts such as mono-, di- and trialkylamines or ethanol amines. Salts ' may also be formed with caffeine, tromethamine and similar molecules. Where there is a nitrogen sufficiently basic as to be capable of forming acid addition salts, such may
I5 be formed with any inorganic or organic acids or alkylating agent such as methyl iodide. Preferred salts are those formed with inorganic acids such as hydrochloric acid, sulfuric acid or phosphoric acid. Any of a number of simple organic acids such as mono-, di- or tri-acid may also be used.
The preferred compounds of the present invention are, with reference to Formula 2 and with respect to the OX substituent in the 5-position of the furanone moiety, those where the substituent is hydroxy, methoxy or acetyloxy (X is H, or CH3O or CH3CO).
With respect to the Rx susbtituent in the 5 position of the 2(5H)-furanone molecule, the preferred compounds in accordance with the present invention are those where Rx is hydrogen, long chain alkyl, or arylalkyl. Compounds are particularly preferred in this regard where the Rx group is long chain alkyl which is straight chained, or where the Rx is arylalkyl containing a straight alkyl chain of 3 carbons.
16791
40020PCT
With respect to the R2 substituent in the 4-position of the 2(5H)-furanone molecule, the compounds of the invention are preferred where R2 is hydrogen, or alkyl group, particularly straight chain alkyl.
With respect to position 3 of the 2(5H)-furanones of the invention, compounds are preferred where R3 is H, methyl or bromo.
The most preferred compounds of the invention are those listed just below with reference to Formula l or 10 Formula 2: Formula 1, Compound 6: RX«CH3(CH2)g, R2=H, and R3=H, Formula 2, Compound 7: X=H? Rj^CH-j (CH2)8, R2»H, R3=H and Formula X, Compound 8: Rx—(CH2)3-C6h5,R2»ch3, and 15 r3=ch3j Formula 2, Compound 9: X=H, and RX«H, R2«CH3(CH2)7, R3=Br and Formula X, Compound XO: Rx—Η, R2—CH3(CH2)7 and R3sH.
The compounds of the present invention are useful in pharmaceutical compositions to produce anti-inflammatory, immunosuppressant and anti-proliferative activity. The diseases, syndromes or conditions of mammals (including humans) which can be treated with pharmaceutical compositions containing one or more compounds of the invention (or salts thereof) include: inflammation, rheumatoid arthritis, osteoarthritis, rheumatic carditis, ocular and dermal inflammatory diseases, autoimmune diseases such as allergic diseases, bronchial asthma and myasthenia gravis, unwanted immune responses and unwanted proliferation of cells, psoriasis, acne, atopic diseases and allergic conjunctivitis.
The activity of the compounds of this invention is demonstrated by inhibition of the enzyme phospholipase A2
16791
40020PCT in vitro and by reduction of inflammation in the mouse ear anti-inflammatory assay in vivo.
Activity of compounds of this invention may also be demonstrated by inhibition of phosphoinositide-specific phospholipase C. This activity has been reported for manoalide and may indicate anti-inflammatory utility. Bennett et al, Molecular Pharmacology 32:587-593 (1987).
Activity of the compounds may also be demonstrated by inhibition of ornithine decarboxylase, a rate limiting enzyme in cellular growth, which indicates use in treating psoriasis and neoplasis.
The compounds also modify calcium homeostasis. This activity is shown by effect on intracellular calcium levels in experiments using gastric glands, spleen cells, epithelial cells, GH3 cells, etc. Calcium is inhibited from entering through the plasma membrane calcium channels and calcium release from intracellular stores is also blocked. Modification of calcium homeostasis is expected to have application in diseases of the nervous system involving modification of membrane lipids or transmitter release (Parkinson's, Alzheimer's), diseases of the cardiovascular system involving application of cardiac or vascular smooth muscle contractility and platelet aggregation (hypertension, cardiac infarction and atherosclerosis), diseases of the gastrointestinal tract such as ulcer disease, diarrhea, motility due to secretion of acid or Cl, diseases of the kidney involving renal handling of fluid and electrolytes (metabolic acidosis, alkalosis), and disease of abnormal growth (neoplasia, psoriasis).
The compounds of this invention have activity which is similar to that of manoalide, that is the compounds appear to be devoid of the endocrine properties of the
40020PCT
16791 glucocorticoids while having anti-inflammatory and immunosuppressive properties.
In the methods of this invention, the compounds of the invention are administered to mammals, including humans, in an effective amount to produce the desired activity, preferably in an amount of about 0.05 to 100 mg per day per kilogram of body weight. The amount of the compound depends upon the disease or condition being treated, the severity thereof, the route of administration
1G and the nature of the host. The compounds may be administered topically, orally, parenterally or by other standard routes of administration.
Pharmaceutical compositions of this invention comprise compounds of Formula 1 and of Formula 2, and pharmaceutical carriers suitable for the route of administration. Standard methods for formulating pharmaceutical compositions of this type may be found in Remington's Pharmaceutical Sciences. Mack Publishing Company, Easton, PA.
For topical administration, the pharmaceutical composition may be in the form of a salve, cream, ointment, spray, powder or the like. Standard pharmaceutical carriers for such compositions may be used. Preferably, compositions for topical administration will contain 0.05-5% of the active ingredient.
A typical cream formulation may contain the following:
Ingredient
Parts bv Weight
Water/glycol mixture (15% or more glycol)
50-99
Fatty alcohol Non-ionic surfactant Mineral oil
1-20
0-10
0-10
40020PCT
16791
Typical pharmaceutical adjuvants
Active ingredient
0-5
0.05-5
A typical ointment formulation may contain the following:
Ingredients Parts bv Weight
White petrolatum 40-94
Mineral oil 5-20
Glycol solvent 1-15
Surfactant 0-10
Stabilizer 0-10
Active ingredient 0.05-5
For oral administration, suitable pharmaceutical carriers include mannitol, lactose, starch, magnesium stearate, talcum, glucose and magnesium carbonate. Oral compositions may be in the form of tablets, capsules, powders, solutions, suspensions, sustained release formulations, and the like.
A typical tablet or capsule may contain the following:
Ingredients Percent w/w
Lactose, spray-dried Magnesium stearate Cornstarch Active ingredient
40-99
1-2
-20
0.001-20
Parenteral compositions are prepared in conventional suspension or solution forms, as emulsions or as solid forms for reconstruction. Suitable carriers are water, saline, dextrose, Hank's solution, Ringer's solution, glycerol, and the like. Parenteral administration is usually by injection which may be subcutaneous, intramuscular or intravenous.
Έ911267
40020PCT
16791
The compounds of this invention may be combined with other known anti-inflammatory/immunosuppressive agents such as steroids or non-steroidal anti-inflammatory agents (NSAID) in the pharmaceutical compositions and methods described herein.
The assay procedures by which useful biological activity of the compounds of the invention can be demonstrated, are described below.
Calcium Channel (mobilization) Inhibition Assay 10 Polymorphonuclear leukocytes (PMNa), gastric glands,
GH3 cells, A431 cells, spleen cells, human keratinocytes corneal cells, etc. were loaded with the Ca2+ sensitive fluorescent dye, Fura-2. The appropriate cell type was chosen and the potency and efficacy of the anti15 inflammatory furanones on calcium mobilization, calcium channel inhibition was quantitated. The methods used for A431 cells listed below are representative of those used for other cells.
A431 cells were detached using a 5-10 min trypsin20 EDTA treatment whereas GH3 cells were treated 2 to 5 min with a It pancreatin solution. Cells were immediately washed twice in a 20mM HEPES buffer (pH 7.4) containing 120mM NaCl, 6 mM KCl, 1 mM MgSO*, 1 mg/ml glucose and 1 mg/ml pyruvate and 1.4mM calcium (medium A).
Approximately 5 x 106 cells were suspended in medium A and incubated with 4uM fura-2-AM for 15 min at 37°C.
After washing the fura-2 loaded cells, the uptake of dye was checked using fluorescence microscopy and found to be evenly distributed in the cytosol of all cells.
Fluorescence was continuously recorded with a Perkin-Elmer LS-5 spectrofluorometer. The excitation wavelength was set at 340nm and emission wavelength set at 500nm. The cell suspension was continually stirred, maintained at
40020PCT
16791
7°C and equilibrated for approximately 5 min before addition of various agents. [Ca2+i was calculated using the following formula:
[Ca2+]x - 220 X F ~
Fmax - F
All fluorescence values were measured relative to a EGTA-quenched signal determined as follows: F was the relative fluorescence measurement of the sample. Fmax was determined by lysing the cells with digitonin (lOOug/ml) in DMSO. After Fmax was determined the pH was adjusted to 8, with NaOH and Ca2+ chelated with 3mM EGTA to totally quench the fura-2 signal and obtain FfflXn.
When quin-2- was used, cells were incubated with lOuM quin-2- at 37°C for 1 hour, washed and then used.
Mouse Ear Anti-Inflammatorv Assay
Test compound and phorbol myristate acetate (PMA) are topically applied simultaneously to the pinnae of the left ears of mice. PMA alone is applied to the right ear.
Three hours and 20 minutes after application, the mice are sacrificed, left and right ears removed, and standard sized bores taken. Edema (inflammation) is measured as the difference in weight between left and right ears [Van Arman, C.G., Clin Pharmacol Ther (1974) 16:900-904]. Inhibition of Phospholipase A2
The effect of compounds of this invention on bee venom phospholipase A2 determined by the following procedure:
a. Bee venom phospholipase λ2 in 10 uM HEPES (pH
7.4) with 1 mM CaCl2 is incubated with vehicle or test agent for 1.0 hour at 41°.
b. 1.36 mM phosphotidylcholine, 2.76 mM Triton XIE 911267
40020PCT
16791
100 are dispersed in buffer by sonication and then mixed with L-3 phosphotidylcholine, 1-palmitoyl
2(1-14C) palmitoyl for 10 min.
c. Start the reaction by the addition of enzyme (0.495 units/ml).
d. Incubation for 15 sec. at 41°.
e. Reaction is terminated by addition of 2.5 ml of isopropanol: n-heptane: 0.5 M H2SO4 (40:10:1;
v:v:v:).
f. 2.0 ml n-heptane and 1.0 ml H20 added; mixture centrifuged.
g. 2.0 ml n-heptane removed and treated with 200300 mg of silica gel HR60.
h. Samples centrifuged; 1 ml of n-heptane SN removed and added to 10 ml scintillation fluid.
i. Samples counted on a scintillation counter. Inhibition of Phosphoinositide-specific Phospholipase C
The effect of compounds of this invention on phosphoinositide-specific phospholipase C may be determined by procedures described by Bennett et al, Molecular Pharmacology 32:587-593 (1987).
Activity Data
In the above-described phospholipase A2 assay the compounds of the invention were found to provide 50% inhibition (IC50) of bee venom phospholipase A2 at the following concentration· (in micromoles), as indicated in Table 1.
40020PCT
16791
IC50 (urn) 0.03 6
0.9
Table 1
Phospholipase A2 Assay.
Compound name or number 5 1*
9 *Data for Compound 1 (monoalide) are provided for comparison.
In the above-described phospholipase A2 assay compounds of the invention which are listed in Table 2 were found to provide the following percentage (%) inhibition of bee venom phospholipase A2 at 100 micromolar concentration.
Tlfelt I
37
49
53
The compounds of the present invention can be made by the synthetic chemical pathways which are illustrated above in general terms, and in the specific examples as well. The synthetic chemist will readily appreciate that the conditions described here in general terms, and specifically, can be generalized to any and all compounds represented by Formula 1 or by Formula 2, as applicable. Furthermore, the synthetic chemist will readily appreciate that the herein described synthetic steps may be varied or adjusted by those skilled in the art without departing from the scope and spirit of the invention. Therefore, the following examples of specific compounds of the invention, and specific examples of the synthetic steps in which the compounds and certain intermediates are made,
40020PCT
16791 are set out to illustrate the invention, not to limit its scope.
Specific Examples
Example.-1
Ethvl 4-hvdroxv-6-phenylhex-l-ynoate (Compound ll) n-Butyl lithium (a 1.6 M solution in hexane; 6.7 ml,
.7 mmol) was added dropwise to a solution of ethyl propiolate (1.04 g, 10.6 mmol) in tetrahydrofuran (10 ml) at -78° under argon. After 10 minutes, a solution of hydrocinnamaldehyde (1.42 g, 10.6 mmol) in tetrahydrofuran (5 ml) was added. Stirring was continued at -78°C for 2 hours and acetic acid (1 ml) was added. On warming up to 0°, the reaction mixture was poured into water.
Extraction (ethyl ether) and evaporation of the dried (magnesium sulphate) extracts afforded an oil, which was flash chromatographed with silica using 30% ethyl ether/petroleum ether. Fractions with Rf of about 0.28 on evaporation afforded the title ester as a light yellow
oil.1 NMR (CDC13) 1.34 (m, 3H), 2.10 (m, 2H), 2.40 (br, 1H), 2.83 (t, 2H, J - 8.3 H2), 4.25 (q, 2H, J » 7.3 Hz), 4.50 (t, 1H, J » 7.0 Hz) and 7.29 (m, 5H). LRMS m/e (% abundance) 233 (M++1, 2), 232 (M+, 7),
186 (24), 185 (42), 170 (51), 169 (30), 158 (24), 142 (37), 141 (100) and 105 (84).
-(2-Phenviethvl)-2(5H)-furanone (Compound 12)
A solution of ethyl 4-hydroxy-6-phenylhex-l-ynoate (Compound 11, 585 mg, 2.5 mmol) in ether (12 ml) was 30 hydrogenated over Lindlar catalyst (50 mg) at room temperature for 3 hours. The mixture was filtered through celite and the filtrate was refluxed with 2M hydrochloric acid (1 ml) for 2-1/2 hours. On cooling, the mixture was
40020PCT
16791 dried (magnesium sulphate) and evaporated to dryness to give an oil, which was purified by preparative thin layer chromatography (tic, 20x20 cm, 2000u silica plate;
developed with 30% ethyl ether/petroleum ether). The title furanone was obtained as colorless prisms (recrystallized from ether): mp 66-7°.
XH NMR (CDC13) 1.98-2.16 (m, 2H), 2.86 (m, 2H), 5.08 (m, IH), 6.16 (dd, IH, J = 6.0 Hz, 1.6 Hz), 7.36 (m, 5H) and 7.45 (dd, IH, J = 6.0 Hz, 1.6 Hz).
13C NMR (CDC13) 31.3, 34.9, 82.3, 121.5, 126.3,
128.5, 128.6, 140.2, 156.1 and 172.9.
HRMS exact mass calculated for c12h12°2 188.0837, found 188.0841.
Exampl£...2
4-Hvdroxv-6-phenvlhex-2-ynoic acid (Compound 13)
A solution of potassium hydroxide (377 mg, 6.7 mmol) in 95% ethanol (10 ml) was added to a solution of ethyl 4 hydroxy-6-phenylhex-l-ynoate (Compound 11, 1.04 g, 4.5 mmol) in the same solvent (10 ml) at 0°, and the reaction mixture was stirred at room temperature for 15 hours. After most of the solvent was removed, the residue was dissolved in water (ca. 15 ml) and extracted with dichloromethane (discarded). After the extraction the aqueous phase was acidified to pH 1 with dilute hydrochloric acid and extracted thoroughly with ethyl acetate. Evaporation of the dried (magnesium sulphate) extracts gave the title acid as a pale yellow oil (which crystallizes slowly on standing), which was used directly in the next step.
XH NMR (CDC13) 2.16 (Β, 2H), 2.85 (m, 2H), 4.52 (dd,
IH, J » 11.3 HZ, 6.6 HZ), 5.10 (br, 2H) and 7.31 (m, 5H).
LRMS m/e (% abundance) 204 (M+, 6), 142 (42), 141 (75), 134 (21), 133 (11), 131, (10), 118 (34), 117 (32),
40020PCT
16791
115 (21) and 105 (100).
4- Keto-6-phenvlhex-2-ynoic acid (Compound 14)
A solution of Jones Reagent (a 2.67 M solution in sulphuric acid; 2.07 ml, 5.5 mmol) was added dropwise to a solution of 4-hydroxy-6-phenylhex-2-ynoic acid (Compound 13, 750 mg, 3.7 mmol) in acetone (12 ml) at 0° and the reaction mixture was maintained at 0° for 70 minutes. The mixture was quenched with ethanol (2 ml) and extracted with ethyl ether. Evaporation of the dried (magnesium sulphate) extracts gave the title acid as a yellow oil which was used directly in the next step.
1 NMR (CDC13) 3.02 (s, 4H), 7.30 (m, 5H) and 8.80 (br, IH, exchanged with D20).
- Hvdroxv-5-f2-phenvlethvl)-2-furanone (Compound 15)
A solution of 4-keto-6-phenylhex-2-ynoic acid (Compound 14, 228 mg, 1.1 mmol) in ethyl ether (8 ml) was hydrogenated over Lindlar catalyst (20 mg) at 0° for 80 minutes. The mixture was filtered through celite and the filtrate, after evaporation to dryness, was purified by preparative tic (20x20 cm, 1000U silica plate; developed with 60% ethyl ether/hexane). The title furanone was obtained as a colorless oil.
hi NMR (CDC13) 2.31 (dd, 2H, J - 10.8 Hz, 5.5 Hz), 2.78 (dd, 2H, J - 10.8 Hz, 5.5 Hz), 4.80 (br, IH), 6.11 (d, IH, J - 5.8 HZ), 7.20 (m, 5H) and 7.28 (d, IH, J - 5.8
Hz) .
13C NMR (CDC13) 29.7, 39.1, 107.8, 123.1, 126.4, 128.3, 128.7, 140.3, 154.3 and 171.0.
HRMS exact mass calculated for C12H12°3
204.0786, found 204.0792.
Example 3
Ethvl 4-hvdroxvtridec-2-vnoate (Compound 16)
Methylmagnesium bromide (a 3M solution in
40020PCT
16791 tetrahydrofuran; 7.8 ml, 23.4 mmol) vas added dropwise to a solution of ethyl propiolate (Compound 4, 2.25 g, 22.9 mmol) in tetrahydrofuran (10 ml) at -78° under argon.
After 10 minutes, a solution of decyl aldehyde (3.58 g,
22.9 mmol) in tetrahydrofuran (2 ml) vas added. Stirring was continued for 1 hour while the cooling bath was warmed to room temperature. The mixture was quenched with saturated ammonium chloride solution and extracted with ethyl ether. Evaporation of the dried (magnesium sulphate) extracts gave an oil, which was flash chromatographed on silica using 30% ethyl ether/petroleum ether. Fractions with Rf of about 0.31 gave, after evaporation, the title ester as a deep, yellow oil.
XH NMR (CDC13) 0.88 (t, 3H, J - 6.4 Hz), 1.27 (br s,
14H), 1.75 (m, 2H) and 4.25 (q, 2H, J - 6.4 Hz).
LRMS m/e % abundance) 255 (M++l, 5), 254 (M+, 5), 237 (6), 209 (8), 181 (12), 179 (11), 163 (13), 152 (12), 151 (13), 137 (16), 130 (19), 128 (100), 100 (66) and 71 (35).
-Nonvl-2(5H)-furanone (Compound 6)
A solution of ethyl 4-hydroxytridec-2-ynoate (Compound 16, 230.6 ml, 1.02 mmol) in ether (10 ml) was hydrogenated over Lindlar catalyst (20 mg) at 0° for 1 hour. The mixture was filtered through celite and after evaporation the filtrate gave a residue, which was flash chromatographed on silica using 60% ethyl ether/petroleum ether. Fractions with Rf of about 0.18 gave after evaporation a colorless oil (157 mg, 59%) identified by 3H NMR as ethyl (fi)-4-hydroxytridec-2-enoate: 1H NMR (CDC13) 0.92 (t, 3H, J - 6.7 Hz), 1.31 (br S, 14H), 1.65 (m, 2H),
4.97 (q, 1H, J 6.2 Hz), 5.91 (d, 1H, J - 12.5 Hz) and 6.41 (dd, 1H, J - 12.5 Hz, 7.3 Hz). The oil on crystallization from petroleum ether, in the presence of a drop of acetic acid, lactonized to give the title furanone
40020PCT 27 16791 as a colorless oil.
NMR (CDC13) 0.92 (t, 3H, J - 6.7 Hz), 1.30 (br s, 12H), 1.45 (m, 2H), 1.75 (m, 2H), 5.08 (m, IH), 6.14 (dd, IH, J = 5.9 Hz, 2.6 Hz) and 7.48 (dd, IH, J - 5.3 Hz, 1.4 5 Hz) .
13C NMR (CDC13) 14.1, 22.6, 25.0, 29.2, 29.3, 29.4,
31.8, 33.2, 83.4, 121.5, 156.3 and 173.1.
HRMS m/e: exact mass calculated for C13H22°2 <m+> 210.1620, found 210.1624.
io Example..-!
4-Hvdroxvtridec-2-vnoic acid (Compound 17)
A solution of potassium hydroxide (885 mg, 15.8 mmol) in 95% ethanol (35 ml) was added to ethyl 4-hydroxytridec2-ynoate (Compound 16, 2.68 g, 10.5 mmol) in the same solvent (5 ml) at 0°. After stirring at room temperature for 9 hours, most of the solvent was removed and water (20 ml) was added. The mixture was extracted thoroughy with dichloromethane (discarded), acidified to pH 1 with dilute hydrochloric acid and extracted with ethyl acetate.
Evaporation of the dried (magnesium sulphate) ethyl acetate extracts gave an off-white solid, which on recrystallization from petroleum ether (at -78°) gave the title acid as colorless prisms: mp 65-6°.
XH NMR (CDC13) 0.93 (t, 3H, J - 5.7 Hz), 1.31 (br s,
12H), 1.50 (br, IH), 1.81 (m, 2H), 4.56 (dt, IH, J - 5.0
Hz, 1.9 Hz) and 5.00 (br, IH).
LRMS m/e (% abundance) 226 (M+, 5), 137 (13), 124 (12), 121 (18), 107 (26), 100 (94), 97 (33), 95 (27), 93 (43) 85 (48), 83 (63), 79 (55) and 71 (75).
4-Ketotrldec-2-vnoic-acid (Compound 18)
Jones reagent (a 2.67 M solution in sulphuric acid; 1.32 ml, 3.5 mmol) was added dropwise to a solution of 4hydroxytridec-2-ynoic acid (Compound 17, 531.8 mg, 2.4
40020PCT 28 16791 mmol) in acetone (10 ml) at 0° and the reaction mixture was maintained at 0° for 70 minutes. The mixture was quenched with ethanol (1 ml) and dried with magnesium sulphate. On evaporation, the title acid was obtained as 5 a colorless oil.
XH NMR (CDC13) 0.87 (t, 3H, J » 6.6 Hz), 1.25 (br S, 12H), 1.67 (m, 2H), 2.64 (t, 2H, J - 7.2 Hz) and 4.90 (br, IH) .
LRMS m/e (% abundance) 224 (M+, 5), 223 (12), 197 10 (36), 155 (37), 149 (14), 37 (21), 123 (15), 111 (14), 109 (12) and 97 (34) .
-Hvdroxv-5-nonvl-2-furanone (Compound 7)
A solution of 4-ketotridec-2-ynoic acid (Compound 18, 220 mg, 0.98 mmol) in ether (10 ml) was hydrogenated over 15 Lindlar catalyst (10 mg) at 0° for 80 minutes. The mixture was filtered through celite and after evaporation to dryness the filtrate gave an oil, which was purified by preparative tic (20x20 cm, 1000U silica plate; developed with 60% ethyl ether/petroleum ether). The title furanone was obtained as colorless prisms (recrystallized from petroleum ether); mp 54-5°.
ΣΗ NMR (CDC13) 0.88 (t, 3H, J - 7.5 Hz), 1.26 (br s, 12H), 1.40 (m, 2H), 1.98 (m, 2H), 6.12 (d, IH, J - 6.4 Hz) and 7.16 (d, IH, J - 6.4 Hz).
13C NMR (COC13) 14.1, 22.6, 23.4, 29.3, 29.4, 31.8,
37.5, 108.5, 123.0, 154.5 and 170.8.
HRMS m/e: exact mass calculated for c13h22°3 226.1569, found 226.1568.
Example 5
-Hvdroxv-5-aethyl-4-octvl-2-furanone (Compound 19)
A mixture of 2-undecanone (10 g, 58.7 mmol), glyoxylic acid monohydrate (Compound 5, 5.15 g, 56 mmol) and 85% phosphoric acid (10 ml) was warmed at 80° for 18
40020PCT
16791 hours. On cooling to room temperature, the mixture was diluted with ethyl ether/dichloromethane (50 ml each) and washed thoroughly with brine. Evaporation of the dried (magnesium sulphate) organic phase gave a yellow oil which on crystallization from ethyl ether/petroleum ether gave
4- ketotridec-2-enoic acid as colorless prisms.
3H NMR (CDC13) 0.96 (t, 3H, J - 7.4 Hz), 1.34 (br s, 12H), 1.72 (ρ, 2H, J « 7.1 Hz), 2.73 (t, 2H, J = 7.1 Hz), 6.74 (d, 1H, J - 15.7 Hz) and 7.22 (d, 1H, J » 15.7 Hz).
The mother liquor from the above recrystallization was concentrated down and was flash chromatographed on silica using 40% ethyl acetate/petroleum ether. Fractions with Rf of about 0.1 gave, after evaporation, the title furanone as a pale yellow oil.
ΧΗ NMR (CDC13) 0.92 (t, 3H, J - 7.4 Hz), 1.30 (br s,
12H), 2.45 (s, 3H), 2.79 (t, 2H, J - 7.5 Hz) and 6.56 (s, 1H) .
13C NMR (CDC13) 14.1, 22.7, 26.7, 27.0, 29.2, 29.3,
29.8, 31.9, 124.5, 158.0, 171.3 and 200.0.
HRMS m/e: exact mass calculated for C13H22O3 (M+)
226.1569, found 226.1559.
Example 6
- Methvl-4-octyl-2(5H)-furanone (Compound 20)
Sodium borohydride (214 mg, 5.7 mmol) was added to a solution of 5-hydroxy-5-methyl-4-octyl-2-furanone (Compound 19, 640 mg, 2.8 mmol) in tetrahydrofuran (15 ml). After stirring at room temperature for 80 minutes, most of the solvent was removed and water (10 ml) was added. Extraction (dichloromethane) and evaporation of the dried (magnesium sulphate) extracts gave a residue, which was flash chromatographed on silica using 60% ethyl ether/petroleum ether. Fractions with Rf of about 0.23 were evaporated to yield the title furanone as a colorless
40020PCT 30 16791 oil, which crystallized slowly on storage at -70°.
XH NMR (CDC13) 0.85 (t, 3H, J - 5.4 Hz), 1.26 (br s, 10H), 1.33 (d, 3H, J - 6.9 Hz), 1.47 (tt, 2H), 2.26 (tt,
1H), 2.83 (tt, 1H0, 4.33 (q, 1H, J - 6.2 Hz) and 5.99 (s,
1H) .
13C NMR (CDC13) 14.1, 22.4, 22.6, 29.2, 29.3, 29.5,
29.8, 31.9, 71.1, 113.0, 169.2 and 171.8.
HRMS m/e exact mass calculated for C13H22°2 (M+) 210.1620, found 210.1617.
Example..-?·
4-Ethvl-5-hvdroxv-5-niethvl-2-furanone (Comound 21)
A mixture of 2-pentanone (17.1 g, 198 mmol), glyoxylic acid monohydrate (Compound 5, 8.05 g, 88 mmol) and about 85% phosphoric acid (12 ml) was warmed at ca.
80° for 19 hours. On cooling to room temperature the mixture was diluted with ethyl ether/dichloromethane (100 ml, 1:1) and washed thoroughly with brine. Evaporation of the dried (magnesium sulphate) organic phase gave a yellow viscous oil, which on crystallization from ethyl ether/petroleum ether gave 4-keto-hept-2-enoic acid as colorless prisms: mp 100-2°.
XH NMR (CDC13) 1.01 (t, 3H, J - 7.9 Hz), 1.73 (ρ, 2H, J » 7.1. Hz), 2.70 (t, 2H, J - 7.3 Hz), 6.73 (1H, d, J =
.8 Hz) and 7.19 (d, 1H, J - 15.8 Hz, .
HRMS m/e: exact mass calculated for C7H10O3 (M+)
142.0630, found 142.0622.
The mother liquor from the above crystallization was evaporated to dryness and extracted thoroughly with 30 petroleum ether. The combined extracts were concentrated and cooled to -20° to give the title furanone as colorless prisms: mp 37-8°.
XH NMR (CDC13) 1.09 (t, 3H, J 7.8 Hz), 2.46 (s,
40020PCT 31 16791
3H) , 2.83 (q, 2H), J - 7.8 Hz) and 6.57 (s, IH).
13C NMR (CDC13) 13.6, 20.4, 26.6, 124.6, 158.7, 171.2 and 199.9.
HRMS m/e: exact mass calculated for C7H10O3(M+) 5 142.0630, found 142.0622.
Example. S
4-Ethyl-5-methvl-2(5H)-furanone (Compound 22)
Sodium borohydride (646 mg, 17 mmol) was added to a solution of 4-ethyl-5-hydroxy-5-methyl-2-furanone (Compound 21, 1.21 g, 8.5 mmol) in tetrahydrofuran (10 ml) at room temperature. After 1/2 hour, most of the solvent was removed and water (10 ml) was added. Extraction (ethyl acetate) and evaporation of the dried (magnesium sulphate) extracts gave an oil, which was flash 15 chromatographed on silica using 60% ethyl ether/petroleum ether. Fractions with Rf of about 0.23 gave after evaporation, a pale yellow oil, which slowly crystallized on storage at -20°. Recrystallization from ethyl ether/petroleum ether afforded the title furanone as colorless prisms: mp 86-7°.
XH NMR (CDC13) 1.16 (t, 3H, J - 7.2 Hz), 1.39 (d, 3H,
J - 5.4 HZ), 2.36 (m, IH), 2.84 (m, IH), 4.41 (q, 2H, J 7.2 HZ) and 6.04 (s, IH).
13C NMR (CDC13) 13.8, 22.3, 22.8, 71.0, 112.9, 170.3 and 171.7.
HRMS m/e: exact mass calculated for Ο7Η10Ο2(M+) 126.0681, found 126.0683»
Example 9
3.4-Dimethyl-5-hvdroxv-5-(1-octvnvl)-2-furanone (Compound
23)
D-Butyl lithium (a 1.6 M solution in hexane; 6.78 ml, 10.9 mmol) was added dropwise to a solution of l-octyne (1.13 g, 10 mmol) in tetrahydrofuran (7 ml) at -78° under
40020PCT
16791 argon. After 20 minutes, the solution was cannulated dropwise, under argon, to a solution of 2,3-dimethylmaleic anhydride (1.30 g, 10.3, mmol) in tetrahydrofuran (15 ml) cooled at -78°. Stirring was continued for 2 hours while the cooling bath attained room temperature. The mixture was quenched with dilute hydrochloric acid, diluted with water (10 nl) and extracted with ethyl acetate.
Evaporation of the dried (magnesium sulphate) extracts gave an oil, which was flash chromatographed on silica 10 using 30% ethyl ether/petroleum ether. Fractions with Rf of about 0.18 on evaporation afforded a light yellow viscous oil, which crystallized out slowly on storage at -20°. Recrystallization from petroleum ether gave the title furanone as colorless prisms: mp 55-6°C.
1H NMR (CDC13) 0.85 (t, 3H, J - 7.4 Hz), 1.24 (m,
6H) , 1.49 (ρ, 2H, J - 7.9 Hz), 1.79 (s, 3H), 2.00 (β, 3H), 2.21 (t, 2H, J - 7.2 HZ) and 3.93 (br, 1H).
13C NMR (CDC13) 8.4, 10.5, 13.9, 18.5, 22.4, 27.9, 28.4, 31.1, 74.5, 88.2, 98.0, 124.3, 156.9 and 172.1.
HRMS m/e: exact mass calculated for C14H20°3
237.1491, found 237.1498.
Example 10
3.4-Dimethyl-5-hydroxv-5-(2-phenvlpropvl)-2-furanone (Compound 24) a mixture of 3-phenyl-l-bromopropane (521 mg, 2.6 mmol) and magnesium turnings (66 mg, 2.8 mmol) in tetrahydrofuran (5 ml) was refluxed under argon for 90 minutes. After the reaction mixture had been cooled to -78°, a solution of 2,3-dimethylmaleic anhydride (330 mg,
2.6 mmol) in tetrahydrofuran (5 ml) was added dropwise.
Stirring vas continued overnight (ca. 17 hours) while the cooling bath attained room temperature. The mixture was quenched with a saturated solution of ammonium chloride
40020PCT
16791 and extracted with ethyl acetate. Evaporation of the dried (magnesium sulphate) extracts gave an oil, which was flash chromatographed on silica using 40% ethyl acetate/petroleum ether. Fractions with Rf of about 0.32 on evaporation afforded the title furanone as a pale yellow oil, which on storage at -20° crystallized as colorless prisms: mp 62-3°C.
1H NMR (CDC13) 1.48 (m, IH), 1.70 (m, IH), 1.79 (s, 3H), 1.89 (s, 3H), 2.05 (m, 2H), 2.65 (m, 2H) and 7.25 (m,
5H) .
13C NMR (CDC13) 8.3, 10.6, 24.7, 35.4, 107.2, 125.1, 125.9, 128.4, 141.5, 158.1 and 172.6.
HRMS m/e: exact mass calculated for C15H18°3 <M+> 246.1256, found 246.1270.
Example 11
3.4-Dlmethvl-5-(2-phenvlpropvl)-2(5H)-furanone (Compound 8)
Potassium borohydride (503 mg, 9.3 mmol) was added to a solution of 3,4-dimethyl-5-hydroxy-5-(2-phenylpropyl)-220 furanone (Compound 24, 382 mg, 1.6 mmol) in tetrahydrofuran (8 ml) and methanol (6 ml) at room temperature. After 7 hours, most of the solvent was removed and water (10 ml) was added. Extraction (ethyl acetate) and evaporation of the dried (magnesium sulphate) extracts gave an oil, which was purified by preparative tic (20x20 cm, 2000u silica plate; developed with 30% ethyl ether/petroleum ether). The title furanone was obtained as colorless prisms (recrystallized from ethyl ether/petroleum ether): mp 69-70°.
3H NMR (CDC13) 1.50 (m, IH), 1.77 (ρ, 2H, J - 6.8
HZ), 1.82 (s, 3H), 1.92 (a, 3H), 1.95 (m, IH), 2.67 (m,
2H), 4.74 (m, IH) and 7.25 (m, 5H).
13C NMR (CDC13) 8.4, 11.9, 26.0, 31.5, 35.4, 82.9,
40020PCT 34 16791
123.6, 125.9, 128.4, 141.5 and 158.9.
HRMS m/e: exact mass calculated for C15H18°2 <M+>
230.1307, found 230.1311.
Example 12
4-Qctvl-5-hydroxY-2(5H)-furanone (Compound 25)
A mixture of glyoxylic acid monohydrate (Compound 5,
1.19 g, 16.1 mmol), morpholine hydrochloride (1.81 g, 14.6 mmol), water (0.73 ml) and 1-decanal (2.89 ml, 15.4 mmol) in dioxane (6 ml) was stirred at room temperature for 1 hour, followed by reflux for 25 hours. After cooling, most of the solvent was removed by evaporation and the residue was extracted with ethyl ether. Evaporation of the dried (magnesium sulfate) extracts gave an oil, which was flash chromatographed with 30% ethyl acetate/hexane to give the title furanone.
XH NMR (CDC13): 0.89 (t, 3H, J - 6.6 Hz), 1.25 (br s,
10H), 1.60 (m, 2H), 2.40 (m, 2H), 4.70 (br, 1H), 5.84 (s, 1H) and 6.00 (s, 1H).
4-Qctvl-5-methoxv-2 f5H)-furanone (Compound 26)
A mixture of 4-octyl-5-hydroxy-2(5H)-furanone (Compound 25, 244 mg, 1.16 mmol) and l-toluenesulfonic acid (33 mg, 0.17 mmol) and methanol (5.8 ml) was stirred at room temperature for 2 days. The mixture was diluted with ethyl ether and washed thoroughly with 5% sodium bicarbonate solution. Evaporation of the dried (magnesium sulfate) organic phase gave an oil, which was flash chromatographed on silica using 10% ethyl acetate/hexane to give the title furanone.
ΤΗ NMR (CDC13): 0.89 (t, 3H, J - 7.5 Hz), 1.28 (br s,
10H), 1.60 (m, 2H), 2.35 (m, 2H), 3.56 (s, 3H), 5.64 (s,
1H) and 5.86 (s 1H).
3-Bromo-4-octvl-5-methoxy-2(5H)-furanone (Compound 27)
A solution of bromine (28 microliter) in
40020PCT
16791 carbontetrachloride (0.2 ml) vas added to a solution of 4octyl-5-methoxy-2(5H)-furanone (100 mg, 0.45 mmol) in carbon tetrachloride (0.5 ml) at 0°. The mixture was stirred at room temperature until all the starting material disappeared (as monitored by tic). After cooling to 0°, pyridine (86 microliter, 1.17 mmol) was added. The mixture was quenched with water and the layers were separated. Evaporation of the dried (magnesium sulfate) organic phase gave an oil, which was purified by flash chromatography using 5% ethyl acetate/hexane to give the title furanone.
1H NMR (CDC13): 0.89 (t, 3H, J - 6.8 Hz), 1.28 (br s, 15H), 1.60 (m, 2H), 2.50 (m, 2H), 3.58 (s, 3H) and 5.69 (s, IH).
3-Bromo-4-octvl-5-hvdroxv-2(5H)-furanone (Compound 9)
A mixture of 3-bromo-4-octyl-5-methoxy-2(5H)-furanone (106 mg, 0.35 mmol) and concentrated hydrochloric acid (0.21 ml) was refluxed until all the starting material disappeared as shown by tic. After cooling, the mixture was diluted with ethyl ether and vas neutralized by washing thoroughly with saturated potassium bicarbonate solution.. Evaporation of the dried (magnesium sulfate) organic phase gave an oil, which was flash chromatographed on silica using 10% ethyl acetate/hexane to give the title furanone.
IR (CHC13): 3389, 1755 and 1651.
XH NMR (CDC13): 0.87 (t, 3H, J - 7.2 Hz), 1.27 (br s, 10H), 1.60 (Β, 2H), 2.49 (t, 2K, J - 8.5 Hz), 4.50 (br,
IH), and 6.05 (s, IH).
13C NMR (CDC13): 14.2, 22.7, 26.5, 27.8, 29.2, 29.6,
31.8, 98.8, 112.1 and 163.9.
HRMS exact mass calculated for c12H20BrO3 <M+H)+ 291.0596, found 291.0590.
Claims (43)
1. Compounds of the formula wherein R x is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds, or arylalkyne having one or more triple bonds; R 2 is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds or arylalkyne having one or more triple bonds, and R 3 is H, alkyl of 1 to 20 carbons, arylalkyl, or halogene.
2. Compounds of Claim 1 wherein R^ is hydrogen, arylalkyl or long chain alkyl.
3. Compounds of Claim 2 wherein R 3 is long chain nenial alkyl.
4. Compounds of Claim 2 wherein R^ is 3-aryl-npropyl.
5. alkyl. Compounds of Claim 1 wherein R 2 is hydrogen or alkyl. Compounds Compounds of Claim 5 wherein R 2 of Claim 5 wherein R 2 is lower alkyl, is long chain
6.
7. 40020PCT 37 16791
8. Compounds of Claim 1 wherein R 3 is H, or lower alkyl.
9. Compounds of Claim 1 wherein R 3 is bromo.
10. One or more compounds set forth in Claim 1, comprised in and admixed with a pharmaceutical composition including a pharmaceutically acceptable excipient.
11. Compounds of the formula Αυ wherein R x is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds, or arylalkyne having one or more triple bonds; 15 R 3 is H, alkyl of 1 to 20 carbons, alkylene having one or more double bonds, alkyne having one or more triple bonds, arylalkyl, arylalkylene having one or more double bonds or arylalkyne having one or more triple bonds; R 3 is H, alkyl of 1 to 20 carbons, arylalkyl, or 20 halogene, and X is H or alkyl of 1 to 20 carbons, CO-X*, CO-O-X*,, CO-NH-X*,, or PO(OX*,) 2 or PO(OX*, )X*,, where X*, independently is H, alkyl of 1 to 20 carbons, phenyl, or substituted phenyl, with the proviso that R x and R 3 25 both are not hydrogen.
12. Compounds of Claim 11 wherein R x is hydrogen, 40020PCT 16791 arylalkyl or long chain alkyl.
13. Compounds of Claim 12 wherein R x is long chain normal alkyl.
14. Compounds of Claim 12 wherein R x is 3-aryl-n- propyl.
15. Compounds of Claim 11 wherein R? is hydrogen or alkyl.
16. Compounds of Claim 15 wherein R? is lower alkyl.
17. Compounds of Claim 15 wherein R 2 is long chain alkyl.
18. Compounds of Claim 11 wherein R 3 is H, or lower alkyl.
19. Compounds of Claim 11 wherein R 3 is bromo.
20. Compounds of Claim 11 wherein X is hydrogen, CH 3 , or ch 3 co.
21. One or more compounds set forth in Claim 11, comprised in and admixed with a pharmaceutical composition including a pharmaceutically acceptable excipient.
22. Compounds of the formula wherein R x is H, alkyl of 1 to 20 carbons, or arylalkyl; R 2 is H, or alkyl of 1 to 20 carbons, and 40020PCT 39 16791 Rj is H, alkyl of 1 to 20 carbons, or halogene.
23. Compounds of Claim 22 wherein Rj is n-nonyl.
24. Compounds of Claim 23 wherein R 2 is hydrogen.
25. The compound of Claim 24 wherein Rj is hydrogen
26. Compounds of Claim 22 wherein Rj is (CH 2 ) 3 -C 6 H 5
27. Compounds of Claim 26 wherein R 2 is methyl.
28. The compound of Claim 27 wherein Rj is methyl.
29. Compounds of Claim 26 wherein Rj is methyl.
30. Compounds of Claim 22 wherein Rj is hydrogen.
31. Compounds of Claim 30 wherein R 2 is n-octyl.
32. Compounds of Claim 30 wherein Rj is hydrogen.
33. The compound of Claim 31 wherein Rj is hydrogen
34. Compounds of the formula 10 wherein R 2 is H, alkyl of 1 to 20 carbons, or arylalkyl; R 2 is H, or alkyl of 1 to 20 carbons; Rj is H, alkyl of 1 to 20 carbons, or halogene, and Z is H or alkyl of 1 to 20 carbons with the proviso 13. 15 that Rj and Rj both are not hydrogen.
35. Compounds of Claim 34 wherein Rj is n-nonyl.
36. Compounds of Claim 35 wherein R 2 is hydrogen.
37. Compounds of Claim 36 wherein Rj is hydrogen.
38. The compound of Claim 37 wherein Z is hydrogen.
39. Compounds of Claim 34 wherein Rj is bromine. 40020PCT 16791
40. Compounds of Claim 39 wherein Rj is n-octyl.
41. Compounds of Claim 40 wherein R x is hydrogen.
42. The compound of Claim 41 wherein X is hydrogen.
43. A process for the preparation of a compound as claimed in any of the preceding claims, substantially as hereinbefore described by way of Example.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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US51036490A | 1990-04-17 | 1990-04-17 |
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IE911267A1 true IE911267A1 (en) | 1991-10-23 |
Family
ID=24030446
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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IE126791A IE911267A1 (en) | 1990-04-17 | 1991-04-16 | 2(5h)-furanones substituted in the 5 and or in the 4¹position, as anti-inflammatory agents |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0524979A4 (en) |
JP (1) | JPH05506215A (en) |
AU (1) | AU7559691A (en) |
CA (1) | CA2078771A1 (en) |
IE (1) | IE911267A1 (en) |
WO (1) | WO1991016055A1 (en) |
Families Citing this family (18)
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US5268387A (en) * | 1992-04-24 | 1993-12-07 | Allergan, Inc. | Pharmaceutical compositions and method for administering 3 and 4-substituted 2(5H)-furanones to a mammal for inhibiting bone loss |
US6492413B2 (en) | 1993-01-15 | 2002-12-10 | G.D. Searle & Co. | 3.4-diaryl thiophenes and analogs thereof having use as antiinflammatory agents |
US5474995A (en) | 1993-06-24 | 1995-12-12 | Merck Frosst Canada, Inc. | Phenyl heterocycles as cox-2 inhibitors |
GB9602877D0 (en) * | 1996-02-13 | 1996-04-10 | Merck Frosst Canada Inc | 3,4-Diaryl-2-hydroxy-2,5- dihydrofurans as prodrugs to cox-2 inhibitors |
WO1995005376A1 (en) * | 1993-08-19 | 1995-02-23 | Warner-Lambert Company | Substituted 2(5h)furanone, 2(5h)thiophenone and 2(5h)pyrrolone derivatives, their preparation and their use as endothelin antagonists |
JP2788677B2 (en) * | 1994-01-10 | 1998-08-20 | メルク フロスト カナダ インコーポレーテツド | Phenyl heterocycle as COX-2 inhibitor |
US5451686A (en) * | 1994-04-15 | 1995-09-19 | Allergan, Inc. | 3 and 5 alkyl and phenyl 4-(hydroxy or acyloxy)-alkyl substituted 2(5H)-furanones as anti-inflammatory agents |
US5639468A (en) * | 1995-06-07 | 1997-06-17 | University Of Southern California | Method for reducing or preventing post-surgical adhesion formation using manoalide and analogs thereof |
US5922759A (en) * | 1996-06-21 | 1999-07-13 | Warner-Lambert Company | Butenolide endothelin antagonists |
AU6603996A (en) * | 1995-08-24 | 1997-03-19 | Warner-Lambert Company | Furanone endothelin antagonists |
US5981576A (en) * | 1995-10-13 | 1999-11-09 | Merck Frosst Canada, Inc. | (Methylsulfonyl)phenyl-2-(5H)-furanones as COX-2 inhibitors |
US6020343A (en) * | 1995-10-13 | 2000-02-01 | Merck Frosst Canada, Inc. | (Methylsulfonyl)phenyl-2-(5H)-furanones as COX-2 inhibitors |
GB9615867D0 (en) * | 1996-07-03 | 1996-09-11 | Merck & Co Inc | Process of preparing phenyl heterocycles useful as cox-2 inhibitors |
US5891892A (en) * | 1996-10-29 | 1999-04-06 | Warner-Lambert Company | Small molecule biaryl compounds as inhibitors of endothelin converting enzyme |
US6080876A (en) * | 1997-10-29 | 2000-06-27 | Merck & Co., Inc. | Process for making phenyl heterocycles useful as COX-2 inhibitors |
CN101801186A (en) * | 2007-05-04 | 2010-08-11 | 艾克沃制药生物发明有限公司 | Natural bioactive compounds |
FR2978963A1 (en) | 2011-08-11 | 2013-02-15 | Ascorbix | NOVEL DERIVATIVES OF FURANONES AND PHARMACEUTICAL COMPOSITION CONTAINING SAME |
RU2522598C1 (en) * | 2013-03-29 | 2014-07-20 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Кубанский государственный технологический университет" (ФГБОУ ВПО "КубГТУ") | Method of obtaining 2(5h)-furanone |
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US2359208A (en) * | 1941-03-21 | 1944-09-26 | Lilly Co Eli | beta-substituted-delta alpha,beta-gamma-butyrolactones and beta-substituted-beta-hydroxy-gamma-butyrolactones and the methods of preparing them |
US2359096A (en) * | 1941-03-21 | 1944-09-26 | Lilly Co Eli | beta-substituted-delta alpha, beta-gamma-butyrolactones and beta-substituted - beta - hydroxy-gamma-butyrolactones and methods of preparing them |
DE2538771A1 (en) * | 1975-09-01 | 1977-03-17 | Henkel & Cie Gmbh | Antimicrobial agents contg. (2)-furanone derivs. - used e.g. as cleansing agents and disinfectants for textiles, hospital equipment, breweries and bathing pools |
JPS6019902B2 (en) * | 1979-03-28 | 1985-05-18 | 三井東圧化学株式会社 | Dimer of isopropenylphenylmaleimide derivative |
US4874782A (en) * | 1985-07-01 | 1989-10-17 | Eli Lilly And Company | Furanone derivatives |
FR2603036B1 (en) * | 1986-08-22 | 1988-11-25 | Rhone Poulenc Agrochimie | 2,3-DIHYDROFURANNE DERIVATIVES, THEIR PREPARATION PROCESS, THEIR USE AS AN INTERMEDIATE FOR THE PREPARATION OF TETRAHYDROFURANNE |
-
1991
- 1991-03-25 WO PCT/US1991/002005 patent/WO1991016055A1/en not_active Application Discontinuation
- 1991-03-25 CA CA002078771A patent/CA2078771A1/en not_active Abandoned
- 1991-03-25 AU AU75596/91A patent/AU7559691A/en not_active Abandoned
- 1991-03-25 JP JP91506660A patent/JPH05506215A/en active Pending
- 1991-03-25 EP EP19910907029 patent/EP0524979A4/en not_active Ceased
- 1991-04-16 IE IE126791A patent/IE911267A1/en unknown
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JPH05506215A (en) | 1993-09-16 |
WO1991016055A1 (en) | 1991-10-31 |
EP0524979A4 (en) | 1993-03-10 |
EP0524979A1 (en) | 1993-02-03 |
CA2078771A1 (en) | 1991-10-18 |
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