IE48729B1 - Intermediates for the preparation of thienamycin and process for preparing same - Google Patents

Description

This invention relates to compounds of the formula J_NR1 wherein a) R1 is R8 is R9 is b) R1 is R8 is hydrogen or a protecting group, hydrogen and -ch2i, a protecting group.

OH CH R9 and is -CH2C(SR)3 wherein R is selected from lower alkyl having 1-6 carbon atoms, aryl and aralkyl having 6 to 10 carbon atoms, and wherein R can be the same or different, c) R1 is hydrogen, R8 is ^OH -CH and CH.

R9 is -CH2-C-CH2-CO2R7 wherein R7 is a protecting group, d) is hydrogen, R8 is and -CH OH CH9 7 7 R is -CH^-C-C—-CO2R wherein R is a protecting group.

These compounds can be used as intermediates for the total synthesis of the known antibiotic thienamycin (I) OH Λ COOH -NH.

Starting from L-aspartic acid, the synthesis 15 proceeds in a stereo-selective way via intermediates II, III, IV: I NH II ΛΓ& COOR IV 487 29 wherein X is a conventional leaving group, 3uch as halo or organosulfonvloxy, and R is hydrogen, a conventional, readily removable protecting group, such-as an ester, or a salt cation. The details of the total synthesis are given below.

The intermediates of the present invention may conveniently be prepared and used in the process which is summarized by the following reaction diagram: 87 29 OR2 tl? OR^ Λ_-Ζ ‘CHO SR vu2* Λτ—Χγ011 , L- CO,M SR' OR* OR Λ' .

SR' 'C02* OH I In words relative to the above diagram, L-aspartic acid j. is esterified according to well known procedures. Typically 1 in a solvent such as benzene, toluene or chloroform is treated with an esterifying agent such as benzyl alcohol, methanol, ethanol or isopropanol, in the presence of for example p-toluene sulfonic acid, HC1 or HBr at a temperature of from 0 to 110°C for from 1 to 24 hours to achieve the desired establishment and hence protection of the carboxyl functions. The resulting species 2^in a solvent such as ether, THF or DME is treated with for example trimethylchlorosilane followed by treatment with EtMgBr, MeMgl, 0MgBr or t-BuMgCl at a temperature of from -40 to 50°C for from 1 to 72 hours to provide azetidinone^.

Reduction of species J^with a reducing agent such as NaBH^, in a solvent such as methanol, ethanol or isopropanol at a temperature 2o of from -10 to 40“C for from 1 to 6 hours provides _4^ (For purposes here, the symbols: Et, Me, 0, iPr, and t-Bu stand for: ethyl, methyl, phenyl isopropyl, and tert-butyl, respectively.) Treatment of j^in a solvent such as methylene chloride or CHCl3 with for example methane sulfonyl chloride or methane sulfonic anhydride in or the presence of a base such as Et3N^iPr2NEt, followed by treatment with a stoichiometric to fold excess of sodium iodide in acetone yields via 4a.

The transformation 5 _> 6 establishes the protecting group IC which may be a triorganosilyl group, such as t-butyldimethylsilyl, t-butyldiphenylsilyl, triphenylsilyl, isopropyldimethylsilyl, for example,or may be 3,4-dimethoxybenzyl, for example. Silyl protection is preferred, and typically R1 is established by treating in a solvent such as dimethylformamide, acetonitrile, hexamethylphosphoramide or tetrahydrofuran with a silylating agent such as t-butyldimethylchlorosilane, t-butyldiphenylchlorosilane or triphenylchlorosilane at a temperature of from -20’ to 25’C for from 0.5 to 24 hours in the presence of a base such as triethylamine, diisopropylethylamine, or imidazole.

The transformation 6 -t>7 is accomplished by treating £ in a solvent such as tetrahydrofuran, dimethoxyethane or diethylether with a carbanion generically represented by the following structure: ., SR2 M*C® — SR3 SR4 wherein M is a metal cation such as lithium, potassium, copper or magnesium, for example, 3 4 and R , R and R are selected from alkyl, aryl or 25 aralkyl such as methyl, ethyl, benzyl, methoxybenzyl, trityl and phenyl, for example, at a temperature of from -100 to O’C and from 0.5 to 4 hours.

Typically, the carbanion reagent is prepared prior to addition of substrate 6 on treatment of the triorganothiomethane with a strong base such as n-butyllithiuro, t-butyllithium, phenyllithium or lithium diisopropylamide (LDA).

The alkylation^—is accomplished by treating 7 in a solvent such as tetrahydrofuran, dimethoxyethane, diethylether, hexamethylphosphoramide, at a temperature of from -100° to -20°C with a strong base such as lithium diisopropylamide,lithium hexamethyldisilazide, lithium 2,2,6,6-tetramethylpiperidide or potassium hydride followed by the addition of an equivalent to 10 fold excess of acetaldehyde. This reaction gives a mixture of isomers from which the desired trans-R form can be conveniently separated by chromatography or Crystallization The transformation 8 —* 9 is accomplished by treating J3, in a solvent such as methanol, ethanol, isopropanol or water at a temperature 2o of from 0 to 80aC with a Lewis acid such as mercuric chloride, silver tetrafluoroborate or thallium trinitrate. The value of R8 is determined by the identity of the alcohol taken in reaction.

The transformation 9—>10 establishes the hydroxyl zz protecting group Rz. The most preferred protecting groups R2 are triorganosilyl groups such as t-butyldimethylsilyl, t-butyldiphenylsilyl or triphenylsilyl. Typically, silylation is accomplished by treating ^with the corresponding tri30 organosilyl chloride in a solvent such as dimethylformamide, acetonitrile or tetrahydrofuran at a temperature of from -20 to 80eC for from 0.5 to 24 hours.

The reduction 1O_^1J is accomplished by treating 10 in a solvent such as toluene, methylene chloride diethylether or tetrahydrofuran with a reducing agent such as diisobutylaluminum hydride or sodium bis(2-methoxyethoxy)aluminum hydride at a temperature of from -100° to -40°C for from 1 to 10 hours.

The addition 11->12^ is accomplished by treating 11 in a solvent such as tetrahydrofuran, diethylether or dimethoxyethane at a temperature of from -100° to Q’C for from 15 minutes to 2 hours in the presence of LiCHjCOjR6; wherein R6 is benzyl, pmethoxybenzyl or 2,4-dimsthoxybenzyl, for example; which reagent is typically generated in situ on treatment of the appropriate R6 acetate with a strong base such as LDA, lithium hexamethyldisilazide or lithium 2,2,6,6-tetramethylpiperide.

If desired, a more readily removable carboxyl protecting group may conveniently replace the first established group, R6, ly the carboxyl protecting group R . This transformation 12->13^1^_is accomplished by selectively deblocking 12 to form 13^ by hydrogenation or hydrolysis. Typically, the reaction is accomplished by treating 12 in a. solvent such as methanol, ethanol, dioxane, tetrahydrofuran or water under a hydrogenation pressure of from 1 to 4 atmospheres in the presence of a catalyst such as Pd on charcoal or Pd(0H)2 for from 0.1 to 10 hours. The 13, intermediate (M may be H, Na, K or ammonium such as Et^NH, for example) need not be isolated. Intermediate 14 is obtained from the hydrogenation mixture upon treatment with the chosen reagent calculated to establish R7 such as an aralkyl halide In a solvent such as dimethylformamide, acetonitrile, hexamethylphosphoramide at a temperature of from 0° to 50°C for from 0.5 to 18 hours, R7 is typically an aralkyl group such as p-nitrobenzyl, or o-nitrobenzyl, for example.

The oxidation 14—715 is accomplished by treating ii in a solvent such as methylene chloride or acetonitrile with an oxidizing system such as dipyridine chromium (VI) oxide, 3,5-dimethylpyrazole chromium (VI) oxide, pyridinium chlorochromate, pyridinium dichromate, trifluoroacetic anhydride -dimethylsul-foxide or acetic anhydride-dimethyl sulfoxide at a temperature of from -78°C to 25°C for from 5 min. to 8 hrs 2 Removal of protecting groups R and R (15-^16) is accomplished by acidic aqueous hydrolysis of 15 in a solvent such as methanol, ethanol, tetrahydrofuran or dioxane in the presence of an acid such as hydrochloric, sulfuric or acetic acid at a temperature of from 0 to 100’C for from 2 to 18 hours.

The diazo species 17 is prepared from 16 by treating 16 in a solvent such as CH^CN, CH2C12 or THF with an azide such as p-carboxybenzenesulfonylazide, toluenesulfonylazide or methanesulfonylazide in the presence of a base such as tri25 ethylamine, pyridine or (C2H5)2NH for from 1 to 50 hours at 0-25“C.

Cyclization (17—> 18) is accomplished by teating 17 in a solvent such as benzene, toluene or THF at a temperature of from 50-110°C for from 1-5 hours in the presence of a catalyst such as bis (acetylacetonato)Cu (II) [Cu(acac)2J, CuSO^, Cu powder, Rh(OAc)2, or Pd(0Ac)2· Alternatively, the cyclization may be accomplished by irradiating 17, through a pyrex filter (a wave length greater than 300nm) in a solvent such as benzene, CC14 or diethylether at a temperature of from 0-25°C for from 0.5 to 2 hours.[OAc = acetate.) Establishment of leaving group X (18-»19) is accomplished by acylating the keto ester 18, with an acylating agent R*X such as p-toluenesulfonic acid anhydride, p-nitrophenylsulfonio acid anhydride, 2,4,6-triisopropylphenylsulfonic acid anhydride, methanesulfonic acid anhydride, toluenesulfonyl chloride or p-bromophenylsulfonyl chloride wherein X is the corresponding leaving group such as toluene sulfonyloxy, p-nitrophenylsulfonyloxy, methanesulfonyloxy, p-bromophenylsulfonyloxy and other leaving groups which are established by conventional procedures and are well known in the art. Typically, the above acylation to establish leaving groups X is conducted in a solvent such as methylene chloride, acetonitrile or dimethylformamide, in the presence of a base such as diisopropylethylamine, triethylamine or 4-dimethylamino-pyrldine at a temperature of from -20 to 40*c for from 0.5 to 5 hours. The leaving group X of intermediate 19 can also be halogen. The halogen leaving group is established by treating 18, with a halogenating agent such asg-fCljr 0-jPBr2, (0O)3PBr2 or oxalyl chloride in a solvent such as CH2C12, CH^CN or THF in the presence of a base such as diisopropylethylamine, triethylamine or 4-dimethylaminopyridine = phenyl J The reaction 19-»20 is accomplished by treating 19 in a solvent such as dioxane, dimethylformamide, dimethylsulfoxide, acetonitrile or hexamethylphosphoramide in the presence of an approximately equivalent to excess of the mercaptan reagent 8 8 HSCHjCHjNHR wherein R is hydrogen or a readily removable N-protecting group such as p-nitrobenzyloxycarbonyl or o-nitrobenzyloxycarbonyl in the presence of a base such as sodium hydrogen carbonate, potassium carbonate, triethylamine or diisopropylethylamine at a temperature of from -40 to 25°C for from 1 to 72 hours g The mercaptan reagent, HSCHjCHjNHR , is typically prepared by treating aminoethylmercaptan in the presence of a base such as sodium bicarbonate or sodium hydroxide in a solvent such as aqueous diethylether, aqueous dioxane or aqueous acetone at a temperature of from 0 to 25°C for from 0.5 to 4 hours. The reaction of an alkyl sulfide with an alkyl or aryl halide or an arylsulfonate is known from Houben-Weyl, Methoden der Organischen Chemie, 1955, Vol. IX, pages 103 and 114, however, nothing is said in that reference about the reaction with sulfides of organic halides having a lactam ring.

The final deblocking step 20 —»I is accomplished by conventional procedures such as hydrolysis or hydrogenation. Typically 20 in a solvent such as dioxanewater-ethanol or tetrahydrofuran-aqueous dipotassium hydrogen phosphate-isopropanol is treated under a hydrogen pressure of from about 1 to 4 atmospheres in the presence of a hydrogenation catalyst such as palladium on charcoal or palladium hydroxide at a temperature of from 0 to 50°C for from 0.5 to 4 hours to provide I, In the above-discussed aldol reaction J7 —’8, for introduction of the- hydroxyethyl side chain, the scheme proceeds directly to give a mixture of isomers (trans-R, trans-S, and cis R) from which the desired trans-R isomer can be separated ohromatographioally or by crystallization. An indirect aldol reaction scheme stereo-selectively provides the desired trans-R isomer according to the following scheme: Direct Acetylation (D) reduction 4* wherein all symbols are as previously defined In words relative to the above reaction scheme, Step A has been described above. The direct acetylation, Step B,is accomplished by treating 7 with two or more equivalents of a base such as lithium diisopropylamide, lithium hexamethyldisilazide, lithium 2,2,6,6-tetramethyIpiperidide, in a solvent such as tetrahydrofuran, diethylether, or dimethoxyethane, for example, at a temperature of from -100 to -20°C with an acylating agent such as N-acetyl imidazole.

Addition of the / plus base mixture to the acylating agent is preferred.

The oxidation Step C is accomplished with an oxidizing agent such as dipyridine chromium(VI)oxide, trifluoroacetic anhydridepdimethylsulfoxide-triethylamine, pyridinium dichromate, acetic anhydride-dimethylsulfoxide in a solvent such as methylene chloride or acetonitrile, at a temperature of from -78 to 25°C for from 5 minutes to 5 hours.

The reduction Step D is accomplished by contacting the ketone with a reducing agent such as potassium tri(sec - butyl)borohydride, lithium tri(sec-butyl)borohydride,sodium borohydride, sodium tris(methoxyethoxy)aluminum hydride or lithium aluminum hydride in a solvent such as diethylether, tetrahydrofuran or toluene at a temperature of from -20 to 25°C.

The reaction can conveniently be conducted in the presence of an added complexing salt such as potassium iodide or magnesium bromide.

In the foregoing word description of the above schematic reaction diagram for the total synthesis of thienamycin, it is to be understood that there is considerable latitude in selection of precise reaction parameters. Suggestion of this latitude and its breadth is generally indicated by the enumeration of equivalent solvent systems, temperature ranges, protecting groups, and range of identities of involved reagents. Further, it is /to be understood that the presentation of the synthetic scheme as comprising distinct steps in a given sequence is more in the nature of a descriptive convenience than as a necessary requirement; for one will recognize that the mechanically dissected scheme represents a unified scheme of synthesis and that certain steps, in actual practice, are capable of being merged, conducted simultaneously, or effected in a reverse sequence without materially altering the progress of synthesis.

The following examples recite a precise scheme of total synthesis. It is to be understood that the purpose of this recitation is to further illustrate the total synthesis and not to impose any limitation. 487 29 EXAMPLE 1 Preparation of 4(S)-4-Iodomethylazetidin-2-one STEP A 0ch2o2c nh2'TSOH i_^c°2ch20 $> 0CH2O2C nh2 ICO2CH20 /CH2O2C NHSiMe3 O2CH20 Benzyl (S)-azetidin-2-one-4-carboxylate To a 1000 ml separatery funnel are added di benzyl (S)-aspartate p-toluenesulfonic acid salt (48.6 g, 0.1 mole), ice-cold diethyl ether (300 ml), ice-cold water (100 ml), and ice-cold saturated aqueous potassium carbonate (50 ml). The mixture is shaken vigorously and the layers are separated. The aqueous portion is extracted with more cold diethyl ether (2 x 100 ml). The combined ether solution is washed with brine, dried with magnesium sulfate, and evaporated under vacuum to provide dibenzyl (S)aspartate (31.4 g, 0.1 mole) as a colorless liquid The dibenzyl (S)-aspartate in anhydrous diethyl ether (200 ml) is cooled in an ice-bath under a nitrogen atmosphere. Trimethylchlorosilane (12.7 ml, 0.1 stole) is added to the stirred solution to give a white precipitate. Triethylamine (14.0 ml, .0.1 mole) is then added to the mixture. The cooling bath is removed and the mixture is stirred at room temperature (22-25eC) for 2 hrs. The'mixture is then filtered directly into a 3-neck, χ.ο liter, round bottom flask fitted with a sintered glass funnel, magnetic stirrer, and a vacuum-nitrogen inlet. This operation is carried out under a blanket of nitrogen, care being taken to exclude atmospheric moisture.

The sintered glass' funnel is replaced by a stopper and the ether is evaporated under vacuum with stirring to provide dibenzyl (S)-N-trimethylsilylaspartate (35.5 g 0.092 mole) as a slightly hazy oil.

Anhydrous diethyl ether (250 ml) is added to the flask containing the silyl derivative and the magnetic stirrer is replaced by a mechanical stirrer. The resulting solution ie stirred under a nitrogen atmosphere with ice-bath cooling.

Ethereal ethyl magnesium bromide (34 ml of a 2.94 M solution, 0.1 mole, is added dropwise over 40 min. to give a cream colored, Visage precipitate.

The cooling bath is removed and the mixture is stirred at room temperature. After 1.5 hrs, a viscous gum forms. The mixture is allowed to stand overnight at room temperature.

The mixture is then cooled in an ice-methanol bath while ammonium chloride saturated 2n hydrochloric acid (100 ml) is added slowly with stirring. The resulting mixture is diluted with ethyl acetate (100 ml) and water (100 ml) and the layers are separated. The aqueous portion is extracted with more ethyl acetate (3 χ 100 ml). The combined organic solution is washed with water (200 ml), % aqueous sodium bicarbonate solution (100 ml) , water (100 ml), and brine, dried with magnesium sulfate, and filtered. Evaporation of the solvent under vacuum gives an orange oil interspersed with a fine, granular precipitate (25.3 g) . This material is dissolved in warm chloroform (75 ml), diluted with petroleum ether (125 ml), seeded, scratched, and cooled in an ice-bath. The precipitate is collected, washed with petroleum ether, and dried under vacuum to give benzyl (S)-azetidin2-one-4-carboxylate (3.85 g) as an off-white solid mp 136-139°C. The mother liquors and washings are combined, diluted with petroleum ether to 500 ml, seeded, and left in a refrigerator for several days. The resulting precipitate is collected, washed with petroleum ether, and dried under vacuum to give additional product (0.82 g) as pale yellow crystals. Recrystallization of a sample from chloroform-petroleum ether gave the product as small, white flakes: mp 141-143°; (a]D= -43.4° (C3.275 in CHC13); IRiCHClj) 3425, 1778, 1746 cm1? 1H NMR(CDCl3) «f3.00 (ddd, 1,J- 1.9, 3.2, and 14.6 Hz, H-3a) ,^3.35 (ddd, 1, J - 1.5, 5.4, and 14.6 Hz, H-3b),^4.20 (dd, 1, J - 3.2 and 5.4 Hz, H-4) , ¢^5.22 (s, 2, OCH^Ph, , <£*6.48 (m, 1, NH) , 7.38 (s, , phenyl); mass spectrum m/e 205 (M+), 163, 91, 70, 43.

Anal. Calcd. for C, 64.38; H, .40? N, 6.83. Found: C, 64.10? H, 5.70? N, 6.77.

STEP Β COjCH^ cf' OK (S)-4-Hydroxymethylazetidin-2-one’i Sodium borohydride (3.69 g, 97.5 mmol) is added in one portion to a suspension of benzyl 4(S)azetidin-2-one-4-carboxylate (20,0 g, 97.5 mmol) in 300 ml of absolute methanol at O’C. The mixture is then allowed to warm slowly with periodic cooling being supplied to keep the internal temper10 ature < 30eC. After stirring for 2 hr., glacial acetic acid (23.4 g, 390 mmol) is added and the reaction mixture is concentrated under vacuum. The residue is treated with 500 ml of chloroform and filtered. The filtrate is concentrated under vacuum and the residue is chromatographed on 250 g of silica gel (4:1, chloroform: methanol) to yield 9.62 g (98%) of 4(S)-hydroxymethylazetidin-2-one as a white solid: m.p. 51-53*C; [a]D*+68.0° (C=2.676 in CHCl^ IR (CHClj) 3410, 1765 cm1 1H NMR (CDC13) «a 7.07 (1H, br. S, NH),/4.05 (1H, br. s, OH), J 3.77 (2H, m H4, H-5a or b) , cf 3.58 (1H, dd, J-ll, 6, H-5a or b), 2.97 (1H, ddd, J= 14.5, 4.8, 1.3, H3b),cf2.7 (1H, br. d, J-14.5, H3a); mass spectrum m/e 101 (M+), 83. Ο OSCH, η ο 4(S)-4-Methanesulfonyloxymethyl azetidin-2-one Methane,sulfonyl chloride (11.46 g, 100 < mmol) is added dropwise by syringe to a solution of 4(S)-4-hydroxymethyl a2etidin-2-one (10.1 g, 100 mmol) and triethyl amine (10.1 g, 100 mmol) in 15 ml of dry methylene chloride at 0°C. (Warming is necessary in order to initially solubilize the alcohol. The resulting solution is then cooled to 0°c prior to addition of the other reagents).

The resulting solution is stirred at 0ec for 1 hr. during which time a voluminous precipitate is produced. At the end of this time, the reaction mix15 ture is filtered and the filtrate is concentrated under vacuum. The two solid residues are combined and treated with 500 ml of chloroform. The resulting mixture is filtered to yield substantially pure 4(S)-4-methanesulfonyloxymethyl azetidin-2-one as a 2o white solid. The filtrate, which contains most of the triethylamine hydrochloride, is concentrated under vacuum and chromatographed on 200 g of silica gel (4si chloroformsmethanol) to yield an additional quantity of mesylate. This material is combined with that obtained previously and recrystallized 4872S from chloroform to yield 15.57 g (87%) of 4(S)-4methanesulfonyloxymethylazetidin-2-one as colorless needles: m.p. 109.5-110.5*C; (α)0«=+25.8β (0=1.025 in H2O)j NMR (D2O) ¢/ 4.62 (1H, dd, J-11.2, 3.0, H-5a or b),¢/4.43 (1H, dd, J= 11.2, 6, H-5a ox b) , sf 4.12 (1H, m , H<) e/3.26 (3H, s -SCHO , ¢/3.19 tt (1H, dd, J*=15, 4.5» H3b) . ¢-/2.88 (1H, dd, J=15, 2.5, H3a) ; mass spectrum 10 m/e 179 (M+),136; Anal: Calc: C, 33.51: H, 5.06; N, 7.82; S, 17.89 Pound: C, 33.54,· H, 5.08; N, 7.72; S, 17.93 STEP D acetone Nai 4(S)-4-Xodomethylazetidin-2-one A mixture of 4(S)-4-raethanesulfonyloxy azetidin 2-one (11.8 g, 65.9 mmol) and powdered sodium iodide (19.8 g, 132 mmol) in 130 ml of acetone is heated at reflux for 6 hr. The resulting reaction mixture is concentrated in vacuo, treated with 200 ml of chloroform and filtered.

The filtrate is washed with 2 X 50 ml of water and dried over magnesium sulfate. The organic phase is filtered, concentrated in vacuo, and chromatographed on 250 g of silica gel (ethyl acetate) to 5 yield 11.94 g (86%) of 4(S)-4-iodomethyl-azetidin2-one as a white solid. This material is recrystal lized from ether-petroleum ether to yield white crystals: mp 91-92°C; [a]^“ -23.7° (C= 1.354 in CHC13); IR (CHC13) 3450, 1765 cm*1»lH NMR (CHC13) 6.13 (hrs, N-H), / 3.94 (m, 1 H, He) ,3.36 (m, 2H, Hd and e), ef3.16 (ddd, 1H, J«14.9, 5.4, 2.3, Ha), cf2.72 (d, d, d, 1H, J*14.9, 2.1, 2, Hb) mass spectrum m/e 211 (M+), 168,' 142, 127, 84. 8 729 EXAMPLE 2 Preparation of (4S)-1-(t-Butyldimethylsilyl)-4-iodomethylazetidin-2-one t-butyldimethylchlorosilane (7.51 g, 49.8 mmol) is added in one portion to an ice-cold, stirring solution of (4S)4-iodomethyl-azetidin-2-one (10.Og, 47.4mmol) and triethyiamine (5.04g, 49.8 nmol) in anhydrous dimethylformamide (100 ml). A voluminous white precipitate forms almost immediately. The reaction mixture is stirred at 0-5® for 1 hour and then allowed to warm to room temperature. Most of the solvent is removed under vacuum to give a residue which is partitioned between diethyl ether (250 ml) and water. The ethereal phase is washed with 2.5N hydrochloric acid (50 ml), water (3x50 ml), and brine, dried with magnesium sulfate, filtered, and evaporated under vacuum to provide (4S)-1-(t-butyldimethylsilyl)-4-iodomethyl-azetidin-2-one (15.1 g) as a white solid. Recrystallization from petroleum ether - ethyl ether gives the product as colorless plates, mp 71-72®; n.m.r. (CDCl-j), £f3.8 (m,l) , £ 2.6-3.6 (2 overlapping d of AB, 4) Jl. 0 (S,9) O.3(S,6), 487 29 EXAMPLE 3 Preparation of (4S)-l-(t-Butyldimethylsilyl)-4(2,2,2-tri(methylthio)-ethyl)azetidin-2-one SCH n-Butyllithium (19.4 ml of 2.5M hexane/solution, 48.5 mmol) is added slowly by syringe to a solution of tri(methylthio,methane (7.47g, 48.5 mmol) in 150 ml of freshly distilled THF at -78eC. The resulting solution is stirred at -78*C for 30 min. prior to the addition of a solution of (4S)-1(tert-butyldimethylsilyl)-4-iodomethylazetidin-2-one (15.Og, 46.15 mmol) in 50 ml of THF. This solution is stirred at -78°C for 30 min., then quenched by addition of saturated aqueous ammonium chloride solution. The reaction mixture is allowed to warm to room temperature, then poured into ether (250 ml), washed with water (2x100 ml) brine (100 ml) and dried over magnesium sulfate. Removal of solvents in vacuo gives an oil which is crystallized from petroleum ether to give 13.3g (82%) of (4S)-1-(t-butyldimethylsilyl) -4-(2,2,2-tri(methylthio)ethyl)azetidin-2-one as colorless prisms, m.p. 61-62°C. IR(CHC13,CM ^) 2918, 2850,1730; n.m.r. (CDC13)S' 4.0(m,l),5 3.35(dd,1,J=5,5,16), <Γ2.83(dd,1,J=3,16)f 2.5(ABq,2)ί 2.15(5,9), J 0.93(s,9), io.25(s,6).

EXAMPLE 4 Preparation of (3S, 4R)-1-(t-butyldimethylsilyL)-3l(R )-1-hydroxyethylJ-4-[2,2,2-tri(methylthio)ethyl)az etidin-2-one SCH3 SCH3 HO sch3 ^sch3 0' n-Butyllithium (14.8 ml of 2.5N hexane'solution, 37.0 mmol) is added by syringe to a solution of diisopropylamine (3.74g, 37.0 mmol) in 180 ml of freshly distilled tetrahydrofuran at -78*C. The resulting solution is stirred at -78 *C for 15 min prior to the addition of a solution of (4S)-l-(tbutyldimethylsilyl)-4-(2,2,2-tri(methylthio)ethyl] azetidin-2-one (12.34g, 35.16 mmol) in 35 ml of tetrahydrofuran. This solution is stirred at -78eC for 10 min prior to the addition of acetaldehyde (4.62g, 105 mmol). The solution is stirred for an additional 5 min. at -78*C and then quenched by addition of saturated aqueous ammonium chloride solution, and allowed to warm to room temperature. The mixture is poured into 250 ml of ether and washed with water (2 x 100 ml) and brine and dried over magnesium sulfate.

Removal of solutions in vacuo gives an oil which is chromatographed on a silica gel column (1:1 ethers petroleumether) to give (3S,4R)-l-(t-butyldimethylsilyl)3- [ (R) -1-hydroxyethyl] -4- (2,2,2ytri (methylthio) ethyl] azetidin-2-qne L7.,Qg, 50,4%). at Rj«Q,2. Theproduct can he recrystallized from petroleum ether. Alternatively, the trans R product can be isolated from the crude reaction mixture by direct crystallization from a petroleum ether solution. 8 7 2 9 EXAMPLE 5 Preparation of (3S,4p.)-1-(t-Butyldimethylsilyl)-3-(1oxoethyl)-4-(2,2,2,-tri(methylthio)ethyl]azetidin-2-one A. n-Butyllithiuai (2.43 ml of 2.4m solution, 5.84 mmol) is added by syringe to a solution of diisopropylamine (591 mg, 5.84 mmol) in 25 ml of freshly distilled tetrahydrofuran at -78°C. The resulting solution is stirred at -78°C for 15 minutes prior to the addition of a solution of (4R)-1-(t-butyldimethylsilyl)-4-[2,2,2-tri(methylthio) ethyl]azetidin-2-one (1.00 g, 2,85 mmol) in tetrahydrofuran (5 ml). This solution is stirred at -78°C for 15 minutes, then added via a Teflon tube to a mixture of N-acetylimidazole (642 mg, 5.84 mmol) in 25 ml of THF at -78°C.

The resulting yellow reaction mixture is stirred at -78°C for 10 minutes, then quenched by addition of saturated aqueous ammonium chloride solution. The mixture is diluted with ether (200 ml) and washed with 2.5N hydrochloric acid solution (50ml), water (50 ml) and brine (50 ml) and dried over magnesium sulfate. Removal of solvents in vacuo gives a yellow oil which is chromatographed on silica gel (30% ether in petroleum ether) to yield (3S, 4R)-1-(t-butyldimethylsilyl) -3-(1-oxoethyl)-4-12,2,2-tri(methylthio)ethyl) azetidin-2-one. n.m.r. (CDCl^) S' 4.42(m,l),f 4.32(3,1)/ 2.35 (m,2) ,/ 2.32( s,3) , /2.2(5,9), f 0.98(3,9) ,f 0.3 (2s,6).

B. Trifluoroacetic anhydride (400 mg., 1.905 mmol) is added by syringe to a solution of dimethyl sulfoxide (2.53 mmol) in dry methylene chloride (5 ml) at -78°C. The resulting mixture is stirred at -78°C for 30 minutes prior to the addition of a solution of ( 3rs, 4R)-l-(tbutyldimethylsilyl)-3- [( rs )-1-hydroxyethyl]-4-[2,2,2triimethylthio)ethyl] azetidin-2-one (500 mg., 1.27 mmol) in dry CH2C12(1 ml). The resulting solution is stirred for 30 minutes prior to the addition of triethylamine (360 mg., 3.56 mmol). The cooling bath is removed.

After 40 minutes, the reaction mixture is diluted with CHjClp, washed with water and brine and dried over magnesium sulfate. Removal of solvents in vacuo gives an oil which is purified as above. Yields 432 mg. (86%). 8 7 2 9 2C EXAMPLE 6 Preparation of (3S,4 R)-1-(t-butyldimethylsilyl)-3-[(R)1-hydroxyethyl]-4-(2,2,2-tri(methylthio)ethylJ azetidin-2one -z SCH3 I lsc SCH3 » (Irtjda no·-1'/ K-Selectride>^ (3.64 ml of 0.5M, 1.82 mmol) is added by syringe to a mixture of potassium iodide (126 mg.,0.758 mmol) and (3S,4R)-l-(t-butyldimethylsilyl)-3-(l-oxoethyl)-4(2,2,2-tri(methylthio)ethyl]azetidin-2-one (298 mg, 0758 mmol) in freshly distilled ethyl ether (8 ml) at room temperature. The resulting mixture is stirred at room temperature for 2.5 hours, then guenched by the addition of acetic acid (218 mg., 3.64 mmol). The resulting mixture is diluted with ethyl acetate (25 ml) and filtered through celite. Removal of solvents in vacuo gives an oil which is chromatographed on silica gel (ether:petroleum ether) to yield 252 mg of (3S, 4R)-1-(t-butyldimethylsilyl)-3-[(R)-1-hydroxyethyl]4-[2,2,2-tri(methylthio) ethyl] azetidin-2-one. N.M.R.

(R isomer, CDC13+D2O)£ 4.15(dq,l),^3.95(ddd,l,J=9.5,2.3), S 3.26 (dd, 1,J=8,2.3) , 6,6),f1.0(s,9),tfo.26(s,6).

EXAMPLE 7 Preparation of (3S,4R)-l-(t-Butyldimethylsilyl)-3^g)-1-hydroxyethyl)-4-carbo«ethoxymethylazetidin-2- Meeuric chloride (12.37g, 45.6 mmol) is added in one portion to a solution of (3S,4R)-1-(t-butyldimethylsilyl) -3- [(R)-1-hydroxyethyl]-4-(2,2,2-tri(methylthio)ethyl] azetidin-2-one (6.0g, 15.2 mmol) in 250 ml of absolute methanol at O’C. The resulting mixture (heavy white precipitate) is stirred at O’C for 3 min., then quenched by addition of sodium bicarbonate (8.99 g,107mmol). This mixture is then filtered and the solid residue is washed with additional methanol. The combined filtrate and washings are concentrated in vacuo and the residue is partitioned between ethyl acetate and saturated aqueous ammonium chloride solution. The organic phase is separated, washed with saturated aqueous ammonium chloride solution, water and brine and dried over magnesium sulfate. 2o Removal of solvents in vacuo gives an oil which is chromatographed on a silica gel column (3:2 cyclohexane: ethyl acetate), to yield (3S, 4R)-1-(t-butyldimethylsilyl)3— [(R)-1-hydroxyethyl)]-4-carbomethoxymethyl azetidin-2one.

EXAMPLE 8 Preparation of (3S,4R)-l-(t-Butyldimethylsilyl)-3l(R)-1-(t-butyldimethylsilyloxy)ethyl]-4-carbomethoxymethylazetidin-2-one t-Butyldimethylchlorosilane (940 mg, 6.25 mmol) is added in one portion to a solution of (3S, 4R)-1(t-butyldimethylsilyl)-3-[(R)-1-hydroxyethyl]-4carbomethoxymethylazetidin-2-one (1.88g, 6.25 mmol) and triethylamine (1.27 g, 6.25 mmol) in 15 ml of anhydrous diemthylformamide at O’C. After 15 min. at O’C the cooling bath is removed and the reaction mixture is stirred at room temperature for 24 hrs.

Ether (100 ml) is added and the mixture is filtered, then washed with 2.5N hydrochloric acid (20 ml), water (3x20 ml) and brine. The organic phase is dried over magnesium sulfate, then concentrated in vacuo.

The residue is chromatographed on silica gel (7:3 petroleum ether:ether) to yield (3S, 4R)-1-(t-butyldimethyl20 silyl)-3- [ (R) -1-(t-butyldimethylsilyloxy)ethyl]-4carbomethoxymethylazetidin-2-one. n.m.r. (CDCl3)/4.1(m,2), if 3.68 (S, 3) , /3.03 (dd,l,J=4 .3,2 .7) ,/ 2.8(ABq,2) ,/1.17 (d,3, J=6.6) /o. 98 (s, 9) /O.89(s,9) ,/θ.23 (s,6) ,/θ. 1 (s, 6) .

EXAMPLE 9 Preparation of (35,4R)-l-(t-Butyldimethylsilyl)-3((R)-1-(t-butyldimethylsilyloxy)ethyl]-4(2-oxoethyl)azetidin-2-one -}- SiO C/%5ir '\ 1 O,CH, -j-sio „ - 'c: '1 Diisobutylaluminum hydride (3.72 ml of 0.91 M in hexane, 3.38 mmol) is added slowly by syringe to a solution of (3S, 4R)-1-(t-butyldimethylsilyl)-3 - ((R)-1-(tbutyldimethylsilyloxy)ethyl]-4-carbomethoxymethyl azetidin-2-one (936 mg, 2.26 mmol) in 25 ml of freshly distilled toluene at -78’C. The resulting solution is stirred at -78’C for 3 hrs., then quenched by addition of 2.5n hydrochloric acid (5 ml). The resulting mixture is stirred for 2 min., then poured into a separatory funnel containing 100 ml of ether and 50 ml of 1.25N hydrochloric acid saturated with tartaric acid. The organic phase is separated and the aqueous phase is extracted with ether (2x50 ml,. The combined organic phases are washed with brine and dried over magnesium 2q sulfate. Removal of solvents in vacuo gives a white solid which is reerystallized from ether-petroleum ether to give (3S,4R)-1-(t-butyldimethylsilyl,-3-((R)-1(t-butyldimethylsilyloxy)ethyl]-4-(2-oxoethyl)azetidin-2-one. m.p. 115-116’C; n.m.r.(CDCIJ / 4.1 (m,l), ^4.03(ra,1),/ 2.7-3.2(m,3) ,/ 1.23(0,3,1=6.4),/1.08(3,9), /o.9(S,9) ,/o.25(s,6) ,/o.l(s,6),/9.83 (t, 1, J=1.4). 487 29 EXAMPLE 10 Preparation of (3S, 4R)-1-(.t-Butyldimethylsilyl)-3((R)-1-(t-butyldimethylsilyjoxy,ethyl]-4-(3benzyloxycarbonyl-2T4iydroxypropyl)azetidin-2-one n-Butyllithium (1.81 mmol) is added by syringe to a i solution of diisopropylamine (1.81 mmol) in 9 ml of freshly distilled tetrahydrofuran at -78°C. The resulting solution is stirred for 15 min at -78°C.

Benzyl acetate (1.81 mmol) is then added dropwise by syringe and the resulting solution is stirred'at -78°C for 20 min. A solution of (3S, 4R)-1-(t-butyldimethylsilyl) -3-((R)-1-(t-butyldimethylsilyloxy)ethyl]-4(2-oxoethyl)azetidin-2-one (1.64 mmol) in 3 ml of anhydrous tetrahydrofuran is added slowly by syringe.

The reaction mixture is stirred at -78°C an additional 15 min and then quenched by addition of saturated aqueous ammonium chloride solution. Ethyl acetate (50 ml) is added and the organic phase is separated, washed with water (2x10 ml) and brine and dried over magnesium sulfate. Removal of solvents in vacuo gives a white solid which is chromatographed on a short silica gel column (40% ether in petroleum ether, to yield (3S,4R)-1-(t-butyldimethylsilyl)-3-[(R)-1-(t-butyldimethyl25 silyloxy)ethyl]-4-(3-benzyloxycarbonyl-2-hydroxypropyl)azetidin-2-one. n.m.r, (CDCl^Jzf 7.32(s,5), J*5,l(s,2), EXAMPLE 11 Preparation of (3S,4R)-l-(t-Butyldimethylsilyl)-3l (R) -1-(t-butyldimethylsilyloxy)ethylJ -4-(3-(4nitrobenzyl)oxycarbonyl-2-hydroxypropyllazetidin-2-one A mixture of (35,4R)-1-(t-butyldimethylsilyl)-3[(R)-1-(t-butyldimethylsilyloxy)ethyl)-4-(3benzyloxycarbonyl-2-hydroxypropyl)azetidin-2-one (1.00 mmol), sodium bicarbonate (1.00 mmol) and 10% Pd/C in 20 ml of 4:1 tetrahydrofuran-HjO is hydrogenated at 40 psi on a Parr shaker for 30 min. The mixture is then filtered through Celite and the catalyst is washed with 10 ml of water. The combined washings and filtrate are concentrated i.v. to 2 ml and lyophilized. The resulting fluffy white solid is dissolved in 5 ml of anhydrous dimethylformamide and p-nitrobenzyl bromide (216 mg, 1.00 rnml) is added in one portion. The resulting solution is stirred at room temperature for 3 hrs, then diluted with ether (50 ml) and washed with water (3x10 ml) and brine 2C and dried over magnesium sulfate. The solvents are removed in vacuo and the residue is chromatographed on silica gel to yield (3S,4R)-1-(t-butyldimethylsilyl) 3(( R) -1-(t-butyldimethylsilyloxy)ethyl)-4-(3-(4-nitrobenzyl)oxycarbonyl-2-hydroxypropyl)azetidin-2-one. n.m.r.(CDCl3) i7.85(2d-arcmatic,4)/5.26(s,2) , EXAMPLE 12 Preparation of (3S,4R)-l-(t-Butyldimethylsilyl)-3[ (R) -1- (t-butyldimethylsilyloxy) ethyl] -4- [3- (4nitrobenzyl)oxyearbonyl-2-oxopropyl]azetidin-2-one Anhydrous chromium trioxide (10.0 mmol) is added to a soution of anhydrous pyridine (20.0 mmol) in 30 ml of anhydrous methylene chloride. After stirring at room temperature for 15 min., the reaction mixture is treated all at once with a solution of (3S,4R)-1(t-butyldimethylsilyl)-3-[(R)-1-(t-butyldimethylsilyloxy) ethyl -4-[3-(4-nitrobenzyl) oxycarbonyl-2-hydroxypropyl]azetidin-2-one (1.00 mmol) in annydrous methylene chloride (8 ml). The resulting mixture is stirred at room temperature for 5 min. The CHjClj layer is decanted from the dark, tarry residue which is triturated with more Cf^Clj· The combined CHjClj phase is concentrated in vacuo. The residue is triturated with ether (100 ml) and the ether extracts are filtered.

The filtrate is washed with 5% aqueous sodium bicarbonate solution, 2.5N HCl, 5% NaHCO^ and brine, dried over magnesium sulfate and concentrated in vacuo to yield (3 S,4R)-1-(t-butyldimethylsilyl,-3-[(R)-1-(t-butyldimethylsilyloxy) ethyl]-4- [3-(4-nitrobenzyl)oxycarbonyl-225 oxopropyl]azetidin-2-one. n.m.x.(CDCl^ltf 7.85(2d-aromatic, 4) , /5.27 (s, 2) ,/4.05 (m,2) , EXAMPLE 13 Preparation of (3S,4R)-3-[(R) 1-hydroxyethyl]-4-[3(4-nitrobenzyl)oxycarbonyl-2-oxopropyl]azetidin-2-one I (3S,4R)-1-(t-butyldimethylsilyl)3-[(R)-1-(t-butyldimethylsilyloxy) ethyl]-4-(3-(4-nitrobenzyl)oxycarbonyl2-oxopropyl]azetidin-2-one (7.9 mmol) is dissolved in 160 ml of 9:1 (v/v) methanol-water and cooled to O’C. Concentrated hydrochloric azid (2.75 ml) is added and the resulting solution is stirred at O’C for 15 min., then allowed to warm to room temperature. The solution is stirred at room temperature for 2.5 hrs, then diluted with ethyl acetate (200 ml) and washed with water, saturated aqueous sodium bicarbonate solution and brine, dried over magnesium sulfate and concentrated in vacuo to yield (3S, 4r)-3-[(R)1-hydroxyethyl]-4-[3-(4nitrobenzyl)oxycarbonyl-2-oxopropyl]-azetidin-2-one. 487 29 EXAMPLE 14 Preparation of (3S,4R)-3-((R)-1-Hydroxyethyl)-4- [3-(4-nitrobenzyl) oxycarbonyl-2-oxo-3-diazopropyl]azetidm-2-one -3 iriethylamine (263 mg, 2.6mmol) is added by syringe to a mixture of (3S,4R)-3-( (R) - 1-hydroxyethyl]-4-(3-(4-nitrobenzyl) oxycarbonyl-2-oxopropyl] azetidin-2-one.,(253mg, 0.72 mmol) and p-carboxybenzene sulfonylazide (196 mg, 0.84 mmol) in dry acetonitrile (6 ml) atO°C. When addition is complete the cooling bath is removed and the reaction mixture is stirred at room temperature fpr 1 hour. The mixture is then diluted with ethyl acetate (50 ml) and filtered. The filtrate is concentrated in vacuo and the residue is chromatographed on a short silica gel column (ethyl acetate) to yield 222 mg, (81% overall from (3S_, 4R) -1-(t- butyldimethylsilyl)-3-[(R)-1-(t-butyl dimethylsilyloxy)ethyl]-4- [3(4-nitrobenzyl)oxycarbonyl-2-oxopropyl)azetidin-2-one) of (3S, 4R) -3-(R)-(1-hydroxyethyl)-4-£3-(4-nitrobenzyl)oxycarbonyl2-oxo-3-diazopropyl]azetidin-2-one as a white solid m.p. (dec.) 163°C. IR(CHC13, CM-1) 3410, 2132, 1756, 1718, 1650, 1350, 1280, 1120; n.m.r. (CDCl-j)/7.9 (2d-aromatic, 4) ,/5.4 (s, 2) , /6.2 (hrs,1) J4.1(m,2) ,$2.6-3.6 (ro, 4) ,^1-32 (d,3, J=6.2) .

EXAMPLE 15 Preparation of (5R,6S)p-Nitrobenzyl 6-[(R)1-hydroxyethyl]1-azabicyclo[3,2.0]heptan-3,7-dione-2^carboxvlate_ A suspension of (3S,4R)-3-I(R)-1-hydroxyethyl]-4-[3(4-nitrobenzyl)oxycarbonyl-2-oxo-3-dia^opropyl]azetidin2-one (56.4mg, 0.15 mmol] and rhodium (II) acetate (0.1 mg) in dry benzene (3ml) is deoxygenated by bubbling through nitrogen for 10 minutes. The mixture is then heated to 78°c for 1 hour. During heating the solid starting material gradually goes into solution. The mixture is then cooled, filtered to remove the catalyst, and the filtrate is concentrated in vacuo to yield (5R, 6S) p-nitrobenzyl 6-[(R)1-hydroxyethyl] -1-azabicyclo [3.2.0] heptan-3,7- dione -2-carboxy late, 51 mg. (98%) as a colorless oil which slowly crystallized at room temperature (22’C).

Physical Properties: OH PNB= p-nitrobenzyl n.m.r.: (300MHz, CDCl,)/8.26, 7.54 (aromatic, 4), 5.29 (AB,2), 4.77 (s,l), 4.32(dq,I,J=6.6,7) , 4.16(ddd,1,J=7,7.5,2.2) , 3.21(dd,1,J=7,2.2), 2.94(dd,1,J=19.5,7) 2.50(dd,l,J= 19.5,7.5), 2.2(brs,l), 1.37(d,3,J=6.6). l. R.: (CHC13,CM-1) 1770, 1758, 1610, 1522, 1353 m. p. 110-lll°C 8 7 29 EXAMPLE 16 Preparation of £-Nitrobenzyloxycarbonylaminoethanethiol HSz%zNH2· HC1 Cl-COCH, « ί 0 -0-no2 _\ HSz\/ NHCOjPNB To 600 ml diethyl ether (EtjO) - 75 ml HjO in an ice bath 5 with stirring is added 3.2 g cysteaminp hydrochloride (mw = 114; 28.1 mmole). A solution of 7.14 g NaHCO^ (raw = 84; 85 mmole) in 75 ml H2O is added. The ice bath is removed, and at room temperature a solution of 6.75 g onitrobenzylchloroformate (mw = 216; 31.3 mmole) in 270 ml Et2O is added dropwise over a period of one hour. After additional minutes, the layers are separated. The ether layer is extracted with 150 ml 0.25 N HC1, and then with 200 ml brine. Each aqueous layer is then backwashed successively with 100 ml EtjO. The combined Et2O layers are dried over anhydrous MgSO^, filtered, and concentrated under a N2 stream. The crystalline residue is slurried in a small amount of ether, filtered, and the pale yellow crystals are dried under high vacuum to give 4.7 g. £nitroben2yloxycarbonylaminoethanethiol (65% yield). 2o NMR (CDClj); 8.18 (d, J=8Hz, aromatic protons ortho to nitro), 7.47 (d, J=8Hz, aromatic protons meta to nitro), .27 (-NH-), 5.20 (s, _CH2-NH-), 2.67 (m, -CH2-SH), 1.35 (t, J=8.5Hz, -SH) in ppm downfield from TMS. IR (CHC1, ~ _i 3 solution): carbonyl- 1725 cm . M.S.: molecular ion-256, (M-47) at 209, (M-136) at 120, +CH20pNO2 at 136.

EXAMPLE 17 Preparation of (5R,6S) p-Nitrobenzyl 3-[2-(p-nitrobenzyloxycarbonyl) amino ethylthio]-6- C(R)-1-hydroxyethyl] -1-azabicyclo P, 2. 0]-hept-2-en-· 7— one-2-carboxylate Sz-^.NHC02PNB COjPNB (5R,5S) p-Nitrobenzyl 6- £(R)1-hydroxyethyl] -1-azabicyclo 5. 2. 0]heptan-3,7-dione-2-carboxylate (51 mg, 0.147 mmol) is dissolved in acetonitrile (3 ml) and the resulting solution is cooled to O°C. Diisopropylethylamine (22 mg, 0.17 mmol) is added by syringe and the resulting solution is stirred at 0°C for 1 minute prior to the addition of a solution of freshly recrystallized p-toluene sulfonic anhydride (51 mg., 0.156 mmol) in dry acetonitrile (1 ml). The resulting solution containing the isolable intermediate. (5R,6s)}*·” nitrobenzyl-3-(|»-toluene sulfonyloxy-6-((R,-1-hydroxyethyl)-1azabicyclo (3,2.0) hept-2-en-7-one-2-earboxylate is stirred at 0°C for 1 hour , then cooled to -25°C. Diisopropylethylamine (80.5 mg, 0.624 mmol) is added by syringe followed shortly thereafter by a solution of N-p-nitrobenzyloxycarbonylcysteamine (40 mg, 0.156 mmol, in 1 ml of dry acetonitrile. The reaction mixture is then stored in a refrigerator for 70 hr. The mixture is diluted with 25 ml of ethyl acetate washed with brine and dried over magnesium sulfate.

Solvents are removed in vacuo to yield a yellow oil which is chromatographed on a silica gel plate (ethyl acetate, R^=0.4) to yield (5R, 6S) p-nitrobenzyl-3-[2-(pnitrobenzyloxycarbonyl)amino ethylthio]-6-[(R)-1-hydroxyethyl] -1-azabicyclo [3,2.0]-hept-2-en- 7- one-2-carboxylate as a yellow solid, m.p. 167-169°C. IR(Nujol mull) 1773 and 1690 cm-1,· n.m.r. (CDClj) 67.54-8.26 (overlapping ABg,4) , 65.40 (ABq,2), 65.22 (s,2) ,64.27(m,2) , 63.47 (m) , 6 3.23 (dd, 1) , <53.14 (dd, 1) 63. 40(dd,l),63.04(m,2),61.37(d,3).

EXAMPLE 18 Preparation of Thienamycin OH A. rfV <)-N--i-Cl ^NHCOjPNB COjPNB 9^Χζνη2 co2h A mixture of N-p-nitrobenzyloxycarbonyl thienamycin p5 nitrobenzyl ester (lOmg, 0.017 mmol) and 10% Pd/C-Bolhofer type in tetrahydrofuran (2ml), 0.1M dipotassium hydrogen phosphate solution (1.4 ml) and 2-propanol (0.2ml) is hydrogenated at 40 psi on the Parr shaker for 30 minutes. The mixture is then filtered and the catalyst is washed with water (3x3ml) . The combined filtrate and washings are extracted with ethyl acetate-ethyl ether then concentrated to w3ml and lyophilized. The resulting white powder is identical to natural thienamycin in all respects.

Claims (6)

1. CLAIMS :1. The compound: wherein a) R R 8 is R 9 is b) R is R 8 is hydrogen or a protecting group, hydrogen and -ch 2 i, a protecting group, -CH X CH and is -CH 2 C(SR> 3 wherein R is selected from lower alkyl having 1-6 carbon atoms, aryl and aralkyl having 6 to 10 carbon atoms, and wherein R can be the same or different, c) R 1 is hydrogen, R 8 is OH -CH and 0 R 9 is -CH 2 -C-CH 2 -CO 2 R 7 wherein R 7 is a protecting group, d, R 1 is hydrogen, R 8 is OH -CH and 0 q w 7 7 R y is -CH.-C-C—CO.R wherein R is a protecting group. 2. N 2 487 29

2. The compound: wherein is hydrogen or a protecting group.

3. The compound: wherein is a protecting group and R is selected from lower alkyl having 1-6 carbon atoms, aryl and aralkyl having 6 to 10 carbon atoms and R can be the same or different.

4. . The. compound : wherein R 7 is a protecting group.

5. The compound:

6. A process for preparing a compound according to Claims 1 to 5 which comprises a) treatment of a compound I-| Z ^oso 2 ch 3 >- NH 5 with sodium iodide and inserting a protecting group if desired; b) alkylation of a compound with acetaldehyde in a solvent with a strong base; removal c compound 1 2 c, removal of the protecting groups R and R of a OR co 2 r' by acidic hydrolysis in a solvent; d) treatment of a compound OH Jl Λ fi •NH co 2 r 7 in a solvent with an azide in the presence 1 7 wherein R, R and R are defined as in claim of a base