IE42357B1 - Polycyclic antidepressants - Google Patents

Description

This invention relates to tetracyclic antidepressants, dibenzocycloheptenes with a nuclearly fused cyclopropyl moiety.

Dostert and Kyburz (U.S. Patent No. 3,803,234) disclose N-(aminoalkyl or heterocyclic aminoalkyl) dibenzocycloheptene imine derivatives.

Judd (U.S.Patent No. 3,349,128) discloses dibenzocycloheptenes having an aminoalkylene ether attached to the imino nitrogen but without the nuclear fused cyclopropyl moiety.

The following patents relate to compounds with the same basic carbon skeleton but different substituents in the 6-position: Coyne (U.S.

Patent No. 3,574,199), aminoalkyl or aminoalkylidene; Coyne (U.S.Patent Nos. 3,547,933 and 3,658,908), hydroxy or lower alkanoyloxy and various aminoalkyl groups; Remy (U.S.Patent No. 3,475,438), N-alkyl piperidine; Uyeda (Belgian Patent No. 805,433 and French Patent No. 2,201,089), alkylated imine.

Mental illness encompasses both psychoses and neuroses. Symptoms requiring treatment include depression, anxiety, agitatidrt, and hallucinations. Among the drugs used particularly for treatment of both reactive and endogenous depressions are monoamine oxidase (MAO) inhibitors, such as iproniazide, tranylcypromine, nialamide, phenelzine, and par20 gy1ine,and the non-MAO-inhibiting tricyclic aromatic dibenzazepines, such as imipramine, and dibenzocycloheptenes such as amitriptyline.

All of these drugs have adverse side effects that limit their usefulness. MAO inhibitors may benefit milder forms of depression, but the risk of serious toxic effects is a strong argument against their use. They may cause liver damage and acute hypertension, especially if given in conjunction with cheese, bananas, or other amine-containing foods. The MAO inhibitors may also cause tremors, insomnia, hyperhydrosis, agitation, hypermanic behavior, confusion, hallucinations, convulsions and orthostatic hypotension. They frequently cause dizzi30 ness, vertigo, headache, inhibition of ejaculation, difficulty in urin- 2 4 23 5 7 ation, weakness, fatigue, dry mouth, constipation and blurred vision. Imipramine may cause blurred vision, dryness of mouth, constipation, urinary retention, orthostatic hypotension, respiration depression, myocardial infarction, and congestive heart failure. Similar diffi5 culties are experienced with amitriptyline.

There is a continuing need for psychotherapeutic agents that have fewer side effects than the drugs in use today; also for psychotherapeutic agents that have different modes of action than presently used agents, since none of these is completely effective.

The present invention results from efforts to develop new, relatively safe and effective psychotherapeutic compounds with low side effects.

According to this invention there are provided novel compounds of formula I and their pharmaceutically suitable salts, processes for their manufacture, compositions containing them, and methods of using them to alleviate depression in mammals. For legal reasons we claim hereinafter a method of alleviating depression in mammals other than man, but we believe that our compounds are primarily of interest for human treatment. x and . Y = H, F, Cl, Br, Cj—C4 alkyl, Ci—C„ alkoxy, CF3, CH3S, ch3so2 or SO2N(CH3)2, provided that at least one is H; Z = C2—C3 alkylene; Rj and R2 independently = H, Cx—C4 alkyl, or together with the nitrogen 25 atom to which they are attached can form a ring: - 3 42357 in which W is —(CH2)^—, — (CH2)s—, or _(CH2)20(CH2)2— and their pharmaceutically suitable salts.

Compounds preferred because of their high degree of activity are those in which Ri and R2 = H or CH3.

More preferred are those in which X and Y = Η, Z = ethylene, and Ri and R2 = H or CH3.

The compound most preferred is that in which X and Y = Η, Z = ethylene, and Ri and R2 = H.

Pharmaceutically suitable acid addition salts of these compounds include those made with physiologically acceptable acids that are known in the art; such salts include e.g. hydrochloride, sulfate, ni-; trate, phosphate, citrate, tartrate and maleate.

Compounds of this invention may be prepared as shown in the following general reaction scheme. - 4 42357 in which R1( Rg, X, Y and Z are as previously, defined and M is an alkali metal.

Another method of preparing some of the compounds involves reaction of the oxime of a dibenzocyclopropacycloheptene in the form of its alkali metal salt, e.g., sodium, with ethylene oxide to give the corresponding O-e-hydroxyethyloxuue which is converted to its tosylate by reaction with jj-toluene-sulfonylchloride. The tosylate reacts with a hydrogen bearing amine to give the aminoalkyl oximes as shown below: Another method for the preparation of the O-g-hydroxyethyloxime of dibenzocyclopropacycloheptene is the treatment of l,1a,6,10b-tetrahydrodibenzo{a,e}cyclopropa {c} cyclohepten-6-one dimethylketal with carboxymethoxylamine hemihydrochloride to give the O-α-carboxymethyloxime derivative. Reduction of this acid with borane-THF complex or borane dimethylsulfide complex gives the Ο-β-hydroxyethyloxime as shown below: NOCH2CH2OH - 5 42357 The nuclearly substituted 1,1-dichloro -1,la,6,10b-tetrahydrodibenzo(a,e}cyclopropaic}cyclohepten-6-ones used as precursors can be prepared by treating 5H-dibenzo{a,d}-cycloheptene-5-ones under anhydrous conditions with sodium methoxide and ethyltrichloroacetate or heating at reflux a benzene solution of the ketone and phenyl trichloromethyl mercury to form 1,l-dichloro-l,la,6,10h-tetrahydrodibenzo{a,e}cyclo- propa{c}cyclohepten-6-ones of the general-formularL Cl xCl I ~T* 0 and subsequently reducing the dichloroketone to the corresponding carb10 inol by treatment in solution with sodium borohydride to produce the alcohol. The gem chlorines and any halogen on the benzene rings can be replaced with hydrogen by treatment of the dichloroalcohol with lithium and tert.-butanol followed by oxidation with chromium trioxide in sulfuric acid or chromium trioxide in pyridine to form the precursors (II).

The basic starting material, 5H-dibenzo{a,d}-cyclohepten-5-one is commercially available. Synthesis of the benzo-substituted analogs can be carried out by adaptation of the synthesis procedures shown in: A.C. Cope et al., J.Am.Chem.Soc. 73, 1673 (1951) W. Treibs et al., Ber. 84, 671 (1951) S. 0. Winthrop et al., J. Org. Chem. 27, 230 (1962).

Another method of preparing the precursor compounds is addition of dichlorocarbene to the commercially available 5H-dibenzo{a,d}cyclohepten5-one by the method described in U.S. Patent No. 3,547,933 to give 1,125 dichloro-1,la,6,10b-tetrahydrodibenzo{a,e}cyclopropa(c}cyclohepten-6one. Treatment of this dichloroketone with POC13—PC15 followed by sodium methoxide in methanol or triethylamine in methanol gives 1,1- 6 42357 d1ch1oro-1,1a,6,1Ob-tetrahydrodi benzoia,e}-cycloprapa{clcyclohepten6-one dimethylketal. Both chlorine atoms can be replaced with hydrogen by reaction with lithium hydride-triethylborane (lithium triethylborohydride) in hexamethylphosphoramide to give 1,la,6,10b-tetrahydrodi5 benzoia,e}cyc1opropa{c}cyclohepten-6-one-dimethylketal. Treatment of this ketal with acid yields 1,la,6,10b-tetrahydrodibenzo{a,e}cyclopropa{ckyclohepten-6-one. Reaction of this ketone with hydroxylamine gives the 1,1a,6,1Ob-tetrahydrodi benzof a,e}cyclopropaf clcyclohepten-6-one oxime. An advantage of this synthesis is retention of substituents, such as halogen, on one or more of the benzene rings.

Another method of preparing the precursor compounds is by treating the respective 5-H-dibenzofa,d}cyclohepten-5-ones with bromoform and base1 or heating at reflux a benzene solution of the ketone and phenyltribromomethyl mercury to form the respective 1,1-dibromo-l,1a,6,10b15 tetrahydrodibenzo{a,e}qyclopropafc}cyclohepten-6-ones of the general 1. 1,1-Dibromodibenzocyclopropacycloheptenone (A, X=Y*H) was first reported by S. Winstein and R.F. Childs, J. Am. Chem. Soc., 89, 6348 (1967).

Treatment of this dibromoketone with POC^—PC1S followed by triethylamine in methanol gives l,l-dibromo-l,la,6,10b-tetrahydrodibenzofa,e}cyc1opropa{c}cyclohepten-6-ones dimethylketal. Replacement of both of the bromine atoms with hydrogen is effected by reflux with lithium alum25 inum hydride in tetrahydrofuran, or lithium in tert-butanol, or lithium hydride-triethylborane (lithium triethylborohydride) in hexamethylphos- 7 42357 phoramide to give l,la,6,10b-tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6-one dimethylketal. Treatment of this ketai with hydroxylamine hydrochloride in pyridine gives the 1,la,6,10b-tetrahydrodibenzo{a,e}cyclopropa(c}cyclohepten-6-one oxime.

The oxime is readily formed by treatment of the 6-oxo compound with hydroxylamine, generally at 50—125° C. for 10—24 hours, in the presence of a tertiary amine such as pyridine as illustrated by Example IA below.

The above-mentioned method of preparing the precursor 1,la,5,10b10 tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6-one oxime by the reaction Of 1,la,5,10b-tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6one dimethylketal with hydroxylamine hydrochloride in pyridine is illustrated by Example lc and may be depicted as follows: are prepared from the oxime by formation of an alkali metal salt, suitably by reaction with a molar amount of an alkali metal hydride, such as readily available sodium hydride. The alkali metal salt is then reacted with a halide, Hal—ZNRXR2, in an inert, anhydrous solvent, such as hexamethylphosphoramide, generally at 10— 50° C for a few minutes to an hour or more. The inorganic alkali metal halide is removed by washing, and the O-substituted oxime separated and purified.

The O-substituted oximes can also be prepared by treating the ketone II or its dimethylketal derivative with the appropriately O-substituted hydroxylamine hydrochlorides in the presence of pyridine (which conveniently is the reaction medium) as shown: - 8 42337 The compounds of this invention have a basic group, i.e., an amino or substituted amino group, that is 2 or 3 carbons removed from the oxygen ether linkage. This amino group is salt forming and facilitates the preparation of aqueous solutions for pharmaceutical use.

In addition, unlike the corresponding imines, these compounds have superior stability under hydrolytic conditions. For example, a solution of 0.6 g of N-(2-methy1aminoethyl)-1,1a,6,1Ob-tetrahydrodibenzola,ekyclopropaiclcyclohepten-6-imine in 20 ml of 0.5M hydrochloric acid was stirred at 25° C for 20 hrs. The solution was cooled and 10 ml of IOS! sodium hydroxide soltuion was added, and the oil was twice extracted with a solution of 25% methylene chloride in ether. The combined organic extracts were thoroughly washed with saturated brine, dried and solvent removed. The NMR spectrum of the residue (0.5 g) indicated about 40% decomposition of the imine to the ketone occurred.

Following the same procedure 1,la,6,10b-tetrahydrodibenzo{a,e}cyclopropaidcyclohepten-6-one 0-{2-(methylam1no)ethylkx1me showed no detectable decomposition. This stability to hydrolysis is advantageous in preparation, storage, and use of pharmaceutical dosage forms.

In the following illustrative examples all parts are by weight and temperatures are centigrade unless stated to be otherwise.

Example 1.

A. 1,la,6,10b-Tetrahydraiiaenzo{a,e}cyclopropa{c}cyclohepten6-one Oxime.

NOH A solution of 6.6 g (0.03 mole) of l,la,6,10b-tetrahydrodibenzoTa,e}cyclopropa{c}cyclohepten-6-one and 8.4 g (0.12 mole) of hydroxylamine hydrochloride in 120 ml of pyridine was held at reflux for 18.5 hrs. The solvent was evaporated under reduced pressure and the residue was dissolved with a mixture of water and methylene chloride. The layers were separated and the organic layer was in turn washed with 10% hydrochloric acid, and saturated brine, dried and solvent evaporated to give 7.4 g of crude 1,la,6,l0b-tetrahydrodibenzoia,e}cyclopropa{c}cyc1o10 hepten-6-one oxime, mp 201° (ex. MeOH, dried at 110°/0.1 mm). The NMR spectrum in CDC13—DMS0-d6: a singlet at «10.9 (IH), multiplet at 6.9— 7.6 (8H), multiplet at 2.1—2.6 (2H) and multiplet at 1.3—1.8 (2H).

Anal. Calcd. for C15H13N0: C, 81.46; H, 5.57; N. 5.95. Found: C, 81.48; H, 5.68; N, 6.08. Mass spec.: Calcd. for C16H13NO: 235.0996. Found: 235.0997.

B. 1 ,la,6,10b-Tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten6-one 0-{2-(dimethylamino)ethyl}oxime.

Z=CH2CH2 R1=R2=CH3 NOCH2CH2N(CH3)2 A dispersion of 2.5 g of 50% sodium hydride τη mineral oil (0.052 mole of NaH) was thoroughly washed with hexane. To the sodium hydride, a solution of 4.8 g (0.020 mole) of l,la,6,10b-tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6-one oxime in 80 ml of anhydrous hexamethyl- - 10 42357 phosphoramide (HMPA) was added and the mixture was stirred at 25° for 1 hr under nitrogen. To the oxime salt thus formed was added 3.8 g (0.026 mole) of anhydrous 2-dimethylanrinoethylchloride hydrochloride and the mixture was stirred at 25° for 72 hrs. The reaction was quenched by the careful addition of 40 ml of water, and then the mixture was poured into excess water. The oil was extracted twice with ether and the combined organic layers were extracted twice with 50 ml of 10% hydrochloric acid. The acidic solution was thoroughly washed with ether, and then treated with an excess of sodium hydroxide solution. The oil was extracted with ether, washed with saturated brine, dried and solvent removed. The residue was triturated with hexane to give 3.8 g of 1 ,la,6,10b-tetrahydrodibenzo{a,e}cyc1opropa{c}cyclohepten-6-oneo -{2 (dimethylamino)ethyl}oxime, mp 69—70° (from cyclohexane). The NMR spectrum (CDC13): multiplet at δ6.9—7.5 (8H), triplet (J = 6Hz) at 4.3 (2H), singlet at 2.2 superimposed on a multiplet at 2.2—3.9 (10H) and a multiplet at 1.3—1.9 (2H). Anal. Calcd. for C20H22N20: C, 78.40; H, 7.24; N, 9.14. Found: C, 78.50; H, 7.01; N, 8.96.

Mass spec.: Calcd. for C20H22N20:306.1731, Found: 305.1694.

C. 1,la,6,l0b-Tetrahydrodibenzo{a,elcyc1opropa{c}cyclohepten6-one Oxime.

A solution of 1.5 g (5.6 moles) of 1,la,6,1Ob-tetrahydrodibenzo{a,e}cyclopropaic}cyclohepten-6-one dimethylketal and 2.5 g of hydroxylamine hydrochloride in 20 ml of pyridine was held at reflux for 18 hr.

The solvent was removed under reduced pressure and the residue was dissolved with a mixture of water and methylene chloride. The layers were separated and the organic layer was in turn washed with 10% hydrochloric acid, saturated sodium bicarbonate solution and saturated brine, dried and solvent removed to give 1,la,6,10b-tetrahydrodibenzo{a,e}cyc1dpropa{c}cyclohepten-6-one oxime. All of the physical properties were identical to those obtained by Example IA. Note: The oxime forms - 11 η «ή «J u a solvate with methanol.

Example 2. 1,1a,6,1Ob-Tetrahydrodi benzoia,e}cyclopropa{c}cyclohepten6-one 0-{2-(dimethylamino)ethy1loxime hydrochloride.

.HCl NOCH2CH2N(CH3)2 l,la,6,10b-Tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6-one 0-{2-(dimethyTamino)ethyl}oxime prepared as in Example 1 was dissolved in ether and saturated with anhydrous hydrogen chloride. The precipitate was filtered and thoroughly washed with ether. The product was re10 crystallized from acetonitrile, and dried at 100° (0.1 mm), mp 169—171°. Example 3. 1,1a,6,lOb-Tetrahydrodibenzoia,e}cyclopropa{c}cyclohepten6-one 0-{2-(methylamino)ethyl}oxime. noch2ch2nhch3 Z=CH2CH2 VH R2=CH3 A dispersion of 1.5 g of 40% sodium hydride in mineral oil (0.025 mole of NaH) was thoroughly washed with hexane. To the sodium hydride, a solution of 2.4 g (0.010 mole) of 1,la,6,10b-tetrahydrodibenz0{a,e}cyclopropaiclcyclohepten-6-one oxime in 40 ml of dry hexamethylphosphoramide (HMPA) was added. The mixture was stirred at 25° for 1 hr under nitrogen. To the oxime salt was added 1.7 g (0.012 mole) of 2-methylaminoethylchloride hydrochloride and the mixture was stirred at 25° for 16 hrs. The reaction mixture was processed as in Example 1 to give 2.5 g of an oil. Chromatography on 25 g of silica gel with ether as the eluent removed unreacted starting oxime. The crude product was - 12 42357 obtained with methanol—1% triethylamine as eluent. Flash distillation (0.1 μ, 180° bath) of the methanol fractions gave l,la,6,10b-tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6-one 0-{2-(methylamino)ethyl}oxime, MP 100—101° C. NMR spectrum (CDC13): multiplet at fi6.8—7.5 (8H), triplet (J = 6 Hz) at 4.2 (2H), triplet (J = 6 Hz) at 2.8 (2H), singlet at 2.4 superimposed on a multiplet at 2.1—2.5 (5H) and a multiplet at 1.3—1.8 (3H). Mass spec: Calcd. for C19H2ON2O: 292,1575; Found: 292.1545.

In Examples 4—11 reaction of l,la,6,10b-tetrahydrodibenzoia,e} cyclopropatclcyclohepten-6-one oxime as its sodium salt with the recited alkylhalide as described in Example 1 gives the indicated product.

Example 4. 1,1 a,6,1Ob-Tetrahydrodi benzo(a,eIcyclopropaiclcyclohepten6-one 0-{3-(dimethylamino)propylloxime.

Z=C3H6 R^^ NOCH2CHzCHzN(CH3)2 This compound was prepared by the process of Example 1 using 3dimethyl aminopropyl chloride hydrochloride as the halogen amino compound. Crystals obtained from its solution in cyclohexane showed a melting point of 83—83.5°.

NMR spectrum (CDC13): multiplet at 67.0—7.5 (8H), triplet (J = Hz) at 4.2 (2H), singlet at 2.1 superimposed on a multiplet at 2.1— 2.7 (10H) and multiplet at 1.3—2.1 (4H). Anal. Calcd. for C21H24N2O: C, 78.71; H, 7.55; N, 8.74. Found: C, 78.79; H, 7.42; N, 8.73, Mass Spec: Calcd. for C21H25N2O (M + 1): 321.1995. Found: 321.2009.

Example 5. 1,1 a,6,1Ob-Tetrahydrodi benzo{a,e}cyclopropaiclcyclohepten6-one 0-{2-(morpholino)ethylloxime. - 13 42357 This compound was prepared by the process of Example 1 using N(2-chlorethyl)morpholine hydrochloride. Melting point 90—91°, cry stallized from isopropyl alcohol.

NMR (CDCI3): multiplet at 67.0—7.6 (8H), triplet (J = 6 Hz) at 4.3 (2H), muTtiplet at 3.5—3.9 (4H), multiplet at 2,2—2.8 (8H) and multiplet at 1.4—1.8 (2H).

Example 6.

I,la,6,10b-Tetrahydrodibenzo{a,e}cyc1opropa{c}cyclohepten6-one 0-{2-(pyrro1idino)ethyl}oxime.

This compound was prepared.using N-(2-chloroethyl)-pyrrolidine hydrochloride. M.P. (ex. i_-propyl alcohol) 96—96.5°.

NMR (CDC13): multiplet at 67.0—1J (8H), triplet (j = 6 Hz) at 15 4.3 (2H), multiplet at 2.1—3.0 (8H) and multiplet at 1.4—1.9 (6H).

Example 7. 1,1 a,6,1Ob-Tetrahydrodibenzo{a,e}cyc1opropa{c}cyclohepten6-one 0-(2-aminoethyl)oxime.

N0CH2CH2NH2 Z=C2Hq Ri=R2=H - 14 42357 This compound was prepared using 2-chloroethylamine hydrochloride; mp 133—134° (ex. iPrOH).

NMR (CDC13): multiplet at ¢7.0—7.5 (8H), triplet (J = 6Hz) at 4.2 (2H), triplet (J = 6 Hz) at 2.9 (2H), multiplet at 2.2-2.6 (2H), multiplet at 1.4—1.9 (2H) and singlet at 1.2 (2H, exchangeable with D20).

Example 8. 1,1a,6,10b-Tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten6-one 0-(2-piperidinoethyl)oxime.

This compound was prepared using N-(2-chloroethyl)-piperidine hydrochloride; mp 75—76° (ex. i_PrOH).

NMR (CDC13): multiplet at «7.0—7.6 (8H), triplet (J = 6 Hz) at 4.3 (2H), multiplet at 2.2—2.9 (8H) and multiplet at 1.3—1.8 (8H).

Example 9. 1,1 a,6,1Ob-Tetrahydrodi benzo{a,e}cyclopropa{c}cyclohepten6-one 0-{2(diisopropylamino)ethy1}oxime. z=c2h1( Ri«R2=CH(CH3)2 N0CH2CH2N{CH(CH3)2}2 This compound was prepared using 2-diisopropylaminoethyl hydro20 chloride; mp 97—98° (ex iPrOH), NMR (CDC13): multiplet at 67.0—7.6 (8H), triplet (J = 7 Hz) at - 15 4.1 (2H), multiplet at 2.2—3.2 (6H), multiplet at 1.4—2.0 (2H) and doublet (J = 7 Hz) at 1.0 (12H).

Example 10. 1,1 a,6,1 Ob-Tetrahydrodibenzo {a ,e Jcycl opropa fc Jcycl ohepten5 6-one 0-{3-(piperidino)propyl Joxime.

This compound was prepared using N-{3-ch1oropropyl)-piperidine hydrochloride. It was flash distilled at 2μ (200° bath).

NMR (CDC13): multiplet at 56.9—7.5 (8H), triplet (j = 6 Hz) at 10 4.2 (2H), and multiplet at 1.1—2.7 (18H).

Example 11. 4-Chloro-1,1a,6,1Ob-Tetrahydrodi benzoia.e JcyclopropaicJcyclohepten 6-one 0-{2-(dimethylamino)ethylJoxime. z=c2h,, Ri=R2=CH3 X=C1 NOCHzCH2N(CH 3/2 Flash distilled at O.lu (160° bath).

NMR (CDC13): multiplet at fi7.0—7.5 (7Η), triplet (J = 6 Hz) at 4.3 (2H), singlet at 2.2 superimposed on a multiplet at 2.1—2.9 (10H) and multiplet at 1.4—1.8 (2H).

The 4-chlorodibenzocycloheptenone oxime was obtained by reaction 20 of 4-chloro-l.la.e.lOb-tetrahydrodibenzofa.eJ-cyclopropatcJcyclohepten- 16 42357 6-one with hydroxylamine hydrochloride as described in Example IA. The sodium salt was then reacted with 2-dimethylaminoethylchloride hydrochloride as in Example IB.

By the method described other nuclearly substituted 1,1a,6,10b5 tetrahydrodibenzo{a,e}cyclopropa{c}cyclohepten-6-one oximes (Column 1 of Table I) react with 2-dimethylaminoethylchloride hydrochloride to yield the products in Column 2 of Table I. - 17 XJ φ Ρ I 1 ο 1 rd ο. ο. φ Ε ο I Η ϋ •-r-» . rO P o. Φ e o Ο *υ & Ο ϋ & 1 ο Ρ“ Ο ο ο 'ο CL Γ*\ r? U Ε >» Ο © φ φ Ο ο ο >> CL ο Ε S >, σ Ο r*~1 ο. © ro •Γ» ΙΟ U Ο ρη © ο pH X CL ‘-Γ-’ pH ο α Η-» © Ο ο Ο to υ «τ» >> ro *© Η-» Μ ρ^η Ε CL Η-· <0 © Φ Ο. >> (0 Ε r~ Ω. Ο Φ Ο. pH ε ο © CU Φ >> Ο L* Q. Ο φ •Γ· Ρ ΡΗ o Χ3 χ: r— Ο. Ο S- X CL φ φ E •V Ρ Ο © Ε ο. <ο Ο ο s Ο- Ό Φ ?? r— Ο- ο η-» pH rd Ο Ο ο Ο Ο ο ο η-» X Ε ο r*~i φ ο φ Ν 5η >» Ο o © •α Ε Φ © ο Β >> ε Ε ΧΖ ο Μ /-H >> >» «Γ» -Γ £ V1-» Φ ©* Φ Ρ I Ε © χζ ε rd κ φ ε C? χ «Η X X) φ Φ 5h rH rO <0 «-ν’ ’ί- ο φ ο Φ Χ2 E Φ Ε I ο χ (Ο χ φ /“»Λ -σ θ' •τ— P Ρ Ν Ο Uv· ο <0 ο Ε <0 Χ5 Φ (O Φ Ε ο ίθ Τζ kr* *>i Ε Η_» Ο Ur/ Ρ χζ φ Ρ- ΙΜ Η-» _Ε ο JZ -σ ε Ο Φ Ε o Ο Φ I Ρ Xi >> C ο Ρ Ν Ρ >> <0 ν ε XJ ε n ε Xi φ •ρ» ΧΖ φ χζ Ν Φ Ε φ χζ Ε ·»“ >> «r— c ·»- σ ε το ρ xa Ρ Ε φ 1 (ΰ Si Φ X ΧΖ ε Φ X ι— ·Ρ“ Ο Φ •f» φ Φ ο _Ω ΡΗ Ε Ε Χ3 Ο Φ to X3 O « Ό S— *σ η Ε ·Γ“ ο <Ρ Ρ •ρ- ρΗ V p— •1— p·-» to “Ο ο ο ο •r— -σ Ε φ φ σ γ— Ρ 5b σ r- « 1 >> c Ε Ε Χ3 ε ο •ρ— Ρ ε Ο >5 Φ XZ O >» ro CM *5 *= Ό •r~ Ο Π3 ε ε I «γ* Ε ΧΖ Ρ P S- XZ Ρ~ '-ν’ <0 ε ε Ε σ ro xa X -α ρ | Φ -σ p Ρ 1 Ε tO χ: to -σ >> >> ρ- ο ««—- >> Φ χζ ε >» ω r— Ο ·£* *Σ“ (0 >> ΧΖ χζ >> Γ“ I Ε ο •p- JZ 1 φ >> ε -Ρ Π3 χ; CM tO Ο “D IO © Ο Φ Ρ χ: Ρ XZ (Ο Φ Ε Ρ to Η-» S- Ε « S- E ·© Ε I Ρ Ρ ε ε Ρ φ | Ρ ··- to I ρ •Ρ- Ο Xi Φ Ρ & Ρ φ ε id Ο φ ε CM φ ε ε 1 ο ε 1 £ Φ XJ Ρ r*~* Ρ to (0 Ρ «3 Π3 (Ο .Ω Ρ 1 -5 Α Φ , f— r— 1 1 Ρ— Ε 1 * Ό Ο XJ I 1 -Ο r““ Ε -Ο 5>» A © Xi >» Ο Ε CO *-* ρ- *—* ri cm Ο I 1 Ο Ο ε Ο -Ε Ρ Φ * 1 * I Ο Η«ι CM 1 ι— Ρ I Φ Ρ~ Ρ Ρ <Ο CM to CM 1 Α U»/ Si to Α φ t— ez * φ 3 α. »“ *-ν* * '-I-’ α Ο to 1 ε 1 toR ε >> o to ε ω φ * I to 1 to Α ο Ρ Ε c 1 * 'f- Ρ^ χζ r* ο V“ o <σ φ Φ ro -σ o to ro Ό >> ο 1 A «3 φ ^» φ ε Ρ |ν» P 1 ΧΖ ρν Ο Φ Φ C Ε ο α_ * 1 c ρ 1 Ρ ο •ι- Ε < c η ο I»* Ο Ε φ ι— CM 3 Φ γ- CM φ -C ο o r** I 1 I Ο ε 1 'ί-' tn P I '-Η ε ο Ρ 1 X 1 | to ο to 3 ο γ— 1 r— Q. Ο I ’Γ“ Ρ*“» ι— tO ο to ο i Ε .1 r— >1 Ο 5h Φ Ε Ο Ο © £> Λ Ρ • C ο Ε 4— Ο ε e e Ο t XZ C 3 E § φ ο φ •ι— ?Η Ρ Φ P o r- Φ Ζ ro Ρ Φ CQ £ Ε Ρ Ρ Ε © Φ Ε © XZ C α. φ Ρ 1 P 03 £Χ Ε ο. Κ- pH Σ Ο X © ο ο ζ © 3UT. Ο. Ε ©. I Φ f Φ ι U I ι 5h I I Ε 1 Φ Φ <± JZ Μ- Η-» *4 tO © CM Ό Μ· CL -£= Μ* XZ UJ Ρ Ε (Ο Ρ ν> Φ Γ“ Ο. ε «3 X Ld © Φ ε 1 Φ 1 E •t— pH ε o XJ X Φ I •t— N Zh o A pH ι X 1 E 1 ro Φ 1 pH I o P*% Φ pH «Ο Φ H-> A pH Φ •r· Φ X3 Φ Φ E c o rd Φ XJ Φ •r~ ε P o N (0 Ο c to T3 <0 Φ t C O tO Η-* Ε o Vv* o X H P to Φ N uv· Ο XJ I o E o o 1 1 xa C o Φ Ν Φ >) to N Φ XJ N Φ X3 E •r— Φ N ε ε ε XZ t sz ε Φ E H O Φ XJ £ -Q ε E Φ to c Φ XZ E Φ r-— P © •r~ •r~ Φ χ -Q χ Ε Φ xa X to O xi X A CL E X Ό X J3 ο •ρρ ο Ρ P •r“ o E 1 •P” © to Φ XJ o O © Χ3 Φ Q. XJ P to XJ XZ >> E Ί3 φ Ο φ Ρ Φ © Φ Φ 1 © Φ rd © JZ Φ T3 Φ o Ε Ε Ε ι -E E E P E E E 7^— (d E ;*» E E Ο XS Ο Χ3 o XJ O t Φ XJ O A © E O. XZ O XJ I >7 Ο >> t XZ P t «— >> P 1 rd 1 >, to χζ to r- © XZ to o Q. to 1 © Φ to E to _E 1 to 1 Α >> t r>v Φ rd 1 O pH P I P 1 ro Ε Ε ez to t? E E A JZ E E XJ © 1 E Φ E E Φ Ρ φ pH P Φ to o P Φ •r- Η-» xa Φ P Φ P Ρ φ Ρ rd © Φ P P— Φ P E rd o P 1 P Φ Ο. Ρ CL «-Η P CL rd © P CL rd o. ex Xi Q. P φ 1 Φ A to 1 Φ P~ >> I Φ E o * Φ o Φ ί χζ XI .Ε r~ Ο- X3 XZ A © xa _E O E to XZ r·» XZ J3 ο ο Ο I Ο O o pH o O 4- o. o * o o r~ E p—» I © r— p— o to r— to I-· © ”© >> Ο- © Η-» A © 3 P~ P“ © A © A >) to Zb Ο to. >> >» to to >» ω © A ?? to to © © P © c o. © © © ί---1 (Ο Φ © rd pH o o to pH © J pH * rH <0 © Γ- © ε >> r— © P E »“ © XZ pH o © r— © Up» o © A '-ν’ CL A P Φ r- Ή 1 Η-» A to rd E P*^ r—· to 3 © rd Φ x: rd »*> (Ο CL 1 Ο- O Φ | CL vi 1 Ο- ε rd P Ο- X CL 1 ο ο Ο 3 o © o Ο •P“ Η-» Ο o O o Ε Ε Ε cd >> E >> E E O o >> E P E ε CL ο CL >-r^ XZ CL © O CL 1 M E Ο- 3 Ο- o ο 3 Ο © P O P pH p— O Z E P Ο CQ Ο E E N Φ φ Φ XZ Φ Λ P“ P“ CQ *υ Lu Tj l·- E £ © S A o © z xa £ © C © I >» 1 1 Φ 1 I rd 1 5-» 1 1 t σ Ό· σ «d- XI t? <3- CM ©. © <3- © Ό © Φ XI 4- tn xz 23 57 Dosage Forms The antidepressant agents of this invention can be administered as treatment for psychiatric depressions of the reactive and endogenous types by any means that produces contact of the active agent with the agent's site of action in the body of a mammal. In addition to their antidepressant activity they also have a beneficial sedative action. They can be administered by any conventional means available for use in conjunction with pharmaceuticals; either as individual therapeutic agents or in a combination of therapeutic agents. They can be administered alone, but are generally administered with a pharmaceutical carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice.

The dosage administered will, of course, vary depending upon known factors such as the pharmacodynamic characteristics of the particular agent, and its mode and route of administration; age, health, and weight of the recipient; nature and extent of symptoms, kind of concurrent treatment, frequency of treatment, and the effect desired. Usually a daily dosage of active ingredient can be about 0.01 to 50 milligrams per kilogram of body weight. Ordinarily 0.05 to 40, and preferably 0.1 to 20 milligrams per kilograms per day given in divided doses 2 to 4 times a day or in sustained release form is effective to obtain desired results.

Dosage forms (compositions) suitable for internal administration contain from about 2 milligrams to about 10 milligrams of active ingredient per unit. In these pharmaceutical compositions the active ingredient will ordinarily be present in an amount of about 0.01—90% by weight based on the total weight of the composition.

The active ingredient can be administered orally in solid dosage forms, such as capsules, tablets, and powders, or in liquid dosage forms, such as elixirs, syrups, and suspensions; it can also be administered - 19 42357 parenterally, in sterile liquid dosage forms; or rectally in the form of suppositories.

Gelatin capsules contain the active ingredient and powdered carriers, such as lactose, sucrose, mannitol, starch, cellulose derivatives, mag5 nesium stearate or stearic acid. Similar diluents can be used to make compressed tablets. Both tablets and capsules can be manufactured as sustained release products to provide for continuous release of medication over a period of hours. Compressed tablets can be sugar coated or film coated to mask any unpleasant taste and protect the tablet from the atmosphere, or enteric coated for selective disintegration in the gastrointestinal tract.

Liquid dosage forms for oral administration can contain coloring and flavoring to increase patient acceptance.

In general, water, a suitable oil, saline, aqueous dextrose (glu15 cose), and related sugar solutions and glycols such as propylene glycol or polyethylene glycols are suitable carriers for parenteral solutions. Solutions for parenteral administration contain preferably a water soluble salt of the active ingredient, suitable stabilizing agents, and if necessary, buffer substances. Antioxidizing agents such as sodium bi20 sulfite, sodium sulfite, or ascorbic acid either alone or combined are suitable stabilizing agents. Also used are citric acid and its salts and sodium EDTA (ethylenediaminetetraacetic acid). In addition parenteral solutions can contain preservatives, such as benzalkonium chloride, methyl- or propyl-paraben, and chlorobutanol.

Suppositories contain the active ingredient in a suitable oleaginous or water-soluble base. The oleaginous class includes cocoa butter and fats with similar properties: the water-soluble class includes polyethylene glycols.

Suitable pharmaceutical carriers are described in Remington's Pharmaceutical Sciences, E.U. Martin, a standard reference text in this - 20 42357 field.

Useful pharmaceutical dosage-forms for administration of the compounds of this invention can be illustrated as follows: Capsules Capsules can be prepared by filling standard two-piece hard gelatin capsules with the following mixture using conventional encapsulating equipment: Active ingredient 5 mg. Lactose 125 mg. Talc 12 mg. Magnesium stearate 3 mg. Capsules A mixture of active drug in soy bean oil is prepared and injected by means of a positive displacement pump in gelatin to form soft gelatin capsules containing 5 mg. of the active ingredient. The capsules are washed in petroleum ether and dried.

Tablets Tablets can be prepared by conventional procedures so that each unit will contain: Active ingredient 5 mg. Spray dried lactose 150 mg. Microcrystalline cellulose 35 mg. Magnesium stearate 3 mg.

Patenteral Parenteral composition suitable for intra muscular administration is prepared so that each ml. contains: Active ingredient 5 mg.

Sodium carboxy methyl cellulose .75% Polysorbate 80 0.04% - 21 43357 Benzyl alcohol 0.9% Sodium chloride 0.9% Water for injection Q.S. 1 ml Suspension An aqueous suspension is prepared for oral administration so that each 5 mis. contain: Active ingredient 5 mg.

Methylcellulose 5% Carboxy methyl cellulose 5% Syrup 30% Polysorbate 80 0.2% Sodium saccharin 2 mg.

Cherry flavor 0.1% Sodium benzoate 5 mg.

Mater Q.S. 5 ml.

A standard procedure for detecting and comparing the anti-depressant activity of compounds in this series for which there is good correlation with human efficacy is the prevention of tetrabenazine-induced sedation and depression in mice. (Everett, The Dopa Response Potentiation Test and Its Use in Screening for Antidepressant Drugs, pp. 164—167 in Antidepressant Drugs {Proceedings of the First International Symposium}, S. Garattini and Μ. N. G. Dukes, eds., 1967.).

Groups of 10 Carworth CFiS female mice, 18—21 g. each, were fasted 1.5 hours and were intubated with antagonist compounds at oral doses of 0, 5, 25, and 125 mg/kg or 0, 1, 3, 9, 27, and 81 mg/kg in 0.20 ml. of 1% Methocel (registered Trade Mark for a proprietary methylcellulose).

The mice were challenged 30 minutes later with tetrabenazine (as the methane-sulfonate), 32 mg/kg intraperitoneally (dissolved in 0.20 nil, 0.05M KC1 at pH 2.0). One hour after antagonist (30 minutes after tetra30 benazine), the mice were examined for signs of exploratory activity and - 22 42357 ptosis (eyelid closure). Normal exploratory activity (relief from sedation) was recorded when a mouse lifted by the tail from a group of 10 in a testing box and placed on a stainless steel testing box lid (12,5 x 8 with 0.33“ mesh) either turned its head horizontally 30° in both directions or moved to the edge of the screen within 10 seconds after being placed on the screen. Relief from ptosis was recorded when exactly two seconds after placing the mouse facing the observer, lid closure was less than 50% in both eyes. The following table gives results. - 23 42357 Antagonism of Tetrabenazine-Induced Depression in Mice Orally at T Hour Post-Drug Compound ED5o(mg/kg) For Prevention of Rt, R3 Exploratory Loss Ptosis H -CH2CH2N(CH3)2 2.3 0.4 H -ch2ch2nh2.hci 0.10 0.09 H -CH2CH2CH2N(CH3)2 15.6 9.0 H -ch2ch2nhch3 1.9 0.33 Cl -CH2CH2N(CH3)2 11.2 7.2 H -CH2CH2N(CH3)2.HC1 1.4 0.5 H -ch2ch2nh2 0.25 0.09 H -ch2ch2£ 10.4 4.8 H -ch2ch2/ Jl 3.0 1.0 H -ch2ch2/ 4.7 1.7 H -CH2CH2N{CH(CH3)2}2 5.2 3.3 amitriptyline (Elavil) 4.7 1.7 imipramine (Tofranil; registered Trade Mark)3.0 2.5 NOCH2CH2N(CH3)2.HC1 Judd, U. S. Patent No. 3,349,128

Claims (21)

1. CLAIMS :1. Compounds of the formula 5 X and Y — H, F, Cl, Br, C 3 —Cq alkyl, C|—Cq alkoxy, CF 3 , CH 3 S, CH 3 SO 2 or S0 2 N(CH 3 ) 2 , provided that at least one is H; Z * Cz—C 3 alkylene; Ri and R 2 independently = H, Cj—Cq alkyl, or together with the nitrogen atom to which they are attached can form a ring; in which W is —(CH 2 )„-, —(CH 2 )s—, or _(CH 2 ) z O(CH 2 ) 2 — and their pharmaceutically suitable salts. ' '

2. Compounds of claim 1 in which Ri and R 2 = H or CH 3 . 15

3. Compounds of claim 1 in which X and Y = Η, Z = ethylene, and Ri and R 2 = H or CH 3 .

4. The compound of claim 3 in which Ri and R 2 = H.

5. The compound of claim 3 in which R x = H and R 2 = CH 3 .

6. The compound of claim 3 in which Ri and R 2 = CH 3 . 20

7. Compounds of claims 3—6 in the form of their hydrochloride salts.

8. Compounds of claim 1 as hereinbefore specifically disclosed excepting the compounds of claim 3—6.

9. A pharmaceutical composition comprising a suitable pharmaceutical carrier and a compound of claim 1. - 25 42357

10. A pharmaceutical composition comprising a suitable pharmaceutical carrier and a compound of claim 2.

11. A pharmaceutical composition comprising a suitable pharmaceutical carrier and a compound of claim 3. 5

12. A pharmaceutical composition comprising a suitable pharmaceutical carrier and a compound of claim 4.

13. A pharmaceutical composition comprising a suitable pharmaceutical carrier and a compound of claim 5.

14. A pharmaceutical composition comprising a suitable pharmaceut10 ical carrier and a compound of claim 6.

15. A pharmaceutical composition comprising a suitable pharmaceutical carrier and a compound of claim 7.

16. The composition of any of claims 9—15 in unit dosage form, each unit dose containing 2—10 mg of said compound. 15

17. A method of alleviating depression in a mammal other than man which comprises administering to the mammal an effective antidepressant amount of a compound of any of claims 1—8.

18. A process for preparing a compound of claim 1 which comprises reacting a corresponding compound of the formula: where M is an alkali metal; with a compound of the formula: Ri Hal—Z—Ν<ζ R 2 where Hal is a halogen and Ri and R2 are as defined in claim 1 in an inert anhydrous solvent at a temperature of 10—50°C. - 26 42357

19. A process for preparing a compound of claim 1 wherein Z =-CH 2 CH 2 —, which comprises reacting a corresponding compound of the formula: N-0-CH 2 CH 2 -0-Ts 5 with a compound of the formula: Ri ΗΝ<ζ R 2 wherein Ri and R 2 are as defined in claim 1.

20. A process for preparing a compound of claim 1 which comprises reacting a corresponding compound of the formula: OCH 3 0CH 3 ; with a compound of the formula: R z 15 wherein Rj and R 2 are as defined in claim 1 in the presence of pyridine.

21. The processes of claims 18, 19 or 20 substantially as hereinbefore described with reference to the Examples.