IE39670L - Fixing an enzyme on a support. - Google Patents
Fixing an enzyme on a support.Info
- Publication number
- IE39670L IE39670L IE741730A IE173074A IE39670L IE 39670 L IE39670 L IE 39670L IE 741730 A IE741730 A IE 741730A IE 173074 A IE173074 A IE 173074A IE 39670 L IE39670 L IE 39670L
- Authority
- IE
- Ireland
- Prior art keywords
- enzyme
- support
- activated
- fixing
- benzene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
Landscapes
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1450976 Insolubilized enzymes RHONE PROGIL SA 19 Aug 1974 [22 Aug 1973] 36399/74 Heading C3H In fixing an enzyme on a support by covalent bonding, the enzyme is reacted with a support possessing functional groups capable of reacting with the groups by which the enzyme can be fixed, in the presence of an anhydrous organic medium that does not dissolve the enzyme of the support, at a temperature of from 30 to 120 C. for, say, from 30 minutes to 2 hours. The reaction may be carried out under slightly increased pressure. The organic medium is exemplified by hexane, benzene, toluene, xylene, chloroform, carbon tetrachloride, dichloroethane, trichloroethane, trichloroethylene, perchloroethylene, dioxane, tetrahydrofuran and dimethylsulphoxide. The support may be of brick, silica, alumina, clay, sand, agarose, starch, polydextran, cellulose, polybutadiene, polystyrene, methacrylic acid copolymer, or a copolymer of maleic anhydride and ethylene. Examples 1 to 3 are directed to fixing pectinase, papain and trypsin, respectively, on activated brick particles of diameter between 0À5 and 0À8 mm. dispersed in boiling benzene or hexane. The brick particles were activated in a preliminary treatment with sulphuryl chloride. Example 4 relates to the fixing in benzene of ribonuclease on polybutadiene activated by reaction with acetyl chloride. According to Example 5 glycoamylase is fixed on particles of silica, which had been activated by the grafting of a silane with expoxy function, in boiling chloroform. In comparative experiments the bonding was effected in an aqueous medium.
[GB1450976A]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7330413A FR2278702A1 (en) | 1973-08-22 | 1973-08-22 | PROCESS FOR FIXING ENZYMES ON SUPPORTS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE39670L true IE39670L (en) | 1975-02-22 |
| IE39670B1 IE39670B1 (en) | 1978-12-06 |
Family
ID=9124191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE1730/74A IE39670B1 (en) | 1973-08-22 | 1974-08-20 | Process for fixing enzymes on supports |
Country Status (17)
| Country | Link |
|---|---|
| JP (1) | JPS511793B2 (en) |
| BE (1) | BE819025A (en) |
| BR (1) | BR7406867D0 (en) |
| CA (1) | CA1032486A (en) |
| CH (1) | CH584718A5 (en) |
| DE (1) | DE2439923B2 (en) |
| DK (1) | DK139585B (en) |
| FI (1) | FI52984C (en) |
| FR (1) | FR2278702A1 (en) |
| GB (1) | GB1450976A (en) |
| IE (1) | IE39670B1 (en) |
| IT (1) | IT1018768B (en) |
| LU (1) | LU70763A1 (en) |
| NL (1) | NL7411086A (en) |
| NO (1) | NO143537C (en) |
| SE (1) | SE423408B (en) |
| SU (1) | SU578893A3 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2703703A1 (en) * | 1977-01-29 | 1978-08-03 | Dynamit Nobel Ag | CARRIER-BONDED ACYLASES |
| EP0003923B1 (en) * | 1978-02-16 | 1982-10-20 | Rhone-Poulenc Specialites Chimiques | Support-enzyme complexes and method for preparing them |
| FR2447400A2 (en) * | 1979-01-23 | 1980-08-22 | Rhone Poulenc Ind | Enzymes immobilised on inorganic supports - by attaching to bridging molecule bonded to support by an ester linkage |
| JPS61236314A (en) * | 1985-04-08 | 1986-10-21 | 株式会社竹中工務店 | Wire type cable rack mounting construction |
| SE459288B (en) * | 1987-08-24 | 1989-06-19 | Carl Eric Ohlson | SET ON REVIEW OF ROENTGEN FILMS AND SIMILAR AND REVIEW CABINET FOR SUCH FILM REVIEW |
-
1973
- 1973-08-22 FR FR7330413A patent/FR2278702A1/en active Granted
-
1974
- 1974-07-30 IT IT52355/74A patent/IT1018768B/en active
- 1974-08-19 GB GB3639974A patent/GB1450976A/en not_active Expired
- 1974-08-19 FI FI2445/74A patent/FI52984C/fi active
- 1974-08-20 DK DK443074AA patent/DK139585B/en unknown
- 1974-08-20 BR BR6867/74A patent/BR7406867D0/en unknown
- 1974-08-20 LU LU70763A patent/LU70763A1/xx unknown
- 1974-08-20 BE BE147766A patent/BE819025A/en not_active IP Right Cessation
- 1974-08-20 SE SE7410576A patent/SE423408B/en unknown
- 1974-08-20 NO NO742985A patent/NO143537C/en unknown
- 1974-08-20 NL NL7411086A patent/NL7411086A/en not_active Application Discontinuation
- 1974-08-20 DE DE19742439923 patent/DE2439923B2/en active Granted
- 1974-08-20 IE IE1730/74A patent/IE39670B1/en unknown
- 1974-08-20 CA CA207,447A patent/CA1032486A/en not_active Expired
- 1974-08-21 CH CH1142474A patent/CH584718A5/xx not_active IP Right Cessation
- 1974-08-21 SU SU7402058144A patent/SU578893A3/en active
- 1974-08-21 JP JP49096035A patent/JPS511793B2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CA1032486A (en) | 1978-06-06 |
| NO742985L (en) | 1975-03-24 |
| DE2439923B2 (en) | 1976-09-02 |
| NO143537B (en) | 1980-11-24 |
| FI52984C (en) | 1978-01-10 |
| FI52984B (en) | 1977-09-30 |
| LU70763A1 (en) | 1975-06-11 |
| FR2278702B1 (en) | 1976-12-03 |
| CH584718A5 (en) | 1977-02-15 |
| SE7410576L (en) | 1975-02-24 |
| DE2439923A1 (en) | 1975-03-20 |
| FR2278702A1 (en) | 1976-02-13 |
| DK139585B (en) | 1979-03-12 |
| IE39670B1 (en) | 1978-12-06 |
| SE423408B (en) | 1982-05-03 |
| JPS511793B2 (en) | 1976-01-20 |
| JPS5049488A (en) | 1975-05-02 |
| IT1018768B (en) | 1977-10-20 |
| FI244574A (en) | 1975-02-23 |
| DK139585C (en) | 1979-09-03 |
| BR7406867D0 (en) | 1975-06-17 |
| BE819025A (en) | 1975-02-20 |
| SU578893A3 (en) | 1977-10-30 |
| DK443074A (en) | 1975-04-28 |
| GB1450976A (en) | 1976-09-29 |
| NO143537C (en) | 1981-03-04 |
| NL7411086A (en) | 1975-02-25 |
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