IE20090875A1 - A peptide and use thereof - Google Patents

A peptide and use thereof

Info

Publication number
IE20090875A1
IE20090875A1 IE20090875A IE20090875A IE20090875A1 IE 20090875 A1 IE20090875 A1 IE 20090875A1 IE 20090875 A IE20090875 A IE 20090875A IE 20090875 A IE20090875 A IE 20090875A IE 20090875 A1 IE20090875 A1 IE 20090875A1
Authority
IE
Ireland
Prior art keywords
seq
peptide
amino acid
acid sequence
tlr4
Prior art date
Application number
IE20090875A
Inventor
Barry Noel Harrington
Tatyana Sergeevna Lysakova
Brian Keogh
Andrew Graham Bowie
Original Assignee
Trinity College Dublin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trinity College Dublin filed Critical Trinity College Dublin
Priority to IE20090875A priority Critical patent/IE20090875A1/en
Publication of IE20090875A1 publication Critical patent/IE20090875A1/en

Links

Abstract

A peptide, derived from the vaccinia virus protein A46, for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID NO. 4, SEQ ID NO 55, SEQ ID NO 68, SEQ ID NO. 69, SEQ ID NO 70., SEQ ID NO 71, SEQ ID NO 72, SEQ ID NO 79, SEQ ID NO 82, SEQ ID NO 85, SEQ ID NO 88, SEQ ID NO 91, SEQ ID NO 94, SEQ ID NO 97, SEQ ID NO 100, SEQ ID NO 103, SEQ ID NO 106, SEQ ID NO 109, SEQ ID NO 112, or SEQ ID NO 115. The peptide may comprise a delivery sequence such as a cationic peptide. <Figure 3>

Description

This invention relates to a peptide derived from a vaccinia virus protein. In particular the invention relates to a peptide derived from the vaccinia virus protein A46.
Pattern recognition receptors (PRRs) are critical for the ability of the innate immune response to detect pathogens. Examples of classes of PRRs include Toll-like receptors (TLRs), RlG-l-like receptors (RLRs) and NOD-like receptors (NLRs). TLRs recognize pathogen-associated molecular patterns (PAMPs) on microorganisms, leading to the activation of signaling pathways and subsequent altered gene expression. This leads to the production of anti-microbial effector cytokines such as the proinflammatory cytokines interleukin-1 (IL-1) and tumour necrosis factor ft (TNPft). and the type I interferons (IFNs) IFNa and IFNp. Specific TLRs have been shown to detect particular PAMPs, for example TLR2 recognises certain bacterial lipopeptides. I LR3 recognises double-stranded (ds) RNA, TLR4 responds to lipopolysaccharide (LPS). TLR7 or TLRS to ssRNA. and TLR9 to dsDNA containing CpG motifs (Akira et ah 2006).
TLR4 is of particular interest here, and is one of the most important and studied of the TLRs. given its role in mediating the many effects of LPS on cells. Furthermore, TLR4 can respond to a variety of other cellular insults and endogenous danger signals, making it an attractive drug target for therapeutic intervention in the large range of diseases now appreciated to involve inappropriate or aberrant activation of innate immune signaling. Inhibitors of specific innate immune signaling pathways are an attractive strategy in certain disease contexts in order to disable pathways contributing to disease while maintaining some redundant pathogen detection systems. As such, a specific inhibitor of TLR4 would be a valuable asset.
An important example of a role for TLRs in disease processes is that of inflammation. Inflammation is a complex process underlying a large number of acute and chronic diseases such as sepsis, rheumatoid arthritis, multiple sclerosis and colitis. Most therapeutic approaches to blocking inflammation target individual effector cytokines such as TNF, often with good effect. There is now strong evidence that TLRs have a crucial role in initiating both pathogen-induced and sterile inflammation, and as such TLRs provide the initial trigger which ultimately leads to the production oil si^j^^^^^^vje^^rNedh |006). Therefore, targeting the initial intracellular TLR signalling cascades directly is uftimat :ly likely tp..hfi»aia.more fjTfcfftiw rare» :::::::: [ JKL approach.
IE 0 9 0 8 7 5 -2TLR4 activators cause it to homodimerise, via its intracellular Toll-IL-IR (TIR) domain. This leads to the engagement of TIR domain-containing adaptor proteins with the receptor complex, namely myeloid differentiation factor 88 (MyD88), MyD88 adaptor-like (MaL also called 1IRAP). TIR domain-containing adaptor inducing IFNp (TRIF), and TRIF-related adaptor molecule (TRAM). Together, MyD88 and Mai mediate a signaling pathway leading to NFkB and mitogen-activated protein kinase (MAPK) activation, while TRAM and TRIF control a pathway leading to NFkB and IFN regulatory factor (IRF) activation (O’Neill and Bowie. 2007). Oilier TLRs use different repertoires of TIR adaptors to signal, for example TLR2 utilises MyD88 and MaL TLR3 utilises only TRIF and TLR9 utilises only MyD88 (O'Neill and Bowie, 2007).
Two vaccinia virus proteins, A46R and A52R, have been identified which can modulate intracellular signalling by TLRs and thus inhibit the TLR-initiated immune response. A52R potently blocks I LR-induced activation of the transcription factor NFkB (a well-known lead drug target) by interacting with the TLR signalling molecule IRAK2 (Harte el al, 2003; Keating el al, 2007). A46 has distinct targets, and can suppress TLR signaling axes utilising MyD88. MaL IRIF or TRAM, leading to inhibition of MAP kinase, NFkB and 1RF3 activation (Stack et al, 2005). I his is because A46 has a TIR domain, which allows it to bind to mammalian TIR adaptors (Stack et al, 2005).
Statements of Invention We describe a peptide derived from the vaccinia virus protein A46 and use thereof in methods for suppressing a pro-inflammatory response mediated by TLR4. The peptide may comprise the consensus sequence KLIL (SEQ ID No. 69), for example the peptide may be selected from one or more ofthe following (in which X indicates the presence of the amino acid residue): AMINO ACID RESIDUE SEQ ID NO. K Y s F K L 1 L A E Y Peptide (no delivery sequence) Peptide with 9R delivery sequence at the C terminus Peptide with 9R delivery sequence at the N terminus X X X X 69 74 78 X X X X X 68 73 62 X X X X X X 55 56 57 X X X X X X X 79 80 81 X X X X X X X X 70 42 75 IE 0 9 0 8 7 5 X X X X X 82 83 84 X X X X X X 85 86 87 X X X X X X X 88 89 90 X X X X X X X X 91 92 93 X X X X X X X X X 94 95 96 X X X X X X 97 98 99 X X X X X X X 100 101 102 X X X X X X X X 103 104 105 X X X X X X X X X 106 107 108 X X X X X X X X X X 109 110 1 1 1 X X X X X X X 112 113 1 14 X X X X X X X X 115 116 1 17 X X X X X X X X X 71 41 76 X X X X X X X X X X 72 40 77 X X X X X X X X X X X 4 20 38 In one embodiment the peptide may comprise the consensus sequence FKLIL (SEQ II.) No. 68) In accordance with the invention there is provided a peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID No. 69.
The invention further provides a peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID No. 68.
The invention also provides a peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID NO. 4, SEQ ID NO 55, SEQ ID NO 68, SEQ ID NO. 69. SEQ ID NO 70, SEQ ID NO 71, SEQ ID NO 72, SEQ ID NO 79, SEQ ID NO 82. SEQ ID NO 85. SI Q ID NO 88, SEQ ID NO 91, SEQ ID NO 94, SEQ ID NO 97, SEQ ID NO 100. SEQ ID NO 103. SEQ ID NO 106, SEQ ID NO 109, SEQ ID NO 112. or SEQ ID NO 115.
The amino acid sequence may be in the L-forni. Alternatively, the amino acid sequence may be ill the D-lbrni.
The peptide may comprise a delivery sequence. The delivery sequence may be a cationic peptide. The delivery sequence may be between 8 and 16 amino acids in length. I he delivery sequence may comprise the amino acid sequence of SEQ ID NO. 33, SEQ ID NO. 34. or SEQ ID NO. 35.
I lie delivery sequence may be attached to the C terminus of the peptide. The peptide may comprise an amino acid sequence selected from the group comprising: SEQ ID No. 20. SEQ ID IB 0 9 08 7 5 -4No. 40. SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 56, SEQ ID No. 73, SEQ ID No. 74. SEQ ID No. 80, SEQ ID No. 83, SEQ ID No. 86, SEQ ID No. 89, SEQ ID No. 92, SEQ ID No. 95. SEQ ID No. 98. SEQ ID No. 101, SEQ ID No. 104, SEQ ID No. 107, SEQ ID No. 110. SEQ ID No. 113. and SEQ ID No. 116.
I he delivery sequence may be attached to the N terminus of the peptide. The peptide may comprise an amino acid sequence selected from the group comprising: SEQ ID No. 38, SEQ ID No. 57. SEQ ID No. 62, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78. SEQ ID No. 81. SEQ ID No. 84, SEQ ID No. 87, SEQ ID No. 90, SEQ ID No. 93, SEQ ID No. 96, SEQ ID No. 99. SEQ ID No. 102, SEQ ID No. 105, SEQ ID No. 108, SEQ ID No. 111. SEQ ID No. 114. and SEQ ID No. 117, The invention also provides a peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID No. 20, SEQ ID No. 38. SEQ ID No. 40. SEQ ID No. 41. SEQ ID No. 42, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 73. SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 80, SEQ ID No. 81. SEQ ID No. 86. SEQ ID No. 87. SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 95. SEQ ID No. 96, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 110, SEQ ID No.l 11, SEQ ID No.116. or SEQ ID No.117 The invention further provides a peptide comprising the amino acid sequence of SEQ ID NO. 20 or a fragment, analogue or derivative thereof.
I he invention also provides a peptide comprising the amino acid sequence of SEQ ID NO. 62 or a fragment, analogue or derivative thereof.
The invention further still provides a peptide comprising the amino acid sequence of SEQ ID NO. 38 or a fragment, analogue or derivative thereof.
The invention further provides a peptide comprising the amino acid sequence of SEQ ID NO. 73 or a fragment, analogue or derivative thereof.
IE 0 9 0 8 7 5 5 The invention also provides a peptidomimetic for inhibiting Toll-like receptor 4 (TLR4) signalling based on a peptide as described herein.
The invention further provides a pharmaceutical composition comprising a peptide or a peptidomemetie as described herein and a pharmaceutically acceptable excipient.
I he invention also provides for the use of a peptide or a peptidomimetic or a pharmaceutical composition as described herein to inhibit Toll-like receptor 4 (TLR4) signalling. The TLK4 signalling may be activated by a pathogen or pathogen component leading to a cytokine response. The TLR4 signalling protein activated may be one or more of NFkB. IkBoc IRE3 and p38. I he pathogen may be a bacterium or a bacterial component such as lipopolysaeeharide.
The invention further provides a method of treatment or prophylaxis of a TLR4-a$soeialed disease comprising the step of administering an effective amount of a peptide aor a peptidomimetic or a pharmaceutical composition as described herein to a subject. The disease may be a disease of the immune system and/or is an inflammatory disease. For example, the disease may be one or more of: sepsis, rheumatoid arthritis, colitis, multiple sclerosis, irritable bowel disease, cancer, sterile inflammation, pathogen-associated inflammation, kidney ischemia/ieperfusion injury, liver ischemia/reperfusion injury, plaque development in atherosclerosis-prone subjects, and acute lung injury.
The invention also provides a method of inhibiting TLR4-induced cytokine responses comprising the step of administering an effective amount of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41. SEQ ID No. 42. SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 68, SEQ ID No 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73. SEQ ID No. 74. SEQ ID No. 75, SEQ ID No. 76. SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79. SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82. SEQ ID No, 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97. SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102. SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106. SEQ ID No. 107. SEQ ID No. 108. SEQ ID No. 109,SEQ ID No. 110, SEQ ID No. Ill,SEQ ID No. 112. SEQ ID No. 113. SEQ ID No. 114, SEQ ID No. 115. SEQ ID No. 116, or SEQ ID No. 117 to a subject.
IE 0 9 0 8 7 5 -6I he invention further provides a method of inhibiting TLR4 induced responses comprising the step of administering an effective amount of a peplidomimetic based on the amino aeid sequence of SEQ II) No. 4. SEQ ID No. 55, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70. SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 79, SEQ ID No. 82, SEQ ID No. 85. SEQ ID No. 88. SEQ ID No. 91, SEQ ID No. 94, SEQ ID No. 97, SEQ ID No. 100, SEQ ID No. 103, SEQ ID No. 106. SEQ ID No. 109, SEQ ID No. 112, or SEQ ID No. 115 to a subject.
The invention also provides a method of inhibiting TLR4 induced responses comprising the step of administering an effective amount of a pharmaceutical composition comprising a peptide having the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38. SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62. SEQ ID No. 68. SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71. SEQ ID No. 72. SEQ ID No. 73. SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78. SEQ ID No.79. SEQ ID No. 80, SEQ ID No. 81. SEQ ID No. 82, SEQ ID No. 83. SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89. SEQ ID No. 90. SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94. SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100. SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105. SEQ ID No. 106. SEQ ID No. 107. SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. II I. SEQ ID No. 112, SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, SEQ ID No. 117. or peptidomimetic thereof and a pharmaceutically acceptable excipient to a subject.
The invention further provides a method of suppressing a pro-inflammatory immune response comprising the step of administering an effective amount of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40. SEQ ID No. 41. SEQ ID No. 42. SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 68. SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No, 73. SEQ ID No. 74. SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79. SEQ ID No. 80. SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84. SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87. SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90. SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No, 96. SEQ ID No. 97. SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102. SEQ ID No. 103. SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106. SEQ ID No. 107. SEQ IE 0 9 08 7 5 -7ID No. 108. SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. Ill, SEQ ID No. 112. SEQ ID No. 113. SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, SEQ ID No. 117, or peptidomimetic thereof lo a subject.
The invention also provides for the use of a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41. SEQ ID No. 42. SEQ IL) No. 55. SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81. SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97. SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102. SEQ ID No. 103. SEQ II) No. 104, SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107. SEQ ID No. 108. SEQ ID No 109. SEQ ID No. 110, SEQ ID No. Ill, SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116. or SEQ ID No. 117 to suppress an immune response wherein the immune response is mediated through the stimulation of TLR.4 leading to the activation of a MAP kinase, or at least one transcription factor selected from NF-κΒ and at least one IRF. The 1RF may be IRF3 or IRF7. fhe invention also provides for the use of a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20, SEQ ID No. 38. SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70. SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74. SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80. SEQ ID No. 8E SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87. SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92. SEQ II) No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97. SEQ ID No. 98. SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103. SEQ ID No. 104. SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108. SEQ ID No. 109. SEQ ID No. 110, SEQ ID No. 111. SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116, or SEQ ID No. 117 in the preparation of a medicament for down regulating a TLR4-mediated immune response. The immune response may be IE 0 9 0 8 7 5 -8mediated through the activation of at least one MAP kinase or a transcription factors selected from NF-icB and at least one IRF. The IRF may be IRF3 or IRF7.
The invention further provides for a pharmaceutical composition comprising a therapeutically effective amount of a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20. SEQ ID No. 38. SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56. SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71. SEQ ID No. 72, SEQ ID No. 73. SEQ ID No. 74, SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81. SEQ ID No. 82. SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88. SEQ ID No 89, SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93. SEQ ID No. 94. SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98. SEQ ID No. 99. SEQ ID No. tOO. SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104. SEQ ID No. 105. SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109. SEQ ID No. 110. SEQ ID No. Ill, SEQ ID No. 112, SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116. or SEQ ID No. 117 and a pharmaceutically acceptable diluent, excipient or carrier.
The invention also provides for a method of prophylaxis and/or treatment of an imnwnemediated condition comprising the step of administering an agent comprising a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38. SEQ ID No. 40. SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62. SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72. SEQ ID No. 73. SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79. SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83. SEQ ID No. 84. SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89. SEQ ID No. 90. SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95. SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110. SEQ ID No. 111. SEQ ID No. 112, SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, SEQ ID No. 117, or pcptidomimetic thereof to a subject wherein administration of the agent suppresses the activation ot a MAP kinase or the transcription factors NF-κΒ and at least one IRF. The IRF may be IRE3 or IRF7. The immune mediated disorder may be an undesirable or aberrant /£ 0 9 0 8 7 5 -9immune response triggered by the activation of TLR4. The immune response may be directed to a self antigen. The immune response may be physiologically normal but is undesirable.
The immune mediated condition may be one or more selected from the group comprising; multiple sclerosis, rheumatoid arthritis, Crohn’s disease, psoriasis, SLE, lupus, type I diabetes, colitis, inflammatory bowel disease, asthma, allergy diabetes mellitus, myasthenia gravis, systemic lupus erythematosis. autoimmune thyroiditis, dermatitis (including atopic dermatitis and eczematous dermatitis), Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, alopecia areata, allergic responses due to arthropod bite reactions, aphthous ulcer, iritis, conjunctivitis, keratoconjunctivitis, ulcerative colitis, cutaneous lupus erythematosus, scieroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, allergic encephalomyelitis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis. Wegener's granulomatosis, chronic active hepatitis. Stevens-Johnson syndrome, idiopathic sprue, lichen planus. Graves ophthalmopathy, sarcoidosis, primary biliary cirrhosis, uveitis posterior, interstitial lung fibrosis, Alzheimer’s disease and coeliac disease, or atopic disease.
I’hc immune-niediated condition may be an autoimmune disease. The autoimmune disease may be one or more selected from the group comprising: multiple sclerosis, rheumatoid arthritis. Crohn's disease, psoriasis, SLE, lupus, type 1 diabetes, colitis, inflammatory bowel disease, asthma and allergy.
The invention further provides a method for down regulating an immune response of a subject following tissue transplantion comprising the step of administering an agent comprising a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56. SEQ ID No. 57. SEQ ID No, 62, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71. SEQ ID No. 72. SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76. SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83. SEQ ID No. 84. SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88. SEQ ID No. 89. SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94. SEQ ID No. 95. SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103. SEQ ID No. 104, SEQ ID No. 105. SEQ ID No.
IE 0 9 0 8 7 5 - 10106. SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110. SEQ ID No. 111, SEQ ID No. 1 12. SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116. SEQ ID No. 117. or peptidomimetic thereof to a subject.
The invention also provides a method of modulating intracellular signalling mediated by 1LR4 comprising the step of administering a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42. SEQ ID No. 55. SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70. SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80. SEQ ID No. 81. SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ IDNo.85, SEQ ID No. 86. SEQ ID No. 87. SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93. SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97. SEQ ID No. 98. SEQ ID No. 99. SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103. SEQ ID No. 104. SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. 111. SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114. SEQIDNo. 115. SEQ ID No. 116, or SEQ ID No. 117 to a subject.
The invention further provides for the use of an A464 peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41. SEQ ID No. 42. SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 68. SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73. SEQ ID No, 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79. SEQ ID No. 80. SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90. SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97. SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102. SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106. SEQ ID No. 107. SEQ ID No. 108. SEQIDNo. 109, SEQ ID No. 110, SEQIDNo. Ill, SEQ ID No. 112. SEQIDNo. 113. SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, or SEQ ID No. 117 to modulate intracellular signalling mediated by TLR4 following the binding of a suitable agonist.
The invention also provides for a method for identifying a compound and/or substance suitable for modifying the biological activity of TLR4 comprising the steps of; IE 0 9 Ο 8 Ί 5 a) contacting a biological sample with a compound and/or substance to be tested in the presence of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38. SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55. SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62. SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71. SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76. SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80. SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85. SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94. SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98. SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. Ill, SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, or SEQ ID No. 117; bi assaying the biological sample for a biological response; and c) comparing the biological response of a sample contacted with a compound and/or substance in the presence of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40. SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56. SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72. SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88. SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110. SEQ ID No. Ill, SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116, or SEQ ID No. 117 to the biological response IE 0 9087 5 of a sample contacted with a compound and/or substance in the absence of the peptide. 1'he biological response may be one or more of MAP kinase activation, transcription factor activation and gene induction. The biological response may be inhibited by the presence of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40. SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56. SEQ ID No. 57. SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72. SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77. SEQ ID No. 78. SEQ ID No.79, SEQ ID No. 80. SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83. SEQ ID No. 84. SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88. SEQ ID No. 89. SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95. SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105. SEQ ID No. 106. SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110. SEQ ID No. 111. SEQ ID No. 112. SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116. or SEQ ID No. 117.
The biological sample may be cultured cells. The biological sample may be a non-human animal.
The invention also provides for the use of a compound and/or substance identified by the method described herein as an adjuvant and/or booster of an immune response.
The invention further provides for a vaccine comprising a compound and/or a substance identified by the method described herein. t he invention further still provides for a composition comprising a compound and/or a substance identified by the method described herein and a pharmaceutically acceptable excipient.
In one embodiment, we describe a peptide comprising 11 amino acids derived from the vaccinia virus (VACV) protein A46, that when fused to a peptide delivery sequence, inhibits both murine and human loll-1 ike receptor 4 (TLR4) responses. One embodiment of the peptide, termed A464. has the amino acid sequence KYSFKL1EAEYRRRRRRRRR (SEQ ID No. 20). The IE Ο 9 Ο 8 7 5 - 13peptide is specific for TLR4 since other TLRs are not affected by it. We have also demonstrated that a peptide comprising the amino acid sequence SFKL1L (SEQ ID No. 55) can inhibit TLR4. Furthermore, we have also demonstrated that A464 (SEQ ID No. 20) has efficacy in vivo, to inhibit LPS-induced cytokine responses in mice.
In one aspect, the invention provides a peptide derived from vaccinia virus prolein A46 for inhibiting Toll-like receptor 4 (TLR4) signalling. The peptide may comprise between 4 and 11 amino acids. The amino acid sequence of the peptide may be derived from the amino acid sequence of SEQ ID NO, 16. fhe peptide may comprise the amino acid sequence of SEQ ID NO. 3, SEQ ID NO. 4. SEQ ID NO. 9. SEQ ID NO. 10, SEQ ID No. 54, or SEQ ID NO. 55 or a fragment, analogue or derivative thereof. The amino acid sequence may be in the L-form, alternatively the amino acid sequence may be in the D-form. fhe peptide may comprise a delivery sequence. The delivery sequence may be a cationic peptide. The delivery sequence may be between 8 and 16 amino acids in length. The delivery sequence may comprise the amino acid sequence of SEQ ID NO. 33, SEQ ID NO. 34. or SEQ ID NO. 35. The delivery sequence may be attached to the C terminus of the peptide. The delivery sequence may be attached to the N terminus of the peptide.
The invention further provides a peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID NO. 20, SEQ ID NO. 36, SEQ ID NO. 38. or SEQ ID NO. 57 or a fragment, analogue or derivative thereof. lhe invention also provides for a peptide comprising the amino acid sequence of SEQ ID NO. 20 or a fragment, analogue or derivative thereof, a peptide comprising tbe amino acid sequence of SEQ ID NO, 36 or a fragment, analogue or derivative thereof, a peptide comprising the amino acid sequence of* SEQ ID NO. 38 or a fragment, analogue or derivative thereof, and a peptide comprising the amino acid sequence of SEQ ID NO. 57 or a fragment, analogue or derivative thereof.
In another aspect the invention provides a peplidomimetic for inhibiting Toll-like receptor 4 (TLR4) signalling based on a peptide as described herein.
IE Ο 9 Ο 8 7 5 - 14Further, the invention provides for a pharmaceutical composition comprising a peptide as described herein or a peptidomemetic as described herein and a pharmaceutically acceptable excipient.
I he invention also provides for the use of a peptide or a peptidomimetic or a pharmaceutical composition as described herein to inhibit Toll-like receptor 4 (TLR4) signalling. The TLR4 signalling may be activated by a pathogen or pathogen component leading to a cytokine response. The TLR4 signalling protein activated may be one or more of NFkB. ΙκΒα, IRF3 and p38. The pathogen may be a bacterium or a bacterial component such as lipopolysaccharidc (LPS).
The invention also provides for a number of methods including: A method of treatment or prophylaxis of a TLR4-associated disease comprising the step of adminislering an effective amount of a peptide or a peptidomimetic or a pharmaceutical composition as described herein to a subject. The disease may be a disease of the immune system and/or an inflammatory disease. The disease may be one or more of: sepsis, rheumatoid arthritis, colitis, multiple sclerosis, irritable bowel disease, cancer, sterile inflammation. pathogen-associated inflammation, kidney ischemia/reperfusion injury. liver ischemia/reperfusion injury, plaque development in atherosclerosis-prone subjects, and acute lung injury.
A method of inhibiting TLR4-induced cytokine responses comprising the step of administering an effective amount of a peptide comprising the amino acid sequence of SEQ ID NO 3, SEQ ID NO. 4. SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 20, SEQ ID NO. 36. SEQ ID NO. 38, SEQ ID NO. 54. SEQ ID NO. 55, or SEQ ID NO. 57 or a fragment, analogue or derivative thereof to a subject.
A method of inhibiting TLR4 induced responses comprising the step of administering an effective amount of a peptidomimetic based on the amino acid sequence of SEQ ID NO. 3. SEQ ID NO. 4. SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 20, SEQ ID NO. 36, SEQ ID NO. 38. SEQ ID NO. 54. SEQ ID NO. 55, or SEQ ID NO. 57 or a fragment, analogue or derivative (hereof to a subject. -15IE 0 9 0 8 7 5 A method of inhibiting TLR4 induced responses comprising the step of administering an effective amount of a pharmaceutical composition comprising a peptide having the amino acid sequence of SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 20, SEQ ID NO. 36, SEQ ID NO. 38, SEQ ID NO. 54, SEQ ID NO. 55, or SEQ ID NO. 57 or a fragment, analogue derivative or peptidomimelic thereof and a pharmaceutically acceptable excipient to a subject.
A method of suppressing a pro-inflammatory immune response comprising the step of administering an effective amount of a peptide comprising the amino acid sequence of SEQ ID No.20 or an analogue, derivative, fragment, variant or peptidomimetic thereof thereof to a subject.
A method of prophylaxis and/or treatment of an immune-mediated condition comprising the step of administering an agent comprising a peptide comprising the amino acid sequence of SEQ ID No.20 or an analogue, derivative, fragment, variant or peptidomimetic thereof to a subject wherein administration of the agent suppresses the activation of a MAP kinase or the transcription factors NF-κΒ and at least one 1RF. The 1RF may be IRF3 or 1RF7. The immune mediated disorder may be an undesirable or aberrant immune response triggered by the activation of IT.R4. The immune response may be directed to a self antigen. The immune response may be physiologically normal but undesirable.
The immune mediated condition may be one or more selected from the group comprising: multiple sclerosis, rheumatoid arthritis, Crohn’s disease, psoriasis, SLE, lupus, type I diabetes, colitis, inflammatory bowel disease, asthma, allergy diabetes mellitus. myasthenia gravis, systemic lupus erythematosis, autoimmune thyroiditis, dermatitis (including atopic dermatitis and eczematous dermatitis), Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren’s Syndrome, alopecia areata, allergic responses due to arthropod bite reactions, aphthous ulcer, iritis, conjunctivitis, keratoconjunctivitis, ulcerative colitis, cutaneous lupus erythematosus, scieroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, allergic encephalomyelitis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis. Wegener's granulomatosis, chronic active hepatitis, Stevens-Johnson syndrome, idiopathic sprue, lichen -16IE 0 9 0 8 7 5 planus, (naves ophthalmopathy, sarcoidosis, primary biliary cirrhosis, uveitis posterior, interstitial lung fibrosis, Alzheimer’s disease and coeliac disease, or atopic disease. l he inimune-mediated condition may be an autoimmune disease such as an autoimmune disease selected from one or more of the group comprising: multiple sclerosis, rheumatoid arthritis, Crohn's disease, psoriasis, SLE, lupus, type I diabetes, colitis, inflammatory bowel disease, asthma and allergy.
The invention further provides a method for down regulating an immune response of a subject following tissue transplantion comprising the step of administering an agent comprising a peptide comprising the amino acid sequence of SEQ ID No.20 or an analogue, derivative, fragment, variant or peptidomimetic thereof to a subject.
The invention also provides a method of modulating intracellular signalling mediated by TLR4 comprising the step of administering a peptide comprising the amino acid sequence of SEQ ID No.20 or a derivative, fragment, or variant thereof to a subject.
In a different aspect the invention provides for the use of a peptide comprising the amino aeid sequence ol'SEQ ID No.20 or a fragment, analogue or derivative thereof to suppress an immune response wherein the immune response is mediated through the stimulation of TLR4 leading to the activation of a MAP kinase, or at least one transcription factor selected from NF-κΒ and at least one I Rl-. The IRF may be IRF3 or IRF7. l he invention further provides for the use of a peptide comprising the amino acid sequence of SEQ ID No.20 or a fragment, analogue or derivative thereof in the preparation of a medicament for down regulating a TLR4-mediated immune response. The immune response may be mediated through the activation of at least one MAP kinase or a transcription factors selected from N F-κΒ and at least one IRF. The IRF may be IRF3 or IRF7. l he invention also provides a pharmaceutical composition comprising a therapeutically effective amount of a peptide comprising the amino acid sequence of SEQ ID No.20 or a fragment, analogue or derivative thereof and a pharmaceutically acceptable diluent, excipient or carrier. -17IE 0 9 0 8 7 5 The invention further provides for the use ofa peptide comprising the amino acid sequence of SEQ ID No.20 or a variant, derivative or fragment thereof to modulate intracellular signalling mediated by TLR4 following the binding of a suitable agonist.
In a further aspect, the invention provides a method for identifying a compound and/or substance suitable for modifying the biological activity of TLR4 comprising the steps of: (a) contacting a biological sample with a compound and/or substance lo be tested in the presence and absence of a peptide comprising the amino acid sequence of SEQ ID No. 20 or a fragment, analogue or derivative thereof; (b) assaying the biological sample fora biological response; and (c) comparing the biological response of a sample contacted with a compound and/or substance in the presence of a peptide comprising the amino acid sequence of SEQ ID No. 20 or a fragment, analogue or derivative thereof to the biological response ofa sample contacted with a compound and/or substance in the absence of a peptide comprising the amino acid sequence of SEQ ID No. 20 or a fragment, analogue or derivative thereof.
The biological response may be one or more of MAP kinase activation, transcription factor activation and gene induction. The biological response may be inhibited by the presence ofa peptide comprising the amino acid sequence of SEQ ID No. 20 or a fragment, analogue or derivative thereof.
The biological sample may be cultured cells. Alternatively, the biological sample may be a nonhuinan animal.
The invention further provides for the use of a compound and/or substance identified by the method described herein as an adjuvant and/or booster of an immune response.
The invention also provides a vaccine comprising a compound and/or a substance identified by the method described herein.
The invention further provides a composition comprising a compound and/or a substance identified by the method described herein and a pharmaceutically acceptable excipient.
IE 0 9 0 8 7 5 - 18The invention also provides a peptide having between 4 to 27 amino acids comprising the amino acid sequence of SEQ ID No. 54, SEQ ID No. 55 or SEQ ID No. 4.
Pharmaceutical Compositions The invention extends in various aspects not only to a substance identified as a peptide, in accordance with what is disclosed herein, but also a pharmaceutical composition, medicament, drug or other composition comprising such a substance, a method comprising administration of such a composition to a patient, e.g. for treatment (which may include preventative treatment) to suppress an immune response mediated by TLR4, and a method of making a pharmaceutical composition comprising admixing such a substance with a pharmaceutically acceptable excipient, vehicle or carrier, and optionally other ingredients known to those skilled in the art. Such ingredients should be non-toxic and should not interfere with the efficacy ofthe active ingredient. The precise nature of the carrier or other material will depend on the route of administration, which may be, for example, oral, intravenous, intranasal or via oral or nasal inhalation.
I he formulation may be a liquid, for example, a physiologic salt solution containing nonphosphate buffer at pH 6.8-7.6, or a lyophilised or freeze dried powder.
Mimetics Non-peptide small molecules’ are often preferred for many in-vivo pharmaceutical uses. Accordingly, a mimetic or mimic of the substance may be designed for pharmaceutical uses.
I he designing of mimetics to a known pharmaceutically active compound is a known approach to the development of pharmaceuticals based on a lead” compound. This might be desirable where the active compound is difficult or expensive to synthesise or where il is unsuitable for a particular method of administration, e.g. peptides are not well suited as active agents tor oral compositions as they tend to be quickly degraded by proteases in the alimentary canal. Mimetic design, synthesis and testing may be used to avoid randomly screening large number of molecules for a target property.
Peptidomimetics Whilst numerous strategies to improve the pharmaceutical properties of peptides found to exert biological effects are known in the art including, forexample, amide bond replacements, incorporation of non-peptide moieties, peptide small molecule conjugates or backbone IE 0 9 Ο 8 Ί 5 - 19cyclisation. (lie optimisation of pharmacological properties for particular peptides still presents those involved in the optimisation of such pharmaceutical agents with considerable challenges.
Peptides of and for use in the invention may be modified such that they comprise am ide bond replacement, incorporation of non peptide moieties, or backbone cyclisation. Suitably if cysteine is present the thiol of this residue is capped to prevent damage of the free sulphate group.
Suitably a peptide of and for use in the invention may be modified from the natural sequence to protect the peptides from protease attack. Suitably a peptide of and for use in the invention may be further modified using at least one of C and / or N-terminal capping, and / or cysteine residue capping. Suitably a peptide of and for use in the invention may be capped at the N terminal residue with an aeetyl group. Suitably a peptide of and for use in the invention may be capped at the C terminal with an amide group. Suitably the thiol groups of cysteines are capped with acetamido methyl groups.
Non-peptide small molecules” are often preferred for many in vivo pharmaceutical uses. Accordingly, a peptidomimetic or mimic of the substance (particularly a peptide) may be designed for pharmaceutical uses. The designing of peptidomimetics to a known pharmaceutically active peptide is a known approach to the development of pharmaceuticals based on a lead” compound. This might be desirable where the active compound is difficult or expensive to synthesise of where it is unsuitable for a particular method of administration, e.g. peptides are not well suited as active agents for oral compositions as they tend to quickly degraded by proteases in the alimentary canal. Peptidomimetic design, synthesis and testing may be used to avoid randomly screening large number of molecules for a target property.
As used herein, a mimetic or peptidomimetic is a compound that is capable of mimicking a peptide. Peptidomimetics are generally not substrates of proteases and are likely to be active in vivo for a longer period of time as compared to the peptide on which they are based. In addition, peptidomimetics may be less antigenic and show an overall higher bioavailability.
There are several steps commonly taken in the design of a peptidomimetic from a compound having a given target property. Firstly, the particular parts of the compound that are critical and/or important in determining the target property are determined. In the case of a peptide, this ean be done by systematically varying the amino acid residues in the peptide, e.g. by substituting IE 0 9 0 8 7 5 -20each residue in turn. These parts or residues constituting the active region of the compound arc known as its “pharmacophore.
Once the pharmacophore has been found, its structure is modelled to according its physical properties, e.g. stereochemistry, bonding, size and/or charge, using data from a range of sources, e g. spectroscopic techniques, X-ray diffraction data and NMR. Computational analysis, similarity mapping (which models the charge and/or volume of a pharmacophore, rather than the bonding between atoms) and other techniques can be used in this modelling process.
In a variant of this approach, the three-dimensional structure of the ligand and its binding partner are modelled. This can be especially useful where the ligand and/or binding partner change conformation on binding, allowing the model to take account of this the design of the mimetic.
A template molecule is then selected onto which chemical groups which mimic the pharmacophore can be grafted. The template molecule and the chemical groups grafted on to it can conveniently be selected so that the mimetic is easy to synthesise, is likely to be pharmacologically acceptable, and does not degrade in vivo, while retaining the biological activity ofthe led compound. The peptidomimetic or peptidomimetics found by this approach can then be screened to see whether they have the target property, or to what extent they exhibit it. Further optimisation or modification can then be carried out to arrive at one or more final peptidomimetic for in vivo or clinical testing.
Fragments l he term f ragment1' used herein means two or more consecutive amino acid residues of a peptide sequence from which the fragment is derived. A fragment retains the biological activity of the peptide from which it is derived. For example SEQ ID No. 68 and SEQ ID No. 62 can be considered as fragments of SEQ ID No. 4 and SEQ ID No. 20.
Analogues and derivatives The invention extends to peptides which are derivales or homologues of A464 (SEQ ID No. 20). Thus, an analogue, homologue or derivative of any one of the peptides of the invention may include l. 2. 3. 4. 5 or greater than 5 amino acid alterations. te090875 -21 As is well understood, homology at the amino acid level is generally in terms of amino acid similarity or identity. Similarity allows for conservative variation’, such as substitution of one hydrophobic residue such as isoleucine, valine, leucine or methionine for another, or the substitution of one polar residue for another, such as lysine, glutamic acid for aspartic acid, or glutamine for asparagine.
Analogues of. and for use in, the invention as defined herein means a peptide modified by varying the amino acid sequence. Such derivatives of the amino acid sequence may involve insertion, addition, deletion and/or substitution for example conservative substitution of one or more amino acids.
Treatment / Therapy The term treatment* is used herein to refer to any regime that can benefit a human or non-human animal. 1 he treatment may be in respect of an existing condition or may be prophylactic (preventative treatment). Treatment may include curative, alleviation or prophylactic effects.
More specifically, reference herein to therapeutic and prophylactic treatment is to be considered in its broadest context. The term therapeutic does not necessarily imply that a subject is treated until total recovery. Similarly, prophylactic does not necessarily mean that the subject will not eventually contract a disease condition.
Accordingly, therapeutic and prophylactic treatment includes amelioration of the symptoms of a particular condition or preventing or otherwise reducing the risk of developing a particular condition, fhe term prophylactic may be considered as reducing the severity or the onset of a particular condition. Therapeutic may also reduce the severity of an existing condition.
Administration Peptides or derivatives thereof for use in the invention may be administered alone but will preferably be administered as a pharmaceutical composition, which will generally comprise a suitable pharmaceutical excipient, diluent or carrier selected depending on the intended route of administration.
IS 0 9 0 8 7 5 -22 Peptides or derivatives thereof for use in the invention may be administered to a patient in need of treatment via any suitable route. The precise dose will depend upon a number of factors, including the precise nature of the form of peptide to be administered.
Route ol administration may include; parenterally {including subcutaneous, intramuscular, intravenous, by means of, for example a drip patch), some further suitable routes of administration include (but are not limited to) oral, rectal, nasal, topical {including buccal and sublingual), infusion, vaginal, intradermal, intraperitoneally, intracranially, intrathecal and epidural administration or administration via oral or nasal inhalation, by means of, for example a nebuliser or inhaler, or by an implant.
In preferred embodiments, the composition is deliverable as an injectable composition, is administered orally, or is administered to the lungs as an aerosol via oral or nasal inhalation.
Por administration via the oral or nasal inhalation routes, preferably the active ingredient will be in a suitable pharmaceutical formulation and may be delivered using a mechanical form including, hut not restricted to an inhaler or nebuliser device.
For intravenous injection, the active ingredient will be in the form of a parenterally acceptable aqueous solution which is pyrogen-free and has suitable pH, isotonicity and stability. Those of relevant skill in the art are well able to prepare suitable solutions using, for example, isotonic vehicles such as sodium chloride injection, Ringer’s injection, Lactated Ringer’s injection. Preservatives, stabilisers, buffers, antioxidants and/or other additives may be included, as required.
Pharmaceutical compositions for oral administration may be in tablet, capsule, powder or liquid form A tablet may comprise a solid carrier such as gelatin or an adjuvant. Liquid pharmaceutical compositions generally comprise a liquid carrier such as water, petroleum, animal or vegetable oils, mineral oil or synthetic oil. Physiological saline solution, dextrose or other saccharide solution or glycols such as ethylene glycol, propylene glycol or polyethylene glycol may be included. -231 he composition may also be administered via microspheres, liposomes, other microparticulate delivery systems or sustained release formulations placed in certain tissues including blood. Suitable examples of sustained release carriers include semi permeable polymer matrices in the form of shared articles, e.g. suppositories or microcapsules. Implantable or microcapsular sustained release matrices include polylactides (US Patent No. 3, 773,919; EP-A-0058481) copolymers of 1 .-glutamic acid and gamma ethyl-L-glutamate (Sidman et al. Biopolymers 22( 1); 547-556. 1985). poly (2-hydroxyethyl-methacrylate) or ethylene vinyl acetate (Langer et al. J. Bionied. Mater. Res. 15: 167-277,1981, and Langer, Chem. Tech. 12:98-105, 1982).
Examples of the techniques and protocols mentioned above and other techniques and protocols which may be used in accordance with the invention can be found in Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems, 8lh Edition ISBN 0-781746-12-4, pages 186-505 and 653-671. the entire disclosures of which is herein incorporated by reference.
Dose Peptides or derivatives thereof according to the invention is preferably administered to an individual in a therapeutically effective amount’', this being sufficient to show benefit to the individual.
I he actual amount administered, and rate and time-course of administration, will depend on the nature and severity of what is being treated. Prescription of treatment, e.g. decisions on dosage etc. is ultimately within the responsibility and at the discretion of general practitioners and other medical doctors, and typically takes account ofthe disorder to be treated, the condition ofthe individual patient, the site of delivery, the method of administration and other factors known to practitioners.
I he optimal dose can be determined by physicians based on a number of parameters including, for example, age, sex, weight, severity of the condition being treated, the active ingredient being administered and the route of administration. feο 9 0 8 75 -24A subject in tlie context of the invention includes and encompasses mammals such as humans, primates and livestock animals (e. g. sheep, pigs, cattle, horses, donkeys); laboratory test animals such as mice, rabbits, rats and guinea pigs; and companion animals such as dogs and cats. It is preferred for the purposes of the invention that the mammal is a human.
Preferred features and embodiments of each aspect of the invention are as for each of the other aspects mutaiis mutandis unless the context demands otherwise.
Unless otherwise defined, all technical and scientific terms used herein have the meaning commonly understood by a person who is skilled in the art in the field of the invention.
Throughout the specification, unless the context demands otherwise, the terms comprise’ or 'include', or variations such as ‘comprises’ or comprising’, ‘includes’ or 'including' will be understood to imply the inclusion of a stated integer or group of integers, but not the exclusion of any other integer or group of integers.
The term “consists essentially of’ or “consisting essentially of’ as used herein means that a polypeptide may have additional features or elements beyond those described provided that such additional features or elements do not materially affect the ability of the polypeptide to function as a specific inhibitor ofTLR4 responses. That is, the polypeptide may have additional features or elements that do not interfere with specific inhibition of TLR4 responses. For example, a polypeptide consisting essentially of a specified sequence may contain one, two, three, four, five or more additional amino acids, at either end or at both ends of the sequence provided that the additional amino acids do not interfere with, inhibit, block or interrupt the specific inhibition of TLR4 responses. Similarly, a polypeptide molecule may be chemically modified with one or more functional groups provided that such chemical groups do not interfere with, inhibit, block or interrupt the specific inhibition of TLR4 responses.
Brief Description of the Drawings The invention will be more clearly understood from the following description of an embodiment thereof, given by way of example only, with reference to the accompanying drawings, in which:l-’ig. IΛ and B are bar charts showing the effect of peptides derived from A46 on C'pGinduced MIP-2 production murine RAW264.7 cells. Cells were seeded in 96 well plates # 0 9 08 7 5 -25(1.5x 10? cells/ml) at least 24 hours before treatment. A46 peptides were added to the cells at a concentration of 5 or 20 μΜ I hour before stimulating with lpg/ml C'pG. Supernatants were collected 24 hours after stimulation and assayed for M1P2 by ELISA. No peptides were added into the control wells (black bars). (A) peptides A461-A467 (SEQ ID NO. 17 to 23). (B) peptides A468-A4614 (SEQ ID NO. 24 to 30) (excluding A46I2 SEQ ID NO. 28). Data is representative of at least two experiments each performed in triplicate and is expressed as mean ± s.d; l;ig. 2A and B are bar charts showing the effect of peptides derived from A46 on CpGinduced IN Fa in murine RAW264.7 cells. A46 peptides were added to (he cells at a concentration of 5 or 20 μΜ 1 hour before stimulating with 1 pg/ml CpG. Supernatants were collected 24 hours after stimulation and assayed for TNFa by ELISA. No peptides were added into the control wells (black bars). (A) peptides A461-A467 (SEQ ID NO. 17 to 23). (B) peptides A468-A4614 (SEQ ID NO. 24 to 30) (excluding A4612 SEQ ID NO. 28). Data is representative of at least two experiments each performed in triplicate and is expressed as mean ± s.d; l’ig. 3 A and B are bar charts showing the effect of peptides derived from A46 on LPSinduced TNFa in human U937 cells. Cells were differentiated with 200nM PM A and seeded in 96 well plates (2 xlO5 cells/ml) 24 hours before treatment. A46 peptides were added to the cells at a concentration of 5 or 20 μΜ 1 hour before stimulating with 100 ng/ml LPS. Supernatants were collected 24 hours after stimulation and assayed for T NFa by ELISA. No peptides were added into the control wells (black bars). (A) peptides Λ461-Α467 (SEQ ID NO. 17 to 23). (B) peptides A468-A4614 (SEQ ID NO. 24 to 30) (excluding A4612 SEQ ID NO. 28). Data is representative of at least two experiments eaeh performed in triplicate and is expressed as mean ± s.d; I ig. 4 is a barchart showing the comparison of effect of Pl 3, A467 and A464 peptides on LPS induced TNFa in human THP-1 cells. Cells were seeded in 96 well plates (3x10^ cells/ml) and differentiated with 200nM PMA for at least 24 hours before treatment. P13 (SEQ ID No. 61), A467 (SEQ ID NO. 23) and A464 (SEQ ID NO. 20) peptides were added to the cells at a concentration of 20 or 40 μΜ 1 hour before stimulating with l()0ng/ml LPS. Supernatants were collected 6 hours after stimulation with the agonist and fc0 9 0 8 7 5 -26assayed for TNFa by ELISA. The peptides were added to the unstimulated wells at 40 μΜ. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 5A and B are bar charts showing the dose-dependent inhibitory effect of A464R on I PS-induced TNFot in murine RAW264.7 cells. A464 (SEQ ID NO. 20) was added to the cells at a concentration of 5, 20 or 50 μΜ 1 hour before stimulating with lOOng/ml LPS. Supernatant was collected 24 hours after stimulation and assayed for TNFa by ELISA (Λ) and cells were assayed for viability by the MTT assay (B.). No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; I ig. 6Λ and B are bar charts showing the effect of peptides derived from A46 on 1. PSinduccd TNFa in murine RAW264.7 cells. A46 peptides were added to the cells at a concentration of 1 or 5 μΜ 1 hour before stimulating with 10 ng/ml LPS. Supernatants were collected 6 hours after stimulation and assayed for TNFa by ELISA. No peptides were added into the control wells (black bars). (A) peptides A461-A467 (SEQ ID NO. 17 to 23). (B) peptides A468-A4614 (SEQ ID NO. 24 to 30) (excluding A4610 SEQ ID NO. 26 and Λ4612 SEQ ID NO. 28). Data is representative of at least two experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 7A and B are bar charts showing the effect of A464 on TNFa production and cell viability of human THP-1 cells stimulated with LPS. Cells were seeded in 96 well plates (3x 1 (f eells/ml) and differentiated with 200nM PMA for 24 hours before treatment. A464 (SEQ ID NO. 20) was added to the cells at a concentration of 5 or 10 μΜ 1 hour before stimulating with lOng/ml LPS. Supernatants were collected 6 hours after stimulation and assayed for TNFa by ELISA (A) and cells were assayed for viability by the MTT assay (B), No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 8 is a bar chart showing an investigation of the inhibitory potential of A464 when added to cells after, during and before stimulation with LPS. THP-I cells were seeded in 96 well plates (3xl05 cells/ml) and differentiated with 200nM PMA for 24 hours before tE 0 9 0 8 7 5 -27ireatment. A464 (SEQ ID NO. 20) was added to the cells at a concentration of 5 or 10 μΜ. Cells were stimulated at different time points in relation to addition ofthe peptide: I hour before adding the peptide (-60’), at the same time as peptide (O’), or 1 hour after the peptide was added (+60’). Supernatants were collected 6 hours after stimulation and assayed for TNFa by ELISA. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 9A and B are bar charts showing the effect of A464 on Pam3CysK4-induced TNFa in human THP-1 cells. Cells were seeded in 96 well plates (3xlO5 cells/ml) and differentiated with 200nM PMA for 24 hours before treatment. A464 (SEQ ID NO. 20) was added to the cells at a concentration of 1, 5 or 10 μΜ 1 hour before stimulating with either lOOng/ml (A) or lOng/ml Pam3CysK4 (B). Supernatants were collected 24 hours after stimulation and assayed for TNFa by ELISA. Cells from (B) were also assayed for viability by the MTT assay (C). No peptides were added to the control wells (black bars) Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig, 10A and B are bar charts showing the effect of A464 on po!y(I:C)-induced TNFa in murine RAW264.7 cells. Cells were seeded in 96 well plates (1.5xl05 cells/ml) 24 hours before treatment. A464 (SEQ ID NO. 20) was added to the cells at a concentration of 5. 20 or 50 μΜ 1 hour before stimulating with 25pg/ml Poly(LC). Supernatants were collected 24 hours after stimulation and assayed for TNFa by ELISA (A) and cells were assayed for viability by the MTT assay (B). No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 11A and B are bar charts showing the effect of A464 and scrambled A464 (ConA464) on poly(I;C)-induced TLR3 signalling in HEK293 cells. HEK293 TLR3 cells were seeded in 96 well plates (1.5xl05 cells/ml) 24 hours before transfection with the plasmid DNA. Cells were transfected with 60ng of either ISRE-Luciferase (A) or NFTB-Luciferase (B) and 20ng of TK-Renilla reporter gene plasmids. The total volume of plasmid DNA transfected into the cells was made up to 230ng by adding pcDNA. /£ 0 9 0 8 7 5 -28Scrambled A464 (ConA464) (SEQ ID NO. 32) and A464 (SEQ ID NO. 20) peptides were added to the cells at a concentration of 2, 5 or 10 μΜ the next day and cells were stimulated with 25pg/ml poly(I:C) 1 hour after treatment with the peptide. Cells were harvested and reporter gene assays performed 6hr after stimulation with the agonist. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate. Data are expressed as mean fold induction ± s.d. relative to control levels; f ig. 12 is a bar chart showing the effect of A467 and A464 on TLR8-induced NFkB activation in HEK293 cells. HEK293_TLR8 cells were transfected with 60ng NFkBLuciferasc and 20ng of TK-Renilla reporter genes, as in Fig. 11. A467 (SEQ ID NO. 23) and A464 (SEQ ID NO. 20) were added to the cells at a concentration of 1. 5 or 25 μΜ the next day and cells were stimulated with 2.5 μ$0η1 CL075 1 hour after treatment with the peptide. Cells were harvested and assayed for NFkB activation by reporter gene assay 6hr after stimulation with the agonist. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate. Data are expressed as mean fold induction ± s.d. relative to control levels; Fig. 13A and B are bar charts showing the effect of A467 and A464 on LPS-induced I N Fa production and on cell viability in primary human cells. Peripheral blood mononuclear cells (PBMCs) were seeded in 96 well plates (1 xIO6 cells/ml) 24 hours before treatment. A467 (SEQ ID NO. 23) and A464 (SEQ ID NO. 20) were added to the cells at 1. 5 and 25 μΜ 1 hour before stimulating with 10 ng/ml LPS. The peptides were added into the unstimulated control wells at 25 μΜ. Supernatants were collected 6 hours after stimulation and assayed for TNFa production by ELISA (A). The cells were assayed for viability by the MTT assay (B). No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 14A and B are bar charts showing the comparison of the inhibitory effect of the Land D- forms of A464 on LPS-induced TNFa in primary human and murine cells. PBMC (1 xl06 cells/ml, (A)) or immortalised murine macrophages (1.5xlO5 cells/ml. (B)) were seeded in 96 well plates 24 hours before treatment. L-form and D-form A464 peptides -29IE 0 9 08 7 5 (with the 9R delivery peptide on the C-terminus, SEQ ID NO. 20) were added to the cells at a concentration of 1, 5 or 25 μΜ 1 hour before stimulating with 10 ng/ml LPS. Supernatants were collected 6 hours after stimulation and assayed for either human (A) or murine (B) TNFa production by ELISA. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. I5A and B are bar charts showing the effect of the D-form A464 peptide with the ΤΛΤ delivery sequence on LPS induced TNFa in primary human and murine cells. PBMC (A) and immortalised murine macrophages (B) were seeded in 96 well plates 24 hours before treatment. D-form of A467 (SEQ ID NO. 37) and A464 (SEQ ID NO. 36) with the TAT delivery sequence were added to the cells at a concentration of I, 5 or 25 μΜ 1 hour before stimulating with 10 ng/ml LPS. The peptides were added into the unstimulated control wells at 25 μΜ. Supernatants were collected 6 hours after stimulation and assayed for either human (A) or murine (B) TNFa production by ELISA. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 16A and B are bar charts showing the effect of attaching 9R to the N-terminus of A464 on LPS-induced TNFa in primary human and murine cells. Immortalised murine macrophages (A) and PBMC (B) were seeded in 96 well plates 24 hours before treatment. L-fbrm of A467 (SEQ ID NO. 39) and A464 (SEQ ID NO. 38) with the 9R-delivery sequence at the N-teminus were added to the cells at a concentration of I. 5 or 25 μΜ 1 hour before stimulating with 10 ng/ml LPS. The peptides were added into the unstimulated control wells at 25 μΜ. Supernatants were collected 6 hours after stimulation and assayed for either murine (A) or human (B) TNFa production hy ELISA. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. 17A and B are bar charts showing the effect of the D-form A464 with 9R at the Ntermintis on LPS-induced TNFa in primary human and murine cells. Immortalised murine macrophages (A) and PBMC (B) were seeded in 96 well plates 24 hours before treatment. D-form of A467 (SEQ ID NO. 39) and A464 (SEQ ID NO. 38) peptides with IE Ο 9 Ο 8 Ί 5 -30the 9R-delivery sequence at N-teminus were added to the cells at a concentration of k 5 or 25 μΜ 1 hour before stimulating with 10 ng/ml LPS. The peptides were added into the unstimulated control wells at 25 μΜ. Supernatants were collected 6 hours after stimulation and assayed for either murine (A) or human (B) TNFa production by ELISA.
No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; Fig. ISA and B are bar charts showing the effect of shortened A464 peptides with deletions of N- and C-terminal amino acids on LPS-induced TNFa in murine macrophages. Immortalised murine macrophages were seeded in 96 well plates (I.5xl0? cells/ml) 24 hours before treatment. A464 peptides with a deletion of one (N-1) (SEQ ID NO. 40) or two (N-2) (SEQ ID NO. 41) amino acids from the N-terminus (A), or three (C-3) (SEQ ID NO. 42) or six (C-6) (SEQ ID NO. 43) amino acids from the C-terminus (B). were added to the cells at a concentration of 1, 5 or 25 μΜ 1 hour before stimulating with 1 Ong/ml LPS. Peptides were added to the control wells at 25 μΜ. Normal full-length A464 (SEQ ID NO. 20) was used at 5 μΜ as a positive control. Supernatants were collected 6 hours after stimulation and assayed for TNFa by ELISA. No peptides were added lo the control wells (black bars). Data is representative of at least three experiments eaeh performed in triplicate and is expressed as mean ± s.d; Fig. I9A and B are bar charts showing the effect of shortened A464 peptides with deletions of N- and C-terminal amino acids on LPS-induced TNFa in human PBMC. A464 peptides with a deletion of one (N-l) (SEQ ID NO. 40) or two (N-2) (SEQ ID NO. 411 amino acids from the N-terminus (A), or three (C-3) (SEQ ID NO. 42) or six (C-6) (SEQ ID NO. 43) amino acids from the C-terminus (B), were added to the cells at a concentration of I, 5 or 25 μΜ I hour before stimulating with 1 Ong/ml LPS. Peptides were added to the control wells al 25 μΜ. Normal full-length A464 (SEQ ID NO. 20) was used at 5 μΜ as a positive control. Supernatants were collected 6 hours after stimulation and assayed for TNFa by ELISA. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d: -31 IB 0 9 0 8 7 5 l ig. 20Λ and B are bar charts showing the effect of shortened A464 peptides with deletions of N- and C-terminal amino acids on TLR4- and TLR3-induced NFkB activation in HEK293 cells. HEK293 TLR4 (A) and HEK293 TLR3 (B) cells were seeded in 96 well plates (1.5x10^ cells/ml) 24 hours before transfection with the plasmid DNA. Cells were transfected with 60ng NFicB-Luciferase and 20ng of TK-Renilla reporter genes, as in Fig. 11. A464N-2 (SEQ ID NO. 41) and A464C-3 (SEQ ID NO. 42) peptides were added to the cells at a concentration of 1,5 or 25 μΜ the next day and cells were stimulated with either lOng/ml LPS (A) or 25pg/ml Poly(LC) (Β) 1 hour after treatment with peptides. Cells were harvested and assayed for NEkB activation by reporter gene assay 6 hours after stimulation with the agonists. No peptides were added to the control wells (black bars). Data is representative of three experiments eaeh performed in triplicate and is expressed as mean fold induction ± s.d. relative to control levels: l ig. 21A and B are bar charts showing the results of an alanine scan of A464 in human PBMC and murine macrophages. Primary human PBMC (1 xlO6 cells/ml. (A)) and immortalised murine macrophages (l.SxlO5 cells/ml, (B)) were seeded in 96 well plates 24 hours before treatment. Peptides with an Alanine substitution at each position in A464 (SEQ ID NO. 44 to 53) were added lo the cells at a concentration 5 μΜ 1 hour before stimulating with lOng/ml LPS. Supernatants were collected 6 hours after stimulation with the agonist and assayed for human (A) and murine (B) TNFa by ELISA. Normal A464 (SEQ ID NO. 20) peptide was used at 5 μΜ as a positive control. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; f ig. 22 Λ and B are bar charts showing the effect of short A467 and A464 peptides, with 9k on either N- orC-terminus, on LPS- induced TNFa production in murine RAW264.7 cells. Cells were seeded in 96 well plates (l.5xl05 cells/ml) 24 hours before treatment. Short A467 and short A464 peptides with the 9R delivery sequence on either the Ctei minus (sA464C9R) (SEQ ID NO. 56) (A) or the N-terminus (SA464N9R) (SEQ ID NO. 57) (B) were added to the cells at a concentration of 5 or 25 μΜ 1 hour before stimulating with lOng/ml LPS. Normal full length A467 (SEQ ID NO. 23) and A464 (SEQ ID NO. 20) peptides were added at a concentration of 5 μΜ as positive controls. Supernatants were collected 6 hours after stimulation with the agonist and assayed for IE 0 9 0 8 7 5 -32TNFa by ELISA. No peptides were added to the control wells (black bars). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d; F ig. 23Λ is a bar chart showing the effect of shorter versions of SA464N9R (SEQ ID NO. 57) peptide on TLR4-induced murine TNFa in immortalised murine macrophages. BMDM cells were seeded in 96 well plates (1.5xl05 cells/ml) 24 hours before treatment. Short peptide sA464N9R (SEQ ID NO. 57) with a deletion of one amino acid from either N- terminus (9R-FKLIL) (SEQ ID NO. 62) or C-terminus (9R-SFKL1) (SEQ ID NO. 63) or both (9R-FKLI) (SEQ ID NO. 64) and one with substitution of Isoleucine (1) to Phenylalanine (F) (9R-SFKLFL) (SEQ ID NO. 65), were added to the cells at 1. 3. 6.25 and 12.5 μΜ 1 hour before stimulating with 20ng/ml LPS. Peptides were added to the control wells at 50 μΜ. Short sA464N9R (SEQ ID NO. 57) was used at 12.5 μΜ as positive control and SA467N9R was used as negative control. Supernatants were collected 6 hours after stimulation and assayed for TNFa by ELISA. No peptides were added to the control wells (black bars). The cells were also assayed for viability hy the MTf assay (Fig. 23B). Data is representative of at least three experiments each performed in triplicate and is expressed as mean ± s.d.
Pig. 24A and B are bar charts showing the effect of A464 on ligand-independent TLR4 signalling in HEK293 cells. HEK293 TLR4 cells were seeded in 96 well plate (1.5x1(T cells/ml) 24 hours before transfection with NFicB-Iuciferase and TK-Rcnilla reporter gene plasmids. NFkB activation was stimulated by transfecting either 50 or lOOng of ('1)4/1 LR4-encoding plasmid or by stimulating with lOng/ml LPS. The total amount of plasmid DNA transfected into the cells was made up to 2 3 Ong by adding pc DNA. A 464 (SEQ ID NO. 20) was added to the cells at a concentration of 5 or 25 μΜ 2 hours (A) or 9 hours (B) after the transfection or LPS-stimulation. Cells were harvested and assayed for NFkB activation by reporter gene assay 24 hours post-transfection. No peptides were added to the control wells (black bars). Data is representative of two experiments, each performed in triplicate and is expressed as mean fold induction ± s.d. relative to control levels; Fig. 25A and B are immunoblots showing the effect of A464 on LPS-induced ΙκΒα degradation in murine RAW264.7 cells. Cells were seeded in 6-well plates at 1.5x1 (f IB 0 9 0 8 7 5 -33cells/ml 24 hours prior to treatment with peptides. A467 (SEQ ID NO. 23) and A464 (SEQ ID NO. 20) were then added to the wells 1 hour before stimulation at a concentration of 5 μΜ. Cells were stimulated with lOng/ml LPS for a period of 5. 15. 30 or 60 min and then harvested on ice and lysed in 1% NP-40 containing lysis buffer. Lysates then were denatured using 5x sample buffer with DTT and resolved by SDSPAGE. Ihe proteins were transferred to PVDF membrane and the membrane was iminunoblotted for ΙκΒα (A). Equal protein loading was confirmed by re-probing the blot for β-actin (B). The blots shown are representative of two experiments; Pig. 26 is a bar chart showing the effect of A464 on LPS-induced IRF3 activation in HEK293 cells. HEK293_TLR4 cells were seeded in 96 well plate (1.5x105 cells/ml) 24 hours before transfection with the plasmid DNA, Cells were transfected with a plasmid encoding IRF3-GAL4, together with pFR-luciferase and TK-Renilla reporter gene plasmids. The total amount of plasmid DNA transfected into the cells was made up to 236 ng/well by adding pcDNA. A464 (SEQ ID NO. 20) was added to the cells at a concentration of 1,5 or 10 μΜ the next day, and cells were stimulated wilh lOng/ml LPS 1 hour after treatment with the peptide for 6 hours. Cells were harvested and assayed lor IRI 3 activation by reporter gene assay. No peptides were added to the control wells (black bars). Data is representative of two experiments, each performed in triplicate and is expressed as mean fold induction ± s.d. relative to control levels; I ig 27A, B and C are immunoblots showing the effect of A464 on LPS-induced appearance of phospho-JNK in murine RAW264.7 cells. Cells were set up as for Fig. 25 above and 5 μΜ A467 (SEQ ID NO. 23) or A464 (SEQ ID NO. 20) were added to the wells I hour before stimulation. Cells were stimulated with lOng/ml LPS for a period of . 15. 30 or 60 min, then harvested and lysed. Proteins were resolved by SDS-PAGE. and transferred membranes immunoblotted for phospho-JNK. (A), total JNK (B) or β-actin (C). I he blots shown are representative of two experiments; l ig. 28Λ and B are immunoblots showing the effect of A464 on LPS-induced appearance of pho$pho-p38 MAPK in murine RAW264.7. Cells were set up as for Fig. 24 above and 5 μΜ Λ467 (SEQ ID NO. 23) or A464 (SEQ ID NO. 20) were added to the wells 1 hour before stimulation. Cells were stimulated with lOng/ml LPS for a period of 5, 30 or 60 -34te 0 9 0 g 7 5 milk then harvested and lysed. Proteins were resolved by SDS-PAGE. and transferred membranes immunoblotted for phospho-p38 (A) or total p38 (B). The blots shown are representative of two experiments; Fig. 29 shows an immunoblot of a His-pulldown of over-expressed TLR4 adaptor proteins Mai and TRAM with His-tagged A464 peptide by affinity chromatography. IILK293 T cells were seeded in 6 well plate (1.5xl05 cells/ml) 24 hours before transfection with 1 pg/well Flag-Mal and 2 pg/well Flag-TRAM encoding plasmids. The total amount of plasmid DNA transfected into the cells was made up to 2 pg by adding pcDNA. 24 hours post-transfection cells were lysed in 1% NP-40 lysis buffer containing 20 mM imidazole. The experimental lysates were incubated with 25 μΜ His-A464 peptide ((11-H-H-H-H-H-KYSFKLILAEY) (SEQ ID NO. 66) and 40 pL of Ni-agarose beads for 2 hr at 4°C. Control samples were incubated with either heads only or beads and 25 μΜ His-A467 peptide (H-H-H-H-H-H-RNT1SGNIYSA) (SEQ ID NO. 67). After incubation beads were washed 5 times in lysis buffer with imidazole. Proteins were resolved by SDS-PAGE, and transferred membranes immunoblotted for Flag. Data is representative of four experiments; and Fig. 30 is a graph showing that A464 inhibits LPS-induced IL-12/23 p40 in vivo. Groups of 5 female BALB/c mice were injected i.v, with a single bolus of one of the following; PBS, 1 pg LPS, 1 pg LPS with 3mg/kg D-9RN-A464 (SEQ ID NO. 38), 1 pg LPS with 3nig/kg L,-9RN-A464 (SEQ ID NO. 38), or 1 pg LPS with 3mg/kg L-9RN-A467 (SEQ ID NO. 39), Four hours later, blood was harvested and serum derived. The serum was assayed for !L-12p40 by ELISA. Concentrations are expressed as pg/ml. * p<0.05. *** p<0.()01 samples versus LPS. # p<0.01 samples versus PBS. Serum levels oflL-l2p40 in mice receiving D-9RN-A464 or L-9RN-A464 with LPS were not significantly di lie rent from mice receiving only PBS.
Detailed Description Vaccinia virus proteins A52R and A46R have a distinct profile of inhibition against the TLR system, it may be possible to generate a repertoire of peptides with differential anti-inflammatory effects on the TLR system, whereby some peptides would specifically inhibit one distinct TLR signalling axis, while other peptides would block a number of pathways. This is particularly IE 0 9 0 8 7 5 -35important to preserve anti-pathogen immunity while inhibiting an over active inflammatory response that causes pathogenesis. Although exogenous proteins with intracellular targets are unlikely to be attractive therapeutics, peptides based on critical amino acid sequences from inhibitory proteins, and peptide derivatives such as peptidomimeties have more potential. In fact others have shown that a peptide containing 11 amino acids of the A52R sequence fused to a cel l-penei rating peptide (called Pl3, SEQ ID NO. 61) can reduce in vivo bacterial-induced inflammation, and LPS-induced inflammatory mediators in mice (McCoy ei ul, 2005; Tsung et ah 2007).
We describe an 11 amino acid peptide derived from the A46 protein sequence that inhibits TLR4 responses when fused to a cell-penetrating peptide. This peptide has several potential advantages over P13 (SEQ ID NO. 61): firstly, it is specific for one TLR, namely TLR4. This is surprising given that full-length A46 protein can inhibit multiple TLRs. Secondly it is active in human cells, which is not the case for PI3 (SEQ ID NO. 61) (see Fig. 4). Thirdly, it inhibits TLR4 responses in vitro at a lower concentration than that reported for Pl 3 (SEQ ID NO. 61. McCoy et al. 2005): fig. 14 shows significant inhibition of TLR4 by A464 (SEQ ID No. 20) at 1 μΜ. in both murine and human cells. Furthermore, shorter forms of the peptide, namely SEK1.IL (SEQ ID NO. 55) and FKLIL (SEQ ID NO. 62) are also shown to inhibit TLR4 when fused to a delivery peptide, making the development of a peptidomimetic more likely than would be the case with Pl 3 (SEQ ID NO. 61).
Peptides derived from mammalian TIR protein BB loops have been shown to inhibit TLR signalling in vitro (Toshchakov et al, 2005; Loiarro et al, 2005; Toshchakov and Vogel, 2007; Toshchakov et al, 2007), which might suggest that a peptide from the A46 BB loop would also be inhibitory. However, the peptide discovered here, A464 (SEQ ID NO. 4 and 20), is from a region of A46 upstream of the BB loop. In fact, peptides derived from the A46 BB loop fused to cell-penetrating peptides, such as A467 (SEQ ID NO. 23), were found not to be inhibitory. Interestingly, A464 (SEQ ID NO. 20) has activity against TLR4 at a much lower concentration than peptides from mammalian BB loops (Toshchakov et al, 2007), and acts in a more specific manner since the only TLR it targets is TLR4.
The invention will be more clearly understood from the following examples.
Examples IE 0 9 0 8 7 5 -36Materials and Methods: Plasmids lhe empty vector pcDNA3.1 and the pFR-luciferase reporter construct containing five yeast Gal4 binding sites were purchased from Stratagene. NFkB-luciferase reporter construct containing 5x kB elements inserted into a pGL3 basic vector was obtained from R. Hofmeister, University ol Rosenburg (93042 Rosen burg, Germany). The TK-Renilla luciferase reporter plasmid containing the Herpes Simplex Virus (HSV) thymidine kinase promoter and the ReniUa reniformis luciferase gene in a pRL vector was from Promega. The plasmid encoding the fusion protein CD4-TLR4 was a gift from R. Medzhitov (Yale University, New Haven, CT. USA). The plasmid encoding TLR3 was a gift from D. Golenbock (University of Massachusetts Medical School. Worchester, MA, USA). The plasmids encoding Flag-Mal and Flag-TRAM were gifts from K. Fitzgerald (University of Massachusetts Medical School, Worchester, MA. USA).
Cell culture The human embryonic kidney cell line 293 (HEK293) and HEK293 cells stably transfected with the Interleukin-1 Receptor (HEK293 R1) were a gift from Tularik Inc (San Francisco, CA 94080. USA). 1IEK293 cells stably transfected with Toll-Like Receptor (TER) 2, 3, 4 and 8 (HEK293 TI.R2. HEK293_TLR3, HFK293 TLR4 and HEK293 TLR8) were a gift from K. Fitzgerald (University of Massachusetts Medical School, Worcester, MA, USA). Immortalised murine macrophages derived from bone marrow were a gift from D. Golenbock (University of Massachusetts Medical School, Worcester, MA, USA). The mouse leukaemia monocytemacrophage cell line RAW264.7, the human acute monocytic leukemia cell line TUP-1 and the human leukemic monocyte lymphoma cell line U937 were obtained from the European Collection of Animal Cell Cultures (ECACC, Salisbury, UK). Adherent cells were grown in Dulbecco's Modified Eagle's Medium (DMEM) and suspension cells were grown in RPMI medium supplemented with 10% (v/v) heat inactivated foetal calf serum (PCS). 2mM Lglutamine and IOOpg/ml gentamycin (referred to as complete medium). Cells were subcultured every two to three days when 60-80% confluent. Human peripheral blood mononuclear cells (PBMC) were isolated from the buffy coat of heparinized whole blood by density centrifugation on low-endotoxin ficoll-hypaque. Isolated PBMCs were washed three times in sterile phosphatebuffered saline (PBS: 137mM NaCI, 2.7mM KCI, lOmM Νθ2ΗΡΕ>4, 2mM NaHjPOq), counted and seeded al a density of lxl06cells/ml in complete RPMI medium.
Antibodies IE 0 9 0 8 7 5 -37Antibodies used were mouse anti-p-actin antibody (Sigma), anti-Flag monoclonal antibody (Sigma), rabbit polyclonal anti-phosphospecific p38 antibody (Cell Signalling), anti-p38 antibody (Cell Signalling), rabbit polyclonal anti-phosphospecific JNK antibody (Biosource). anli-JNK antibody (Biosource), and mouse monoclonal anti-ΙκΒα antibody (a gift from Ron Hay. University of Dundee, Scotland).
Receptor Agonists Ultra-pure lipopolysaeeharide (LPS) from Eschericia eoli (>99.9% pure in respect to protein. DNA and TLR2 agonists contaminants) was from Alexis Corporation, the synthetic doublestranded kN A poly(LC) was from Amersham Biosciences, IL-la was from the National Cancer Institute (Frederick, WA, USA) and TNFa was a gift from Zeneca Pharmaceutics (Macclesfield, UK). Cp(J DNA and PM A were a gift from K. Mills (Trinity College Dublin, Dublin, Ireland).
Reconstitution of peptides Peptides were chemically synthesised commercially by Genscript (www.genscript.com) and were at least 95% pure. Peptides were reconstituted with molecular biology grade sterile water to I OmM and stored at -80°C. Working stocks of ImM and 200μΜ were stored at 4°C for a maximum of 2 weeks or else kept at -20°C.
Enzyme-Linked ImmunoSorbent Assay (ELISA) Cytokine and chemokine production from cells or mouse serum was measured by LLISA using Duoset kits Irom R&D systems, according to the manufacturer’s instructions.
MTT assay Cell viability was measured by assessing mitochondrial function using an MTT (3-(4.5Dimcthylthiuzol-2-yl)-2,5-diphenyItetrazolium bromide) assay. Cells were seeded into 96 well plates and treated with peptides and agonists as described above in relation to the Figs. Wells containing medium only served as a blank control. To terminate treatment of cells, medium was removed and the cells were washed once with PBS. 200μ1 per well of Img/ml MTT in PBS was added directly lo the cells and the plates were incubated at 37°C for 14-16 hrs in the dark. MTT was then removed and 200μ1 per well of dimethyl sulphoxide (DMSO) was added and cells incubated for 20min at 37°C in the dark, prior to absorbance being read at 595nm on a spectrophotometer.
IE 0 9 0 8 7 5 -38Reporter gene assays I IEK293 cells were transfected using GeneJuice® a proprietary non-toxic transfection reagent. 200 μΐ/wclI cells at a density of lxl 05 cells/ml were seeded the day before transfection. 0.8 μ| of GeneJuice was mixed with 9.2 μΙ of serum-free DMEM (SFM) per transfection and incubated for 5 min at room temperature before adding the appropriate amount ofthe plasmid DNA. Ihe GeneJuice/ Sl’M/DNA mixture was then incubated at room temperature for 15 min before adding to the cells. 10μ1 per well of the GeneJuice/SMF/DNA mixture was added to the cells in triplicate and the cells were allowed to recover for 18-20 hours before stimulation. I or the NKkB and ISRE reporter gene assays, 60ng per well of either NFtcB-luciferase or ISRE-luciferase reporter genes were used, together with 20ng of the constitutively active internal control Renilfaluci(erase gene reporter. To measure IRF3 activation, the Stratagene PathDetect System™ was used which employs a plasmid encoding a fusion protein of IRF3 and the Gal4 DNA-binding domain (DBD), together with the pFR-luciferase reporter plasmid which contains five yeast Gal4 binding sites that control expression of the firefly luciferase gene. The DBD ofthe fusion transactivator protein binds to the reporter plasmid at the Gal4 binding sites. Phosphorylation of the IRF3 transcription activation domain ofthe fusion trans-activator protein activates transcription of the luciferase gene from the reporter plasmid. Therefore, activity of luciferase reflects the activation of IR13. As before, RenMla-Uxciferase gene reporter plasmid was used as an internal control. In all cases the total amount of DNA per well was kept constant at 230ng by the addition of pcDNA3.1. Cells were harvested 6 hours post-stimulation and lysed for 20 min using 50 pi of passive lysis buffer (Promega, Madison, Wisconsin, USA) with vigorous shaking. 20 μΐ of the lysates were placed in two different plates to analyse for Firefly and Rentfla luciferase activity.
I he substrate for firefly-luciferase was 40 μ| of luciferase assay mix (20mM tricine, I.O7mM (MgCOri-iMgtOHb 5H2O, 2.67mM MgSC>4, 0.1 M EDTA, 33.3mM DTT. 270mM coenzyme A. 470mM luciferin and 530mM ATP), and the substrate for Rentfla luciferase was 40 μ I of coelentrazinc (2pg/ml in PBS). Luciferase activity was analysed using a luminometer.
Western Blot analysis RAW264.7 cells were seeded at a density of lx105 cells/ml in 6 well plates. After treatment with peptides and agonists, as described above in relation to the Figs, the medium was removed and the cells were washed once with PBS before scraping them in 100μ1 lysis buffer (lOOmM NaCL 50mM 1ILPES (pH 7.5), ImM EDTA, 0.5% NP-40, 10% Glycerol) and incubating them on ice fe0 9Οβ 75 -39I'or 40 min. Samples were spun at 15,000 x g for lOmin and supernatants collected and stored at 20°C. Λ Bradford assay was used to determine the protein concentration in the samples. ΙμΙ of the sample was added to 480μ1 Quick Start® Bradford Dye Reagent (BioRad Laboratories Inc. Hercules. CA 94547, USA) together with I9pl deionised distilled water. The protein concentration was determined by monitoring the change in the solution absorbance at 280nm using a spectrophotometer compared to protein standards. Cell lysates with a known protein concentration were then diluted with water to obtain a concentration of 30-40 pg/ml protein in each sample. 20μΙ of the diluted samples were mixed with 5x Sample Buffer (62.5mM Tris pH 6.8. 2% (w/v) SDS, 10% (v/v) Glycerol, 0.1% (w/v) Bromophenol Blue, 50mM DTT) and boiled for 5 min at 99°C. Cooled samples were resolved on a sodium dodecyl sulphate (SDS) polyacn lam ide gel, the resolved proteins were transferred to Immobilon™ polyvinylidene di fluoride (PVDF) membrane, and membranes were blocked for I hour at room temperature or over night at 4°C. With 5% (w/v) Marvel™ (non-fat dried milk) in 1% (v/v) PBS-Tween for ΙκΒα. INK. β-actin and p38 and 1% (v/v) TBS-Tween for phospho-JNK and phospho-p38. The membrane then was incubated in the relevant primary antibody diluted 1:1000 in either 5% (w/v) Marvel™ in 1% (v/v) PBS-Tween for IkB. JNK, β-actin and p38 and in 3% (w/v) BSA in l%(v/v) TBS-Twcen for phospho-JNK and phospho-p38, for 2 hours at room temperature or overnight at 4°C. Thereafter, the membranes were washed, incubated in appropriate secondary antibody and then developed using standard enhanced chemi-luminescence (ECL) reagents.
His peptide pulldown assay HEK293 T cells were seeded in 6 well plates <1.5x105 cells/ml) 24 hours before transfection. 8 pi of GeneJuice was mixed with 32 μΙ of serum-free DMEM (SFM) per transfection and incubated for 5 min at room temperature before adding 1 pg per transfection of Flag-Mal or 2 pg of Flag-TRAM encoding plasmid DNA. GeneJuice/ SFM/DNA mixture was then incubated for 15 niin at room temperature before adding to the cells. The total amount of plasmid DNA transfected into the cells was made up to 2 pg by adding pcDNA. 40pl per well of the final mixture was added per welt. 24 hours post-translection supernatant was removed and cells were washed once with ice-cold lx PBS before lysing in 600 pi of 1% NP-40 lysis buffer (lOOmM NaCI. 50mM I1EPES (pH 7.5), ImM EDTA, 1% NP-40, 10% Glycerol) containing 20 mM imidazole on ice for 45 min. The lysates containing either Flag-Mal or Flag-TRAM were pooled together to ensure equal amount of the protein in each sample. 550 pi of lysates were incubated with 25 pM of either His-A464 (H-H-H-H-H-H-KYSFKLILAEY - SEQ ID No 66) or His# 0 9 08 75 -40Λ467 (ΙΙ-ΗΉ-ΙΙ-Η-Η-RNTISGNIYSA - SEQ ID No. 67) peptide and 40 pL of Ni-agarose beads or beads only (control) for 2 hr at 4°C rolling to avoid sedimentation of the beads. After incubation beads were washed 5 times in 1% NP-40 lysis buffer with 20 mM imidazole. After the final wash the buffer was completely removed and 35 μΙ of 1.5x Sample Buffer (62.5mM Tris pH 6.8. 2% (w/v) SDS, 10% (v/v) Glycerol, 0.1% (w/v) Bromophenol Blue, 50mM DTT) was added to each tube. Samples were boiled for 5 min at 99°C and then were resolved on 10% sodium dodecyl sulphate (SDS) polyacrylamide gel. The resolved proteins were transferred to Immobilon™ polyvinylidene difluoride (PVDF) membrane, and membranes were blocked for 1 hour at room temperature or over night at 4°C in blocking buffer (5% (w/v) Marvel™ (non-fat dried milk) in 1% PBS-Tween). The membrane then was incubated in the anti-Flag primary antibody diluted T. 10000 in the blocking buffer, for 2 hours at room temperature or overnight at 4°C. Thereafter, the membranes were washed 6 times in 1% (v/v) PBS-Tween, and then incubated in anti-mouse secondary antibody diluted 1:10000 in blocking buffer. The signal was read using Odyssey Infrared Imaging System, Ll-COR® Biosciences.
Example I - Identification of TLR inhibitory peptides derived from VACV A46 As the full-length A46 protein can inhibit TLR signalling and TLR-dependent gene induction (Stack el al. 2005). peptides derived from the A46 protein were assayed for TLR inhibitory effects. Secondary prediction programs suggest that A46 folds like a TIR domain, consistent with its ability to interact with mammalian TIR-containing proteins. A46-derived peptides were designed based on two regions of the A46 sequence that represent conserved TIR motifs. Firstly, the BB loop ofthe TIR domain is know to be important for signalling, and may mediate proteinprotein interactions. Further, peptides derived from mammalian TIR protein BB loops have been shown to inhibit TLR signalling (Toshchakov el al, 2005; Loiarro et al, 2005; Toshchakov and Vogel. 2007; Toshchakov et al, 2007). Secondly, another region thought to be important for TIR-FIR interactions is the CD/DD loop. Truncated versions of A46 which retained the predicted CD/DD loop region are still inhibitory against TLRs. Therefore this region was also considered of interest. The predicted position ofthese loops in A46 (from Western Reserve strain of VACV. Protein ID: P26672) is shown below (underlined): ΜΛΓΙ)137\Α8ΚΊ LNALVYFSTQQNKLVIRNEVNDTHYTVEFDRDKWDTFISYNRHNPTJEIRGVLPEF.TN 2 3 4 5 6 7 8 S IGG^VtlYPvSMTVLYNKYSFKLILAEYIRHRNTISGNlYSALMTLDDLAIKQYGDIDLLFNEKLKVPSPSG IE Ο 9 Ο 8 7 5 -41 BR J '; Ί 12 13 14 15 L ΓI ' f Ί iP.-'h'OMICCDSRIVVALSSLVSKHWELTNKKYRCMALAEH IS DSI PI S ELS RLR Y N LCb: ϊ: RG H T r ;'Γ; DD SI ;:Ι.·[<Γ I Y h EDDDSSTCSAVTDRETDV (SEQ ID NO. 16) The numbers 1-15 refer to the position of the first amino acid in 15 peptides synthesised, to cover (he BB. C D and DD loop regions of A46. Each peptide contained 11 amino acids from A46. and they overlap by approx 5 amino acids each. Peptides 1-8 surround the predicted BB loop of the protein, and peptides 9-15 cover the CD/DD loop regions. Peptides 1 to 15 have the following sequences: SEQ ID No. Sequence Peptide No. Description 1 GCAVNTPVSMT 1 461 2 TPVSMTYLYNK 2 462 J TYLYNKYSFKL 3 463 4 KYSFKLILAEY 4 464 5 LILAEYIRHRN 5 465 6 YIRHRNTISGN 6 466 7 RNT1SGNIYSA 7 467 8 GNIYSALMTLD 8 468 9 DSGLFDFVNFV 9 469 10 DFVNFVKDMIC 10 4610 il VKDMICCDSRI 11 4611 12 DSR1VVALSSL 12 4612 13 VALSSLVSKHW 13 4613 14 LVSKHWELTNK 14 4614 15 ELTNKKYRCMA 15 4615 In order to deliver these peptides into cells, peptides 1 to 15 were linked to cell-penetrating 10 peptides (also called peptide/protein transduction domains), which are short cationic peptides of normally 8-16 amino acids in length (Murriel & Dowdy, 2006). Cationic peptides are thought to allow delivery of their cargo into ceils by a receptor-independent, fluid-phase macropinocytosis (Murriel & Dowdy. 2006). Once peptide-enclosed macropinosomes enter the cytoplasm, low pH /£ 0 9 0 8 7 5 -42siimulates endosomal release of the peptide-conjugated cargo (Murriel & Dowdy. 2006). Common cell-penetrating peptides employed include the amino acids 47-57 of HIV TAT (YGRKKRRQRRR) (SEQ ID No. 34), amino acids 43-58 of the antcnnapedia homeotic transcription factor (Ant, RQIK1WFQNRRMKWKK) (SEQ ID No. 35) and synthetic peptide carriers such as polyarginine (for example containing nine R residues, here termed 9R) (SEQ ID No. 33) (Murriel & Dowdy, 2006). As the rate of cellular uptake ofthe 9R peptide (SEQ ID No. 33) has been shown to be significantly faster than TAT (SEQ ID No. 34) or Ant (SEQ ID No. 35) for cells in vitro (Wender et al, 2000), the 9R peptide (SEQ ID No. 33) was selected to be fused lo tbe A46-derived peptides (SEQ ID No. 1 to 15) in order to test their ability to inhibit TLR signaling in vitro. Wender et al (2000) showed that a polyarginine peptide containing only 6. 7 or 8 Rs is also taken up by cells, although less effectively than 9R (SEQ ID No. 33). 9R (SEQ ID NO. 33) was fused to the C-terminus of the A46-derived peptides (SEQ ID No. 1 to 15). The sequences of the peptides therefore were: A461: GCAVNTPVSMTRRRRRRRRR (SEQ ID No. 17) A462: I PVSMTYLYNKRRRRRRRRR (SEQ ID No. 18) A463; IYLYNKYSFKLRRRRRRRRR (SEQ ID No. 19) A464: KYS1 KLILAEYRRRRRRRRR (SEQ ID No. 20) A465: L1LAEYIRHRNRRRRRRRRR (SEQ ID No. 21) A466: YIRIIRNT1SGNRRRRRRRRR (SEQ ID No. 22) A467: RNTISGNIYSARRRRRRRRR (SEQ ID No. 23) Λ468: GNIYSALMTLDRRRRRRRRR (SEQ ID No. 24) A469: DSGld DFVNFVRRRRRRRRR (SEQ ID No. 25) A4610: DFVNFVKDMICRRRRRRRRR (SEQ ID No. 26) Λ4611: VKDMICCDSR1RRRRRRRRR (SEQ ID No. 27) A4612: DSRIVVALSSLRRRRRRRRR (SEQ ID No. 28) Λ4613: VALSSLVSKHWRRRRRRRRR (SEQ ID No. 29) Λ4614: LVSKH WELTNKRRRRRRRRR (SEQ ID No. 30) A4615: EL I NKKYRCMARRRRRRRRR (SEQ ID No. 31) (The peptides were named as A46X where X represents the peptide number from the order in which it appears in SEQ ID No. 16) -43IE 0 9 0 8 7 5 These peptides were initially screened for effects on TLR-induced gene induction io vitro using the murine macrophage cell line RAW264.7. Unmethylated CpG dinucleotides of bacterial DNA sequences are detected by TLR9, which leads to production of pro-inflammatory cytokines and chemokines. including TNFot via a MyD88-dependent pathway (Latz et al. 2004 ). RAW264.7 cells showed very potent cytokine production when stimulated with 1 pg/ml CpG (Fig. 1). The effect ol'the peptides on two TLR9-induced cytokines, namely TNFa and MIP-2, was measured by ELISA. TNFa is NFxB-dependent, and MIP-2 as well as NFkB also requires IRFs for transcriptional activation (De Filippo et al. 2008). The cells were plated out at 1x10'^ cells/ml 24 hours before treatment Peptides were added at concentrations of 5 and 20 μΜ 1 hour before stimulation with CpG and supernatants were collected 24 hours post-CpG stimulation lor MIP-2 and mTNFo measurement. This showed that only A463 (SEQ ID No. 19) inhibited CpG-induced MIP-2 secretion (Fig, 1), while only A469 (SEQ ID No. 25) inhibited TNFa production (Fig, 2).
LPS is a component of a cell wall of Gram-negative bacteria which is recognised by a homodimer of' TLR4 and signals to NFkB via both a MyD88- and TRIF-dependent pathway (O'Neill and Bowie, 2007). In order to examine the effect of the peptides on TLR4-dependcnt gene induction in human cells, the human monocytic lymphoma cell line LI937 was used. U937 cells were differentiated into macrophages by treatment with 200nM PMA for 24 hours before any further manipulations. The A46 peptides (SEQ ID No. 17 to 31) were added to the cells as described for RAW264.7 above and the cells were stimulated with 100 ng/ml LPS for 24 hours. In this case. A464 (SEQ ID No. 20) was found to potently inhibit TLR4-mediated TNFa production, while A4610 (SEQ ID No. 26) was also effective, but less so (Fig. 3).
A more detailed investigation of A463 (SEQ ID No. 19), A464 (SEQ ID No. 20), A469 (SEQ ID No. 21) and A4610 (SEQ ID No. 22) was undertaken, and this showed that generally A463 (SEQ ID No. 19), A469 (SEQ ID No. 21) and A4610 (SEQ ID No. 22), as well as inhibiting TLRs, also reduced cell viability (as assessed by the MTT assay, which measures activity of mitochondrial reductase, which can be related to the number of living cells (Domart-Coulon et aL 2000; Elenneke et al. 2002). Thus, future work focused on A464 (SEQ ID No. 20). which was found to be less toxic, and to be more potent than the other peptides at lower doses.
Example 2 - A464 is an inhibitor of murine and human TLR4. -44/£ 0 9 0 8 7 5 A464 (SEQ ID No. 20) showed a very potent inhibitory effect on LPS (TLR4)-induced I N Fa production in U937 human cells, as shown in Fig. 4, and little or no effect on CpG (TLK9)induced cytokine production (Fig. 1A and Fig. 2A).
A52 is another vaccinia virus protein which can inhibit TLR signaling, and it has been shown that a peptide containing 11 amino acids of the A52 sequence together with a 9R delivery sequence (termed PI3, SEQ ID NO. 61) can reduce in vivo bacterial-induced inflammation in a murine model (McCoy et al, 2005). Therefore the ability of A464 (SEQ ID No. 20) and P13 (SEQ ID NO. 61) to inhibit TLR4-induced cytokine production in human cells was compared. For this, the human acute monocytic leukemia cell line THP-1, which is widely used to study LPS responses, was employed. THP-1 cells were stimulated with 100 ng/ml LPS. In this experiment the supernatants were collected 6 hours after stimulation, because TNFa production by these cells was shown to be highest after only 4-6 hours post-stimulation (Takashiba et al, 1999). In tbe majority of the further described ELISA experiments assaying for TNFa, the supernatants were harvested 6 hours after stimulation, unless otherwise stated. Here and for following experiments, A467 (SEQ ID No. 23) was used as a control peptide, since we found it to be inert towards TLRs in every cell type and TLR pathway tested. As before 20 μΜ Λ464 (SEQ ID No, 20) inhibited TLR4-mediated TNFa production (Fig 4). Surprisingly however. Pl3 (SEQ ID NO. 61). even at a high dose of 40 μΜ, was ineffective at inhibiting TLR4 (Fig 4). In fact we consistently found that Pl 3 (SEQ ID NO. 61) was unable to block TLR responses in human cells, suggesting that any in vivo effects of P13 (SEQ ID NO. 61) might be restricted to the murine system. In contrast. A464 (SEQ ID No. 20) was inhibitory against TLR4 in both human (Fig 4) and murine cells. Fig 5 shows that A464 (SEQ ID No. 20) potently inhibited LPS-induced murine TNFa production in RAW264.7 cells at just 5μΜ (Fig. 5A). A simultaneous Μ Π assay showed no toxicity at the concentrations of 5 and 20μΜ in the presence of LPS, and some cytotoxicity at 20 μΜ in the absence of LPS (Fig. 5B).
Next, lower doses of A464 (SEQ ID No. 20) were tested for efficacy against TLR4 in murine cells, compared to the other A46-derived peptides. As shown in Fig. 6, only A464 (SEQ ID No. 20), and not A461 (SEQ ID No. 17), A462 (SEQ ID No. 18), A463 (SEQ ID No. 19). A465 (SEQ ID No. 21). Λ466 (SEQ ID No. 22). A467 (SEQ ID No. 23), A468 (SEQ ID No. 24). A469 (SEQ ID No. 25). A4611 (SEQ ID No. 27), A4613 (SEQ ID No. 29) or A4614 (SEQ ID No. 30). inhibited LPS-induced mTNFa at 1 and 5 μΜ. Further, in human THP-1 cells complete te0 9 0 8 7 5 -45inhibilion of TLR4 was achieved at 5 and 10 μΜ peptide with no cytotoxicity (Fig. 7). Seeing such a strong inhibition of the TLR4-induced cytokine production by the peptide, il was interesting to assay the potency of the peptide to inhibit the signal when the cells were stimulated before the peptide was added, and this showed that 5 and 10 μΜ A464 (SEQ ID No. 20) almost completely inhibited LPS-induced hTNFa production even when added 1 hour after the stimulation with LPS (Fig. 8).
Thus far. A464 (SEQ ID No. 20) had been shown to inhibit both murine and human TLR4. but not murine TLR9. In order to determine whether A464 (SEQ ID No. 20) was specific for TLR4, other TLR pathways were next assayed for sensitivity to A464 (SEQ ID No. 20). A scrambled version of z\464 (SEQ ID No. 20) peptide was designed to use as a further control peptide, and this had the sequence: KALS1FYEKYLRRRRRRRRR (ConA464, SEQ ID No. 32) The A464R (SEQ ID No. 20) and ConA464 (SEQ ID No. 32) peptides were assayed for inhibition of I LR2 signalling in TUP-1 cells. Pam3CysK4 (S-(2.3-bis(palmitoyloxy)-(2-RS)-propyl)-N-palmitoyl IE 0 9 0 875 -46A464 (SEQ II) No. 20) was also tested for an effect on NFkB activation via TLR8 in HEK293 cells. TLRS-dependent NFkB activation proceeds via MyD88 (O’Neill and Bowie. 2007). Cells were stimulated with 2.5 gg/ml CL075, a thiazoloquinolone derivative which is a potent synthetic TI.R8 agonist (Gorden et ah 2005). It was found that neither A467 (SEQ ID No. 23) nor A464 (SEQ ID No. 20) had any inhibitory effect on TLR8-signalIing, even at doses of 25 μΜ (Fig 12). Although murine TLR8 is non-functional, the closely related murine TER7 is functional and this was also found to be insensitive to A464 (SEQ ID No. 20) inhibition.
Next, the A464 (SEQ ID No. 20) peptide was tested in primary human cells. Peripheral blood mononuclear cells (PBMCs) were purified from uncoagulated whole blood using the Ficol Gradient method, and seeded in 96 well plates 24 hours before treatment. A464 (SEQ ID No. 20) and A467 (SEQ ID No 23) were then added to the cells at concentrations of 1, 5 and 25 μΜ 1 hour before stimulation with lOng/ml LPS for 6 hours. As shown in Fig. 13Α. 5μΜ A464 (SEQ ID No. 20) peptide almost completely blocked hTNFa production in response to LPS. while Λ467 (SEQ ID No. 23) had no effect at any dose. The MTT assay showed that both peptides were not toxic for these cells at all concentrations used (Fig. 13B).
Thus. A464 (SEQ ID No. 20) is a potent inhibitor of human and murine TLR4. but has little or no inhibitory effect against human TLR2, TLR3 or TLR8, or murine TLR2, TLR3. TLR7 or TLR9. Further, it is capable of inhibiting LPS responses in primary human cells.
Example 3 - Effect of altered stereochemistry and delivery peptide on the inhibitory potential of A464 I’or further development of the peptide as a potential therapeutic agent it was of use to investigate A464‘s (SEQ ID No. 4) behaviour if the orientation of the amino acids were changed from the natural L-(lavarotatory)-form to D-(dextrarotatory)-form, since the D-form increases peptide stability and is therefore often used m vivo. Thus, the D-form of A464 (SEQ ID No. 20) (D-A464) was chemically synthesised and tested for inhibitory effects on LPS-induced TNFa production in primary human PBMCs and immortalized murine macrophages. In PBMCs DA464 still inhibited TNFa, although it was less potent than A464 (SEQ ID No. 20). since it only blocked TNFa secretion at 25μΜ (Fig. 14A), However, in the murine cells D-A464 was as -47te090875 potent as A464 (SEQ ID No. 20), since both demonstrated complete inhibition at ΙμΜ (Fig. 14B).
We also compared the TAT delivery peptide (SEQ ID No. 34) to 9R (SEQ ID No. 33) since although here 9R (SEQ ID No. 33) proved to be very efficient in vitro, there are reports that TAT (SEQ ID No. 34) is more efficient in vivo (Schwarze et al, 1999). Bearing this in mind it was decided to test the TAT delivery sequence (SEQ ID No. 34) for delivering the 464 (SEQ ID No. 4) peptide into primary cells in vitro. Therefore D-A464 TAT (SEQ ID No. 36) and D-A467 I AT (SEQ ID No. 37) peptides with the TAT delivery sequence (SEQ ID No. 34) at the Cterminus rather than 9R (SEQ ID No. 33) were synthesised and tested in primary human and murine cells. In PBMCs D-A464-TAT (SEQ ID No. 36) failed to inhibit LPS-induced hTNFoc production at 1-25 μΜ (Fig. 15A), while in murine cells it still inhibited, but only at 5-25 μΜ (Fig. 15B>.
Next we tested whether attaching the 9R delivery sequence (SEQ ID No. 33) to the N-terminus of the peptide instead of C-terminus would affect the inhibitory potential, since this might further protect the inhibitory amino acids from degradation. Thus new 9RN-A464 (SEQ ID No. 38) and 9RN-A467 (SEQ ID No. 39) peptides with the 9R delivery sequence on N-lenuini were synthesised and tested in primary human and murine cells. It was found that 9RN-A464 (SEQ ID No. 38) peptide inhibited LPS-induced mTNFa more potently than A464 (SEQ ID No. 20) in murine cells, since complete inhibition was observed at ΙμΜ peptide (Fig. 16A). However ibis modification did not improve inhibitory potential of the peptide in the human PBMCs. since inhibition was only observed from 5-25 μΜ (Fig. 16B). Changing the peptide with the 9R at ihe N-terminus to the D-form did not improve but weakened its inhibitory potential. In the murine cells no inhibition at 1 μΜ was now observed (Fig. 17A), while in the human cells it inhibited TNFa only at concentration οί25μΜ (Fig. 17B).
These results demonstrate that the amino acid residues derived from A46 which are represented in 464 (SEQ ID No. 4) can still inhibit TLR4 responses when the stereochemistry ofthe peptide, or the orientation and nature of delivery peptide, are altered. However, in murine cells, attachment of the 9R delivery peptide (SEQ ID No. 33) to the N-terminus rather than the Cterminus improves the potency of inhibition of TLR4.
IE 0 9 0 8 7 5 -48Example 4 - Identifcation of amino acids in A464 critical for inhibition of TLR4 Previously we showed that peptides derived from the regions of A46 surrounding the A464 sequence (SEQ ID No. 20), namely A463 (SEQ ID No. 19) and A465 (SEQ ID No. 21), did not inhibit ILR4 responses (Fig. 3). Since these peptides partially overlapped with A464 (SEQ ID No. 4). it was possible that a shorter sequence within A464 (SEQ ID No. 4) might be critical for 1LR4 inhibition, and that a shorter inhibitory peptide could be designed. Thus four shorter peptides with amino acids removed from either end of the A46-derived amino acids in A464 (SEQ ID No. 4) were designed and synthesized: A464N-1: YSFKLILAEY-9R (SEQ ID No. 40ία peptide with a deletion of the first amino acid from the N-terminus; A464N-2: SFKl.ILAEYOR (SEQ ID No. 41)- a peptide with a deletion of the first two amino acids from the N-term in us; A464C-3: KYSFKL1L-9R (SEQ ID No. 42)- a peptide with a deletion of the last three amino acids from the C-tcrminus; and A464C-6, KYSFK-9R (SEQ ID No. 43)- a peptide with a deletion of the last six amino acids from the C-terminus.
When these peptides were assayed in murine macrophages for the ability to inhibit TLR4, it was found that only the peptide with the deletion of the 6 amino acids from the C-terminus (A464C6) (SEQ ID No. 4.3) lost its ability to inhibit LPS-induced TNFa production (Fig. 18). Other deletions appeared to affect the inhibitory potential of the peptide to a minor extent, since they did not inhibit TNFa production at ΙμΜ but only at 5 μΜ inhibition (Fig. 18). Importantly, the same results were obtained when primary human cells (PBMCs) were used (Fig. 19) A464N-2 (SEQ ID No. 41) and A464C-3 (SEQ ID No. 42) were then assayed for inhibition of EPS- and Poly(l:C)-induced NFkB activation in HEK293 TLR4 and HEK293 TLR3 cells. Both peptides inhibited LPS-induced NFkB activation completely at 1 μΜ; at the same time there was no inhibition of TLR3 signalling to NFkB up to 25 μΜ peptide (Fig. 20). Based on these experiments, the amino acids SFK.L1L (SEQ ID NO. 55) were defined as the ones essential for inhibition by A464 (SEQ ID No. 20).
Next an alanine scan of A464 (SEQ ID No. 20) was performed, which involved synthesis of a scries of A464 (SEQ ID No. 20) peptides with a substitution of each individual amino acid for alanine. Thus. 10 new peptides were designed with the following sequences (position 9 in A464 (SEQ ID No. 20) is already occupied by an alanine): 1. K1 A: AYSFKLILAEY-9R (SEQ ID No, 44) te0 9 0 8 7 5 2. Υ2Λ: KASFKLILAEY -9R (SEQ ID No. 45) 3. S3A: KYAFKLILAEY-9R (SEQ ID No. 46) 4. F4A: KYSAKLILAEY-9R (SEQ ID No. 47) 5. K5A: KYSFALILAEY-9R (SEQ ID No. 48) 6. L6A: KYSFKAILAEY-9R (SEQ ID No. 49) 7. I7A : KYSFKLALAEY-9R (SEQ ID No. 50) 8. L8A : KYSFKLIAAEY-9R (SEQ ID No. 51) 9. L10A: KYSFKLILAAY-9R (SEQ ID No. 52) 10. Y1IA: KYSFKLILAEA-9R (SEQ ID No. 53) These peptides were assayed for LPS-induced cytokine inhibition in primary human and murine cells, and compared to A464R (SEQ ID No. 20). The parental A464 peptide (SEQ ID No. 20) was used at the concentration of 5μΜ as a positive control. The dashed line represents the level of inhibition by parental peptide (SEQ ID No. 20). In the human PBMCs peptides F4A (SEQ ID NO. 47). Κ5Λ (SEQ ID NO. 48), L6A (SEQ ID NO. 49), I7A (SEQ ID NO. 50) and E10A (SEQ ID NO. 52) showed reduced inhibitory potential compared to the parental Λ464 (SEQ ID No. 20) (Fig. 21 A). Of note, substitution of the glutamic acid for alanine in E10A (SEQ ID No. 52) made the peptide insoluble, which was the likely reason for the reduced inhibitory effect. In the murine cells only L6A (SEQ ID No. 49) displayed reduced inhibition compared to A464R (SEQ ID No. 20) at 5 μΜ (Fig. 21B). These results largely correlate with the data obtained using truncated A464 peptides above (SEQ ID No. 44 to 53): namely that (in human cells at least), FKL1 (SEQ ID No. 54) is essential for optimal inhibition of TLR4.
Based on the previous experiments with the deletions and alanine scanning a new shorter version ofthe A464 peptide (SEQ ID No. 20) was synthesised. This new peptide, called sA464 (SEQ ID No. 55). had the sequence SFKLIL, with the 9R delivery sequence attached to either C- or Nterminus. named accordingly SA464C9R (SEQ ID No. 56) or SA464N9R (SEQ ID No, 57). The control A467 peptide (SEQ ID No. 7) underwent similar modifications and its amino acid sequence became T1SGNI (SEQ ID No, 58) with the 9R delivery sequence attached to either Nor C-terminus. SA467C9R (SEQ ID No. 59) or sA467N9R (SEQ ID No. 60). The short A464 (SEQ ID No. 55. 56 and 57) and A467 (SEQ ID No. 58, 59 and 60) peptides were assayed in murine RAW264.7 cells. The peptides were added to the cells at concentrations of 5 and 25μΜ. A464 (SEQ ID No. 20) and A467 (SEQ ID No, 23) were used at concentrations of 5μΜ as a 0 θ ο 8 7 5 -50posilive and negative controls. It was found that sA464 (SEQ ID No. 55) was able to inhibit LPS-induced I N Fa, but only when the delivery sequence was at the N-terminus (SEQ ID No. 57) (Pig. 22). Further SA464N9R (SEQ ID No. 57) inhibited LPS-induced TNFa production as potently as A464 (SEQ ID No. 20) at 5 μΜ, demonstrating that the removal of the Hanking amino acids did not affect the potency of the peptide to inhibit TLR4 at this concentration in murine cells (Fig. 22). Next the effect of even shorter versions of A464 on TLR4-induced murine TNFa in immortalised murine macrophages was tested. The peptide SA464N9R (SEQ ID NO. 57) was further reduced in size with a deletion of one amino acid from either N- terminus (9RFKLIL) (SEQ ID NO. 62) or C-terminus (9R-SFKLI) (SEQ ID NO. 63) or both (9R-FKLI) (SEQ ID NO. 64) and one with substitution of Isoleucine (I) to Phenylalanine (F) (9R-SFKLFL) (SEQ ID NO. 65). This showed that 9R-FKLIL retained the ability to inhibit TLR4. while 9RSFK.LL 9R-FK.LI. or 9R-SFK.LFL did not (Fig 23A). None of these peptides were toxic to cells (Fig. 23B).
Thus the sequence FKLIL (SEQ ID No. 68), or a derivative of it such as a peptidomimelic. is a strong candidate for drug development of a specific TLR4 inhibitor with efficacy in human cells.
Example 5 - A464 inhibits TLR4-dependent signaling pathways (transcription factors and MAP kinases) and interacts with adaptor components of the TLR4 receptor complex Activation of TLR4 by LPS stimulates activation of the transcription factors NFkB and IRFs, as well as MAPKs, such as p38 and JNK, all of which contribute to altered gene expression. A464 (SEQ ID No. 20) was shown to be capable of inhibiting LPS-induced NFkB activation (Fig. 24). In contrast. NFkB activated by forced dimerisation of TLR4 TIR domains in the absence of LPS (by overexpressing the fusion protein CD4-TLR4) was insensitive to inhibition by A464 (SEQ ID No. 20) (Fig. 24). Thus preformed dimers of TLR4 may be insensitive ίο A464 (SEQ ID No. 20) and therefore A464 (SEQ ID No. 20) may act to prevent normal TLR4 dimerisation after LPS stimulation.
To further assay activation of NFkB, the degradation of its inhibitor ΙκΒα. an event required for the translocation of the NFkB into the nucleus, was measured. RAW264.7 cells were seeded at 1.5x10' cells/ml in 12 well plates 24 hours before treatment. A467 (SEQ ID No. 23) and A464 (SEQ ID No. 20) were added to the cells at concentrations of 5μΜ 1 hour before stimulating with lOng/ml LPS for 5, 15, 30 or 60 min. Cells were then harvested and lysed in 1% (v/v) NPIE 0 9 08 75 -51 40 Lysis Buffer (see Materials and Methods). The protein concentration in each sample was determined by Bradford assay and samples were diluted accordingly to ensure equal protein loading onto a 12% (w/v) SDS-PAGE gel. Resolved proteins were transferred from (lie gel onto PVDF membrane by semi-dry transfer and immunoblotted for ΙκΒα. As seen in Fig. 25 Α. ΙκΒα was present in the control untreated samples and in the unstimulated samples treated with A464 (SEQ ID No. 20) or A467 (SEQ ID No. 23). Stimulation with LPS for 5 min did not significantly affect levels ol ΙκΒα in the samples, but after 15 min of LPS treatment ΙκΒα had completely disappeared in the sample treated with A467 (SEQ ID No. 23), and re-appeared again at 60 min treatment, due to rapid re-synthesis of IkB protein (Krappmann and Scheidereit. 1997). 1 towever. A464 (SEQ ID No. 20) blocked the degradation of ΙκΒα seen at 15 and 30 min (Fig. 25A). Re-probing for β-actin protein confirmed that the gel was loaded evenly (Fig. 2513), As well as NFkB. A464 (SEQ ID No. 20) also completely inhibited LPS-induced IRF3 activation at I μΜ in I IF.K293 cells (Fig. 26).
Next, the effect of A464 (SEQ ID No. 20) on MAPK activation was measured by assaying the phosphorylation of p38 and JNK by Western blot. To examine the effect of A464 (SEQ ID No. 20) on the phosphorylation of JNK, RAW264.7 cells were treated with 10 ng/ml EPS lor 5-60 min. The membrane was probed with the antibody specific for phospho-JNK protein and the levels of JNK expression in the samples were checked by re-probing the blot for total JNK. As before the protein concentration in the samples was determined by Bradford assay and the accuracy of the loading was confirmed by re-probing the blot for β-actin. As shown in Fig. 27 there was no phosphorylated JNK detected in the unstimulated samples in the presence of A467 (SEQ ID No. 23) or A464 (SEQ ID No. 20), or in the untreated sample. When stimulated with lOng/ml lor 5 min the phosphorylation of both JNK1 and JNK2 isoforms was detected as two faint bands in the sample treated with A467 (SEQ ID No. 23), which became much stronger at 15 min. and then diminished at 30 and 60 min treatment with LPS. A464 (SEQ ID No. 20) completely inhibited phosphorylation of JNK at all times of the LPS stimulation (Fig. 27 A). The levels of the total JNK protein were equal throughout the all samples (Fig. 27B) and the loading was even (Fig. 27C).
The effect ofthe A464 (SEQ ID No. 20) peptide on p38 MAPK was also examined, and this was of particular interest since p38 has been shown to play an important role in the development of various autoimmune diseases (Kumar et aL 2003), Phosphorylation of p38 was assayed in a /£ 0 9 ο 8 7 5 -52manner similar to the experiment described for INK above. Cells were pretrealcd with no peptide, or with Λ467 (SEQ ID No. 23) or A464 (SEQ ID No. 20) prior to stimulation with 10 ng/ml LPS for 5. 30 or 60 min. As in the previous experiment, A464 (SEQ ID No. 20) showed very potent inhibition of LPS-induced p38 phosphorylation at all the time points of LPS stimulation (Pig. 28A). Fig. 28B shows that the expression of total p38 was equal throughout.
Consistent with TLR4 inhibition by A464 (SEQ ID No. 20) and not A467 (SEQ ID NO. 23), a I lis-tagged version of464 (without the 9R delivery sequence, SEQ ID NO. 66) but not 467 (SEQ ID NO. 67) was capable of pulling down overexpressed Mai or TRAM from cell lysates (Fig. 29). demonstrating the ability of the peptide 464 to interact with critical adaptor components of the active TLR4 receptor complex.
Example 6 - A464 inhibits LPS-induced cytokine production in vivo Given that A464 (SEQ ID No. 20) was shown to be a specific TLR4 inhibitor in both human and murine cells, it was of interest to determine whether a related peptide could block LPS responses in vivo. To do this we used a well characterised model of Gram negative sepsis, where mice are injected with LPS and serum cytokines are later analysed by ELISA. For this, the effect of D9RN-A464 (SEQ ID No. 38) or L-9RN-A464 (SEQ ID No. 38) was compared to L-9RN-A467 (SEQ II) No. 34), since both of these forms of A464 (SEQ ID No. 38) were shown to inhibit 'INF production in murine macrophages (Fig. 16 and 17). Mice were injected i.v. with LPS alone, or with peptide in the presence of LPS. Four hours later serum IL-12p40 concentrations were measured. Fig. 30 shows that both D-9RN-A464 (SEQ ID No. 38) or L-9RN-A464 (SEQ ID No. 38) significantly inhibited LPS-induced IL-12p40 to the extent that mice treated with these peptides displayed levels of IL-12p40 wrhich were not significantly different from the PBStreated animals. In contrast, although the control peptide L-9RN-A467 (SEQ ID No. 39) did display some inhibition of LPS-induced IL-12p40, it failed to cause as dramatic a reduction as the A464 peptides (Fig. 30). Thus peptides based on the 464 sequence (SEQ ID No. 4) have efficacy in vivo. Thus peptides comprising the 464 sequence (SEQ ID No.4) can inhibit LPS induced proinflammatory !L-12p40 in vivo.
We have shown that A464 (SEQ ID No. 20) inhibits TLR4-induced MAP kinase activation, transcription factor activation and induction of gene expression leading to cytokine and interferon production. Therefore biological responses, including but not limited lo MAP kinase activation, transcription factor activation and gene induction, that are inhibited by administration -53IE 0 9 08 75 of Λ464 (SEQ ID No. 20) would be expected to be TLR4-dependent. In this way. in vitro treatment of cells in culture or in vivo treatment of a whole animal with Λ464 (SEQ ID No. 20). and measuring alterations in biological responses perturbed by the administration of A464 (SEQ ID No. 20) compared to control cells or an untreated animal, can be used as a method to implicate TI.R4 in such a biological response. Thus a pathogen- or host-derived substance which induces a biological response in cells or animals could be tested for its ability to activate TLR4 in the presence and absence of A464 (SEQ ID No. 20). In this way compounds or substances that activate TLR4 could be identified and such activators may have use as adjuvants and boosters of an immune response.
LPS signals via TLR4 in order to induce its proinflammatory effects. TLR4 has been implicated in many diseases in man. In autoimmune and inflammatory conditions, endogenous ligands for TLRs have been implicated in their pathogenesis. Although LPS is not be the causative agent of many of these diseases, it follows that potential endogenous ligands that signal via TLR4 could also be blocked using this approach, as 464 (SEQ ID No. 4) peptides block internally.
TLR antagonists hold promise as therapeutics in immune and inflammatory diseases (Kanzler at uL 2007). further, TLR4 inhibitors warrant particular interest for use in the prophylaxis and/or treatment of severe sepsis, which kills more than 200,000 people in the US each year (Lolis and Bucala. 2003). Sepsis results from uncontrolled activation of LPS/TER4-indueed cytokine induction, and is also amplified by the endogenous TLR4 activators myeloid-related protein-8 (Mrp8) and Mrpl4 (Vogl et al, 2007). Mrp8 and Mrpl4 are cytosolic proteins in neutrophils and monocytes whose expression are strongly upregulated in inflammatory diseases such as sepsis, rheumatoid arthritis, inflammatory bowel disease and cancer. Mrp8 and Mrpl4 are secreted by activated phagocytes and were shown to have a significant role in the pathogenesis of sepsis and to promote lethality in a murine septic shock model. Mrp8 bound directly to the TLR4-MD2 complex to mediate its effects (Vogl et al, 2007). Thus a specific TLR4 inhibitor would be expected to have a significant protective effect if administered to patients at risk of sepsis. Traditionally. attempts to control sepsis have centred on blockage of pro-inflammatory cytokines sueh as TNHx. a presumed critical effector of LPS/TLR4 toxicity. However TLR.4 itself may be a much more effective target for intervention in sepsis, since cellular activation by Mrp8-TLR4 amplifies inflammation (Vogl et al, 2007). /£ 0 9 0 8 7 5 -54Apart from sepsis, specific inhibition of TLR4 while leaving other pathogen detection pathways intact would have great therapeutic potential in a number of other diseases. Sterile inflammation, which is caused by chemical insult and / or tissue damage rather than by pathogens, may also be mediated by TLR4 in certain contexts such as bleomycin-induced lung inflammation (Kanzler at ai, 2007). TLR4 has also been implicated in kidney ischemia/reperfusion injury (IRI). since in a murine model of IRI, mice lacking TLR4 were protected against kidney dysfunction, tubular damage, neutrophil and macrophage accumulation in the kidney, and cytokine production after ischemia (Wu et ai, 2007). Further, TLR4 may also have a role in liver IRI (Zhai et al, 2004). in ischemic brain injury (Tang et al, 2007) and in plaque development in atherosclcrosis-prone apolipoprotein h-deficient mice (Michelsen et al, 2004). Acute lung injury (AIJ). for which treatment options are currently limited and which is a leading cause of death in human I-I5NI avian influenza infections, is also TLR4 dependent. ALI is triggered by oxidized phospholipids in the lung which stimulate TLR4 signalling through the TRIF-dependent pathway, leading to cytokine production (Imai et al, 2008).
Given the important role of TLR4 in disease pathogenesis, the development of specific TLR4 inhibitors is important. Viral immunosuppressive proteins have been finely-tuned and honed by evolution to target the host immune system with maximal effectiveness. This is analogous to a 'naturally occurring drug development programme', whereby the protein has already undergone cycles of modification due to natural selection, leading to enhanced inhibitory function. Vaccinia virus has developed effective ways of inhibiting TLRs. Thus peptides derived from such proteins may more potently and specifically target TLR-mediated inflammation compared to current nonspecific therapeutic strategies.
I'hc Λ46 protein, when expressed in human or murine cells, has been shown to inhibit activation of NI'kB (which is a critical transcription factor in mediating inflammation) in response to IL-1 (Bowie et al, 2000), to TLR agonists including TLR4 (Stack et al, 2005; Aravalli et al, 2007). and in murine cells to HSV infection (Aravalli et al, 2007). Furthermore, A46 can also block IL1 and TLR-mediated MAP kinase and IRF activation in human cells (Stack et al, 2005). A46 works by binding to TIR domains in host proteins, and has been shown to be able to associate with TLR4. IL-lRAcP, MyD88, Mai. TRIF and TRAM, which explains how A46 can inhibit multiple Tl .R pathways (Stack et al, 2005). In contrast, the effects of the A464 peptide (SEQ ID No. 20) arc specific to inhibition of TLR4 signalling. A46 protein inhibits TLRs by interacting with TIR domains, it is likely that the A464 peptide (SEQ ID No. 20) acts in a similar manner to -55fe090875 preveni critical TIR-TIR interactions in TLR4 signalling, TLR4 signalling involves live distinct TIR-domain containing proteins: TLR4, Mai, MyD88, TRAM and TRIF. Given that A464 (SEQ ID No. 20) did not inhibit TLRs which use TRIF (TLR3) or MyD88 (TLR9) it is unlikely that the peptide targets either of these two adaptors directly. Rather it is likely that A464 (SEQ ID No. 20) targets ILR4, Mai or TRAM in order to disrupt TLR4-TLR4 and/or TLR4-TRAM and/or TLR4-MaI T1R interactions. Consistent with this, His-tagged A464 (SEQ ID NO. 66) was capable of interacting with overexpressed Mai or TRAM when added to cell lysates.
It is likely that Λ464 is binding to a novel site on Mai or TRAM that is essential for TLR4 function. Therefore the A464 or related peptides may be useful in screening for novel inhibitors of TER4 or Mai or TRAM. Assuming that A464 binds to a site on Mai or TRAM essential for TLR4 function, a screen could be established to assay for small molecules that would bind to this site and thus displace A464. For example, FITC-labelled A464 displacement from recombinant TRAM or recombinant Mai could be measured as an assay of small molecule binding to said site.
The invention is not limited to the embodiment hereinbefore described, with reference to the accompanying drawings, which may be varied in construction and detail.
IE 0 9 Ο 8 7 5 -56Referenees Akira S. Uematsu S, Takeuchi O., 2006. Pathogen recognition and innate immunity. Cell 124. 783-801.
Ansel's Pharmaceutical Dosage Formsand Drug Delivery Systems; Allen. L.V., Papovich N.G and Ansel. H.C. 2005 8,h Edition ISBN 0-781746-12-4, ppl85-505; 653-671.
Aravalli ei ah. 2007. Inhibition of Toll-like Receptor Signaling in Primary Murine Microglia.7 Neuroimmune Pharmacol doi 10.1007/s 11481 -007-9097-8 Bowie cl (th. 2000. A46R and A52R from vaccinia virus are antagonists of host IL-l and Tolllike receptor signaling. Proc Natl Acad Sci USA 97, 10162-10167 Dc Filippo ci ah. 2008. Neutrophil Chemokines KC and Macrophage-Inflammatory Protein-2 Are Newly Synthesized by Tissue Macrophages Using Distinct TER Signaling Pathways. 7/m/mm<7180,4308-4315.
Domart-Coulon cl ah, 2000. Cytotoxicity assessment of anti biofoul ing compounds and byproducts in marine bivalve cell cultures. Toxicol In Vitro 14, 245-251.
Gorden cl al.. 2005. Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8. J Immunol 174, 1259-1268.
Iarte, M l ci ah 2003 The poxvirus protein A52R targets Toll-like receptor signalling complexes to suppress host defence. J Exp. Med. 197,343-351.
Henneke et al.. 2002. Cellular Activation, Phagocytosis, and Bactericidal Activity Against Group B Streptococcus Involve Parallel Myeloid Differentiation Factor 88-Dependeiu and Independent Signaling Pathways. J Immunol 169, 3970-3977.
Imai et ah. 2008. Identification of Oxidative Stress and Toll-like Receptor 4 Signaling as a Key Pathway o ('Acute Lung Injury. Cell 133,235-249.
Kanzler et al.. 2007. Therapeutic targeting of innate immunity with Toll-like receptor agonists and antagonists. Nat Medicine 13, 552-559.
Keating. SE. Maloney, GM, Moran, EM, Bowie, AG, 2007. IRAK-2 participates in multiple tolllike receptor signalling pathways to NFliB via activation of TRAF6 ubiquitination. J. diol Chem. 282,33435-33443.
Krappmann and Scheidereit, 1997. Regulation of NF-kappa Β activity by 1 kappa B alpha and I kappa B beta stability. Immunobiology' 198. 3-13.
Kumar cl al.. 2003. p38 MAP kinases; key signalling molecules as therapeutic targets for inflammatory diseases. Nat Rev Drug Discov 2, 717-726.
Langer et a 1.7. Bio tne d. Mater. Res. 15: 167-277. 1981 Langer. ( hem. Tech. 12:98-105, 1982 -57te 0 9 08 75 Latz ef ui.. 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
Nat Immunol 5. 190-198.
LoiarroiVi//.. 2005. Peptide-mediated Interference ofTIR Domain Dimerization in MvD88 Inhibits Interleukin-1 -dependent Activation of NF-KB. J. Biol. Chetn. 280, 15809-15814. koi is and Bueala, 2003. Therapeutic approaches to severe sepsis Nat Rev Drug Discov 2.635645.
McCoy ef of. 2005. Identification of a Peptide Derived from Vaccinia Virus A52R Protein That inhibits Cytokine Secretion in Response to TLR-Dependent Signaling and Reduces In Vivo Bacterial-Induced Inflammation. J. Immunol. 174, 3006-3014.
Michel sen et al., 2004. Lack of Toll-like receptor 4 or myeloid differentiation factor 88 reduces atherosclerosis and alters plaque phenotype in mice deficient in apolipoprotein Γ Proe Nail Acad Set USA 101, 10679-10684.
Murriel & Dowdy, 2006. Influence of protein transduction domains on intracellular delivery of macro molecules. Expert Opin.Drug Deliv. 3. 739-746.
O'Neill. I ,AJ. 2006. Targeting signal transduction as a strategy to treat inflammatory diseases. Nature Rew Drug Disc. 7, 549-563.
O'Neill. LA.L Bowie, AG, 2007. The family of five: TIR-domain-containing adaptors in Tolllike receptor signalling. Nat. Reviews Immunol. 7,353-364.
Schwarze el al.. 1999. In vivo protein transduction: delivery of a biologically active protein into Ihe mouse. Science 285, 1569-1572.
Sidman et al. Biopolymers 22(1): 547-556. 1985 Stack. .1 ef al. 2005. Vaccinia virus protein A46R targets multiple Toll-like-interleukin-1 receptor adaptors and contributes to virulence. J. Exp. Nied. 201, 1007-1018 Takashiba et al.. 1999. Differentiation of monocytes to macrophages primes cells for lipopo I y saccharide stimulation via accumulation of cytoplasmic nuclear factor kuppaB. Infect fmmun 67, 5573-5578.
Tang et al.. 2007. Pivotal role for neuronal Toll-like receptors in ischemic brain injury and functional deficits. Proc Natl Acad Sci USA 104,13798-13803, Toshchakov ef al., 2005. Differential Involvement of BB Loops of Toll-IL-1 Resistance (TIR) Domain-Containing Adapter Proteins in TLR4- versus TLR2-Mediated Signal Transduction. J. Immunol 175,494-500.
Toshchakov ef al., 2007. Cutting Edge: Differential Inhibition of TLR Signaling Pathways by Cell-Permeable Peptides Representing BB Loops of TLRs. J. Immunol 178, 2655-2660.
Toshchakov and Vogel, 2007. Cell-penetrating TIR BB loop decoy peptides a novel class of -58IE 0 9 0 8 7 5 TLR signaling inhibitors and a tool to study topology of TIR-TIR interactions. Expert Opin. Rial. Ther. 7, 1035-1050. Ί sung et oh. 2007. A novel inhibitory peptide of toll-like receptor signaling limits (ipopolysaccharide-induced production of Inflammatory mediators and enhances survival in mice. Shock 27, 364-369 Vogl et ah. 2007, Mrp8 and Mrpl4 are endogenous activators of Toll-like receptor 4. promoting lethal, endotoxin-induced shock. Nat Medicine 13, 1042-1049 Wender et oi.. 2000. The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters. Proc Nad Acad Sci USA 97, 1300313008.
Wu el a 1.. 2007. TLR4 activation mediates kidney ischemia/reperfusion injury. J CUn invest 117. 2847-2859.
Zhai et ah. 2004. Cutting Edge: TLR4 Activation Mediates Liver Ischemia/Reperfusion Inflammatory Response via IFN Regulatory Factor 3-Dependent MyD88-lndependent Pathway.,/ Immunol 15, 7115-7119.
US Patent No. 3, 773,919 EP-A-0058481 /£ 0 9 0 8 7 5

Claims (10)

Claims
1. Λ peptide lor inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID No. 69,
2. Λ peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID No. 68.
3. Λ peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino aeid sequence of SEQ ID NO. 4, SEQ ID NO 55, SEQ ID NO 68, SEQ ID NO. 69, SEQ ID NO 70. SEQ ID NO 71, SEQ ID NO 72. SEQ ID NO 79, SEQ ID NO 82. SEQ ID NO 85. SEQ ID NO 88, SEQ ID NO 91, SEQ ID NO 94, SEQ ID NO 97, SEQ ID NO 100. SEQ ID NO 103, SEQ ID NO 106, SEQ ID NO 109, SEQ ID NO 112. or SEQ ID NO 115. 4. SEQ ID No. 55, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 79, SEQ ID No. 82, SEQ ID No. 85, SEQ ID No. 88. SEQ ID No. 91, SEQ ID No. 94, SEQ ID No. 97, SEQ ID No. 100, SEQ ID No. 103. SEQ ID No. 106. SEQ ID No. 109, SEQ ID No. 112, or SEQ ID No. 115 to a subject. 31. A method of inhibiting TLR4 induced responses comprising the step of administering an effective amount of a pharmaceutical composition comprising a peptide having the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ II) No. 41. SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62. SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77. -63te0 9 0 8 7 5 SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No, 8k SEQ ID No. 82. SEQ ll) No. 83, SEQ ID No. 84, SEQ ID No.85. SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88. SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93. SEQ ID No, 94, SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98. SEQ ID No, 99. SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103. SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109.SEQ ID No. 110, SEQ ID No. Ill,SEQ ID No. 112. SEQ ID No. 113.SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, SEQ ID No. 117, or peptidomimetic thereof and a pharmaceutically acceptable excipient to a subject. 32. A method of suppressing a pro-inflammatory immune response comprising the step of administering an effective amount of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42. SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68. SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72. SEQ ID No. 73. SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78. SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83. SEQ ID No. 84. SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88. SEQ ID No. 89. SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94. SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99. SEQ ID No. 100. SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SI-Q ID No. 105. SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109. SEQ ID No. 110. SEQ ID No. Ill,SEQ ID No. 112, SEQ ID No. 113,SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116, SEQ ID No. 117, or peptidomimetic thereof to a subject. 33. Use of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42. SEQ ID No. 55, SEQ II) No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No, 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74. SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77. SEQ ID No. 78, SEQ ID No.79. SEQ ID No, 80. SEQ 11) No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84. SEQ ID No.85. SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95. SEQ II) No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. -64SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106. SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. Ill, SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116. or SEQ ID No. 117 to suppress an immune response wherein the immune response is mediated through the stimulation ofTLR4 leading to the activation ofa MAP kinase, or at least one transcription factor selected from NF-κΒ and at least one IRF. 34. Use as claimed in claim 33 wherein the IRF is IRF3 or IRF7. 35. Use ofa peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20. SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55. SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84. SEQ II) No.85. SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90. SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93. SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106. SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110. SEQ ID No. 111. SEQ ID No. 112,SEQ ID No. 113, SEQ ID No. 114, SEQIDNo. 115. SEQIDNo. 116. or SEQ ID No. 117 in the preparation of a medicament for down regulating a TLR4mediated immune response. 36. Use as claimed in claim 35 wherein the immune response is mediated through the activation of at least one MAP kinase or a transcription factors selected from NF-κΒ and at least one IRF. 37. Use as claimed in claim 36 wherein the IRF is IRF3 or IRF7. 38. A pharmaceutical composition comprising a therapeutically effective amount ofa peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20. SEQ ID No. 38. SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62, SEQ ID No. 68, SEQ II) No. 69. SEQ ID No. 70. SEQ ID 09 087 5 -65No. 71. SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75. SEQ ID No. 76. SEQ ID No. 77, SEQ ID No. 78. SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83. SEQ ID No. 84, SEQ ID No.85. SEQ ID No. 86, SEQ II) No. 87. SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92. SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97. SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. SEQ ID No. 102. SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105, SEQ ID No. 106. SEQ ID No. 107. SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. 111, SEQ ID No. 112. SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116. or SEQ ID No. 117 and a pharmaceutically acceptable diluent, excipient or carrier. 39. A method of prophylaxis and/or treatment of an immune-mediated condition comprising the step of administering an agent comprising a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40. SEQ ID No. 41. SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72. SEQ ID No. 73, SEQ ID No, 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77. SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87. SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98. SEQ ID No. 99. SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103. SEQ ID No. 104. SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108. SEQ ID No. 109. SEQ ID No. 110, SEQ ID No. Ill, SEQ ID No, 112, SEQ ID No. 113. SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116, SEQ ID No. 117, or peptidomimetic thereof to a subject wherein administration of the agent suppresses the activation of a MAP kinase or the transcription factors NF-κΒ and at least one IRF. 40. A method as claimed in claim 39 wherein the IRF is IRF3 or IRF7. 41. A method as claimed in claim 39 or 40 wherein the immune mediated disorder is an undesirable or aberrant immune response triggered by the activation of TLR4. -66#090875 42. Λ method as claimed in claim 41 wherein the immune response is directed to a self' antigen. 43. A method as claimed in claim 41 or 42 wherein the immune response is physiologically normal but is undesirable, 44. A method as claimed in any one of claims 39 to 43 wherein the immune mediated condition is one or more selected from the group comprising: multiple sclerosis, rheumatoid arthritis, Crohn’s disease, psoriasis, SLE, lupus, type 1 diabetes, colitis, inflammatory bowel disease, asthma, allergy diabetes mellitus, myasthenia gravis, systemic lupus erythematosis, autoimmune thyroiditis, dermatitis (including atopic dermatitis and eczematous dermatitis), Sjogren's Syndrome. including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, alopecia areata, allergic responses due to arthropod bite reactions, aphthous ulcer, iritis, conjunctivitis, keratoconjunctivitis, ulcerative colitis, cutaneous lupus erythematosus, scieroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, allergic encephalomyelitis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis. Wegener's granulomatosis, chronic active hepatitis, Stevens-Johnson syndrome, idiopathic sprue, lichen planus, Graves ophthalmopathy, sarcoidosis, primary biliary cirrhosis, uveitis posterior, interstitial lung fibrosis, Alzheimer’s disease and coeliac disease, or atopic disease. 45. Λ method as claimed in any one of claims 39 to 44 wherein the immune-mediated condition is an autoimmune disease. 46. Λ method as claimed in claim 45 wherein the autoimmune disease is one or more selected from the group comprising: multiple sclerosis, rheumatoid arthritis, Crohn’s disease, psoriasis, SLE, lupus, type I diabetes, colitis, inflammatory bowel disease, asthma and allergy. 47. A method for down regulating an immune response of a subject following tissue trunsplantion comprising the step of administering an agent comprising a peptide fB 0 9 0 8 7 5 -67comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20. SEQ ID No. 38. SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No, 55, SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71. SEQ ID No. 72, SEQ ID No. 73. SEQ ID No. 74, SEQ ID No. 75. SEQ ID No. 76. SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81. SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85. SEQ ID No. 86. SEQ ID No. 87. SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92. SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97. SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No, 105, SEQ ID No. 106, SEQ ID No. 107. SEQ ID No, 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. 111. SEQ ID No. 112. SEQ ID No. 113, SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116. SEQ ID No. 117. or peptidomimetic thereof to a subject. 48. A method of modulating intracellular signalling mediated by TLR4 comprising the step of administering a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20. SEQ ID No, 38, SEQ ID No. 40. SEQ ID No. 41, SEQ ID No. 42. SEQ ID No. 55. SEQ ID No, 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68. SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74. SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79. SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84. SEQ ID No.85. SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90. SEQ ID No. 91. SEQ ID No. 92, SEQ ID No, 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100. SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105. SEQ ID No. 106. SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110. SEQ ID No. 111, SEQ ID No. 112, SEQ ID No. 113. SEQ ID No. 114, SEQ ID No. 115. SEQ ID No. 116. or SEQ ID No. 117 to a subject. 49. Use of an A464 peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20. SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55. SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75. SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79. SEQ ID No. /£ 0 9 08 75 -6880. SEQ ID No, 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90. SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98. SEQ ID No. 99, SEQ ID No. 100. SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105. SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. 111. SEQ ID No. 112, SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115. SEQ ID No. 116. or SEQ ID No. 117 to modulate intracellular signalling mediated by TLR4 following the binding of a suitable agonist. 50. A method for identifying a compound and/or substance suitable for modifying the biological activity of TLR4 comprising the steps of: (a) contacting a biological sample with a compound and/or substance to be tested in the presence of a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41. SEQ ID No. 42, SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72. SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No, 77. SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92, SEQ ID No 93, SEQ ID No. 94, SEQ ID No. 95, SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98, SEQ ID No, 99, SEQ ID No, 100. SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104. SEQ ID No. 105. SEQ ID No. 106, SEQ ID No. 107. SEQ ID No. 108, SEQ ID No. 109. SEQ ID No. 110, SEQ ID No. Ill,SEQ ID No. 112,SEQ ID No. 113,SEQ ID No. 114. SEQ ID No. 115, SEQ ID No. 116, or SEQ ID No. 117; (b) assaying the biological sample for a biological response; and (e) comparing the biological response of a sample contacted with a compound and/or substance in the presence of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40. SEQ ID No. 41. SEQ ID No. 42, SEQ ID No. 55. SEQ ID No. 56, SEQ ID No. 57. SEQ ID No. 62. SEQ ID No. 68, SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 75, SEQ ID No. 76. SEQ IE 0 9 0 8 7 5 -69ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 8E SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85. SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90. SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94. SEQ ID No. 95. SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99. SEQ ID No. 100. SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104. SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. 111, SEQ ID No. 112, SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116, or SEQ ID No. 117 to the biological response of a sample contacted with a compound and/or substance in the absence ofthe peptide. 51. A method as claimed in claim 50 wherein the biological response is one or more of MAP kinase activation, transcription factor activation and gene induction. 52. A method as claimed in claim 50 or 51 wherein the biological response is inhibited bv the presence of a peptide comprising the amino acid sequence of SEQ ID No. 4. SEQ ID No. 20. SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 55, SI-Q ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 68, SEQ ID No. 69. SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73, SEQ ID No. 74. SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80. SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84, SEQ ID No.85, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 91. SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94, SEQ ID No. 95. SEQ ID No. 96. SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101. SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104, SEQ ID No. 105. SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109, SEQ ID No. 110, SEQ ID No. 111. SEQ ID No. 112, SEQ ID No. 113, SEQ ID No. 114, SEQ ID No. 115, SEQ ID No. 116. or SEQ ID No. 117. 53. A method as claimed in any one of claims 50 to 52 wherein the biological sample is cultured cells. fe090875 -7054. A method as claimed in any one of claims 50 to 52 wherein the biological sample is a non-human animal. 55. Use of a compound and/or substance identified by the method of any one of claims 50 to
4. Λ peptide as claimed in any one of claims 1 to 3 wherein the amino acid sequence is in the L-t'orm. 5. 54 as an adjuvant and/or booster of an immune response. 56. A vaccine comprising a compound and/or a substance identified by the method of any one or claims 50 to 54.
5. A peptide as claimed in any one of claims 1 to 3 wherein the amino acid sequence is in the D-lorm.
6. Λ peptide as claimed in any one of claims 1 to 5 comprising a delivery sequence.
7. Λ peptide as claimed in claim 6 wherein the delivery sequence is a cationic peptide.
8. A peptide as claimed in claim 6 or 7 wherein the delivery sequence is between 8 and 16 amino acids in length.
9. A peptide as claimed in any one of claims 6 to 8 wherein the delivery sequence comprises the amino acid sequence of SEQ ID NO. 33, SEQ ID NO. 34, or SEQ ID NO. 35. 10. A peptide as claimed in any one of claims 6 to 9 wherein the delivery sequence is attached to the C terminus of the peptide. IE 0 9 0 8 7 5 -6011. A peptide as claimed in claim 10 comprising an amino acid sequence selected from the group comprising: SEQ ID No. 20, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42, SEQ ID No. 56, SEQ ID No. 73, SEQ ID No. 74, SEQ ID No. 80. SEQ ID No. 83, SEQ ID No. 86. SEQ ID No. 89, SEQ ID No. 92, SEQ ID No. 95, SEQ ID No. 98, SEQ ID No 101. SEQ ID No. 104, SEQ ID No. 107, SEQ ID No. 110, SEQ ID No. 113. and SEQ ID No. 116. 12. A peptide as claimed in any one of claims 6 to 9 wherein the delivery sequence is attached to the N terminus of the peptide. 13. A peptide as claimed in claim 12 comprising an amino acid sequence selected from the group comprising: SEQ ID No. 38, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 75. SEQ ID No. 76. SEQ ID No. 77, SEQ ID No. 78, SEQ ID No. 81, SEQ ID No. 84. SEQ ID No. 87. SEQ ID No. 90, SEQ ID No. 93, SEQ ID No. 96, SEQ ID No. 99, SEQ ID No. 102. SEQ ID No. 105, SEQ ID No. 108, SEQ ID No. Ill, SEQ ID No. 114. and SEQ ID No. 117. 14. A peptide for inhibiting Toll-like receptor 4 (TLR4) signalling comprising the amino acid sequence of SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 42. SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62, SEQ ID No. 73, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 86. SEQ ID No. 87, SEQ ID No. 89, SEQ ID No. 90, SEQ ID No. 92, SEQ ID No. 93. SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 104. SEQ ID No. 105. SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 110, SEQ ID No.llLSEQ ID No.116, or SEQ ID No.117 15. A peptide comprising the amino acid sequence of SEQ ID NO. 20 or a fragment, analogue or derivative thereof. 16. A peptide comprising the amino acid sequence of SEQ ID NO. 62 or a fragment, analogue or derivative thereof 17. A peptide comprising the amino acid sequence of SEQ ID NO. 38 or a fragment, analogue or derivative thereof. IE 0 9 0 8 7 5 -61 18. A peptide comprising the amino acid sequence of SEQ ID NO. 73 or a fragment, analogue or derivative thereof. 19. A peptidomimetic for inhibiting Toll-like receptor 4 (TLR4) signalling based on a peptide as claimed in any one of claims 1 to 18. 20. A pharmaceutical composition comprising a peptide as claimed in any one of claims 1 to 18 or a peptidomemetic as claimed in claim 19 and a pharmaceutically acceptable excipient. 21. Use of a peptide as claimed in any one of claims 1 to 18 or a peptidomimetic as claimed in claim 19 or a pharmaceutical composition as claimed in claim 20 to inhibit Toll-like receptor 4 (TLR4) signalling. 22. Use as claimed in claim 21 wherein the TLR4 signalling is activated by a pathogen or pathogen component leading to a cytokine response. 23. Use as claimed in claim 22 wherein the TLR4 signalling protein activated is one or more of NFkB. ΙκΒα, IRF3 and p38. 24. Use as claimed in claim 22 or 23 wherein the pathogen is a bacterium or a bacterial component. 25. Use as claimed in claim 24 wherein the bacterial component is lipopolysaccharidc. 26. A method of treatment or prophylaxis of a TLR4-associated disease comprising the step of administering an effective amount of a peptide as claimed in any one of claims 1 to 18 or a peptidomimetic as claimed in claim 19 or a pharmaceutical composition as claimed in claim 20 to a subject. 27. A method as claimed in claim 26 wherein the disease is a disease of the immune system and/or is an inflammatory disease. -62IE 0 9 0 8 7 5 28. A method as claimed in claim 26 or 27 wherein the disease is one or more of: sepsis, rheumatoid arthritis, colitis, multiple sclerosis, irritable bowel disease, cancer, sterile in llam mat ion, pathogen-associated inflammation, kidney ischemia/reperfusion injury, liver ischemia/reperfusion injury, plaque development in atherosclerosis-prone subjects, and acute lung injury. 29. A method of inhibiting TLR4-induced cytokine responses comprising the step of administering an effective amount of a peptide comprising the amino acid sequence of SEQ ID No. 4, SEQ ID No. 20, SEQ ID No. 38, SEQ ID No. 40. SEQ ID No. 41, SEQ ID No. 42. SEQ ID No. 55, SEQ ID No. 56, SEQ ID No. 57, SEQ ID No. 62. SEQ ID No. 68. SEQ ID No. 69, SEQ ID No. 70, SEQ ID No. 71, SEQ ID No. 72, SEQ ID No. 73. SEQ ID No, 74, SEQ ID No. 75, SEQ ID No. 76, SEQ ID No. 77, SEQ ID No. 78, SEQ ID No.79, SEQ ID No. 80, SEQ ID No. 81, SEQ ID No. 82, SEQ ID No. 83, SEQ ID No. 84. SEQ ID No.85, SEQ ID No. 86, SEQ ID No. 87, SEQ ID No. 88, SEQ ID No. 89. SEQ ID No. 90, SEQ ID No. 91, SEQ ID No. 92, SEQ ID No. 93, SEQ ID No. 94. SEQ ID No. 95, SEQ ID No. 96, SEQ ID No. 97, SEQ ID No. 98, SEQ ID No. 99, SEQ ID No. 100, SEQ ID No. 101, SEQ ID No. 102, SEQ ID No. 103, SEQ ID No. 104. SEQ ID No. 105, SEQ ID No. 106, SEQ ID No. 107, SEQ ID No. 108, SEQ ID No. 109. SEQ ID No. 110. SEQ ID No. Ill, SEQ ID No. 112, SEQ ID No. 113, SEQ ID No 114. SEQ ID No. 115. SEQ ID No. 116, or SEQ ID No. 117 to a subject. 30. A method of inhibiting TLR4 induced responses comprising the step of administering an effective amount of a peptidomimetic based on the amino acid sequence of SEQ ID No.
10. 57. A composition comprising a compound and/or a substance identified by the method of any one of claims 50 to 54 and a pharmaceutically acceptable excipient.
IE20090875A 2008-11-17 2009-11-17 A peptide and use thereof IE20090875A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IE20090875A IE20090875A1 (en) 2008-11-17 2009-11-17 A peptide and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IE20080917 2008-11-17
IE20090875A IE20090875A1 (en) 2008-11-17 2009-11-17 A peptide and use thereof

Publications (1)

Publication Number Publication Date
IE20090875A1 true IE20090875A1 (en) 2010-07-07

Family

ID=42340632

Family Applications (1)

Application Number Title Priority Date Filing Date
IE20090875A IE20090875A1 (en) 2008-11-17 2009-11-17 A peptide and use thereof

Country Status (1)

Country Link
IE (1) IE20090875A1 (en)

Similar Documents

Publication Publication Date Title
Piao et al. A decoy peptide that disrupts TIRAP recruitment to TLRs is protective in a murine model of influenza
US9745357B2 (en) Peptides used for treating cancers and, in particular, chronic lymphoid leukaemia
JP5715305B2 (en) Autophagy-inducing peptide
Stack et al. Poxviral protein A46 antagonizes Toll-like receptor 4 signaling by targeting BB loop motifs in Toll-IL-1 receptor adaptor proteins to disrupt receptor: adaptor interactions
JP2013538564A (en) Antiviral agent
US9540427B2 (en) Peptide-based stat inhibitor
KR100565819B1 (en) Compounds that inhibit interaction between signal-transducing proteins and the glgfpdz/dhr domain and uses thereof
Skovbakke et al. The role of formyl peptide receptors for immunomodulatory activities of antimicrobial peptides and peptidomimetics
US8722052B2 (en) Vaccinia virus protein A46 peptide and use thereof
US11351227B2 (en) Chemokine decoy receptors of rodent gammaherpesviruses and uses thereof
CA2526684C (en) Therapeutic peptides and method
US20100016224A1 (en) Compositions and methods for modulating an immune response
US7923545B2 (en) Caterpiller gene family
KR101998191B1 (en) Pharmaceutical composition for preventing or treating sepsis and colorectal cancer comprising Toxoplasma gondii-GRA8 recombinant protein as an active ingredient
CN109593123A (en) A kind of polypeptide and its application derived from RPS23RG1
IE20090875A1 (en) A peptide and use thereof
BOWIE et al. Patent 2744120 Summary
WO2014095977A1 (en) Novel pellino peptide
CN112351993A (en) Modified immunomodulatory peptides
WO2018112226A1 (en) Sharpin-based polypeptides and their uses
KR102666314B1 (en) Recombinant MPT protein derived from MPT63 and MPT64 and use thereof
KR101968253B1 (en) Pharmaceutical composition for preventing or treating listeriosis and candidiasis comprising Toxoplasma gondii-GRA7 recombinant protein as an active ingredient
US20230203107A1 (en) Peptide for treating sepsis derived from rv3364c protein of mycobacterium tuberculosis
US20220378893A1 (en) Recombinant mpt protein derived from mpt63 and mpt64 and use thereof
JP2021534826A (en) Peptide therapeutics and their use for the treatment of cancer

Legal Events

Date Code Title Description
MM9A Patent lapsed through non-payment of renewal fee