HUT46070A - Process for producing polypeptide and dna encoding the same - Google Patents
Process for producing polypeptide and dna encoding the sameInfo
- Publication number
- HUT46070A HUT46070A HU88891A HU89188A HUT46070A HU T46070 A HUT46070 A HU T46070A HU 88891 A HU88891 A HU 88891A HU 89188 A HU89188 A HU 89188A HU T46070 A HUT46070 A HU T46070A
- Authority
- HU
- Hungary
- Prior art keywords
- mutein
- bfgf
- heteroduplex
- approp
- replication
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
A mutein of basic fibroblast growth factor (bFGF) is claimed Also claimed is a recombinant DNA having a base sequence which codes for a mutein of bFGF and a transformant harbouring a vector contg. a recombinant DNA having a base sequence which codes for a mutein of bFGF. - The prodn. of the structural gene which encodes the mutein is carried out by e.g. (a) hybridising with a mutagenic digonucleotide primer a single-stranded DNA comprising 1 strand of the structural gene of bFGF, (b) elongating the primer using DNA polymerase to form a mutational heteroduplex and (c) replicating this mutation heteroduplex. The resulting mutational heteroduplex is used to transform an infectable host organism or cell. In the replication of the heteroduplex by the host, progenies are produced from both chains. Following the replication, the mutant gene is isolated from a progeny of the mutant's chain and is inserted in an approp. vector which is used to transform an approp. host organism or cell.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4221887 | 1987-02-24 | ||
JP4344487 | 1987-02-25 | ||
JP8197787 | 1987-04-02 | ||
JP14751187 | 1987-06-12 | ||
JP20151087 | 1987-08-11 | ||
JP29028387 | 1987-11-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
HUT46070A true HUT46070A (en) | 1988-09-28 |
HU204089B HU204089B (en) | 1991-11-28 |
Family
ID=27550072
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU88891A HU204089B (en) | 1987-02-24 | 1988-02-24 | Process for producing muteins of bfgf |
Country Status (1)
Country | Link |
---|---|
HU (1) | HU204089B (en) |
-
1988
- 1988-02-24 HU HU88891A patent/HU204089B/en unknown
Also Published As
Publication number | Publication date |
---|---|
HU204089B (en) | 1991-11-28 |
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