HUT46070A - Process for producing polypeptide and dna encoding the same - Google Patents

Process for producing polypeptide and dna encoding the same

Info

Publication number
HUT46070A
HUT46070A HU88891A HU89188A HUT46070A HU T46070 A HUT46070 A HU T46070A HU 88891 A HU88891 A HU 88891A HU 89188 A HU89188 A HU 89188A HU T46070 A HUT46070 A HU T46070A
Authority
HU
Hungary
Prior art keywords
mutein
bfgf
heteroduplex
approp
replication
Prior art date
Application number
HU88891A
Other languages
Hungarian (hu)
Other versions
HU204089B (en
Inventor
Masaharu Senoo
Tsutomu Kurokawa
Koichi Igarashi
Reiko Sasada
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Publication of HUT46070A publication Critical patent/HUT46070A/en
Publication of HU204089B publication Critical patent/HU204089B/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A mutein of basic fibroblast growth factor (bFGF) is claimed Also claimed is a recombinant DNA having a base sequence which codes for a mutein of bFGF and a transformant harbouring a vector contg. a recombinant DNA having a base sequence which codes for a mutein of bFGF. - The prodn. of the structural gene which encodes the mutein is carried out by e.g. (a) hybridising with a mutagenic digonucleotide primer a single-stranded DNA comprising 1 strand of the structural gene of bFGF, (b) elongating the primer using DNA polymerase to form a mutational heteroduplex and (c) replicating this mutation heteroduplex. The resulting mutational heteroduplex is used to transform an infectable host organism or cell. In the replication of the heteroduplex by the host, progenies are produced from both chains. Following the replication, the mutant gene is isolated from a progeny of the mutant's chain and is inserted in an approp. vector which is used to transform an approp. host organism or cell.
HU88891A 1987-02-24 1988-02-24 Process for producing muteins of bfgf HU204089B (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP4221887 1987-02-24
JP4344487 1987-02-25
JP8197787 1987-04-02
JP14751187 1987-06-12
JP20151087 1987-08-11
JP29028387 1987-11-17

Publications (2)

Publication Number Publication Date
HUT46070A true HUT46070A (en) 1988-09-28
HU204089B HU204089B (en) 1991-11-28

Family

ID=27550072

Family Applications (1)

Application Number Title Priority Date Filing Date
HU88891A HU204089B (en) 1987-02-24 1988-02-24 Process for producing muteins of bfgf

Country Status (1)

Country Link
HU (1) HU204089B (en)

Also Published As

Publication number Publication date
HU204089B (en) 1991-11-28

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