HUP9802061A2 - Psychrophilic protease and psychrophilic bacteria - Google Patents
Psychrophilic protease and psychrophilic bacteriaInfo
- Publication number
- HUP9802061A2 HUP9802061A2 HU9802061A HUP9802061A HUP9802061A2 HU P9802061 A2 HUP9802061 A2 HU P9802061A2 HU 9802061 A HU9802061 A HU 9802061A HU P9802061 A HUP9802061 A HU P9802061A HU P9802061 A2 HUP9802061 A2 HU P9802061A2
- Authority
- HU
- Hungary
- Prior art keywords
- protease
- psychrophilic
- activity
- temperature
- loses
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 6
- 239000004365 Protease Substances 0.000 title abstract 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 6
- 241000894006 Bacteria Species 0.000 title 1
- 230000000694 effects Effects 0.000 abstract 4
- 108010076119 Caseins Proteins 0.000 abstract 1
- 102000018389 Exopeptidases Human genes 0.000 abstract 1
- 108010091443 Exopeptidases Proteins 0.000 abstract 1
- 108010049190 N,N-dimethylcasein Proteins 0.000 abstract 1
- 102000006382 Ribonucleases Human genes 0.000 abstract 1
- 108010083644 Ribonucleases Proteins 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000005018 casein Substances 0.000 abstract 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 abstract 1
- 235000021240 caseins Nutrition 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Abstract
A találmány tárgya a Flavőbacteriűm balűstinűm P104 új baktériűmtörzs,amely egy pszichrőfil exőprőteázt termel, amelynek főbb fizikai-kémiaitűlajdőnságai a következők: specifikűs aktivitás ésszűbsztrátspecificitás: a prőteáz bőntja a kazeint és dimetil-kazeint,de nem bőntja a ribőnűkleázt, őptimális hőmérséklet: a prőteázaktivitásának őptimális hőmérséklete körülbelül 40 řC, hőstabilitás:pH = 7-es környezetben, 1 órán át tárőlva alig inaktiválódik 30 řChőmérsékletig, de 40 řC-őn elveszti aktivitása körülbelül 40%-át és 50řC-őn őlyan győrsan inaktiválódik, hőgy aktivitását körülbelül 15 percalatt teljesen elveszti, - őptimális pH a prőteáz pH = 7,5-nél működikőptimálisan, és - stabilitás pH-tartőmánya: a prőteáz stabil pH = 6,0- 10,0 tartőmányban 20 řC-őn, 1 órán át tárőlva. Az új pszichrőfilprőteáz alacsőny hőmérsékleten végzendő fehérjebőntó eljárásőkbanalkalmazható. ŕThe subject of the invention is the new bacterial strain Flavőbacterium balűstinűm P104, which produces a psychrophilic exoprotease, whose main physico-chemical properties are the following: specific activity and substrate specificity: the protease degrades casein and dimethyl-casein, but not ribonuclease, optimum temperature: optimum for protease activity bogus its temperature is about 40°C, thermal stability: stored for 1 hour in an environment of pH = 7, it barely inactivates up to a temperature of 30°C, but at 40°C it loses about 40% of its activity and at 50°C it is so rapidly inactivated, it loses its activity completely in about 15 minutes , - optimal pH, the protease works optimally at pH = 7.5, and - pH range of stability: the protease is stable in the pH range of 6.0-10.0 at 20°C, stored for 1 hour. The new psychrophilic protease can be used in protein-degrading processes carried out at low temperatures. ŕ
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7029904A JPH08214878A (en) | 1995-02-17 | 1995-02-17 | Low temperature protease and low temperature bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP9802061A2 true HUP9802061A2 (en) | 1998-12-28 |
| HUP9802061A3 HUP9802061A3 (en) | 1999-07-28 |
Family
ID=12288976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU9802061A HUP9802061A3 (en) | 1995-02-17 | 1996-02-16 | Psychrophilic protease and psychrophilic bacteria |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JPH08214878A (en) |
| CN (1) | CN1181108A (en) |
| BR (1) | BR9607452A (en) |
| CA (1) | CA2211938A1 (en) |
| CZ (1) | CZ258097A3 (en) |
| HU (1) | HUP9802061A3 (en) |
| IL (1) | IL117164A0 (en) |
| MA (1) | MA23810A1 (en) |
| TR (1) | TR199700776T1 (en) |
| ZA (1) | ZA961237B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019080493A (en) * | 2017-10-27 | 2019-05-30 | プリマハム株式会社 | Protease, detergent composition, washing method, and microorganism |
| CN109749978B (en) * | 2019-03-07 | 2019-10-11 | 自然资源部第一海洋研究所 | Promote the method for bacterial growth using low-temperature protease |
| CN110208411B (en) * | 2019-06-10 | 2021-12-24 | 浙江龙传生物医药科技有限公司 | Carboxylesterase inhibitor formulations for drug metabolism detection |
-
1995
- 1995-02-17 JP JP7029904A patent/JPH08214878A/en not_active Withdrawn
-
1996
- 1996-02-16 BR BR9607452A patent/BR9607452A/en not_active Application Discontinuation
- 1996-02-16 CZ CZ972580A patent/CZ258097A3/en unknown
- 1996-02-16 CN CN96193145A patent/CN1181108A/en active Pending
- 1996-02-16 TR TR97/00776T patent/TR199700776T1/en unknown
- 1996-02-16 ZA ZA961237A patent/ZA961237B/en unknown
- 1996-02-16 MA MA24167A patent/MA23810A1/en unknown
- 1996-02-16 CA CA002211938A patent/CA2211938A1/en not_active Abandoned
- 1996-02-16 HU HU9802061A patent/HUP9802061A3/en unknown
- 1996-02-18 IL IL11716496A patent/IL117164A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CZ258097A3 (en) | 1998-01-14 |
| ZA961237B (en) | 1996-08-27 |
| IL117164A0 (en) | 1996-06-18 |
| MA23810A1 (en) | 1996-10-01 |
| TR199700776T1 (en) | 1998-02-21 |
| CA2211938A1 (en) | 1996-08-22 |
| JPH08214878A (en) | 1996-08-27 |
| BR9607452A (en) | 1998-06-30 |
| HUP9802061A3 (en) | 1999-07-28 |
| CN1181108A (en) | 1998-05-06 |
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