HUP2100356A1 - Jelölt exoglikozidáz enzimek és a glikán szekvenálás megközelítése azok immobilizációjával - Google Patents

Jelölt exoglikozidáz enzimek és a glikán szekvenálás megközelítése azok immobilizációjával

Info

Publication number
HUP2100356A1
HUP2100356A1 HU2100356A HUP2100356A HUP2100356A1 HU P2100356 A1 HUP2100356 A1 HU P2100356A1 HU 2100356 A HU2100356 A HU 2100356A HU P2100356 A HUP2100356 A HU P2100356A HU P2100356 A1 HUP2100356 A1 HU P2100356A1
Authority
HU
Hungary
Prior art keywords
tagged
sequencing approach
glycan sequencing
exoglycosidase enzymes
immobilized glycan
Prior art date
Application number
HU2100356A
Other languages
English (en)
Hungarian (hu)
Inventor
Gabor Jarvas
Marton Geza Szigeti
Hajnalka Jankovics
Noemi Kovacs
Robert Farsang
Original Assignee
Pannon Egyetem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pannon Egyetem filed Critical Pannon Egyetem
Priority to HU2100356A priority Critical patent/HUP2100356A1/hu
Priority to JP2024522434A priority patent/JP2024538794A/ja
Priority to PCT/HU2022/050074 priority patent/WO2023062397A1/en
Priority to CA3235359A priority patent/CA3235359A1/en
Priority to US18/701,364 priority patent/US20240417708A1/en
Priority to EP22880475.3A priority patent/EP4416281A4/en
Publication of HUP2100356A1 publication Critical patent/HUP2100356A1/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
HU2100356A 2021-10-14 2021-10-14 Jelölt exoglikozidáz enzimek és a glikán szekvenálás megközelítése azok immobilizációjával HUP2100356A1 (hu)

Priority Applications (6)

Application Number Priority Date Filing Date Title
HU2100356A HUP2100356A1 (hu) 2021-10-14 2021-10-14 Jelölt exoglikozidáz enzimek és a glikán szekvenálás megközelítése azok immobilizációjával
JP2024522434A JP2024538794A (ja) 2021-10-14 2022-10-14 タグ化エキソグリコシダーゼ酵素および固定化グリカン配列決定法
PCT/HU2022/050074 WO2023062397A1 (en) 2021-10-14 2022-10-14 Tagged exoglycosidase enzymes and immobilized glycan sequencing approach
CA3235359A CA3235359A1 (en) 2021-10-14 2022-10-14 Tagged exoglycosidase enzymes and immobilized glycan sequencing approach
US18/701,364 US20240417708A1 (en) 2021-10-14 2022-10-14 Tagged exoglycosidase enzymes and immobilized glycan sequencing approach
EP22880475.3A EP4416281A4 (en) 2021-10-14 2022-10-14 Labeled exoglycoside enzymes and sequencing approach for immobilized glycans

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU2100356A HUP2100356A1 (hu) 2021-10-14 2021-10-14 Jelölt exoglikozidáz enzimek és a glikán szekvenálás megközelítése azok immobilizációjával

Publications (1)

Publication Number Publication Date
HUP2100356A1 true HUP2100356A1 (hu) 2023-09-28

Family

ID=89993442

Family Applications (1)

Application Number Title Priority Date Filing Date
HU2100356A HUP2100356A1 (hu) 2021-10-14 2021-10-14 Jelölt exoglikozidáz enzimek és a glikán szekvenálás megközelítése azok immobilizációjával

Country Status (6)

Country Link
US (1) US20240417708A1 (enExample)
EP (1) EP4416281A4 (enExample)
JP (1) JP2024538794A (enExample)
CA (1) CA3235359A1 (enExample)
HU (1) HUP2100356A1 (enExample)
WO (1) WO2023062397A1 (enExample)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20075030A0 (fi) * 2007-01-18 2007-01-18 Suomen Punainen Risti Veripalv Menetelmä solujen modifioimiseksi
ES2626080T3 (es) * 2007-04-16 2017-07-21 Momenta Pharmaceuticals, Inc. Caracterización de N-glicanos utilizando exoglicosidasas

Also Published As

Publication number Publication date
EP4416281A1 (en) 2024-08-21
JP2024538794A (ja) 2024-10-23
WO2023062397A1 (en) 2023-04-20
EP4416281A4 (en) 2025-10-29
CA3235359A1 (en) 2023-04-20
US20240417708A1 (en) 2024-12-19

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